Homology
BLAST of Spg038507 vs. NCBI nr
Match:
XP_038905687.1 (cytokinin hydroxylase-like [Benincasa hispida])
HSP 1 Score: 781.2 bits (2016), Expect = 6.0e-222
Identity = 414/555 (74.59%), Postives = 447/555 (80.54%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
MAIVIFLTT+LVI +SSLAKIAYDT+SCYWLTPRRIRK M KQGV GP+PRPL+GNILDV
Sbjct: 1 MAIVIFLTTILVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVCGPDPRPLLGNILDV 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
AALVAKSTA DM SIDH+IV RLLPHYT WTKQY + + +
Sbjct: 61 AALVAKSTANDMKSIDHNIVGRLLPHYTTWTKQY------------------GKRFIYWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRC-TCLYGGQAQGT 180
G + R + + + E++ +K N V GK T H L +
Sbjct: 121 GTEPRLCLAETELIKEVL-SKNSN--------VCGKSWLQRQGTKHFIGKGLLMANGEDW 180
Query: 181 SFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADY 240
HI F L SYAG MVECTKKMI+S E+E DSGRTEFEIADY
Sbjct: 181 YHQRHI-VAPAFMGDKL---------KSYAGLMVECTKKMIQSLETEFDSGRTEFEIADY 240
Query: 241 MKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKS 300
MK+LTADIISRTEFD +SDKGK+IFHLLTLLQRLCAQASRHLC PGSRFFPSKYNREIKS
Sbjct: 241 MKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQASRHLCLPGSRFFPSKYNREIKS 300
Query: 301 LKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED-GFSLNLQLIMDEC 360
LKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED GFSLNLQLIMDEC
Sbjct: 301 LKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDNGFSLNLQLIMDEC 360
Query: 361 KTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMV 420
KTFFFAGHETTALLLTWTIMLLATNP+WQHKVR E+NQVCN G +PS+EHLSKLTLLNMV
Sbjct: 361 KTFFFAGHETTALLLTWTIMLLATNPDWQHKVRHEVNQVCNNGGSPSVEHLSKLTLLNMV 420
Query: 421 INESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPER 480
INESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWIPVLAIHHSEELWG+DANEFKPER
Sbjct: 421 INESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGEDANEFKPER 480
Query: 481 FATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTI 540
FA+KPFSGGRFIPFA+GPRNCIGQ FA+MEAKIILAM +SKFSFTISDSYRHAPV VLTI
Sbjct: 481 FASKPFSGGRFIPFASGPRNCIGQGFALMEAKIILAMFISKFSFTISDSYRHAPVSVLTI 518
Query: 541 QPKYGVQVCLTPLNS 554
QPKYGVQV LTP+NS
Sbjct: 541 QPKYGVQVYLTPINS 518
BLAST of Spg038507 vs. NCBI nr
Match:
XP_008461587.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])
HSP 1 Score: 778.5 bits (2009), Expect = 3.9e-221
Identity = 407/556 (73.20%), Postives = 444/556 (79.86%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
MAI+IFLTTLLVI +SSLAKIAYDT+SCYWLTPRRIRK M KQGVRGP PRPL+GNILDV
Sbjct: 1 MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
AAL+AKSTA DMSSIDH+IVDRLLPHYT WTKQY + + +
Sbjct: 61 AALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQY------------------GKRFIYWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
G++ R + + + E+++ + + W + + L +
Sbjct: 121 GMEPRLCLAETELIKEVLSKNS--------NVCGRSWLQRQGTKHFIGKGLLMANGEDWY 180
Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
HI F L SYAG MVECT K+I+S E+ELDSGRTEFEIADYM
Sbjct: 181 HQRHI-VAPSFMGDKL---------KSYAGLMVECTDKLIQSLETELDSGRTEFEIADYM 240
Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
K+LTADIISRTEFD +SDKG QIFHLLTLLQ LCAQASRHLC PGSRFFPSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSL 300
Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED---GFSLNLQLIMDE 360
KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRK++D GFSLNLQLIMDE
Sbjct: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKKDDGDNGFSLNLQLIMDE 360
Query: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNM 420
CKTFFFAGHETTALLLTWTIMLLATNPNWQ KVR E+NQVC+ G TPS+EHLSK TLLNM
Sbjct: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQVCHNGATPSVEHLSKFTLLNM 420
Query: 421 VINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPE 480
VINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWIPVLAIHHSEELWGKD NEFKPE
Sbjct: 421 VINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPE 480
Query: 481 RFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLT 540
RFA+KPFSGGRFIPFAAGPRNC+GQ FA+MEAKIILAMLLSKFSFTISDSYRHAPV VLT
Sbjct: 481 RFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIILAMLLSKFSFTISDSYRHAPVSVLT 520
Query: 541 IQPKYGVQVCLTPLNS 554
IQPKYGVQV LTP+NS
Sbjct: 541 IQPKYGVQVYLTPINS 520
BLAST of Spg038507 vs. NCBI nr
Match:
XP_004145660.1 (cytokinin hydroxylase [Cucumis sativus] >KGN57750.1 hypothetical protein Csa_009800 [Cucumis sativus])
HSP 1 Score: 771.2 bits (1990), Expect = 6.2e-219
Identity = 404/556 (72.66%), Postives = 441/556 (79.32%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
MAI+I LTTLLVI +SSLAKIAYDT+SCYWLTPRRIRK M KQGVRGP PRPL+GNILDV
Sbjct: 1 MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
AAL+ KSTA DMSSIDH+IVDRLLPHYT WTKQY + + +
Sbjct: 61 AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQY------------------GKRFIYWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
G++ R + + + E+++ + + W + + L +
Sbjct: 121 GMEPRLCLAETELIKEVLSKNS--------NVCGRSWLQRQGTKHFIGKGLLMANGEDWY 180
Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
HI F L SYAG MVECT K+I+S E+E DSGRTEFEIADYM
Sbjct: 181 HQRHI-VAPSFMGDKL---------KSYAGLMVECTNKLIQSLETEFDSGRTEFEIADYM 240
Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
K+LTADIISRTEFD +SDKG QIFHLLTLLQ LCAQASRHLC PGSRFFPSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSL 300
Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED---GFSLNLQLIMDE 360
KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQ++RED GFSLNLQLIMDE
Sbjct: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQKRREDGDNGFSLNLQLIMDE 360
Query: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNM 420
CKTFFFAGHETTALLLTWTIMLLATNPNWQ KVR E+NQVC+ G TPS+EHLSK TLLNM
Sbjct: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQVCHNGATPSVEHLSKFTLLNM 420
Query: 421 VINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPE 480
VINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWIPVLAIHHSEELWGKD NEFKPE
Sbjct: 421 VINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPE 480
Query: 481 RFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLT 540
RFA+KPFSGGRFIPFAAGPRNCIGQ FA+MEAKIILAMLLSKFSF+ISDSYRHAPV VLT
Sbjct: 481 RFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIILAMLLSKFSFSISDSYRHAPVSVLT 520
Query: 541 IQPKYGVQVCLTPLNS 554
IQPKYGVQV LTP+NS
Sbjct: 541 IQPKYGVQVYLTPINS 520
BLAST of Spg038507 vs. NCBI nr
Match:
XP_022983651.1 (cytokinin hydroxylase-like [Cucurbita maxima])
HSP 1 Score: 731.1 bits (1886), Expect = 7.1e-207
Identity = 388/554 (70.04%), Postives = 426/554 (76.90%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
MA +IFLTTL VI +SSLAKI+YDT S Y LTPRRIRKTM +QGVRGP+PR LIGNILDV
Sbjct: 1 MAFIIFLTTLFVIFISSLAKISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
LVAKSTA DMSSIDHDIVDRLLPHYT WTKQY + + +
Sbjct: 61 VGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQY------------------GKRFIYWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
G + R + + + E++ K+ + W + + L +
Sbjct: 121 GTEPRLCLAETELIKELLGKKSS--------VCGRSWLQRQGTKHFIGKGLLMANGEDWY 180
Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
HI F L SYAG+MVECT KMI+S E+ELDSGRTEFEIADYM
Sbjct: 181 HQRHI-VAPAFMGDKL---------KSYAGFMVECTNKMIQSMETELDSGRTEFEIADYM 240
Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
K+LTADIISRTEFDT SDKG QIFHLLTLLQRLCAQASRHLC PGSRF PSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSKYNREIKSL 300
Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM-QRKREDGFSLNLQLIMDECK 360
K EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLLNEM QRK +LNLQLI+DECK
Sbjct: 301 KKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQRKNSTTTTLNLQLIIDECK 360
Query: 361 TFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVI 420
TFFFAGHETTALLLTWTIMLLATNPNWQH VR E+NQVCN TPSI+HLSKLTLLNMVI
Sbjct: 361 TFFFAGHETTALLLTWTIMLLATNPNWQHMVRAEVNQVCN-ARTPSIQHLSKLTLLNMVI 420
Query: 421 NESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERF 480
NESLRLYPPATVLPRMA ED+KLGD++IPKGLSIWIPVLAIHHSEELWGKDAN+F P RF
Sbjct: 421 NESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGKDANQFNPNRF 480
Query: 481 ATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTIQ 540
A K F GGRFIPFA GPRNCIGQ FA+MEAKIILAM LSKFSFTISD+YRHAPV+VLTIQ
Sbjct: 481 AAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKFSFTISDTYRHAPVVVLTIQ 517
Query: 541 PKYGVQVCLTPLNS 554
PK+GVQVCLTP +S
Sbjct: 541 PKHGVQVCLTPFSS 517
BLAST of Spg038507 vs. NCBI nr
Match:
XP_023528555.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 726.1 bits (1873), Expect = 2.3e-205
Identity = 383/557 (68.76%), Postives = 427/557 (76.66%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
MA +IFLTTLL+I ++SLA+I+YDT S Y LTPRRIRKTM +QGVRGP+PR LIGNILDV
Sbjct: 1 MAFIIFLTTLLLIFIASLARISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
A LVAKSTA DMSSIDHDIVDRLLPHYT WTKQY + + +
Sbjct: 61 AGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQY------------------GKRFIYWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
G + R + + + E++ K+ + W + + L +
Sbjct: 121 GTEPRLCLAETELIKELLGKKSS--------VCGRSWLQRQGTKHFIGKGLLMANGEDWY 180
Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
HI F L SYAG+MVECT KMI+S E+EL+SGRTEFEIADYM
Sbjct: 181 HQRHI-VAPAFMGDKL---------KSYAGFMVECTNKMIQSMETELESGRTEFEIADYM 240
Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
K+LTADIISRTEFDT SDKG QIFHLLTLLQRLCAQASRHLC PGSRF PSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSKYNREIKSL 300
Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED----GFSLNLQLIMD 360
K EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLLNE+Q+++ +LNLQLIMD
Sbjct: 301 KKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNELQQRKNSSTTTSTTLNLQLIMD 360
Query: 361 ECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLN 420
ECKTFFFAGHETTALLLTWTIMLLATNPNWQH VR ++NQVCN TPSI+HLSKLTLLN
Sbjct: 361 ECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN-ARTPSIQHLSKLTLLN 420
Query: 421 MVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKP 480
MVINESLRLYPPATVLPRMA ED+KLGD++IPKGLSIWIPVLAIHHSEELWGKDAN+F P
Sbjct: 421 MVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGKDANQFNP 480
Query: 481 ERFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVL 540
RF K F GGRFIPFA GPRNCIGQ FA+MEAKIILAMLLSKF FTISD+YRHAPV+VL
Sbjct: 481 NRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMLLSKFRFTISDTYRHAPVVVL 520
Query: 541 TIQPKYGVQVCLTPLNS 554
TIQPKYGVQVCLTP S
Sbjct: 541 TIQPKYGVQVCLTPFTS 520
BLAST of Spg038507 vs. ExPASy Swiss-Prot
Match:
Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)
HSP 1 Score: 601.3 bits (1549), Expect = 1.1e-170
Identity = 318/554 (57.40%), Postives = 393/554 (70.94%), Query Frame = 0
Query: 3 IVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDVAA 62
++ L +LLVI V+++ ++ YDT+SCYWLTPRRI+K M +QGV GP+PRPL GNIL+++A
Sbjct: 2 LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISA 61
Query: 63 LVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGDGI 122
+V++S + D SI HDIV RLLPHY AW+KQY + + +G
Sbjct: 62 MVSQSASKDCDSIHHDIVGRLLPHYVAWSKQY------------------GKRFIVWNGT 121
Query: 123 DKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGK-WGRLVSSTSHRCTCLYGGQAQGTSF 182
D R + + + E++ G V G+ W + + + L Q
Sbjct: 122 DPRLCLTETELIKELLMKHNG---------VSGRSWLQQQGTKNFIGRGLLMANGQDWHH 181
Query: 183 PPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYMK 242
H++ F+ L YA +MVECT K++E E+ G E EI + M
Sbjct: 182 QRHLA-APAFTGERL---------KGYARHMVECTSKLVERLRKEVGEGANEVEIGEEMH 241
Query: 243 RLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSLK 302
+LTADIISRT+F +S +KGK++F+ LT+LQR CAQA+RHLCFPGSRF PSKYNREIKSLK
Sbjct: 242 KLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLCFPGSRFLPSKYNREIKSLK 301
Query: 303 MEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQ-RKREDGFSLNLQLIMDECKT 362
EVERLL+EIIQSR+D E+GRS+ +G DLLG+LLNEM K + + NLQLIMDECKT
Sbjct: 302 KEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKT 361
Query: 363 FFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVIN 422
FFFAGHETTALLLTWT MLLA NP WQ KVR+E+ +V PS++ LSKLT L+ VIN
Sbjct: 362 FFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVIN 421
Query: 423 ESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERFA 482
ESLRLYPPAT+LPRMAFED+KLGDL IPKGLSIWIPVLAIHHSEELWGKDAN+F PERF
Sbjct: 422 ESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERFG 481
Query: 483 TKPFSGGR-FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTIQ 542
+PF+ GR FIPFAAGPRNCIGQ FA+MEAKIILA L+SKF+FTIS +YRHAP++VLTI+
Sbjct: 482 GRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIK 518
Query: 543 PKYGVQVCLTPLNS 554
PKYGVQV L PL S
Sbjct: 542 PKYGVQVILKPLVS 518
BLAST of Spg038507 vs. ExPASy Swiss-Prot
Match:
Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)
HSP 1 Score: 587.4 bits (1513), Expect = 1.7e-166
Identity = 315/554 (56.86%), Postives = 387/554 (69.86%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
M + + L +LVI+++ + ++ YD++ CY+LTPRRI+K M +QG+ GP+PR L GNI+D+
Sbjct: 1 MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
+ +++ S + D SSI H+IV RLLPHY +W+KQY + + +
Sbjct: 61 SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQY------------------GKRFIMWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGK-WGRLVSSTSHRCTCLYGGQAQGT 180
G + R C+ E K + L + V GK W + + L +
Sbjct: 121 GTEPRL------CLTETEMIK---ELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAW 180
Query: 181 SFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADY 240
H++ F+ L YA +MVECTK M E E+ E EI +
Sbjct: 181 HHQRHMA-APAFTRDRL---------KGYAKHMVECTKMMAERLRKEVGE---EVEIGEE 240
Query: 241 MKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKS 300
M+RLTADIISRTEF +S DKGK++F LLT+LQRLCAQA+RHLCFPGSRF PSKYNREIKS
Sbjct: 241 MRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPGSRFLPSKYNREIKS 300
Query: 301 LKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLNLQLIMDECK 360
LK EVERLLMEII SRKD VEIGRS+ YG DLLG+LLN+M + +LN+Q+IMDECK
Sbjct: 301 LKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNKN---NLNVQMIMDECK 360
Query: 361 TFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVI 420
TFFF GHETT+LLLTWT+MLLA NP WQ VRDE+ QVC PS+E LS LT LN VI
Sbjct: 361 TFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVI 420
Query: 421 NESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERF 480
NESLRLYPPAT+LPRMAFEDIKLGDL IPKGLSIWIPVLAIHHS ELWG+DANEF PERF
Sbjct: 421 NESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERF 480
Query: 481 ATKPFSGGR-FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTI 540
T+ F+ R F+PFAAGPRNCIGQ FAMMEAKIILAML+SKFSF IS++YRHAP++VLTI
Sbjct: 481 TTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTI 511
Query: 541 QPKYGVQVCLTPLN 553
+PKYGVQ+ L PL+
Sbjct: 541 KPKYGVQLVLKPLD 511
BLAST of Spg038507 vs. ExPASy Swiss-Prot
Match:
Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)
HSP 1 Score: 288.5 bits (737), Expect = 1.6e-76
Identity = 193/566 (34.10%), Postives = 289/566 (51.06%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
M +VIFL +++IL+ L +I + W P + + + QG+ GP R GN+ ++
Sbjct: 1 MGLVIFLALIVLILIIGL-RIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSEI 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
+ +S + +DI R+LPHY W QY E L +
Sbjct: 61 KKMKRESHLSILDPSSNDIFPRILPHYQKWMSQY------------------GETFLYWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSH-RCTCLYGGQA--- 180
G + R C+ + AKT ++ K G V S + L G +
Sbjct: 121 GTEPRI------CISDPELAKT---------MLSNKLGFFVKSKARPEAVKLVGSKGLVF 180
Query: 181 -QGTSFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEF- 240
+G + H L S L MV+CT KM+E + E TE
Sbjct: 181 IEGADWVRHRRILNPAFSIDRLKI--------MTTVMVDCTLKMLEEWRKESTKEETEHP 240
Query: 241 ----EIADYMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFP 300
E+ + +RLTADII+ + F +S +G ++F L+R + + PG+++ P
Sbjct: 241 KIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELKRCYTTSLNQVSIPGTQYLP 300
Query: 301 SKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLN 360
+ N + L+ +++ + II SR + + YG DLLG+LL + ++ ++
Sbjct: 301 TPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDDLLGILLKAYNTEGKER-KMS 360
Query: 361 LQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLS 420
++ I+ EC+TFFF GHETT+ LL WT MLL+ + +WQ K+R+E+ + C TP E S
Sbjct: 361 IEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECGKEKTPDSETFS 420
Query: 421 KLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKD 480
KL L+NMVI ESLRLY P + L R A +IKLGDLEIPKG ++ IP+L +H + LWG D
Sbjct: 421 KLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPKGTTVVIPLLKMHSDKTLWGSD 480
Query: 481 ANEFKPERFATKPFSGGR----FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSF-TIS 540
A++F P RFA + F+ GPR CIGQ F M+EAK +L M+L +F F ++
Sbjct: 481 ADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFVMIEAKTVLTMILQRFRFISLC 517
Query: 541 DSYRHAPVIVLTIQPKYGVQVCLTPL 552
D Y+H PV +TIQP+YG+ V L PL
Sbjct: 541 DEYKHTPVDNVTIQPQYGLPVMLQPL 517
BLAST of Spg038507 vs. ExPASy Swiss-Prot
Match:
Q6Z6D6 (Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 SV=1)
HSP 1 Score: 280.4 bits (716), Expect = 4.4e-74
Identity = 183/566 (32.33%), Postives = 272/566 (48.06%), Query Frame = 0
Query: 30 WLTPRRIRKTMAKQGVRGPEPRPLIGNILDVAALVAKSTAGDMS-SIDHDIVDRLLPHYT 89
W PRR+ A QGVRGP R L+G++ ++ AL+A++++ MS H+ + R+L Y
Sbjct: 42 WWRPRRLEAHFAAQGVRGPPYRFLLGSVREMVALMAEASSKPMSPPTSHNALPRVLAFYH 101
Query: 90 AWTKQYA------FG--------SDRYRKEVHLLERDGAEAV--------LDGDGIDKRT 149
W K Y FG +E+ L D + L+GDG
Sbjct: 102 YWRKIYGHRFLIWFGPTPRLTVAEPELIREIFLTRADAFDRYEAHPVVRQLEGDG----- 161
Query: 150 IGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTSFPPHIS 209
+V+ LHR + D +P +++
Sbjct: 162 ----------LVSLHGDKWALHRRVLTD------------------------AFYPDNLN 221
Query: 210 YLF--VFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYMKRLT 269
L V S + L+A + G M E SG E ++A++ + +T
Sbjct: 222 RLIPHVGKSVAALAAKW--------GAMAEAG-----------GSGEVEVDVAEWFQAVT 281
Query: 270 ADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSLKMEV 329
+ I+R F S D G+ +F + L ++A R + PG RF P+K NR L E+
Sbjct: 282 EEAITRATFGRSYDDGRVVFAMQGQLMAFASEAFRKVLVPGYRFLPTKKNRLSWRLDREI 341
Query: 330 ERLLMEIIQSRKDGVEIGRSTCYGS--DLLGMLLNEMQ----------RKREDGFSLNLQ 389
R LM +I R D E G GS DLLG+++N K ++ ++
Sbjct: 342 RRSLMRLIGRRSDEAEQGEKADDGSFRDLLGLMINAGAAAATRGNAGGEKNSPAAAIPVE 401
Query: 390 LIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKL 449
+++ECKTFFFAG +TT LLTW +LLA +P+WQ + R E+ VC G PS EHL KL
Sbjct: 402 DMLEECKTFFFAGKQTTTNLLTWATVLLAMHPDWQERARREVFDVCGAGELPSKEHLPKL 461
Query: 450 TLLNMVINESLRLYPPATVLPRMAFEDIKLGD-LEIPKGLSIWIPVLAIHHSEELWGKDA 509
L M++NE+LRLYPPA R A D++L D IP+ + + +P++AIHH WG DA
Sbjct: 462 KTLGMIMNETLRLYPPAVATIRRAKVDVQLSDGCMIPRDMELLVPIMAIHHDTRYWGPDA 521
Query: 510 NEFKPERFATKPFSGGR----FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDS 554
++F P RFA + FIPF G R C+GQ A +EAK+ +A+LL +F S +
Sbjct: 522 SQFNPARFANGASKAAKHPLAFIPFGLGSRMCVGQNLARLEAKLTMAILLQRFEIRTSPN 549
BLAST of Spg038507 vs. ExPASy Swiss-Prot
Match:
F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)
HSP 1 Score: 280.0 bits (715), Expect = 5.7e-74
Identity = 194/562 (34.52%), Postives = 277/562 (49.29%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
M ++ + L + LV + Y P + + KQG+ GP+ R L GN+ ++
Sbjct: 56 MELLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREI 115
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
+ ++ + +DIV R+LPH W QY E L
Sbjct: 116 RKMKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQY------------------GETFLYWQ 175
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQ---AQ 180
G D R C+ + AK Q L + + K S T L G
Sbjct: 176 GTDPRL------CISDHELAK---QILSNKFVFFSK-----SKTKPEILKLSGNGLIFVN 235
Query: 181 GTSFPPHISYLF-VFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTE--F 240
G + H L FS L MV+CT +M ++ + + TE
Sbjct: 236 GLDWVRHRRILNPAFSMDKL---------KLMTQLMVDCTFRMFLEWKKQRNGVETEQFV 295
Query: 241 EIADYMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYN 300
I+ KRLTADII+ F +S +G ++F LQ+ CA A L FPG ++ P+ N
Sbjct: 296 LISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSN 355
Query: 301 REIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLNLQLI 360
+I L M+V + II +R S YG+DLLG++L E +++ I
Sbjct: 356 LQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIMLTAAS-SNESEKKMSIDEI 415
Query: 361 MDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTL 420
++ECKTFFFAGHETTA LLTW+ MLL+ + +WQ K+R+E+ C P E SKL L
Sbjct: 416 IEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKL 475
Query: 421 LNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEF 480
+N V ESLRLY P L R+A ED+KLG+LEIPKG +I +P+ +H + +WG DA++F
Sbjct: 476 MNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDADKF 535
Query: 481 KPERFATKPFSGGR----FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRH 540
P RFA + F+ GPR CIGQ FA+MEAK +LAM+L +F +S Y+H
Sbjct: 536 NPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADYKH 571
Query: 541 APVIVLTIQPKYGVQVCLTPLN 553
AP LT+QP+Y + V L P++
Sbjct: 596 APADHLTLQPQYDLPVILEPID 571
BLAST of Spg038507 vs. ExPASy TrEMBL
Match:
A0A1S3CGC0 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1)
HSP 1 Score: 778.5 bits (2009), Expect = 1.9e-221
Identity = 407/556 (73.20%), Postives = 444/556 (79.86%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
MAI+IFLTTLLVI +SSLAKIAYDT+SCYWLTPRRIRK M KQGVRGP PRPL+GNILDV
Sbjct: 1 MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
AAL+AKSTA DMSSIDH+IVDRLLPHYT WTKQY + + +
Sbjct: 61 AALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQY------------------GKRFIYWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
G++ R + + + E+++ + + W + + L +
Sbjct: 121 GMEPRLCLAETELIKEVLSKNS--------NVCGRSWLQRQGTKHFIGKGLLMANGEDWY 180
Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
HI F L SYAG MVECT K+I+S E+ELDSGRTEFEIADYM
Sbjct: 181 HQRHI-VAPSFMGDKL---------KSYAGLMVECTDKLIQSLETELDSGRTEFEIADYM 240
Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
K+LTADIISRTEFD +SDKG QIFHLLTLLQ LCAQASRHLC PGSRFFPSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSL 300
Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED---GFSLNLQLIMDE 360
KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRK++D GFSLNLQLIMDE
Sbjct: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKKDDGDNGFSLNLQLIMDE 360
Query: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNM 420
CKTFFFAGHETTALLLTWTIMLLATNPNWQ KVR E+NQVC+ G TPS+EHLSK TLLNM
Sbjct: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQVCHNGATPSVEHLSKFTLLNM 420
Query: 421 VINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPE 480
VINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWIPVLAIHHSEELWGKD NEFKPE
Sbjct: 421 VINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPE 480
Query: 481 RFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLT 540
RFA+KPFSGGRFIPFAAGPRNC+GQ FA+MEAKIILAMLLSKFSFTISDSYRHAPV VLT
Sbjct: 481 RFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIILAMLLSKFSFTISDSYRHAPVSVLT 520
Query: 541 IQPKYGVQVCLTPLNS 554
IQPKYGVQV LTP+NS
Sbjct: 541 IQPKYGVQVYLTPINS 520
BLAST of Spg038507 vs. ExPASy TrEMBL
Match:
A0A0A0L6Y6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1)
HSP 1 Score: 771.2 bits (1990), Expect = 3.0e-219
Identity = 404/556 (72.66%), Postives = 441/556 (79.32%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
MAI+I LTTLLVI +SSLAKIAYDT+SCYWLTPRRIRK M KQGVRGP PRPL+GNILDV
Sbjct: 1 MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
AAL+ KSTA DMSSIDH+IVDRLLPHYT WTKQY + + +
Sbjct: 61 AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQY------------------GKRFIYWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
G++ R + + + E+++ + + W + + L +
Sbjct: 121 GMEPRLCLAETELIKEVLSKNS--------NVCGRSWLQRQGTKHFIGKGLLMANGEDWY 180
Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
HI F L SYAG MVECT K+I+S E+E DSGRTEFEIADYM
Sbjct: 181 HQRHI-VAPSFMGDKL---------KSYAGLMVECTNKLIQSLETEFDSGRTEFEIADYM 240
Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
K+LTADIISRTEFD +SDKG QIFHLLTLLQ LCAQASRHLC PGSRFFPSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSL 300
Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED---GFSLNLQLIMDE 360
KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQ++RED GFSLNLQLIMDE
Sbjct: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQKRREDGDNGFSLNLQLIMDE 360
Query: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNM 420
CKTFFFAGHETTALLLTWTIMLLATNPNWQ KVR E+NQVC+ G TPS+EHLSK TLLNM
Sbjct: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQVCHNGATPSVEHLSKFTLLNM 420
Query: 421 VINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPE 480
VINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWIPVLAIHHSEELWGKD NEFKPE
Sbjct: 421 VINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPE 480
Query: 481 RFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLT 540
RFA+KPFSGGRFIPFAAGPRNCIGQ FA+MEAKIILAMLLSKFSF+ISDSYRHAPV VLT
Sbjct: 481 RFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIILAMLLSKFSFSISDSYRHAPVSVLT 520
Query: 541 IQPKYGVQVCLTPLNS 554
IQPKYGVQV LTP+NS
Sbjct: 541 IQPKYGVQVYLTPINS 520
BLAST of Spg038507 vs. ExPASy TrEMBL
Match:
A0A6J1J2Y1 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1)
HSP 1 Score: 731.1 bits (1886), Expect = 3.5e-207
Identity = 388/554 (70.04%), Postives = 426/554 (76.90%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
MA +IFLTTL VI +SSLAKI+YDT S Y LTPRRIRKTM +QGVRGP+PR LIGNILDV
Sbjct: 1 MAFIIFLTTLFVIFISSLAKISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
LVAKSTA DMSSIDHDIVDRLLPHYT WTKQY + + +
Sbjct: 61 VGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQY------------------GKRFIYWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
G + R + + + E++ K+ + W + + L +
Sbjct: 121 GTEPRLCLAETELIKELLGKKSS--------VCGRSWLQRQGTKHFIGKGLLMANGEDWY 180
Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
HI F L SYAG+MVECT KMI+S E+ELDSGRTEFEIADYM
Sbjct: 181 HQRHI-VAPAFMGDKL---------KSYAGFMVECTNKMIQSMETELDSGRTEFEIADYM 240
Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
K+LTADIISRTEFDT SDKG QIFHLLTLLQRLCAQASRHLC PGSRF PSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSKYNREIKSL 300
Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM-QRKREDGFSLNLQLIMDECK 360
K EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLLNEM QRK +LNLQLI+DECK
Sbjct: 301 KKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQRKNSTTTTLNLQLIIDECK 360
Query: 361 TFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVI 420
TFFFAGHETTALLLTWTIMLLATNPNWQH VR E+NQVCN TPSI+HLSKLTLLNMVI
Sbjct: 361 TFFFAGHETTALLLTWTIMLLATNPNWQHMVRAEVNQVCN-ARTPSIQHLSKLTLLNMVI 420
Query: 421 NESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERF 480
NESLRLYPPATVLPRMA ED+KLGD++IPKGLSIWIPVLAIHHSEELWGKDAN+F P RF
Sbjct: 421 NESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGKDANQFNPNRF 480
Query: 481 ATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTIQ 540
A K F GGRFIPFA GPRNCIGQ FA+MEAKIILAM LSKFSFTISD+YRHAPV+VLTIQ
Sbjct: 481 AAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKFSFTISDTYRHAPVVVLTIQ 517
Query: 541 PKYGVQVCLTPLNS 554
PK+GVQVCLTP +S
Sbjct: 541 PKHGVQVCLTPFSS 517
BLAST of Spg038507 vs. ExPASy TrEMBL
Match:
A0A6J1F8S1 (cytokinin hydroxylase OS=Cucurbita moschata OX=3662 GN=LOC111441866 PE=3 SV=1)
HSP 1 Score: 724.9 bits (1870), Expect = 2.5e-205
Identity = 381/553 (68.90%), Postives = 424/553 (76.67%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
MA +IFLTTLL+I ++SLA+I+YDT S Y LTPRRIRKTM +QGVRGP+PR LIGNILDV
Sbjct: 1 MAFIIFLTTLLLIFLASLARISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
A LVAKSTA MSSIDHDIVDRLLPHYT WTKQY + + +
Sbjct: 61 AGLVAKSTANHMSSIDHDIVDRLLPHYTIWTKQY------------------GKRFIYWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
G + R + + + E++ K+ + W + + L +
Sbjct: 121 GTEPRLCLAETELIKELLGKKSS--------VCGRSWLQRQGTKHFIGKGLLMANGEDWY 180
Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
HI F L SYAG+MVECT KMI+S E+EL+SGRTEFEIADYM
Sbjct: 181 HQRHI-MAPAFMGDKL---------KSYAGFMVECTNKMIQSMETELESGRTEFEIADYM 240
Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
K+LTADIISRTEFDT SDKG QIFHLLTLLQRLCAQASRHLC PGSRF PSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSKYNREIKSL 300
Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLNLQLIMDECKT 360
K EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLLNEMQ+++ +LNLQLIMDECKT
Sbjct: 301 KKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQRKNISTTLNLQLIMDECKT 360
Query: 361 FFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVIN 420
FFFAGHETTALLLTWTIMLLATNPNWQH VR ++NQVCN PSI+HLSKLTLLNMVIN
Sbjct: 361 FFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN-ARAPSIQHLSKLTLLNMVIN 420
Query: 421 ESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERFA 480
ESLRLYPPATVLPRMA ED+KLGD++IPKGLSIWIPVLAIHHSEELWGKDAN+F P RF
Sbjct: 421 ESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGKDANQFNPNRFG 480
Query: 481 TKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTIQP 540
K F GGRFIPFA GPRNCIGQ FA+MEAKIILAM LSKF FTISD+YRHAPV+VLTIQP
Sbjct: 481 AKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKFRFTISDTYRHAPVVVLTIQP 516
Query: 541 KYGVQVCLTPLNS 554
KYGVQVCLTP S
Sbjct: 541 KYGVQVCLTPFTS 516
BLAST of Spg038507 vs. ExPASy TrEMBL
Match:
A0A5A7V8T6 (Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold508G00410 PE=3 SV=1)
HSP 1 Score: 716.5 bits (1848), Expect = 8.8e-203
Identity = 373/517 (72.15%), Postives = 407/517 (78.72%), Query Frame = 0
Query: 40 MAKQGVRGPEPRPLIGNILDVAALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSD 99
M KQGVRGP PRPL+GNILDVAAL+AKSTA DMSSIDH+IVDRLLPHYT WTKQY
Sbjct: 1 MEKQGVRGPVPRPLVGNILDVAALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQY----- 60
Query: 100 RYRKEVHLLERDGAEAVLDGDGIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGR 159
+ + +G++ R + + + E+++ + + W +
Sbjct: 61 -------------GKRFIYWNGMEPRLCLAETELIKEVLSKNS--------NVCGRSWLQ 120
Query: 160 LVSSTSHRCTCLYGGQAQGTSFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKM 219
+ L + HI F L SYAG MVECT K+
Sbjct: 121 RQGTKHFIGKGLLMANGEDWYHQRHI-VAPSFMGDKL---------KSYAGLMVECTDKL 180
Query: 220 IESFESELDSGRTEFEIADYMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASR 279
I+S E+ELDSGRTEFEIADYMK+LTADIISRTEFD +SDKG QIFHLLTLLQ LCAQASR
Sbjct: 181 IQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQASR 240
Query: 280 HLCFPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM 339
HLC PGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM
Sbjct: 241 HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM 300
Query: 340 QRKRED---GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQ 399
QRK++D GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQ KVR E+NQ
Sbjct: 301 QRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQ 360
Query: 400 VCNGGTTPSIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIP 459
VC+ G TPS+EHLSK TLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWIP
Sbjct: 361 VCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIP 420
Query: 460 VLAIHHSEELWGKDANEFKPERFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAML 519
VLAIHHSEELWGKD NEFKPERFA+KPFSGGRFIPFAAGPRNC+GQ FA+MEAKIILAML
Sbjct: 421 VLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIILAML 480
Query: 520 LSKFSFTISDSYRHAPVIVLTIQPKYGVQVCLTPLNS 554
LSKFSFTISDSYRHAPV VLTIQPKYGVQV LTP+NS
Sbjct: 481 LSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 481
BLAST of Spg038507 vs. TAIR 10
Match:
AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )
HSP 1 Score: 601.3 bits (1549), Expect = 7.9e-172
Identity = 318/554 (57.40%), Postives = 393/554 (70.94%), Query Frame = 0
Query: 3 IVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDVAA 62
++ L +LLVI V+++ ++ YDT+SCYWLTPRRI+K M +QGV GP+PRPL GNIL+++A
Sbjct: 2 LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISA 61
Query: 63 LVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGDGI 122
+V++S + D SI HDIV RLLPHY AW+KQY + + +G
Sbjct: 62 MVSQSASKDCDSIHHDIVGRLLPHYVAWSKQY------------------GKRFIVWNGT 121
Query: 123 DKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGK-WGRLVSSTSHRCTCLYGGQAQGTSF 182
D R + + + E++ G V G+ W + + + L Q
Sbjct: 122 DPRLCLTETELIKELLMKHNG---------VSGRSWLQQQGTKNFIGRGLLMANGQDWHH 181
Query: 183 PPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYMK 242
H++ F+ L YA +MVECT K++E E+ G E EI + M
Sbjct: 182 QRHLA-APAFTGERL---------KGYARHMVECTSKLVERLRKEVGEGANEVEIGEEMH 241
Query: 243 RLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSLK 302
+LTADIISRT+F +S +KGK++F+ LT+LQR CAQA+RHLCFPGSRF PSKYNREIKSLK
Sbjct: 242 KLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLCFPGSRFLPSKYNREIKSLK 301
Query: 303 MEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQ-RKREDGFSLNLQLIMDECKT 362
EVERLL+EIIQSR+D E+GRS+ +G DLLG+LLNEM K + + NLQLIMDECKT
Sbjct: 302 KEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKT 361
Query: 363 FFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVIN 422
FFFAGHETTALLLTWT MLLA NP WQ KVR+E+ +V PS++ LSKLT L+ VIN
Sbjct: 362 FFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVIN 421
Query: 423 ESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERFA 482
ESLRLYPPAT+LPRMAFED+KLGDL IPKGLSIWIPVLAIHHSEELWGKDAN+F PERF
Sbjct: 422 ESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERFG 481
Query: 483 TKPFSGGR-FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTIQ 542
+PF+ GR FIPFAAGPRNCIGQ FA+MEAKIILA L+SKF+FTIS +YRHAP++VLTI+
Sbjct: 482 GRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIK 518
Query: 543 PKYGVQVCLTPLNS 554
PKYGVQV L PL S
Sbjct: 542 PKYGVQVILKPLVS 518
BLAST of Spg038507 vs. TAIR 10
Match:
AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )
HSP 1 Score: 587.4 bits (1513), Expect = 1.2e-167
Identity = 315/554 (56.86%), Postives = 387/554 (69.86%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
M + + L +LVI+++ + ++ YD++ CY+LTPRRI+K M +QG+ GP+PR L GNI+D+
Sbjct: 1 MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
+ +++ S + D SSI H+IV RLLPHY +W+KQY + + +
Sbjct: 61 SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQY------------------GKRFIMWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGK-WGRLVSSTSHRCTCLYGGQAQGT 180
G + R C+ E K + L + V GK W + + L +
Sbjct: 121 GTEPRL------CLTETEMIK---ELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAW 180
Query: 181 SFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADY 240
H++ F+ L YA +MVECTK M E E+ E EI +
Sbjct: 181 HHQRHMA-APAFTRDRL---------KGYAKHMVECTKMMAERLRKEVGE---EVEIGEE 240
Query: 241 MKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKS 300
M+RLTADIISRTEF +S DKGK++F LLT+LQRLCAQA+RHLCFPGSRF PSKYNREIKS
Sbjct: 241 MRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPGSRFLPSKYNREIKS 300
Query: 301 LKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLNLQLIMDECK 360
LK EVERLLMEII SRKD VEIGRS+ YG DLLG+LLN+M + +LN+Q+IMDECK
Sbjct: 301 LKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNKN---NLNVQMIMDECK 360
Query: 361 TFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVI 420
TFFF GHETT+LLLTWT+MLLA NP WQ VRDE+ QVC PS+E LS LT LN VI
Sbjct: 361 TFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVI 420
Query: 421 NESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERF 480
NESLRLYPPAT+LPRMAFEDIKLGDL IPKGLSIWIPVLAIHHS ELWG+DANEF PERF
Sbjct: 421 NESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERF 480
Query: 481 ATKPFSGGR-FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTI 540
T+ F+ R F+PFAAGPRNCIGQ FAMMEAKIILAML+SKFSF IS++YRHAP++VLTI
Sbjct: 481 TTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTI 511
Query: 541 QPKYGVQVCLTPLN 553
+PKYGVQ+ L PL+
Sbjct: 541 KPKYGVQLVLKPLD 511
BLAST of Spg038507 vs. TAIR 10
Match:
AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )
HSP 1 Score: 288.5 bits (737), Expect = 1.1e-77
Identity = 193/566 (34.10%), Postives = 289/566 (51.06%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
M +VIFL +++IL+ L +I + W P + + + QG+ GP R GN+ ++
Sbjct: 1 MGLVIFLALIVLILIIGL-RIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSEI 60
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
+ +S + +DI R+LPHY W QY E L +
Sbjct: 61 KKMKRESHLSILDPSSNDIFPRILPHYQKWMSQY------------------GETFLYWN 120
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSH-RCTCLYGGQA--- 180
G + R C+ + AKT ++ K G V S + L G +
Sbjct: 121 GTEPRI------CISDPELAKT---------MLSNKLGFFVKSKARPEAVKLVGSKGLVF 180
Query: 181 -QGTSFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEF- 240
+G + H L S L MV+CT KM+E + E TE
Sbjct: 181 IEGADWVRHRRILNPAFSIDRLKI--------MTTVMVDCTLKMLEEWRKESTKEETEHP 240
Query: 241 ----EIADYMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFP 300
E+ + +RLTADII+ + F +S +G ++F L+R + + PG+++ P
Sbjct: 241 KIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELKRCYTTSLNQVSIPGTQYLP 300
Query: 301 SKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLN 360
+ N + L+ +++ + II SR + + YG DLLG+LL + ++ ++
Sbjct: 301 TPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDDLLGILLKAYNTEGKER-KMS 360
Query: 361 LQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLS 420
++ I+ EC+TFFF GHETT+ LL WT MLL+ + +WQ K+R+E+ + C TP E S
Sbjct: 361 IEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECGKEKTPDSETFS 420
Query: 421 KLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKD 480
KL L+NMVI ESLRLY P + L R A +IKLGDLEIPKG ++ IP+L +H + LWG D
Sbjct: 421 KLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPKGTTVVIPLLKMHSDKTLWGSD 480
Query: 481 ANEFKPERFATKPFSGGR----FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSF-TIS 540
A++F P RFA + F+ GPR CIGQ F M+EAK +L M+L +F F ++
Sbjct: 481 ADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFVMIEAKTVLTMILQRFRFISLC 517
Query: 541 DSYRHAPVIVLTIQPKYGVQVCLTPL 552
D Y+H PV +TIQP+YG+ V L PL
Sbjct: 541 DEYKHTPVDNVTIQPQYGLPVMLQPL 517
BLAST of Spg038507 vs. TAIR 10
Match:
AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )
HSP 1 Score: 280.0 bits (715), Expect = 4.0e-75
Identity = 194/562 (34.52%), Postives = 277/562 (49.29%), Query Frame = 0
Query: 1 MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
M ++ + L + LV + Y P + + KQG+ GP+ R L GN+ ++
Sbjct: 56 MELLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREI 115
Query: 61 AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
+ ++ + +DIV R+LPH W QY E L
Sbjct: 116 RKMKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQY------------------GETFLYWQ 175
Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQ---AQ 180
G D R C+ + AK Q L + + K S T L G
Sbjct: 176 GTDPRL------CISDHELAK---QILSNKFVFFSK-----SKTKPEILKLSGNGLIFVN 235
Query: 181 GTSFPPHISYLF-VFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTE--F 240
G + H L FS L MV+CT +M ++ + + TE
Sbjct: 236 GLDWVRHRRILNPAFSMDKL---------KLMTQLMVDCTFRMFLEWKKQRNGVETEQFV 295
Query: 241 EIADYMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYN 300
I+ KRLTADII+ F +S +G ++F LQ+ CA A L FPG ++ P+ N
Sbjct: 296 LISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSN 355
Query: 301 REIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLNLQLI 360
+I L M+V + II +R S YG+DLLG++L E +++ I
Sbjct: 356 LQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIMLTAAS-SNESEKKMSIDEI 415
Query: 361 MDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTL 420
++ECKTFFFAGHETTA LLTW+ MLL+ + +WQ K+R+E+ C P E SKL L
Sbjct: 416 IEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKL 475
Query: 421 LNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEF 480
+N V ESLRLY P L R+A ED+KLG+LEIPKG +I +P+ +H + +WG DA++F
Sbjct: 476 MNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDADKF 535
Query: 481 KPERFATKPFSGGR----FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRH 540
P RFA + F+ GPR CIGQ FA+MEAK +LAM+L +F +S Y+H
Sbjct: 536 NPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADYKH 571
Query: 541 APVIVLTIQPKYGVQVCLTPLN 553
AP LT+QP+Y + V L P++
Sbjct: 596 APADHLTLQPQYDLPVILEPID 571
BLAST of Spg038507 vs. TAIR 10
Match:
AT2G26710.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 279.6 bits (714), Expect = 5.3e-75
Identity = 180/563 (31.97%), Postives = 294/563 (52.22%), Query Frame = 0
Query: 6 FLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDVAALVA 65
F+ +LV+ V L+ + +S W PR+I + +KQG+RGP IGN+ ++ ++
Sbjct: 9 FIPKVLVLSV-ILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMML 68
Query: 66 KSTAGDMSSIDHDIVDRLLPHYTAWTKQYA------FGSDRYRKEVHLLERDGAEAVLDG 125
K+++ M H+I+ R+L Y W K Y FG +R V A+ L
Sbjct: 69 KASSHPM-PFSHNILPRVLSFYHHWRKIYGATFLVWFG-PTFRLTV-------ADPDLIR 128
Query: 126 DGIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGT 185
+ K +K + +V G+ L + KW HR
Sbjct: 129 EIFSKSEFYEKNE-AHPLVKQLEGDGLL---SLKGEKWAH------HR------------ 188
Query: 186 SFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESEL-DSGRTEFEIAD 245
IS F + LL +++ M++ + +L ++G E ++ +
Sbjct: 189 ---KIISPTFHMENLKLL-----------VPVVLKSVTDMVDKWSDKLSENGEVEVDVYE 248
Query: 246 YMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIK 305
+ + LT D+ISRT F +S + G+ +F L LCA+A + + PG RFFP++ N +
Sbjct: 249 WFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYRFFPTRGNLKSW 308
Query: 306 SLKMEVERLLMEIIQSRKDGVEIGR-STC---YGSDLLGMLLNEMQRKREDGFSLNLQLI 365
L E+ + L+++I+ R+ G C DLLG+++ Q K ++ +Q I
Sbjct: 309 KLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI---QAK-----NVTVQDI 368
Query: 366 MDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTL 425
++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K RDE+ +VC P+ +H+ KL
Sbjct: 369 VEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKT 428
Query: 426 LNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEF 485
L+M++NESLRLYPP R A D+KLG +IP G + IP++A+HH + +WG D NEF
Sbjct: 429 LSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEF 488
Query: 486 KPERFATKPFSGGR----FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRH 545
P RFA + FIPF G R CIGQ A+++AK+ LA+++ +F+F ++ +Y+H
Sbjct: 489 NPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQH 517
Query: 546 APVIVLTIQPKYGVQVCLTPLNS 554
AP +++ + P++G + L +
Sbjct: 549 APTVLMLLYPQHGAPITFRRLTN 517
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9FF18 | 1.1e-170 | 57.40 | Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1 | [more] |
Q9ZW95 | 1.7e-166 | 56.86 | Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1 | [more] |
Q9ASR3 | 1.6e-76 | 34.10 | Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1 | [more] |
Q6Z6D6 | 4.4e-74 | 32.33 | Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 ... | [more] |
F4IK45 | 5.7e-74 | 34.52 | Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3CGC0 | 1.9e-221 | 73.20 | cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1 | [more] |
A0A0A0L6Y6 | 3.0e-219 | 72.66 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1 | [more] |
A0A6J1J2Y1 | 3.5e-207 | 70.04 | cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1 | [more] |
A0A6J1F8S1 | 2.5e-205 | 68.90 | cytokinin hydroxylase OS=Cucurbita moschata OX=3662 GN=LOC111441866 PE=3 SV=1 | [more] |
A0A5A7V8T6 | 8.8e-203 | 72.15 | Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... | [more] |
Match Name | E-value | Identity | Description | |
AT5G38450.1 | 7.9e-172 | 57.40 | cytochrome P450, family 735, subfamily A, polypeptide 1 | [more] |
AT1G67110.1 | 1.2e-167 | 56.86 | cytochrome P450, family 735, subfamily A, polypeptide 2 | [more] |
AT2G46960.2 | 1.1e-77 | 34.10 | cytochrome P450, family 709, subfamily B, polypeptide 1 | [more] |
AT2G46950.1 | 4.0e-75 | 34.52 | cytochrome P450, family 709, subfamily B, polypeptide 2 | [more] |
AT2G26710.1 | 5.3e-75 | 31.97 | Cytochrome P450 superfamily protein | [more] |