Spg038419 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg038419
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionP-loop containing nucleoside triphosphate hydrolases superfamily protein
Locationscaffold12: 5771497 .. 5774779 (+)
RNA-Seq ExpressionSpg038419
SyntenySpg038419
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTGGATAAAGAGAAAGTCGCCGAAGAATCTGTTACCAGTCGCTTGTTCAAAATTATTCTCAGTTGGGACTATTTTCGGCTACTCAAGGATTCCAAGGTTGGATTACTTGTACTCAATTCTCCGTTGTCTATTCATGTTTCTCGAATTTAGCTTCGTTTTGTTTACTTTCGGTCTTCTTGAAAGCAGAAACGCAAGGAGGATGATGGGGATGGAGCGGCATTGGGTTTAAAGGAAGTGAAGTCTACGTATAAGGATGTTGATGATTATATCTCTACTTTTGAGCCGCTTTTGTTGGAAGAAGTCAAGGCTCAGATTATTCAGAGGAATGACGATGAAGAAGGTACCATTTTTTTTTTTTGTTCCCTTCTCTTTCTTTGATGCATTCGAGTCGGTCGGGAAACTGTTGAAGAGGGAATTGCAAAAAGAAAAATCATGTTGTGACATGTTCTGTATGTCTTAATGGTAGCTTTGAGAGGGAAGAAACGTTTCTAGATTAGTTGGGTTTGGGGTTTTATGATGGGGTAATAATCTTCTGCCTTATGACATTTCTTAAATTCTAGTACTTGGGATTTGCAGCGGCGGATTGGAAATTCAGGGCGATTATGGAGTGCAATGAAGTCAATGGATTTCACTTTCCAGAGATAGTGTATCTCAGAGATGAGGACCTAAAAGATGAAGACAGCGAAAAGGGTGAATTCCTATCACCAAATGATCTTATGCTTCTCTCCAAGGAGAAGGTGAAGGGAATTTTTTTTTTTCTGCTCTCGTTTTTTTTTGTCCTCTCTTTCTTTATCCCCCATCATACAGAAATTTAGTGAACAGAAGAGAAAAATGCAGGGCTGGCTGGGCAACTTTGTAGTCGTTTTCATTCCCCAGATATCTCCTTATGGAACATTTTTCTTCAAACTCTGTTGGTTTACTTCCCTTTCTTCTTTCAGGTTTGATAGAGAAAATTGAGTCTAATCCACTTAGAGTTTGAAAAAAAAAAAAACTGGAAACCTAAATGAGAGTGGTTAAACGACGAGGAAGAAGTTGGTTCAGATTTGCATGGTTATCTCTTGCAGAGGATATTTAAGATCTAATTCTTTTCTGAATCTTCCCTGAATGTTTGGAGTTGTATGGCTAACATCCTAGTATTCCTTGCAGTTTCAGGAGAATGCAAAACTACCCACCACATATGCCTTTGCATTAGTAGAAAGTCGCCAACAAAGTAAACTCAGGCTTAGAATGTATTTGGCCGGAGAAGTCACACATAAAGGTGTAGAGGCTATTGTATCTTCTCCAAGGCTTCTGAAAGTACGATCTCACGTTACTTCTTCGAGTAAGGATGGAATATATATTTACAGTTTAAAGGTAACAGTTTCCCCTACTAGCTACTTTCTAAAAGTTCTTCTGTGCGGTGGCCTTTTTCCTTCTCATTGCTAGTTTTCTGTTGTTGATGGTACCTGATGGTTCTAAATGATTATGGCAACTATGATGGTTGATGGAACTTGTATAGTTTTTCTGCATGGTGCATCATTTTTTTAGGGGATTTAAGTAAAACCTTCTTCTTGAATCCTTATTTGAGAATATGACTTTTCCTAATTGTGTAAACCTTTTCCCCCCTTGTGTTTGTCACCACACACTTTATACTTAGATGTGTCAATTGGAAGAGCTTCTTGTAATTTCCTTTGTTTCAAAATTGGGCCACCCACCATATCAATGAAATTTGTTTCTTATGATTATTTTTCAAGCCTAAATTAATCTTGTCTAACCATATACTGTAATATATATTGCCTTTTCTTTAAGAAAATAGTTTATGAAAAGCATTTCTGTGATTGGTTTAGTGCCAAAAAGTATCTAGTTGTTTCATCAGTCTTTAGTTGTTGGTTTGTTTAATTTTTAAATTTATTAGTTATGTAGATACTAACAACGAAATAGTTCTCTTATTTGTGGTTAGCTTTTCTAAGAAAATTTGTTGATTCTGAAGATTTGCAGTTTATCGACTATCATTCGTGAATATATAGCATTGTGGTCTATTAGCTCTCTACCTTTTAGGGATATGATATTAGCTGCTGCTGATAAGAATACCGGTAAAGATCAAACCTGGAAAATTTCCAGACCTTTGCAGGATTACATGAAAGAAAATCTTAATGAATCCCAACAGGCAGCTGTACAGGTCAGTAAGTAGCAGTTTGATGAAGTAGAGTAGTAGTTCTTTGTCTGGTATTTATTTTTCCCTACTGTTGATGACTGGTTTAAAGTCTTCAAGATTCATGATTCAATTCAAGTACCTTCCCCTCACTCCCTCTTTTGTCTCTCTTCTCCTGCTCTCTGATTTTTCAATCATTTCACAACTTCATTTATGTCATCTATTTTCTGAACATATATAATTCTTGATTGCAGGCTGGTCTGTCACGTAAACCCTTTGTTCTCATACAGGTCCATCTTATTACGCTTTGTGCTTCTTTTATCTATAATTTTACCTACTTAGAAATGCTTTAACCACTTGACTTGTTTAACTTTGTATCAGTTTATTTATTTTATGGAGATCCATTATTAATTGAGATTGCTCTTGTTGCCTCAGAATTCACTTTTACCAACTATATAAGAACCAAAGAAATAAAAGGAAATAAAATTTATTAGATGCTTGTTGGATTGGGGGTGCTTTTAATCGGTATGATTTTGGAGTGAATTCATTTCTTATTTATATATACTTCTTTTAATAAATTTTTCTTAGTGGTAGATGTGTTTTTTTTCGAGCAAGCCTATTGAGTTCTTCGCCTCTTGTTCTCTCTCTATTGGACTTTTTGTTCAATCCTTTCACATACGTGCCTCTTTCTGTAATTGAATAATGCTTTATTTCTTAATGAAATTGTTTCTTAACAAAAAAAAAAAAAAAAAAAAAAGAAGGTTTGTTTCTATTTCTTCACGTAAAGAGGAACTGAAGCACAAATTTCTGGAACCTTTAATTCCACAAATAAGCAACTAAAGGCATAGATGTTTGGTATCAATCAACTTATGTTCTTTTTCTTCGACTATCCATATCAAAGTTCTTCAAACAAGGTCTCTTTTTAGTCTTTTAATCAGTTGAAATCTGACTCTTTGTTAGATTGTATATTTGAGAAATATGTACAAATTGCAGAGTATTGTCCTGAAAATATTACTATTTTGAAAGTACGAATTCTGACTGTGTTATTCCTTCTAGGGTCCTCCAGGAACAGGGAAGACACAGACTATCCTTGGGCTTCTTAGTGCCATTCTGCATGCCACACCAGCAAGGATGCACTCCAAGTAA

mRNA sequence

ATGGCTGTGGATAAAGAGAAAGTCGCCGAAGAATCTGTTACCAGTCGCTTGTTCAAAATTATTCTCAGTTGGGACTATTTTCGGCTACTCAAGGATTCCAAGAAACGCAAGGAGGATGATGGGGATGGAGCGGCATTGGGTTTAAAGGAAGTGAAGTCTACGTATAAGGATGTTGATGATTATATCTCTACTTTTGAGCCGCTTTTGTTGGAAGAAGTCAAGGCTCAGATTATTCAGAGGAATGACGATGAAGAAGCGGCGGATTGGAAATTCAGGGCGATTATGGAGTGCAATGAAGTCAATGGATTTCACTTTCCAGAGATAGTGTATCTCAGAGATGAGGACCTAAAAGATGAAGACAGCGAAAAGGGTGAATTCCTATCACCAAATGATCTTATGCTTCTCTCCAAGGAGAAGTATTCCTTGCAGTTTCAGGAGAATGCAAAACTACCCACCACATATGCCTTTGCATTAGTAGAAAGTCGCCAACAAAGTAAACTCAGGCTTAGAATGTATTTGGCCGGAGAAGTCACACATAAAGGTGTAGAGGCTATTGTATCTTCTCCAAGGCTTCTGAAAGTACGATCTCACGTTACTTCTTCGAGTAAGGATGGAATATATATTTACAGTTTAAAGGTAACAGTTTCCCCTACTAGCTACTTTCTAAAAGTTCTTCTTTTATCGACTATCATTCGTGAATATATAGCATTGTGGTCTATTAGCTCTCTACCTTTTAGGGATATGATATTAGCTGCTGCTGATAAGAATACCGGTAAAGATCAAACCTGGAAAATTTCCAGACCTTTGCAGGATTACATGAAAGAAAATCTTAATGAATCCCAACAGGCAGCTGTACAGGCTGGTCTGTCACGTAAACCCTTTGTTCTCATACAGGGTCCTCCAGGAACAGGGAAGACACAGACTATCCTTGGGCTTCTTAGTGCCATTCTGCATGCCACACCAGCAAGGATGCACTCCAAGTAA

Coding sequence (CDS)

ATGGCTGTGGATAAAGAGAAAGTCGCCGAAGAATCTGTTACCAGTCGCTTGTTCAAAATTATTCTCAGTTGGGACTATTTTCGGCTACTCAAGGATTCCAAGAAACGCAAGGAGGATGATGGGGATGGAGCGGCATTGGGTTTAAAGGAAGTGAAGTCTACGTATAAGGATGTTGATGATTATATCTCTACTTTTGAGCCGCTTTTGTTGGAAGAAGTCAAGGCTCAGATTATTCAGAGGAATGACGATGAAGAAGCGGCGGATTGGAAATTCAGGGCGATTATGGAGTGCAATGAAGTCAATGGATTTCACTTTCCAGAGATAGTGTATCTCAGAGATGAGGACCTAAAAGATGAAGACAGCGAAAAGGGTGAATTCCTATCACCAAATGATCTTATGCTTCTCTCCAAGGAGAAGTATTCCTTGCAGTTTCAGGAGAATGCAAAACTACCCACCACATATGCCTTTGCATTAGTAGAAAGTCGCCAACAAAGTAAACTCAGGCTTAGAATGTATTTGGCCGGAGAAGTCACACATAAAGGTGTAGAGGCTATTGTATCTTCTCCAAGGCTTCTGAAAGTACGATCTCACGTTACTTCTTCGAGTAAGGATGGAATATATATTTACAGTTTAAAGGTAACAGTTTCCCCTACTAGCTACTTTCTAAAAGTTCTTCTTTTATCGACTATCATTCGTGAATATATAGCATTGTGGTCTATTAGCTCTCTACCTTTTAGGGATATGATATTAGCTGCTGCTGATAAGAATACCGGTAAAGATCAAACCTGGAAAATTTCCAGACCTTTGCAGGATTACATGAAAGAAAATCTTAATGAATCCCAACAGGCAGCTGTACAGGCTGGTCTGTCACGTAAACCCTTTGTTCTCATACAGGGTCCTCCAGGAACAGGGAAGACACAGACTATCCTTGGGCTTCTTAGTGCCATTCTGCATGCCACACCAGCAAGGATGCACTCCAAGTAA

Protein sequence

MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDDYISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDEDSEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSISSLPFRDMILAAADKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSAILHATPARMHSK
Homology
BLAST of Spg038419 vs. NCBI nr
Match: XP_038881790.1 (probable helicase MAGATAMA 3 isoform X2 [Benincasa hispida] >XP_038881791.1 probable helicase MAGATAMA 3 isoform X2 [Benincasa hispida])

HSP 1 Score: 559.3 bits (1440), Expect = 2.2e-155
Identity = 291/327 (88.99%), Postives = 305/327 (93.27%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDD 60
           MAVDKEKVAEESVTSRLFKIILSWDYFRLLK+SKK KED GDGA+LGLKEVKSTYKDVDD
Sbjct: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKNSKKHKEDVGDGASLGLKEVKSTYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDED 120
           YISTFEPLLLEE+KAQIIQRNDDEEAADWKFRAI+EC+EVNGFHFPEIVYLRDE+L DED
Sbjct: 61  YISTFEPLLLEEIKAQIIQRNDDEEAADWKFRAIVECSEVNGFHFPEIVYLRDEELNDED 120

Query: 121 SEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180
           S KGEFLSPNDL+LLSKEK    FQENA+LPTTYAFALVESRQQSKLRLRMYLAGEVTHK
Sbjct: 121 SGKGEFLSPNDLLLLSKEK----FQENARLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180

Query: 181 GVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSI 240
           GVEAIVSSPRLLKVRSH+TSSSKDGIYIYS           LK+  LSTIIREYIALWSI
Sbjct: 181 GVEAIVSSPRLLKVRSHITSSSKDGIYIYS-----------LKICSLSTIIREYIALWSI 240

Query: 241 SSLPFRDMILAAADKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGP 300
           SSLPFR+MILAAADKNTGKDQ WKISRPLQ+YM+ENLNESQQAAVQAGLSRKPFVLIQGP
Sbjct: 241 SSLPFREMILAAADKNTGKDQAWKISRPLQEYMQENLNESQQAAVQAGLSRKPFVLIQGP 300

Query: 301 PGTGKTQTILGLLSAILHATPARMHSK 328
           PGTGKTQTILGLLSAILHATPARMH+K
Sbjct: 301 PGTGKTQTILGLLSAILHATPARMHTK 312

BLAST of Spg038419 vs. NCBI nr
Match: KAG6581597.1 (putative helicase MAGATAMA 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 557.8 bits (1436), Expect = 6.4e-155
Identity = 288/327 (88.07%), Postives = 304/327 (92.97%), Query Frame = 0

Query: 1    MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDD 60
            MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSK++++DDG  A+LGLKEVKS+YKDVDD
Sbjct: 774  MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKQKRKDDGGAASLGLKEVKSSYKDVDD 833

Query: 61   YISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDED 120
            YISTFEPLLLEE+KAQIIQRNDDE+AADWKFRAIM+C+EVNGFHFPEIVYLRDED+KDED
Sbjct: 834  YISTFEPLLLEEIKAQIIQRNDDEDAADWKFRAIMDCSEVNGFHFPEIVYLRDEDIKDED 893

Query: 121  SEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180
             EK EFLSPNDL+LLSKEK    FQENAKLPTTYAFALVESRQ SKLRLRMYLAGEVTHK
Sbjct: 894  GEKAEFLSPNDLLLLSKEK----FQENAKLPTTYAFALVESRQHSKLRLRMYLAGEVTHK 953

Query: 181  GVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSI 240
            GVEAIVSSPRLLKVRSH+TSSSKDGIYIYS           LK+  LSTIIREYIALWSI
Sbjct: 954  GVEAIVSSPRLLKVRSHITSSSKDGIYIYS-----------LKICSLSTIIREYIALWSI 1013

Query: 241  SSLPFRDMILAAADKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGP 300
            SSLPFRDMILAAADKNTG+DQ WKISRPLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGP
Sbjct: 1014 SSLPFRDMILAAADKNTGEDQAWKISRPLQDYMRENLNESQQAAVQAGLSRKPFVLIQGP 1073

Query: 301  PGTGKTQTILGLLSAILHATPARMHSK 328
            PGTGKTQTILGLLSAILHATPARMHSK
Sbjct: 1074 PGTGKTQTILGLLSAILHATPARMHSK 1085

BLAST of Spg038419 vs. NCBI nr
Match: XP_023528758.1 (probable helicase MAGATAMA 3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 557.8 bits (1436), Expect = 6.4e-155
Identity = 288/327 (88.07%), Postives = 304/327 (92.97%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDD 60
           MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSK++++DDG  A+LGLKEVKS+YKDVDD
Sbjct: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKQKRKDDGGAASLGLKEVKSSYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDED 120
           YISTFEPLLLEE+KAQIIQRNDDE+AADWKFRAIM+C+EVNGFHFPEIVYLRDED+KDED
Sbjct: 61  YISTFEPLLLEEIKAQIIQRNDDEDAADWKFRAIMDCSEVNGFHFPEIVYLRDEDIKDED 120

Query: 121 SEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180
            EK EFLSPNDL+LLSKEK    FQENAKLPTTYAFALVESRQ SKLRLRMYLAGEVTHK
Sbjct: 121 GEKAEFLSPNDLLLLSKEK----FQENAKLPTTYAFALVESRQHSKLRLRMYLAGEVTHK 180

Query: 181 GVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSI 240
           GVEAIVSSPRLLKVRSH+TSSSKDGIYIYS           LK+  LSTIIREYIALWSI
Sbjct: 181 GVEAIVSSPRLLKVRSHITSSSKDGIYIYS-----------LKICSLSTIIREYIALWSI 240

Query: 241 SSLPFRDMILAAADKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGP 300
           SSLPFRDMILAAADKNTG+DQ WKISRPLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGP
Sbjct: 241 SSLPFRDMILAAADKNTGEDQAWKISRPLQDYMRENLNESQQAAVQAGLSRKPFVLIQGP 300

Query: 301 PGTGKTQTILGLLSAILHATPARMHSK 328
           PGTGKTQTILGLLSAILHATPARMHSK
Sbjct: 301 PGTGKTQTILGLLSAILHATPARMHSK 312

BLAST of Spg038419 vs. NCBI nr
Match: KAG7018098.1 (putative helicase MAGATAMA 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 557.8 bits (1436), Expect = 6.4e-155
Identity = 288/327 (88.07%), Postives = 304/327 (92.97%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDD 60
           MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSK++++DDG  A+LGLKEVKS+YKDVDD
Sbjct: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKQKRKDDGGAASLGLKEVKSSYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDED 120
           YISTFEPLLLEE+KAQIIQRNDDE+AADWKFRAIM+C+EVNGFHFPEIVYLRDED+KDED
Sbjct: 61  YISTFEPLLLEEIKAQIIQRNDDEDAADWKFRAIMDCSEVNGFHFPEIVYLRDEDIKDED 120

Query: 121 SEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180
            EK EFLSPNDL+LLSKEK    FQENAKLPTTYAFALVESRQ SKLRLRMYLAGEVTHK
Sbjct: 121 GEKAEFLSPNDLLLLSKEK----FQENAKLPTTYAFALVESRQHSKLRLRMYLAGEVTHK 180

Query: 181 GVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSI 240
           GVEAIVSSPRLLKVRSH+TSSSKDGIYIYS           LK+  LSTIIREYIALWSI
Sbjct: 181 GVEAIVSSPRLLKVRSHITSSSKDGIYIYS-----------LKICSLSTIIREYIALWSI 240

Query: 241 SSLPFRDMILAAADKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGP 300
           SSLPFRDMILAAADKNTG+DQ WKISRPLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGP
Sbjct: 241 SSLPFRDMILAAADKNTGEDQAWKISRPLQDYMRENLNESQQAAVQAGLSRKPFVLIQGP 300

Query: 301 PGTGKTQTILGLLSAILHATPARMHSK 328
           PGTGKTQTILGLLSAILHATPARMHSK
Sbjct: 301 PGTGKTQTILGLLSAILHATPARMHSK 312

BLAST of Spg038419 vs. NCBI nr
Match: XP_022935506.1 (probable helicase MAGATAMA 3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 557.8 bits (1436), Expect = 6.4e-155
Identity = 288/327 (88.07%), Postives = 304/327 (92.97%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDD 60
           MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSK++++DDG  A+LGLKEVKS+YKDVDD
Sbjct: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKQKRKDDGGAASLGLKEVKSSYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDED 120
           YISTFEPLLLEE+KAQIIQRNDDE+AADWKFRAIM+C+EVNGFHFPEIVYLRDED+KDED
Sbjct: 61  YISTFEPLLLEEIKAQIIQRNDDEDAADWKFRAIMDCSEVNGFHFPEIVYLRDEDIKDED 120

Query: 121 SEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180
            EK EFLSPNDL+LLSKEK    FQENAKLPTTYAFALVESRQ SKLRLRMYLAGEVTHK
Sbjct: 121 GEKAEFLSPNDLLLLSKEK----FQENAKLPTTYAFALVESRQHSKLRLRMYLAGEVTHK 180

Query: 181 GVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSI 240
           GVEAIVSSPRLLKVRSH+TSSSKDGIYIYS           LK+  LSTIIREYIALWSI
Sbjct: 181 GVEAIVSSPRLLKVRSHITSSSKDGIYIYS-----------LKICSLSTIIREYIALWSI 240

Query: 241 SSLPFRDMILAAADKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGP 300
           SSLPFRDMILAAADKNTG+DQ WKISRPLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGP
Sbjct: 241 SSLPFRDMILAAADKNTGEDQAWKISRPLQDYMRENLNESQQAAVQAGLSRKPFVLIQGP 300

Query: 301 PGTGKTQTILGLLSAILHATPARMHSK 328
           PGTGKTQTILGLLSAILHATPARMHSK
Sbjct: 301 PGTGKTQTILGLLSAILHATPARMHSK 312

BLAST of Spg038419 vs. ExPASy Swiss-Prot
Match: B6SFA4 (Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.0e-75
Identity = 163/330 (49.39%), Postives = 213/330 (64.55%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTS--RLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDV 60
           MA+D  K+ EE  +S  R + IIL WDY +L K+++++   D       L  VK+TYKDV
Sbjct: 1   MAIDNGKLQEEEASSVTRFYNIILGWDYKQLTKENERKNRKDSKEK---LNVVKNTYKDV 60

Query: 61  DDYISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKD 120
           DDY  TFEPLL EEVKAQI+Q  D EEA+  K R +MECNE  GFHF  + Y  +ED   
Sbjct: 61  DDYFETFEPLLFEEVKAQILQNKDGEEASVCKMRLVMECNEGEGFHFLLVTYEHEED--- 120

Query: 121 EDSEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVT 180
                 E+L+ NDL+LLSKE+      +    P++Y FA+VE RQ + LRLRMYLA ++ 
Sbjct: 121 ------EYLAQNDLLLLSKEEV-----KGNSFPSSYGFAVVEHRQNNLLRLRMYLAEDIV 180

Query: 181 HKGVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALW 240
                 I  + +  + +S + + S     I S    +    + LK+  LSTIIREYIAL 
Sbjct: 181 Q-----ITKNTKSSRTKSFIQALSNMRSLITSSASPIDKRVFSLKLCGLSTIIREYIALR 240

Query: 241 SISSLPFRDMILAAADKNTG-KDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLI 300
           S+SSLPF+D+I  AA+K+ G  D+ WKIS PL ++  ENLN+SQ+ A+  GLSRK FVLI
Sbjct: 241 SVSSLPFKDLIFTAAEKSCGFGDEAWKISGPLNEFFNENLNKSQKEAIDVGLSRKSFVLI 300

Query: 301 QGPPGTGKTQTILGLLSAILHATPARMHSK 328
           QGPPGTGKTQTIL +L AI+HATPAR+ SK
Sbjct: 301 QGPPGTGKTQTILSILGAIMHATPARVQSK 308

BLAST of Spg038419 vs. ExPASy Swiss-Prot
Match: Q86AS0 (Probable helicase DDB_G0274399 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274399 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.9e-17
Identity = 97/362 (26.80%), Postives = 147/362 (40.61%), Query Frame = 0

Query: 11  ESVTSRLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDDYISTFEPLLL 70
           + +  R +K IL+WD   L   S K KE         LK VK ++ + +DYI+T+EPLL 
Sbjct: 38  DQIIDRFYKHILTWDASDL---SPKEKE---------LKPVKVSFNNEEDYITTYEPLLF 97

Query: 71  EEVKAQI---IQRNDDEEAADWKFRAIMECNEVNGF------------------------ 130
           EE +AQ+   I+  + ++ ++     +   +EVN F                        
Sbjct: 98  EECRAQLERSIEEGEKDDTSEPTLSRVRYISEVNDFLVVGLVMAENVNIFQFHDNDLIMI 157

Query: 131 --HFPEIVYLRDED---LKDEDSEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFAL 190
             H P IV+  DED     DED+      +       S    +          TT    +
Sbjct: 158 SLHHPLIVFGMDEDEEMTDDEDTAPTSAATHVGAPTKSTTTTTTTTTTTTTTTTTATTNI 217

Query: 191 VESRQQSKLRLR-------------------MYLAGEVTHKGVEAIVSSPRLLKVRSHVT 250
           ++   ++   ++                   ++L G V H     I       KV+ +V 
Sbjct: 218 IDDPNKTTEDIKKKKKVIPPSKTPITEQNRTLHLIGTVEHLDNGGI-------KVKFYVK 277

Query: 251 SSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSISSLPFRDMILAAADKNTGK 310
               D     SL +      +  K+  LST+ RE+ AL+  S   F   ++   D   G+
Sbjct: 278 GIKGDRARQVSLLLRYEIDWWTTKLCNLSTLQREFAALYQCSQSNFMKTLMMRDD--DGE 337

Query: 311 DQ-TWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSAILH 321
           D    KI   L D      N+SQ  A+ + L      LIQGPPGTGKT  ILGL+S +LH
Sbjct: 338 DGIVMKIPPLLHDQFSSTYNDSQLNALTSALEGNAITLIQGPPGTGKTHVILGLISVLLH 378

BLAST of Spg038419 vs. ExPASy Swiss-Prot
Match: O94387 (Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC29A10.10c PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 8.5e-09
Identity = 80/304 (26.32%), Postives = 122/304 (40.13%), Query Frame = 0

Query: 18   FKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDDYISTFEPLLLEEVKAQI 77
            +K+IL W+    L DS            +   + K TY D + Y   F+P+L  E  AQ+
Sbjct: 1063 YKVILGWN---PLADS-----FSASNVEMQCVQAKFTYNDSNAYEKVFKPMLFHECWAQV 1122

Query: 78   IQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDEDSEKGEFLSPNDLMLLSK 137
              ++  EE        I+        +F +I +            +  FLS  D+ LLSK
Sbjct: 1123 --KSAVEEKQYPPIDLILNTRSTVD-NFVDIYF------TSCSPTEVSFLSDTDICLLSK 1182

Query: 138  EKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHKGVEAIVSSPRLLKVRSH 197
                 Q   +   P ++    ++S  + K  L + L        +E+I            
Sbjct: 1183 S----QSSGDTNNPKSFQLCKIQSISRKKESLELCL-----RMNIESIDLQE-------- 1242

Query: 198  VTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSISSLPFRDMILAA----A 257
                     Y  +++ T        K+   +T +RE+ AL S+  LP    IL A     
Sbjct: 1243 ---------YAPNIRFTAQ------KLFNATTSLREFAALKSLRHLPLSQRILDANVTRL 1302

Query: 258  DKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLL 317
              N   D+  KI +         +NE Q  A+ A      F LIQGPPGTGKT+TILG++
Sbjct: 1303 PSNFTDDKKQKIMK------SYGVNEPQAYAIYASSVNDGFTLIQGPPGTGKTKTILGMI 1311

BLAST of Spg038419 vs. ExPASy Swiss-Prot
Match: Q00416 (Helicase SEN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEN1 PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 1.9e-08
Identity = 83/302 (27.48%), Postives = 132/302 (43.71%), Query Frame = 0

Query: 17   LFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDDYISTFEPLLLEEVKAQ 76
            L++IIL WDY R    + +  +D+  G      +VK  +    DY    +PLLL E    
Sbjct: 1116 LYEIILQWDYTR----NSEYPDDEPIG---NYSDVKDFFNSPADYQKVMKPLLLLESWQG 1175

Query: 77   IIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDEDSEKGEFLSPNDLMLLS 136
            +    D E   D+K  +I+  N      F + VY        +D      +S +DL++++
Sbjct: 1176 LCSSRDRE---DYKPFSIIVGNRTAVSDFYD-VYASVAKQVIQDCG----ISESDLIVMA 1235

Query: 137  KEKYSLQFQENAKLPTTYAFALVESRQQSKLR-LRMYLAGEVTHKGVEAIVSSPRLLKVR 196
               Y   F+ + +L ++  F   +    +K+R L+    G V              + +R
Sbjct: 1236 ---YLPDFRPDKRL-SSDDFKKAQHTCLAKVRTLKNTKGGNVD-------------VTLR 1295

Query: 197  SHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSISSLPFRDMILAAADK 256
             H   S       +S  +T+    Y +KV+ ++TI REY  L  +        IL A   
Sbjct: 1296 IHRNHS-------FSKFLTLRSEIYCVKVMQMTTIEREYSTLEGLEYYDLVGQILQAKPS 1355

Query: 257  NTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSA 316
                    +I    + Y    LN SQ  A+   +S++ F LIQGPPGTGKT+TILG++  
Sbjct: 1356 PPVNVDAAEIETVKKSY---KLNTSQAEAIVNSVSKEGFSLIQGPPGTGKTKTILGIIGY 1375

Query: 317  IL 318
             L
Sbjct: 1416 FL 1375

BLAST of Spg038419 vs. ExPASy Swiss-Prot
Match: Q92355 (Helicase sen1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sen1 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.2e-08
Identity = 43/118 (36.44%), Postives = 60/118 (50.85%), Query Frame = 0

Query: 220  YFLKVLLLSTIIREYIALWSISSLPF----RDMILAAADKNTGKDQTWKISRPLQDYMKE 279
            +FLK+  L+T  R+Y     I  LP+     D+I A       K  + +I   ++ Y   
Sbjct: 1075 WFLKLTNLATFTRQYA---GIRGLPYFHLADDIIRARPCSQPVKHSSSEIKAAMKRY--- 1134

Query: 280  NLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLLSAIL------HATPARMHSK 328
             +NE Q  A+   L    F LIQGPPGTGKT+TI+G++SA+L      H T     SK
Sbjct: 1135 QVNEPQAKAIMCALDNNGFTLIQGPPGTGKTKTIIGIISALLVDLSRYHITRPNQQSK 1186

BLAST of Spg038419 vs. ExPASy TrEMBL
Match: A0A6J1F4V5 (probable helicase MAGATAMA 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442358 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 3.1e-155
Identity = 288/327 (88.07%), Postives = 304/327 (92.97%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDD 60
           MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSK++++DDG  A+LGLKEVKS+YKDVDD
Sbjct: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKQKRKDDGGAASLGLKEVKSSYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDED 120
           YISTFEPLLLEE+KAQIIQRNDDE+AADWKFRAIM+C+EVNGFHFPEIVYLRDED+KDED
Sbjct: 61  YISTFEPLLLEEIKAQIIQRNDDEDAADWKFRAIMDCSEVNGFHFPEIVYLRDEDIKDED 120

Query: 121 SEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180
            EK EFLSPNDL+LLSKEK    FQENAKLPTTYAFALVESRQ SKLRLRMYLAGEVTHK
Sbjct: 121 GEKAEFLSPNDLLLLSKEK----FQENAKLPTTYAFALVESRQHSKLRLRMYLAGEVTHK 180

Query: 181 GVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSI 240
           GVEAIVSSPRLLKVRSH+TSSSKDGIYIYS           LK+  LSTIIREYIALWSI
Sbjct: 181 GVEAIVSSPRLLKVRSHITSSSKDGIYIYS-----------LKICSLSTIIREYIALWSI 240

Query: 241 SSLPFRDMILAAADKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGP 300
           SSLPFRDMILAAADKNTG+DQ WKISRPLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGP
Sbjct: 241 SSLPFRDMILAAADKNTGEDQAWKISRPLQDYMRENLNESQQAAVQAGLSRKPFVLIQGP 300

Query: 301 PGTGKTQTILGLLSAILHATPARMHSK 328
           PGTGKTQTILGLLSAILHATPARMHSK
Sbjct: 301 PGTGKTQTILGLLSAILHATPARMHSK 312

BLAST of Spg038419 vs. ExPASy TrEMBL
Match: A0A5A7UB64 (Putative helicase MAGATAMA 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2510G00490 PE=4 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 4.0e-155
Identity = 287/326 (88.04%), Postives = 303/326 (92.94%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDD 60
           MAVDKEK  EESVTSRLFKIILSWDYFRLLK+SKKRKEDDGDGA+LGLKEVKSTYKDVDD
Sbjct: 1   MAVDKEKAVEESVTSRLFKIILSWDYFRLLKNSKKRKEDDGDGASLGLKEVKSTYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDED 120
           Y STFEPLLLEE+KAQIIQRN+DEEA+DWKFRAIMECNEVNGFHFPE+VYLRDEDLKDED
Sbjct: 61  YTSTFEPLLLEEIKAQIIQRNEDEEASDWKFRAIMECNEVNGFHFPEMVYLRDEDLKDED 120

Query: 121 SEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180
           SEKG+FLSPNDL+LLSKEK    FQEN KLPTTYAFALVESRQQSKLRLRMYLAGEVTHK
Sbjct: 121 SEKGDFLSPNDLLLLSKEK----FQENTKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180

Query: 181 GVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSI 240
            VEAI+SSPRLLKV+SH+TSSSKDGIYIYS           LK+  LSTIIREYIALWSI
Sbjct: 181 DVEAIISSPRLLKVQSHITSSSKDGIYIYS-----------LKICSLSTIIREYIALWSI 240

Query: 241 SSLPFRDMILAAADKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGP 300
           SSLPF++MILAAADKNTGKDQ WKIS+PLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGP
Sbjct: 241 SSLPFKEMILAAADKNTGKDQAWKISKPLQDYMQENLNESQQAAVQAGLSRKPFVLIQGP 300

Query: 301 PGTGKTQTILGLLSAILHATPARMHS 327
           PGTGKTQTILGLLSAILHATPARMHS
Sbjct: 301 PGTGKTQTILGLLSAILHATPARMHS 311

BLAST of Spg038419 vs. ExPASy TrEMBL
Match: A0A1S3CEF6 (probable helicase MAGATAMA 3 OS=Cucumis melo OX=3656 GN=LOC103499555 PE=4 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 4.0e-155
Identity = 287/326 (88.04%), Postives = 303/326 (92.94%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDD 60
           MAVDKEK  EESVTSRLFKIILSWDYFRLLK+SKKRKEDDGDGA+LGLKEVKSTYKDVDD
Sbjct: 1   MAVDKEKAVEESVTSRLFKIILSWDYFRLLKNSKKRKEDDGDGASLGLKEVKSTYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDED 120
           Y STFEPLLLEE+KAQIIQRN+DEEA+DWKFRAIMECNEVNGFHFPE+VYLRDEDLKDED
Sbjct: 61  YTSTFEPLLLEEIKAQIIQRNEDEEASDWKFRAIMECNEVNGFHFPEMVYLRDEDLKDED 120

Query: 121 SEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180
           SEKG+FLSPNDL+LLSKEK    FQEN KLPTTYAFALVESRQQSKLRLRMYLAGEVTHK
Sbjct: 121 SEKGDFLSPNDLLLLSKEK----FQENTKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180

Query: 181 GVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSI 240
            VEAI+SSPRLLKV+SH+TSSSKDGIYIYS           LK+  LSTIIREYIALWSI
Sbjct: 181 DVEAIISSPRLLKVQSHITSSSKDGIYIYS-----------LKICSLSTIIREYIALWSI 240

Query: 241 SSLPFRDMILAAADKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGP 300
           SSLPF++MILAAADKNTGKDQ WKIS+PLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGP
Sbjct: 241 SSLPFKEMILAAADKNTGKDQAWKISKPLQDYMQENLNESQQAAVQAGLSRKPFVLIQGP 300

Query: 301 PGTGKTQTILGLLSAILHATPARMHS 327
           PGTGKTQTILGLLSAILHATPARMHS
Sbjct: 301 PGTGKTQTILGLLSAILHATPARMHS 311

BLAST of Spg038419 vs. ExPASy TrEMBL
Match: A0A0A0KM05 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523480 PE=4 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.5e-154
Identity = 286/326 (87.73%), Postives = 302/326 (92.64%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDD 60
           MAVDKEK  EESVTSRLFKIILSWDYFRLLK+SKKRKE+DGDGA+LGLKEVKSTYKDVDD
Sbjct: 1   MAVDKEKAVEESVTSRLFKIILSWDYFRLLKNSKKRKENDGDGASLGLKEVKSTYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDED 120
           Y STFEPLLLEE+KAQIIQRN+DEEA+DWKFRAIMEC+EVNGFHFPE+VYLRDEDLKDED
Sbjct: 61  YTSTFEPLLLEEIKAQIIQRNEDEEASDWKFRAIMECSEVNGFHFPEMVYLRDEDLKDED 120

Query: 121 SEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180
           SEKG+FLSPNDL+LLSKEK    FQEN KLPTTYAFALVESRQQSKLRLRMYLAGEVTHK
Sbjct: 121 SEKGDFLSPNDLLLLSKEK----FQENTKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180

Query: 181 GVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSI 240
            VEAIVSSPRLLKVRSH+TSSSKDGIYIYS           LK+  LSTIIREYIALWSI
Sbjct: 181 DVEAIVSSPRLLKVRSHITSSSKDGIYIYS-----------LKICSLSTIIREYIALWSI 240

Query: 241 SSLPFRDMILAAADKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGP 300
           SSLPF++MILAA DKNTGKDQ WKIS+PLQDYM+ENLNESQQAAVQAGLSRKPFVLIQGP
Sbjct: 241 SSLPFKEMILAATDKNTGKDQAWKISKPLQDYMQENLNESQQAAVQAGLSRKPFVLIQGP 300

Query: 301 PGTGKTQTILGLLSAILHATPARMHS 327
           PGTGKTQTILGLLSAILHATPARMHS
Sbjct: 301 PGTGKTQTILGLLSAILHATPARMHS 311

BLAST of Spg038419 vs. ExPASy TrEMBL
Match: A0A6J1J637 (probable helicase MAGATAMA 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482100 PE=4 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.2e-153
Identity = 285/327 (87.16%), Postives = 302/327 (92.35%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDD 60
           MAVDKEKVAEESVTSRLFKIILSWDYFRLLK+SK++++DDG  A+LGLKEVKS+YKDVDD
Sbjct: 1   MAVDKEKVAEESVTSRLFKIILSWDYFRLLKESKQKRKDDGGAASLGLKEVKSSYKDVDD 60

Query: 61  YISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDED 120
           YISTFEPLLLEE+KAQIIQRNDDE+ ADWKFRAIM+C+EVNGFHFPEIVYLRDED+KDED
Sbjct: 61  YISTFEPLLLEEIKAQIIQRNDDEDVADWKFRAIMDCSEVNGFHFPEIVYLRDEDIKDED 120

Query: 121 SEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVTHK 180
            EK EFLSPNDL+LLSKEK    FQENAKLPTTYAFALVESRQ SKLRLRMYLAGEVTHK
Sbjct: 121 GEKAEFLSPNDLLLLSKEK----FQENAKLPTTYAFALVESRQHSKLRLRMYLAGEVTHK 180

Query: 181 GVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSI 240
           GVEAIVSSPRLLKVRSH+TSSSKDGIYIYS           LK+  LSTIIREYIALWSI
Sbjct: 181 GVEAIVSSPRLLKVRSHITSSSKDGIYIYS-----------LKICSLSTIIREYIALWSI 240

Query: 241 SSLPFRDMILAAADKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLIQGP 300
           SSLPFRDMILAAADKNTG+DQ WKISR LQDYM+ENLNESQQAAVQAGLSRKPFVLIQGP
Sbjct: 241 SSLPFRDMILAAADKNTGEDQAWKISRSLQDYMRENLNESQQAAVQAGLSRKPFVLIQGP 300

Query: 301 PGTGKTQTILGLLSAILHATPARMHSK 328
           PGTGKTQTILGLLSAILHATPARMHSK
Sbjct: 301 PGTGKTQTILGLLSAILHATPARMHSK 312

BLAST of Spg038419 vs. TAIR 10
Match: AT4G15570.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 2.2e-76
Identity = 163/330 (49.39%), Postives = 213/330 (64.55%), Query Frame = 0

Query: 1   MAVDKEKVAEESVTS--RLFKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDV 60
           MA+D  K+ EE  +S  R + IIL WDY +L K+++++   D       L  VK+TYKDV
Sbjct: 1   MAIDNGKLQEEEASSVTRFYNIILGWDYKQLTKENERKNRKDSKEK---LNVVKNTYKDV 60

Query: 61  DDYISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKD 120
           DDY  TFEPLL EEVKAQI+Q  D EEA+  K R +MECNE  GFHF  + Y  +ED   
Sbjct: 61  DDYFETFEPLLFEEVKAQILQNKDGEEASVCKMRLVMECNEGEGFHFLLVTYEHEED--- 120

Query: 121 EDSEKGEFLSPNDLMLLSKEKYSLQFQENAKLPTTYAFALVESRQQSKLRLRMYLAGEVT 180
                 E+L+ NDL+LLSKE+      +    P++Y FA+VE RQ + LRLRMYLA ++ 
Sbjct: 121 ------EYLAQNDLLLLSKEEV-----KGNSFPSSYGFAVVEHRQNNLLRLRMYLAEDIV 180

Query: 181 HKGVEAIVSSPRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALW 240
                 I  + +  + +S + + S     I S    +    + LK+  LSTIIREYIAL 
Sbjct: 181 Q-----ITKNTKSSRTKSFIQALSNMRSLITSSASPIDKRVFSLKLCGLSTIIREYIALR 240

Query: 241 SISSLPFRDMILAAADKNTG-KDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFVLI 300
           S+SSLPF+D+I  AA+K+ G  D+ WKIS PL ++  ENLN+SQ+ A+  GLSRK FVLI
Sbjct: 241 SVSSLPFKDLIFTAAEKSCGFGDEAWKISGPLNEFFNENLNKSQKEAIDVGLSRKSFVLI 300

Query: 301 QGPPGTGKTQTILGLLSAILHATPARMHSK 328
           QGPPGTGKTQTIL +L AI+HATPAR+ SK
Sbjct: 301 QGPPGTGKTQTILSILGAIMHATPARVQSK 308

BLAST of Spg038419 vs. TAIR 10
Match: AT1G16800.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.7e-17
Identity = 88/334 (26.35%), Postives = 150/334 (44.91%), Query Frame = 0

Query: 18   FKIILSWDYFRLLKDSKKRKEDDGDGAALGLKEVKSTYKDVDDYISTFEPLLLEEVKAQI 77
            F+ IL  DY+ ++  +  +K++  +      +EV   +   + YI  F+PL+LEE KAQ+
Sbjct: 1058 FRKILQMDYYAIVGLASTKKDESQNVGK--FREVPVRFGSPEQYIQIFQPLVLEEFKAQL 1117

Query: 78   ---IQRNDDEEAADWKFRAIMECNEVNGFHFPEIVYLRDEDLKDEDSEKGEFLSPNDLML 137
                Q     E   +   +++    V+ FHF  + +++DE+    D    +  S NDL+L
Sbjct: 1118 QSSFQEISSLEEIYYGVISVLSIERVDDFHF--VRFMQDEN----DGSNSKSFSENDLVL 1177

Query: 138  LSKEKYSLQFQENAKLPTTYAFALVESRQ------QSKLRLRMYLAGEVTHKGVEAIVSS 197
             +KE       EN+ +        VE R+       S L +R+YL             +S
Sbjct: 1178 FTKE-----HPENSNVGVN-MMGKVEGREWDDKKRTSILNVRLYLQN-----------AS 1237

Query: 198  PRLLKVRSHVTSSSKDGIYIYSLKVTVSPTSYFLKVLLLSTIIREYIALWSISSLPFRDM 257
             RL + R ++   S+                +  ++L +++ IRE+ AL  I  +P   +
Sbjct: 1238 SRLNQARRNLLERSQ---------------WHASRILNITSQIREFQALSCIKDIPVLPL 1297

Query: 258  ILAAA-----DKNTGKDQTWKISRPLQDYMKENLNESQQAAVQAGLSRKPFV------LI 317
            IL+       D    +     +   LQ  +K + NESQ  A+   +     +      LI
Sbjct: 1298 ILSPMNDSNYDSEVKRSDLRSLPHSLQQILKSSFNESQLQAISVAIGSSNLMKAFDISLI 1351

Query: 318  QGPPGTGKTQTILGLLSAIL----HATPARMHSK 328
            QGPPGTGKT+TI+ ++S +L    H T  R +S+
Sbjct: 1358 QGPPGTGKTRTIVAIISGLLASASHKTSDRGNSE 1351

BLAST of Spg038419 vs. TAIR 10
Match: AT2G19120.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-06
Identity = 78/297 (26.26%), Postives = 128/297 (43.10%), Query Frame = 0

Query: 48  LKEVKSTYKDVDDYISTFEPLLLEEVKAQIIQRNDDEEAADWKFRAIMECNEVNGFHFPE 107
           L+ V   ++ +++Y+  FEPLL EE +AQ+             +   +E  E N      
Sbjct: 254 LRSVPERFESMEEYVRVFEPLLFEECRAQL-------------YSTWVELAEAN------ 313

Query: 108 IVYLRDEDLKDEDSEKGEFLSPNDLMLLSKEKYSLQFQEN--AKLPTTYAFALVESRQQS 167
             Y++      E  E+G +    D++L S       F+E   A L T     L ES +  
Sbjct: 314 -TYVKVRIKSIERRERGWY----DVILNSLNGCKWAFKEGDVAVLST----PLPESDEDH 373

Query: 168 KLRLRMYLAGEVTHKGVEAIVSSPRLLKVRSHVTSSSKDGIYI---YSLKVTVSPTSYFL 227
           +   R  +AG V  + +      PR   +  +V +S   G  I   + L+       + L
Sbjct: 374 EDAGR--VAGTV-RRHIPVDTRDPRGATLHFYVGNSGGTGSKIDDSHILRKLKPQDIWHL 433

Query: 228 KVL-LLSTIIREYIALWSISSL--PFRDMILAAADKNTGKDQTWKISRP------LQDYM 287
            VL  L+T  REY+AL + S L    ++ IL  + +     +    + P        D++
Sbjct: 434 TVLGSLATTQREYVALHAFSRLNPQMQNAILNPSPEQFPSYEEQTPATPDCFTTSFVDHL 493

Query: 288 KENLNESQQAAV-----------QAGLSRK---PFVLIQGPPGTGKTQTILGLLSAI 317
             + N  Q AA+            +G+ ++   PF L+QGPPGTGKT T+ G+L+ I
Sbjct: 494 HRSFNAPQLAAIHWAAMHTAAGTSSGVKKQEPWPFTLVQGPPGTGKTHTVWGMLNVI 519

BLAST of Spg038419 vs. TAIR 10
Match: AT4G30100.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 42.7 bits (99), Expect = 6.4e-04
Identity = 19/37 (51.35%), Postives = 26/37 (70.27%), Query Frame = 0

Query: 283 AAVQAGLSRK---PFVLIQGPPGTGKTQTILGLLSAI 317
           A   +G+ R+   PF L+QGPPGTGKT T+ G+L+ I
Sbjct: 647 AGTSSGVKRQDPWPFTLVQGPPGTGKTHTVWGMLNVI 683

BLAST of Spg038419 vs. TAIR 10
Match: AT2G03270.1 (DNA-binding protein, putative )

HSP 1 Score: 42.4 bits (98), Expect = 8.4e-04
Identity = 18/39 (46.15%), Postives = 28/39 (71.79%), Query Frame = 0

Query: 275 ENLNESQQAAVQAGLSRKPFVLIQGPPGTGKTQTILGLL 314
           +NL++SQ+ A+   LS K   L+ GPPGTGKT T++ ++
Sbjct: 188 KNLDQSQKDAITKALSSKDVFLLHGPPGTGKTTTVVEIV 226

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038881790.12.2e-15588.99probable helicase MAGATAMA 3 isoform X2 [Benincasa hispida] >XP_038881791.1 prob... [more]
KAG6581597.16.4e-15588.07putative helicase MAGATAMA 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023528758.16.4e-15588.07probable helicase MAGATAMA 3 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7018098.16.4e-15588.07putative helicase MAGATAMA 3, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022935506.16.4e-15588.07probable helicase MAGATAMA 3 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
B6SFA43.0e-7549.39Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1[more]
Q86AS02.9e-1726.80Probable helicase DDB_G0274399 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274... [more]
O943878.5e-0926.32Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (... [more]
Q004161.9e-0827.48Helicase SEN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292... [more]
Q923553.2e-0836.44Helicase sen1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 G... [more]
Match NameE-valueIdentityDescription
A0A6J1F4V53.1e-15588.07probable helicase MAGATAMA 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A5A7UB644.0e-15588.04Putative helicase MAGATAMA 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CEF64.0e-15588.04probable helicase MAGATAMA 3 OS=Cucumis melo OX=3656 GN=LOC103499555 PE=4 SV=1[more]
A0A0A0KM054.5e-15487.73Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523480 PE=4 SV=1[more]
A0A6J1J6372.2e-15387.16probable helicase MAGATAMA 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482... [more]
Match NameE-valueIdentityDescription
AT4G15570.12.2e-7649.39P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G16800.11.7e-1726.35P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G19120.11.1e-0626.26P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT4G30100.16.4e-0451.35P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G03270.18.4e-0446.15DNA-binding protein, putative [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041677DNA2/NAM7 helicase, helicase domainPFAMPF13086AAA_11coord: 276..317
e-value: 4.9E-11
score: 42.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 228..325
e-value: 1.7E-16
score: 62.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 273..319
NoneNo IPR availablePANTHERPTHR10887:SF489BNAC01G22370D PROTEINcoord: 11..325
IPR045055DNA2/NAM7-like helicasePANTHERPTHR10887DNA2/NAM7 HELICASE FAMILYcoord: 11..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg038419.1Spg038419.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004386 helicase activity
molecular_function GO:0003723 RNA binding