Spg038755 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg038755
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionUnknown protein
Locationscaffold12: 5166437 .. 5166943 (-)
RNA-Seq ExpressionSpg038755
SyntenySpg038755
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTGTGCAGGCCACCAGAAAATGAACAAAGAATGGTCAATTAAAGCCCTCGAAGCTCTAGCCATTGTCGAAGGATTAAAAGCATACCTGTTGCTGAAGGAGAAAGATGATTTCCGACATCTAATTCCCCCAATTTGGGTCGAATCCGACGCCGCCGAAGTGATTAAATTCCTCAATCTAGAGAGCGAAGACCTTTCGGAAACCAAACACATTCTGGAAGAAGCGGCTGATCTCGGTCGCAAGGCAGGGACATTGGCGTTCTCGTTCTGTCCAAGAGAATGCAATCGCGTGGCTCACACCCTTGCTCGAGCTGCAAGTTCGTCCCCAACATCTAGCATAGTTTACTCTAGCTTTCGTTTCTCTTTGATCGCCAATTTTGAGCAATCCCCATATTCTTCTCTCGAAGATGTCGTTTTTTGGAGGGATTCTATTGTTCCCACTTGGCTTTCCTTTGCTATTAATGAGGATTTTTGTATACCTAACTTGGGTTCTCTTTATTATTGA

mRNA sequence

ATGATTTGTGCAGGCCACCAGAAAATGAACAAAGAATGGTCAATTAAAGCCCTCGAAGCTCTAGCCATTGTCGAAGGATTAAAAGCATACCTGTTGCTGAAGGAGAAAGATGATTTCCGACATCTAATTCCCCCAATTTGGGTCGAATCCGACGCCGCCGAAGTGATTAAATTCCTCAATCTAGAGAGCGAAGACCTTTCGGAAACCAAACACATTCTGGAAGAAGCGGCTGATCTCGGTCGCAAGGCAGGGACATTGGCGTTCTCGTTCTGTCCAAGAGAATGCAATCGCGTGGCTCACACCCTTGCTCGAGCTGCAAGTTCGTCCCCAACATCTAGCATAGTTTACTCTAGCTTTCGTTTCTCTTTGATCGCCAATTTTGAGCAATCCCCATATTCTTCTCTCGAAGATGTCGTTTTTTGGAGGGATTCTATTGTTCCCACTTGGCTTTCCTTTGCTATTAATGAGGATTTTTGTATACCTAACTTGGGTTCTCTTTATTATTGA

Coding sequence (CDS)

ATGATTTGTGCAGGCCACCAGAAAATGAACAAAGAATGGTCAATTAAAGCCCTCGAAGCTCTAGCCATTGTCGAAGGATTAAAAGCATACCTGTTGCTGAAGGAGAAAGATGATTTCCGACATCTAATTCCCCCAATTTGGGTCGAATCCGACGCCGCCGAAGTGATTAAATTCCTCAATCTAGAGAGCGAAGACCTTTCGGAAACCAAACACATTCTGGAAGAAGCGGCTGATCTCGGTCGCAAGGCAGGGACATTGGCGTTCTCGTTCTGTCCAAGAGAATGCAATCGCGTGGCTCACACCCTTGCTCGAGCTGCAAGTTCGTCCCCAACATCTAGCATAGTTTACTCTAGCTTTCGTTTCTCTTTGATCGCCAATTTTGAGCAATCCCCATATTCTTCTCTCGAAGATGTCGTTTTTTGGAGGGATTCTATTGTTCCCACTTGGCTTTCCTTTGCTATTAATGAGGATTTTTGTATACCTAACTTGGGTTCTCTTTATTATTGA

Protein sequence

MICAGHQKMNKEWSIKALEALAIVEGLKAYLLLKEKDDFRHLIPPIWVESDAAEVIKFLNLESEDLSETKHILEEAADLGRKAGTLAFSFCPRECNRVAHTLARAASSSPTSSIVYSSFRFSLIANFEQSPYSSLEDVVFWRDSIVPTWLSFAINEDFCIPNLGSLYY
Homology
BLAST of Spg038755 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 59.3 bits (142), Expect = 3.7e-05
Identity = 42/119 (35.29%), Postives = 66/119 (55.46%), Query Frame = 0

Query: 1   MICAGHQKMNKEWSIKALEALAIVEGLKAYLLLKEKDDFRHLIPPIWVESDAAEVIKFLN 60
           ++ AG++ +    ++K LEA AI+EGL+    L        ++ P+ +E+D+AEV   LN
Sbjct: 107 IVLAGNRFVEACNNVKLLEASAILEGLRNLTNL-------GVLRPLHIETDSAEVESLLN 166

Query: 61  LESEDLSETKHILEEAADLGRKAGTLAFSFCPRECNRVAHTLARAASSSPTSSIVYSSF 120
            + EDL++T  ++EE  +L      LAF+   RE N  AH+LA+ AS    S I    F
Sbjct: 167 RKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQRASVLRESMIWVDCF 218

BLAST of Spg038755 vs. NCBI nr
Match: KAE8776165.1 (hypothetical protein D1007_51201 [Hordeum vulgare])

HSP 1 Score: 55.5 bits (132), Expect = 5.3e-04
Identity = 37/97 (38.14%), Postives = 50/97 (51.55%), Query Frame = 0

Query: 15  IKALEALAIVEGLKAYLLLKEKDDFRHLIPPIWVESDAAEVIKFLNLESEDLSETKHILE 74
           + + EA A+ +GL   LL  E       +  I VESD  +VI  + L+   L     + E
Sbjct: 163 VSSAEARALRDGL---LLASEVG-----VQKIIVESDCQDVIDTMLLDDNSLGPAAAVYE 222

Query: 75  EAADLGRKAGTLAFSFCPRECNRVAHTLARAASSSPT 112
           E + L +    + FSFCPRE N VAHTLAR A  + T
Sbjct: 223 ECSFLAKNFSLIQFSFCPREANMVAHTLARFAEPTRT 251

BLAST of Spg038755 vs. NCBI nr
Match: KAF7821986.1 (putative ribonuclease H-like domain, reverse transcriptase zinc-binding domain-containing protein [Senna tora])

HSP 1 Score: 55.1 bits (131), Expect = 6.9e-04
Identity = 36/112 (32.14%), Postives = 61/112 (54.46%), Query Frame = 0

Query: 2   ICAGHQKMNKEWSIKALEALAIVEGLKAYLLLKEKDDFRHLIPPIWVESDAAEVIKFLNL 61
           + A  +K+    +I+ +EALA++EG+K   L+            I +ESDA  V+  LN 
Sbjct: 525 LMAMSKKVKTGATIEYVEALAVLEGMKQAQLM--------CCNRIIIESDAQRVVNLLNK 584

Query: 62  ESEDLSETKHILEEAADLGRKAGTLAFSFCPRECNRVAHTLARAASSSPTSS 114
            + D+S    ++EE  +L     +++F++  RE N VAHTLA +  + P +S
Sbjct: 585 GNIDISYLGLVIEEILELKSHFSSISFNWVKREANMVAHTLAHSVVNFPYNS 628

BLAST of Spg038755 vs. NCBI nr
Match: KAG6599977.1 (hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 54.7 bits (130), Expect = 9.0e-04
Identity = 31/91 (34.07%), Postives = 52/91 (57.14%), Query Frame = 0

Query: 2   ICAGHQKMNKEWSIKALEALAIVEGLKAYLLLKEKDDFRHLIPPIWVESDAAEVIKFLNL 61
           IC   +++ ++W +K LE  A++EGLK YLL++E   + H  P I++     E+ + LN 
Sbjct: 35  ICIECKELRRKWLVKMLEGKAMIEGLKTYLLIRETVSYSHR-PIIFL-----ELARILNN 94

Query: 62  ESEDLSETKHILEEAADLGRKAGTLAFSFCP 93
              DL E  ++++E  DL   AG ++F   P
Sbjct: 95  THMDLIELSNVVDEIFDLELLAGIISFFQVP 119

BLAST of Spg038755 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.8e-05
Identity = 42/119 (35.29%), Postives = 66/119 (55.46%), Query Frame = 0

Query: 1   MICAGHQKMNKEWSIKALEALAIVEGLKAYLLLKEKDDFRHLIPPIWVESDAAEVIKFLN 60
           ++ AG++ +    ++K LEA AI+EGL+    L        ++ P+ +E+D+AEV   LN
Sbjct: 107 IVLAGNRFVEACNNVKLLEASAILEGLRNLTNL-------GVLRPLHIETDSAEVESLLN 166

Query: 61  LESEDLSETKHILEEAADLGRKAGTLAFSFCPRECNRVAHTLARAASSSPTSSIVYSSF 120
            + EDL++T  ++EE  +L      LAF+   RE N  AH+LA+ AS    S I    F
Sbjct: 167 RKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQRASVLRESMIWVDCF 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155262.13.7e-0535.29uncharacterized protein LOC111022403 [Momordica charantia][more]
KAE8776165.15.3e-0438.14hypothetical protein D1007_51201 [Hordeum vulgare][more]
KAF7821986.16.9e-0432.14putative ribonuclease H-like domain, reverse transcriptase zinc-binding domain-c... [more]
KAG6599977.19.0e-0434.07hypothetical protein SDJN03_05210, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DNV91.8e-0535.29uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 56..76
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 2..106
e-value: 1.1E-14
score: 54.3
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 2..113
e-value: 9.7E-7
score: 30.8
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 14..104
e-value: 8.13877E-11
score: 54.6276
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 13..110

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg038755.1Spg038755.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity