Spg038190 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg038190
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRibosome-recycling factor, chloroplastic
Locationscaffold12: 43204793 .. 43206478 (+)
RNA-Seq ExpressionSpg038190
SyntenySpg038190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACGCCTTTCTCCTCCACCACTTCTGTCCGTTCCGTCTTCCAACCCAAACAAAAACCCCCTCCCAAAGCCCTCCTCTCGTTCCGAGTAGATTATAATGGATGGTTAAATTACGCAAAATGCGGTTCGAGTACGGTGCATCTATGTTCATGGAAGTCAGCTTCGAACTTCCTTAGCCTCAAGCGGTATGCCCTAGTGCTCTCTACCCAACTCTTTGTTTCTGGGAATCTGTTGAAAAACAGGCTAAGCTATTTCTTTCATTTCATTTACTTTCTTACTGTTCTTGTCGCGCTGTGCCATGTGAATTTGTGTAAATTGTACACCCTCTGAGAAGTGGTAATATGGAACAAAAGTATGATAAACATACAAGCATTTTGCATTTGGATTCGTCATTGCCTTATTGCTCCGTGAAGTTTACTGTGGTGGCTTTTCAGTTGTGTTGACATTCTTCTCCCCCTCCTTTTTTTTGGCCATGACAATTTTCAGAAGAGGAGTCGTGAGAGCTGCAACTATCGAAGAAATTGAAGCGGAGAAGACATTGATTGAGAAAGATGTAGTAAGTTCATATGTTCATTGATTTGGAGACTTGCTGTGTTTTTTAATCAAGTAATCTAAGGTTAGGAACATTGTTGCGAATTACCATTGAATTACTTTCCAGAGAGCAAGGATGGAAAAGACTATAGATACTGTTCGGTCTAGTTTCAACTCAATAAGGACTGGCAGAGCAAATCCTGCCATGCTTGATAAGATTGAGGTATATGCTAATTCTTAAACTCAGGTCGTTGGTGGATAGATGTCTACTTAGAACATTTATTTTTTCAAACTATCAGATGAGTATCATGCCAATCATATCACTACCATACAAAAATTTGTCGCTTTTTCATGATTTTCCAGGTGGAGTATTACGGCAGTCCAGTTAGCCTGAAGAGCATTGCCCAAATTAGTACACCTGATGCAAGCTCTCTCTTGGTCCAACCATATGATAAATCCAGGTACTCCATGTGCCAGTTGGCTGAGTTGAATATATTTGGAAACCTTTTGGGATATGCTGGTCATTTGGTGTACCTTTTCCTTATAGAAAACAAGCACTTTACGGATTAAGCTTTTCCAAATTCCTGTTATTTTAGAATCATAAAGGGGTAAATATAAAACAAAAGGACAAGTTTTCTTCGATCACCATACCTTTTACTCTAAACCCTTGAATATCGAGGATCTTCGTCAATCCTTTATTCATTTGAAACCTGTAGTTACTAGTCTTAGCTTGCTTTTCTTACCTACTTAATGCTTCAGAATTTTGTCATTGTAATTCCTGTTTTTCCGAGAAAAAGGTTTCAGACGTGCAGTTGGAGCTTTTAGATCTTCTCTAATCTTGAGTCTGTGACTTCATTGGTTGAAACTGCTATGGACATAACCATCCTATTATTATTAGCTATAGAATGATGTCATCCAGCTGTACAGTGTAATGCATTTGTATAATGTCTCTTGGCAATCAGCATTAACTCTTAACGAGTCATCATCTTCAGCTTGAAGTCCATAGAGAAGGCCATAGTCAACTCTGACTTGGGATTGACTCCCAACAATGATGGAGAAGTCATACGGTTGACTCTCCCTCAACTCACATCCGAAAGAAGAAAGGTATTTTCCTTTGCGTTTGCTAACATTTTTGAACAAGATGTAGCATAA

mRNA sequence

ATGGCGACGCCTTTCTCCTCCACCACTTCTGTCCGTTCCGTCTTCCAACCCAAACAAAAACCCCCTCCCAAAGCCCTCCTCTCGTTCCGAGTAGATTATAATGGATGGTTAAATTACGCAAAATGCGGTTCGAGTACGGTGCATCTATGTTCATGGAAGTCAGCTTCGAACTTCCTTAGCCTCAAGCGGTATGCCCTAGTGCTCTCTACCCAACTCTTTGTTTCTGGGAATCTGTTGAAAAACAGGCTAAGCTATTTCTTTCATTTCATTTACTTTCTTACTGTTCTTGTCGCGCTGTGCCATCATTTTGCATTTGGATTCGTCATTGCCTTATTGCTCCGTGAAGTTTACTGTGGTGGCTTTTCAGTTGTGTTGACATTCTTCTCCCCCTCCTTTTTTTTGGCCATGACAATTTTCAGAAGAGGAGTCGTGAGAGCTGCAACTATCGAAGAAATTGAAGCGGAGAAGACATTGATTGAGAAAGATGTAAGAGCAAGGATGGAAAAGACTATAGATACTGTTCGGTCTAGTTTCAACTCAATAAGGACTGGCAGAGCAAATCCTGCCATGCTTGATAAGATTGAGGTGGAGTATTACGGCAGTCCAGTTAGCCTGAAGAGCATTGCCCAAATTAGTACACCTGATGCAAGCTCTCTCTTGGTCCAACCATATGATAAATCCAGCTTGAAGTCCATAGAGAAGGCCATAGTCAACTCTGACTTGGGATTGACTCCCAACAATGATGGAGAAGTCATACGGTTGACTCTCCCTCAACTCACATCCGAAAGAAGAAAGGTATTTTCCTTTGCGTTTGCTAACATTTTTGAACAAGATGTAGCATAA

Coding sequence (CDS)

ATGGCGACGCCTTTCTCCTCCACCACTTCTGTCCGTTCCGTCTTCCAACCCAAACAAAAACCCCCTCCCAAAGCCCTCCTCTCGTTCCGAGTAGATTATAATGGATGGTTAAATTACGCAAAATGCGGTTCGAGTACGGTGCATCTATGTTCATGGAAGTCAGCTTCGAACTTCCTTAGCCTCAAGCGGTATGCCCTAGTGCTCTCTACCCAACTCTTTGTTTCTGGGAATCTGTTGAAAAACAGGCTAAGCTATTTCTTTCATTTCATTTACTTTCTTACTGTTCTTGTCGCGCTGTGCCATCATTTTGCATTTGGATTCGTCATTGCCTTATTGCTCCGTGAAGTTTACTGTGGTGGCTTTTCAGTTGTGTTGACATTCTTCTCCCCCTCCTTTTTTTTGGCCATGACAATTTTCAGAAGAGGAGTCGTGAGAGCTGCAACTATCGAAGAAATTGAAGCGGAGAAGACATTGATTGAGAAAGATGTAAGAGCAAGGATGGAAAAGACTATAGATACTGTTCGGTCTAGTTTCAACTCAATAAGGACTGGCAGAGCAAATCCTGCCATGCTTGATAAGATTGAGGTGGAGTATTACGGCAGTCCAGTTAGCCTGAAGAGCATTGCCCAAATTAGTACACCTGATGCAAGCTCTCTCTTGGTCCAACCATATGATAAATCCAGCTTGAAGTCCATAGAGAAGGCCATAGTCAACTCTGACTTGGGATTGACTCCCAACAATGATGGAGAAGTCATACGGTTGACTCTCCCTCAACTCACATCCGAAAGAAGAAAGGTATTTTCCTTTGCGTTTGCTAACATTTTTGAACAAGATGTAGCATAA

Protein sequence

MATPFSSTTSVRSVFQPKQKPPPKALLSFRVDYNGWLNYAKCGSSTVHLCSWKSASNFLSLKRYALVLSTQLFVSGNLLKNRLSYFFHFIYFLTVLVALCHHFAFGFVIALLLREVYCGGFSVVLTFFSPSFFLAMTIFRRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNSIRTGRANPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSDLGLTPNNDGEVIRLTLPQLTSERRKVFSFAFANIFEQDVA
Homology
BLAST of Spg038190 vs. NCBI nr
Match: XP_038888223.1 (ribosome-recycling factor, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 322.8 bits (826), Expect = 3.0e-84
Identity = 191/278 (68.71%), Postives = 197/278 (70.86%), Query Frame = 0

Query: 1   MATPFSSTTSV-RSVFQPKQKPPPKALLSFRVDYNGWLNYAKCGSSTVHLCSWKSASNFL 60
           MATPFSSTTSV RSVFQ  Q PPPKALLS R DYN  LN  KCGSST HLCSWKSASN++
Sbjct: 1   MATPFSSTTSVRRSVFQLTQNPPPKALLSLR-DYNAGLNCVKCGSSTTHLCSWKSASNYV 60

Query: 61  SLKRYALVLSTQLFVSGNLLKNRLSYFFHFIYFLTVLVALCHHFAFGFVIALLLREVYCG 120
            L R ALVLSTQL VSGNLLKN                                      
Sbjct: 61  RLHRQALVLSTQLSVSGNLLKN-------------------------------------- 120

Query: 121 GFSVVLTFFSPSFFLAMTIFRRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFN 180
                               RRGVVRAATIEEIEAEKTLIEKDV+ARMEKTIDTVRS+FN
Sbjct: 121 --------------------RRGVVRAATIEEIEAEKTLIEKDVKARMEKTIDTVRSNFN 180

Query: 181 SIRTGRANPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNS 240
           SIRTGR+NPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNS
Sbjct: 181 SIRTGRSNPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNS 219

Query: 241 DLGLTPNNDGEVIRLTLPQLTSERRKVFSFAFANIFEQ 278
           DLGLTPNNDGEVIRLTLPQLTSERRK  S   A   E+
Sbjct: 241 DLGLTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEE 219

BLAST of Spg038190 vs. NCBI nr
Match: XP_038888224.1 (ribosome-recycling factor, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 320.1 bits (819), Expect = 1.9e-83
Identity = 190/278 (68.35%), Postives = 196/278 (70.50%), Query Frame = 0

Query: 1   MATPFSSTTSV-RSVFQPKQKPPPKALLSFRVDYNGWLNYAKCGSSTVHLCSWKSASNFL 60
           MATPFSSTTSV RSVFQ  Q PPPKALLS R DYN  LN  KCGSST HLCSWKSASN++
Sbjct: 1   MATPFSSTTSVRRSVFQLTQNPPPKALLSLR-DYNAGLNCVKCGSSTTHLCSWKSASNYV 60

Query: 61  SLKRYALVLSTQLFVSGNLLKNRLSYFFHFIYFLTVLVALCHHFAFGFVIALLLREVYCG 120
            L R ALVLSTQL VSGNLLKN                                      
Sbjct: 61  RLHRQALVLSTQLSVSGNLLKN-------------------------------------- 120

Query: 121 GFSVVLTFFSPSFFLAMTIFRRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFN 180
                                RGVVRAATIEEIEAEKTLIEKDV+ARMEKTIDTVRS+FN
Sbjct: 121 ---------------------RGVVRAATIEEIEAEKTLIEKDVKARMEKTIDTVRSNFN 180

Query: 181 SIRTGRANPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNS 240
           SIRTGR+NPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNS
Sbjct: 181 SIRTGRSNPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNS 218

Query: 241 DLGLTPNNDGEVIRLTLPQLTSERRKVFSFAFANIFEQ 278
           DLGLTPNNDGEVIRLTLPQLTSERRK  S   A   E+
Sbjct: 241 DLGLTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEE 218

BLAST of Spg038190 vs. NCBI nr
Match: XP_023533411.1 (ribosome-recycling factor, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 315.5 bits (807), Expect = 4.7e-82
Identity = 181/277 (65.34%), Postives = 195/277 (70.40%), Query Frame = 0

Query: 1   MATPFSSTTSVRSVFQPKQKPPPKALLSFRVDYNGWLNYAKCGSSTVHLCSWKSASNFLS 60
           MATPFSSTTS+RSVFQPKQ PPPKALLS R DYN  LN  KCGS+T HL SWKSASN++ 
Sbjct: 1   MATPFSSTTSLRSVFQPKQIPPPKALLSIR-DYNAGLNCVKCGSTTTHLSSWKSASNYIR 60

Query: 61  LKRYALVLSTQLFVSGNLLKNRLSYFFHFIYFLTVLVALCHHFAFGFVIALLLREVYCGG 120
           ++R+AL+L TQ F SGNL K                                        
Sbjct: 61  IRRHALMLPTQPFQSGNLSK---------------------------------------- 120

Query: 121 FSVVLTFFSPSFFLAMTIFRRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNS 180
                             +RRGV+RAATIEEIEAEK LIEKDVRARMEKTIDTVRSSFNS
Sbjct: 121 ------------------YRRGVIRAATIEEIEAEKALIEKDVRARMEKTIDTVRSSFNS 180

Query: 181 IRTGRANPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSD 240
           IRTGRANPAMLDKIEVEYYGSPV LKSIAQISTPDASSLL+QPYDKSSLKSIEKAIVNS+
Sbjct: 181 IRTGRANPAMLDKIEVEYYGSPVGLKSIAQISTPDASSLLIQPYDKSSLKSIEKAIVNSN 218

Query: 241 LGLTPNNDGEVIRLTLPQLTSERRKVFSFAFANIFEQ 278
           LG+TPNNDGEVIRLTLPQLTSERRK  S   A   E+
Sbjct: 241 LGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEE 218

BLAST of Spg038190 vs. NCBI nr
Match: XP_022995869.1 (ribosome-recycling factor, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 309.7 bits (792), Expect = 2.6e-80
Identity = 178/277 (64.26%), Postives = 193/277 (69.68%), Query Frame = 0

Query: 1   MATPFSSTTSVRSVFQPKQKPPPKALLSFRVDYNGWLNYAKCGSSTVHLCSWKSASNFLS 60
           MATPFSSTTS+R VFQPKQ PPPKALLS R DYN  LN  KCGS+T+HL SWKSASN+  
Sbjct: 1   MATPFSSTTSLRPVFQPKQIPPPKALLSIR-DYNAGLNCVKCGSTTMHLSSWKSASNYFR 60

Query: 61  LKRYALVLSTQLFVSGNLLKNRLSYFFHFIYFLTVLVALCHHFAFGFVIALLLREVYCGG 120
           ++R+AL+L TQ F SGNL K                                        
Sbjct: 61  IRRHALMLPTQPFPSGNLSK---------------------------------------- 120

Query: 121 FSVVLTFFSPSFFLAMTIFRRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNS 180
                             +RRGV+RAATIEEIEAEK LIEKDVRARMEKTIDTVRSSFNS
Sbjct: 121 ------------------YRRGVIRAATIEEIEAEKALIEKDVRARMEKTIDTVRSSFNS 180

Query: 181 IRTGRANPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSD 240
           IRTGRANPAMLDKIEVEYYGSPV LKSIAQISTPDASSLL+QPYDKSSLK IEKAIVNS+
Sbjct: 181 IRTGRANPAMLDKIEVEYYGSPVGLKSIAQISTPDASSLLIQPYDKSSLKLIEKAIVNSN 218

Query: 241 LGLTPNNDGEVIRLTLPQLTSERRKVFSFAFANIFEQ 278
           LG+TPNNDG+VIRLTLPQLTSERRK  S   A   E+
Sbjct: 241 LGMTPNNDGDVIRLTLPQLTSERRKELSKVVAKQAEE 218

BLAST of Spg038190 vs. NCBI nr
Match: XP_022931084.1 (ribosome-recycling factor, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 309.7 bits (792), Expect = 2.6e-80
Identity = 179/277 (64.62%), Postives = 194/277 (70.04%), Query Frame = 0

Query: 1   MATPFSSTTSVRSVFQPKQKPPPKALLSFRVDYNGWLNYAKCGSSTVHLCSWKSASNFLS 60
           MATPFSSTTS+RSVF+PKQ PPPKALLS R DYN  LN  KCGS+T  L SWKSASN++ 
Sbjct: 1   MATPFSSTTSLRSVFRPKQIPPPKALLSIR-DYNAGLNCIKCGSTTTPLSSWKSASNYIR 60

Query: 61  LKRYALVLSTQLFVSGNLLKNRLSYFFHFIYFLTVLVALCHHFAFGFVIALLLREVYCGG 120
           ++R+AL+L TQ F SGNL K                                        
Sbjct: 61  IRRHALMLPTQPFQSGNLSK---------------------------------------- 120

Query: 121 FSVVLTFFSPSFFLAMTIFRRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNS 180
                             +RRGV+RAATIEEIEAEK LIEKDVRARMEKTIDTVRSSFNS
Sbjct: 121 ------------------YRRGVIRAATIEEIEAEKALIEKDVRARMEKTIDTVRSSFNS 180

Query: 181 IRTGRANPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSD 240
           IRTGRANPAMLDKIEVEYYGSPV LKSIAQISTPDASSLL+QPYDKSSLKSIEKAIVNS+
Sbjct: 181 IRTGRANPAMLDKIEVEYYGSPVGLKSIAQISTPDASSLLIQPYDKSSLKSIEKAIVNSN 218

Query: 241 LGLTPNNDGEVIRLTLPQLTSERRKVFSFAFANIFEQ 278
           LG+TPNNDGEVIRLTLPQLTSERRK  S   A   E+
Sbjct: 241 LGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEE 218

BLAST of Spg038190 vs. ExPASy Swiss-Prot
Match: Q9M1X0 (Ribosome-recycling factor, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RRF PE=2 SV=2)

HSP 1 Score: 204.5 bits (519), Expect = 1.5e-51
Identity = 106/138 (76.81%), Postives = 124/138 (89.86%), Query Frame = 0

Query: 140 RRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNSIRTGRANPAMLDKIEVEYY 199
           +R V+R+ATIEEIEAEK+ IE DV+++MEKTI+T+R+SFNSIRTGR+N AMLDKIEVEYY
Sbjct: 76  KRLVIRSATIEEIEAEKSAIETDVKSKMEKTIETLRTSFNSIRTGRSNAAMLDKIEVEYY 135

Query: 200 GSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSDLGLTPNNDGEVIRLTLPQL 259
           GSPVSLKSIAQISTPD SSLL+QPYDKSSLK+IEKAIVNSDLG+TPNNDG+VIRL+LP L
Sbjct: 136 GSPVSLKSIAQISTPDGSSLLLQPYDKSSLKAIEKAIVNSDLGVTPNNDGDVIRLSLPPL 195

Query: 260 TSERRKVFSFAFANIFEQ 278
           TS+RRK  S   A   E+
Sbjct: 196 TSDRRKELSKVVAKQSEE 213

BLAST of Spg038190 vs. ExPASy Swiss-Prot
Match: P37706 (Ribosome-recycling factor, chloroplastic (Fragment) OS=Daucus carota OX=4039 GN=RRF PE=2 SV=2)

HSP 1 Score: 196.1 bits (497), Expect = 5.5e-49
Identity = 100/138 (72.46%), Postives = 119/138 (86.23%), Query Frame = 0

Query: 140 RRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNSIRTGRANPAMLDKIEVEYY 199
           R G ++ AT+EEIEAEK+LIEK V+ RMEKTI+ V++SFNSIRT R+NP MLDKI+VEYY
Sbjct: 28  RTGTLKCATMEEIEAEKSLIEKSVKERMEKTIENVKASFNSIRTXRSNPDMLDKIKVEYY 87

Query: 200 GSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSDLGLTPNNDGEVIRLTLPQL 259
           G+P SLKSIAQISTPD+SSLLV PYDKSSLK IEKAIVNSDLG+TPNNDG+VIRL++PQL
Sbjct: 88  GTPTSLKSIAQISTPDSSSLLVNPYDKSSLKDIEKAIVNSDLGITPNNDGDVIRLSIPQL 147

Query: 260 TSERRKVFSFAFANIFEQ 278
           T++RRK  S   A   E+
Sbjct: 148 TADRRKELSKIVAKQAEE 165

BLAST of Spg038190 vs. ExPASy Swiss-Prot
Match: P82231 (Ribosome-recycling factor, chloroplastic OS=Spinacia oleracea OX=3562 GN=RRF PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 6.1e-48
Identity = 96/138 (69.57%), Postives = 118/138 (85.51%), Query Frame = 0

Query: 140 RRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNSIRTGRANPAMLDKIEVEYY 199
           R G  R AT+EE+EAEK+LIE + + RMEKTI+T+RS+FNS+RT RA+P MLD+IEVEYY
Sbjct: 72  RAGTFRCATMEEVEAEKSLIETNTKQRMEKTIETIRSNFNSVRTNRASPTMLDRIEVEYY 131

Query: 200 GSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSDLGLTPNNDGEVIRLTLPQL 259
           G+PVSLKSIAQISTPDASSLL+ PYDKSSLK+IEKAIV S LG++PNNDGEVIRL+LP L
Sbjct: 132 GTPVSLKSIAQISTPDASSLLISPYDKSSLKAIEKAIVTSQLGVSPNNDGEVIRLSLPPL 191

Query: 260 TSERRKVFSFAFANIFEQ 278
           TS+RRK  +   + + E+
Sbjct: 192 TSDRRKELAKVVSKLAEE 209

BLAST of Spg038190 vs. ExPASy Swiss-Prot
Match: A2YMU2 (Ribosome-recycling factor, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_26546 PE=1 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 8.7e-47
Identity = 94/138 (68.12%), Postives = 120/138 (86.96%), Query Frame = 0

Query: 140 RRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNSIRTGRANPAMLDKIEVEYY 199
           +R V+R ATIEEIEAEK++IE   R RMEK I+TV+++FN++RTGRANPAMLD+IEVEYY
Sbjct: 67  KRAVLRHATIEEIEAEKSVIEDQARERMEKAIETVQNNFNTVRTGRANPAMLDRIEVEYY 126

Query: 200 GSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSDLGLTPNNDGEVIRLTLPQL 259
           G+PV+LKSIAQI+TPDA+SLL+QPYDKSSLK IEK IV ++LG+TP+NDGEVIR+T+P L
Sbjct: 127 GTPVNLKSIAQINTPDATSLLIQPYDKSSLKLIEKTIVAANLGVTPSNDGEVIRVTVPPL 186

Query: 260 TSERRKVFSFAFANIFEQ 278
           TS+RRK  +   A + E+
Sbjct: 187 TSDRRKELAKTVAKLAEE 204

BLAST of Spg038190 vs. ExPASy Swiss-Prot
Match: A3BLC3 (Ribosome-recycling factor, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0570700 PE=2 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 8.7e-47
Identity = 94/138 (68.12%), Postives = 120/138 (86.96%), Query Frame = 0

Query: 140 RRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNSIRTGRANPAMLDKIEVEYY 199
           +R V+R ATIEEIEAEK++IE   R RMEK I+TV+++FN++RTGRANPAMLD+IEVEYY
Sbjct: 67  KRAVLRHATIEEIEAEKSVIEDQARERMEKAIETVQNNFNTVRTGRANPAMLDRIEVEYY 126

Query: 200 GSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSDLGLTPNNDGEVIRLTLPQL 259
           G+PV+LKSIAQI+TPDA+SLL+QPYDKSSLK IEK IV ++LG+TP+NDGEVIR+T+P L
Sbjct: 127 GTPVNLKSIAQINTPDATSLLIQPYDKSSLKLIEKTIVAANLGVTPSNDGEVIRVTVPPL 186

Query: 260 TSERRKVFSFAFANIFEQ 278
           TS+RRK  +   A + E+
Sbjct: 187 TSDRRKELAKTVAKLAEE 204

BLAST of Spg038190 vs. ExPASy TrEMBL
Match: A0A6J1K744 (Ribosome-recycling factor, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111491268 PE=3 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 1.3e-80
Identity = 178/277 (64.26%), Postives = 193/277 (69.68%), Query Frame = 0

Query: 1   MATPFSSTTSVRSVFQPKQKPPPKALLSFRVDYNGWLNYAKCGSSTVHLCSWKSASNFLS 60
           MATPFSSTTS+R VFQPKQ PPPKALLS R DYN  LN  KCGS+T+HL SWKSASN+  
Sbjct: 1   MATPFSSTTSLRPVFQPKQIPPPKALLSIR-DYNAGLNCVKCGSTTMHLSSWKSASNYFR 60

Query: 61  LKRYALVLSTQLFVSGNLLKNRLSYFFHFIYFLTVLVALCHHFAFGFVIALLLREVYCGG 120
           ++R+AL+L TQ F SGNL K                                        
Sbjct: 61  IRRHALMLPTQPFPSGNLSK---------------------------------------- 120

Query: 121 FSVVLTFFSPSFFLAMTIFRRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNS 180
                             +RRGV+RAATIEEIEAEK LIEKDVRARMEKTIDTVRSSFNS
Sbjct: 121 ------------------YRRGVIRAATIEEIEAEKALIEKDVRARMEKTIDTVRSSFNS 180

Query: 181 IRTGRANPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSD 240
           IRTGRANPAMLDKIEVEYYGSPV LKSIAQISTPDASSLL+QPYDKSSLK IEKAIVNS+
Sbjct: 181 IRTGRANPAMLDKIEVEYYGSPVGLKSIAQISTPDASSLLIQPYDKSSLKLIEKAIVNSN 218

Query: 241 LGLTPNNDGEVIRLTLPQLTSERRKVFSFAFANIFEQ 278
           LG+TPNNDG+VIRLTLPQLTSERRK  S   A   E+
Sbjct: 241 LGMTPNNDGDVIRLTLPQLTSERRKELSKVVAKQAEE 218

BLAST of Spg038190 vs. ExPASy TrEMBL
Match: A0A6J1ESI6 (Ribosome-recycling factor, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111437377 PE=3 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 1.3e-80
Identity = 179/277 (64.62%), Postives = 194/277 (70.04%), Query Frame = 0

Query: 1   MATPFSSTTSVRSVFQPKQKPPPKALLSFRVDYNGWLNYAKCGSSTVHLCSWKSASNFLS 60
           MATPFSSTTS+RSVF+PKQ PPPKALLS R DYN  LN  KCGS+T  L SWKSASN++ 
Sbjct: 1   MATPFSSTTSLRSVFRPKQIPPPKALLSIR-DYNAGLNCIKCGSTTTPLSSWKSASNYIR 60

Query: 61  LKRYALVLSTQLFVSGNLLKNRLSYFFHFIYFLTVLVALCHHFAFGFVIALLLREVYCGG 120
           ++R+AL+L TQ F SGNL K                                        
Sbjct: 61  IRRHALMLPTQPFQSGNLSK---------------------------------------- 120

Query: 121 FSVVLTFFSPSFFLAMTIFRRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNS 180
                             +RRGV+RAATIEEIEAEK LIEKDVRARMEKTIDTVRSSFNS
Sbjct: 121 ------------------YRRGVIRAATIEEIEAEKALIEKDVRARMEKTIDTVRSSFNS 180

Query: 181 IRTGRANPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSD 240
           IRTGRANPAMLDKIEVEYYGSPV LKSIAQISTPDASSLL+QPYDKSSLKSIEKAIVNS+
Sbjct: 181 IRTGRANPAMLDKIEVEYYGSPVGLKSIAQISTPDASSLLIQPYDKSSLKSIEKAIVNSN 218

Query: 241 LGLTPNNDGEVIRLTLPQLTSERRKVFSFAFANIFEQ 278
           LG+TPNNDGEVIRLTLPQLTSERRK  S   A   E+
Sbjct: 241 LGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEE 218

BLAST of Spg038190 vs. ExPASy TrEMBL
Match: A0A6J1ESQ7 (Ribosome-recycling factor, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111437377 PE=3 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 1.3e-80
Identity = 179/277 (64.62%), Postives = 194/277 (70.04%), Query Frame = 0

Query: 1   MATPFSSTTSVRSVFQPKQKPPPKALLSFRVDYNGWLNYAKCGSSTVHLCSWKSASNFLS 60
           MATPFSSTTS+RSVF+PKQ PPPKALLS R DYN  LN  KCGS+T  L SWKSASN++ 
Sbjct: 1   MATPFSSTTSLRSVFRPKQIPPPKALLSIR-DYNAGLNCIKCGSTTTPLSSWKSASNYIR 60

Query: 61  LKRYALVLSTQLFVSGNLLKNRLSYFFHFIYFLTVLVALCHHFAFGFVIALLLREVYCGG 120
           ++R+AL+L TQ F SGNL K                                        
Sbjct: 61  IRRHALMLPTQPFQSGNLSK---------------------------------------- 120

Query: 121 FSVVLTFFSPSFFLAMTIFRRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNS 180
                             +RRGV+RAATIEEIEAEK LIEKDVRARMEKTIDTVRSSFNS
Sbjct: 121 ------------------YRRGVIRAATIEEIEAEKALIEKDVRARMEKTIDTVRSSFNS 180

Query: 181 IRTGRANPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSD 240
           IRTGRANPAMLDKIEVEYYGSPV LKSIAQISTPDASSLL+QPYDKSSLKSIEKAIVNS+
Sbjct: 181 IRTGRANPAMLDKIEVEYYGSPVGLKSIAQISTPDASSLLIQPYDKSSLKSIEKAIVNSN 218

Query: 241 LGLTPNNDGEVIRLTLPQLTSERRKVFSFAFANIFEQ 278
           LG+TPNNDGEVIRLTLPQLTSERRK  S   A   E+
Sbjct: 241 LGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEE 218

BLAST of Spg038190 vs. ExPASy TrEMBL
Match: A0A6J1ETB9 (Ribosome-recycling factor, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111437377 PE=3 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 1.3e-80
Identity = 179/277 (64.62%), Postives = 194/277 (70.04%), Query Frame = 0

Query: 1   MATPFSSTTSVRSVFQPKQKPPPKALLSFRVDYNGWLNYAKCGSSTVHLCSWKSASNFLS 60
           MATPFSSTTS+RSVF+PKQ PPPKALLS R DYN  LN  KCGS+T  L SWKSASN++ 
Sbjct: 1   MATPFSSTTSLRSVFRPKQIPPPKALLSIR-DYNAGLNCIKCGSTTTPLSSWKSASNYIR 60

Query: 61  LKRYALVLSTQLFVSGNLLKNRLSYFFHFIYFLTVLVALCHHFAFGFVIALLLREVYCGG 120
           ++R+AL+L TQ F SGNL K                                        
Sbjct: 61  IRRHALMLPTQPFQSGNLSK---------------------------------------- 120

Query: 121 FSVVLTFFSPSFFLAMTIFRRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNS 180
                             +RRGV+RAATIEEIEAEK LIEKDVRARMEKTIDTVRSSFNS
Sbjct: 121 ------------------YRRGVIRAATIEEIEAEKALIEKDVRARMEKTIDTVRSSFNS 180

Query: 181 IRTGRANPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSD 240
           IRTGRANPAMLDKIEVEYYGSPV LKSIAQISTPDASSLL+QPYDKSSLKSIEKAIVNS+
Sbjct: 181 IRTGRANPAMLDKIEVEYYGSPVGLKSIAQISTPDASSLLIQPYDKSSLKSIEKAIVNSN 218

Query: 241 LGLTPNNDGEVIRLTLPQLTSERRKVFSFAFANIFEQ 278
           LG+TPNNDGEVIRLTLPQLTSERRK  S   A   E+
Sbjct: 241 LGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEE 218

BLAST of Spg038190 vs. ExPASy TrEMBL
Match: A0A1S3BMN4 (Ribosome-recycling factor, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491322 PE=3 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 6.2e-80
Identity = 182/278 (65.47%), Postives = 195/278 (70.14%), Query Frame = 0

Query: 1   MATPFSSTTSV-RSVFQPKQKPPPKALLSFRVDYNGWLNYAKCGSSTVHLCSWKSASNFL 60
           MATPFS+TTS+ RS+F     PPPK LLS R DYNG LN  KCGSS+ HLCSWKSASN++
Sbjct: 1   MATPFSATTSIRRSLF---HNPPPKPLLSLR-DYNGGLNCVKCGSSSAHLCSWKSASNYV 60

Query: 61  SLKRYALVLSTQLFVSGNLLKNRLSYFFHFIYFLTVLVALCHHFAFGFVIALLLREVYCG 120
            L + AL+LSTQL VSGN+LKN                                      
Sbjct: 61  RLNQRALLLSTQLSVSGNVLKN-------------------------------------- 120

Query: 121 GFSVVLTFFSPSFFLAMTIFRRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFN 180
                               RRGVVRAATIEEIEAEKTLIEKDV+ARMEKTIDTVRS+FN
Sbjct: 121 --------------------RRGVVRAATIEEIEAEKTLIEKDVKARMEKTIDTVRSNFN 180

Query: 181 SIRTGRANPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNS 240
           SIRTGR+NPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNS
Sbjct: 181 SIRTGRSNPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNS 216

Query: 241 DLGLTPNNDGEVIRLTLPQLTSERRKVFSFAFANIFEQ 278
           DLGLTPNNDGEVIRLTLPQLTSERRK  S   A   E+
Sbjct: 241 DLGLTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEE 216

BLAST of Spg038190 vs. TAIR 10
Match: AT3G63190.1 (ribosome recycling factor, chloroplast precursor )

HSP 1 Score: 204.5 bits (519), Expect = 1.1e-52
Identity = 106/138 (76.81%), Postives = 124/138 (89.86%), Query Frame = 0

Query: 140 RRGVVRAATIEEIEAEKTLIEKDVRARMEKTIDTVRSSFNSIRTGRANPAMLDKIEVEYY 199
           +R V+R+ATIEEIEAEK+ IE DV+++MEKTI+T+R+SFNSIRTGR+N AMLDKIEVEYY
Sbjct: 76  KRLVIRSATIEEIEAEKSAIETDVKSKMEKTIETLRTSFNSIRTGRSNAAMLDKIEVEYY 135

Query: 200 GSPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSDLGLTPNNDGEVIRLTLPQL 259
           GSPVSLKSIAQISTPD SSLL+QPYDKSSLK+IEKAIVNSDLG+TPNNDG+VIRL+LP L
Sbjct: 136 GSPVSLKSIAQISTPDGSSLLLQPYDKSSLKAIEKAIVNSDLGVTPNNDGDVIRLSLPPL 195

Query: 260 TSERRKVFSFAFANIFEQ 278
           TS+RRK  S   A   E+
Sbjct: 196 TSDRRKELSKVVAKQSEE 213

BLAST of Spg038190 vs. TAIR 10
Match: AT3G01800.1 (Ribosome recycling factor )

HSP 1 Score: 80.5 bits (197), Expect = 2.4e-15
Identity = 43/98 (43.88%), Postives = 58/98 (59.18%), Query Frame = 0

Query: 165 ARMEKTIDTVRSSFNSIRTGRANPAMLDKIEVEYYGSPVSLKSIAQISTPDASSLLVQPY 224
           ++ME  ID +      +RTGRA P MLD I VE  G  + L  +A +S  D  +L V PY
Sbjct: 95  SQMEAAIDALSRDLTKLRTGRAAPGMLDHIVVETGGVKMPLNHLALVSVLDPKTLSVNPY 154

Query: 225 DKSSLKSIEKAIVNSDLGLTPNNDGEVIRLTLPQLTSE 263
           D  ++K +EKAIV S LGL P  DG+ +  ++P LT E
Sbjct: 155 DPDTVKELEKAIVASPLGLNPKLDGQRLVASIPALTKE 192

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888223.13.0e-8468.71ribosome-recycling factor, chloroplastic isoform X1 [Benincasa hispida][more]
XP_038888224.11.9e-8368.35ribosome-recycling factor, chloroplastic isoform X2 [Benincasa hispida][more]
XP_023533411.14.7e-8265.34ribosome-recycling factor, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022995869.12.6e-8064.26ribosome-recycling factor, chloroplastic isoform X1 [Cucurbita maxima][more]
XP_022931084.12.6e-8064.62ribosome-recycling factor, chloroplastic isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9M1X01.5e-5176.81Ribosome-recycling factor, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RRF ... [more]
P377065.5e-4972.46Ribosome-recycling factor, chloroplastic (Fragment) OS=Daucus carota OX=4039 GN=... [more]
P822316.1e-4869.57Ribosome-recycling factor, chloroplastic OS=Spinacia oleracea OX=3562 GN=RRF PE=... [more]
A2YMU28.7e-4768.12Ribosome-recycling factor, chloroplastic OS=Oryza sativa subsp. indica OX=39946 ... [more]
A3BLC38.7e-4768.12Ribosome-recycling factor, chloroplastic OS=Oryza sativa subsp. japonica OX=3994... [more]
Match NameE-valueIdentityDescription
A0A6J1K7441.3e-8064.26Ribosome-recycling factor, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
A0A6J1ESI61.3e-8064.62Ribosome-recycling factor, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1ESQ71.3e-8064.62Ribosome-recycling factor, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1ETB91.3e-8064.62Ribosome-recycling factor, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A1S3BMN46.2e-8065.47Ribosome-recycling factor, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491322... [more]
Match NameE-valueIdentityDescription
AT3G63190.11.1e-5276.81ribosome recycling factor, chloroplast precursor [more]
AT3G01800.12.4e-1543.88Ribosome recycling factor [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036191RRF superfamilyGENE3D1.10.132.20coord: 166..270
e-value: 8.0E-42
score: 144.6
IPR036191RRF superfamilySUPERFAMILY55194Ribosome recycling factor, RRFcoord: 159..267
IPR023584Ribosome recycling factor domainPFAMPF01765RRFcoord: 176..275
e-value: 3.8E-35
score: 121.2
NoneNo IPR availableGENE3D3.30.1360.40coord: 185..259
e-value: 8.0E-42
score: 144.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR20982:SF3MITOCHONDRIAL RIBOSOME RECYCLING FACTORcoord: 82..276
IPR002661Ribosome recycling factorPANTHERPTHR20982RIBOSOME RECYCLING FACTORcoord: 82..276
IPR002661Ribosome recycling factorCDDcd00520RRFcoord: 159..265
e-value: 5.29576E-52
score: 166.281

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg038190.1Spg038190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032544 plastid translation
biological_process GO:0006412 translation
cellular_component GO:0009507 chloroplast
cellular_component GO:0005739 mitochondrion
molecular_function GO:0043023 ribosomal large subunit binding