Spg038193 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg038193
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionphospholipase A1-Ibeta2, chloroplastic
Locationscaffold12: 39840425 .. 39841930 (+)
RNA-Seq ExpressionSpg038193
SyntenySpg038193
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAATTAATCAGTTCCACAGTTCCGGCGCAAAATCTCTCCGGCTATCGGGTCCGCCGATCGACTTTCAAGTACCAAATGTCGCCGTTGAACCCGTCGGCGGCGAATATCTCTTCATTTAAACCGATGCCGGTTGATGGGACTCGCCGGCATCTGGCGAATTTGGATAAGCTGTTGAAGAAAACAGACCCCCCGGCGAAGTTGGAGCCGGAACCGGTTCACAAGGACCCGAACAAGGGATCGGGATCGTCGATGCCGATCAGAGGGAAGGGGCTTTTGGAAGGCTTGAATCTGGCGAGAATCTGGCCGGAGATGAAGGCGGCGGAGGAAATGTCGCCGAGAAACCTGAACCGGTTGCAGCGGCTGCTGTCGAAGACGGTGGAGTATTCGCCGAGAAACGCCCTCGGCCGGCGGTGGCGCGAGTACCACGGCTGTAACGACTGGTCCGGTTTGCTCGACCCGTTGGATGAAAATCTCCGCCGTGAAGTCGTCCGGTACGGCGAGTTCGTACAAGCCGCGTACCATTCTTTCCATTCAAATCCCTCCACGTCGCCCAACGAGGCCCCACTCCCTCGCCACGTGGCGCTCCCAGATAGGTCTTACAAAGTCACGAAGAGTCTCTACGCCACGTCATCGGTCGGTCTACCCAGTTGGGTCGACGACGTGGCTCCCGATCTCGGCTGGATGACCCAACGGTCCAGTTGGGTCGGATACGTCGCCGTTTGCGACGACCGCCGTGAAATCGCCCGTATGGGGCGGCGCGATATCGTCATCGCCCTCCGTGGCACCGCCACCTGTCTCGAATGGGCCGAGAACGTCAGGGCTCATCTCATCGACATCCCCGCCGACGACGCCGCCGCCGCCGTCGACTGCGGAGATCCGAAAGTCGAGTGCGGATTTCTCAGCCTCTACAAAACCGCCGGAGCGCACGTCAAGAGCTTATCGGAGTCAGTGGTGGAAGAGATCCGTCGATTGACGGAGATCTACAAAGGAGAAACGCTGAGCATCACAGTCACCGGACACAGCCTCGGCGCGGCATTGGCTCTTCTCGTGGCCGACGAGATCAGCGTCTGTGCACCGGAGGTTCCTCCGGTGGCGGTGTTCTCCTTCGGCGGACCGCGCGTGGGGAACAGAATCTTCGCGGATCGAATCAGATCTCGAAACGTGAAAGTACTGCGGATCGTGAACTCGCAAGACGTGATAACGCAGGTTCCGCCAGTGACGTACTCGCACGTGGGAACGGAGCTGCGAGTGGAGACGAAGATGTCGCCGTACCTGAAACCTAACGCCGACGTCGCGTGCTGCCATGATCTGGAAGCGTATCTGCATCTGGTGGACGGATTCTTGTCGTCGAAATGTCCGTTCCGGCCGAACGCGAAACGGAGCTTGGCGCGATTGGTGCAGGATCAGAGAGGGAATGTGAAGAAACTGTACATGAGGAAGGTGAAGGATTTGGGGTTCAATCCGGAGCTGCAGACGGCGGGGTGTCTGCCAAGTCCGTCGTGA

mRNA sequence

ATGCAATTAATCAGTTCCACAGTTCCGGCGCAAAATCTCTCCGGCTATCGGGTCCGCCGATCGACTTTCAAGTACCAAATGTCGCCGTTGAACCCGTCGGCGGCGAATATCTCTTCATTTAAACCGATGCCGGTTGATGGGACTCGCCGGCATCTGGCGAATTTGGATAAGCTGTTGAAGAAAACAGACCCCCCGGCGAAGTTGGAGCCGGAACCGGTTCACAAGGACCCGAACAAGGGATCGGGATCGTCGATGCCGATCAGAGGGAAGGGGCTTTTGGAAGGCTTGAATCTGGCGAGAATCTGGCCGGAGATGAAGGCGGCGGAGGAAATGTCGCCGAGAAACCTGAACCGGTTGCAGCGGCTGCTGTCGAAGACGGTGGAGTATTCGCCGAGAAACGCCCTCGGCCGGCGGTGGCGCGAGTACCACGGCTGTAACGACTGGTCCGGTTTGCTCGACCCGTTGGATGAAAATCTCCGCCGTGAAGTCGTCCGGTACGGCGAGTTCGTACAAGCCGCGTACCATTCTTTCCATTCAAATCCCTCCACGTCGCCCAACGAGGCCCCACTCCCTCGCCACGTGGCGCTCCCAGATAGGTCTTACAAAGTCACGAAGAGTCTCTACGCCACGTCATCGGTCGGTCTACCCAGTTGGGTCGACGACGTGGCTCCCGATCTCGGCTGGATGACCCAACGGTCCAGTTGGGTCGGATACGTCGCCGTTTGCGACGACCGCCGTGAAATCGCCCGTATGGGGCGGCGCGATATCGTCATCGCCCTCCGTGGCACCGCCACCTGTCTCGAATGGGCCGAGAACGTCAGGGCTCATCTCATCGACATCCCCGCCGACGACGCCGCCGCCGCCGTCGACTGCGGAGATCCGAAAGTCGAGTGCGGATTTCTCAGCCTCTACAAAACCGCCGGAGCGCACGTCAAGAGCTTATCGGAGTCAGTGGTGGAAGAGATCCGTCGATTGACGGAGATCTACAAAGGAGAAACGCTGAGCATCACAGTCACCGGACACAGCCTCGGCGCGGCATTGGCTCTTCTCGTGGCCGACGAGATCAGCGTCTGTGCACCGGAGGTTCCTCCGGTGGCGGTGTTCTCCTTCGGCGGACCGCGCGTGGGGAACAGAATCTTCGCGGATCGAATCAGATCTCGAAACGTGAAAGTACTGCGGATCGTGAACTCGCAAGACGTGATAACGCAGGTTCCGCCAGTGACGTACTCGCACGTGGGAACGGAGCTGCGAGTGGAGACGAAGATGTCGCCGTACCTGAAACCTAACGCCGACGTCGCGTGCTGCCATGATCTGGAAGCGTATCTGCATCTGGTGGACGGATTCTTGTCGTCGAAATGTCCGTTCCGGCCGAACGCGAAACGGAGCTTGGCGCGATTGGTGCAGGATCAGAGAGGGAATGTGAAGAAACTGTACATGAGGAAGGTGAAGGATTTGGGGTTCAATCCGGAGCTGCAGACGGCGGGGTGTCTGCCAAGTCCGTCGTGA

Coding sequence (CDS)

ATGCAATTAATCAGTTCCACAGTTCCGGCGCAAAATCTCTCCGGCTATCGGGTCCGCCGATCGACTTTCAAGTACCAAATGTCGCCGTTGAACCCGTCGGCGGCGAATATCTCTTCATTTAAACCGATGCCGGTTGATGGGACTCGCCGGCATCTGGCGAATTTGGATAAGCTGTTGAAGAAAACAGACCCCCCGGCGAAGTTGGAGCCGGAACCGGTTCACAAGGACCCGAACAAGGGATCGGGATCGTCGATGCCGATCAGAGGGAAGGGGCTTTTGGAAGGCTTGAATCTGGCGAGAATCTGGCCGGAGATGAAGGCGGCGGAGGAAATGTCGCCGAGAAACCTGAACCGGTTGCAGCGGCTGCTGTCGAAGACGGTGGAGTATTCGCCGAGAAACGCCCTCGGCCGGCGGTGGCGCGAGTACCACGGCTGTAACGACTGGTCCGGTTTGCTCGACCCGTTGGATGAAAATCTCCGCCGTGAAGTCGTCCGGTACGGCGAGTTCGTACAAGCCGCGTACCATTCTTTCCATTCAAATCCCTCCACGTCGCCCAACGAGGCCCCACTCCCTCGCCACGTGGCGCTCCCAGATAGGTCTTACAAAGTCACGAAGAGTCTCTACGCCACGTCATCGGTCGGTCTACCCAGTTGGGTCGACGACGTGGCTCCCGATCTCGGCTGGATGACCCAACGGTCCAGTTGGGTCGGATACGTCGCCGTTTGCGACGACCGCCGTGAAATCGCCCGTATGGGGCGGCGCGATATCGTCATCGCCCTCCGTGGCACCGCCACCTGTCTCGAATGGGCCGAGAACGTCAGGGCTCATCTCATCGACATCCCCGCCGACGACGCCGCCGCCGCCGTCGACTGCGGAGATCCGAAAGTCGAGTGCGGATTTCTCAGCCTCTACAAAACCGCCGGAGCGCACGTCAAGAGCTTATCGGAGTCAGTGGTGGAAGAGATCCGTCGATTGACGGAGATCTACAAAGGAGAAACGCTGAGCATCACAGTCACCGGACACAGCCTCGGCGCGGCATTGGCTCTTCTCGTGGCCGACGAGATCAGCGTCTGTGCACCGGAGGTTCCTCCGGTGGCGGTGTTCTCCTTCGGCGGACCGCGCGTGGGGAACAGAATCTTCGCGGATCGAATCAGATCTCGAAACGTGAAAGTACTGCGGATCGTGAACTCGCAAGACGTGATAACGCAGGTTCCGCCAGTGACGTACTCGCACGTGGGAACGGAGCTGCGAGTGGAGACGAAGATGTCGCCGTACCTGAAACCTAACGCCGACGTCGCGTGCTGCCATGATCTGGAAGCGTATCTGCATCTGGTGGACGGATTCTTGTCGTCGAAATGTCCGTTCCGGCCGAACGCGAAACGGAGCTTGGCGCGATTGGTGCAGGATCAGAGAGGGAATGTGAAGAAACTGTACATGAGGAAGGTGAAGGATTTGGGGTTCAATCCGGAGCTGCAGACGGCGGGGTGTCTGCCAAGTCCGTCGTGA

Protein sequence

MQLISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFKPMPVDGTRRHLANLDKLLKKTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAAEEMSPRNLNRLQRLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSNPSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVECGFLSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISVCAPEVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVPPVTYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQRGNVKKLYMRKVKDLGFNPELQTAGCLPSPS
Homology
BLAST of Spg038193 vs. NCBI nr
Match: XP_038887009.1 (phospholipase A1-Ibeta2, chloroplastic [Benincasa hispida])

HSP 1 Score: 935.3 bits (2416), Expect = 2.3e-268
Identity = 461/503 (91.65%), Postives = 483/503 (96.02%), Query Frame = 0

Query: 1   MQLISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFKP--MPVDGTRRHLANLDKL 60
           MQ+ISST+PAQNLS ++VRRS+FKYQMSPLNPSAA ISSFKP    VDG RRHLANLDKL
Sbjct: 1   MQIISSTIPAQNLSQFQVRRSSFKYQMSPLNPSAAQISSFKPATSTVDGARRHLANLDKL 60

Query: 61  LKKTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAAEEMSPRNLNR 120
           LKKTDPP K++PEPVHKDPNK  GS MPI+GKGLLEGLNLARIWPE+KA EEMSPRNLNR
Sbjct: 61  LKKTDPPVKVDPEPVHKDPNK--GSVMPIKGKGLLEGLNLARIWPEVKAVEEMSPRNLNR 120

Query: 121 LQRLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFH 180
           LQRLLSKT+EYSPRNALG+RWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYH+FH
Sbjct: 121 LQRLLSKTMEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFH 180

Query: 181 SNPSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGY 240
           SNP+TSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLP WVD+VAPDLGWMTQRSSWVGY
Sbjct: 181 SNPATSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGY 240

Query: 241 VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVEC 300
           VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRA LI+IPAD  AAA D GDPKVEC
Sbjct: 241 VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDPKVEC 300

Query: 301 GFLSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISVC 360
           GFLSLYKTAGAHVKSLSESVVEEIRRLTE+YKGE LSITVTGHSLGAALA+LVADEISVC
Sbjct: 301 GFLSLYKTAGAHVKSLSESVVEEIRRLTEMYKGERLSITVTGHSLGAALAVLVADEISVC 360

Query: 361 APEVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVPPVTYSHVGTELRV 420
           +PEVPPVAVFSFGGPRVGN+IFA+RI+SRNVKVLRIVNSQDVITQVPPVTYSHVGTELRV
Sbjct: 361 SPEVPPVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVPPVTYSHVGTELRV 420

Query: 421 ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQRGNVKKLY 480
           ETKMSP+LKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSL RLVQDQRGNVKKLY
Sbjct: 421 ETKMSPFLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLVRLVQDQRGNVKKLY 480

Query: 481 MRKVKDLGFNPELQTAGCLPSPS 502
           MRKVKDLGFNPELQTAGCLPSPS
Sbjct: 481 MRKVKDLGFNPELQTAGCLPSPS 501

BLAST of Spg038193 vs. NCBI nr
Match: XP_008465868.2 (PREDICTED: phospholipase A1-Ibeta2, chloroplastic [Cucumis melo] >TYK31086.1 phospholipase A1-Ibeta2 [Cucumis melo var. makuwa])

HSP 1 Score: 894.4 bits (2310), Expect = 4.4e-256
Identity = 438/503 (87.08%), Postives = 471/503 (93.64%), Query Frame = 0

Query: 3   LISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFK--PMPVDGTRRHLANLDKLLK 62
           +I+ST+P QNLS + +RRS+FKYQ+SPLNPSA  ISSF+   + V  TRRHLANLDKLL 
Sbjct: 4   VINSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLN 63

Query: 63  KTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAAEEMSPRNLNRLQ 122
           KTDPP K++P+PVHKDPNKGS   MPI+GKGLLEGLNL+R+WPE+KA EEMSPRNLNRLQ
Sbjct: 64  KTDPPLKVDPQPVHKDPNKGS-LHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQ 123

Query: 123 RLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSN 182
           RLLSKTVEYSPRNALG+RWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYH+FHSN
Sbjct: 124 RLLSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSN 183

Query: 183 PSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVA 242
           P+TSPNE PLPRHV LPDRSYKVTKSLYATSSVGLP W+D+VAPDLGWMTQRSSWVGYVA
Sbjct: 184 PTTSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVA 243

Query: 243 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVECGF 302
           VCDDRREIARMGRRDIVIALRGTATCLEWAENVRA LIDIPAD      DCGDPKVECGF
Sbjct: 244 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPAD--VDTKDCGDPKVECGF 303

Query: 303 LSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISVCAP 362
           LSLYKTAGAHV+SLSESVVEEIRRLTE+YKGETLSITVTGHSLGAALA+LVADEISVC+ 
Sbjct: 304 LSLYKTAGAHVRSLSESVVEEIRRLTEMYKGETLSITVTGHSLGAALAILVADEISVCSA 363

Query: 363 EVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP--PVTYSHVGTELRV 422
           EVPPVAVFSFGGPRVGN++FADRI+SRNVKVLRIVNSQDVITQVP  P+TYSHVGTELRV
Sbjct: 364 EVPPVAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRV 423

Query: 423 ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQRGNVKKLY 482
           ETKMSP+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQRGN+KKLY
Sbjct: 424 ETKMSPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLY 483

Query: 483 MRKVKDLGFNPELQTAGCLPSPS 502
           MRKVKDLGFNPELQT GCLPSPS
Sbjct: 484 MRKVKDLGFNPELQTVGCLPSPS 503

BLAST of Spg038193 vs. NCBI nr
Match: XP_023554175.1 (phospholipase A1-Ibeta2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 893.6 bits (2308), Expect = 7.5e-256
Identity = 445/503 (88.47%), Postives = 466/503 (92.64%), Query Frame = 0

Query: 1   MQLISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFKP--MPVDGTRRHLANLDKL 60
           MQ+I+ST+PAQNLS ++VRRS+FKYQMSPLNPSAA IS F+P    VDGTRRHLANLDKL
Sbjct: 1   MQIINSTIPAQNLSRFQVRRSSFKYQMSPLNPSAAKISYFRPATAAVDGTRRHLANLDKL 60

Query: 61  LKKTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAAEEMSPRNLNR 120
           LKKTD PAK+EPEPV KDPNK  GS MPI+GKGLLEGLNLARIWPEM+AAE+MSPRNLNR
Sbjct: 61  LKKTDTPAKVEPEPVFKDPNK--GSVMPIKGKGLLEGLNLARIWPEMRAAEDMSPRNLNR 120

Query: 121 LQRLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFH 180
           LQRLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQ+AYHSFH
Sbjct: 121 LQRLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQSAYHSFH 180

Query: 181 SNPSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGY 240
           SNPS S NEAPLPRHVALPD+SYKVTKSLYATSSVGLP WVD+VAPDLGWMTQRSSW+GY
Sbjct: 181 SNPSMSLNEAPLPRHVALPDKSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWMGY 240

Query: 241 VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVEC 300
           VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRA L +IP D      D GDPKVEC
Sbjct: 241 VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLTNIPTD-----TDSGDPKVEC 300

Query: 301 GFLSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISVC 360
           GFLSLYKTAGAHVKSLSESVVEEIRRL EIYKGETLSI+VTGHSLGAALALLVADEISVC
Sbjct: 301 GFLSLYKTAGAHVKSLSESVVEEIRRLMEIYKGETLSISVTGHSLGAALALLVADEISVC 360

Query: 361 APEVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVPPVTYSHVGTELRV 420
           +P VPPVAVFSFGGPRVGN  FA+RI SRNVKVLRIVNSQD+ITQVPPVTYSHVGTELRV
Sbjct: 361 SPTVPPVAVFSFGGPRVGNTTFANRITSRNVKVLRIVNSQDLITQVPPVTYSHVGTELRV 420

Query: 421 ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQRGNVKKLY 480
           ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFR NAKRSL RLV DQRGN+KKLY
Sbjct: 421 ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRANAKRSLVRLVHDQRGNMKKLY 480

Query: 481 MRKVKDLGFNPELQTAGCLPSPS 502
           MRKVKDL  NPELQ  GCLPSPS
Sbjct: 481 MRKVKDLSLNPELQMLGCLPSPS 496

BLAST of Spg038193 vs. NCBI nr
Match: KAA0038492.1 (phospholipase A1-Ibeta2 [Cucumis melo var. makuwa])

HSP 1 Score: 892.9 bits (2306), Expect = 1.3e-255
Identity = 437/503 (86.88%), Postives = 471/503 (93.64%), Query Frame = 0

Query: 3   LISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFK--PMPVDGTRRHLANLDKLLK 62
           +I+ST+P QNLS + +RRS+FKYQ+SPLNPSA  ISSF+   + V  TRRHLANLDKLL 
Sbjct: 4   VINSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLN 63

Query: 63  KTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAAEEMSPRNLNRLQ 122
           KTDPP K++P+PVHKDPNKGS   MPI+GKGLLEGLNL+R+WPE+KA EEMSPRNLNRLQ
Sbjct: 64  KTDPPLKVDPQPVHKDPNKGS-LHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQ 123

Query: 123 RLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSN 182
           RLLSKTVEYSPRNALG+RWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYH+FHSN
Sbjct: 124 RLLSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSN 183

Query: 183 PSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVA 242
           P+TSPNE PLPRHV LPDRSYKVTKSLYATSSVGLP W+D+VAPDLGWMTQRSSWVGYVA
Sbjct: 184 PTTSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVA 243

Query: 243 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVECGF 302
           VCDDRREIARMGRRDIVIALRGTATCLEWAENVRA LIDIPAD      DCGDPKVECGF
Sbjct: 244 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPAD--VDTKDCGDPKVECGF 303

Query: 303 LSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISVCAP 362
           LSLYKTAGAHV+SLSESVVEEIR+LTE+YKGETLSITVTGHSLGAALA+LVADEISVC+ 
Sbjct: 304 LSLYKTAGAHVRSLSESVVEEIRQLTEMYKGETLSITVTGHSLGAALAILVADEISVCSA 363

Query: 363 EVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP--PVTYSHVGTELRV 422
           EVPPVAVFSFGGPRVGN++FADRI+SRNVKVLRIVNSQDVITQVP  P+TYSHVGTELRV
Sbjct: 364 EVPPVAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRV 423

Query: 423 ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQRGNVKKLY 482
           ETKMSP+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQRGN+KKLY
Sbjct: 424 ETKMSPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLY 483

Query: 483 MRKVKDLGFNPELQTAGCLPSPS 502
           MRKVKDLGFNPELQT GCLPSPS
Sbjct: 484 MRKVKDLGFNPELQTVGCLPSPS 503

BLAST of Spg038193 vs. NCBI nr
Match: XP_022931784.1 (phospholipase A1-Ibeta2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 888.3 bits (2294), Expect = 3.2e-254
Identity = 442/503 (87.87%), Postives = 465/503 (92.45%), Query Frame = 0

Query: 1   MQLISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFKP--MPVDGTRRHLANLDKL 60
           MQ+I+ST+PAQNLS ++VRRS+FKYQMSPLNPSAA IS F+P    VDGTRRHLANLDKL
Sbjct: 1   MQIINSTIPAQNLSRFQVRRSSFKYQMSPLNPSAAKISYFRPATAAVDGTRRHLANLDKL 60

Query: 61  LKKTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAAEEMSPRNLNR 120
           LKKTD PAK+EPEPV KDPNK  GS MPI+GKGLLEGLNLARIWPEM+AAE+MSPRNLNR
Sbjct: 61  LKKTDTPAKVEPEPVFKDPNK--GSVMPIKGKGLLEGLNLARIWPEMRAAEDMSPRNLNR 120

Query: 121 LQRLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFH 180
           LQRLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQ+AYHSFH
Sbjct: 121 LQRLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQSAYHSFH 180

Query: 181 SNPSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGY 240
           SNP+ S NEAPLPRHVALPD+SYKVTKSLYATSSVGLP WVD+VAPDLGWMTQRSSW+GY
Sbjct: 181 SNPAMSLNEAPLPRHVALPDKSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWMGY 240

Query: 241 VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVEC 300
           VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRA L +IP D      +  DPKVEC
Sbjct: 241 VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLTNIPTD-----TNSSDPKVEC 300

Query: 301 GFLSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISVC 360
           GFLSLYKTAGAHVKSLSESVVEEIRRL EIYKGETLSI+VTGHSLGAALALLVADEISVC
Sbjct: 301 GFLSLYKTAGAHVKSLSESVVEEIRRLMEIYKGETLSISVTGHSLGAALALLVADEISVC 360

Query: 361 APEVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVPPVTYSHVGTELRV 420
           +P VPPVAVFSFGGPRVGN  FA+RI SRNVKVLRIVNSQD+ITQVPPVTYSHVGTELRV
Sbjct: 361 SPTVPPVAVFSFGGPRVGNTAFANRITSRNVKVLRIVNSQDLITQVPPVTYSHVGTELRV 420

Query: 421 ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQRGNVKKLY 480
           ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFR NAKRSL RLV DQRGN+KKLY
Sbjct: 421 ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRANAKRSLVRLVHDQRGNMKKLY 480

Query: 481 MRKVKDLGFNPELQTAGCLPSPS 502
           MRKVKDL  NPELQ  GCLPSPS
Sbjct: 481 MRKVKDLSLNPELQMLGCLPSPS 496

BLAST of Spg038193 vs. ExPASy Swiss-Prot
Match: O23522 (Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16820 PE=1 SV=2)

HSP 1 Score: 551.6 bits (1420), Expect = 9.2e-156
Identity = 305/535 (57.01%), Postives = 363/535 (67.85%), Query Frame = 0

Query: 4   ISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFKPMPVDGTRR----HLANLDKLL 63
           + +  P  ++   + RR T     S L P+       KP+ V   R+    HL NL+ +L
Sbjct: 1   MQTLTPNADIFHAKRRRFTCNTHTSTLIPT-------KPLSVSPARKTNKEHLRNLENVL 60

Query: 64  KKTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAA-EEMSPRNLNR 123
           + +        + +    ++   ++       LL GLNLARIWP+MKAA +EMSP+NL R
Sbjct: 61  RTSSNSI----DHIENVTSRQEKTTKNTSTSSLLGGLNLARIWPQMKAAVDEMSPKNLKR 120

Query: 124 LQRLLSK-TVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSF 183
           LQRLLSK + E SP++ LG +WRE HG N+W+GLLDPLDENLRRE+VRYGEFVQAAYH+F
Sbjct: 121 LQRLLSKSSEERSPKSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAF 180

Query: 184 HSNPSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVG 243
           HS+P  S      PRHVALPD S+KVTKSLYATSSV LP W+DDVAPDL WMT+++SWVG
Sbjct: 181 HSDPEGS------PRHVALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVG 240

Query: 244 YVAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVE 303
           YVAVCDD REI RMGRR+IVIALRGTAT LEW+EN R +L+ +P +      D   PKVE
Sbjct: 241 YVAVCDDPREIRRMGRREIVIALRGTATLLEWSENFRPNLVSMP-EPKPDQSDPTRPKVE 300

Query: 304 CGFLSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISV 363
           CGF SLY T   H  SL+ES+V EI RL E+Y GE LSI+VTGHSLGAA+ALL AD+I+ 
Sbjct: 301 CGFNSLYTTGDQHAPSLAESLVGEISRLVELYAGEELSISVTGHSLGAAIALLAADDIAE 360

Query: 364 CAPEVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP------------ 423
             P  PPVAVFSFGGPRVGNR FADR+ S+ VKVLR+VNSQDV+T+VP            
Sbjct: 361 RVPHAPPVAVFSFGGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQS 420

Query: 424 ------------------PVTYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDG 483
                             P  YSHVG ELRV+ KMSPYLKPNADVACCHDLEAYLHLVDG
Sbjct: 421 RNNGRSPGGIMEMVERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDG 480

Query: 484 FLSSKCPFRPNAKRSLARLVQDQRGNVKKLYMRKVKDLGFNPELQTAG-CLPSPS 502
           FL+S CPFR NAKRSL +L+ +QR NVK LY    K L  N      G  LPSPS
Sbjct: 481 FLASNCPFRANAKRSLRKLLDEQRSNVKVLYTG--KSLRLNRTFHDNGDVLPSPS 515

BLAST of Spg038193 vs. ExPASy Swiss-Prot
Match: Q948R1 (Phospholipase A(1) DAD1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAD1 PE=1 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 2.3e-90
Identity = 185/382 (48.43%), Postives = 238/382 (62.30%), Query Frame = 0

Query: 128 EYSPRNA-----LGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSNPS 187
           E+SP  +     LGRRW EY G  +W GLLDPLD+NLRRE++RYG+FV++AY +F  +PS
Sbjct: 74  EFSPTGSSIPVKLGRRWMEYQGLQNWDGLLDPLDDNLRREILRYGQFVESAYQAFDFDPS 133

Query: 188 TS-------PNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSW 247
           +        P    L R   LP+  Y++TK+L ATS + LP W++  AP   WM  +SSW
Sbjct: 134 SPTYGTCRFPRSTLLERS-GLPNSGYRLTKNLRATSGINLPRWIEK-AP--SWMATQSSW 193

Query: 248 VGYVAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGD-- 307
           +GYVAVC D+ EI+R+GRRD+VI+ RGTATCLEW EN+RA L  +P     A ++  +  
Sbjct: 194 IGYVAVCQDKEEISRLGRRDVVISFRGTATCLEWLENLRATLTHLPNGPTGANLNGSNSG 253

Query: 308 PKVECGFLSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVAD 367
           P VE GFLSLY T+G H  SL + V EEI RL + Y  E LS+T+TGHSLGAA+A L A 
Sbjct: 254 PMVESGFLSLY-TSGVH--SLRDMVREEIARLLQSYGDEPLSVTITGHSLGAAIATLAAY 313

Query: 368 EISVCAPEVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP-------- 427
           +I       P V V SFGGPRVGNR F   +  +  KVLRIVNS DVIT+VP        
Sbjct: 314 DIKTTFKRAPMVTVISFGGPRVGNRCFRKLLEKQGTKVLRIVNSDDVITKVPGVVLENRE 373

Query: 428 ----------------------PVTYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLH 466
                                 P  Y+ +G ELR+ ++ SP+L  + +VA CH+L+ YLH
Sbjct: 374 QDNVKMTASIMPSWIQRRVEETPWVYAEIGKELRLSSRDSPHLS-SINVATCHELKTYLH 433

BLAST of Spg038193 vs. ExPASy Swiss-Prot
Match: Q9C8J6 (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2 PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.8e-64
Identity = 148/356 (41.57%), Postives = 201/356 (56.46%), Query Frame = 0

Query: 139 WREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSNPSTS--------PNEAPL 198
           WRE  GCN+W G LDP++ +LRRE++RYGEF QA Y SF  +P +         P++  L
Sbjct: 91  WREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPSDFFL 150

Query: 199 PRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVAVCDDRREIAR 258
              + L  + Y +T+ LYATS++ LP++         W +Q ++W+G+VAV  D  E++R
Sbjct: 151 NLDLHL-HKGYTITRYLYATSNINLPNFFQKSKLSSIW-SQHANWMGFVAVATDEEEVSR 210

Query: 259 MGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDP---KVECGFLSLYKTA 318
           +GRRDIVIA RGT T LEW  +++         D   + + GD    K+E GF  LY   
Sbjct: 211 LGRRDIVIAWRGTVTYLEWIYDLK---------DILCSANFGDDPSIKIELGFHDLYTKK 270

Query: 319 GAHVK----SLSESVVEEIRRLTEIY----KGETLSITVTGHSLGAALALLVADEIS-VC 378
               K    S  E V+ E++RL E Y    +G   SITVTGHSLGA+LAL+ A +I+ + 
Sbjct: 271 EDSCKFSSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELN 330

Query: 379 APEVP------PVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP------- 438
              VP      P+ VFSF GPRVGN  F +R     VKVLR+VN  D +  VP       
Sbjct: 331 LNHVPENNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEK 390

Query: 439 -------------PVTYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGF 449
                        P +Y+HVG EL ++ K SP+LKP  D+ C H+LEA LHLVDG+
Sbjct: 391 FQFQKYVEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGY 435

BLAST of Spg038193 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 232.3 bits (591), Expect = 1.2e-59
Identity = 138/350 (39.43%), Postives = 197/350 (56.29%), Query Frame = 0

Query: 139 WREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSNPSTSP------NEAPLPR 198
           WR+  G +DW+GL+DP+D  LR E++RYGE  QA Y +F  +P++               
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 199 HVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVAVCDDRREIARMG 258
            + + D  Y+V + LYATS++ LP++         W ++ ++W+GYVAV DD     R+G
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVW-SKNANWMGYVAVSDDETSRNRLG 224

Query: 259 RRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDP--KVECGFLSLYKTAGAH 318
           RRDI IA RGT T LEW  +++ +L  +  +     + C DP  KVE GFL LY      
Sbjct: 225 RRDIAIAWRGTVTKLEWIADLKDYLKPVTEN----KIRCPDPAVKVESGFLDLYTDKDTT 284

Query: 319 VK----SLSESVVEEIRRLTEIYKGE---TLSITVTGHSLGAALALLVADEISVC----- 378
            K    S  E ++ E++RL E +  +    LSITVTGHSLG ALA+L A +I+       
Sbjct: 285 CKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRS 344

Query: 379 -APEVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP------------ 438
              +V PV V ++GGPRVGN  F +R+    VKV+R+VN  DV+ + P            
Sbjct: 345 KKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHAL 404

Query: 439 -------PVTYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGF 449
                  P  YSHVG EL ++ + SP+LKP+ DV+  H+LEA LHL+DG+
Sbjct: 405 MKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGY 449

BLAST of Spg038193 vs. ExPASy Swiss-Prot
Match: Q941F1 (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4 PE=1 SV=2)

HSP 1 Score: 231.9 bits (590), Expect = 1.6e-59
Identity = 142/351 (40.46%), Postives = 195/351 (55.56%), Query Frame = 0

Query: 139 WREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSNP------STSPNEAPLPR 198
           WR+  G +DW+GL+DP+D  LR E++RYGE  QA Y +F  +P      S       L  
Sbjct: 89  WRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLFD 148

Query: 199 HVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVAVCDDRREI-ARM 258
            + + D  Y+V + LYATS++ LP++         W ++ ++W+GYVAV DD      R+
Sbjct: 149 SLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVW-SKNANWMGYVAVSDDNEATRCRL 208

Query: 259 GRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDP--KVECGFLSLY--KTA 318
           GRRDI IA RGT T LEW  +++  L  +  +       C DP  K E GFL LY  K  
Sbjct: 209 GRRDIAIAWRGTVTRLEWIADLKDFLKPVSGN----GFRCPDPAVKAESGFLDLYTDKDT 268

Query: 319 GAHVKSLS--ESVVEEIRRLTEIY---KGETLSITVTGHSLGAALALLVADEISVCA--- 378
             +    S  E V+ E++RL E Y   +GE LSITVTGHSLG ALA+L A +++      
Sbjct: 269 SCNFSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNR 328

Query: 379 ---PEVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP----------- 438
               +V PV  F++GGPRVGN  F +RI    VKVLR+VN  DV+ + P           
Sbjct: 329 TRKGKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQA 388

Query: 439 --------PVTYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGF 449
                   P  YSHVG  L ++ + SP+LKP  D++  H+LEA LHL+DG+
Sbjct: 389 LMKLAGGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGY 434

BLAST of Spg038193 vs. ExPASy TrEMBL
Match: A0A5D3E612 (Phospholipase A1-Ibeta2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003630 PE=4 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 2.1e-256
Identity = 438/503 (87.08%), Postives = 471/503 (93.64%), Query Frame = 0

Query: 3   LISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFK--PMPVDGTRRHLANLDKLLK 62
           +I+ST+P QNLS + +RRS+FKYQ+SPLNPSA  ISSF+   + V  TRRHLANLDKLL 
Sbjct: 4   VINSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLN 63

Query: 63  KTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAAEEMSPRNLNRLQ 122
           KTDPP K++P+PVHKDPNKGS   MPI+GKGLLEGLNL+R+WPE+KA EEMSPRNLNRLQ
Sbjct: 64  KTDPPLKVDPQPVHKDPNKGS-LHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQ 123

Query: 123 RLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSN 182
           RLLSKTVEYSPRNALG+RWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYH+FHSN
Sbjct: 124 RLLSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSN 183

Query: 183 PSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVA 242
           P+TSPNE PLPRHV LPDRSYKVTKSLYATSSVGLP W+D+VAPDLGWMTQRSSWVGYVA
Sbjct: 184 PTTSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVA 243

Query: 243 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVECGF 302
           VCDDRREIARMGRRDIVIALRGTATCLEWAENVRA LIDIPAD      DCGDPKVECGF
Sbjct: 244 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPAD--VDTKDCGDPKVECGF 303

Query: 303 LSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISVCAP 362
           LSLYKTAGAHV+SLSESVVEEIRRLTE+YKGETLSITVTGHSLGAALA+LVADEISVC+ 
Sbjct: 304 LSLYKTAGAHVRSLSESVVEEIRRLTEMYKGETLSITVTGHSLGAALAILVADEISVCSA 363

Query: 363 EVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP--PVTYSHVGTELRV 422
           EVPPVAVFSFGGPRVGN++FADRI+SRNVKVLRIVNSQDVITQVP  P+TYSHVGTELRV
Sbjct: 364 EVPPVAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRV 423

Query: 423 ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQRGNVKKLY 482
           ETKMSP+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQRGN+KKLY
Sbjct: 424 ETKMSPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLY 483

Query: 483 MRKVKDLGFNPELQTAGCLPSPS 502
           MRKVKDLGFNPELQT GCLPSPS
Sbjct: 484 MRKVKDLGFNPELQTVGCLPSPS 503

BLAST of Spg038193 vs. ExPASy TrEMBL
Match: A0A1S3CPU9 (phospholipase A1-Ibeta2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503457 PE=4 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 2.1e-256
Identity = 438/503 (87.08%), Postives = 471/503 (93.64%), Query Frame = 0

Query: 3   LISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFK--PMPVDGTRRHLANLDKLLK 62
           +I+ST+P QNLS + +RRS+FKYQ+SPLNPSA  ISSF+   + V  TRRHLANLDKLL 
Sbjct: 4   VINSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLN 63

Query: 63  KTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAAEEMSPRNLNRLQ 122
           KTDPP K++P+PVHKDPNKGS   MPI+GKGLLEGLNL+R+WPE+KA EEMSPRNLNRLQ
Sbjct: 64  KTDPPLKVDPQPVHKDPNKGS-LHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQ 123

Query: 123 RLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSN 182
           RLLSKTVEYSPRNALG+RWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYH+FHSN
Sbjct: 124 RLLSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSN 183

Query: 183 PSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVA 242
           P+TSPNE PLPRHV LPDRSYKVTKSLYATSSVGLP W+D+VAPDLGWMTQRSSWVGYVA
Sbjct: 184 PTTSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVA 243

Query: 243 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVECGF 302
           VCDDRREIARMGRRDIVIALRGTATCLEWAENVRA LIDIPAD      DCGDPKVECGF
Sbjct: 244 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPAD--VDTKDCGDPKVECGF 303

Query: 303 LSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISVCAP 362
           LSLYKTAGAHV+SLSESVVEEIRRLTE+YKGETLSITVTGHSLGAALA+LVADEISVC+ 
Sbjct: 304 LSLYKTAGAHVRSLSESVVEEIRRLTEMYKGETLSITVTGHSLGAALAILVADEISVCSA 363

Query: 363 EVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP--PVTYSHVGTELRV 422
           EVPPVAVFSFGGPRVGN++FADRI+SRNVKVLRIVNSQDVITQVP  P+TYSHVGTELRV
Sbjct: 364 EVPPVAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRV 423

Query: 423 ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQRGNVKKLY 482
           ETKMSP+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQRGN+KKLY
Sbjct: 424 ETKMSPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLY 483

Query: 483 MRKVKDLGFNPELQTAGCLPSPS 502
           MRKVKDLGFNPELQT GCLPSPS
Sbjct: 484 MRKVKDLGFNPELQTVGCLPSPS 503

BLAST of Spg038193 vs. ExPASy TrEMBL
Match: A0A5A7T4U1 (Phospholipase A1-Ibeta2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00160 PE=4 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 6.2e-256
Identity = 437/503 (86.88%), Postives = 471/503 (93.64%), Query Frame = 0

Query: 3   LISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFK--PMPVDGTRRHLANLDKLLK 62
           +I+ST+P QNLS + +RRS+FKYQ+SPLNPSA  ISSF+   + V  TRRHLANLDKLL 
Sbjct: 4   VINSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLN 63

Query: 63  KTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAAEEMSPRNLNRLQ 122
           KTDPP K++P+PVHKDPNKGS   MPI+GKGLLEGLNL+R+WPE+KA EEMSPRNLNRLQ
Sbjct: 64  KTDPPLKVDPQPVHKDPNKGS-LHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQ 123

Query: 123 RLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSN 182
           RLLSKTVEYSPRNALG+RWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYH+FHSN
Sbjct: 124 RLLSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSN 183

Query: 183 PSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVA 242
           P+TSPNE PLPRHV LPDRSYKVTKSLYATSSVGLP W+D+VAPDLGWMTQRSSWVGYVA
Sbjct: 184 PTTSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVA 243

Query: 243 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVECGF 302
           VCDDRREIARMGRRDIVIALRGTATCLEWAENVRA LIDIPAD      DCGDPKVECGF
Sbjct: 244 VCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPAD--VDTKDCGDPKVECGF 303

Query: 303 LSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISVCAP 362
           LSLYKTAGAHV+SLSESVVEEIR+LTE+YKGETLSITVTGHSLGAALA+LVADEISVC+ 
Sbjct: 304 LSLYKTAGAHVRSLSESVVEEIRQLTEMYKGETLSITVTGHSLGAALAILVADEISVCSA 363

Query: 363 EVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP--PVTYSHVGTELRV 422
           EVPPVAVFSFGGPRVGN++FADRI+SRNVKVLRIVNSQDVITQVP  P+TYSHVGTELRV
Sbjct: 364 EVPPVAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRV 423

Query: 423 ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQRGNVKKLY 482
           ETKMSP+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQRGN+KKLY
Sbjct: 424 ETKMSPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLY 483

Query: 483 MRKVKDLGFNPELQTAGCLPSPS 502
           MRKVKDLGFNPELQT GCLPSPS
Sbjct: 484 MRKVKDLGFNPELQTVGCLPSPS 503

BLAST of Spg038193 vs. ExPASy TrEMBL
Match: A0A6J1EV72 (phospholipase A1-Ibeta2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111438059 PE=4 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.5e-254
Identity = 442/503 (87.87%), Postives = 465/503 (92.45%), Query Frame = 0

Query: 1   MQLISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFKP--MPVDGTRRHLANLDKL 60
           MQ+I+ST+PAQNLS ++VRRS+FKYQMSPLNPSAA IS F+P    VDGTRRHLANLDKL
Sbjct: 1   MQIINSTIPAQNLSRFQVRRSSFKYQMSPLNPSAAKISYFRPATAAVDGTRRHLANLDKL 60

Query: 61  LKKTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAAEEMSPRNLNR 120
           LKKTD PAK+EPEPV KDPNK  GS MPI+GKGLLEGLNLARIWPEM+AAE+MSPRNLNR
Sbjct: 61  LKKTDTPAKVEPEPVFKDPNK--GSVMPIKGKGLLEGLNLARIWPEMRAAEDMSPRNLNR 120

Query: 121 LQRLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFH 180
           LQRLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQ+AYHSFH
Sbjct: 121 LQRLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQSAYHSFH 180

Query: 181 SNPSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGY 240
           SNP+ S NEAPLPRHVALPD+SYKVTKSLYATSSVGLP WVD+VAPDLGWMTQRSSW+GY
Sbjct: 181 SNPAMSLNEAPLPRHVALPDKSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWMGY 240

Query: 241 VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVEC 300
           VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRA L +IP D      +  DPKVEC
Sbjct: 241 VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLTNIPTD-----TNSSDPKVEC 300

Query: 301 GFLSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISVC 360
           GFLSLYKTAGAHVKSLSESVVEEIRRL EIYKGETLSI+VTGHSLGAALALLVADEISVC
Sbjct: 301 GFLSLYKTAGAHVKSLSESVVEEIRRLMEIYKGETLSISVTGHSLGAALALLVADEISVC 360

Query: 361 APEVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVPPVTYSHVGTELRV 420
           +P VPPVAVFSFGGPRVGN  FA+RI SRNVKVLRIVNSQD+ITQVPPVTYSHVGTELRV
Sbjct: 361 SPTVPPVAVFSFGGPRVGNTAFANRITSRNVKVLRIVNSQDLITQVPPVTYSHVGTELRV 420

Query: 421 ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQRGNVKKLY 480
           ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFR NAKRSL RLV DQRGN+KKLY
Sbjct: 421 ETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRANAKRSLVRLVHDQRGNMKKLY 480

Query: 481 MRKVKDLGFNPELQTAGCLPSPS 502
           MRKVKDL  NPELQ  GCLPSPS
Sbjct: 481 MRKVKDLSLNPELQMLGCLPSPS 496

BLAST of Spg038193 vs. ExPASy TrEMBL
Match: A0A0A0LEL7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G914060 PE=4 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.4e-254
Identity = 436/505 (86.34%), Postives = 471/505 (93.27%), Query Frame = 0

Query: 1   MQLISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFK--PMPVDGTRRHLANLDKL 60
           MQ+I+ST+P QNLS + +RRS+FKYQ+SPLNPSA  I+SF+   + V  TRRHLANLDKL
Sbjct: 1   MQVINSTIPPQNLSQFHLRRSSFKYQISPLNPSAPKITSFRSAAVAVHVTRRHLANLDKL 60

Query: 61  LKKTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAAEEMSPRNLNR 120
           L KTDPP K++P+PVHKDPNK   S MPI+GKGLLE LNLAR+WPE+KAAE+MSPRNLNR
Sbjct: 61  LNKTDPPLKVDPQPVHKDPNK--VSPMPIKGKGLLESLNLARLWPEVKAAEDMSPRNLNR 120

Query: 121 LQRLLSKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFH 180
           LQRLLSKTVEYSPRNALG+RWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYH+FH
Sbjct: 121 LQRLLSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFH 180

Query: 181 SNPSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGY 240
           SNP+TSPNE PLPRHVALPDRSYKVTKSLYATSSVGLP W+D+VAPDLGWMTQRSSWVGY
Sbjct: 181 SNPTTSPNEPPLPRHVALPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGY 240

Query: 241 VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVEC 300
           VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRA L ++PAD      D GDPKVEC
Sbjct: 241 VAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAQLTNVPAD--VDTKDGGDPKVEC 300

Query: 301 GFLSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISVC 360
           GFLSLYKTAGAHVKSLSESVVEEIRRLTE+YKGETLSITVTGHSLGAALA+LVADEISVC
Sbjct: 301 GFLSLYKTAGAHVKSLSESVVEEIRRLTELYKGETLSITVTGHSLGAALAILVADEISVC 360

Query: 361 APEVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP--PVTYSHVGTEL 420
           + EVPPVAVFSFGGPRVGN+IFADRI+SRNVKVLRIVNSQD+ITQVP  P+TYSHVGTEL
Sbjct: 361 SAEVPPVAVFSFGGPRVGNKIFADRIKSRNVKVLRIVNSQDLITQVPPNPMTYSHVGTEL 420

Query: 421 RVETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQRGNVKK 480
           RVETKMSP+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQRGN+KK
Sbjct: 421 RVETKMSPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKK 480

Query: 481 LYMRKVKDLGFNPELQTAGCLPSPS 502
           LYMRKVKDLGFNPELQT GCLPSPS
Sbjct: 481 LYMRKVKDLGFNPELQTVGCLPSPS 501

BLAST of Spg038193 vs. TAIR 10
Match: AT4G16820.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 551.6 bits (1420), Expect = 6.5e-157
Identity = 305/535 (57.01%), Postives = 363/535 (67.85%), Query Frame = 0

Query: 4   ISSTVPAQNLSGYRVRRSTFKYQMSPLNPSAANISSFKPMPVDGTRR----HLANLDKLL 63
           + +  P  ++   + RR T     S L P+       KP+ V   R+    HL NL+ +L
Sbjct: 1   MQTLTPNADIFHAKRRRFTCNTHTSTLIPT-------KPLSVSPARKTNKEHLRNLENVL 60

Query: 64  KKTDPPAKLEPEPVHKDPNKGSGSSMPIRGKGLLEGLNLARIWPEMKAA-EEMSPRNLNR 123
           + +        + +    ++   ++       LL GLNLARIWP+MKAA +EMSP+NL R
Sbjct: 61  RTSSNSI----DHIENVTSRQEKTTKNTSTSSLLGGLNLARIWPQMKAAVDEMSPKNLKR 120

Query: 124 LQRLLSK-TVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSF 183
           LQRLLSK + E SP++ LG +WRE HG N+W+GLLDPLDENLRRE+VRYGEFVQAAYH+F
Sbjct: 121 LQRLLSKSSEERSPKSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAF 180

Query: 184 HSNPSTSPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVG 243
           HS+P  S      PRHVALPD S+KVTKSLYATSSV LP W+DDVAPDL WMT+++SWVG
Sbjct: 181 HSDPEGS------PRHVALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVG 240

Query: 244 YVAVCDDRREIARMGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDPKVE 303
           YVAVCDD REI RMGRR+IVIALRGTAT LEW+EN R +L+ +P +      D   PKVE
Sbjct: 241 YVAVCDDPREIRRMGRREIVIALRGTATLLEWSENFRPNLVSMP-EPKPDQSDPTRPKVE 300

Query: 304 CGFLSLYKTAGAHVKSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISV 363
           CGF SLY T   H  SL+ES+V EI RL E+Y GE LSI+VTGHSLGAA+ALL AD+I+ 
Sbjct: 301 CGFNSLYTTGDQHAPSLAESLVGEISRLVELYAGEELSISVTGHSLGAAIALLAADDIAE 360

Query: 364 CAPEVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP------------ 423
             P  PPVAVFSFGGPRVGNR FADR+ S+ VKVLR+VNSQDV+T+VP            
Sbjct: 361 RVPHAPPVAVFSFGGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQS 420

Query: 424 ------------------PVTYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDG 483
                             P  YSHVG ELRV+ KMSPYLKPNADVACCHDLEAYLHLVDG
Sbjct: 421 RNNGRSPGGIMEMVERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDG 480

Query: 484 FLSSKCPFRPNAKRSLARLVQDQRGNVKKLYMRKVKDLGFNPELQTAG-CLPSPS 502
           FL+S CPFR NAKRSL +L+ +QR NVK LY    K L  N      G  LPSPS
Sbjct: 481 FLASNCPFRANAKRSLRKLLDEQRSNVKVLYTG--KSLRLNRTFHDNGDVLPSPS 515

BLAST of Spg038193 vs. TAIR 10
Match: AT2G44810.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 321.6 bits (823), Expect = 1.1e-87
Identity = 177/364 (48.63%), Postives = 228/364 (62.64%), Query Frame = 0

Query: 141 EYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSNPSTS-------PNEAPLPRH 200
           EY G  +W GLLDPLD+NLRRE++RYG+FV++AY +F  +PS+        P    L R 
Sbjct: 2   EYQGLQNWDGLLDPLDDNLRREILRYGQFVESAYQAFDFDPSSPTYGTCRFPRSTLLERS 61

Query: 201 VALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVAVCDDRREIARMGR 260
             LP+  Y++TK+L ATS + LP W++  AP   WM  +SSW+GYVAVC D+ EI+R+GR
Sbjct: 62  -GLPNSGYRLTKNLRATSGINLPRWIEK-AP--SWMATQSSWIGYVAVCQDKEEISRLGR 121

Query: 261 RDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGD--PKVECGFLSLYKTAGAHV 320
           RD+VI+ RGTATCLEW EN+RA L  +P     A ++  +  P VE GFLSLY T+G H 
Sbjct: 122 RDVVISFRGTATCLEWLENLRATLTHLPNGPTGANLNGSNSGPMVESGFLSLY-TSGVH- 181

Query: 321 KSLSESVVEEIRRLTEIYKGETLSITVTGHSLGAALALLVADEISVCAPEVPPVAVFSFG 380
            SL + V EEI RL + Y  E LS+T+TGHSLGAA+A L A +I       P V V SFG
Sbjct: 182 -SLRDMVREEIARLLQSYGDEPLSVTITGHSLGAAIATLAAYDIKTTFKRAPMVTVISFG 241

Query: 381 GPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP-------------------------- 440
           GPRVGNR F   +  +  KVLRIVNS DVIT+VP                          
Sbjct: 242 GPRVGNRCFRKLLEKQGTKVLRIVNSDDVITKVPGVVLENREQDNVKMTASIMPSWIQRR 301

Query: 441 ----PVTYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKR 466
               P  Y+ +G ELR+ ++ SP+L  + +VA CH+L+ YLHLVDGF+SS CPFR  A+R
Sbjct: 302 VEETPWVYAEIGKELRLSSRDSPHLS-SINVATCHELKTYLHLVDGFVSSTCPFRETARR 357

BLAST of Spg038193 vs. TAIR 10
Match: AT1G30370.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 259.6 bits (662), Expect = 5.1e-69
Identity = 152/368 (41.30%), Postives = 213/368 (57.88%), Query Frame = 0

Query: 130 SPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSNP------ST 189
           SPR  + + WRE HG N+W  LLDPL   LRREV +YGEFV++ Y S   +P      S+
Sbjct: 109 SPREKISKMWRELHGSNNWENLLDPLHPWLRREVTKYGEFVESVYDSLDFDPLSEFCGSS 168

Query: 190 SPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVAVCD 249
             N   L   + L    YKVTK +YA S V +P W    A    W ++ S+W+G+VAV  
Sbjct: 169 RYNRNKLFEELGLTRHGYKVTKYIYAMSRVDVPQWFLSSALGETW-SKDSNWMGFVAVSG 228

Query: 250 DRREIARMGRRDIVIALRGTATCLEWAENVRAHL--IDIPADDAAAAVDCGDPKVECGFL 309
           D RE  R+GRRDIV+A RGT T  EW  ++R  +   D         V     KV+ GFL
Sbjct: 229 D-RESLRIGRRDIVVAWRGTVTPTEWFMDLRTSMEPFDCEGKHGKTVV-----KVQSGFL 288

Query: 310 SLYKTAGA----HVKSLSESVVEEIRRLTEIYK--GETLSITVTGHSLGAALALLVADEI 369
           S+Y +       + +S SE  ++E++RL   +K  GE +S+T+TGHSLG ALAL+ A E 
Sbjct: 289 SIYNSKSELTRYNKESASEQTMDEVKRLVNFFKDRGEEVSLTITGHSLGGALALMNAYEA 348

Query: 370 SVCAPEVP-PVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP--------- 429
           +   P +   ++V SFG PRVGN  F +++ S  VKVLR+VN QD++ ++P         
Sbjct: 349 ARDVPALSGNISVISFGAPRVGNLAFKEKLNSLGVKVLRVVNKQDIVPKLPGIVFNKVLN 408

Query: 430 ---PVT------YSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFR 465
              P+T      Y HVGT+L+++   SPY+K ++D+   H+LE YLH++DGF   K  FR
Sbjct: 409 KLNPITSRLNWVYRHVGTQLKLDVFSSPYVKRDSDLGRAHNLEVYLHVLDGFHRKKSGFR 468

BLAST of Spg038193 vs. TAIR 10
Match: AT1G51440.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 2.0e-65
Identity = 148/356 (41.57%), Postives = 201/356 (56.46%), Query Frame = 0

Query: 139 WREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSNPSTS--------PNEAPL 198
           WRE  GCN+W G LDP++ +LRRE++RYGEF QA Y SF  +P +         P++  L
Sbjct: 91  WREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPSDFFL 150

Query: 199 PRHVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVAVCDDRREIAR 258
              + L  + Y +T+ LYATS++ LP++         W +Q ++W+G+VAV  D  E++R
Sbjct: 151 NLDLHL-HKGYTITRYLYATSNINLPNFFQKSKLSSIW-SQHANWMGFVAVATDEEEVSR 210

Query: 259 MGRRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDP---KVECGFLSLYKTA 318
           +GRRDIVIA RGT T LEW  +++         D   + + GD    K+E GF  LY   
Sbjct: 211 LGRRDIVIAWRGTVTYLEWIYDLK---------DILCSANFGDDPSIKIELGFHDLYTKK 270

Query: 319 GAHVK----SLSESVVEEIRRLTEIY----KGETLSITVTGHSLGAALALLVADEIS-VC 378
               K    S  E V+ E++RL E Y    +G   SITVTGHSLGA+LAL+ A +I+ + 
Sbjct: 271 EDSCKFSSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELN 330

Query: 379 APEVP------PVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP------- 438
              VP      P+ VFSF GPRVGN  F +R     VKVLR+VN  D +  VP       
Sbjct: 331 LNHVPENNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEK 390

Query: 439 -------------PVTYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGF 449
                        P +Y+HVG EL ++ K SP+LKP  D+ C H+LEA LHLVDG+
Sbjct: 391 FQFQKYVEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGY 435

BLAST of Spg038193 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 234.2 bits (596), Expect = 2.3e-61
Identity = 139/352 (39.49%), Postives = 199/352 (56.53%), Query Frame = 0

Query: 139 WREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHSFHSNPSTSP------NEAPLPR 198
           WR+  G +DW+GL+DP+D  LR E++RYGE  QA Y +F  +P++               
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 199 HVALPDRSYKVTKSLYATSSVGLPSWVDDVAPDLGWMTQRSSWVGYVAVCDDRREIARMG 258
            + + D  Y+V + LYATS++ LP++         W ++ ++W+GYVAV DD     R+G
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVW-SKNANWMGYVAVSDDETSRNRLG 224

Query: 259 RRDIVIALRGTATCLEWAENVRAHLIDIPADDAAAAVDCGDP--KVECGFLSLYKTAGAH 318
           RRDI IA RGT T LEW  +++ +L  +  +     + C DP  KVE GFL LY      
Sbjct: 225 RRDIAIAWRGTVTKLEWIADLKDYLKPVTEN----KIRCPDPAVKVESGFLDLYTDKDTT 284

Query: 319 VK----SLSESVVEEIRRLTEIYKGE---TLSITVTGHSLGAALALLVADEISVC----- 378
            K    S  E ++ E++RL E +  +    LSITVTGHSLG ALA+L A +I+       
Sbjct: 285 CKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRS 344

Query: 379 -APEVPPVAVFSFGGPRVGNRIFADRIRSRNVKVLRIVNSQDVITQVP------------ 438
              +V PV V ++GGPRVGN  F +R+    VKV+R+VN  DV+ + P            
Sbjct: 345 KKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHAL 404

Query: 439 -------PVTYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGFLS 451
                  P  YSHVG EL ++ + SP+LKP+ DV+  H+LEA LHL+DG++S
Sbjct: 405 MKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYVS 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887009.12.3e-26891.65phospholipase A1-Ibeta2, chloroplastic [Benincasa hispida][more]
XP_008465868.24.4e-25687.08PREDICTED: phospholipase A1-Ibeta2, chloroplastic [Cucumis melo] >TYK31086.1 pho... [more]
XP_023554175.17.5e-25688.47phospholipase A1-Ibeta2, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAA0038492.11.3e-25586.88phospholipase A1-Ibeta2 [Cucumis melo var. makuwa][more]
XP_022931784.13.2e-25487.87phospholipase A1-Ibeta2, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O235229.2e-15657.01Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16... [more]
Q948R12.3e-9048.43Phospholipase A(1) DAD1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAD1 P... [more]
Q9C8J62.8e-6441.57Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2... [more]
Q3EBR61.2e-5939.43Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3... [more]
Q941F11.6e-5940.46Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4... [more]
Match NameE-valueIdentityDescription
A0A5D3E6122.1e-25687.08Phospholipase A1-Ibeta2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3CPU92.1e-25687.08phospholipase A1-Ibeta2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503457 P... [more]
A0A5A7T4U16.2e-25686.88Phospholipase A1-Ibeta2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1EV721.5e-25487.87phospholipase A1-Ibeta2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A0A0LEL73.4e-25486.34Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G914060 PE... [more]
Match NameE-valueIdentityDescription
AT4G16820.16.5e-15757.01alpha/beta-Hydrolases superfamily protein [more]
AT2G44810.11.1e-8748.63alpha/beta-Hydrolases superfamily protein [more]
AT1G30370.15.1e-6941.30alpha/beta-Hydrolases superfamily protein [more]
AT1G51440.12.0e-6541.57alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.12.3e-6139.49alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 138..484
e-value: 6.4E-102
score: 343.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 159..448
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 257..407
e-value: 2.9E-33
score: 114.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..76
NoneNo IPR availablePANTHERPTHR31403:SF37GLYCEROLIPASE A1coord: 1..406
NoneNo IPR availablePANTHERPTHR31403:SF37GLYCEROLIPASE A1coord: 406..488
NoneNo IPR availablePANTHERPTHR31403PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTICcoord: 1..406
coord: 406..488
NoneNo IPR availableCDDcd00519Lipase_3coord: 190..446
e-value: 5.17176E-31
score: 117.963

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg038193.1Spg038193.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0004620 phospholipase activity