Spg038696 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg038696
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Calcium-binding EF-hand family protein
Location	scaffold12: 2770142 .. 2773539 (-)
RNA-Seq Expression	Spg038696
Synteny	Spg038696

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGGGAAGGAGGATTCAGTCGACCCCTTGTTGTTGAATCCGATGCTTCTAAGGTGATTAAAGTGCTCATTGAGAGCGCAGAAGATCGATCGGAGCTTTCTTTGTTTACAAGCGCGATCGAAGATTTGGTACAAGGAGAAAATGTCCCCTTTGCGAAATGCTCAAGAGAGTATTTTTTTGGCCCATAGTCTTGCTAGGGTAGCCATCTCTAATGACAACTTTGTATTTTTTGGACGCTCTTCTTTCCCGTTGTCGGAAGAGGAAGCGTTTGTTGGCGAGGTTAGAATCCCTTCGTAGGTTTTGGCGATTTTAGCCGCGAACGATGTTTGTTATGTTTTTCCTTATTAATGCATTTCTTTTCTAAAAAAAAAAAGTCAATTAGATATGTCAATATGAGCTTTAACTGGTTAAAGTATTGTATACTCGTCAAAAGGTAAGAGTTTCGCTTAAATCTTTGTTCCCACAAAGGAAAAAAAAGAGTGAATTAGAGTTGGGATTGAAAATCGAGTTGGTAGAGTAAAATTAGTAAGAAAGTGTGATTGTTATTTTATGGGATTTTTCCAAGTAGTTGATATGACATGTTGATCCATGTAAATTTTATTTAATGAAGTGTTATGTGTCTCTCGACGTAAAATAAATATAAATAAATTCAAAATTTAAAGGCATAATATAGACTTTTTTTTATATGTAAGGGGATCGCTTAATATAGTGTCAAAGTTTATAGACAAAATTAGCTAAATATAGTGTTCAAAGTTTATAGACAAAATTATGGATACAAGAATGAAAATGAAAAACCCAACTAATTAGGGTGTGTTTGGTTTAACTTTTCAAATGTTTAATTTTGAAAATAAATCATTTTGAAAAAATTTACAGTGTTTGGCAACCACTAAAAATGACTTATTAGAAAATGAGATTGATAAATAAATCATTGAGAGAAACACTTGAAACCAACTTTTTAAAAAAAGACTTCTAAGTAAATAATAGCTTGTGTAGAAAAACATTTGTGAGGAAATACAACCAAACATATGAGTGTTTAAATTCTAAACAATTTTTATACAAAATGTTTTTTAAAAAATGTATTCTGAAAAACATTTAAATTAAAATGTATTCCAAACGGACCCTTAATAAAGACCAAATTTCATAACCATTTTATTTTTTTGTTTTTTTATTTTTAAATTTAAGCTCATAAACACTACTTGGATCAATGTATTTCTTTTGTTTGTTGTTTACTTTTTAAAATTATGTTCAAAATTTAAGCGAAAATTTGAAAACTAAAAAAAAAAAAAAAAAAAAAAAGTAGTTTTTAAAAACTTGTGAGTAAAATACACTTTTGGTTACTAGAATTTAAATTTAATTTCTATTTGGTCTCATTTTTAAAACAAATACTTTTGGTCATTGATATTAATTTATGATGTTTCAAAAGTGAGGAATGCCGAGGAGTAGAAGGAGAGGTGCTAGCCAAAGCGAGCATAACTCTATATCCTTGACCAAGAGGTCAGAGATTCAAATCCCCTCACCTCCATATGATGTTGTACTAAAAAAAAAGAGAGGTGCTCTTCTTCTCTACTCTTAGACTTTGTTGATATGTATTTATTAAATGAGTTGAATTATTTTTTTTATATATTTTATTTTTATACTTAGGGATAAATGAGATTCTGGTTAGATTCCAATTTCAACCTCGAAGAACTAACAAGAAATCAAATTTAAAAGTCAGAGATCAAAGATGTCACTTTTAAAATATTAAGGATAAAAAAGAAACAAAATTCAAATTTCAAGGACCAAAAGTGTTAGTTTTGAAAACTCAAAAGACCAAATAGAAACTAAGTTCAAATTTCAGGAACCAAAATTGTATTTTATCTAAAATCTGTTTTTATTTTTGAAATTTGACTAATGATTCAAATATGTTTTTAAGAAAGATGAAAAACATACCTAAGAAATAGCGATAAAACACATTATTCAAAAATAGAAACTAAAAAACAAGATATTATTAAACAGACAAGTTTTTAATAAACTAGGTTCAAGTCCGTCTCAGTTGAGCGATTTAAAAAAAAAATAGGCATAAAATTTTTAATTTCCTTAAACTAACACATTGGTTTTATAACATCCATCAACCTAAAATCAACCCAATATACCTCAAGACAAAATTTATAATTTAGCTTAAAAATCACTTTGGGTCCCTAAACTTTCATAAAAGTAACAATTTGGTCCTTGAACTTTAATTTGTAACGACTTAGTCCTTGTACATTTAATTTTGTAACAATTTAGTCCCTGAACTTTTAATAAGTAACAATTTAGCCCATATACTTTCAAATTTATAACAATTTTGTCCCTATACTTTTAAATTTGTAACAATTTAATCCTTGTCGCCAAAAAATTCATCAAAATTAAATGTCGATTTTTATTATTTAACTATTAAACTTTGTAGCTTATTAACATATTGAGTCCCAAATTAGTTTATTAATCTATCTAGGTAAGAAATTTCATTAAATCTTTACATTAATTTTTCACGATAGGAACTAAATCGTTACAAATTTGAAAGTACATGGACTAAATTGTTACTTATTAAAGTTCAGGGACTAAATTGTTATAAAATTGAAAGTACAGAGACTAAATCGTTACAAAATTGAAAGTATAGAGACTAAATCGTTACAAATCAAAGTTCAGGGACTAAATGGTTACTTTCATAATTTAACCAAAAGAGAAAAAAAAAAAAAAAAAAAGCAAATTAAACACATGAGTAAATTCACTCAATGAATCAATCTAAAAGAATTAGGGGTTAAATTTAGTAAAAAAAATCACTCCAAAAAAAAAAAAAACTTCAAACATTTTCCCCAAAAACAAGTAGAAAGTTACATCAAGATCAAAATGCAATGATGTGATCCGAGAAACTCTAGCGATCAAGAACATGTCGCCATTACTCCATTTGGCTCAGCAAATTACAAATCCAATTGCCCTAACTTGAGCAAAAATCAGTTGAGAGCGAAGAACAAGGAGGCGACACCAGGTGGTTCAAATGCCAACGATTTAGGGCAAAATCGCGATTATTAAAGGAGGATGAAAATCGAAGACGAGAAGAAGAAGAAGAAGAAGAATTTGTTCCAAATCGAATCGAATCAAATCAAATCAAACCTCCAGCAACAATAATGGCGGACAATGGCGTGAACACGGCCGAATGCGAGAGGATCTTCAAGCGATTCGACGCGAACGGCGACGGGCAGATCTCGGCGGCGGAGCTCGGCGACGCGTTGAACGGATTCGGAGTGAGCTCCGAGGACGCGAAGAGGATGATGGACGCCATCGACAAAGATGGCGATGGCTTCATTTCGTTCCTAGAATTCGCCGATTTCGCCAAGGATAATCGCGCCTTGATGAAGGATTTTGCCAAGGCGTTTTAG

mRNA sequence

ATGTCGGGGAAGGAGGATTCAGTCGACCCCTTGTTGTTGAATCCGATGCTTCTAAGGGCAAAATCGCGATTATTAAAGGAGGATGAAAATCGAAGACGAGAAGAAGAAGAAGAAGAAGAATTTGTTCCAAATCGAATCGAATCAAATCAAATCAAACCTCCAGCAACAATAATGGCGGACAATGGCGTGAACACGGCCGAATGCGAGAGGATCTTCAAGCGATTCGACGCGAACGGCGACGGGCAGATCTCGGCGGCGGAGCTCGGCGACGCGTTGAACGGATTCGGAGTGAGCTCCGAGGACGCGAAGAGGATGATGGACGCCATCGACAAAGATGGCGATGGCTTCATTTCGTTCCTAGAATTCGCCGATTTCGCCAAGGATAATCGCGCCTTGATGAAGGATTTTGCCAAGGCGTTTTAG

Coding sequence (CDS)

ATGTCGGGGAAGGAGGATTCAGTCGACCCCTTGTTGTTGAATCCGATGCTTCTAAGGGCAAAATCGCGATTATTAAAGGAGGATGAAAATCGAAGACGAGAAGAAGAAGAAGAAGAAGAATTTGTTCCAAATCGAATCGAATCAAATCAAATCAAACCTCCAGCAACAATAATGGCGGACAATGGCGTGAACACGGCCGAATGCGAGAGGATCTTCAAGCGATTCGACGCGAACGGCGACGGGCAGATCTCGGCGGCGGAGCTCGGCGACGCGTTGAACGGATTCGGAGTGAGCTCCGAGGACGCGAAGAGGATGATGGACGCCATCGACAAAGATGGCGATGGCTTCATTTCGTTCCTAGAATTCGCCGATTTCGCCAAGGATAATCGCGCCTTGATGAAGGATTTTGCCAAGGCGTTTTAG

Protein sequence

MSGKEDSVDPLLLNPMLLRAKSRLLKEDENRRREEEEEEEFVPNRIESNQIKPPATIMADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFGVSSEDAKRMMDAIDKDGDGFISFLEFADFAKDNRALMKDFAKAF

Homology

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BLAST of Spg038696 vs. NCBI nr
Match: XP_016902062.1 (PREDICTED: polcalcin Bra n 2-like [Cucumis melo])

HSP 1 Score: 154.1 bits (388), Expect = 9.1e-34
Identity = 75/83 (90.36%), Postives = 81/83 (97.59%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFGVSSEDAKRMMDAIDKDGDGFI 117
           MAD+GVN+A+CERIFKRFDANGDG+ISA ELGDALNGFGVSSEDAKRMMDAIDKDGDG+I
Sbjct: 1   MADSGVNSADCERIFKRFDANGDGKISATELGDALNGFGVSSEDAKRMMDAIDKDGDGYI 60

Query: 118 SFLEFADFAKDNRALMKDFAKAF 141
           SF EF+DFAKDNRALMKDFAKAF
Sbjct: 61  SFQEFSDFAKDNRALMKDFAKAF 83

[Close]

BLAST of Spg038696 vs. NCBI nr
Match: XP_022948513.1 (probable calcium-binding protein CML28 [Cucurbita moschata] >XP_022998721.1 probable calcium-binding protein CML28 [Cucurbita maxima] >XP_023523664.1 probable calcium-binding protein CML28 [Cucurbita pepo subsp. pepo] >KAG6607008.1 Polcalcin Nic t 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036707.1 Polcalcin Nic t 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 152.9 bits (385), Expect = 2.0e-33
Identity = 76/83 (91.57%), Postives = 79/83 (95.18%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFGVSSEDAKRMMDAIDKDGDGFI 117
           MADNGV++AECERIFKRFDANGDGQISA ELGDALNG GVSSEDAKRMMDAIDKDGDGFI
Sbjct: 1   MADNGVDSAECERIFKRFDANGDGQISATELGDALNGIGVSSEDAKRMMDAIDKDGDGFI 60

Query: 118 SFLEFADFAKDNRALMKDFAKAF 141
           SF EF +FAKDNRALMKDFAKAF
Sbjct: 61  SFQEFFEFAKDNRALMKDFAKAF 83

[Close]

BLAST of Spg038696 vs. NCBI nr
Match: XP_038874793.1 (probable calcium-binding protein CML28 [Benincasa hispida])

HSP 1 Score: 146.4 bits (368), Expect = 1.9e-31
Identity = 72/83 (86.75%), Postives = 79/83 (95.18%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFGVSSEDAKRMMDAIDKDGDGFI 117
           MA++GV+ A+CERIFKRFDANGDG+ISA ELGDALNGFGVS+EDAKRMMDAIDKDGDG I
Sbjct: 1   MAESGVSAADCERIFKRFDANGDGKISATELGDALNGFGVSAEDAKRMMDAIDKDGDGCI 60

Query: 118 SFLEFADFAKDNRALMKDFAKAF 141
           SF EFA+FAKDNRALMKDFAKAF
Sbjct: 61  SFEEFAEFAKDNRALMKDFAKAF 83

[Close]

BLAST of Spg038696 vs. NCBI nr
Match: XP_004149790.1 (probable calcium-binding protein CML28 [Cucumis sativus] >KGN57835.1 hypothetical protein Csa_010503 [Cucumis sativus])

HSP 1 Score: 146.0 bits (367), Expect = 2.5e-31
Identity = 73/83 (87.95%), Postives = 78/83 (93.98%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFGVSSEDAKRMMDAIDKDGDGFI 117
           MAD+GVN+A+ ERIFKRFDANGDG+ISA ELGDALN FGVSSEDAKRMMDAIDKDGDG+I
Sbjct: 1   MADSGVNSADLERIFKRFDANGDGKISATELGDALNEFGVSSEDAKRMMDAIDKDGDGYI 60

Query: 118 SFLEFADFAKDNRALMKDFAKAF 141
           SF EF DFAKDNRALMKDFAKAF
Sbjct: 61  SFQEFFDFAKDNRALMKDFAKAF 83

[Close]

BLAST of Spg038696 vs. NCBI nr
Match: XP_023526616.1 (probable calcium-binding protein CML28 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 139.8 bits (351), Expect = 1.8e-29
Identity = 70/83 (84.34%), Postives = 76/83 (91.57%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFGVSSEDAKRMMDAIDKDGDGFI 117
           MA++ V+ AECERIFKRFDANGDG ISAAEL DALNG GV++E+AKRMMDAIDKDGDGFI
Sbjct: 1   MAESDVSAAECERIFKRFDANGDGMISAAELVDALNGLGVNAEEAKRMMDAIDKDGDGFI 60

Query: 118 SFLEFADFAKDNRALMKDFAKAF 141
           S  EFADFAKDNRALMKDFAKAF
Sbjct: 61  SLEEFADFAKDNRALMKDFAKAF 83

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BLAST of Spg038696 vs. ExPASy Swiss-Prot
Match: Q9SRP7 (Probable calcium-binding protein CML28 OS=Arabidopsis thaliana OX=3702 GN=CML28 PE=3 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 9.2e-21
Identity = 56/84 (66.67%), Postives = 62/84 (73.81%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFG-VSSEDAKRMMDAIDKDGDGF 117
           MAD     AE +RIFK+FDANGDG+ISAAELGDAL   G V+ ED KRMM  ID DGDG+
Sbjct: 1   MAD-ATEKAEHDRIFKKFDANGDGKISAAELGDALKNLGSVTHEDIKRMMAEIDTDGDGY 60

Query: 118 ISFLEFADFAKDNRALMKDFAKAF 141
           IS+ EF DFA  NR LMKD AK F
Sbjct: 61  ISYQEFIDFASANRGLMKDVAKIF 83

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BLAST of Spg038696 vs. ExPASy Swiss-Prot
Match: P69199 (Polcalcin Bra r 2 OS=Brassica campestris OX=3711 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 2.7e-20
Identity = 54/84 (64.29%), Postives = 63/84 (75.00%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFG-VSSEDAKRMMDAIDKDGDGF 117
           MAD     AE +RIFK+FDANGDG+ISA+ELGDAL   G V+ +D KRMM  ID DGDG+
Sbjct: 1   MAD-ATEKAEHDRIFKKFDANGDGKISASELGDALKNLGSVTHDDIKRMMAEIDTDGDGY 60

Query: 118 ISFLEFADFAKDNRALMKDFAKAF 141
           IS+ EF+DFA  NR LMKD AK F
Sbjct: 61  ISYQEFSDFASANRGLMKDVAKIF 83

[Close]

BLAST of Spg038696 vs. ExPASy Swiss-Prot
Match: P69198 (Polcalcin Bra n 2 OS=Brassica napus OX=3708 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 2.7e-20
Identity = 54/84 (64.29%), Postives = 63/84 (75.00%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFG-VSSEDAKRMMDAIDKDGDGF 117
           MAD     AE +RIFK+FDANGDG+ISA+ELGDAL   G V+ +D KRMM  ID DGDG+
Sbjct: 1   MAD-ATEKAEHDRIFKKFDANGDGKISASELGDALKNLGSVTHDDIKRMMAEIDTDGDGY 60

Query: 118 ISFLEFADFAKDNRALMKDFAKAF 141
           IS+ EF+DFA  NR LMKD AK F
Sbjct: 61  ISYQEFSDFASANRGLMKDVAKIF 83

[Close]

BLAST of Spg038696 vs. ExPASy Swiss-Prot
Match: Q39419 (Polcalcin Bet v 4 OS=Betula pendula OX=3505 GN=BETV4 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.3e-19
Identity = 51/82 (62.20%), Postives = 61/82 (74.39%), Query Frame = 0


Query: 60  DNGVNTAECERIFKRFDANGDGQISAAELGDALNGFG-VSSEDAKRMMDAIDKDGDGFIS 119
           D+  + AE ERIFKRFDANGDG+ISAAELG+AL   G ++ ++ K MM  ID DGDGFIS
Sbjct: 4   DHPQDKAERERIFKRFDANGDGKISAAELGEALKTLGSITPDEVKHMMAEIDTDGDGFIS 63

Query: 120 FLEFADFAKDNRALMKDFAKAF 141
           F EF DF + NR L+KD AK F
Sbjct: 64  FQEFTDFGRANRGLLKDVAKIF 85

[Close]

BLAST of Spg038696 vs. ExPASy Swiss-Prot
Match: O81092 (Polcalcin Ole e 3 OS=Olea europaea OX=4146 GN=OLE3 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 3.9e-19
Identity = 52/84 (61.90%), Postives = 62/84 (73.81%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFG-VSSEDAKRMMDAIDKDGDGF 117
           MAD+    AE ERIFKRFDANGDG+IS++ELG+ L   G V+ E+ +RMM  ID DGDGF
Sbjct: 1   MADDPQEVAEHERIFKRFDANGDGKISSSELGETLKTLGSVTPEEIQRMMAEIDTDGDGF 60

Query: 118 ISFLEFADFAKDNRALMKDFAKAF 141
           ISF EF  FA+ NR L+KD AK F
Sbjct: 61  ISFEEFTVFARANRGLVKDVAKIF 84

[Close]

BLAST of Spg038696 vs. ExPASy TrEMBL
Match: A0A1S4E1G1 (polcalcin Bra n 2-like OS=Cucumis melo OX=3656 GN=LOC103496835 PE=4 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 4.4e-34
Identity = 75/83 (90.36%), Postives = 81/83 (97.59%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFGVSSEDAKRMMDAIDKDGDGFI 117
           MAD+GVN+A+CERIFKRFDANGDG+ISA ELGDALNGFGVSSEDAKRMMDAIDKDGDG+I
Sbjct: 1   MADSGVNSADCERIFKRFDANGDGKISATELGDALNGFGVSSEDAKRMMDAIDKDGDGYI 60

Query: 118 SFLEFADFAKDNRALMKDFAKAF 141
           SF EF+DFAKDNRALMKDFAKAF
Sbjct: 61  SFQEFSDFAKDNRALMKDFAKAF 83

[Close]

BLAST of Spg038696 vs. ExPASy TrEMBL
Match: A0A6J1KB11 (probable calcium-binding protein CML28 OS=Cucurbita maxima OX=3661 GN=LOC111493300 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 9.8e-34
Identity = 76/83 (91.57%), Postives = 79/83 (95.18%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFGVSSEDAKRMMDAIDKDGDGFI 117
           MADNGV++AECERIFKRFDANGDGQISA ELGDALNG GVSSEDAKRMMDAIDKDGDGFI
Sbjct: 1   MADNGVDSAECERIFKRFDANGDGQISATELGDALNGIGVSSEDAKRMMDAIDKDGDGFI 60

Query: 118 SFLEFADFAKDNRALMKDFAKAF 141
           SF EF +FAKDNRALMKDFAKAF
Sbjct: 61  SFQEFFEFAKDNRALMKDFAKAF 83

[Close]

BLAST of Spg038696 vs. ExPASy TrEMBL
Match: A0A6J1G9I0 (probable calcium-binding protein CML28 OS=Cucurbita moschata OX=3662 GN=LOC111452170 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 9.8e-34
Identity = 76/83 (91.57%), Postives = 79/83 (95.18%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFGVSSEDAKRMMDAIDKDGDGFI 117
           MADNGV++AECERIFKRFDANGDGQISA ELGDALNG GVSSEDAKRMMDAIDKDGDGFI
Sbjct: 1   MADNGVDSAECERIFKRFDANGDGQISATELGDALNGIGVSSEDAKRMMDAIDKDGDGFI 60

Query: 118 SFLEFADFAKDNRALMKDFAKAF 141
           SF EF +FAKDNRALMKDFAKAF
Sbjct: 61  SFQEFFEFAKDNRALMKDFAKAF 83

[Close]

BLAST of Spg038696 vs. ExPASy TrEMBL
Match: A0A0A0L775 (Putative calcium-binding protein CML28 OS=Cucumis sativus OX=3659 GN=Csa_3G345360 PE=4 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.2e-31
Identity = 73/83 (87.95%), Postives = 78/83 (93.98%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFGVSSEDAKRMMDAIDKDGDGFI 117
           MAD+GVN+A+ ERIFKRFDANGDG+ISA ELGDALN FGVSSEDAKRMMDAIDKDGDG+I
Sbjct: 1   MADSGVNSADLERIFKRFDANGDGKISATELGDALNEFGVSSEDAKRMMDAIDKDGDGYI 60

Query: 118 SFLEFADFAKDNRALMKDFAKAF 141
           SF EF DFAKDNRALMKDFAKAF
Sbjct: 61  SFQEFFDFAKDNRALMKDFAKAF 83

[Close]

BLAST of Spg038696 vs. ExPASy TrEMBL
Match: A0A6J1J5E7 (polcalcin Syr v 3-like OS=Cucurbita maxima OX=3661 GN=LOC111481518 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.5e-29
Identity = 74/112 (66.07%), Postives = 91/112 (81.25%), Query Frame = 0


Query: 29  ENRRREEEEEEEFVPNRIESNQIKPPATIMADNGVNTAECERIFKRFDANGDGQISAAEL 88
           E R+R++    E   +R+++  +   A  MA++ ++ AECERIFKRFDANGDG ISAAEL
Sbjct: 2   EERKRKKRRVGE-RRSRLQARLLGIEAVEMAESDISAAECERIFKRFDANGDGMISAAEL 61

Query: 89  GDALNGFGVSSEDAKRMMDAIDKDGDGFISFLEFADFAKDNRALMKDFAKAF 141
           GDALNG GV++E+AKRMMDAIDKDGDGFIS+ EFADF K+NRALMKDFAKAF
Sbjct: 62  GDALNGLGVNAEEAKRMMDAIDKDGDGFISWEEFADFTKENRALMKDFAKAF 112

[Close]

BLAST of Spg038696 vs. TAIR 10
Match: AT3G03430.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 101.3 bits (251), Expect = 6.5e-22
Identity = 56/84 (66.67%), Postives = 62/84 (73.81%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFG-VSSEDAKRMMDAIDKDGDGF 117
           MAD     AE +RIFK+FDANGDG+ISAAELGDAL   G V+ ED KRMM  ID DGDG+
Sbjct: 1   MAD-ATEKAEHDRIFKKFDANGDGKISAAELGDALKNLGSVTHEDIKRMMAEIDTDGDGY 60

Query: 118 ISFLEFADFAKDNRALMKDFAKAF 141
           IS+ EF DFA  NR LMKD AK F
Sbjct: 61  ISYQEFIDFASANRGLMKDVAKIF 83

[Close]

BLAST of Spg038696 vs. TAIR 10
Match: AT5G17480.1 (pollen calcium-binding protein 1 )

HSP 1 Score: 93.2 bits (230), Expect = 1.8e-19
Identity = 53/84 (63.10%), Postives = 61/84 (72.62%), Query Frame = 0


Query: 58  MADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFG-VSSEDAKRMMDAIDKDGDGF 117
           MAD     AE +RIFK+FDANGDG+ISAAEL +AL   G V+++D KRMM  ID DGDG 
Sbjct: 1   MAD-ATEKAEHDRIFKKFDANGDGKISAAELEEALKTLGSVTADDVKRMMAEIDTDGDGN 60

Query: 118 ISFLEFADFAKDNRALMKDFAKAF 141
           IS+ EF DFA  NR LMKD AK F
Sbjct: 61  ISYQEFTDFAGANRGLMKDVAKIF 83

[Close]

BLAST of Spg038696 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 58.5 bits (140), Expect = 4.9e-09
Identity = 34/93 (36.56%), Postives = 53/93 (56.99%), Query Frame = 0


Query: 44  NRIESNQIKPPATIMADNGVNTAECERIFKRFDANGDGQISAAELGDALNGFGVS--SED 103
           N   +N+   P+T M        E +++F +FDANGDG+IS +ELG+     G S   E+
Sbjct: 5   NLESTNKSTTPSTDM--------ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEE 64

Query: 104 AKRMMDAIDKDGDGFISFLEFADFAKDNRALMK 135
             R++D ID D DGFI+  EFA   + + + ++
Sbjct: 65  LNRVLDEIDIDCDGFINQEEFATICRSSSSAVE 89

[Close]

BLAST of Spg038696 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 57.0 bits (136), Expect = 1.4e-08
Identity = 30/79 (37.97%), Postives = 47/79 (59.49%), Query Frame = 0


Query: 67  ECERIFKRFDANGDGQISAAELGDALNGFG--VSSEDAKRMMDAIDKDGDGFISFLEFAD 126
           E E +FK+FD NGDG+IS+ ELG  +   G  V  E+ ++ +  ID+ GDG+I+F EF +
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 127 F---AKDNRALMKDFAKAF 141
                 D   ++++   AF
Sbjct: 97  LNTKGMDQNDVLENLKDAF 115

[Close]

BLAST of Spg038696 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 53.1 bits (126), Expect = 2.0e-07
Identity = 30/83 (36.14%), Postives = 48/83 (57.83%), Query Frame = 0


Query: 63  VNTAECERIFKRFDANGDGQISAAELGDALNGFG--VSSEDAKRMMDAIDKDGDGFISFL 122
           ++ AE  RIF+ FD NGDG+I+  EL D+L   G  +  +D  +M++ ID +GDG++   
Sbjct: 1   MDQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIE 60

Query: 123 EFADFAK---DNRALMKDFAKAF 141
           EF    +   + R   +D  +AF
Sbjct: 61  EFGGLYQTIMEERDEEEDMREAF 83

The following BLAST results are available for this feature:

BLAST of Spg038696 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_016902062.1	9.1e-34	90.36	PREDICTED: polcalcin Bra n 2-like [Cucumis melo]	[more]
XP_022948513.1	2.0e-33	91.57	probable calcium-binding protein CML28 [Cucurbita moschata] >XP_022998721.1 prob...	[more]
XP_038874793.1	1.9e-31	86.75	probable calcium-binding protein CML28 [Benincasa hispida]	[more]
XP_004149790.1	2.5e-31	87.95	probable calcium-binding protein CML28 [Cucumis sativus] >KGN57835.1 hypothetica...	[more]
XP_023526616.1	1.8e-29	84.34	probable calcium-binding protein CML28 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg038696 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRP7	9.2e-21	66.67	Probable calcium-binding protein CML28 OS=Arabidopsis thaliana OX=3702 GN=CML28 ...	[more]
P69199	2.7e-20	64.29	Polcalcin Bra r 2 OS=Brassica campestris OX=3711 PE=1 SV=1	[more]
P69198	2.7e-20	64.29	Polcalcin Bra n 2 OS=Brassica napus OX=3708 PE=1 SV=1	[more]
Q39419	1.3e-19	62.20	Polcalcin Bet v 4 OS=Betula pendula OX=3505 GN=BETV4 PE=1 SV=1	[more]
O81092	3.9e-19	61.90	Polcalcin Ole e 3 OS=Olea europaea OX=4146 GN=OLE3 PE=1 SV=1	[more]

BLAST of Spg038696 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E1G1	4.4e-34	90.36	polcalcin Bra n 2-like OS=Cucumis melo OX=3656 GN=LOC103496835 PE=4 SV=1	[more]
A0A6J1KB11	9.8e-34	91.57	probable calcium-binding protein CML28 OS=Cucurbita maxima OX=3661 GN=LOC1114933...	[more]
A0A6J1G9I0	9.8e-34	91.57	probable calcium-binding protein CML28 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A0A0L775	1.2e-31	87.95	Putative calcium-binding protein CML28 OS=Cucumis sativus OX=3659 GN=Csa_3G34536...	[more]
A0A6J1J5E7	2.5e-29	66.07	polcalcin Syr v 3-like OS=Cucurbita maxima OX=3661 GN=LOC111481518 PE=4 SV=1	[more]

BLAST of Spg038696 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03430.1	6.5e-22	66.67	Calcium-binding EF-hand family protein	[more]
AT5G17480.1	1.8e-19	63.10	pollen calcium-binding protein 1	[more]
AT1G73630.1	4.9e-09	36.56	EF hand calcium-binding protein family	[more]
AT1G24620.1	1.4e-08	37.97	EF hand calcium-binding protein family	[more]
AT3G07490.1	2.0e-07	36.14	ARF-GAP domain 11	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 17..39
None	No IPR available	GENE3D	1.10.238.10		coord: 6..134 e-value: 2.1E-21 score: 78.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 23..45
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 23..51
None	No IPR available	PANTHER	PTHR23064:SF60	POLCALCIN NIC T 1	coord: 58..140
None	No IPR available	PANTHER	PTHR23064	TROPONIN	coord: 58..140
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 67..95 e-value: 1.1E-4 score: 31.5 coord: 101..129 e-value: 0.0043 score: 26.3
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 68..126 e-value: 3.9E-11 score: 43.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 100..132 score: 12.588623
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 63..98 score: 15.071314
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 67..127 e-value: 3.9233E-16 score: 66.0321
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 110..122
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 76..88
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 66..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg038696.1	Spg038696.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding