Spg038068 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg038068
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionTranscription initiation factor IIF subunit alpha
Locationscaffold12: 41825216 .. 41829937 (+)
RNA-Seq ExpressionSpg038068
SyntenySpg038068
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCATTCGATTTGCTGCTGAAGCCATCGTGCAGTGGATGCGGATCCACGACGGAGTTGTACGGGAGCAATTGCAAGCACATGACATTGTGCTTGACCTGTGGCAAAACGATGGCGGAGAACAAGGGCAAATGTTATGACTGTGGCGCTACTGTAACTCGCTTAATAAGAGTTCGTCCTCTTCCTCGTTTTTCTTAAGGAGCATGATGATTTGAATGTGTTGAATTTGTACTCATGTGATTAGGACTTCTTATTTATGACAGATTTAAGAATTTTCATAATAGCGTGGATTGAGTACTGTCTGCCTTCTCTTTCTGAGAGTGCATGATGAACTATATTTTAGGAATACAATGTTCGAGCAAGTGCGAGTAGCGACAAGAACTACTTCATTGGTAGGTTCATGAGTGGGCTGCCAAACTTTTCGAAGAAGAGAAATGCTGAGAATAAATGGTCTCTTTACAAGGAAGGTCTACAAGGACGCCAGCTTACTGATGCCCTGCGGGTACTTAGTTGACTTCTATTTTGCTTCCTCTCTTATGTTTGTATTTGGATAGTCTATTGTTTTGGAAGCCTGGATAGTGGTGACATGATATGTATTTTTCCTTTAGAAGTATAATTGTTGAGAGAAAATAGAGCAAAAGAATCGATGCTATCCTAAAGGGAACATACATTCTTTAACACGGATAAATGGTCTCTTATGGACTTGGAGTCTCGTGCACTTTTCTTTTTCCACTTCTTGGGGCTTCTGCCACAGTAGATGACGGAAATATTTAGTCTTTGTATTAGCTAGCACTTTTCTATTTTTATTTTATATGACTTACATCTATGTTGGACCATGTAATCATATGCTTTGTATTTCATTATTTGTATATTACCTTGTGTTAATCTAATCGTACTTCAACTTTGACCTTGGGGATGCATTTGGTTAATTTCTTCTTGTATTTATTGTTTCTTGTTTCTGACTTCAAGTTAGTTGTTTTGTGGCTTAGTGCTCATTTTCTTTCTTTCTTTCTTTTTTTAAATTTTTATTTGCAATCTTGTCTAATTTGACCCTGATAAATTATGATTTCATTAACACAGGAAAAGTACAAGAATAGACCCTGGCTCTTGGAAGATGAAACGGGCCAATTTCAATTCCAAGGTCATCTTGAGGGTTCACAATCAGCAACGTATTACTTGCTTGTATTGCAAGGAAAGGAATTTACTGCCATTCCGGCAGGTTCTTGGTACGGTTGATCTTTGAGTTAGTTTTCTATGAATCAAGTTATAGCCTTTTATTTGTTTTGCAGTCATCTTCATAAATGGTTATTACATTTTCCAATTCTTGAAGGTTTCTATATTTTCTACTTGTCTTGGATGGAAAATTTCAAACCATCTATGGTGATAAAGGGTTGACTGACCTCAATTTACAGGTACAACTTCAACAAAGTGGCACAATATAAACAACTTACATTGGAGGAGGCAGAAGAGAAAATGAAGAATCGAAAGAAAACAGCAGATGGATATGAAAGATGGATGATGAAAGCTGCAAGTAATGGTCCTGCTGCATTTGGAGAAGTTGAGAAGTTTGATGACAAAGATAGCGTTGGTGCTAGTGGTAGGGGAGGGGGACGCAGAAAGCCTACTGGTGATGAGAATGAGGGTAATGCCTCAGATAGGGGAGAAGAAGATGAAGAAGAAGAGGCAGCAAGGAAGAACAGATTGGGACTCAATAAAAAGGGTGGTGGCGATGATGATGATGAGGAAGGGGCTAGGGGAGGTGACCATGACTTGGATGATGATGATATTGAGAAAGGTTATCTTTTCCTTTTTAAGCAAAACGAATAATTTTTTTTTAGGTCAAGTGCTTTACCATGTCATTGTCATCTCTAAAAAAACTTCACTTGCCATCTGTTACTGGATAGCCATCATATGGGAATCAAATGCATCAAATGAATGGTTTAGAGTTGGGGATAGCATTAGATGAACAATTTCTTTTGTTCTTATCCAAGAAGATATTTGAATTTTCACCGTGCTATTAATTCACATGTTGAGCATTGAGGCCACTGCTATTGTTCCTCTACGTTAACATATCTGTCCAACTTTCTAGGAGATGATTGGGAGCACGAAGAGATTTTTACTGATGATGATGAAACTACTGCTCCGGATCGTGAAGAAAGGGAGGATTTAGCCCCTGAAGTTCCAGCACCTCCAGAAATCAAGCAGGTTGTCTCATTGATACATATCATTTCATCCGTTTACTAGAAACAATTCCATATAGTTGAACAGTTGAAGTAACTGTTTGAAGTTTTGTGCAAAAGAGAGAGAGAGAGAGAGAGAGAGAAAGGAAATGAAAAAAAAAAGCTAATACAGGATGGGTGATTGAAAATAGGAAAGTTGGAGAGGAGATTATGAATGACCATAATTTTTTGCTGAAAGTAGATCCTATGAGTCTTGATCTTGACCACCAACCACATTATTTACCTCCTGATATTAAGATGTAATTATGCAATTCATGTCAGATGTTTGCAATCAAGAGTATTGTCAGGATTTTAGAATACTTGCTTACAGGATTTACAACCTGAGCCTAAAATATGGAGTTATATTTAATAAGGAAAAGGAAAAAAGGAAGGTGTGCGAGGATGAGAAGTTATTTTGGCATGGAAGTTAGTTATTTACGAAATAGTAGTTATGGACGTTGTGTTATCATTTAATTTTTTATTTTTCGTAGAATATGTGGCTGTCTTCATTGTGTTACTATTTTATATTTTTTTAACATGCCCAAGAACAATTCTTAAGACTATTGGGGTATGTGTTCTCTTCGATTGCCTAGGCTAGCCAATTTGATTTTCAATGTGAAACATCACTGACATTTTTGTGATTTTGTTTTGCCGTAGCAACCTGAAGCCCCTAATATAACTAACACTTTCTTAATGTTTGATTTCCATTGACCTTTAAGATCACCTGGAGTTCTAGTTGACATCTTGTATTGTCTGAAGCTTTTCCTGAATATAGTGTCACCTTTCAAGTCAAATATATTTTACATATCAGATACAGGTTGTGTGTTTGTAAAACTAAGCTTTCTACTTCTGTAAATTATTTACTCACTATGCAGGATGAAGACGATGAGGATGAAGAAAATGAAGGGGAAGGGGGCCTGAGCAAATCTGGGAAAGAGTTGAAGAAATTACTTAGGCGTACTGGAGGGCTTAATGATTCAGATGGAGAGGATGACGACGACGATGATGATGTAAGAAGCTCTTGCGATTCCTGTTTACAAAATTTAACAGCACGATACAAAATTTCAAATAATGACCTATGAGCAGTTGTTTGCATCGAATCTGTGTGACTTGCACTCTCTCATTTCTCATGGTATTTTTATGTGTTTGAAGAAGCATTTGATAATGTATTATGCTGTTTGCAATATCTTTTTCCATGTAGTAAATGATAGGACTTCCATTAGTGACAAAGAAGATCTGTTTGCAGTGTCCTGAAGTTTCTTGGAATCCTAACTGACCTGATTGTATCAGTATTTATATATAACCCTCTGCTTATTGTGATACTGCCATTTGTATGTAATTAAGAGACTTTGTACTTTTTATTTGTTCAGGCAGAAGAGGAGAGTGGTGGCACTCCAGTTGTGCCTTCGAAGCAGAAGGACACAGTTAAAGAAGAAGCTCCCGCCAGCAGTCCCTCAAAACCAACACCTTCTGGATCTACGAAGGGAACCCCTTCTACTGCAAAGTCAGCAAAGGGGAAAAGAAAAAATGGCGAGGATGTGAAACCATCCAGTGCACCTTCAAAGAAAATGAAAACAGAGACGGTATGTCATGTCATCTCATGGTTTGAGCAAGTACCTTTATTTGTATTAGAAGGATAAGTAGCTTAAGTCGTTCTTCATCAAACAAAAATTTCTTCTGATATTCAGGAATCAAAGTCTACAGATGAAGGCGTGTCTACTTCTAAAAGTACAGCCTCGAAAGCTGCAGCAGCATCAGTCAAAACAGAACCAGCATCGGGTTCTGGGCCCGTTACTGAGGAAGAAATTAGGGCTGTTTTGCGGCAAAGGACACCTGTTACTACACAGGATCTTGTTGCAAAATTCAAAGCAAGACTACGATCACAAGAGGTATGTTATGTCTTCATGGCATGTTTTGAATAAACTGTTCTTTCTTTCAATGCATCAATATGTTGATGAAACCAAGTTACAGTTTTAGGTTGAGATGTGGCGACTTTATGGTTTAGGGTGTTATATTCAGAAACTTGATGGAGTGTGGCAATTCTCTGATGCCTTATTGTTATTCCATTATGCAGGACAAGAATGCTTTTGCAGAAATACTGAGGAGAATTTCGAGGATTCAAAAGACGAATGGAGGACCGAGTTATGTCGTGTTGAGAAAACCTGAAGACTAGAAGAATTAAATTTAAGTTTCTTCTTTGTGATGTAGCTGGTTCATGGTTTCTAACTGTTTGGTGGGTTATGGTCATCCAGTACGCAATGCTTGTGCTGCAAAAGCTTGAGGTCCTCCTCTTTCACATGTGAAGCACTTTTATGTGGCACTTTATCTATGAAAAGGATATAAATTTGTGACTTTCAAAGAGCGTTGGAATTTAAGTTGTAAGAGATTGGTATATATACATTGAGAAAGAAACGAAAAATTTTACAGACCCTTTGTCATATTGGTTGTCATATTGATCCTGTCATTTTCTTAAAACGTTTCTAAAATAATGCAATGAATAAGC

mRNA sequence

ATGTCATTCGATTTGCTGCTGAAGCCATCGTGCAGTGGATGCGGATCCACGACGGAGTTGTACGGGAGCAATTGCAAGCACATGACATTGTGCTTGACCTGTGGCAAAACGATGGCGGAGAACAAGGGCAAATGTTATGACTGTGGCGCTACTGAATACAATGTTCGAGCAAGTGCGAGTAGCGACAAGAACTACTTCATTGGTAGGTTCATGAGTGGGCTGCCAAACTTTTCGAAGAAGAGAAATGCTGAGAATAAATGGTCTCTTTACAAGGAAGGTCTACAAGGACGCCAGCTTACTGATGCCCTGCGGGAAAAGTACAAGAATAGACCCTGGCTCTTGGAAGATGAAACGGGCCAATTTCAATTCCAAGGTCATCTTGAGGGTTCACAATCAGCAACGTATTACTTGCTTGTATTGCAAGGAAAGGAATTTACTGCCATTCCGGCAGGTTCTTGGTACAACTTCAACAAAGTGGCACAATATAAACAACTTACATTGGAGGAGGCAGAAGAGAAAATGAAGAATCGAAAGAAAACAGCAGATGGATATGAAAGATGGATGATGAAAGCTGCAAGTAATGGTCCTGCTGCATTTGGAGAAGTTGAGAAGTTTGATGACAAAGATAGCGTTGGTGCTAGTGGTAGGGGAGGGGGACGCAGAAAGCCTACTGGTGATGAGAATGAGGGTAATGCCTCAGATAGGGGAGAAGAAGATGAAGAAGAAGAGGCAGCAAGGAAGAACAGATTGGGACTCAATAAAAAGGGTGGTGGCGATGATGATGATGAGGAAGGGGCTAGGGGAGGTGACCATGACTTGGATGATGATGATATTGAGAAAGGAGATGATTGGGAGCACGAAGAGATTTTTACTGATGATGATGAAACTACTGCTCCGGATCGTGAAGAAAGGGAGGATTTAGCCCCTGAAGTTCCAGCACCTCCAGAAATCAAGCAGGATGAAGACGATGAGGATGAAGAAAATGAAGGGGAAGGGGGCCTGAGCAAATCTGGGAAAGAGTTGAAGAAATTACTTAGGCGTACTGGAGGGCTTAATGATTCAGATGGAGAGGATGACGACGACGATGATGATGCAGAAGAGGAGAGTGGTGGCACTCCAGTTGTGCCTTCGAAGCAGAAGGACACAGTTAAAGAAGAAGCTCCCGCCAGCAGTCCCTCAAAACCAACACCTTCTGGATCTACGAAGGGAACCCCTTCTACTGCAAAGTCAGCAAAGGGGAAAAGAAAAAATGGCGAGGATGTGAAACCATCCAGTGCACCTTCAAAGAAAATGAAAACAGAGACGGAATCAAAGTCTACAGATGAAGGCGTGTCTACTTCTAAAAGTACAGCCTCGAAAGCTGCAGCAGCATCAGTCAAAACAGAACCAGCATCGGGTTCTGGGCCCGTTACTGAGGAAGAAATTAGGGCTGTTTTGCGGCAAAGGACACCTGTTACTACACAGGATCTTGTTGCAAAATTCAAAGCAAGACTACGATCACAAGAGGACAAGAATGCTTTTGCAGAAATACTGAGGAGAATTTCGAGGATTCAAAAGACGAATGGAGGACCGAGTTATGTCGTGTTGAGAAAACCTGAAGACTAG

Coding sequence (CDS)

ATGTCATTCGATTTGCTGCTGAAGCCATCGTGCAGTGGATGCGGATCCACGACGGAGTTGTACGGGAGCAATTGCAAGCACATGACATTGTGCTTGACCTGTGGCAAAACGATGGCGGAGAACAAGGGCAAATGTTATGACTGTGGCGCTACTGAATACAATGTTCGAGCAAGTGCGAGTAGCGACAAGAACTACTTCATTGGTAGGTTCATGAGTGGGCTGCCAAACTTTTCGAAGAAGAGAAATGCTGAGAATAAATGGTCTCTTTACAAGGAAGGTCTACAAGGACGCCAGCTTACTGATGCCCTGCGGGAAAAGTACAAGAATAGACCCTGGCTCTTGGAAGATGAAACGGGCCAATTTCAATTCCAAGGTCATCTTGAGGGTTCACAATCAGCAACGTATTACTTGCTTGTATTGCAAGGAAAGGAATTTACTGCCATTCCGGCAGGTTCTTGGTACAACTTCAACAAAGTGGCACAATATAAACAACTTACATTGGAGGAGGCAGAAGAGAAAATGAAGAATCGAAAGAAAACAGCAGATGGATATGAAAGATGGATGATGAAAGCTGCAAGTAATGGTCCTGCTGCATTTGGAGAAGTTGAGAAGTTTGATGACAAAGATAGCGTTGGTGCTAGTGGTAGGGGAGGGGGACGCAGAAAGCCTACTGGTGATGAGAATGAGGGTAATGCCTCAGATAGGGGAGAAGAAGATGAAGAAGAAGAGGCAGCAAGGAAGAACAGATTGGGACTCAATAAAAAGGGTGGTGGCGATGATGATGATGAGGAAGGGGCTAGGGGAGGTGACCATGACTTGGATGATGATGATATTGAGAAAGGAGATGATTGGGAGCACGAAGAGATTTTTACTGATGATGATGAAACTACTGCTCCGGATCGTGAAGAAAGGGAGGATTTAGCCCCTGAAGTTCCAGCACCTCCAGAAATCAAGCAGGATGAAGACGATGAGGATGAAGAAAATGAAGGGGAAGGGGGCCTGAGCAAATCTGGGAAAGAGTTGAAGAAATTACTTAGGCGTACTGGAGGGCTTAATGATTCAGATGGAGAGGATGACGACGACGATGATGATGCAGAAGAGGAGAGTGGTGGCACTCCAGTTGTGCCTTCGAAGCAGAAGGACACAGTTAAAGAAGAAGCTCCCGCCAGCAGTCCCTCAAAACCAACACCTTCTGGATCTACGAAGGGAACCCCTTCTACTGCAAAGTCAGCAAAGGGGAAAAGAAAAAATGGCGAGGATGTGAAACCATCCAGTGCACCTTCAAAGAAAATGAAAACAGAGACGGAATCAAAGTCTACAGATGAAGGCGTGTCTACTTCTAAAAGTACAGCCTCGAAAGCTGCAGCAGCATCAGTCAAAACAGAACCAGCATCGGGTTCTGGGCCCGTTACTGAGGAAGAAATTAGGGCTGTTTTGCGGCAAAGGACACCTGTTACTACACAGGATCTTGTTGCAAAATTCAAAGCAAGACTACGATCACAAGAGGACAAGAATGCTTTTGCAGAAATACTGAGGAGAATTTCGAGGATTCAAAAGACGAATGGAGGACCGAGTTATGTCGTGTTGAGAAAACCTGAAGACTAG

Protein sequence

MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATEYNVRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLLEDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGGGRRKPTGDENEGNASDRGEEDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTDDDETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGEDDDDDDDAEEESGGTPVVPSKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSAKGKRKNGEDVKPSSAPSKKMKTETESKSTDEGVSTSKSTASKAAAASVKTEPASGSGPVTEEEIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Homology
BLAST of Spg038068 vs. NCBI nr
Match: XP_038888042.1 (transcription initiation factor IIF subunit alpha isoform X1 [Benincasa hispida])

HSP 1 Score: 940.6 bits (2430), Expect = 5.7e-270
Identity = 507/540 (93.89%), Postives = 517/540 (95.74%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT------EYN 60
           MSFDL+LKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT      EYN
Sbjct: 1   MSFDLMLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYK+GLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKDGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGGGRRKPTGDENEGNASD 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK+ VG SGR  GRRKPTGDENEGN SD
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKEGVGTSGR--GRRKPTGDENEGNVSD 240

Query: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTDDD 300
           RGEEDEEEEA RKNRLGLNKKGGGDDDDEEG RGGDHDLDDDDIEKGDDWEHEEIFTDDD
Sbjct: 241 RGEEDEEEEAQRKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDD 300

Query: 301 ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360
           ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS
Sbjct: 301 ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360

Query: 361 DGEDDDDDDDAEEESGGTPVVPSKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSAKGK 420
           DGEDDDDDDD +E++GGTPV+PSKQKDTVKEEA A+SPSKPTPSGS KGTPSTAK+AKGK
Sbjct: 361 DGEDDDDDDDVDEDTGGTPVLPSKQKDTVKEEAAANSPSKPTPSGSAKGTPSTAKTAKGK 420

Query: 421 RKNGEDVKPSSAPSKKMKTETESKSTDEGVSTSKSTASKAAAASVKTEPASGSGPVTEEE 480
           RKNGEDVKPSSAP KKMKTETESKSTDE VSTSKSTASK A A VKTEPAS SGPVTEEE
Sbjct: 421 RKNGEDVKPSSAPPKKMKTETESKSTDE-VSTSKSTASKGAPALVKTEPASHSGPVTEEE 480

Query: 481 IRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 535
           IRAVLRQRTPVTTQDLVAKFKARLR+ EDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 481 IRAVLRQRTPVTTQDLVAKFKARLRTPEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 537

BLAST of Spg038068 vs. NCBI nr
Match: XP_022931161.1 (transcription initiation factor IIF subunit alpha-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 940.3 bits (2429), Expect = 7.5e-270
Identity = 509/541 (94.09%), Postives = 517/541 (95.56%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT------EYN 60
           MS DLLLKPSCSGCGSTTELYGSNCKHMTLCL CGKTMAENKGKCYDCGAT      EYN
Sbjct: 1   MSLDLLLKPSCSGCGSTTELYGSNCKHMTLCLRCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRAS+SSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASSSSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQ QFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQSQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGGGRRKPTGDENEGNASD 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGA+GR  GRRKPT DENEGN SD
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGR--GRRKPTSDENEGNVSD 240

Query: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTDDD 300
           RGEEDEEEEAARKNRLGLNKKGGGDDDDEEG RGG+HDLDDDDIEKGDDWEHEEIFTDDD
Sbjct: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGNHDLDDDDIEKGDDWEHEEIFTDDD 300

Query: 301 ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360
           ETTAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRRTGGLNDS
Sbjct: 301 ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360

Query: 361 DG-EDDDDDDDAEEESGGTPVVPSKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSAKG 420
           DG E+DDDDDDAEEESGGTPV PSKQKD VKEEA A+SPSKP PSGS KGTPSTAK+AKG
Sbjct: 361 DGEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKG 420

Query: 421 KRKNGEDVKPSSAPSKKMKTETESKSTDEGVSTSKSTASKAAAASVKTEPASGSGPVTEE 480
           KRKNGEDVKPSSAP KKMKTETESKSTDEGVSTS+STASK AAASVKTEPAS SGPVTEE
Sbjct: 421 KRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEE 480

Query: 481 EIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 535
           EIRAVLRQRTPVTTQDLVAKFKARLRS EDKNAFAEILRRISRIQKTNGGPSYVVLRKPE
Sbjct: 481 EIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 539

BLAST of Spg038068 vs. NCBI nr
Match: KAG6606328.1 (Transcription initiation factor IIF subunit alpha, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 938.7 bits (2425), Expect = 2.2e-269
Identity = 508/541 (93.90%), Postives = 517/541 (95.56%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT------EYN 60
           MS DLLLKPSCSGCGSTTELYGSNCKHMTLCL CGKTMAENKGKCYDCGAT      EYN
Sbjct: 27  MSLDLLLKPSCSGCGSTTELYGSNCKHMTLCLRCGKTMAENKGKCYDCGATVTRLIREYN 86

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRAS+SSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL
Sbjct: 87  VRASSSSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 146

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQ QFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 147 EDETGQSQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 206

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGGGRRKPTGDENEGNASD 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGA+GR  GRRKPT DENEGN SD
Sbjct: 207 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGR--GRRKPTSDENEGNVSD 266

Query: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTDDD 300
           RGEEDEEEEAARKNRLGLNKKGGGDDDDEEG RGGDHDLDDDDI+KGDDWEHEEIFTDDD
Sbjct: 267 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGHRGGDHDLDDDDIKKGDDWEHEEIFTDDD 326

Query: 301 ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360
           ETTAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRRTGGLNDS
Sbjct: 327 ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 386

Query: 361 DGEDD-DDDDDAEEESGGTPVVPSKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSAKG 420
           DGE++ DDDDDAEEESGGTPV PSKQKD VKEEA A+SPSKP PSGS KGTPSTAK+AKG
Sbjct: 387 DGEEENDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKG 446

Query: 421 KRKNGEDVKPSSAPSKKMKTETESKSTDEGVSTSKSTASKAAAASVKTEPASGSGPVTEE 480
           KRKNGEDVKPSSAP KKMKTETESKSTDEGVSTS+STASK AAASVKTEPAS SGPVTEE
Sbjct: 447 KRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEE 506

Query: 481 EIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 535
           EIRAVLRQRTPVTTQDLVAKFKARLRS EDKNAFAEILRRISRIQKTNGGPSYVVLRKPE
Sbjct: 507 EIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 565

BLAST of Spg038068 vs. NCBI nr
Match: XP_004152016.1 (transcription initiation factor IIF subunit alpha isoform X1 [Cucumis sativus] >XP_011651639.1 transcription initiation factor IIF subunit alpha isoform X1 [Cucumis sativus] >KGN58337.1 hypothetical protein Csa_017587 [Cucumis sativus])

HSP 1 Score: 937.6 bits (2422), Expect = 4.8e-269
Identity = 503/540 (93.15%), Postives = 518/540 (95.93%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT------EYN 60
           MSFDLLLK SCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT      EYN
Sbjct: 1   MSFDLLLKQSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRASAS+DKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQL+DALREKYKNRPWLL
Sbjct: 61  VRASASNDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGGGRRKPTGDENEGNASD 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK++VG SGR  GRRK TGDENEGN SD
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGR--GRRKTTGDENEGNVSD 240

Query: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTDDD 300
           RGEEDEEEEAARKNRLGL+KKGGGDDDDEEG RGGDHDLDDDDIEKGDDWEHEEIFTDDD
Sbjct: 241 RGEEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDD 300

Query: 301 ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360
           ETTAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRRTGGLNDS
Sbjct: 301 ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360

Query: 361 DGEDDDDDDDAEEESGGTPVVPSKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSAKGK 420
           DGEDDDDDDD +E++GGTPV+PSKQKDTVKEEA A+SPSKPTPSGS KGTPSTAK+AKGK
Sbjct: 361 DGEDDDDDDDVDEDTGGTPVLPSKQKDTVKEEAAANSPSKPTPSGSAKGTPSTAKTAKGK 420

Query: 421 RKNGEDVKPSSAPSKKMKTETESKSTDEGVSTSKSTASKAAAASVKTEPASGSGPVTEEE 480
           RKNGEDVKPSSAP KKMKTETESK+TDEGV TSKSTASK A ASVKTEP+S SGPVTEEE
Sbjct: 421 RKNGEDVKPSSAPPKKMKTETESKATDEGVPTSKSTASKGAPASVKTEPSSHSGPVTEEE 480

Query: 481 IRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 535
           IRAVLRQRTPVTTQDLVAKFKARLR+ EDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 481 IRAVLRQRTPVTTQDLVAKFKARLRTPEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538

BLAST of Spg038068 vs. NCBI nr
Match: XP_023532767.1 (transcription initiation factor IIF subunit alpha-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 934.1 bits (2413), Expect = 5.4e-268
Identity = 506/542 (93.36%), Postives = 515/542 (95.02%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT------EYN 60
           M  DLLLKPSCSGCGSTTELYGSNCKHMTLCL+CGKTMAENKGKCYDCGAT      EYN
Sbjct: 1   MPLDLLLKPSCSGCGSTTELYGSNCKHMTLCLSCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRAS+SSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASSSSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQ QFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQSQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGGGRRKPTGDENEGNASD 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGA+GR  GRRKPT D+NEGN SD
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGR--GRRKPTSDDNEGNVSD 240

Query: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTDDD 300
           RGE DEEEEAARKNRLGLNKKGGGDDDDEEG RGGDHDLDDDDIEKGDDWEHEEIFTDDD
Sbjct: 241 RGEGDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDD 300

Query: 301 ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360
           ETTAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLL+RTGGLNDS
Sbjct: 301 ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLKRTGGLNDS 360

Query: 361 DGE--DDDDDDDAEEESGGTPVVPSKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSAK 420
           DGE  DDDDDDDAEEESGGTPV PSKQKD VKEEA A+SPSKP PSGS KGT STAK+AK
Sbjct: 361 DGEDDDDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTSSTAKTAK 420

Query: 421 GKRKNGEDVKPSSAPSKKMKTETESKSTDEGVSTSKSTASKAAAASVKTEPASGSGPVTE 480
           GKRKNGEDVKPSSAP KKMKTETESKSTDEGVSTS+STASK AAASVKTEPAS SGPVTE
Sbjct: 421 GKRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTE 480

Query: 481 EEIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKP 535
           EEIRAVLRQRTPVTTQDLVAKFKARLRS EDKNAFAEILRRISRIQKTNGGPSYVVLRKP
Sbjct: 481 EEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKP 540

BLAST of Spg038068 vs. ExPASy Swiss-Prot
Match: Q9SU25 (Transcription initiation factor IIF subunit alpha OS=Arabidopsis thaliana OX=3702 GN=RAP74 PE=1 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 7.7e-177
Identity = 369/550 (67.09%), Postives = 438/550 (79.64%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT------EYN 60
           MS  L L  SC GCGS ++LYGS+C+HMTLCL CG+TMA+NK KC++CG        EYN
Sbjct: 1   MSNCLQLNTSCVGCGSQSDLYGSSCRHMTLCLKCGRTMAQNKSKCHECGTVVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRA+A +DKNYFIGRF++GLPNF  K+ +ENKWSL K+  QGRQ TDA REK KN+PW+L
Sbjct: 61  VRAAAPTDKNYFIGRFVTGLPNF--KKGSENKWSLRKDIPQGRQFTDAQREKLKNKPWIL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQ+QGHLEGSQSATYYLLV+Q KEF AIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQYQGHLEGSQSATYYLLVMQNKEFVAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGG-GRRKPT-GDENEGNA 240
           KNR+KTADGY+RWMMKAA+NGPA FGEV+  ++K+S G SG GG GR+K + GDE EGN 
Sbjct: 181 KNRRKTADGYQRWMMKAANNGPALFGEVD--NEKESGGTSGGGGRGRKKSSGGDEEEGNV 240

Query: 241 SDRGEEDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTD 300
           SDRG+EDEEEEA+RK+RLGLN+K   DDDDEEG RGGD D+DDDDIEKGDDWEHEEIFTD
Sbjct: 241 SDRGDEDEEEEASRKSRLGLNRK-SNDDDDEEGPRGGDLDMDDDDIEKGDDWEHEEIFTD 300

Query: 301 DDETTAPDREERED-LAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGL 360
           DDE    D EERED LAPE+PAPPEIKQDEDDE+ E E EGGLSKSGKELKKLL +  GL
Sbjct: 301 DDEAVGNDPEEREDLLAPEIPAPPEIKQDEDDEENEEE-EGGLSKSGKELKKLLGKANGL 360

Query: 361 NDSDGEDDDDDDDAEEESGGTPVVPSKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSA 420
           ++SD +DDDD DD EE + GT V  SKQK+  KEE   ++P+KP PSG  +GTP  AK +
Sbjct: 361 DESDEDDDDDSDDEEETNYGT-VTNSKQKEAAKEEPVDNAPAKPAPSGPPRGTP-PAKPS 420

Query: 421 KGKRK--NGEDVKPSSAPSKKMKTETESKST---DEGVSTSKS-TASKAAAASVKTEPAS 480
           KGKRK  +G+  KPSS+  KK+KTE + KS+   +   + SKS T +KA  A   + PAS
Sbjct: 421 KGKRKLNDGDSKKPSSSVQKKVKTENDPKSSLKEERANTVSKSNTPTKAVKAEPASAPAS 480

Query: 481 GS----GPVTEEEIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTN 532
            S    GPVTE+EIRAVL ++  VTTQDLV++FKARL+++EDKNAFA ILR+IS+IQK  
Sbjct: 481 SSSAATGPVTEDEIRAVLMEKKQVTTQDLVSRFKARLKTKEDKNAFANILRKISKIQKNA 540

BLAST of Spg038068 vs. ExPASy Swiss-Prot
Match: Q54WV4 (General transcription factor IIF subunit 1 OS=Dictyostelium discoideum OX=44689 GN=gtf2f1 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 5.6e-10
Identity = 117/432 (27.08%), Postives = 189/432 (43.75%), Query Frame = 0

Query: 108 KNRPWLLEDETGQFQFQGHLEGSQ-SATYYLLVLQGK-EFTAIPAGSWYNFNKVAQYKQL 167
           K  PW LED  G   +QG++EG+Q S+ Y+L + Q      A+P   WYNF    +++ L
Sbjct: 146 KTIPWKLEDSEGNNTYQGNVEGNQASSNYFLFMFQSDGSIKAVPCNDWYNFRPKKEFQSL 205

Query: 168 TLEEAEEKMKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGGGRRKPTG 227
           T EEAE+ MK + +  D +                 ++K  D    G+ G     +K   
Sbjct: 206 TTEEAEDFMKKKNQEWDVFT--------------SRLQKKTDPSGSGSGGSESSGKKSIE 265

Query: 228 DENEGNASDRGE---------EDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDD 287
           +  E    +R E         E+++++ A   R     +G G+D D         D DDD
Sbjct: 266 ELEEAEHRNRNEDPNRYKTTNEEKKKKKAPARRREREDEGNGEDGDAPDFESKFDDDDDD 325

Query: 288 DIEKGDDWEHEEIFTDDDETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSK 347
               GD        T +DE    + EE ED+        E+K+    + ++N+ E  L+ 
Sbjct: 326 TYMDGDG------LTGEDENQIEEDEEEEDV-DLTEGGLEMKKMIKKQQQQNDSEDDLA- 385

Query: 348 SGKELKKLLRRTGGLNDSDGEDDDDDDDAEEESGGTPVVPSKQKD-----TVKEEAPASS 407
              + K     TGG +D D ++DD+D D ++ S    V      D     +VK+E     
Sbjct: 386 DDDDKKDNNGGTGGGDDEDDDEDDEDPDQDDLSNLPKVFGKSNADGTTNSSVKKEDDGGK 445

Query: 408 PSKPTPSGSTKGTPSTAKSAKGK-RKNGEDVKPSSAPSKKMKTETESKSTDEGVSTSKST 467
            SK +     K   S+ KS + K +KN  D    +  SKK+K E  S    +  S S+  
Sbjct: 446 DSKSSKKSKKKDKDSSPKSKEKKSKKNKSDSSVDNRESKKIKKEPSSPQAVQPNSPSQQQ 505

Query: 468 ASKAAAASVKTEPASGSGPVTEEEIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEI 523
             +        +P     P TEE I+ VL++   V + DL+  FK  L++ ++K  F  +
Sbjct: 506 QQQ----QQNIDP--NDPPFTEEYIKIVLQKSKKVKSLDLINIFKGPLKNPDNKPIFLAM 549

BLAST of Spg038068 vs. ExPASy TrEMBL
Match: A0A6J1ESX1 (Transcription initiation factor IIF subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111437424 PE=3 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 3.6e-270
Identity = 509/541 (94.09%), Postives = 517/541 (95.56%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT------EYN 60
           MS DLLLKPSCSGCGSTTELYGSNCKHMTLCL CGKTMAENKGKCYDCGAT      EYN
Sbjct: 1   MSLDLLLKPSCSGCGSTTELYGSNCKHMTLCLRCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRAS+SSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASSSSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQ QFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQSQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGGGRRKPTGDENEGNASD 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGA+GR  GRRKPT DENEGN SD
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGR--GRRKPTSDENEGNVSD 240

Query: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTDDD 300
           RGEEDEEEEAARKNRLGLNKKGGGDDDDEEG RGG+HDLDDDDIEKGDDWEHEEIFTDDD
Sbjct: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGNHDLDDDDIEKGDDWEHEEIFTDDD 300

Query: 301 ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360
           ETTAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRRTGGLNDS
Sbjct: 301 ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360

Query: 361 DG-EDDDDDDDAEEESGGTPVVPSKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSAKG 420
           DG E+DDDDDDAEEESGGTPV PSKQKD VKEEA A+SPSKP PSGS KGTPSTAK+AKG
Sbjct: 361 DGEEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKG 420

Query: 421 KRKNGEDVKPSSAPSKKMKTETESKSTDEGVSTSKSTASKAAAASVKTEPASGSGPVTEE 480
           KRKNGEDVKPSSAP KKMKTETESKSTDEGVSTS+STASK AAASVKTEPAS SGPVTEE
Sbjct: 421 KRKNGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEE 480

Query: 481 EIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 535
           EIRAVLRQRTPVTTQDLVAKFKARLRS EDKNAFAEILRRISRIQKTNGGPSYVVLRKPE
Sbjct: 481 EIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 539

BLAST of Spg038068 vs. ExPASy TrEMBL
Match: A0A0A0LCA6 (Transcription initiation factor IIF subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_3G624050 PE=3 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 2.3e-269
Identity = 503/540 (93.15%), Postives = 518/540 (95.93%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT------EYN 60
           MSFDLLLK SCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT      EYN
Sbjct: 1   MSFDLLLKQSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRASAS+DKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQL+DALREKYKNRPWLL
Sbjct: 61  VRASASNDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGGGRRKPTGDENEGNASD 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK++VG SGR  GRRK TGDENEGN SD
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGR--GRRKTTGDENEGNVSD 240

Query: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTDDD 300
           RGEEDEEEEAARKNRLGL+KKGGGDDDDEEG RGGDHDLDDDDIEKGDDWEHEEIFTDDD
Sbjct: 241 RGEEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDD 300

Query: 301 ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360
           ETTAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRRTGGLNDS
Sbjct: 301 ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360

Query: 361 DGEDDDDDDDAEEESGGTPVVPSKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSAKGK 420
           DGEDDDDDDD +E++GGTPV+PSKQKDTVKEEA A+SPSKPTPSGS KGTPSTAK+AKGK
Sbjct: 361 DGEDDDDDDDVDEDTGGTPVLPSKQKDTVKEEAAANSPSKPTPSGSAKGTPSTAKTAKGK 420

Query: 421 RKNGEDVKPSSAPSKKMKTETESKSTDEGVSTSKSTASKAAAASVKTEPASGSGPVTEEE 480
           RKNGEDVKPSSAP KKMKTETESK+TDEGV TSKSTASK A ASVKTEP+S SGPVTEEE
Sbjct: 421 RKNGEDVKPSSAPPKKMKTETESKATDEGVPTSKSTASKGAPASVKTEPSSHSGPVTEEE 480

Query: 481 IRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 535
           IRAVLRQRTPVTTQDLVAKFKARLR+ EDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 481 IRAVLRQRTPVTTQDLVAKFKARLRTPEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538

BLAST of Spg038068 vs. ExPASy TrEMBL
Match: A0A1S4DWR2 (Transcription initiation factor IIF subunit alpha OS=Cucumis melo OX=3656 GN=LOC103489800 PE=3 SV=1)

HSP 1 Score: 934.1 bits (2413), Expect = 2.6e-268
Identity = 502/540 (92.96%), Postives = 515/540 (95.37%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT------EYN 60
           MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT      EYN
Sbjct: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAE KM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEXKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGGGRRKPTGDENEGNASD 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK++VG SGR  GRRK TGDENEGN SD
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGR--GRRKTTGDENEGNVSD 240

Query: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTDDD 300
           RGEEDEEEEAARKNRLGL+KKGGGDDDDEEG RGGDHDLDDDDIEKGDDWEHEEIFTDDD
Sbjct: 241 RGEEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDD 300

Query: 301 ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360
           ETTAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRRTGGLNDS
Sbjct: 301 ETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360

Query: 361 DGEDDDDDDDAEEESGGTPVVPSKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSAKGK 420
           DGEDD+DDDD +E++GGTPV+PSKQKDTVKEEA A+SPSKPTPSGS KGTPSTAK+AKGK
Sbjct: 361 DGEDDEDDDDVDEDTGGTPVLPSKQKDTVKEEAAANSPSKPTPSGSAKGTPSTAKTAKGK 420

Query: 421 RKNGEDVKPSSAPSKKMKTETESKSTDEGVSTSKSTASKAAAASVKTEPASGSGPVTEEE 480
           RKNGEDVKPSSAP KKMKTETE K TDEGV TSKSTASK A ASVKTEP+S SGPVTEEE
Sbjct: 421 RKNGEDVKPSSAPPKKMKTETELKPTDEGVPTSKSTASKGAPASVKTEPSSHSGPVTEEE 480

Query: 481 IRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 535
           IRAVLRQRTPVTTQDLVAKFKARLR+ EDKNAFAEILRRISRIQK NGGPSYVVLRKPED
Sbjct: 481 IRAVLRQRTPVTTQDLVAKFKARLRTPEDKNAFAEILRRISRIQKINGGPSYVVLRKPED 538

BLAST of Spg038068 vs. ExPASy TrEMBL
Match: A0A6J1K1N5 (Transcription initiation factor IIF subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111491607 PE=3 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.2e-267
Identity = 506/544 (93.01%), Postives = 516/544 (94.85%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT------EYN 60
           MS DLLLKPSCSGCGSTTELYGSNCKHMTLCL+CGKTMAENKGKCYDCGAT      EYN
Sbjct: 1   MSLDLLLKPSCSGCGSTTELYGSNCKHMTLCLSCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRAS+SSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL
Sbjct: 61  VRASSSSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQ QFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQSQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGGGRRKPTGDENEGNASD 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDS+GA+GR  GRRKPT DENEGN SD
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSIGATGR--GRRKPTSDENEGNVSD 240

Query: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDDDIEK-GDDWEHEEIFTDD 300
           RGEEDEEEEAARKNRLGLNKKGGGDDDDEEG RGGDHDLDDDDIE  GDDWEHEEIFTDD
Sbjct: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIESIGDDWEHEEIFTDD 300

Query: 301 DETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLND 360
           DETTAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRR+GGLND
Sbjct: 301 DETTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRSGGLND 360

Query: 361 SDG---EDDDDDDDAEEESGGTPVVPSKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKS 420
           SDG   EDDDDDDDAEEESGGTPV PSKQKD VKEEA A+SPSKP PSGS KGT STAK+
Sbjct: 361 SDGEEEEDDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTSSTAKT 420

Query: 421 AKGKRKNGEDVKPSSAPSKKMKTETESKSTDEGVSTSKSTASKAAAASVKTEPASGSGPV 480
           AKGKRKNGEDVKPSSAP KKMKTETESKSTDEG+STS+STASK AAASVKTEPAS SGPV
Sbjct: 421 AKGKRKNGEDVKPSSAPPKKMKTETESKSTDEGMSTSRSTASKGAAASVKTEPASASGPV 480

Query: 481 TEEEIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLR 535
           TEEEIRAVLRQRTPVTTQDLVAKFKARLRS EDKNAFAEILRRISRIQKTNGGPSYVVLR
Sbjct: 481 TEEEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLR 540

BLAST of Spg038068 vs. ExPASy TrEMBL
Match: A0A6J1E3E1 (Transcription initiation factor IIF subunit alpha OS=Momordica charantia OX=3673 GN=LOC111025673 PE=3 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 3.0e-264
Identity = 500/541 (92.42%), Postives = 515/541 (95.19%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT------EYN 60
           MSFDLLLKPSCSGCGS+T+LYGSNCKHMTLCLTCGKTMAENKGKCYDCGAT      EYN
Sbjct: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120
           VRASASSDKNYFIGRFMSGLPNFS+KRNAENKWSLYKEGLQGRQL+DALREKYKNRPWLL
Sbjct: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGASGRGGGRRKPTGDENEGNASD 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKD+VGASGR  GRRK TGDENEGNASD
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGR--GRRKTTGDENEGNASD 240

Query: 241 RGEEDEEEEAARKNRLGLNKKGGGDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTDDD 300
           RGEEDEEEEAARKNRLGLNKK  GDDDDEEG RGGDHD+DDDDIEKGDDWEHEEIFTDDD
Sbjct: 241 RGEEDEEEEAARKNRLGLNKK-SGDDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDD 300

Query: 301 ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360
           ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS
Sbjct: 301 ETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360

Query: 361 DGEDDDDDDDAEEESGGTPVVPSKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSAKGK 420
           DGEDDDDDDD EE+ GGTPVV SKQKDTVKEEA ASSPSKP PSGS+KGTPS+ K++KGK
Sbjct: 361 DGEDDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGK 420

Query: 421 RK-NGEDVKPSSAPSKKMKTETESKSTDEGVSTSKSTASKAAAASVKTEPASGSGPVTEE 480
           RK NG+DVKPS+ P KK+KTET+SKSTDEGVSTSKSTASK A ASVKTE A  SGPVTEE
Sbjct: 421 RKLNGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEE 480

Query: 481 EIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 535
           EIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE
Sbjct: 481 EIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPE 538

BLAST of Spg038068 vs. TAIR 10
Match: AT4G12610.1 (transcription activators;DNA binding;RNA polymerase II transcription factors;catalytics;transcription initiation factors )

HSP 1 Score: 419.5 bits (1077), Expect = 4.1e-117
Identity = 269/405 (66.42%), Postives = 320/405 (79.01%), Query Frame = 0

Query: 140 LQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRKKTADGYERWMMKAASNGPAAF 199
           +Q KEF AIPAGSWYNFNKVAQYKQLTLEEAEEKMKNR+KTADGY+RWMMKAA+NGPA F
Sbjct: 1   MQNKEFVAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRRKTADGYQRWMMKAANNGPALF 60

Query: 200 GEVEKFDDKDSVGASGRGG-GRRKPT-GDENEGNASDRGEEDEEEEAARKNRLGLNKKGG 259
           GEV+  ++K+S G SG GG GR+K + GDE EGN SDRG+EDEEEEA+RK+RLGLN+K  
Sbjct: 61  GEVD--NEKESGGTSGGGGRGRKKSSGGDEEEGNVSDRGDEDEEEEASRKSRLGLNRK-S 120

Query: 260 GDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTDDDETTAPDREERED-LAPEVPAPPE 319
            DDDDEEG RGGD D+DDDDIEKGDDWEHEEIFTDDDE    D EERED LAPE+PAPPE
Sbjct: 121 NDDDDEEGPRGGDLDMDDDDIEKGDDWEHEEIFTDDDEAVGNDPEEREDLLAPEIPAPPE 180

Query: 320 IKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGEDDDDDDDAEEESGGTPVVP 379
           IKQDEDDE+ E E EGGLSKSGKELKKLL +  GL++SD +DDDD DD EE + GT V  
Sbjct: 181 IKQDEDDEENEEE-EGGLSKSGKELKKLLGKANGLDESDEDDDDDSDDEEETNYGT-VTN 240

Query: 380 SKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSAKGKRK--NGEDVKPSSAPSKKMKTE 439
           SKQK+  KEE   ++P+KP PSG  +GTP  AK +KGKRK  +G+  KPSS+  KK+KTE
Sbjct: 241 SKQKEAAKEEPVDNAPAKPAPSGPPRGTP-PAKPSKGKRKLNDGDSKKPSSSVQKKVKTE 300

Query: 440 TESKST---DEGVSTSKS-TASKAAAASVKTEPASGS----GPVTEEEIRAVLRQRTPVT 499
            + KS+   +   + SKS T +KA  A   + PAS S    GPVTE+EIRAVL ++  VT
Sbjct: 301 NDPKSSLKEERANTVSKSNTPTKAVKAEPASAPASSSSAATGPVTEDEIRAVLMEKKQVT 360

Query: 500 TQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRK 532
           TQDLV++FKARL+++EDKNAFA ILR+IS+IQK  G  ++VVLR+
Sbjct: 361 TQDLVSRFKARLKTKEDKNAFANILRKISKIQKNAGSQNFVVLRE 399

BLAST of Spg038068 vs. TAIR 10
Match: AT4G12610.2 (transcription activators;DNA binding;RNA polymerase II transcription factors;catalytics;transcription initiation factors )

HSP 1 Score: 419.5 bits (1077), Expect = 4.1e-117
Identity = 269/405 (66.42%), Postives = 320/405 (79.01%), Query Frame = 0

Query: 140 LQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRKKTADGYERWMMKAASNGPAAF 199
           +Q KEF AIPAGSWYNFNKVAQYKQLTLEEAEEKMKNR+KTADGY+RWMMKAA+NGPA F
Sbjct: 1   MQNKEFVAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRRKTADGYQRWMMKAANNGPALF 60

Query: 200 GEVEKFDDKDSVGASGRGG-GRRKPT-GDENEGNASDRGEEDEEEEAARKNRLGLNKKGG 259
           GEV+  ++K+S G SG GG GR+K + GDE EGN SDRG+EDEEEEA+RK+RLGLN+K  
Sbjct: 61  GEVD--NEKESGGTSGGGGRGRKKSSGGDEEEGNVSDRGDEDEEEEASRKSRLGLNRK-S 120

Query: 260 GDDDDEEGARGGDHDLDDDDIEKGDDWEHEEIFTDDDETTAPDREERED-LAPEVPAPPE 319
            DDDDEEG RGGD D+DDDDIEKGDDWEHEEIFTDDDE    D EERED LAPE+PAPPE
Sbjct: 121 NDDDDEEGPRGGDLDMDDDDIEKGDDWEHEEIFTDDDEAVGNDPEEREDLLAPEIPAPPE 180

Query: 320 IKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGEDDDDDDDAEEESGGTPVVP 379
           IKQDEDDE+ E E EGGLSKSGKELKKLL +  GL++SD +DDDD DD EE + GT V  
Sbjct: 181 IKQDEDDEENEEE-EGGLSKSGKELKKLLGKANGLDESDEDDDDDSDDEEETNYGT-VTN 240

Query: 380 SKQKDTVKEEAPASSPSKPTPSGSTKGTPSTAKSAKGKRK--NGEDVKPSSAPSKKMKTE 439
           SKQK+  KEE   ++P+KP PSG  +GTP  AK +KGKRK  +G+  KPSS+  KK+KTE
Sbjct: 241 SKQKEAAKEEPVDNAPAKPAPSGPPRGTP-PAKPSKGKRKLNDGDSKKPSSSVQKKVKTE 300

Query: 440 TESKST---DEGVSTSKS-TASKAAAASVKTEPASGS----GPVTEEEIRAVLRQRTPVT 499
            + KS+   +   + SKS T +KA  A   + PAS S    GPVTE+EIRAVL ++  VT
Sbjct: 301 NDPKSSLKEERANTVSKSNTPTKAVKAEPASAPASSSSAATGPVTEDEIRAVLMEKKQVT 360

Query: 500 TQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRK 532
           TQDLV++FKARL+++EDKNAFA ILR+IS+IQK  G  ++VVLR+
Sbjct: 361 TQDLVSRFKARLKTKEDKNAFANILRKISKIQKNAGSQNFVVLRE 399

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888042.15.7e-27093.89transcription initiation factor IIF subunit alpha isoform X1 [Benincasa hispida][more]
XP_022931161.17.5e-27094.09transcription initiation factor IIF subunit alpha-like isoform X1 [Cucurbita mos... [more]
KAG6606328.12.2e-26993.90Transcription initiation factor IIF subunit alpha, partial [Cucurbita argyrosper... [more]
XP_004152016.14.8e-26993.15transcription initiation factor IIF subunit alpha isoform X1 [Cucumis sativus] >... [more]
XP_023532767.15.4e-26893.36transcription initiation factor IIF subunit alpha-like isoform X1 [Cucurbita pep... [more]
Match NameE-valueIdentityDescription
Q9SU257.7e-17767.09Transcription initiation factor IIF subunit alpha OS=Arabidopsis thaliana OX=370... [more]
Q54WV45.6e-1027.08General transcription factor IIF subunit 1 OS=Dictyostelium discoideum OX=44689 ... [more]
Match NameE-valueIdentityDescription
A0A6J1ESX13.6e-27094.09Transcription initiation factor IIF subunit alpha OS=Cucurbita moschata OX=3662 ... [more]
A0A0A0LCA62.3e-26993.15Transcription initiation factor IIF subunit alpha OS=Cucumis sativus OX=3659 GN=... [more]
A0A1S4DWR22.6e-26892.96Transcription initiation factor IIF subunit alpha OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A6J1K1N52.2e-26793.01Transcription initiation factor IIF subunit alpha OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1E3E13.0e-26492.42Transcription initiation factor IIF subunit alpha OS=Momordica charantia OX=3673... [more]
Match NameE-valueIdentityDescription
AT4G12610.14.1e-11766.42transcription activators;DNA binding;RNA polymerase II transcription factors;cat... [more]
AT4G12610.24.1e-11766.42transcription activators;DNA binding;RNA polymerase II transcription factors;cat... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008851Transcription initiation factor IIF, alpha subunitPFAMPF05793TFIIF_alphacoord: 50..530
e-value: 1.9E-139
score: 465.7
IPR008851Transcription initiation factor IIF, alpha subunitPANTHERPTHR13011TFIIF-ALPHAcoord: 1..531
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 452..531
e-value: 3.7E-21
score: 76.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 240..264
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 389..411
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 201..231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 199..474
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 354..369
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 265..300
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 331..353
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 442..456
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 412..441
IPR011039Transcription Factor IIF, Rap30/Rap74, interactionSUPERFAMILY50916Rap30/74 interaction domainscoord: 51..177
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 463..530

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg038068.1Spg038068.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032968 positive regulation of transcription elongation from RNA polymerase II promoter
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0006413 translational initiation
cellular_component GO:0005674 transcription factor TFIIF complex
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0016251 RNA polymerase II general transcription initiation factor activity
molecular_function GO:0001096 TFIIF-class transcription factor complex binding
molecular_function GO:0003743 translation initiation factor activity