Spg038463 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg038463
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionsirohydrochlorin ferrochelatase, chloroplastic
Locationscaffold12: 1196107 .. 1200877 (+)
RNA-Seq ExpressionSpg038463
SyntenySpg038463
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTCTAAAGCTCCAGGAATCAGATCTAGGTTCTTAGTCCTTGATATGAGCCCTATTTACAAGGTGCAGAATGCTTTTTTGCAGGCTAACTTAACTAAACTCAAGATTTACCTAGCCATCAAAAGCCAGACTAGTACATGGCTCAATTCTTTGAGAACTGTTGGAACATCCTCAAAAGCAACATCTTGATATTGTTCCTGGGTTTTTTCTAAAAGTTGTTGCGAATGCCTTGGTCAATGAAACCTACAGTTCACATGTATTATCCCTTAGGATTCAGGTGCTAAACGAGTTAGCAAGTTTAGACCAACTAGTCTTACTACAGGCGTTTATAAGCTGATCATTGAAGTTCTTGCCGAACGTGTTATTACAAGGTGTTGCCAATCACCATTACTAATCTTTAATTAACTTTCCTAGGTGGCAAAAAATTATTAGTTCGTGTTGTTTTGTTAATGAGCTGATCGATGATTGGAAAAGCAAAGAGAAAGAAGGATTGATTCTTGAACTAGATTTTGAAAATGCCTTTGATTGAGTTGATTATTGTCTCTATTAGGTATCCAAACTGTAACGTCGCCAATTTAAAGTAAGCTCTTATTTCTCAAATTACAAGTGTAATTGAGAAAATTTTCAATTAATTGGTTGAATTAAAAGTTCAATTAAAAGAGTTAATTGTCTTGGAAAAAAAGATTGGATAAGCTTGATGACAATTTTGCAATTCAAGTAAGTTGGATGGTGATTTTGTAAATAAGTTGGCTAGTAACCAAGCCAAGCCAAACCCTAATACTCTTTTAAGCTCAATGCACGCAGCCCAAGGAGATTAAAAAAAAAAAAGAGTTTCATCTTCTTTCTCCATTTCCTTCTCCTTCAACCAAAAGACCCATCCTCCATAGCCTTTTCTTCTTCTTTTGCCATAGCCATTGGCCACACCGTTCTCCTCCATCACCGATTGTCTTCTCCGACAACAGCAAAACAGAGGCGGTGGTGGTTTCCGAGCAGTCCTTCGCGTGGCCAACAACGTCTTGTGCGTGGTTAAGTGTTTTCTTTCCTCGGTTTTGGTTGTTTCTCAAGCCGATCTCTCTCCCTTTTCGTTCTCTCTCACTTTTGATCTCTCCGTCTCTGTTTTCTGGCGGATTTTAGGCGTGGTAGTGGCTGGTTTCACGTTTTTTCCGGCGGGTACAGTGTAGGCGCGTGATCAGCAGCAGTTTGAGGCGTTTTTTTTAGTGTTTTTGGCGAGTTTTCAACATGGGTAAGTGAGATTTAAGGTGTTTTTGATCAATTTTATGTTTAATCAAGGATACCATGGATGTTAGCTCTCAATTTCAAGGTACCCATAGCGTTTAGTGATATATTCGTCAATTTGGAGTGAAATTTAAAGTTTGGACAGCAAGCATTTGTTCTGTTTTTGGGTAAGTTTTAGGTGTTAAATTCCCCAAATTTAATGAGCTCCTAGTAGAACAATTGATTTGAAATTCTATTTGGTTTTGAGGAAGTTTTCCCTTTTTGTGTAGCTTGCCTAAGGCCTTGGAGTTAAGGAATTTTGAAAAGGATTTAGCAAATATTGTTTGGCAAAGGTAAGTAGCAATTTGGAATAAGTTCTATGCTTTAAACCCATTTTATTTGAGAATGGTTTATGCTAGTATGAATTAGATTTAATCTTTAAGTTGGCCTTTGAATTTAAGTAGCATATTGAGTTAATTTTGGAGCATGATGTTAGTATGAAATTTTAAGGTTTATTTATGATTACATGAAAAGTATGTTGAGATTTGCCTTTAGTTGGGCGTGGTGCGAGTAGCATGGATTATGGGTTAATTGAGTTTGGGTTGAATTATTATTAATTGTAAAGCCTGCTTGTTATGTGAGAATGAAATTTCATGTTCTATTTGGGTTAGTCATGAAGTAATTTGCTTTGAATGAATTTATGTTCGGCTGATGATCTCTAAAGTGGTAGTAGATATGATTGAGCATGCAAAATGTGGACTTTGTACTGGAATGTTAATGGCTAAAATTACTCTAGCTTAGAAGTGTGATTTTTTGCTTTTCAGGTGACTACAATTTTTAGGATAGTTGTAGTTACGTCGTTATGCTGTCGAATTTTCGGTAGCTTTATAGTAGTTATCGGGGCTAAGTTTAACAATGTCCTTTGGATTATGATGTGCTGATATTGACTGGCCGATTAGTATAGACCACAGAATATCCAAACATGTTTTAGTATGACTTATTGAGGACCATATGAATGAATGGGAATAGGCGTAGAATAGGGACCATAACATGTTTTAGGGCCTTAGGCAGACTTAAAGGATCCTTAAGTCTTATGTTAACTAGAGTGGTTTCCTTCCCCATCTCAGGGCTGATAGAACCCAATTTTTAGGCACGTTATCGTGTGTTCTATCAACTGACTGTTAGAGCCCAATTACAGGCACGTTATCGTGCGCTCTCAGCATGTAATTGTTTTGATTGTCTTATTTAAATTTGTTGATTTTTTTTCCCTCTTACTCTTTTACCTTTGATTTCAGGAAGAAGTTAACTAAATTTACTTCATAGTATACTCTCATGACTCAGCACGTGAAATCTAGATCAAGCATTAGAGTCATTGCGTTGAAATGATTAGATTTCTAAAATAAAGATCAATTTTACAATTTTCAATAAAAACATGTTAAGTTTGTAAAAACCACAAATTTTCTTTTATAGCCCACCACCCAGGCAACCACCAAGTATTTCAAACGACGTCGTTTGCCAAGACAATCCTTCCCTTCAGGTTCAGAAGGCTATCTTCGATCGGAACTCATGAGATTGTTATCCATCACTCCTCAAATTACAGTAAAGAGGTCTGAAAATCTTCTTAACTCCCCGTTTTTCAATTTGCTTAATCTGCCTCTAATGGTTGTTCCTCCATTGAAGTCCCTCAGCAAGCGAAACTAGAAGGACATTCACAATCAGCGCATCTCAGAAATCTCCGATTCTGATCGGACGGCCATCGAGATTCAAGAATTTCACATTGCGGTTCCGCATGAGTGTCGATAATGGAAGCCCTAATCGGAGTCGAGATCGGATCGGTGCCGGAGATGCCGTCATCATCGTCGACCATGGCTCTCGCCGCCAGGAATCTAATCTGATGCTGAGTAAGCCAAGATTTTGGAATTTGGAATTTGGAATTAGGGTGAAATTTGAAGTTCTACGTTGGCTAATCCTCTTGTGGTTCTGAATTTGTTCGAAAATTTCAGATGAGTTCGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAGCCTGCTCACATGGTATGTTCTTCTTAACTGTATTTTTTTTTTGAGAAAATATTCTTAACTCTATTTTTCACTTGGTTGAGCTACTAATGTCATCCCTATAGCTTGATATTTATTTAATTTTGAGTTTTAAAACGTTTGAATTATTGCTTGAAATTTGAATTATTTTTATCTTTTGTCTCTATCTTGTAACAGCAATTAATGAAAGACTAACGCTGCATTTTATTTGATAAAATTTAAACGAGATCCAATATTTATTTATTTAATAAAATGTCATATTAACATTTGACTAGTTGATGTTAACATTAGGACTTGAAATAAACCACAATTCGCTAATTACGTAATCAAAAACTTTTTGATGTCAAACTAATTTTTCAGTTTGGTGGATGATCTTTCCTTCCAATTAAACGAGATTATTTTGGAGGCTTTATTGGCCAAATTGAAGTGTTTGCCTTATTGTACTTTAGTTTTTCTTCCTTCCCCACCCATTAAAAAGTGAGAGATTGATTTTTTTGTAAAAAAAAACTTTTTGGAAATATTTCATGTGCCACTCTAAGGACGGACTAAAATGTCGATATTAAAGAATGTCTTTGATATTTTTCATACTTCATTGATATATTTACCATATTATCAAAAATACCAATCATCCTCAATATCAAGGTTGAATCTTTTGGTTTATGAAAATATTGATAGGTTGAGGGGTATATTTCTTTTACTACTCTTTGAAGTACAACATCTCTAGCCTTCAAAATACCATAGGATTCAGTGAATACAAAACCAAAATAAATGATGATATTCGGAAATATGAACAGTGGTATGGAACTATTCCATGGAATCGTACCACTAGAAGTTTCTTTGTCTTTTTCATTCCTTCCAGGCTCTTCCCTTTACACATATGTTTCAACACATGATCTTAGCTATGTAAGTACTGCAGGAGGGGCTCTAAAGATTTATCTTTTACTTTGTTCCAAAACAACACAGGAGCTGGCCGAACCGTCGATAAGAGACTCGTTTGCATCATGTGTCGAACAAGGGGCCAAACGAATCATTGTAAGCCCATTCTTCCTCTTCCCTGGTCGACATTGGCAACAGGTAACTCACTGTAAGATGAATGAACTTAATTGGTGGCCTTTGGATCTTCTGACTCTTAAGTAGGAGGATTGATAACAATGGCTGAAATTTTGCAGGACATTCCTTCCTTAACAGCTGAAGCAGCAAAGGAGCACCCTGGAATATCATATATAATAACTGCTCCTCTTGGTCTTCATGAACAACTAGTGGTAGGAATTTCTCAACTACATTTTTTTTTCCAGTAGAGGATAGTAAAGAAAGCAATGTCATAAATGATAAAAGGAAAAGACTGTATTTGTTAGTATTGAAATGTGAGGTTTTCTGTTGGCAGGATGTTGTGAATGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTTGCAGATGAATGTGAAGTTTGCGCTGGAACAGGCAAATGCAGATTTAATTAG

mRNA sequence

ATGATTCTAAAGCTCCAGGAATCAGATCTAGGTGCTAAACGAGTTAGCAAGTTTAGACCAACTAGTCTTACTACAGGCGTTTATAAGCTGATCATTGAAGTTCTTGCCGAACGTGTTATTACAAGGTATCCAAACTGTAACGTCGCCAATTTAAAATTGGATAAGCTTGATGACAATTTTGCAATTCAACCATTGGCCACACCGTTCTCCTCCATCACCGATTGTCTTCTCCGACAACAGCAAAACAGAGGCGGTGGTGGTTTCCGAGCAGTCCTTCGCGTGGCCAACAACGTCTTGTGCGTGGTTAAGTGTTTTCTTTCCTCGGTTTTGGTTGTTTCTCAAGCCGATCTCTCTCCCTTTTCCTTGCCTAAGGCCTTGGAGTTAAGGAATTTTGAAAAGGATTTAGCAAATATTGTTTGGCAAAGGGCTGATAGAACCCAATTTTTAGGCACGTTATCGTGTTCAGAAGGCTATCTTCGATCGGAACTCATGAGATTGTTATCCATCACTCCTCAAATTACAGTAAAGAGTCCCTCAGCAAGCGAAACTAGAAGGACATTCACAATCAGCGCATCTCAGAAATCTCCGATTCTGATCGGACGGCCATCGAGATTCAAGAATTTCACATTGCGGTTCCGCATGAGTGTCGATAATGGAAGCCCTAATCGGAGTCGAGATCGGATCGGTGCCGGAGATGCCGTCATCATCGTCGACCATGGCTCTCGCCGCCAGGAATCTAATCTGATGCTGAATGAGTTCGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAGCCTGCTCACATGGAGCTGGCCGAACCGTCGATAAGAGACTCGTTTGCATCATGTGTCGAACAAGGGGCCAAACGAATCATTGTAAGCCCATTCTTCCTCTTCCCTGGTCGACATTGGCAACAGGACATTCCTTCCTTAACAGCTGAAGCAGCAAAGGAGCACCCTGGAATATCATATATAATAACTGCTCCTCTTGGTCTTCATGAACAACTAGTGGATGTTGTGAATGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTTGCAGATGAATGTGAAGTTTGCGCTGGAACAGGCAAATGCAGATTTAATTAG

Coding sequence (CDS)

ATGATTCTAAAGCTCCAGGAATCAGATCTAGGTGCTAAACGAGTTAGCAAGTTTAGACCAACTAGTCTTACTACAGGCGTTTATAAGCTGATCATTGAAGTTCTTGCCGAACGTGTTATTACAAGGTATCCAAACTGTAACGTCGCCAATTTAAAATTGGATAAGCTTGATGACAATTTTGCAATTCAACCATTGGCCACACCGTTCTCCTCCATCACCGATTGTCTTCTCCGACAACAGCAAAACAGAGGCGGTGGTGGTTTCCGAGCAGTCCTTCGCGTGGCCAACAACGTCTTGTGCGTGGTTAAGTGTTTTCTTTCCTCGGTTTTGGTTGTTTCTCAAGCCGATCTCTCTCCCTTTTCCTTGCCTAAGGCCTTGGAGTTAAGGAATTTTGAAAAGGATTTAGCAAATATTGTTTGGCAAAGGGCTGATAGAACCCAATTTTTAGGCACGTTATCGTGTTCAGAAGGCTATCTTCGATCGGAACTCATGAGATTGTTATCCATCACTCCTCAAATTACAGTAAAGAGTCCCTCAGCAAGCGAAACTAGAAGGACATTCACAATCAGCGCATCTCAGAAATCTCCGATTCTGATCGGACGGCCATCGAGATTCAAGAATTTCACATTGCGGTTCCGCATGAGTGTCGATAATGGAAGCCCTAATCGGAGTCGAGATCGGATCGGTGCCGGAGATGCCGTCATCATCGTCGACCATGGCTCTCGCCGCCAGGAATCTAATCTGATGCTGAATGAGTTCGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAGCCTGCTCACATGGAGCTGGCCGAACCGTCGATAAGAGACTCGTTTGCATCATGTGTCGAACAAGGGGCCAAACGAATCATTGTAAGCCCATTCTTCCTCTTCCCTGGTCGACATTGGCAACAGGACATTCCTTCCTTAACAGCTGAAGCAGCAAAGGAGCACCCTGGAATATCATATATAATAACTGCTCCTCTTGGTCTTCATGAACAACTAGTGGATGTTGTGAATGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTTGCAGATGAATGTGAAGTTTGCGCTGGAACAGGCAAATGCAGATTTAATTAG

Protein sequence

MILKLQESDLGAKRVSKFRPTSLTTGVYKLIIEVLAERVITRYPNCNVANLKLDKLDDNFAIQPLATPFSSITDCLLRQQQNRGGGGFRAVLRVANNVLCVVKCFLSSVLVVSQADLSPFSLPKALELRNFEKDLANIVWQRADRTQFLGTLSCSEGYLRSELMRLLSITPQITVKSPSASETRRTFTISASQKSPILIGRPSRFKNFTLRFRMSVDNGSPNRSRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVADECEVCAGTGKCRFN
Homology
[Close]
BLAST of Spg038463 vs. NCBI nr
Match: XP_038890885.1 (sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 379.4 bits (973), Expect = 3.5e-101
Identity = 194/229 (84.72%), Postives = 208/229 (90.83%), Query Frame = 0 

Query: 145 RTQFLGTLSCSEGYLRSELMRLLSI-TPQITVKSPSASETRRTFTISASQKSPILIGRPS 204
           +++ L +LS  +G   SE M  LSI TPQI++KS SA ETRR +TI ASQKSPILI R S
Sbjct: 21  KSRRLASLSLCKG---SEPMTSLSIVTPQISLKSSSAVETRRKYTICASQKSPILIRRLS 80

Query: 205 RFKNFTLRFRMSVDNGSPNRSRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGY 264
           RF+NFT RFRMSVDNGS NRS DRIGAGDAVIIVDHGSRR+ESNLMLNEFVAMFRDKTGY
Sbjct: 81  RFRNFTSRFRMSVDNGSSNRSGDRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGY 140

Query: 265 PIVEPAHMELAEPSIRDSFASCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPG 324
           PIVEPAHMELAEPSIRDSFA CVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAK+HPG
Sbjct: 141 PIVEPAHMELAEPSIRDSFALCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPG 200

Query: 325 ISYIITAPLGLHEQLVDVVNDRINHCLSHAAGVADECEVCAGTGKCRFN 373
           ISYIITAPLGLHE+LVDVVNDRI+HCLSHAAGVA+ECEVCAGTGKCRFN
Sbjct: 201 ISYIITAPLGLHEKLVDVVNDRISHCLSHAAGVAEECEVCAGTGKCRFN 246

[Close]
BLAST of Spg038463 vs. NCBI nr
Match: KAG7033101.1 (Sirohydrochlorin ferrochelatase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 372.1 bits (954), Expect = 5.7e-99
Identity = 185/210 (88.10%), Postives = 195/210 (92.86%), Query Frame = 0 

Query: 164 MRLLSITPQITVKSPSASET-RRTFTISASQKSPILIGRPSRFKNFTLRFRMSVDNGSPN 223
           MR LSITPQITVKS SA +  RRT+TIS SQKS I IGRPSRF NFT RFRMSVDNG  +
Sbjct: 1   MRSLSITPQITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSS 60

Query: 224 RSRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF 283
           +SRDRIGAGDAVIIVDHGSRRQESNLMLN FVAMFRDKTGYPIVEPA MELA+PSIRD+F
Sbjct: 61  QSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAF 120

Query: 284 ASCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVV 343
            SCVEQGAKRII+SPFFLFPGRHWQQDIPSLTAEAAK+HPGISYI+TAPLGLHE+LVDVV
Sbjct: 121 KSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180

Query: 344 NDRINHCLSHAAGVADECEVCAGTGKCRFN 373
           NDRINHCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 NDRINHCLSHAAGVADECEVCAGTGKCRFN 210

[Close]
BLAST of Spg038463 vs. NCBI nr
Match: XP_023548507.1 (sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 366.7 bits (940), Expect = 2.4e-97
Identity = 182/210 (86.67%), Postives = 193/210 (91.90%), Query Frame = 0 

Query: 164 MRLLSITPQITVKSPSASET-RRTFTISASQKSPILIGRPSRFKNFTLRFRMSVDNGSPN 223
           MR LSITPQITVKS S+ +T RRT+TIS SQKS I IGR  RF N T RFRMSVDNG  +
Sbjct: 1   MRSLSITPQITVKSFSSGDTRRRTYTISTSQKSAIPIGRHLRFGNSTSRFRMSVDNGGSS 60

Query: 224 RSRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF 283
           RSRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRD TGYPIVEPAHMELA+PSIRD+F
Sbjct: 61  RSRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDNTGYPIVEPAHMELAKPSIRDAF 120

Query: 284 ASCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVV 343
            SCVEQGA RII+SPFFLFPGRHWQQDIPSLTAEAAK+HPGISYI+TAPLGLHE+LVDVV
Sbjct: 121 KSCVEQGANRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180

Query: 344 NDRINHCLSHAAGVADECEVCAGTGKCRFN 373
           NDR+NHCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 NDRMNHCLSHAAGVADECEVCAGTGKCRFN 210

[Close]
BLAST of Spg038463 vs. NCBI nr
Match: XP_022993758.1 (sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 363.6 bits (932), Expect = 2.0e-96
Identity = 182/211 (86.26%), Postives = 192/211 (91.00%), Query Frame = 0 

Query: 164 MRLLSITPQITVKSPSASETRRTFTISASQKSPIL--IGRPSRFKNFTLRFRMSVDNGSP 223
           M  LSI PQITVKSPSA ETR+T+TI ASQKS IL  IGR S  +NF+ RFRMSVDNG  
Sbjct: 1   MTSLSIPPQITVKSPSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGS 60

Query: 224 NRSRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDS 283
           N    RIGAGDAVIIVDHGSRR+ESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDS
Sbjct: 61  N----RIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDS 120

Query: 284 FASCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDV 343
           FASCVEQGAKRI+VSPFFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHEQLVDV
Sbjct: 121 FASCVEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDV 180

Query: 344 VNDRINHCLSHAAGVADECEVCAGTGKCRFN 373
           VNDRI+HCLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 VNDRISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of Spg038463 vs. NCBI nr
Match: KAA0037556.1 (sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa])

HSP 1 Score: 363.6 bits (932), Expect = 2.0e-96
Identity = 180/209 (86.12%), Postives = 190/209 (90.91%), Query Frame = 0 

Query: 164 MRLLSITPQITVKSPSASETRRTFTISASQKSPILIGRPSRFKNFTLRFRMSVDNGSPNR 223
           M  LS+TPQI+ KSPS  ETR  +TI A Q+SPI IGR S+F+NFT R RMS+DNGS N 
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSN- 60

Query: 224 SRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFA 283
              RIGAGDAVIIVDHGSRR+ESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF 
Sbjct: 61  ---RIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFT 120

Query: 284 SCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVN 343
           SCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHE+LVDVVN
Sbjct: 121 SCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVN 180

Query: 344 DRINHCLSHAAGVADECEVCAGTGKCRFN 373
           DRINHCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 DRINHCLSHAAGVADECEVCAGTGKCRFN 205

[Close]
BLAST of Spg038463 vs. ExPASy Swiss-Prot
Match: Q84JH7 (Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIRB PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 2.6e-62
Identity = 110/146 (75.34%), Postives = 126/146 (86.30%), Query Frame = 0 

Query: 225 RDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFAS 284
           ++ IG  D +IIVDHGSRR+ESNLML EFV MF++KTGYPIVEPAHMELAEPSI+D+F+ 
Sbjct: 75  KNGIGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSL 134

Query: 285 CVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVND 344
           CV+QGAKR++VSPFFLFPGRHW  DIPSLTA+AAKE  GISY+ITAPLG H  L+DVVND
Sbjct: 135 CVQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVND 194

Query: 345 RINHCLSHAAGVADECEVCAGTGKCR 371
           RI HCLSH  G ADEC VCAGT KC+
Sbjct: 195 RIQHCLSHVEGDADECLVCAGTNKCK 220

[Close]
BLAST of Spg038463 vs. ExPASy Swiss-Prot
Match: P61817 (Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 6.2e-16
Identity = 41/116 (35.34%), Postives = 62/116 (53.45%), Query Frame = 0 

Query: 232 DAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 291
           DAV+ V HGSR +E       F+   +     PI E   +ELA P+I   F +C+EQGA 
Sbjct: 13  DAVLYVCHGSRVKEGADQAVAFIERCKKNLDVPIQEVCFLELASPTIEQGFEACIEQGAT 72

Query: 292 RIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVNDRIN 348
           RI + P  L    H + DIP    +  + +P +  +   P G+ E++VD++ +RIN
Sbjct: 73  RIAIVPLLLLTAAHAKHDIPEEIQKVYERYPQVEVLYGEPFGVDERIVDILVERIN 128

[Close]
BLAST of Spg038463 vs. ExPASy Swiss-Prot
Match: P61819 (Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=cbiX PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 2.2e-13
Identity = 41/141 (29.08%), Postives = 67/141 (47.52%), Query Frame = 0 

Query: 232 DAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 291
           +A+++V HGSR   S  ++ E     + +  Y IVE   ME  EP+I  +    ++ GAK
Sbjct: 2   EALVLVGHGSRLPHSKNVVMEVAEKIKARNIYDIVEVGMMEFNEPTIPQTIKKVIDAGAK 61

Query: 292 RIIVSPFFLFPGRHWQQDIPSLTA------EAAKEH-------------------PGISY 348
           ++IV+P FL PG H ++DIP +        E    H                    G+  
Sbjct: 62  KVIVTPVFLAPGNHTERDIPKILGIYEGDDEGGHHHHHDHEHHHHHHDTTAVEIPEGVEL 121

[Close]
BLAST of Spg038463 vs. ExPASy Swiss-Prot
Match: Q58380 (Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=cbiX PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.4e-12
Identity = 45/138 (32.61%), Postives = 67/138 (48.55%), Query Frame = 0 

Query: 232 DAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 291
           +A+++V HGSR   S  +L +     +++  +PIVE   ME +EP+I  +    +EQGAK
Sbjct: 2   EALVLVGHGSRLPYSKELLVKLAEKVKERNLFPIVEIGLMEFSEPTIPQAVKKAIEQGAK 61

Query: 292 RIIVSPFFLFPGRHWQQDIPSLTA------EAAKEH------------------PGISYI 346
           RIIV P FL  G H  +DIP L        E   EH                    +  I
Sbjct: 62  RIIVVPVFLAHGIHTTRDIPRLLGLIEDNHEHHHEHSHHHHHHHHHEHEKLEIPEDVEII 121

[Close]
BLAST of Spg038463 vs. ExPASy Swiss-Prot
Match: A6UWT5 (Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3) OX=419665 GN=cbiX PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.7e-11
Identity = 40/138 (28.99%), Postives = 66/138 (47.83%), Query Frame = 0 

Query: 232 DAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAK 291
           +A++++ HGSR   S  ++ +     ++K  Y IVE   ME  EP+I  +    + +GAK
Sbjct: 2   EALVLLGHGSRLPYSKEIVGKVAEKIKEKNIYDIVEIGMMEFNEPTIPQTINKVIAEGAK 61

Query: 292 RIIVSPFFLFPGRHWQQDIPSLTA--EAAKEH----------------------PGISYI 346
           +II+ P FL  G H ++DIP +    E  + H                       G+  I
Sbjct: 62  KIIIVPVFLAHGNHTKRDIPQILGLIECEEHHHEGEGGHHHHHHHHHGEKIEVPEGVEII 121

[Close]
BLAST of Spg038463 vs. ExPASy TrEMBL
Match: A0A5D3BY55 (Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00520 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 9.7e-97
Identity = 180/209 (86.12%), Postives = 190/209 (90.91%), Query Frame = 0 

Query: 164 MRLLSITPQITVKSPSASETRRTFTISASQKSPILIGRPSRFKNFTLRFRMSVDNGSPNR 223
           M  LS+TPQI+ KSPS  ETR  +TI A Q+SPI IGR S+F+NFT R RMS+DNGS N 
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSN- 60

Query: 224 SRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFA 283
              RIGAGDAVIIVDHGSRR+ESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF 
Sbjct: 61  ---RIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFT 120

Query: 284 SCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVN 343
           SCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHE+LVDVVN
Sbjct: 121 SCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVN 180

Query: 344 DRINHCLSHAAGVADECEVCAGTGKCRFN 373
           DRINHCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 DRINHCLSHAAGVADECEVCAGTGKCRFN 205

[Close]
BLAST of Spg038463 vs. ExPASy TrEMBL
Match: A0A6J1K384 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111489668 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 9.7e-97
Identity = 182/211 (86.26%), Postives = 192/211 (91.00%), Query Frame = 0 

Query: 164 MRLLSITPQITVKSPSASETRRTFTISASQKSPIL--IGRPSRFKNFTLRFRMSVDNGSP 223
           M  LSI PQITVKSPSA ETR+T+TI ASQKS IL  IGR S  +NF+ RFRMSVDNG  
Sbjct: 1   MTSLSIPPQITVKSPSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGS 60

Query: 224 NRSRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDS 283
           N    RIGAGDAVIIVDHGSRR+ESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDS
Sbjct: 61  N----RIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDS 120

Query: 284 FASCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDV 343
           FASCVEQGAKRI+VSPFFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHEQLVDV
Sbjct: 121 FASCVEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDV 180

Query: 344 VNDRINHCLSHAAGVADECEVCAGTGKCRFN 373
           VNDRI+HCLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 VNDRISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of Spg038463 vs. ExPASy TrEMBL
Match: A0A1S3C8S9 (sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498093 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 9.7e-97
Identity = 180/209 (86.12%), Postives = 190/209 (90.91%), Query Frame = 0 

Query: 164 MRLLSITPQITVKSPSASETRRTFTISASQKSPILIGRPSRFKNFTLRFRMSVDNGSPNR 223
           M  LS+TPQI+ KSPS  ETR  +TI ASQ+SPI IGR S+F+NFT R RMS+DNGS N 
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSN- 60

Query: 224 SRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFA 283
              RIGAGDAVIIVDHGSRR+ESN MLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF 
Sbjct: 61  ---RIGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFT 120

Query: 284 SCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVN 343
           SCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHE+LVDVVN
Sbjct: 121 SCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVN 180

Query: 344 DRINHCLSHAAGVADECEVCAGTGKCRFN 373
           DRINHCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 DRINHCLSHAAGVADECEVCAGTGKCRFN 205

[Close]
BLAST of Spg038463 vs. ExPASy TrEMBL
Match: A0A6J1FHT5 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445470 PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 1.1e-95
Identity = 181/211 (85.78%), Postives = 191/211 (90.52%), Query Frame = 0 

Query: 164 MRLLSITPQITVKSPSASETRRTFTISASQKSPILI--GRPSRFKNFTLRFRMSVDNGSP 223
           M  LSI  QITVKSPSA ETR+T+TI ASQKS ILI  GR S  +NF+ RFRMSVDNG  
Sbjct: 1   MTSLSIPSQITVKSPSAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRFRMSVDNG-- 60

Query: 224 NRSRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDS 283
               DRIGAGDAVIIVDHGSRR+ESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDS
Sbjct: 61  --GADRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDS 120

Query: 284 FASCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDV 343
           FASCVEQGAKRI+VSPFFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHEQLVDV
Sbjct: 121 FASCVEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDV 180

Query: 344 VNDRINHCLSHAAGVADECEVCAGTGKCRFN 373
           VNDRI+HCLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 VNDRISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of Spg038463 vs. ExPASy TrEMBL
Match: A0A0A0KP17 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.6e-94
Identity = 176/209 (84.21%), Postives = 189/209 (90.43%), Query Frame = 0 

Query: 164 MRLLSITPQITVKSPSASETRRTFTISASQKSPILIGRPSRFKNFTLRFRMSVDNGSPNR 223
           M   SITPQI+ KS S  ETRR F+I ASQ+S I +GR S+F+NFT RFRMS+DNGS N 
Sbjct: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSN- 60

Query: 224 SRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFA 283
              RIGAGDAVIIVDHGSRR+ESNLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSF 
Sbjct: 61  ---RIGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFT 120

Query: 284 SCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVN 343
            CVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAK+HPGISYIITAPLGLHEQLVDVVN
Sbjct: 121 LCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVN 180

Query: 344 DRINHCLSHAAGVADECEVCAGTGKCRFN 373
           DRIN+CLSHAAGV+DECEVCAGTGKCRFN
Sbjct: 181 DRINYCLSHAAGVSDECEVCAGTGKCRFN 205

[Close]
BLAST of Spg038463 vs. TAIR 10
Match: AT1G50170.1 (sirohydrochlorin ferrochelatase B )

HSP 1 Score: 240.7 bits (613), Expect = 1.8e-63
Identity = 110/146 (75.34%), Postives = 126/146 (86.30%), Query Frame = 0 

Query: 225 RDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFAS 284
           ++ IG  D +IIVDHGSRR+ESNLML EFV MF++KTGYPIVEPAHMELAEPSI+D+F+ 
Sbjct: 75  KNGIGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSL 134

Query: 285 CVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKEHPGISYIITAPLGLHEQLVDVVND 344
           CV+QGAKR++VSPFFLFPGRHW  DIPSLTA+AAKE  GISY+ITAPLG H  L+DVVND
Sbjct: 135 CVQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVND 194

Query: 345 RINHCLSHAAGVADECEVCAGTGKCR 371
           RI HCLSH  G ADEC VCAGT KC+
Sbjct: 195 RIQHCLSHVEGDADECLVCAGTNKCK 220

The following BLAST results are available for this feature:
BLAST of Spg038463 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038890885.13.5e-10184.72sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida][more]
KAG7033101.15.7e-9988.10Sirohydrochlorin ferrochelatase, chloroplastic, partial [Cucurbita argyrosperma ... [more]
XP_023548507.12.4e-9786.67sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022993758.12.0e-9686.26sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita maxima][more]
KAA0037556.12.0e-9686.12sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydro... [more]
BLAST of Spg038463 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q84JH72.6e-6275.34Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 G... [more]
P618176.2e-1635.34Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1[more]
P618192.2e-1329.08Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) ... [more]
Q583801.4e-1232.61Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC ... [more]
A6UWT52.7e-1128.99Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-128... [more]
BLAST of Spg038463 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3BY559.7e-9786.12Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1K3849.7e-9786.26sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 ... [more]
A0A1S3C8S99.7e-9786.12sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1FHT51.1e-9585.78sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=366... [more]
A0A0A0KP171.6e-9484.21Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1[more]
BLAST of Spg038463 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G50170.11.8e-6375.34sirohydrochlorin ferrochelatase B [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.1400coord: 230..353
e-value: 1.9E-34
score: 120.5
NoneNo IPR availablePANTHERPTHR33542:SF3SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 222..369
NoneNo IPR availablePANTHERPTHR33542SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 222..369
NoneNo IPR availableCDDcd03416CbiX_SirB_Ncoord: 233..333
e-value: 3.49971E-37
score: 128.073
NoneNo IPR availableSUPERFAMILY53800Chelatasecoord: 232..349
IPR002762Sirohydrochlorin cobaltochelatase CbiX-likePFAMPF01903CbiXcoord: 239..343
e-value: 7.3E-30
score: 103.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg038463.1Spg038463.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006979 response to oxidative stress
biological_process GO:0019354 siroheme biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0042802 identical protein binding
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051266 sirohydrochlorin ferrochelatase activity
molecular_function GO:0016829 lyase activity