Spg038507 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg038507
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCytochrome P450, putative
Locationscaffold12: 1930288 .. 1935339 (-)
RNA-Seq ExpressionSpg038507
SyntenySpg038507
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATTGTGATTTTTCTAACAACGCTTTTAGTGATTCTTGTTTCATCACTTGCAAAGATTGCATATGATACTCTCTCATGCTACTGGCTGACCCCTAGGAGGATCAGGAAGACGATGGCAAAGCAAGGCGTTCGCGGCCCTGAGCCCCGGCCGCTGATCGGAAACATCCTCGATGTAGCGGCGCTCGTGGCGAAATCCACCGCAGGCGACATGAGCTCGATCGATCACGACATCGTCGATCGCCTGCTGCCCCATTACACTGCGTGGACAAAGCAATATGGTATGACTATTATTGTTTTTTTGTTTTCATCATCCGTTATGGTGTTAAGAATACGAATAAACGATTAAATATACCATGACCATCCACTTAAATTGTCAACCTCATTGACATATAACTTAATCGGGAGATTAGAAATTCGAATTTTTTCATCTCCCACTATTAGATAAACATGAGGTTCCATCTTTTTGGGTTTCATCGATTCTAATACCATATGAGGTAAATATAAGGTTTTATCTCGAAACTGATTGAGAAGTACAATACATGACTACATAACAGTGTGCAGTGATCTCCCTCACACACTCCAGAAAATCTTGACAACTAAGAGAGAGAAAAGTAGAAAACGAGAAATTTTTTTCATGTCTCCTAATTGTCTACGTTTTAATGGTGGTGTGTAGAGAGGTACTTACACAGGTATGTAGCCAAGTATTGTCCAATTGATAATGAGAGAAGTAACCCATGTACTTTATAAATTGTGAGGTCTCTATTTCATCATTTTGATGTGAAATCCTGATCAACACCCACATGTTGAGAATTTAAAAAAAAAAAAAACCACCTACATAAGTTCATAGTATTAGAATACGAAGTCTTATACATTTGAACTCGTGTAACATCCCCGACGTCATTTTCTCTCAAGTATTATATTATATTATACATAGTCACGTTTGTCAAACTAATTTTAAGGTCTTCAAAGGACACTTGTGTGGGACATGAATTAGGAGTTCAGTTTTCAAATATTGTTACCCACAATTTAGACTCCAAACAAAATACATGATCCGAATACGCAGTAACCTATCAACTTAAGCTTTTGGATCCGATCGATACAGGAAAGAGGTTCATTTACTGGAACGGGACGGAGCCGAGGCTGTGCTTGACGGAGACGGAATTGATAAAAGAACTATTGGCAAAAAACAGCAGTGTGTGTGGGAAATCGTGGCTGCAAAGACAGGGAACCAAACACTTCATAGGGAAAGGATTGTTGATGGCAAATGGGGAAGATTGGTATCATCAACGTCACATCGTTGCACCTGCCTTTATGGGGGACAAGCTCAAGGTACATCTTTCCCTCCTCACATCTCCTATCTCTTCGTCTTTTCATCATCATCATTATTGTCGGCCGATTACGACTTCTTTGATAATGTTTCTGTTTCTTATTCTCTTCGAGAAAGAAAGTCATAAATGGAAAGAGGTTTGATAATTATTATGAATAATACTTGATTGTATACGAGTAACTAGAGACCAAAATCTTGACAACTAAGAAAAAGAATGATAGAAAATGAGAAAACTTTTTTCTCTCTCCTAGATGTCAACGTTTTAATGGTGGTATGCAGTCAAATGTTTTCCAATTATTATTTCTTTTTCTTTTTACATGTATTTGATGAATACTAAATTTTTAAATAACTTTTTAATGGATTTTATAAGCTATAAATGGCAAAATAATAATTTTTTAGGTTAAATTATAAAAAATACCCCTTAACTATGAGATTGGTTTCAATTATACCCATAAACTTTCAAAAGTTTCATTTTAGTCCTTGAAGTTTGAAGTTTGTTTCAGAACACCCTCGAAGACTATTTTTCATTACATTCATGGACGGAAAATGACGTGTTTGCATACATATTGTTGCTCATGTGAATAGATTTGAACACATACGTGAAAAATAAAAAAAAAAACAATAAAAACGCATGTAAACACATCATATTTTGTCAATAAATTTAACGGAAAATGTCTTCAAGGGTAATTTTGAAACAAACTTCAAACTTTAAGGACTAAAAATGAAACTTTTGAAAGTTCAGAAGTATAATCTAAACCAACCTCATAGTTAAAAGGAGTATTTTTGATAATTTAACCTCTTATTTGAGACAATCTAAACAAGGACATATCATGTCTATAAATCAAATTATATATAAAAAAAGAAAAAAGAAAAATTAAGGGTAAAATCTAGAAATTGTTAGAAGAACATACCGTACACAGAGAGATTGACAAAGTTCAAATCAAAATTGTTCCGTTGTACTTTTGTCTGTTAAAAAGAGAAAATATAGGACCCATATGTAAAAACAAATTAATAAATCAGTCGGTGAAGGAAAAAAGAAAAAAAAAAAAAAAAAAAAGAAAAGTAAATTGTTTTAATTGATTTATTTATTATTAGTTTTGATATTTGTAATTATATAATTGGAATTTTTATATGGATTTTGTAATGACGTGAATAGAGCTATGCGGGGTACATGGTGGAATGCACTAAGAAAATGATAGAATCATTCGAGAGCGAGTTGGACTCGGGCCGGACCGAGTTCGAAATCGCCGATTACATGAAGCGACTCACCGCCGACATCATTTCCCGGACCGAGTTCGACACCAGCTCCGACAAAGGCAAACAAATCTTCCATTTGCTCACTCTTCTTCAGCGCCTCTGCGCCCAAGCCAGCCGCCACCTCTGCTTCCCCGGTAGCCGGTAAGACCAAAGACGCGACTGCCTCTACCTATAAGTTTCTTTGTTTTGTTGGTTAGTTACCTTCATTCTATAAATGTTTTCAGAATTGAGTCAAATTTTGAAAAACTAAAATAAAATATGTTTTTAGAAACATGTTTTTGTTTTTTAAATGAACAAAACTTGGCTGCATAACGGTATGTAGGGATCTCCCTACCTATCCAATTAAAATCTTGACAACTAGGAAAGAGGAATGGAGAAAATGAGAATTTTTTTTTTATCTGTCCTCGTTGTCAAGATTTTAATGGTGGTATGCAGGGAGATTCCTACATACCAGTATGCAGGAAGATTCCTACATACCAGTATGCAGCCAAGTTTTTTCCTTTTTAAATTGGCGTTAAGAATTCAATTAGTATAAAGAAATGGTGAGAAAACAAATGGTCTGAGAGTGTTTAGTTTTTTTCTCTATTACTTTATTCCTTCTTAAATTTTTCATAATTGAAAGATTATATACATGAAAAGTTAAACCCGTTTGATGACCATTTGGTTTTTGAAAAATATATAATTGTTTTCTCCTAATTTTCTTAGAATGATTTTCATTTTTGTTAAGGACATATTTGAATTCTTAACCATTTTTTTTTTTTTTTTTTAAGTTTTCGATAAGTACTTGTTTTAGTTTTCAAAACTTGATTTGGTTTTTGAAAATATGAGAAGAAGGTAAACATAAAAAAATAACTTAGGAATGGAAGTGGTGTTTGTAAGCTTAATTTTCAAAAACCAAAAACCAAATACACCAAAATTTGACCCAACTTTAAAACTTTTACCTTTCTAATAATCTTTTTTGAGCGATCGTCTCATTCGTCTATACCTTAAACTTGCAAGGATGTATATGGTCGTTCGAGTTTATTAAGGTGATTGGAGTTGTTGTTTCAGGTTTTTTCCAAGTAAGTACAACAGAGAAATAAAGTCACTAAAGATGGAGGTGGAGAGGTTGTTAATGGAGATCATTCAAAGCAGAAAAGATGGTGTGGAGATTGGAAGAAGCACTTGCTATGGAAGTGATTTGTTGGGAATGCTGTTGAACGAGATGCAAAGGAAGAGAGAAGATGGATTCAGCTTGAACTTGCAGCTGATAATGGATGAATGCAAGACTTTCTTCTTTGCTGGCCATGAAACCACTGCTCTCTTGCTCACTTGGACCATCATGTTGCTCGCCACTAACCCTAATTGGCAACACAAGGTTCGTGATGAACTCAACCAAGTTTGCAATGGTGGAACCACTCCCTCTATTGAGCATCTCTCCAAGCTCACCTTGGTAAGCCCACTATAAAATTTCTCAAACGTTTCAGTCCCGTTTGATATCCGTTTGGTTTTTGAAAATTATGCTTGTTTTCTCCGAATTTCCTTACGATGATTTTCGTCTTTGTTAAGGACACGTCTGAATTCCTAGCCAAATTTAAAAAACAAATACAAGTTTTTTTGAAAAACACTTTCTTTTAAAGAAAAAAATTCAAAACTTTTGATTTGGTTTTTTAAAGCATGGAACGAATGTAGATAACAAAACAAATAAACTTATAAGTAGAAGTAGTGTTTATAAGCTTAATTTTTAAAACCAAATGATTATGATCAAACATGACCTAAATATTTCTAATTCAATCTGCCTAATTCTCTAATAATTTCTCTCGAGCTTCGTGGATCAAATTAATAATATATCCGAATCCCACTAAATAAAGGTATGTAATTATATATATAAAAGGTTAAATTTGGGTTTAGTGGTGATCTAACATATATTTGGATATTCTACACTCAAAGGTCACGATTAGTTTGAATATTAATATAGTTCTCCGCTTGAGAATAATCAAATTAATATCTTAACTGACCCTGATACACGATATCATTTTTTTGATATGATATGTATTTTTGCAGTTAAACATGGTGATAAATGAATCCCTGAGGCTTTACCCACCGGCAACGGTGCTTCCTAGAATGGCGTTCGAGGACATAAAGCTAGGAGACTTGGAGATCCCGAAAGGGCTATCGATATGGATCCCGGTGCTCGCGATTCATCATAGCGAAGAGTTATGGGGAAAAGATGCAAACGAGTTCAAGCCAGAACGATTTGCCACAAAACCATTCAGTGGTGGACGTTTCATTCCATTTGCAGCTGGACCAAGAAACTGCATTGGGCAAGCTTTTGCAATGATGGAAGCTAAGATCATCTTGGCTATGTTACTCTCAAAGTTTAGCTTCACTATTTCAGATTCTTATCGCCATGCTCCTGTTATTGTCCTCACCATACAGCCTAAGTATGGAGTTCAAGTTTGTTTGACCCCATTGAATTCTTGA

mRNA sequence

ATGGCGATTGTGATTTTTCTAACAACGCTTTTAGTGATTCTTGTTTCATCACTTGCAAAGATTGCATATGATACTCTCTCATGCTACTGGCTGACCCCTAGGAGGATCAGGAAGACGATGGCAAAGCAAGGCGTTCGCGGCCCTGAGCCCCGGCCGCTGATCGGAAACATCCTCGATGTAGCGGCGCTCGTGGCGAAATCCACCGCAGGCGACATGAGCTCGATCGATCACGACATCGTCGATCGCCTGCTGCCCCATTACACTGCGTGGACAAAGCAATATGCTTTTGGATCCGATCGATACAGGAAAGAGGTTCATTTACTGGAACGGGACGGAGCCGAGGCTGTGCTTGACGGAGACGGAATTGATAAAAGAACTATTGGCAAAAAACAGCAGTGTGTGTGGGAAATCGTGGCTGCAAAGACAGGGAACCAAACACTTCATAGGGAAAGGATTGTTGATGGCAAATGGGGAAGATTGGTATCATCAACGTCACATCGTTGCACCTGCCTTTATGGGGGACAAGCTCAAGGTACATCTTTCCCTCCTCACATCTCCTATCTCTTCGTCTTTTCATCATCATCATTATTGTCGGCCGATTACGACTTCTTTGATAATAGCTATGCGGGGTACATGGTGGAATGCACTAAGAAAATGATAGAATCATTCGAGAGCGAGTTGGACTCGGGCCGGACCGAGTTCGAAATCGCCGATTACATGAAGCGACTCACCGCCGACATCATTTCCCGGACCGAGTTCGACACCAGCTCCGACAAAGGCAAACAAATCTTCCATTTGCTCACTCTTCTTCAGCGCCTCTGCGCCCAAGCCAGCCGCCACCTCTGCTTCCCCGGTAGCCGGTTTTTTCCAAGTAAGTACAACAGAGAAATAAAGTCACTAAAGATGGAGGTGGAGAGGTTGTTAATGGAGATCATTCAAAGCAGAAAAGATGGTGTGGAGATTGGAAGAAGCACTTGCTATGGAAGTGATTTGTTGGGAATGCTGTTGAACGAGATGCAAAGGAAGAGAGAAGATGGATTCAGCTTGAACTTGCAGCTGATAATGGATGAATGCAAGACTTTCTTCTTTGCTGGCCATGAAACCACTGCTCTCTTGCTCACTTGGACCATCATGTTGCTCGCCACTAACCCTAATTGGCAACACAAGGTTCGTGATGAACTCAACCAAGTTTGCAATGGTGGAACCACTCCCTCTATTGAGCATCTCTCCAAGCTCACCTTGTTAAACATGGTGATAAATGAATCCCTGAGGCTTTACCCACCGGCAACGGTGCTTCCTAGAATGGCGTTCGAGGACATAAAGCTAGGAGACTTGGAGATCCCGAAAGGGCTATCGATATGGATCCCGGTGCTCGCGATTCATCATAGCGAAGAGTTATGGGGAAAAGATGCAAACGAGTTCAAGCCAGAACGATTTGCCACAAAACCATTCAGTGGTGGACGTTTCATTCCATTTGCAGCTGGACCAAGAAACTGCATTGGGCAAGCTTTTGCAATGATGGAAGCTAAGATCATCTTGGCTATGTTACTCTCAAAGTTTAGCTTCACTATTTCAGATTCTTATCGCCATGCTCCTGTTATTGTCCTCACCATACAGCCTAAGTATGGAGTTCAAGTTTGTTTGACCCCATTGAATTCTTGA

Coding sequence (CDS)

ATGGCGATTGTGATTTTTCTAACAACGCTTTTAGTGATTCTTGTTTCATCACTTGCAAAGATTGCATATGATACTCTCTCATGCTACTGGCTGACCCCTAGGAGGATCAGGAAGACGATGGCAAAGCAAGGCGTTCGCGGCCCTGAGCCCCGGCCGCTGATCGGAAACATCCTCGATGTAGCGGCGCTCGTGGCGAAATCCACCGCAGGCGACATGAGCTCGATCGATCACGACATCGTCGATCGCCTGCTGCCCCATTACACTGCGTGGACAAAGCAATATGCTTTTGGATCCGATCGATACAGGAAAGAGGTTCATTTACTGGAACGGGACGGAGCCGAGGCTGTGCTTGACGGAGACGGAATTGATAAAAGAACTATTGGCAAAAAACAGCAGTGTGTGTGGGAAATCGTGGCTGCAAAGACAGGGAACCAAACACTTCATAGGGAAAGGATTGTTGATGGCAAATGGGGAAGATTGGTATCATCAACGTCACATCGTTGCACCTGCCTTTATGGGGGACAAGCTCAAGGTACATCTTTCCCTCCTCACATCTCCTATCTCTTCGTCTTTTCATCATCATCATTATTGTCGGCCGATTACGACTTCTTTGATAATAGCTATGCGGGGTACATGGTGGAATGCACTAAGAAAATGATAGAATCATTCGAGAGCGAGTTGGACTCGGGCCGGACCGAGTTCGAAATCGCCGATTACATGAAGCGACTCACCGCCGACATCATTTCCCGGACCGAGTTCGACACCAGCTCCGACAAAGGCAAACAAATCTTCCATTTGCTCACTCTTCTTCAGCGCCTCTGCGCCCAAGCCAGCCGCCACCTCTGCTTCCCCGGTAGCCGGTTTTTTCCAAGTAAGTACAACAGAGAAATAAAGTCACTAAAGATGGAGGTGGAGAGGTTGTTAATGGAGATCATTCAAAGCAGAAAAGATGGTGTGGAGATTGGAAGAAGCACTTGCTATGGAAGTGATTTGTTGGGAATGCTGTTGAACGAGATGCAAAGGAAGAGAGAAGATGGATTCAGCTTGAACTTGCAGCTGATAATGGATGAATGCAAGACTTTCTTCTTTGCTGGCCATGAAACCACTGCTCTCTTGCTCACTTGGACCATCATGTTGCTCGCCACTAACCCTAATTGGCAACACAAGGTTCGTGATGAACTCAACCAAGTTTGCAATGGTGGAACCACTCCCTCTATTGAGCATCTCTCCAAGCTCACCTTGTTAAACATGGTGATAAATGAATCCCTGAGGCTTTACCCACCGGCAACGGTGCTTCCTAGAATGGCGTTCGAGGACATAAAGCTAGGAGACTTGGAGATCCCGAAAGGGCTATCGATATGGATCCCGGTGCTCGCGATTCATCATAGCGAAGAGTTATGGGGAAAAGATGCAAACGAGTTCAAGCCAGAACGATTTGCCACAAAACCATTCAGTGGTGGACGTTTCATTCCATTTGCAGCTGGACCAAGAAACTGCATTGGGCAAGCTTTTGCAATGATGGAAGCTAAGATCATCTTGGCTATGTTACTCTCAAAGTTTAGCTTCACTATTTCAGATTCTTATCGCCATGCTCCTGTTATTGTCCTCACCATACAGCCTAAGTATGGAGTTCAAGTTTGTTTGACCCCATTGAATTCTTGA

Protein sequence

MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDVAALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGDGIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTSFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTIQPKYGVQVCLTPLNS
Homology
BLAST of Spg038507 vs. NCBI nr
Match: XP_038905687.1 (cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 781.2 bits (2016), Expect = 6.0e-222
Identity = 414/555 (74.59%), Postives = 447/555 (80.54%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           MAIVIFLTT+LVI +SSLAKIAYDT+SCYWLTPRRIRK M KQGV GP+PRPL+GNILDV
Sbjct: 1   MAIVIFLTTILVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVCGPDPRPLLGNILDV 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
           AALVAKSTA DM SIDH+IV RLLPHYT WTKQY                   +  +  +
Sbjct: 61  AALVAKSTANDMKSIDHNIVGRLLPHYTTWTKQY------------------GKRFIYWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRC-TCLYGGQAQGT 180
           G + R    + + + E++ +K  N        V GK       T H     L     +  
Sbjct: 121 GTEPRLCLAETELIKEVL-SKNSN--------VCGKSWLQRQGTKHFIGKGLLMANGEDW 180

Query: 181 SFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADY 240
               HI     F    L          SYAG MVECTKKMI+S E+E DSGRTEFEIADY
Sbjct: 181 YHQRHI-VAPAFMGDKL---------KSYAGLMVECTKKMIQSLETEFDSGRTEFEIADY 240

Query: 241 MKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKS 300
           MK+LTADIISRTEFD +SDKGK+IFHLLTLLQRLCAQASRHLC PGSRFFPSKYNREIKS
Sbjct: 241 MKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQASRHLCLPGSRFFPSKYNREIKS 300

Query: 301 LKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED-GFSLNLQLIMDEC 360
           LKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED GFSLNLQLIMDEC
Sbjct: 301 LKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDNGFSLNLQLIMDEC 360

Query: 361 KTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMV 420
           KTFFFAGHETTALLLTWTIMLLATNP+WQHKVR E+NQVCN G +PS+EHLSKLTLLNMV
Sbjct: 361 KTFFFAGHETTALLLTWTIMLLATNPDWQHKVRHEVNQVCNNGGSPSVEHLSKLTLLNMV 420

Query: 421 INESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPER 480
           INESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWIPVLAIHHSEELWG+DANEFKPER
Sbjct: 421 INESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGEDANEFKPER 480

Query: 481 FATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTI 540
           FA+KPFSGGRFIPFA+GPRNCIGQ FA+MEAKIILAM +SKFSFTISDSYRHAPV VLTI
Sbjct: 481 FASKPFSGGRFIPFASGPRNCIGQGFALMEAKIILAMFISKFSFTISDSYRHAPVSVLTI 518

Query: 541 QPKYGVQVCLTPLNS 554
           QPKYGVQV LTP+NS
Sbjct: 541 QPKYGVQVYLTPINS 518

BLAST of Spg038507 vs. NCBI nr
Match: XP_008461587.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 778.5 bits (2009), Expect = 3.9e-221
Identity = 407/556 (73.20%), Postives = 444/556 (79.86%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           MAI+IFLTTLLVI +SSLAKIAYDT+SCYWLTPRRIRK M KQGVRGP PRPL+GNILDV
Sbjct: 1   MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
           AAL+AKSTA DMSSIDH+IVDRLLPHYT WTKQY                   +  +  +
Sbjct: 61  AALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQY------------------GKRFIYWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
           G++ R    + + + E+++  +         +    W +   +       L     +   
Sbjct: 121 GMEPRLCLAETELIKEVLSKNS--------NVCGRSWLQRQGTKHFIGKGLLMANGEDWY 180

Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
              HI     F    L          SYAG MVECT K+I+S E+ELDSGRTEFEIADYM
Sbjct: 181 HQRHI-VAPSFMGDKL---------KSYAGLMVECTDKLIQSLETELDSGRTEFEIADYM 240

Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
           K+LTADIISRTEFD +SDKG QIFHLLTLLQ LCAQASRHLC PGSRFFPSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSL 300

Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED---GFSLNLQLIMDE 360
           KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRK++D   GFSLNLQLIMDE
Sbjct: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKKDDGDNGFSLNLQLIMDE 360

Query: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNM 420
           CKTFFFAGHETTALLLTWTIMLLATNPNWQ KVR E+NQVC+ G TPS+EHLSK TLLNM
Sbjct: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQVCHNGATPSVEHLSKFTLLNM 420

Query: 421 VINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPE 480
           VINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWIPVLAIHHSEELWGKD NEFKPE
Sbjct: 421 VINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPE 480

Query: 481 RFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLT 540
           RFA+KPFSGGRFIPFAAGPRNC+GQ FA+MEAKIILAMLLSKFSFTISDSYRHAPV VLT
Sbjct: 481 RFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIILAMLLSKFSFTISDSYRHAPVSVLT 520

Query: 541 IQPKYGVQVCLTPLNS 554
           IQPKYGVQV LTP+NS
Sbjct: 541 IQPKYGVQVYLTPINS 520

BLAST of Spg038507 vs. NCBI nr
Match: XP_004145660.1 (cytokinin hydroxylase [Cucumis sativus] >KGN57750.1 hypothetical protein Csa_009800 [Cucumis sativus])

HSP 1 Score: 771.2 bits (1990), Expect = 6.2e-219
Identity = 404/556 (72.66%), Postives = 441/556 (79.32%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           MAI+I LTTLLVI +SSLAKIAYDT+SCYWLTPRRIRK M KQGVRGP PRPL+GNILDV
Sbjct: 1   MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
           AAL+ KSTA DMSSIDH+IVDRLLPHYT WTKQY                   +  +  +
Sbjct: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQY------------------GKRFIYWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
           G++ R    + + + E+++  +         +    W +   +       L     +   
Sbjct: 121 GMEPRLCLAETELIKEVLSKNS--------NVCGRSWLQRQGTKHFIGKGLLMANGEDWY 180

Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
              HI     F    L          SYAG MVECT K+I+S E+E DSGRTEFEIADYM
Sbjct: 181 HQRHI-VAPSFMGDKL---------KSYAGLMVECTNKLIQSLETEFDSGRTEFEIADYM 240

Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
           K+LTADIISRTEFD +SDKG QIFHLLTLLQ LCAQASRHLC PGSRFFPSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSL 300

Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED---GFSLNLQLIMDE 360
           KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQ++RED   GFSLNLQLIMDE
Sbjct: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQKRREDGDNGFSLNLQLIMDE 360

Query: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNM 420
           CKTFFFAGHETTALLLTWTIMLLATNPNWQ KVR E+NQVC+ G TPS+EHLSK TLLNM
Sbjct: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQVCHNGATPSVEHLSKFTLLNM 420

Query: 421 VINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPE 480
           VINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWIPVLAIHHSEELWGKD NEFKPE
Sbjct: 421 VINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPE 480

Query: 481 RFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLT 540
           RFA+KPFSGGRFIPFAAGPRNCIGQ FA+MEAKIILAMLLSKFSF+ISDSYRHAPV VLT
Sbjct: 481 RFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIILAMLLSKFSFSISDSYRHAPVSVLT 520

Query: 541 IQPKYGVQVCLTPLNS 554
           IQPKYGVQV LTP+NS
Sbjct: 541 IQPKYGVQVYLTPINS 520

BLAST of Spg038507 vs. NCBI nr
Match: XP_022983651.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 731.1 bits (1886), Expect = 7.1e-207
Identity = 388/554 (70.04%), Postives = 426/554 (76.90%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           MA +IFLTTL VI +SSLAKI+YDT S Y LTPRRIRKTM +QGVRGP+PR LIGNILDV
Sbjct: 1   MAFIIFLTTLFVIFISSLAKISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
             LVAKSTA DMSSIDHDIVDRLLPHYT WTKQY                   +  +  +
Sbjct: 61  VGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQY------------------GKRFIYWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
           G + R    + + + E++  K+         +    W +   +       L     +   
Sbjct: 121 GTEPRLCLAETELIKELLGKKSS--------VCGRSWLQRQGTKHFIGKGLLMANGEDWY 180

Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
              HI     F    L          SYAG+MVECT KMI+S E+ELDSGRTEFEIADYM
Sbjct: 181 HQRHI-VAPAFMGDKL---------KSYAGFMVECTNKMIQSMETELDSGRTEFEIADYM 240

Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
           K+LTADIISRTEFDT SDKG QIFHLLTLLQRLCAQASRHLC PGSRF PSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSKYNREIKSL 300

Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM-QRKREDGFSLNLQLIMDECK 360
           K EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLLNEM QRK     +LNLQLI+DECK
Sbjct: 301 KKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQRKNSTTTTLNLQLIIDECK 360

Query: 361 TFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVI 420
           TFFFAGHETTALLLTWTIMLLATNPNWQH VR E+NQVCN   TPSI+HLSKLTLLNMVI
Sbjct: 361 TFFFAGHETTALLLTWTIMLLATNPNWQHMVRAEVNQVCN-ARTPSIQHLSKLTLLNMVI 420

Query: 421 NESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERF 480
           NESLRLYPPATVLPRMA ED+KLGD++IPKGLSIWIPVLAIHHSEELWGKDAN+F P RF
Sbjct: 421 NESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGKDANQFNPNRF 480

Query: 481 ATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTIQ 540
           A K F GGRFIPFA GPRNCIGQ FA+MEAKIILAM LSKFSFTISD+YRHAPV+VLTIQ
Sbjct: 481 AAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKFSFTISDTYRHAPVVVLTIQ 517

Query: 541 PKYGVQVCLTPLNS 554
           PK+GVQVCLTP +S
Sbjct: 541 PKHGVQVCLTPFSS 517

BLAST of Spg038507 vs. NCBI nr
Match: XP_023528555.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 726.1 bits (1873), Expect = 2.3e-205
Identity = 383/557 (68.76%), Postives = 427/557 (76.66%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           MA +IFLTTLL+I ++SLA+I+YDT S Y LTPRRIRKTM +QGVRGP+PR LIGNILDV
Sbjct: 1   MAFIIFLTTLLLIFIASLARISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
           A LVAKSTA DMSSIDHDIVDRLLPHYT WTKQY                   +  +  +
Sbjct: 61  AGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQY------------------GKRFIYWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
           G + R    + + + E++  K+         +    W +   +       L     +   
Sbjct: 121 GTEPRLCLAETELIKELLGKKSS--------VCGRSWLQRQGTKHFIGKGLLMANGEDWY 180

Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
              HI     F    L          SYAG+MVECT KMI+S E+EL+SGRTEFEIADYM
Sbjct: 181 HQRHI-VAPAFMGDKL---------KSYAGFMVECTNKMIQSMETELESGRTEFEIADYM 240

Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
           K+LTADIISRTEFDT SDKG QIFHLLTLLQRLCAQASRHLC PGSRF PSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSKYNREIKSL 300

Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED----GFSLNLQLIMD 360
           K EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLLNE+Q+++        +LNLQLIMD
Sbjct: 301 KKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNELQQRKNSSTTTSTTLNLQLIMD 360

Query: 361 ECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLN 420
           ECKTFFFAGHETTALLLTWTIMLLATNPNWQH VR ++NQVCN   TPSI+HLSKLTLLN
Sbjct: 361 ECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN-ARTPSIQHLSKLTLLN 420

Query: 421 MVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKP 480
           MVINESLRLYPPATVLPRMA ED+KLGD++IPKGLSIWIPVLAIHHSEELWGKDAN+F P
Sbjct: 421 MVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGKDANQFNP 480

Query: 481 ERFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVL 540
            RF  K F GGRFIPFA GPRNCIGQ FA+MEAKIILAMLLSKF FTISD+YRHAPV+VL
Sbjct: 481 NRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMLLSKFRFTISDTYRHAPVVVL 520

Query: 541 TIQPKYGVQVCLTPLNS 554
           TIQPKYGVQVCLTP  S
Sbjct: 541 TIQPKYGVQVCLTPFTS 520

BLAST of Spg038507 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 1.1e-170
Identity = 318/554 (57.40%), Postives = 393/554 (70.94%), Query Frame = 0

Query: 3   IVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDVAA 62
           ++  L +LLVI V+++ ++ YDT+SCYWLTPRRI+K M +QGV GP+PRPL GNIL+++A
Sbjct: 2   LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISA 61

Query: 63  LVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGDGI 122
           +V++S + D  SI HDIV RLLPHY AW+KQY                   +  +  +G 
Sbjct: 62  MVSQSASKDCDSIHHDIVGRLLPHYVAWSKQY------------------GKRFIVWNGT 121

Query: 123 DKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGK-WGRLVSSTSHRCTCLYGGQAQGTSF 182
           D R    + + + E++    G         V G+ W +   + +     L     Q    
Sbjct: 122 DPRLCLTETELIKELLMKHNG---------VSGRSWLQQQGTKNFIGRGLLMANGQDWHH 181

Query: 183 PPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYMK 242
             H++    F+   L           YA +MVECT K++E    E+  G  E EI + M 
Sbjct: 182 QRHLA-APAFTGERL---------KGYARHMVECTSKLVERLRKEVGEGANEVEIGEEMH 241

Query: 243 RLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSLK 302
           +LTADIISRT+F +S +KGK++F+ LT+LQR CAQA+RHLCFPGSRF PSKYNREIKSLK
Sbjct: 242 KLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLCFPGSRFLPSKYNREIKSLK 301

Query: 303 MEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQ-RKREDGFSLNLQLIMDECKT 362
            EVERLL+EIIQSR+D  E+GRS+ +G DLLG+LLNEM   K  +  + NLQLIMDECKT
Sbjct: 302 KEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKT 361

Query: 363 FFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVIN 422
           FFFAGHETTALLLTWT MLLA NP WQ KVR+E+ +V      PS++ LSKLT L+ VIN
Sbjct: 362 FFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVIN 421

Query: 423 ESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERFA 482
           ESLRLYPPAT+LPRMAFED+KLGDL IPKGLSIWIPVLAIHHSEELWGKDAN+F PERF 
Sbjct: 422 ESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERFG 481

Query: 483 TKPFSGGR-FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTIQ 542
            +PF+ GR FIPFAAGPRNCIGQ FA+MEAKIILA L+SKF+FTIS +YRHAP++VLTI+
Sbjct: 482 GRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIK 518

Query: 543 PKYGVQVCLTPLNS 554
           PKYGVQV L PL S
Sbjct: 542 PKYGVQVILKPLVS 518

BLAST of Spg038507 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.7e-166
Identity = 315/554 (56.86%), Postives = 387/554 (69.86%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           M + + L  +LVI+++ + ++ YD++ CY+LTPRRI+K M +QG+ GP+PR L GNI+D+
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
           + +++ S + D SSI H+IV RLLPHY +W+KQY                   +  +  +
Sbjct: 61  SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQY------------------GKRFIMWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGK-WGRLVSSTSHRCTCLYGGQAQGT 180
           G + R       C+ E    K   + L +   V GK W +   +       L     +  
Sbjct: 121 GTEPRL------CLTETEMIK---ELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAW 180

Query: 181 SFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADY 240
               H++    F+   L           YA +MVECTK M E    E+     E EI + 
Sbjct: 181 HHQRHMA-APAFTRDRL---------KGYAKHMVECTKMMAERLRKEVGE---EVEIGEE 240

Query: 241 MKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKS 300
           M+RLTADIISRTEF +S DKGK++F LLT+LQRLCAQA+RHLCFPGSRF PSKYNREIKS
Sbjct: 241 MRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPGSRFLPSKYNREIKS 300

Query: 301 LKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLNLQLIMDECK 360
           LK EVERLLMEII SRKD VEIGRS+ YG DLLG+LLN+M   +    +LN+Q+IMDECK
Sbjct: 301 LKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNKN---NLNVQMIMDECK 360

Query: 361 TFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVI 420
           TFFF GHETT+LLLTWT+MLLA NP WQ  VRDE+ QVC     PS+E LS LT LN VI
Sbjct: 361 TFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVI 420

Query: 421 NESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERF 480
           NESLRLYPPAT+LPRMAFEDIKLGDL IPKGLSIWIPVLAIHHS ELWG+DANEF PERF
Sbjct: 421 NESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERF 480

Query: 481 ATKPFSGGR-FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTI 540
            T+ F+  R F+PFAAGPRNCIGQ FAMMEAKIILAML+SKFSF IS++YRHAP++VLTI
Sbjct: 481 TTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTI 511

Query: 541 QPKYGVQVCLTPLN 553
           +PKYGVQ+ L PL+
Sbjct: 541 KPKYGVQLVLKPLD 511

BLAST of Spg038507 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.6e-76
Identity = 193/566 (34.10%), Postives = 289/566 (51.06%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           M +VIFL  +++IL+  L +I    +   W  P  + + +  QG+ GP  R   GN+ ++
Sbjct: 1   MGLVIFLALIVLILIIGL-RIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSEI 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
             +  +S    +    +DI  R+LPHY  W  QY                   E  L  +
Sbjct: 61  KKMKRESHLSILDPSSNDIFPRILPHYQKWMSQY------------------GETFLYWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSH-RCTCLYGGQA--- 180
           G + R       C+ +   AKT         ++  K G  V S +      L G +    
Sbjct: 121 GTEPRI------CISDPELAKT---------MLSNKLGFFVKSKARPEAVKLVGSKGLVF 180

Query: 181 -QGTSFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEF- 240
            +G  +  H   L    S   L              MV+CT KM+E +  E     TE  
Sbjct: 181 IEGADWVRHRRILNPAFSIDRLKI--------MTTVMVDCTLKMLEEWRKESTKEETEHP 240

Query: 241 ----EIADYMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFP 300
               E+ +  +RLTADII+ + F +S  +G ++F     L+R    +   +  PG+++ P
Sbjct: 241 KIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELKRCYTTSLNQVSIPGTQYLP 300

Query: 301 SKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLN 360
           +  N  +  L+ +++  +  II SR     +   + YG DLLG+LL     + ++   ++
Sbjct: 301 TPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDDLLGILLKAYNTEGKER-KMS 360

Query: 361 LQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLS 420
           ++ I+ EC+TFFF GHETT+ LL WT MLL+ + +WQ K+R+E+ + C    TP  E  S
Sbjct: 361 IEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECGKEKTPDSETFS 420

Query: 421 KLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKD 480
           KL L+NMVI ESLRLY P + L R A  +IKLGDLEIPKG ++ IP+L +H  + LWG D
Sbjct: 421 KLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPKGTTVVIPLLKMHSDKTLWGSD 480

Query: 481 ANEFKPERFATKPFSGGR----FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSF-TIS 540
           A++F P RFA             + F+ GPR CIGQ F M+EAK +L M+L +F F ++ 
Sbjct: 481 ADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFVMIEAKTVLTMILQRFRFISLC 517

Query: 541 DSYRHAPVIVLTIQPKYGVQVCLTPL 552
           D Y+H PV  +TIQP+YG+ V L PL
Sbjct: 541 DEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Spg038507 vs. ExPASy Swiss-Prot
Match: Q6Z6D6 (Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 4.4e-74
Identity = 183/566 (32.33%), Postives = 272/566 (48.06%), Query Frame = 0

Query: 30  WLTPRRIRKTMAKQGVRGPEPRPLIGNILDVAALVAKSTAGDMS-SIDHDIVDRLLPHYT 89
           W  PRR+    A QGVRGP  R L+G++ ++ AL+A++++  MS    H+ + R+L  Y 
Sbjct: 42  WWRPRRLEAHFAAQGVRGPPYRFLLGSVREMVALMAEASSKPMSPPTSHNALPRVLAFYH 101

Query: 90  AWTKQYA------FG--------SDRYRKEVHLLERDGAEAV--------LDGDGIDKRT 149
            W K Y       FG             +E+ L   D  +          L+GDG     
Sbjct: 102 YWRKIYGHRFLIWFGPTPRLTVAEPELIREIFLTRADAFDRYEAHPVVRQLEGDG----- 161

Query: 150 IGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTSFPPHIS 209
                     +V+       LHR  + D                          +P +++
Sbjct: 162 ----------LVSLHGDKWALHRRVLTD------------------------AFYPDNLN 221

Query: 210 YLF--VFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYMKRLT 269
            L   V  S + L+A +        G M E              SG  E ++A++ + +T
Sbjct: 222 RLIPHVGKSVAALAAKW--------GAMAEAG-----------GSGEVEVDVAEWFQAVT 281

Query: 270 ADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSLKMEV 329
            + I+R  F  S D G+ +F +   L    ++A R +  PG RF P+K NR    L  E+
Sbjct: 282 EEAITRATFGRSYDDGRVVFAMQGQLMAFASEAFRKVLVPGYRFLPTKKNRLSWRLDREI 341

Query: 330 ERLLMEIIQSRKDGVEIGRSTCYGS--DLLGMLLNEMQ----------RKREDGFSLNLQ 389
            R LM +I  R D  E G     GS  DLLG+++N              K     ++ ++
Sbjct: 342 RRSLMRLIGRRSDEAEQGEKADDGSFRDLLGLMINAGAAAATRGNAGGEKNSPAAAIPVE 401

Query: 390 LIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKL 449
            +++ECKTFFFAG +TT  LLTW  +LLA +P+WQ + R E+  VC  G  PS EHL KL
Sbjct: 402 DMLEECKTFFFAGKQTTTNLLTWATVLLAMHPDWQERARREVFDVCGAGELPSKEHLPKL 461

Query: 450 TLLNMVINESLRLYPPATVLPRMAFEDIKLGD-LEIPKGLSIWIPVLAIHHSEELWGKDA 509
             L M++NE+LRLYPPA    R A  D++L D   IP+ + + +P++AIHH    WG DA
Sbjct: 462 KTLGMIMNETLRLYPPAVATIRRAKVDVQLSDGCMIPRDMELLVPIMAIHHDTRYWGPDA 521

Query: 510 NEFKPERFATKPFSGGR----FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDS 554
           ++F P RFA       +    FIPF  G R C+GQ  A +EAK+ +A+LL +F    S +
Sbjct: 522 SQFNPARFANGASKAAKHPLAFIPFGLGSRMCVGQNLARLEAKLTMAILLQRFEIRTSPN 549

BLAST of Spg038507 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 5.7e-74
Identity = 194/562 (34.52%), Postives = 277/562 (49.29%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           M ++  +  L + LV  +    Y         P  + +   KQG+ GP+ R L GN+ ++
Sbjct: 56  MELLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREI 115

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
             +  ++    +    +DIV R+LPH   W  QY                   E  L   
Sbjct: 116 RKMKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQY------------------GETFLYWQ 175

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQ---AQ 180
           G D R       C+ +   AK   Q L  + +   K     S T      L G       
Sbjct: 176 GTDPRL------CISDHELAK---QILSNKFVFFSK-----SKTKPEILKLSGNGLIFVN 235

Query: 181 GTSFPPHISYLF-VFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTE--F 240
           G  +  H   L   FS   L               MV+CT +M   ++ + +   TE   
Sbjct: 236 GLDWVRHRRILNPAFSMDKL---------KLMTQLMVDCTFRMFLEWKKQRNGVETEQFV 295

Query: 241 EIADYMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYN 300
            I+   KRLTADII+   F +S  +G ++F     LQ+ CA A   L FPG ++ P+  N
Sbjct: 296 LISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSN 355

Query: 301 REIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLNLQLI 360
            +I  L M+V   +  II +R        S  YG+DLLG++L       E    +++  I
Sbjct: 356 LQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIMLTAAS-SNESEKKMSIDEI 415

Query: 361 MDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTL 420
           ++ECKTFFFAGHETTA LLTW+ MLL+ + +WQ K+R+E+   C     P  E  SKL L
Sbjct: 416 IEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKL 475

Query: 421 LNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEF 480
           +N V  ESLRLY P   L R+A ED+KLG+LEIPKG +I +P+  +H  + +WG DA++F
Sbjct: 476 MNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDADKF 535

Query: 481 KPERFATKPFSGGR----FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRH 540
            P RFA             + F+ GPR CIGQ FA+MEAK +LAM+L +F   +S  Y+H
Sbjct: 536 NPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADYKH 571

Query: 541 APVIVLTIQPKYGVQVCLTPLN 553
           AP   LT+QP+Y + V L P++
Sbjct: 596 APADHLTLQPQYDLPVILEPID 571

BLAST of Spg038507 vs. ExPASy TrEMBL
Match: A0A1S3CGC0 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.9e-221
Identity = 407/556 (73.20%), Postives = 444/556 (79.86%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           MAI+IFLTTLLVI +SSLAKIAYDT+SCYWLTPRRIRK M KQGVRGP PRPL+GNILDV
Sbjct: 1   MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
           AAL+AKSTA DMSSIDH+IVDRLLPHYT WTKQY                   +  +  +
Sbjct: 61  AALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQY------------------GKRFIYWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
           G++ R    + + + E+++  +         +    W +   +       L     +   
Sbjct: 121 GMEPRLCLAETELIKEVLSKNS--------NVCGRSWLQRQGTKHFIGKGLLMANGEDWY 180

Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
              HI     F    L          SYAG MVECT K+I+S E+ELDSGRTEFEIADYM
Sbjct: 181 HQRHI-VAPSFMGDKL---------KSYAGLMVECTDKLIQSLETELDSGRTEFEIADYM 240

Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
           K+LTADIISRTEFD +SDKG QIFHLLTLLQ LCAQASRHLC PGSRFFPSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSL 300

Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED---GFSLNLQLIMDE 360
           KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRK++D   GFSLNLQLIMDE
Sbjct: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKKDDGDNGFSLNLQLIMDE 360

Query: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNM 420
           CKTFFFAGHETTALLLTWTIMLLATNPNWQ KVR E+NQVC+ G TPS+EHLSK TLLNM
Sbjct: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQVCHNGATPSVEHLSKFTLLNM 420

Query: 421 VINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPE 480
           VINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWIPVLAIHHSEELWGKD NEFKPE
Sbjct: 421 VINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPE 480

Query: 481 RFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLT 540
           RFA+KPFSGGRFIPFAAGPRNC+GQ FA+MEAKIILAMLLSKFSFTISDSYRHAPV VLT
Sbjct: 481 RFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIILAMLLSKFSFTISDSYRHAPVSVLT 520

Query: 541 IQPKYGVQVCLTPLNS 554
           IQPKYGVQV LTP+NS
Sbjct: 541 IQPKYGVQVYLTPINS 520

BLAST of Spg038507 vs. ExPASy TrEMBL
Match: A0A0A0L6Y6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 3.0e-219
Identity = 404/556 (72.66%), Postives = 441/556 (79.32%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           MAI+I LTTLLVI +SSLAKIAYDT+SCYWLTPRRIRK M KQGVRGP PRPL+GNILDV
Sbjct: 1   MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
           AAL+ KSTA DMSSIDH+IVDRLLPHYT WTKQY                   +  +  +
Sbjct: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQY------------------GKRFIYWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
           G++ R    + + + E+++  +         +    W +   +       L     +   
Sbjct: 121 GMEPRLCLAETELIKEVLSKNS--------NVCGRSWLQRQGTKHFIGKGLLMANGEDWY 180

Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
              HI     F    L          SYAG MVECT K+I+S E+E DSGRTEFEIADYM
Sbjct: 181 HQRHI-VAPSFMGDKL---------KSYAGLMVECTNKLIQSLETEFDSGRTEFEIADYM 240

Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
           K+LTADIISRTEFD +SDKG QIFHLLTLLQ LCAQASRHLC PGSRFFPSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSL 300

Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKRED---GFSLNLQLIMDE 360
           KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQ++RED   GFSLNLQLIMDE
Sbjct: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQKRREDGDNGFSLNLQLIMDE 360

Query: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNM 420
           CKTFFFAGHETTALLLTWTIMLLATNPNWQ KVR E+NQVC+ G TPS+EHLSK TLLNM
Sbjct: 361 CKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQVCHNGATPSVEHLSKFTLLNM 420

Query: 421 VINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPE 480
           VINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWIPVLAIHHSEELWGKD NEFKPE
Sbjct: 421 VINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPE 480

Query: 481 RFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLT 540
           RFA+KPFSGGRFIPFAAGPRNCIGQ FA+MEAKIILAMLLSKFSF+ISDSYRHAPV VLT
Sbjct: 481 RFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKIILAMLLSKFSFSISDSYRHAPVSVLT 520

Query: 541 IQPKYGVQVCLTPLNS 554
           IQPKYGVQV LTP+NS
Sbjct: 541 IQPKYGVQVYLTPINS 520

BLAST of Spg038507 vs. ExPASy TrEMBL
Match: A0A6J1J2Y1 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 3.5e-207
Identity = 388/554 (70.04%), Postives = 426/554 (76.90%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           MA +IFLTTL VI +SSLAKI+YDT S Y LTPRRIRKTM +QGVRGP+PR LIGNILDV
Sbjct: 1   MAFIIFLTTLFVIFISSLAKISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
             LVAKSTA DMSSIDHDIVDRLLPHYT WTKQY                   +  +  +
Sbjct: 61  VGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQY------------------GKRFIYWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
           G + R    + + + E++  K+         +    W +   +       L     +   
Sbjct: 121 GTEPRLCLAETELIKELLGKKSS--------VCGRSWLQRQGTKHFIGKGLLMANGEDWY 180

Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
              HI     F    L          SYAG+MVECT KMI+S E+ELDSGRTEFEIADYM
Sbjct: 181 HQRHI-VAPAFMGDKL---------KSYAGFMVECTNKMIQSMETELDSGRTEFEIADYM 240

Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
           K+LTADIISRTEFDT SDKG QIFHLLTLLQRLCAQASRHLC PGSRF PSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSKYNREIKSL 300

Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM-QRKREDGFSLNLQLIMDECK 360
           K EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLLNEM QRK     +LNLQLI+DECK
Sbjct: 301 KKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQRKNSTTTTLNLQLIIDECK 360

Query: 361 TFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVI 420
           TFFFAGHETTALLLTWTIMLLATNPNWQH VR E+NQVCN   TPSI+HLSKLTLLNMVI
Sbjct: 361 TFFFAGHETTALLLTWTIMLLATNPNWQHMVRAEVNQVCN-ARTPSIQHLSKLTLLNMVI 420

Query: 421 NESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERF 480
           NESLRLYPPATVLPRMA ED+KLGD++IPKGLSIWIPVLAIHHSEELWGKDAN+F P RF
Sbjct: 421 NESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGKDANQFNPNRF 480

Query: 481 ATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTIQ 540
           A K F GGRFIPFA GPRNCIGQ FA+MEAKIILAM LSKFSFTISD+YRHAPV+VLTIQ
Sbjct: 481 AAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKFSFTISDTYRHAPVVVLTIQ 517

Query: 541 PKYGVQVCLTPLNS 554
           PK+GVQVCLTP +S
Sbjct: 541 PKHGVQVCLTPFSS 517

BLAST of Spg038507 vs. ExPASy TrEMBL
Match: A0A6J1F8S1 (cytokinin hydroxylase OS=Cucurbita moschata OX=3662 GN=LOC111441866 PE=3 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 2.5e-205
Identity = 381/553 (68.90%), Postives = 424/553 (76.67%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           MA +IFLTTLL+I ++SLA+I+YDT S Y LTPRRIRKTM +QGVRGP+PR LIGNILDV
Sbjct: 1   MAFIIFLTTLLLIFLASLARISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
           A LVAKSTA  MSSIDHDIVDRLLPHYT WTKQY                   +  +  +
Sbjct: 61  AGLVAKSTANHMSSIDHDIVDRLLPHYTIWTKQY------------------GKRFIYWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGTS 180
           G + R    + + + E++  K+         +    W +   +       L     +   
Sbjct: 121 GTEPRLCLAETELIKELLGKKSS--------VCGRSWLQRQGTKHFIGKGLLMANGEDWY 180

Query: 181 FPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYM 240
              HI     F    L          SYAG+MVECT KMI+S E+EL+SGRTEFEIADYM
Sbjct: 181 HQRHI-MAPAFMGDKL---------KSYAGFMVECTNKMIQSMETELESGRTEFEIADYM 240

Query: 241 KRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSL 300
           K+LTADIISRTEFDT SDKG QIFHLLTLLQRLCAQASRHLC PGSRF PSKYNREIKSL
Sbjct: 241 KQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQASRHLCLPGSRFVPSKYNREIKSL 300

Query: 301 KMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLNLQLIMDECKT 360
           K EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLLNEMQ+++    +LNLQLIMDECKT
Sbjct: 301 KKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLLNEMQQRKNISTTLNLQLIMDECKT 360

Query: 361 FFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVIN 420
           FFFAGHETTALLLTWTIMLLATNPNWQH VR ++NQVCN    PSI+HLSKLTLLNMVIN
Sbjct: 361 FFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVNQVCN-ARAPSIQHLSKLTLLNMVIN 420

Query: 421 ESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERFA 480
           ESLRLYPPATVLPRMA ED+KLGD++IPKGLSIWIPVLAIHHSEELWGKDAN+F P RF 
Sbjct: 421 ESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIWIPVLAIHHSEELWGKDANQFNPNRFG 480

Query: 481 TKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTIQP 540
            K F GGRFIPFA GPRNCIGQ FA+MEAKIILAM LSKF FTISD+YRHAPV+VLTIQP
Sbjct: 481 AKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILAMFLSKFRFTISDTYRHAPVVVLTIQP 516

Query: 541 KYGVQVCLTPLNS 554
           KYGVQVCLTP  S
Sbjct: 541 KYGVQVCLTPFTS 516

BLAST of Spg038507 vs. ExPASy TrEMBL
Match: A0A5A7V8T6 (Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold508G00410 PE=3 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 8.8e-203
Identity = 373/517 (72.15%), Postives = 407/517 (78.72%), Query Frame = 0

Query: 40  MAKQGVRGPEPRPLIGNILDVAALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSD 99
           M KQGVRGP PRPL+GNILDVAAL+AKSTA DMSSIDH+IVDRLLPHYT WTKQY     
Sbjct: 1   MEKQGVRGPVPRPLVGNILDVAALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQY----- 60

Query: 100 RYRKEVHLLERDGAEAVLDGDGIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGR 159
                         +  +  +G++ R    + + + E+++  +         +    W +
Sbjct: 61  -------------GKRFIYWNGMEPRLCLAETELIKEVLSKNS--------NVCGRSWLQ 120

Query: 160 LVSSTSHRCTCLYGGQAQGTSFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKM 219
              +       L     +      HI     F    L          SYAG MVECT K+
Sbjct: 121 RQGTKHFIGKGLLMANGEDWYHQRHI-VAPSFMGDKL---------KSYAGLMVECTDKL 180

Query: 220 IESFESELDSGRTEFEIADYMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASR 279
           I+S E+ELDSGRTEFEIADYMK+LTADIISRTEFD +SDKG QIFHLLTLLQ LCAQASR
Sbjct: 181 IQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQASR 240

Query: 280 HLCFPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM 339
           HLC PGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM
Sbjct: 241 HLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEM 300

Query: 340 QRKRED---GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQ 399
           QRK++D   GFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQ KVR E+NQ
Sbjct: 301 QRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHEVNQ 360

Query: 400 VCNGGTTPSIEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIP 459
           VC+ G TPS+EHLSK TLLNMVINESLRLYPPATVLPRMAFEDIK+GDLEIPKGLSIWIP
Sbjct: 361 VCHNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIP 420

Query: 460 VLAIHHSEELWGKDANEFKPERFATKPFSGGRFIPFAAGPRNCIGQAFAMMEAKIILAML 519
           VLAIHHSEELWGKD NEFKPERFA+KPFSGGRFIPFAAGPRNC+GQ FA+MEAKIILAML
Sbjct: 421 VLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKIILAML 480

Query: 520 LSKFSFTISDSYRHAPVIVLTIQPKYGVQVCLTPLNS 554
           LSKFSFTISDSYRHAPV VLTIQPKYGVQV LTP+NS
Sbjct: 481 LSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 481

BLAST of Spg038507 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 601.3 bits (1549), Expect = 7.9e-172
Identity = 318/554 (57.40%), Postives = 393/554 (70.94%), Query Frame = 0

Query: 3   IVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDVAA 62
           ++  L +LLVI V+++ ++ YDT+SCYWLTPRRI+K M +QGV GP+PRPL GNIL+++A
Sbjct: 2   LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISA 61

Query: 63  LVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGDGI 122
           +V++S + D  SI HDIV RLLPHY AW+KQY                   +  +  +G 
Sbjct: 62  MVSQSASKDCDSIHHDIVGRLLPHYVAWSKQY------------------GKRFIVWNGT 121

Query: 123 DKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGK-WGRLVSSTSHRCTCLYGGQAQGTSF 182
           D R    + + + E++    G         V G+ W +   + +     L     Q    
Sbjct: 122 DPRLCLTETELIKELLMKHNG---------VSGRSWLQQQGTKNFIGRGLLMANGQDWHH 181

Query: 183 PPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADYMK 242
             H++    F+   L           YA +MVECT K++E    E+  G  E EI + M 
Sbjct: 182 QRHLA-APAFTGERL---------KGYARHMVECTSKLVERLRKEVGEGANEVEIGEEMH 241

Query: 243 RLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKSLK 302
           +LTADIISRT+F +S +KGK++F+ LT+LQR CAQA+RHLCFPGSRF PSKYNREIKSLK
Sbjct: 242 KLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLCFPGSRFLPSKYNREIKSLK 301

Query: 303 MEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQ-RKREDGFSLNLQLIMDECKT 362
            EVERLL+EIIQSR+D  E+GRS+ +G DLLG+LLNEM   K  +  + NLQLIMDECKT
Sbjct: 302 KEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKT 361

Query: 363 FFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVIN 422
           FFFAGHETTALLLTWT MLLA NP WQ KVR+E+ +V      PS++ LSKLT L+ VIN
Sbjct: 362 FFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVIN 421

Query: 423 ESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERFA 482
           ESLRLYPPAT+LPRMAFED+KLGDL IPKGLSIWIPVLAIHHSEELWGKDAN+F PERF 
Sbjct: 422 ESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERFG 481

Query: 483 TKPFSGGR-FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTIQ 542
            +PF+ GR FIPFAAGPRNCIGQ FA+MEAKIILA L+SKF+FTIS +YRHAP++VLTI+
Sbjct: 482 GRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIK 518

Query: 543 PKYGVQVCLTPLNS 554
           PKYGVQV L PL S
Sbjct: 542 PKYGVQVILKPLVS 518

BLAST of Spg038507 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 587.4 bits (1513), Expect = 1.2e-167
Identity = 315/554 (56.86%), Postives = 387/554 (69.86%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           M + + L  +LVI+++ + ++ YD++ CY+LTPRRI+K M +QG+ GP+PR L GNI+D+
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
           + +++ S + D SSI H+IV RLLPHY +W+KQY                   +  +  +
Sbjct: 61  SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQY------------------GKRFIMWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGK-WGRLVSSTSHRCTCLYGGQAQGT 180
           G + R       C+ E    K   + L +   V GK W +   +       L     +  
Sbjct: 121 GTEPRL------CLTETEMIK---ELLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAW 180

Query: 181 SFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEFEIADY 240
               H++    F+   L           YA +MVECTK M E    E+     E EI + 
Sbjct: 181 HHQRHMA-APAFTRDRL---------KGYAKHMVECTKMMAERLRKEVGE---EVEIGEE 240

Query: 241 MKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIKS 300
           M+RLTADIISRTEF +S DKGK++F LLT+LQRLCAQA+RHLCFPGSRF PSKYNREIKS
Sbjct: 241 MRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPGSRFLPSKYNREIKS 300

Query: 301 LKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLNLQLIMDECK 360
           LK EVERLLMEII SRKD VEIGRS+ YG DLLG+LLN+M   +    +LN+Q+IMDECK
Sbjct: 301 LKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNKN---NLNVQMIMDECK 360

Query: 361 TFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTLLNMVI 420
           TFFF GHETT+LLLTWT+MLLA NP WQ  VRDE+ QVC     PS+E LS LT LN VI
Sbjct: 361 TFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVI 420

Query: 421 NESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERF 480
           NESLRLYPPAT+LPRMAFEDIKLGDL IPKGLSIWIPVLAIHHS ELWG+DANEF PERF
Sbjct: 421 NESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERF 480

Query: 481 ATKPFSGGR-FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRHAPVIVLTI 540
            T+ F+  R F+PFAAGPRNCIGQ FAMMEAKIILAML+SKFSF IS++YRHAP++VLTI
Sbjct: 481 TTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTI 511

Query: 541 QPKYGVQVCLTPLN 553
           +PKYGVQ+ L PL+
Sbjct: 541 KPKYGVQLVLKPLD 511

BLAST of Spg038507 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 288.5 bits (737), Expect = 1.1e-77
Identity = 193/566 (34.10%), Postives = 289/566 (51.06%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           M +VIFL  +++IL+  L +I    +   W  P  + + +  QG+ GP  R   GN+ ++
Sbjct: 1   MGLVIFLALIVLILIIGL-RIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSEI 60

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
             +  +S    +    +DI  R+LPHY  W  QY                   E  L  +
Sbjct: 61  KKMKRESHLSILDPSSNDIFPRILPHYQKWMSQY------------------GETFLYWN 120

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSH-RCTCLYGGQA--- 180
           G + R       C+ +   AKT         ++  K G  V S +      L G +    
Sbjct: 121 GTEPRI------CISDPELAKT---------MLSNKLGFFVKSKARPEAVKLVGSKGLVF 180

Query: 181 -QGTSFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTEF- 240
            +G  +  H   L    S   L              MV+CT KM+E +  E     TE  
Sbjct: 181 IEGADWVRHRRILNPAFSIDRLKI--------MTTVMVDCTLKMLEEWRKESTKEETEHP 240

Query: 241 ----EIADYMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFP 300
               E+ +  +RLTADII+ + F +S  +G ++F     L+R    +   +  PG+++ P
Sbjct: 241 KIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELKRCYTTSLNQVSIPGTQYLP 300

Query: 301 SKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLN 360
           +  N  +  L+ +++  +  II SR     +   + YG DLLG+LL     + ++   ++
Sbjct: 301 TPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDDLLGILLKAYNTEGKER-KMS 360

Query: 361 LQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLS 420
           ++ I+ EC+TFFF GHETT+ LL WT MLL+ + +WQ K+R+E+ + C    TP  E  S
Sbjct: 361 IEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECGKEKTPDSETFS 420

Query: 421 KLTLLNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKD 480
           KL L+NMVI ESLRLY P + L R A  +IKLGDLEIPKG ++ IP+L +H  + LWG D
Sbjct: 421 KLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPKGTTVVIPLLKMHSDKTLWGSD 480

Query: 481 ANEFKPERFATKPFSGGR----FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSF-TIS 540
           A++F P RFA             + F+ GPR CIGQ F M+EAK +L M+L +F F ++ 
Sbjct: 481 ADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFVMIEAKTVLTMILQRFRFISLC 517

Query: 541 DSYRHAPVIVLTIQPKYGVQVCLTPL 552
           D Y+H PV  +TIQP+YG+ V L PL
Sbjct: 541 DEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Spg038507 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 280.0 bits (715), Expect = 4.0e-75
Identity = 194/562 (34.52%), Postives = 277/562 (49.29%), Query Frame = 0

Query: 1   MAIVIFLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDV 60
           M ++  +  L + LV  +    Y         P  + +   KQG+ GP+ R L GN+ ++
Sbjct: 56  MELLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREI 115

Query: 61  AALVAKSTAGDMSSIDHDIVDRLLPHYTAWTKQYAFGSDRYRKEVHLLERDGAEAVLDGD 120
             +  ++    +    +DIV R+LPH   W  QY                   E  L   
Sbjct: 116 RKMKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQY------------------GETFLYWQ 175

Query: 121 GIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQ---AQ 180
           G D R       C+ +   AK   Q L  + +   K     S T      L G       
Sbjct: 176 GTDPRL------CISDHELAK---QILSNKFVFFSK-----SKTKPEILKLSGNGLIFVN 235

Query: 181 GTSFPPHISYLF-VFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESELDSGRTE--F 240
           G  +  H   L   FS   L               MV+CT +M   ++ + +   TE   
Sbjct: 236 GLDWVRHRRILNPAFSMDKL---------KLMTQLMVDCTFRMFLEWKKQRNGVETEQFV 295

Query: 241 EIADYMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYN 300
            I+   KRLTADII+   F +S  +G ++F     LQ+ CA A   L FPG ++ P+  N
Sbjct: 296 LISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSN 355

Query: 301 REIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDGFSLNLQLI 360
            +I  L M+V   +  II +R        S  YG+DLLG++L       E    +++  I
Sbjct: 356 LQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIMLTAAS-SNESEKKMSIDEI 415

Query: 361 MDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTL 420
           ++ECKTFFFAGHETTA LLTW+ MLL+ + +WQ K+R+E+   C     P  E  SKL L
Sbjct: 416 IEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKL 475

Query: 421 LNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEF 480
           +N V  ESLRLY P   L R+A ED+KLG+LEIPKG +I +P+  +H  + +WG DA++F
Sbjct: 476 MNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDADKF 535

Query: 481 KPERFATKPFSGGR----FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRH 540
            P RFA             + F+ GPR CIGQ FA+MEAK +LAM+L +F   +S  Y+H
Sbjct: 536 NPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADYKH 571

Query: 541 APVIVLTIQPKYGVQVCLTPLN 553
           AP   LT+QP+Y + V L P++
Sbjct: 596 APADHLTLQPQYDLPVILEPID 571

BLAST of Spg038507 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 5.3e-75
Identity = 180/563 (31.97%), Postives = 294/563 (52.22%), Query Frame = 0

Query: 6   FLTTLLVILVSSLAKIAYDTLSCYWLTPRRIRKTMAKQGVRGPEPRPLIGNILDVAALVA 65
           F+  +LV+ V  L+ +    +S  W  PR+I +  +KQG+RGP     IGN+ ++  ++ 
Sbjct: 9   FIPKVLVLSV-ILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMML 68

Query: 66  KSTAGDMSSIDHDIVDRLLPHYTAWTKQYA------FGSDRYRKEVHLLERDGAEAVLDG 125
           K+++  M    H+I+ R+L  Y  W K Y       FG   +R  V       A+  L  
Sbjct: 69  KASSHPM-PFSHNILPRVLSFYHHWRKIYGATFLVWFG-PTFRLTV-------ADPDLIR 128

Query: 126 DGIDKRTIGKKQQCVWEIVAAKTGNQTLHRERIVDGKWGRLVSSTSHRCTCLYGGQAQGT 185
           +   K    +K +    +V    G+  L    +   KW        HR            
Sbjct: 129 EIFSKSEFYEKNE-AHPLVKQLEGDGLL---SLKGEKWAH------HR------------ 188

Query: 186 SFPPHISYLFVFSSSSLLSADYDFFDNSYAGYMVECTKKMIESFESEL-DSGRTEFEIAD 245
                IS  F   +  LL              +++    M++ +  +L ++G  E ++ +
Sbjct: 189 ---KIISPTFHMENLKLL-----------VPVVLKSVTDMVDKWSDKLSENGEVEVDVYE 248

Query: 246 YMKRLTADIISRTEFDTSSDKGKQIFHLLTLLQRLCAQASRHLCFPGSRFFPSKYNREIK 305
           + + LT D+ISRT F +S + G+ +F L      LCA+A + +  PG RFFP++ N +  
Sbjct: 249 WFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYRFFPTRGNLKSW 308

Query: 306 SLKMEVERLLMEIIQSRKDGVEIGR-STC---YGSDLLGMLLNEMQRKREDGFSLNLQLI 365
            L  E+ + L+++I+ R+     G    C      DLLG+++   Q K     ++ +Q I
Sbjct: 309 KLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI---QAK-----NVTVQDI 368

Query: 366 MDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRDELNQVCNGGTTPSIEHLSKLTL 425
           ++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K RDE+ +VC     P+ +H+ KL  
Sbjct: 369 VEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKT 428

Query: 426 LNMVINESLRLYPPATVLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEF 485
           L+M++NESLRLYPP     R A  D+KLG  +IP G  + IP++A+HH + +WG D NEF
Sbjct: 429 LSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEF 488

Query: 486 KPERFATKPFSGGR----FIPFAAGPRNCIGQAFAMMEAKIILAMLLSKFSFTISDSYRH 545
            P RFA       +    FIPF  G R CIGQ  A+++AK+ LA+++ +F+F ++ +Y+H
Sbjct: 489 NPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQH 517

Query: 546 APVIVLTIQPKYGVQVCLTPLNS 554
           AP +++ + P++G  +    L +
Sbjct: 549 APTVLMLLYPQHGAPITFRRLTN 517

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038905687.16.0e-22274.59cytokinin hydroxylase-like [Benincasa hispida][more]
XP_008461587.13.9e-22173.20PREDICTED: cytokinin hydroxylase-like [Cucumis melo][more]
XP_004145660.16.2e-21972.66cytokinin hydroxylase [Cucumis sativus] >KGN57750.1 hypothetical protein Csa_009... [more]
XP_022983651.17.1e-20770.04cytokinin hydroxylase-like [Cucurbita maxima][more]
XP_023528555.12.3e-20568.76cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FF181.1e-17057.40Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1[more]
Q9ZW951.7e-16656.86Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1[more]
Q9ASR31.6e-7634.10Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1[more]
Q6Z6D64.4e-7432.33Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 ... [more]
F4IK455.7e-7434.52Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CGC01.9e-22173.20cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1[more]
A0A0A0L6Y63.0e-21972.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1[more]
A0A6J1J2Y13.5e-20770.04cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1[more]
A0A6J1F8S12.5e-20568.90cytokinin hydroxylase OS=Cucurbita moschata OX=3662 GN=LOC111441866 PE=3 SV=1[more]
A0A5A7V8T68.8e-20372.15Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
Match NameE-valueIdentityDescription
AT5G38450.17.9e-17257.40cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT1G67110.11.2e-16756.86cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT2G46960.21.1e-7734.10cytochrome P450, family 709, subfamily B, polypeptide 1 [more]
AT2G46950.14.0e-7534.52cytochrome P450, family 709, subfamily B, polypeptide 2 [more]
AT2G26710.15.3e-7531.97Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 297..317
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 4..100
coord: 206..547
NoneNo IPR availablePANTHERPTHR24282:SF186CYTOCHROME P450 FAMILY PROTEINcoord: 206..547
NoneNo IPR availablePANTHERPTHR24282:SF186CYTOCHROME P450 FAMILY PROTEINcoord: 4..100
IPR001128Cytochrome P450PRINTSPR00385P450coord: 417..428
score: 46.75
coord: 490..499
score: 57.09
coord: 499..510
score: 43.74
coord: 364..381
score: 40.88
IPR001128Cytochrome P450PFAMPF00067p450coord: 207..526
e-value: 2.2E-73
score: 247.6
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 457..481
score: 24.84
coord: 499..522
score: 33.64
coord: 373..399
score: 29.51
coord: 489..499
score: 52.03
coord: 353..370
score: 29.73
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 187..548
e-value: 4.3E-94
score: 317.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 193..550

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg038507.1Spg038507.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen