Spg038430 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg038430
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	scaffold12: 5164704 .. 5165759 (-)
RNA-Seq Expression	Spg038430
Synteny	Spg038430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGAGAGATTTTCAACTTCGTCAAGGTCTGGTTAGCAGTCTGTATCTCTCTCTGTTACTGCCACGCCGCCGGAAAACTCCTCCCTCCCGGCGCCGGCCGCTTCCTCGCCGTCGCCCCCGTCGTCGCCCTCTTCCTCCTCCTCCCTCTGCAACTCACCTCCATCCACCTCGGCGGCTCCACCTCCTTCTTCGTCGCTTGGCTCGCTAACTTCAAGCTCCTCCTCTTCGCCGCCGGCCACGGCCCTCTCTCCGCCGCCCCGCCGCTCCCTCTCCGCCGCTTCCTCGCACTCTCCTGCTTGCCGATCAAGATCCTCCAAAACCCCTCGGAGAAATCGAGCCCTAACAATCGCCCCGAAACCCTAACCCTAAGACCGAAATCGTTCGGAAATTACGCGATTAAAGCCCTAATCGTAGCGCTGATGGTGAAATCGTATGATTACTCTGAGTTTCTTCACCCGAAACTTGTTCTGTTCATTTATAGTTGGCATGTGTACTTCCTTCTCGAGATTATTTTGGCCATCACGCAGTTCCTCGGTCGGAATCTGCTCGGAATCGAGCTCGAGCCGCAGTTCAACGATCCGTATTTCTCGACTTCGCTTCAGGATTTCTGGGGGAGGCGGTGGAATCTGATGGCAACGAACATTCTTCAGCCGACGGTTTACAAGCCGTCGGTGAAGATCGCGGCTAAGGTCGTCGGGCGGAAGTGGGCGCCATTGCCGGCTGTGATGGCGACGTTTATGGTGTCGGCGATGATGCACGAACTGATATTTTACTATTTGGGTCGGATGAGGCCCAACTGGGAGATTACTTGGTTCTTTGTCATCCACGGGGTTGCTTTGACGGTGGAGATTCTGGTGAAGAAGGCGTTGCCGGAGAAGTGGCGGGTGCCGGCGGTGGTGTCGGTGCCGGTGACTGTGGGTTTCATCTTCTCGACGGCGATTTGGTTGTTTTTTCCGCAGTTCGTGAGGTTGAAGGTGGACGTCAGAGCCTTGCAGGAGTACGCTGCGTTGGTCACTTTTGTCAAGAAACTCGCTTTCTCATTGGTCAGTTGA

mRNA sequence

ATGGAGGGAGAGATTTTCAACTTCGTCAAGGTCTGGTTAGCAGTCTGTATCTCTCTCTGTTACTGCCACGCCGCCGGAAAACTCCTCCCTCCCGGCGCCGGCCGCTTCCTCGCCGTCGCCCCCGTCGTCGCCCTCTTCCTCCTCCTCCCTCTGCAACTCACCTCCATCCACCTCGGCGGCTCCACCTCCTTCTTCGTCGCTTGGCTCGCTAACTTCAAGCTCCTCCTCTTCGCCGCCGGCCACGGCCCTCTCTCCGCCGCCCCGCCGCTCCCTCTCCGCCGCTTCCTCGCACTCTCCTGCTTGCCGATCAAGATCCTCCAAAACCCCTCGGAGAAATCGAGCCCTAACAATCGCCCCGAAACCCTAACCCTAAGACCGAAATCGTTCGGAAATTACGCGATTAAAGCCCTAATCGTAGCGCTGATGGTGAAATCGTATGATTACTCTGAGTTTCTTCACCCGAAACTTGTTCTGTTCATTTATAGTTGGCATGTGTACTTCCTTCTCGAGATTATTTTGGCCATCACGCAGTTCCTCGGTCGGAATCTGCTCGGAATCGAGCTCGAGCCGCAGTTCAACGATCCGTATTTCTCGACTTCGCTTCAGGATTTCTGGGGGAGGCGGTGGAATCTGATGGCAACGAACATTCTTCAGCCGACGGTTTACAAGCCGTCGGTGAAGATCGCGGCTAAGGTCGTCGGGCGGAAGTGGGCGCCATTGCCGGCTGTGATGGCGACGTTTATGGTGTCGGCGATGATGCACGAACTGATATTTTACTATTTGGGTCGGATGAGGCCCAACTGGGAGATTACTTGGTTCTTTGTCATCCACGGGGTTGCTTTGACGGTGGAGATTCTGGTGAAGAAGGCGTTGCCGGAGAAGTGGCGGGTGCCGGCGGTGGTGTCGGTGCCGGTGACTGTGGGTTTCATCTTCTCGACGGCGATTTGGTTGTTTTTTCCGCAGTTCGTGAGGTTGAAGGTGGACGTCAGAGCCTTGCAGGAGTACGCTGCGTTGGTCACTTTTGTCAAGAAACTCGCTTTCTCATTGGTCAGTTGA

Coding sequence (CDS)

ATGGAGGGAGAGATTTTCAACTTCGTCAAGGTCTGGTTAGCAGTCTGTATCTCTCTCTGTTACTGCCACGCCGCCGGAAAACTCCTCCCTCCCGGCGCCGGCCGCTTCCTCGCCGTCGCCCCCGTCGTCGCCCTCTTCCTCCTCCTCCCTCTGCAACTCACCTCCATCCACCTCGGCGGCTCCACCTCCTTCTTCGTCGCTTGGCTCGCTAACTTCAAGCTCCTCCTCTTCGCCGCCGGCCACGGCCCTCTCTCCGCCGCCCCGCCGCTCCCTCTCCGCCGCTTCCTCGCACTCTCCTGCTTGCCGATCAAGATCCTCCAAAACCCCTCGGAGAAATCGAGCCCTAACAATCGCCCCGAAACCCTAACCCTAAGACCGAAATCGTTCGGAAATTACGCGATTAAAGCCCTAATCGTAGCGCTGATGGTGAAATCGTATGATTACTCTGAGTTTCTTCACCCGAAACTTGTTCTGTTCATTTATAGTTGGCATGTGTACTTCCTTCTCGAGATTATTTTGGCCATCACGCAGTTCCTCGGTCGGAATCTGCTCGGAATCGAGCTCGAGCCGCAGTTCAACGATCCGTATTTCTCGACTTCGCTTCAGGATTTCTGGGGGAGGCGGTGGAATCTGATGGCAACGAACATTCTTCAGCCGACGGTTTACAAGCCGTCGGTGAAGATCGCGGCTAAGGTCGTCGGGCGGAAGTGGGCGCCATTGCCGGCTGTGATGGCGACGTTTATGGTGTCGGCGATGATGCACGAACTGATATTTTACTATTTGGGTCGGATGAGGCCCAACTGGGAGATTACTTGGTTCTTTGTCATCCACGGGGTTGCTTTGACGGTGGAGATTCTGGTGAAGAAGGCGTTGCCGGAGAAGTGGCGGGTGCCGGCGGTGGTGTCGGTGCCGGTGACTGTGGGTTTCATCTTCTCGACGGCGATTTGGTTGTTTTTTCCGCAGTTCGTGAGGTTGAAGGTGGACGTCAGAGCCTTGCAGGAGTACGCTGCGTTGGTCACTTTTGTCAAGAAACTCGCTTTCTCATTGGTCAGTTGA

Protein sequence

MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGGSTSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPETLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLGRNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPLPAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPAVVSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLVS

Homology

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BLAST of Spg038430 vs. NCBI nr
Match: XP_022948970.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 608.2 bits (1567), Expect = 4.4e-170
Identity = 302/350 (86.29%), Postives = 321/350 (91.71%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF+KVWL VCISLCYCHA GKLL PG GRFLAVAP+V LF LLPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
           STSFF+AWLANFKLLLFAAG GPLSA PPLPLRRFLALSCLPIKILQN  EKS PNN   
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
             TL+PKSF NY+IKA+IVALMVK YDYSEF+HPK+VLFIYSWH+YFLLEIILAITQF G
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           RN+LGIELEPQF DPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKV+GRKWAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPAV 300
           PAVMATF+VSAMMHELIFYY+GRMRPNWE TWFFVIHGVALTVEI+VKK LPEKWR+P  
Sbjct: 241 PAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLV 351
           VSVPVT+ FIF TAIWLFFPQFVRLK+DVRA +EYAALVTFVK +AFSLV
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLV 348

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BLAST of Spg038430 vs. NCBI nr
Match: XP_022998466.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 607.8 bits (1566), Expect = 5.8e-170
Identity = 300/350 (85.71%), Postives = 321/350 (91.71%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF+KVWL VCISLCYCHA GKL+PPGAGRFLAVAP+V LF  LPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
           STSFF+AWLANFKLLLF AG GPLSA PPLPLRRFLALSCLPIKILQN  EKS PNN   
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
             TL+PKSF NY+IKA+IVALMVK YDYSEF+HPK+VLFIYSWH+YFLLEIILAITQF G
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           RN+LGIELEPQF DPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKV+GRKWAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPAV 300
           PAVMATF+VSAMMHELIF+Y+GRMRPNWE TWFFVIHGVALTVEI+VKK LPEKWR+P  
Sbjct: 241 PAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLV 351
           VSVPVT+ FIF TAIWLFFPQFVRLK+DVRA +EYAALVTFVK +AFSLV
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLV 348

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BLAST of Spg038430 vs. NCBI nr
Match: XP_022934183.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 604.7 bits (1558), Expect = 4.9e-169
Identity = 295/351 (84.05%), Postives = 322/351 (91.74%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGE+ NF+ VWL VC+SLCYCH AGK+LPPGAGRFLA+APVV LF LLPLQLTS+HLGG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
           ST+FF+AWLANFKLLLF AG+GPLSA PPLPLRRFLALSCLPIKILQNPSEK SP N  E
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
           TL   PKSF NY IKALIVALMVK YDYSEF+HPK++LFIYSWHVYF LEIILAITQF G
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           R LLGIELEPQF+DPYFSTSLQDFWGRRWNLMATNILQPT+YKP+VKIAAKVVGRKWAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPAV 300
           P VMATF+VSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEILVKK LPEK R+PA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLVS 352
           VSVPVT+ FIFSTAIWLFFPQF+RLK+DV+A +EYAALV+F+KKLAF LVS
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLVS 351

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BLAST of Spg038430 vs. NCBI nr
Match: XP_023527814.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 604.0 bits (1556), Expect = 8.3e-169
Identity = 296/351 (84.33%), Postives = 322/351 (91.74%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF  VWL VC+SLCYCH AGK+LPPGAGRFLA+APVV LF LLPLQLTS+HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVLLFFLLPLQLTSVHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
           +T+FF+AWLANFKLLLFAAG+GPLSA PPLPLRRFLALSCLPIKILQNPSEK SP N  E
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
           TL   PKSF NY IKALIVALMVK YDYSEF+HPK++LFIYSWHVYF LEIILAITQF G
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           R LLGIELEPQF+DPYFSTSLQDFWGRRWNLMATNILQPT+YKP+VKIAAKVVGRKWAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPAV 300
           P VMATF+VSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEILVKK LPEK R+PA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLVS 352
           VSVPVT+ FIFSTAIWLFFPQF+RLK+DV+A +EYAALV+F+KKLAF LVS
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLVS 351

[Close]

BLAST of Spg038430 vs. NCBI nr
Match: XP_022982638.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 599.0 bits (1543), Expect = 2.7e-167
Identity = 295/351 (84.05%), Postives = 321/351 (91.45%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF  VWL VC+SLCYCH AGK+L PGAGRFLA++PVV LF LLPLQLTS+HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
           +T+FF+AWLANFKLLLFAAG+GPLSA PPLPLRRFLALSCLPIKILQNPSEK SP N  E
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
           TL   PKSF NY IKALIVALMVK YDYSEF+HPK++LFIYSWHVYF LEIILAITQF G
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           R LLGIELEPQF+DPYFSTSLQDFWGRRWNLMATNILQPTVYKP+VKIAAKVVGRKWAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPAV 300
           P VMATF+VSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEILVKK LPEK R+PA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLVS 352
           VSVPVT+ FIFSTAIWLFFPQF+RLK+DV+A +EYAALV+F+KKLAF LVS
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKKLAFPLVS 351

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BLAST of Spg038430 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.5e-85
Identity = 170/346 (49.13%), Postives = 233/346 (67.34%), Query Frame = 0


Query: 7   NFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGGSTSFFV 66
           +F+K W  V ISLCY     KL+P G  R +   PV  +F ++P  + S+HL G T+FF+
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFAAGHGPLSA-APPLPLRRFLALSCLPIKILQNPSEKSSPNNRPETLTLR 126
           AWLANFKLLLFA G GPLS+   PL L  FLA+SCLPIKI  +P  K +  +  E  T  
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSP--KPTKTHSHEGSTEG 122

Query: 127 PKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLGRNLLG 186
           P     Y IKA+ V L++K+Y+YS  L  K+VL +Y+ H+YF LEIILA T    R +  
Sbjct: 123 PLI---YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSD 182

Query: 187 IELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPLPAVMA 246
           +ELEPQFN PY +TSLQDFWGRRWNLM T IL+PTVY+PS+++ + V+G  ++ + A   
Sbjct: 183 LELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFG 242

Query: 247 TFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPAVVSVPV 306
           TF+VS +MHELIF+Y+GR+RP+W++ WFF+I+G   TVEI +KK +  +WR P  +S  +
Sbjct: 243 TFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVL 302

Query: 307 TVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLVS 352
           T+ F+  TA+WLF P+F R  +  +AL EYAA+  F  ++   L +
Sbjct: 303 TLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKLTA 342

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BLAST of Spg038430 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 6.8e-73
Identity = 153/346 (44.22%), Postives = 214/346 (61.85%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           M+ E+ N +KVW++  IS+ YC+     +  GA RFL+V+PV+ALFL+LPL  +S+HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
            T+FF+ WLANFKL+LF+   GPL    P    RF   +C PIK+ QNP    S N+ P+
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPI-PTNFPRFFCFTCFPIKVQQNP---KSQNHLPK 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
            +         +AIK  I A+++  Y Y + L P ++L +Y  H+Y  +EIIL   + + 
Sbjct: 121 LV---------FAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVV 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
              LG +LEPQ N PY +TSLQDFWGRRWNLM   IL+P VY P  +++ + +   WA  
Sbjct: 181 FISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKK--ALPEKWRVP 300
           P ++A F+VS ++HEL+F+YL R  P  E+T FFV+HGV   VE+ VKK   + ++WR+ 
Sbjct: 241 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 300

Query: 301 AVVSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKK 345
             VS  +TVGF+F T  WLF PQ  R  V  R   E   LV F+K+
Sbjct: 301 PGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of Spg038430 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.6e-72
Identity = 150/351 (42.74%), Postives = 216/351 (61.54%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLP-PGAGRFLAVAPVVALFLLLPLQLTSIHLG 60
           +E E+  F +VW++   + CYC     + P  GA R L + PVV LF+ LPL+L+S HLG
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLG 62

Query: 61  GSTSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRP 120
           G T+ ++ WLANFKLLLFA   GPLS  P L L  F++ + LPIK   +PS     N R 
Sbjct: 63  GPTALYLVWLANFKLLLFAFHLGPLS-NPSLSLLHFISTTLLPIKFRDDPSNDHEKNKR- 122

Query: 121 ETLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFL 180
            TL+   +    +  K +  A ++K Y++ + L   ++  +Y +H Y   EI LA +  +
Sbjct: 123 -TLSFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVI 182

Query: 181 GRNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAP 240
            R  LG++L PQFN+PY +TSLQDFWGRRWNLM ++IL  T Y+P  ++ ++ V  +W  
Sbjct: 183 ARATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEV 242

Query: 241 LPAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPA 300
             A++  F VS +MHE+ F+YL R RP+WE+T FFV+HGV   VE++VKKA+  K R+  
Sbjct: 243 AGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRR 302

Query: 301 VVSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLV 351
            VS  +TVGF+  T  WLF PQ VR  VD++ + EY  +  + +K    L+
Sbjct: 303 EVSGALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLMGLL 350

[Close]

BLAST of Spg038430 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.7e-68
Identity = 148/350 (42.29%), Postives = 205/350 (58.57%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           ME E+ + +KVW+   IS+ YC+     +  G  R L+V PV  LFL+LPL ++S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
           ST+FF++WLANFKL+LF+   GPL   P   L RF+  +C PIK+ QNP     P N+  
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNP----KPQNQ-- 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
                PK    +A+K     +++  Y+Y + + P ++L +YS H+Y   EI+LA  + L 
Sbjct: 121 ----MPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLL 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
              L  +LEP FN+PY STSLQDFWGRRWNLM   IL+P VY P  ++A + +    A  
Sbjct: 181 SISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALF 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKA-LPEKWRVPA 300
             V A+F+VS ++HELIF+Y  R  P  E+T FFV+HGV    E   K+  L  +W+V  
Sbjct: 241 LGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQ 300

Query: 301 VVSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSL 350
           +VS  +TVGF+  T  WLFFP   R  +  R   E    + FVK   F L
Sbjct: 301 MVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFFYL 337

[Close]

BLAST of Spg038430 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 3.0e-68
Identity = 147/347 (42.36%), Postives = 210/347 (60.52%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           ME E+ NF+K+W++  IS+ YC+     +  G  R L+V PV ALFL+ PL  + +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLP--LRRFLALSCLPIKILQNPSEKSSPNNR 120
             +FF++WLANFKL+LF+   GPLS   PLP  L RF+ ++C PIK  QNP+ +    N 
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQNPNIQ----NY 120

Query: 121 PETLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQF 180
              + L       +AIK +I  ++++ Y+Y ++L P L+L   S H++  LEI+  + + 
Sbjct: 121 KIPIWL-------FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKA 180

Query: 181 LGRNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWA 240
           L    LG +LEPQ N+PY +TSLQDFWGRRWNLM   IL+P VY P+ ++A + V    A
Sbjct: 181 LVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQA 240

Query: 241 PLPAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKA--LPEKWR 300
               V A F+VS  +HE++F+YL R  P  E+TWFF++HGV    E+ VKK+  +   WR
Sbjct: 241 MFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWR 300

Query: 301 VPAVVSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVK 344
           V   VS  +TVGF+  T+ W FFP  +R  +  R   E    + FVK
Sbjct: 301 VSPTVSRLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVK 332

[Close]

BLAST of Spg038430 vs. ExPASy TrEMBL
Match: A0A6J1GBE5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452458 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.1e-170
Identity = 302/350 (86.29%), Postives = 321/350 (91.71%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF+KVWL VCISLCYCHA GKLL PG GRFLAVAP+V LF LLPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
           STSFF+AWLANFKLLLFAAG GPLSA PPLPLRRFLALSCLPIKILQN  EKS PNN   
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
             TL+PKSF NY+IKA+IVALMVK YDYSEF+HPK+VLFIYSWH+YFLLEIILAITQF G
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           RN+LGIELEPQF DPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKV+GRKWAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPAV 300
           PAVMATF+VSAMMHELIFYY+GRMRPNWE TWFFVIHGVALTVEI+VKK LPEKWR+P  
Sbjct: 241 PAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLV 351
           VSVPVT+ FIF TAIWLFFPQFVRLK+DVRA +EYAALVTFVK +AFSLV
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLV 348

[Close]

BLAST of Spg038430 vs. ExPASy TrEMBL
Match: A0A6J1KAA2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493091 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.8e-170
Identity = 300/350 (85.71%), Postives = 321/350 (91.71%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF+KVWL VCISLCYCHA GKL+PPGAGRFLAVAP+V LF  LPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
           STSFF+AWLANFKLLLF AG GPLSA PPLPLRRFLALSCLPIKILQN  EKS PNN   
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
             TL+PKSF NY+IKA+IVALMVK YDYSEF+HPK+VLFIYSWH+YFLLEIILAITQF G
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           RN+LGIELEPQF DPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKV+GRKWAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPAV 300
           PAVMATF+VSAMMHELIF+Y+GRMRPNWE TWFFVIHGVALTVEI+VKK LPEKWR+P  
Sbjct: 241 PAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLV 351
           VSVPVT+ FIF TAIWLFFPQFVRLK+DVRA +EYAALVTFVK +AFSLV
Sbjct: 301 VSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLV 348

[Close]

BLAST of Spg038430 vs. ExPASy TrEMBL
Match: A0A6J1F6Y6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441427 PE=3 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.4e-169
Identity = 295/351 (84.05%), Postives = 322/351 (91.74%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGE+ NF+ VWL VC+SLCYCH AGK+LPPGAGRFLA+APVV LF LLPLQLTS+HLGG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
           ST+FF+AWLANFKLLLF AG+GPLSA PPLPLRRFLALSCLPIKILQNPSEK SP N  E
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
           TL   PKSF NY IKALIVALMVK YDYSEF+HPK++LFIYSWHVYF LEIILAITQF G
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           R LLGIELEPQF+DPYFSTSLQDFWGRRWNLMATNILQPT+YKP+VKIAAKVVGRKWAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPAV 300
           P VMATF+VSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEILVKK LPEK R+PA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLVS 352
           VSVPVT+ FIFSTAIWLFFPQF+RLK+DV+A +EYAALV+F+KKLAF LVS
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLVS 351

[Close]

BLAST of Spg038430 vs. ExPASy TrEMBL
Match: A0A6J1J5C4 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481453 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.3e-167
Identity = 295/351 (84.05%), Postives = 321/351 (91.45%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           MEGEI NF  VWL VC+SLCYCH AGK+L PGAGRFLA++PVV LF LLPLQLTS+HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
           +T+FF+AWLANFKLLLFAAG+GPLSA PPLPLRRFLALSCLPIKILQNPSEK SP N  E
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
           TL   PKSF NY IKALIVALMVK YDYSEF+HPK++LFIYSWHVYF LEIILAITQF G
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
           R LLGIELEPQF+DPYFSTSLQDFWGRRWNLMATNILQPTVYKP+VKIAAKVVGRKWAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPAV 300
           P VMATF+VSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEILVKK LPEK R+PA 
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLVS 352
           VSVPVT+ FIFSTAIWLFFPQF+RLK+DV+A +EYAALV+F+KKLAF LVS
Sbjct: 301 VSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKKLAFPLVS 351

[Close]

BLAST of Spg038430 vs. ExPASy TrEMBL
Match: A0A0A0L9S2 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 3.0e-156
Identity = 281/351 (80.06%), Postives = 305/351 (86.89%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           ME EI N  KVWL V ISL YCHA  KLL PG  RFLA+APVV +F LLPLQLTSIH GG
Sbjct: 28  MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 87

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSP-NNRP 120
           +T FFV WLA FKLLL  AG GPLS  PPLPL RF+A+SCLPIKIL+NPSEKS+P NNR 
Sbjct: 88  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 147

Query: 121 ETLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFL 180
           ETL  +PKSF NY+I+ALIV LMVK YDYS F HPKLVLFIYSWHVY LLEIILAITQF 
Sbjct: 148 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 207

Query: 181 GRNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAP 240
           GRNLLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP VYKP+VKIAA+V+GR WAP
Sbjct: 208 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 267

Query: 241 LPAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPA 300
           LP VMATF+VSAMMHELIFYYLGRMRPNWEITWFFVIHG+ALTVEI++KK LPEK R+PA
Sbjct: 268 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 327

Query: 301 VVSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLV 351
            VSVPVT+ FIFSTAIWLFFPQFVRL++DVRA +EYAAL  FVKK+AFSLV
Sbjct: 328 KVSVPVTLVFIFSTAIWLFFPQFVRLRLDVRAFEEYAALANFVKKVAFSLV 378

[Close]

BLAST of Spg038430 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 316.6 bits (810), Expect = 2.5e-86
Identity = 170/346 (49.13%), Postives = 233/346 (67.34%), Query Frame = 0


Query: 7   NFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGGSTSFFV 66
           +F+K W  V ISLCY     KL+P G  R +   PV  +F ++P  + S+HL G T+FF+
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFAAGHGPLSA-APPLPLRRFLALSCLPIKILQNPSEKSSPNNRPETLTLR 126
           AWLANFKLLLFA G GPLS+   PL L  FLA+SCLPIKI  +P  K +  +  E  T  
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSP--KPTKTHSHEGSTEG 122

Query: 127 PKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLGRNLLG 186
           P     Y IKA+ V L++K+Y+YS  L  K+VL +Y+ H+YF LEIILA T    R +  
Sbjct: 123 PLI---YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSD 182

Query: 187 IELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPLPAVMA 246
           +ELEPQFN PY +TSLQDFWGRRWNLM T IL+PTVY+PS+++ + V+G  ++ + A   
Sbjct: 183 LELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFG 242

Query: 247 TFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVPAVVSVPV 306
           TF+VS +MHELIF+Y+GR+RP+W++ WFF+I+G   TVEI +KK +  +WR P  +S  +
Sbjct: 243 TFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVL 302

Query: 307 TVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSLVS 352
           T+ F+  TA+WLF P+F R  +  +AL EYAA+  F  ++   L +
Sbjct: 303 TLTFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKLTA 342

[Close]

BLAST of Spg038430 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 275.8 bits (704), Expect = 4.8e-74
Identity = 153/346 (44.22%), Postives = 214/346 (61.85%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           M+ E+ N +KVW++  IS+ YC+     +  GA RFL+V+PV+ALFL+LPL  +S+HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
            T+FF+ WLANFKL+LF+   GPL    P    RF   +C PIK+ QNP    S N+ P+
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPI-PTNFPRFFCFTCFPIKVQQNP---KSQNHLPK 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
            +         +AIK  I A+++  Y Y + L P ++L +Y  H+Y  +EIIL   + + 
Sbjct: 121 LV---------FAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVV 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
              LG +LEPQ N PY +TSLQDFWGRRWNLM   IL+P VY P  +++ + +   WA  
Sbjct: 181 FISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKK--ALPEKWRVP 300
           P ++A F+VS ++HEL+F+YL R  P  E+T FFV+HGV   VE+ VKK   + ++WR+ 
Sbjct: 241 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 300

Query: 301 AVVSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKK 345
             VS  +TVGF+F T  WLF PQ  R  V  R   E   LV F+K+
Sbjct: 301 PGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of Spg038430 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.2e-69
Identity = 148/350 (42.29%), Postives = 205/350 (58.57%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           ME E+ + +KVW+   IS+ YC+     +  G  R L+V PV  LFL+LPL ++S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
           ST+FF++WLANFKL+LF+   GPL   P   L RF+  +C PIK+ QNP     P N+  
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNP----KPQNQ-- 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
                PK    +A+K     +++  Y+Y + + P ++L +YS H+Y   EI+LA  + L 
Sbjct: 121 ----MPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLL 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
              L  +LEP FN+PY STSLQDFWGRRWNLM   IL+P VY P  ++A + +    A  
Sbjct: 181 SISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALF 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKA-LPEKWRVPA 300
             V A+F+VS ++HELIF+Y  R  P  E+T FFV+HGV    E   K+  L  +W+V  
Sbjct: 241 LGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQ 300

Query: 301 VVSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVKKLAFSL 350
           +VS  +TVGF+  T  WLFFP   R  +  R   E    + FVK   F L
Sbjct: 301 MVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFFYL 337

[Close]

BLAST of Spg038430 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 260.4 bits (664), Expect = 2.1e-69
Identity = 147/347 (42.36%), Postives = 210/347 (60.52%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           ME E+ NF+K+W++  IS+ YC+     +  G  R L+V PV ALFL+ PL  + +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLP--LRRFLALSCLPIKILQNPSEKSSPNNR 120
             +FF++WLANFKL+LF+   GPLS   PLP  L RF+ ++C PIK  QNP+ +    N 
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQNPNIQ----NY 120

Query: 121 PETLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQF 180
              + L       +AIK +I  ++++ Y+Y ++L P L+L   S H++  LEI+  + + 
Sbjct: 121 KIPIWL-------FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKA 180

Query: 181 LGRNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWA 240
           L    LG +LEPQ N+PY +TSLQDFWGRRWNLM   IL+P VY P+ ++A + V    A
Sbjct: 181 LVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQA 240

Query: 241 PLPAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKA--LPEKWR 300
               V A F+VS  +HE++F+YL R  P  E+TWFF++HGV    E+ VKK+  +   WR
Sbjct: 241 MFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWR 300

Query: 301 VPAVVSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVK 344
           V   VS  +TVGF+  T+ W FFP  +R  +  R   E    + FVK
Sbjct: 301 VSPTVSRLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVK 332

[Close]

BLAST of Spg038430 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 249.2 bits (635), Expect = 4.9e-66
Identity = 139/345 (40.29%), Postives = 202/345 (58.55%), Query Frame = 0


Query: 1   MEGEIFNFVKVWLAVCISLCYCHAAGKLLPPGAGRFLAVAPVVALFLLLPLQLTSIHLGG 60
           ME +  N ++VW++  ISL YC+     L  G  R L++ PV  LFL+LPL L+ +H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  STSFFVAWLANFKLLLFAAGHGPLSAAPPLPLRRFLALSCLPIKILQNPSEKSSPNNRPE 120
            +  F++WLANFKLLLFA   GPL   PP  L RF+  +CLPIKI Q+PS  + PN  P 
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIPNLHP- 120

Query: 121 TLTLRPKSFGNYAIKALIVALMVKSYDYSEFLHPKLVLFIYSWHVYFLLEIILAITQFLG 180
               +P      A+K L++ +++  Y+Y + L   +VL +Y  H+Y  +E++L     + 
Sbjct: 121 ----KPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVV 180

Query: 181 RNLLGIELEPQFNDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVVGRKWAPL 240
             LLG  +EP FN+PY +TSLQDFW RRWNLM + +L+ TV+ P  +   +++    A  
Sbjct: 181 STLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMF 240

Query: 241 PAVMATFMVSAMMHELIFYYLGRMRPNWEITWFFVIHGVALTVEILVKKALPEKWRVP-- 300
             VMA+F VS +MHEL+++Y+ R  P WE+T FFV+HG A   EI VK+    +W  P  
Sbjct: 241 AGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT---QWLRPPH 300

Query: 301 AVVSVPVTVGFIFSTAIWLFFPQFVRLKVDVRALQEYAALVTFVK 344
             VS  V + F+  T +WLF  Q +R  V  +A+ E   ++   K
Sbjct: 301 RAVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAK 336

The following BLAST results are available for this feature:

BLAST of Spg038430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948970.1	4.4e-170	86.29	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acy...	[more]
XP_022998466.1	5.8e-170	85.71	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
XP_022934183.1	4.9e-169	84.05	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata]	[more]
XP_023527814.1	8.3e-169	84.33	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022982638.1	2.7e-167	84.05	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]

BLAST of Spg038430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	3.5e-85	49.13	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9FJ76	6.8e-73	44.22	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9XGY6	2.6e-72	42.74	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ75	1.7e-68	42.29	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ74	3.0e-68	42.36	Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX...	[more]

BLAST of Spg038430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GBE5	2.1e-170	86.29	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KAA2	2.8e-170	85.71	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F6Y6	2.4e-169	84.05	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1J5C4	1.3e-167	84.05	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A0A0L9S2	3.0e-156	80.06	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=...	[more]

BLAST of Spg038430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	2.5e-86	49.13	acyl-CoA sterol acyl transferase 1	[more]
AT5G55340.1	4.8e-74	44.22	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	1.2e-69	42.29	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	2.1e-69	42.36	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	4.9e-66	40.29	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 189..275 e-value: 1.1E-15 score: 57.7
IPR017088	Wax synthase, Magnoliopsida	PIRSF	PIRSF037006	Wax_synthase	coord: 1..351 e-value: 1.3E-121 score: 403.9
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 2..346
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 2..346

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg038430.1	Spg038430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity