Spg038038 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg038038
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionChlorophyllase
Locationscaffold12: 43030907 .. 43034900 (-)
RNA-Seq ExpressionSpg038038
SyntenySpg038038
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAAAGAGATTGCATGAGATGGATATCATATATTATAAAAGAAGGTGTGTGCATAAATAATTAATTTAAGAGAGGAAGAGAAGTTTGCGAAGAAAGTGATCTTAGACAATGTCTGATGATAAGAATAATGCAGATCATCAATTACGAGAGTTTCTTGACATGAACACGAGGAGAAGGAAATGGAATGCAGTGGTCGAGAAGTACGAGGAATACCCAGAATTAGCTCAGAAGCTGAAGCTGAGCCGACAGGGCGACACGGCTCTGCATTTGGCTGTCATCGACAACCAAGAAGAAATAGTTGAAAAGCTTGTGAAGATCATTTGCAGAAACCCAGGAAACGAGCAACTTCTTGAGACTACAAATGAGAGAAACAATAACCCTCTCCATCTCGCCGCCGCGCAGGGGAGCGCCAGAATGTGCCACGCCATTGCTTCAGCCAATGGGCGCTTAGTGGACATGAGAAACGTAGACGACGAAACGCCGCTCTTCTTGGCGGCGGCGTACGGCCACATGGACGCCTTTTATTGCCTTTACTACTTTTGCCGAAACGACTCGGCTCGAATCGCCATCAACTGCAGAGAGAAGAACAATGGAGACACCGTGCTACACCGCGCCCTCAGAGACGACCACTTTGGTGAGCTTTTATAAATTTATAGACCACCTAAATTTGTAGTTTTATTTTTCTTTTTTTAATTTCGATAACATGTAAATAAAAGCGTAGATTCAAAACTCTGACATATTGTGACATGACAGAATTGGCATTTCAGTTAATTCACATGAACAATGATGCTATGAGCTGGGTGAACGAGAAAGGCTTAACCCCACTCCATGTTCTAGCAAGCTACCCAACTTCCTTCAAAAGTGGATCCCACATTAGGGGATGGCAGGAAATCGTCTATTACTGTGAGTATACATTTTAATTAATTGACAATATTTGATACTTGGCTGCAAATCGGTATGTAAAGATCTCTCTACATACTATCATTAAAATGTTGACAAAGATAATTTTTTTTTCCTCATTTTCTATCATTTTCTTTGTTAGTTGTCCAAATTTTAAAACTTTTCAAATTCCAACCTCTTTTTGTTTCTATTCTTGGGTTATTCAAAGGCACATGTGTGGATAAACTAAAGCCACAACCAATAGAAACCCTTAGCAAAGCGTGGACGAAAAGGTTGCCCAAAGCAAAGAGAATTCCTTACTCTTTTCCAGATAACTACGAGACCTGCATTGAATTCTTTATGACGTTGAAGGATGCCATTTTTAAAGGTATATATCCCTCTATTTCTCTCTTTAATTAATTTCTACCAATAAATCGCATTATTCCTTGTAATTTCAGAGTGTAATCTTTTTTTTTTTTTTTTTTTTTGTTATGCAAATATTACAGTCATCACTTTGAATGGACTACTACTTTTCCGCAAGAAGAAAGATGTTGACATGGAAAGGGAAAATTTGGAGACCGTTGAACCTGATCAATCGGGTATAATTTTGGAGACCTTTGTGATATGGGAAATTAAATTAAACATGATGAAACTATAGTATATAATATAATATAATATTCATAATTTTTGTTCGTAGCAGAAAGGGAGGTCCCGATTACAAATTTCCCGAAAAACTATACTACCTGCGTGGATATTTTTCAAATTGTTTTCACGGGCTTGATGATCATCCTTGGGTTCGGTACGTGAAGAAGCTAAGCTAGAATAATCCAATTTTGTGTTTTGAAATATTGAATTTAATTTAGAAAATAGAAATGATGGTGAGCATAGTATATTTTTGGAAATGAAGGATCTAAGGAAATAAAAAAGATAGTAAAGAAGAAACAGAAACACACTTGGTCAGTTCAAGTGATGGAGAAACTTTTTGATCAATTTGGTACACCCGACAAATATGGCGACGATGGAAAAATTCCCGATGGTATGGCAACCGGTCATGAAGATTCTGAAACATTTCCTTACACCTTGGAAGAAAACAAAGTCAATTTCAGTACTAATGTGGTAGACAAGAAACAAAACGACCCCGAGGAGTCCAAGGATGTTCCAGATTCGGCGATGTTATTAGCAGCGAAGAACGGCGTTCTTGAGATGGTGAGGGGACTGTCCCAAAGATTTCCACTGGCGATCCACGATACAAGGAATGACAAGAAGAATGTCCTGCTTTTGGCTGCGGAGTACAGGCAGCCGCAAGTCTACAGGTTTTTACACAACAAAAAGGATGACTTAGAAAGTCTGTTTCGAGCTGTCGATGTCGATGGAAACGGCGCCTTGCATCTCGCAGCCGCCGCCTCAAAGTCTATGGTTTGGCGCATCACCGGAGATGCACTGCAGATGCAATGGGAAGTTAAATGGTATAAGGTAATTAATTCATGAACAAAACTTGGCTGCATACCCGGTATGTAGGGATCTGCCTACATACCCCATCAAAATCTTGACAAGTAAGAAAGAGGAAGGGAGAAAATGAGAATTTTTTGATATCTCTCTTAGTTGTCTAACGTTTTAATTGTGGTATGCAGCGAGATCCCTAGTATGCAGTCAAGTTTTTTTCTTAATTCATTATTATTTAACTTTTCCAATTTTGTGATTGTTTTTTAAATTACTTTTAAAATATCTAAACATGTATATATATATTACAGTTCATTGAGGAGTCGATGCCACTCCATTTCTTCACCCACTATAACAAGGAAGAAAAAACTGCAAGTACAATCTTCCATGAAACCCACATGGACATGGTGAAGAACGGCGGCGACTGGCTTACTCAAACCTCCAAGTCATGCTCGGTGGTGGGCACCCTGATTGTAACAGTGGCTTTTACTTCCGCCACCACTATCCCCGGCGGCTATGACACGGAGGGCGTCCCATTCCTTGAAAAGGAGCAAAGCTTTTTCATCTTCGCCGTGTCTTCCCTAATTGCCCTCTGCCTCTCTTCAGCCTCCGTCACCATGTTTCTTGCCATCTTGACCTCCAGGTTCGAAGCCAAAGACTTCGGTTCCGACTTGCCTTGGAAACTCTTCATAGGCCTTTCCTCTCTTTTCTTTTCCATAGTCTCCACCTTGATTTCCTTCTGCGCCGGCCACTACTTCCTCATCGACCACCGCCTTCGCAGCGTCGCCCTTCTTCTCTACACACTCACTTTTCTCCCCGTCGCATTCATCTTCCTCATCTCCAAGCTTCCTCTCTACATCGACGTCGTGCGCGCCCTTGTCACAACCGTGCCTAGAAGGAGCGCCGACGTCGTCCCCCCCGTCGACCGGAAACCTTCTAAACCTCTCCAAAAAGGAAAATTTGAGGTTTCTTCGATCAGGGCTGAGATCGACTCGGACGAAATAATGCCGTTGGTGATCTTCACACCATCGACTCAAGATTCATATCCTGTGATCTTGTTTCTTCCTGGCTGCGTTGAATCCTTCCACTCCGATTTCCTCCGGCTTGTAGCTTCACACGGCTTCGTTATTGTTGCCCCAGAGGTATGTACTTTCATTCAATCACTTAAAATAATAATGTAGATCATTATTGGTTAATTTTGAATTAACCCATGTCCATAATTTTTAGTTGGAAGCGAAGAACACAGAGTCAACATCACAAGTGAAAAGTTCGGATCGAGAAATGGTGGAAGAAAAGCTATCAAAACTGTCAAAGACGACTCAGAGGGAAGGAGAAGAAACACCATTGTTGTCCTTAGCTTTAGCGAGCGGCGAGAGACCTTCCCATCCATTTTCAGAAGTGATTGGGTTCGACCCAATGCCTGGAACCAAATTACACATCCCAAACTCTCAAATTATTCAGACCCATTCCACTCAATCCTCCCACACATCCGCACCAATTCTTCAAAGTGAATTTGTAATTTCCAACTTATGCGGACATGTCAAATTGTTGGATTAATCCAACACTAATTTCCAACTTACTAATTATCAAGAATTTTAACTGGTTTTTTTTTTCTAGAATGCATGCGAAATGATTTTGTATGTTTCTATAGATGTTTAATATGTATTCTGTGCG

mRNA sequence

ATGTCTGATGATAAGAATAATGCAGATCATCAATTACGAGAGTTTCTTGACATGAACACGAGGAGAAGGAAATGGAATGCAGTGGTCGAGAAGTACGAGGAATACCCAGAATTAGCTCAGAAGCTGAAGCTGAGCCGACAGGGCGACACGGCTCTGCATTTGGCTGTCATCGACAACCAAGAAGAAATAGTTGAAAAGCTTGTGAAGATCATTTGCAGAAACCCAGGAAACGAGCAACTTCTTGAGACTACAAATGAGAGAAACAATAACCCTCTCCATCTCGCCGCCGCGCAGGGGAGCGCCAGAATGTGCCACGCCATTGCTTCAGCCAATGGGCGCTTAGTGGACATGAGAAACGTAGACGACGAAACGCCGCTCTTCTTGGCGGCGGCGTACGGCCACATGGACGCCTTTTATTGCCTTTACTACTTTTGCCGAAACGACTCGGCTCGAATCGCCATCAACTGCAGAGAGAAGAACAATGGAGACACCGTGCTACACCGCGCCCTCAGAGACGACCACTTTGAATTGGCATTTCAGTTAATTCACATGAACAATGATGCTATGAGCTGGGTGAACGAGAAAGGCTTAACCCCACTCCATGTTCTAGCAAGCTACCCAACTTCCTTCAAAAGTGGATCCCACATTAGGGGATGGCAGGAAATCGTCTATTACTGCACATGTGTGGATAAACTAAAGCCACAACCAATAGAAACCCTTAGCAAAGCGTGGACGAAAAGGTTGCCCAAAGCAAAGAGAATTCCTTACTCTTTTCCAGATAACTACGAGACCTGCATTGAATTCTTTATGACGTTGAAGGATGCCATTTTTAAAGGATCTAAGGAAATAAAAAAGATAGTAAAGAAGAAACAGAAACACACTTGGTCAGTTCAAGTGATGGAGAAACTTTTTGATCAATTTGGTACACCCGACAAATATGGCGACGATGGAAAAATTCCCGATGGTATGGCAACCGGTCATGAAGATTCTGAAACATTTCCTTACACCTTGGAAGAAAACAAAGTCAATTTCAGTACTAATGTGGTAGACAAGAAACAAAACGACCCCGAGGAGTCCAAGGATGTTCCAGATTCGGCGATGTTATTAGCAGCGAAGAACGGCGTTCTTGAGATGGTGAGGGGACTGTCCCAAAGATTTCCACTGGCGATCCACGATACAAGGAATGACAAGAAGAATGTCCTGCTTTTGGCTGCGGAGTACAGGCAGCCGCAAGTCTACAGGTTTTTACACAACAAAAAGGATGACTTAGAAAGTCTGTTTCGAGCTGTCGATGTCGATGGAAACGGCGCCTTGCATCTCGCAGCCGCCGCCTCAAAGTCTATGGTTTGGCGCATCACCGGAGATGCACTGCAGATGCAATGGGAAGTTAAATGGTATAAGTTCATTGAGGAGTCGATGCCACTCCATTTCTTCACCCACTATAACAAGGAAGAAAAAACTGCAAGTACAATCTTCCATGAAACCCACATGGACATGGTGAAGAACGGCGGCGACTGGCTTACTCAAACCTCCAAGTCATGCTCGGTGGTGGGCACCCTGATTGTAACAGTGGCTTTTACTTCCGCCACCACTATCCCCGGCGGCTATGACACGGAGGGCGTCCCATTCCTTGAAAAGGAGCAAAGCTTTTTCATCTTCGCCGTGTCTTCCCTAATTGCCCTCTGCCTCTCTTCAGCCTCCGTCACCATGTTTCTTGCCATCTTGACCTCCAGGTTCGAAGCCAAAGACTTCGGTTCCGACTTGCCTTGGAAACTCTTCATAGGCCTTTCCTCTCTTTTCTTTTCCATAGTCTCCACCTTGATTTCCTTCTGCGCCGGCCACTACTTCCTCATCGACCACCGCCTTCGCAGCGTCGCCCTTCTTCTCTACACACTCACTTTTCTCCCCGTCGCATTCATCTTCCTCATCTCCAAGCTTCCTCTCTACATCGACGTCGTGCGCGCCCTTGTCACAACCGTGCCTAGAAGGAGCGCCGACGTCGTCCCCCCCGTCGACCGGAAACCTTCTAAACCTCTCCAAAAAGGAAAATTTGAGGTTTCTTCGATCAGGGCTGAGATCGACTCGGACGAAATAATGCCGTTGGTGATCTTCACACCATCGACTCAAGATTCATATCCTGTGATCTTGTTTCTTCCTGGCTGCGTTGAATCCTTCCACTCCGATTTCCTCCGGCTTGTAGCTTCACACGGCTTCGTTATTGTTGCCCCAGAGTTGGAAGCGAAGAACACAGAGTCAACATCACAAGTGAAAAGTTCGGATCGAGAAATGGTGGAAGAAAAGCTATCAAAACTGTCAAAGACGACTCAGAGGGAAGGAGAAGAAACACCATTGTTGTCCTTAGCTTTAGCGAGCGGCGAGAGACCTTCCCATCCATTTTCAGAAGTGATTGGGTTCGACCCAATGCCTGGAACCAAATTACACATCCCAAACTCTCAAATTATTCAGACCCATTCCACTCAATCCTCCCACACATCCGCACCAATTCTTCAAAGTGAATTTGTAATTTCCAACTTATGCGGACATGTCAAATTGTTGGATTAA

Coding sequence (CDS)

ATGTCTGATGATAAGAATAATGCAGATCATCAATTACGAGAGTTTCTTGACATGAACACGAGGAGAAGGAAATGGAATGCAGTGGTCGAGAAGTACGAGGAATACCCAGAATTAGCTCAGAAGCTGAAGCTGAGCCGACAGGGCGACACGGCTCTGCATTTGGCTGTCATCGACAACCAAGAAGAAATAGTTGAAAAGCTTGTGAAGATCATTTGCAGAAACCCAGGAAACGAGCAACTTCTTGAGACTACAAATGAGAGAAACAATAACCCTCTCCATCTCGCCGCCGCGCAGGGGAGCGCCAGAATGTGCCACGCCATTGCTTCAGCCAATGGGCGCTTAGTGGACATGAGAAACGTAGACGACGAAACGCCGCTCTTCTTGGCGGCGGCGTACGGCCACATGGACGCCTTTTATTGCCTTTACTACTTTTGCCGAAACGACTCGGCTCGAATCGCCATCAACTGCAGAGAGAAGAACAATGGAGACACCGTGCTACACCGCGCCCTCAGAGACGACCACTTTGAATTGGCATTTCAGTTAATTCACATGAACAATGATGCTATGAGCTGGGTGAACGAGAAAGGCTTAACCCCACTCCATGTTCTAGCAAGCTACCCAACTTCCTTCAAAAGTGGATCCCACATTAGGGGATGGCAGGAAATCGTCTATTACTGCACATGTGTGGATAAACTAAAGCCACAACCAATAGAAACCCTTAGCAAAGCGTGGACGAAAAGGTTGCCCAAAGCAAAGAGAATTCCTTACTCTTTTCCAGATAACTACGAGACCTGCATTGAATTCTTTATGACGTTGAAGGATGCCATTTTTAAAGGATCTAAGGAAATAAAAAAGATAGTAAAGAAGAAACAGAAACACACTTGGTCAGTTCAAGTGATGGAGAAACTTTTTGATCAATTTGGTACACCCGACAAATATGGCGACGATGGAAAAATTCCCGATGGTATGGCAACCGGTCATGAAGATTCTGAAACATTTCCTTACACCTTGGAAGAAAACAAAGTCAATTTCAGTACTAATGTGGTAGACAAGAAACAAAACGACCCCGAGGAGTCCAAGGATGTTCCAGATTCGGCGATGTTATTAGCAGCGAAGAACGGCGTTCTTGAGATGGTGAGGGGACTGTCCCAAAGATTTCCACTGGCGATCCACGATACAAGGAATGACAAGAAGAATGTCCTGCTTTTGGCTGCGGAGTACAGGCAGCCGCAAGTCTACAGGTTTTTACACAACAAAAAGGATGACTTAGAAAGTCTGTTTCGAGCTGTCGATGTCGATGGAAACGGCGCCTTGCATCTCGCAGCCGCCGCCTCAAAGTCTATGGTTTGGCGCATCACCGGAGATGCACTGCAGATGCAATGGGAAGTTAAATGGTATAAGTTCATTGAGGAGTCGATGCCACTCCATTTCTTCACCCACTATAACAAGGAAGAAAAAACTGCAAGTACAATCTTCCATGAAACCCACATGGACATGGTGAAGAACGGCGGCGACTGGCTTACTCAAACCTCCAAGTCATGCTCGGTGGTGGGCACCCTGATTGTAACAGTGGCTTTTACTTCCGCCACCACTATCCCCGGCGGCTATGACACGGAGGGCGTCCCATTCCTTGAAAAGGAGCAAAGCTTTTTCATCTTCGCCGTGTCTTCCCTAATTGCCCTCTGCCTCTCTTCAGCCTCCGTCACCATGTTTCTTGCCATCTTGACCTCCAGGTTCGAAGCCAAAGACTTCGGTTCCGACTTGCCTTGGAAACTCTTCATAGGCCTTTCCTCTCTTTTCTTTTCCATAGTCTCCACCTTGATTTCCTTCTGCGCCGGCCACTACTTCCTCATCGACCACCGCCTTCGCAGCGTCGCCCTTCTTCTCTACACACTCACTTTTCTCCCCGTCGCATTCATCTTCCTCATCTCCAAGCTTCCTCTCTACATCGACGTCGTGCGCGCCCTTGTCACAACCGTGCCTAGAAGGAGCGCCGACGTCGTCCCCCCCGTCGACCGGAAACCTTCTAAACCTCTCCAAAAAGGAAAATTTGAGGTTTCTTCGATCAGGGCTGAGATCGACTCGGACGAAATAATGCCGTTGGTGATCTTCACACCATCGACTCAAGATTCATATCCTGTGATCTTGTTTCTTCCTGGCTGCGTTGAATCCTTCCACTCCGATTTCCTCCGGCTTGTAGCTTCACACGGCTTCGTTATTGTTGCCCCAGAGTTGGAAGCGAAGAACACAGAGTCAACATCACAAGTGAAAAGTTCGGATCGAGAAATGGTGGAAGAAAAGCTATCAAAACTGTCAAAGACGACTCAGAGGGAAGGAGAAGAAACACCATTGTTGTCCTTAGCTTTAGCGAGCGGCGAGAGACCTTCCCATCCATTTTCAGAAGTGATTGGGTTCGACCCAATGCCTGGAACCAAATTACACATCCCAAACTCTCAAATTATTCAGACCCATTCCACTCAATCCTCCCACACATCCGCACCAATTCTTCAAAGTGAATTTGTAATTTCCAACTTATGCGGACATGTCAAATTGTTGGATTAA

Protein sequence

MSDDKNNADHQLREFLDMNTRRRKWNAVVEKYEEYPELAQKLKLSRQGDTALHLAVIDNQEEIVEKLVKIICRNPGNEQLLETTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMRNVDDETPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELAFQLIHMNNDAMSWVNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIETLSKAWTKRLPKAKRIPYSFPDNYETCIEFFMTLKDAIFKGSKEIKKIVKKKQKHTWSVQVMEKLFDQFGTPDKYGDDGKIPDGMATGHEDSETFPYTLEENKVNFSTNVVDKKQNDPEESKDVPDSAMLLAAKNGVLEMVRGLSQRFPLAIHDTRNDKKNVLLLAAEYRQPQVYRFLHNKKDDLESLFRAVDVDGNGALHLAAAASKSMVWRITGDALQMQWEVKWYKFIEESMPLHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTSKSCSVVGTLIVTVAFTSATTIPGGYDTEGVPFLEKEQSFFIFAVSSLIALCLSSASVTMFLAILTSRFEAKDFGSDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVALLLYTLTFLPVAFIFLISKLPLYIDVVRALVTTVPRRSADVVPPVDRKPSKPLQKGKFEVSSIRAEIDSDEIMPLVIFTPSTQDSYPVILFLPGCVESFHSDFLRLVASHGFVIVAPELEAKNTESTSQVKSSDREMVEEKLSKLSKTTQREGEETPLLSLALASGERPSHPFSEVIGFDPMPGTKLHIPNSQIIQTHSTQSSHTSAPILQSEFVISNLCGHVKLLD
Homology
BLAST of Spg038038 vs. NCBI nr
Match: XP_022995621.1 (uncharacterized protein LOC111491104 isoform X2 [Cucurbita maxima])

HSP 1 Score: 890.6 bits (2300), Expect = 1.1e-254
Identity = 510/945 (53.97%), Postives = 632/945 (66.88%), Query Frame = 0

Query: 12  LREFLDMNTRRRKWNAVVEKYEEYPELAQKLKLSRQGDTALHLAVIDNQEEIVEKLVKII 71
           LR+FL  NT+R KW  V++KYEEYPE AQ+LKL+R GDTALHLAV+DN+EE+V+KLV  I
Sbjct: 34  LRDFLYTNTKRGKWEEVIKKYEEYPE-AQELKLTRNGDTALHLAVLDNREEVVQKLVNRI 93

Query: 72  CRNPGNEQLLETTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMRNVDDETPLFLAAA 131
                 ++LL+TTN+R   PLHLAA  GSA MC+AIASA+  LVD+RN  DETPL+LAAA
Sbjct: 94  KHTSKYDELLKTTNDREETPLHLAAQMGSATMCNAIASAHDELVDLRNKVDETPLYLAAA 153

Query: 132 YGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELAFQLIHMNNDAMSW 191
            G+ DAF+CLY+FCRN+++RI  NCR  +NGDTVLH ALR+DHF+LAFQ++H+NN+AM W
Sbjct: 154 SGNRDAFFCLYHFCRNNASRITANCRLTSNGDTVLHSALRNDHFDLAFQILHLNNEAMHW 213

Query: 192 VNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIETLSKAWTKRLPKA 251
           V E G+TPLHVLAS PT+FKSGS IRGW+ I YYCT VD+LKPQPI++L + W  R+   
Sbjct: 214 VTETGITPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSLIRDWIDRMSNP 273

Query: 252 KRIPYSFPDNYETCIEFFMTLKDAIFK--------------------------------- 311
                 FP NY TCI+FF  + D   K                                 
Sbjct: 274 NTSTPCFPANYGTCIDFFTWVWDGFLKGSGLKRICHDNENDESKKDTQVVRNIMVEGGES 333

Query: 312 -----------------------------------------------------GSKEIKK 371
                                                                GS E KK
Sbjct: 334 LETDESHQRLDTELLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAIMISLGLGSAEFKK 393

Query: 372 IVKKKQKHTWSVQVMEKLFDQFGTPDKYGDDGKIP-DGMATGHEDSE-TFPYTLEENKVN 431
           I +KK+KHTWSVQVMEKL  ++  PD+Y  +G  P D  +   + +E T PY+L   +V 
Sbjct: 394 IRRKKEKHTWSVQVMEKLL-EYAPPDEYECNGGTPMDSTSQTPDQAEVTLPYSLVAGEVR 453

Query: 432 FSTNVVDKKQNDPEESKDVPDSAMLLAAKNGVLEMVRGLSQRFPLAIHDTRNDKKNVLLL 491
            S N ++ K  + E+ K+V ++AMLLAAKNGV+E+V+G+  RFPL+I D R DKKNV+LL
Sbjct: 454 LS-NSIESKPKEAEKPKNVQETAMLLAAKNGVIEIVKGMFHRFPLSICDARKDKKNVVLL 513

Query: 492 AAEYRQPQVYRFLHNKKDDLESLFRAVDVDGNGALHLAAAASKSMVWRITGDALQMQWEV 551
           AAEY QP VYRFL + K   E+LFRAVD +GN ALHLAAAASKSM+WRITG ALQMQWE+
Sbjct: 514 AAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIWRITGAALQMQWEI 573

Query: 552 KWYKFIEESMPLHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTSKSCSVVGTLIVT 611
           KWYKF+EES+PL+FF HYNKE K A+ IFHETHMD+VK  G+WLT+TSKSCSVVGTLIVT
Sbjct: 574 KWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVKKSGEWLTKTSKSCSVVGTLIVT 633

Query: 612 VAFTSATTIPGGYDT-EGVPFLEKEQSFFIFAVSSLIALCLSSASVTMFLAILTSRFEAK 671
           VAFTS  +IPGG++  +G PFL+  ++FF FA+ SLIALCLSS SVTMFLAILT RF+A 
Sbjct: 634 VAFTSVASIPGGFNPHDGSPFLKDRKAFFTFALFSLIALCLSSTSVTMFLAILTYRFDAN 693

Query: 672 DFGSDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVALLLYTLTFLPVAFIFL 731
           DF ++LPWKLFIG SSLF SI+S L+SFCAGHYFL+   +   A LLYT+  +PVA IF+
Sbjct: 694 DFRTNLPWKLFIGFSSLFGSIISMLVSFCAGHYFLMHQHIPHHAALLYTIVLVPVALIFI 753

Query: 732 ISKLPLYIDVVRALVTTVPRRSADVV---PPVDRKPS-KPLQKGKFEVSSIRAEIDSDEI 791
           ISKLPLYIDVV+A+   VP RSA VV   P     PS KP QKGKFEV+SI  E DSD  
Sbjct: 754 ISKLPLYIDVVQAIFKIVPNRSAHVVLSDPLPPHTPSIKPFQKGKFEVTSIPVE-DSDAF 813

Query: 792 M---PLVIFTPSTQDSYPVILFLPGCVESFHSDFLRLVASHGFVIVAP-ELEAKNTES-- 851
               PL+I TP+ Q SYP++ FLPGC E  +S FL+L+AS G VIV P ++ A+ T S  
Sbjct: 814 SPSNPLLILTPTAQGSYPLLFFLPGCAEYDYSHFLQLIASQGLVIVWPLQMSAEATRSEM 873

BLAST of Spg038038 vs. NCBI nr
Match: XP_022995622.1 (uncharacterized protein LOC111491104 isoform X3 [Cucurbita maxima])

HSP 1 Score: 889.0 bits (2296), Expect = 3.2e-254
Identity = 511/944 (54.13%), Postives = 632/944 (66.95%), Query Frame = 0

Query: 12  LREFLDMNTRRRKWNAVVEKYEEYPELAQKLKLSRQGDTALHLAVIDNQEEIVEKLVKII 71
           LR+FL  NT+R KW  V++KYEEYPE AQ+LKL+R GDTALHLAV+DN+EE+V+KLV  I
Sbjct: 34  LRDFLYTNTKRGKWEEVIKKYEEYPE-AQELKLTRNGDTALHLAVLDNREEVVQKLVNRI 93

Query: 72  CRNPGNEQLLETTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMRNVDDETPLFLAAA 131
                 ++LL+TTN+R   PLHLAA  GSA MC+AIASA+  LVD+RN  DETPL+LAAA
Sbjct: 94  KHTSKYDELLKTTNDREETPLHLAAQMGSATMCNAIASAHDELVDLRNKVDETPLYLAAA 153

Query: 132 YGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELAFQLIHMNNDAMSW 191
            G+ DAF+CLY+FCRN+++RI  NCR  +NGDTVLH ALR+DHF+LAFQ++H+NN+AM W
Sbjct: 154 SGNRDAFFCLYHFCRNNASRITANCRLTSNGDTVLHSALRNDHFDLAFQILHLNNEAMHW 213

Query: 192 VNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIETLSKAWTKRLPKA 251
           V E G+TPLHVLAS PT+FKSGS IRGW+ I YYCT VD+LKPQPI++L + W  R+   
Sbjct: 214 VTETGITPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSLIRDWIDRMSNP 273

Query: 252 KRIPYSFPDNYETCIEFFMTLKDAIFK--------------------------------- 311
                 FP NY TCI+FF  + D   K                                 
Sbjct: 274 NTSTPCFPANYGTCIDFFTWVWDGFLKGSGLKRICHDNENDESKKDTQVVRNIMVEGGES 333

Query: 312 -----------------------------------------------------GSKEIKK 371
                                                                GS E KK
Sbjct: 334 LETDESHQRLDTELLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAIMISLGLGSAEFKK 393

Query: 372 IVKKKQKHTWSVQVMEKLFDQFGTPDKYGDDGKIP-DGMATGHEDSE-TFPYTLEENKVN 431
           I +KK+KHTWSVQVMEKL  ++  PD+Y  +G  P D  +   + +E T PY+L   +V 
Sbjct: 394 IRRKKEKHTWSVQVMEKLL-EYAPPDEYECNGGTPMDSTSQTPDQAEVTLPYSLVAGEVR 453

Query: 432 FSTNVVDKKQNDPEESKDV--PDSAMLLAAKNGVLEMVRGLSQRFPLAIHDTRNDKKNVL 491
            S N ++ K  + E+ K+V  P++AMLLAAKNGV+E+V+G+  RFPL+I D R DKKNV+
Sbjct: 454 LS-NSIESKPKEAEKPKNVQAPETAMLLAAKNGVIEIVKGMFHRFPLSICDARKDKKNVV 513

Query: 492 LLAAEYRQPQVYRFLHNKKDDLESLFRAVDVDGNGALHLAAAASKSMVWRITGDALQMQW 551
           LLAAEY QP VYRFL + K   E+LFRAVD +GN ALHLAAAASKSM+WRITG ALQMQW
Sbjct: 514 LLAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIWRITGAALQMQW 573

Query: 552 EVKWYKFIEESMPLHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTSKSCSVVGTLI 611
           E+KWYKF+EES+PL+FF HYNKE K A+ IFHETHMD+VK  G+WLT+TSKSCSVVGTLI
Sbjct: 574 EIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVKKSGEWLTKTSKSCSVVGTLI 633

Query: 612 VTVAFTSATTIPGGYDT-EGVPFLEKEQSFFIFAVSSLIALCLSSASVTMFLAILTSRFE 671
           VTVAFTS  +IPGG++  +G PFL+  ++FF FA+ SLIALCLSS SVTMFLAILT RF+
Sbjct: 634 VTVAFTSVASIPGGFNPHDGSPFLKDRKAFFTFALFSLIALCLSSTSVTMFLAILTYRFD 693

Query: 672 AKDFGSDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVALLLYTLTFLPVAFI 731
           A DF ++LPWKLFIG SSLF SI+S L+SFCAGHYFL+   +   A LLYT+  +PVA I
Sbjct: 694 ANDFRTNLPWKLFIGFSSLFGSIISMLVSFCAGHYFLMHQHIPHHAALLYTIVLVPVALI 753

Query: 732 FLISKLPLYIDVVRALVTTVPRRSADVV---PPVDRKPS-KPLQKGKFEVSSIRAEIDSD 791
           F+ISKLPLYIDVV+A+   VP RSA VV   P     PS KP QKGKFEV+SI  E DSD
Sbjct: 754 FIISKLPLYIDVVQAIFKIVPNRSAHVVLSDPLPPHTPSIKPFQKGKFEVTSIPVE-DSD 813

Query: 792 EIM---PLVIFTPSTQDSYPVILFLPGCVESFHSDFLRLVASHGFVIVAPELEAKNTE-- 851
                 PL+I TP+ Q SYP++ FLPGC E  +S FL+L+AS G VIV P L+A  +E  
Sbjct: 814 AFSPSNPLLILTPTAQGSYPLLFFLPGCAEYDYSHFLQLIASQGLVIVWP-LQATRSEMN 873

BLAST of Spg038038 vs. NCBI nr
Match: XP_022995620.1 (uncharacterized protein LOC111491104 isoform X1 [Cucurbita maxima])

HSP 1 Score: 888.6 bits (2295), Expect = 4.1e-254
Identity = 511/947 (53.96%), Postives = 633/947 (66.84%), Query Frame = 0

Query: 12  LREFLDMNTRRRKWNAVVEKYEEYPELAQKLKLSRQGDTALHLAVIDNQEEIVEKLVKII 71
           LR+FL  NT+R KW  V++KYEEYPE AQ+LKL+R GDTALHLAV+DN+EE+V+KLV  I
Sbjct: 34  LRDFLYTNTKRGKWEEVIKKYEEYPE-AQELKLTRNGDTALHLAVLDNREEVVQKLVNRI 93

Query: 72  CRNPGNEQLLETTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMRNVDDETPLFLAAA 131
                 ++LL+TTN+R   PLHLAA  GSA MC+AIASA+  LVD+RN  DETPL+LAAA
Sbjct: 94  KHTSKYDELLKTTNDREETPLHLAAQMGSATMCNAIASAHDELVDLRNKVDETPLYLAAA 153

Query: 132 YGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELAFQLIHMNNDAMSW 191
            G+ DAF+CLY+FCRN+++RI  NCR  +NGDTVLH ALR+DHF+LAFQ++H+NN+AM W
Sbjct: 154 SGNRDAFFCLYHFCRNNASRITANCRLTSNGDTVLHSALRNDHFDLAFQILHLNNEAMHW 213

Query: 192 VNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIETLSKAWTKRLPKA 251
           V E G+TPLHVLAS PT+FKSGS IRGW+ I YYCT VD+LKPQPI++L + W  R+   
Sbjct: 214 VTETGITPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSLIRDWIDRMSNP 273

Query: 252 KRIPYSFPDNYETCIEFFMTLKDAIFK--------------------------------- 311
                 FP NY TCI+FF  + D   K                                 
Sbjct: 274 NTSTPCFPANYGTCIDFFTWVWDGFLKGSGLKRICHDNENDESKKDTQVVRNIMVEGGES 333

Query: 312 -----------------------------------------------------GSKEIKK 371
                                                                GS E KK
Sbjct: 334 LETDESHQRLDTELLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAIMISLGLGSAEFKK 393

Query: 372 IVKKKQKHTWSVQVMEKLFDQFGTPDKYGDDGKIP-DGMATGHEDSE-TFPYTLEENKVN 431
           I +KK+KHTWSVQVMEKL  ++  PD+Y  +G  P D  +   + +E T PY+L   +V 
Sbjct: 394 IRRKKEKHTWSVQVMEKLL-EYAPPDEYECNGGTPMDSTSQTPDQAEVTLPYSLVAGEVR 453

Query: 432 FSTNVVDKKQNDPEESKDV--PDSAMLLAAKNGVLEMVRGLSQRFPLAIHDTRNDKKNVL 491
            S N ++ K  + E+ K+V  P++AMLLAAKNGV+E+V+G+  RFPL+I D R DKKNV+
Sbjct: 454 LS-NSIESKPKEAEKPKNVQAPETAMLLAAKNGVIEIVKGMFHRFPLSICDARKDKKNVV 513

Query: 492 LLAAEYRQPQVYRFLHNKKDDLESLFRAVDVDGNGALHLAAAASKSMVWRITGDALQMQW 551
           LLAAEY QP VYRFL + K   E+LFRAVD +GN ALHLAAAASKSM+WRITG ALQMQW
Sbjct: 514 LLAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIWRITGAALQMQW 573

Query: 552 EVKWYKFIEESMPLHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTSKSCSVVGTLI 611
           E+KWYKF+EES+PL+FF HYNKE K A+ IFHETHMD+VK  G+WLT+TSKSCSVVGTLI
Sbjct: 574 EIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVKKSGEWLTKTSKSCSVVGTLI 633

Query: 612 VTVAFTSATTIPGGYDT-EGVPFLEKEQSFFIFAVSSLIALCLSSASVTMFLAILTSRFE 671
           VTVAFTS  +IPGG++  +G PFL+  ++FF FA+ SLIALCLSS SVTMFLAILT RF+
Sbjct: 634 VTVAFTSVASIPGGFNPHDGSPFLKDRKAFFTFALFSLIALCLSSTSVTMFLAILTYRFD 693

Query: 672 AKDFGSDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVALLLYTLTFLPVAFI 731
           A DF ++LPWKLFIG SSLF SI+S L+SFCAGHYFL+   +   A LLYT+  +PVA I
Sbjct: 694 ANDFRTNLPWKLFIGFSSLFGSIISMLVSFCAGHYFLMHQHIPHHAALLYTIVLVPVALI 753

Query: 732 FLISKLPLYIDVVRALVTTVPRRSADVV---PPVDRKPS-KPLQKGKFEVSSIRAEIDSD 791
           F+ISKLPLYIDVV+A+   VP RSA VV   P     PS KP QKGKFEV+SI  E DSD
Sbjct: 754 FIISKLPLYIDVVQAIFKIVPNRSAHVVLSDPLPPHTPSIKPFQKGKFEVTSIPVE-DSD 813

Query: 792 EIM---PLVIFTPSTQDSYPVILFLPGCVESFHSDFLRLVASHGFVIVAP-ELEAKNTES 851
                 PL+I TP+ Q SYP++ FLPGC E  +S FL+L+AS G VIV P ++ A+ T S
Sbjct: 814 AFSPSNPLLILTPTAQGSYPLLFFLPGCAEYDYSHFLQLIASQGLVIVWPLQMSAEATRS 873

BLAST of Spg038038 vs. NCBI nr
Match: XP_023534305.1 (uncharacterized protein LOC111795904 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 862.8 bits (2228), Expect = 2.4e-246
Identity = 493/957 (51.52%), Postives = 636/957 (66.46%), Query Frame = 0

Query: 3   DDKNNADHQ--LREFLDMNTRRRKWNAVVEKYEEYPELAQKLKLSRQGDTALHLAVIDNQ 62
           ++  N +H+  LR+FL +NT+R +W  V++KYE++PE A +LKL+R GDTALHLAV+DN+
Sbjct: 9   EESMNEEHKATLRDFLYINTKRTEWEKVIKKYEKHPE-ALELKLTRNGDTALHLAVLDNR 68

Query: 63  EEIVEKLVKIICRNPGNEQLLETTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMRNV 122
           E++V+KLV  I ++   ++LLETTN+R  NPLHLAA  GSA MC+AIASA  +LV  RN 
Sbjct: 69  EDVVQKLVNRI-KDSKCDKLLETTNDRKENPLHLAAQMGSATMCNAIASACDKLVHERNK 128

Query: 123 DDETPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELAFQ 182
            DETPL+LAAA+G+ DAF+CLY+FCRN ++RI  NCR  +NGDTVLH ALR++HF+LAFQ
Sbjct: 129 MDETPLYLAAAFGNRDAFFCLYHFCRNVASRITANCRLTSNGDTVLHSALRNEHFDLAFQ 188

Query: 183 LIHMNNDAMSWVNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIETL 242
           ++H+NN+AM WV + G+TPLHVLAS PT+FKSGS IRGW+ I YYCT VD+LKPQPI++L
Sbjct: 189 ILHLNNEAMHWVTKDGVTPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSL 248

Query: 243 SKAWTKRLPKAKRIPYSFPDNYETCIEFFMTLKDAIFK---------------------- 302
            + W  R+         FP NYETCI+FF  + D   K                      
Sbjct: 249 IRDWIDRMSNPNPSTPCFPANYETCIDFFTWVWDGFLKGSGLKRICHDNKNDETKKDTDD 308

Query: 303 ------------------------------------------------------------ 362
                                                                       
Sbjct: 309 AGRNIMVEGGESLEADEPHQRLDTQLLKAHGLAKEASMTNVPRNYNTCIHFFQIVFSAIL 368

Query: 363 -----GSKEIKKIVKKKQKHTWSVQVMEKLFDQFGTPDKYGDDGKIP--DGMATGHEDSE 422
                GS E KKI ++K+KHTWSVQVMEKL  ++  PD+Y  +G IP      T  +  +
Sbjct: 369 ISLGLGSAEFKKIRRQKEKHTWSVQVMEKLL-EYAAPDEYDCNGGIPMDSTSRTPDQAGD 428

Query: 423 TFPYTLEENKVNFSTNVVDKKQNDPEESKDVPDSAMLLAAKNGVLEMVRGLSQRFPLAIH 482
           T PY+ +++ V FS + ++ K  + E+ KD  ++ MLLAAKNGV+E+V+G+ +RFPL+I+
Sbjct: 429 TLPYSFKDDDVLFSVH-IESKPTEAEKPKDFQETPMLLAAKNGVIEIVKGMFRRFPLSIY 488

Query: 483 DTRNDKKNVLLLAAEYRQPQVYRFLHNKKDDLESLFRAVDVDGNGALHLAAAASKSMVWR 542
           D   DKKNV+LLAAEY QP+VYRFL +     E+LFRAVD +GN ALHLAAAASKSM+WR
Sbjct: 489 DAGKDKKNVVLLAAEYGQPEVYRFLLSPGVYKENLFRAVDDNGNSALHLAAAASKSMIWR 548

Query: 543 ITGDALQMQWEVKWYKFIEESMPLHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTS 602
           ITG ALQMQWE+KWYKF+EES+PL+FF HYNKE K A+ IFHETHMD+V+  GDWLT+TS
Sbjct: 549 ITGAALQMQWEIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVQKSGDWLTKTS 608

Query: 603 KSCSVVGTLIVTVAFTSATTIPGGYD-TEGVPFLEKEQSFFIFAVSSLIALCLSSASVTM 662
           KSCSVVG LIVTVAFTS  +IPGG++  +G PFL+  ++FF FA+ SLIALCLSS SVTM
Sbjct: 609 KSCSVVGALIVTVAFTSVASIPGGFNPRDGSPFLQDREAFFTFALFSLIALCLSSTSVTM 668

Query: 663 FLAILTSRFEAKDFGSDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVALLLY 722
           FLAILT RF+A DF ++LPWKLFIG SSLF SI+S LISFCAGHYFL+   +   A LLY
Sbjct: 669 FLAILTHRFDANDFRTNLPWKLFIGFSSLFGSIISMLISFCAGHYFLMHRHIPHHAALLY 728

Query: 723 TLTFLPVAFIFLISKLPLYIDVVRALVTTVPRRSADVV----PPVDRKPSKPLQKGKFEV 782
           T+  +PVA IF+ISKLPLYIDVV+A+   VP+RSA VV     P+     KP  KGKFEV
Sbjct: 729 TIVLVPVALIFIISKLPLYIDVVQAIFKIVPKRSAHVVLSDPLPLHTPSIKPFPKGKFEV 788

Query: 783 SSIRAEIDSDEI---MPLVIFTPSTQDSYPVILFLPGCVESF-HSDFLRLVASHGFVIVA 842
           +S   E DSD      PL I  P+ Q SYP++ FLPGC   + +S  L+ +AS G VIV 
Sbjct: 789 ASTPVE-DSDAFSPSTPLSILAPTAQGSYPLLFFLPGCAAEYDYSHVLQHIASQGLVIVC 848

Query: 843 P-ELEAKNTES----TSQVK---SSDREMVEEKLSKLSKTTQREGEETPLLSLALASGER 851
           P ++ AK T S    TSQ K   ++DRE+VEE+LS +    + +G ET   SLAL   +R
Sbjct: 849 PLQMRAKATRSEANETSQFKTWDAADRELVEERLSAV--VAELKGGETKSWSLALGY-DR 908

BLAST of Spg038038 vs. NCBI nr
Match: XP_023534306.1 (uncharacterized protein LOC111795904 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 862.4 bits (2227), Expect = 3.2e-246
Identity = 492/954 (51.57%), Postives = 636/954 (66.67%), Query Frame = 0

Query: 3   DDKNNADHQ--LREFLDMNTRRRKWNAVVEKYEEYPELAQKLKLSRQGDTALHLAVIDNQ 62
           ++  N +H+  LR+FL +NT+R +W  V++KYE++PE A +LKL+R GDTALHLAV+DN+
Sbjct: 9   EESMNEEHKATLRDFLYINTKRTEWEKVIKKYEKHPE-ALELKLTRNGDTALHLAVLDNR 68

Query: 63  EEIVEKLVKIICRNPGNEQLLETTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMRNV 122
           E++V+KLV  I ++   ++LLETTN+R  NPLHLAA  GSA MC+AIASA  +LV  RN 
Sbjct: 69  EDVVQKLVNRI-KDSKCDKLLETTNDRKENPLHLAAQMGSATMCNAIASACDKLVHERNK 128

Query: 123 DDETPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELAFQ 182
            DETPL+LAAA+G+ DAF+CLY+FCRN ++RI  NCR  +NGDTVLH ALR++HF+LAFQ
Sbjct: 129 MDETPLYLAAAFGNRDAFFCLYHFCRNVASRITANCRLTSNGDTVLHSALRNEHFDLAFQ 188

Query: 183 LIHMNNDAMSWVNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIETL 242
           ++H+NN+AM WV + G+TPLHVLAS PT+FKSGS IRGW+ I YYCT VD+LKPQPI++L
Sbjct: 189 ILHLNNEAMHWVTKDGVTPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSL 248

Query: 243 SKAWTKRLPKAKRIPYSFPDNYETCIEFFMTLKDAIFK---------------------- 302
            + W  R+         FP NYETCI+FF  + D   K                      
Sbjct: 249 IRDWIDRMSNPNPSTPCFPANYETCIDFFTWVWDGFLKGSGLKRICHDNKNDETKKDTDD 308

Query: 303 ------------------------------------------------------------ 362
                                                                       
Sbjct: 309 AGRNIMVEGGESLEADEPHQRLDTQLLKAHGLAKEASMTNVPRNYNTCIHFFQIVFSAIL 368

Query: 363 -----GSKEIKKIVKKKQKHTWSVQVMEKLFDQFGTPDKYGDDGKIP--DGMATGHEDSE 422
                GS E KKI ++K+KHTWSVQVMEKL  ++  PD+Y  +G IP      T  +  +
Sbjct: 369 ISLGLGSAEFKKIRRQKEKHTWSVQVMEKLL-EYAAPDEYDCNGGIPMDSTSRTPDQAGD 428

Query: 423 TFPYTLEENKVNFSTNVVDKKQNDPEESKDVPDSAMLLAAKNGVLEMVRGLSQRFPLAIH 482
           T PY+ +++ V FS + ++ K  + E+ KD  ++ MLLAAKNGV+E+V+G+ +RFPL+I+
Sbjct: 429 TLPYSFKDDDVLFSVH-IESKPTEAEKPKDFQETPMLLAAKNGVIEIVKGMFRRFPLSIY 488

Query: 483 DTRNDKKNVLLLAAEYRQPQVYRFLHNKKDDLESLFRAVDVDGNGALHLAAAASKSMVWR 542
           D   DKKNV+LLAAEY QP+VYRFL +     E+LFRAVD +GN ALHLAAAASKSM+WR
Sbjct: 489 DAGKDKKNVVLLAAEYGQPEVYRFLLSPGVYKENLFRAVDDNGNSALHLAAAASKSMIWR 548

Query: 543 ITGDALQMQWEVKWYKFIEESMPLHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTS 602
           ITG ALQMQWE+KWYKF+EES+PL+FF HYNKE K A+ IFHETHMD+V+  GDWLT+TS
Sbjct: 549 ITGAALQMQWEIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVQKSGDWLTKTS 608

Query: 603 KSCSVVGTLIVTVAFTSATTIPGGYD-TEGVPFLEKEQSFFIFAVSSLIALCLSSASVTM 662
           KSCSVVG LIVTVAFTS  +IPGG++  +G PFL+  ++FF FA+ SLIALCLSS SVTM
Sbjct: 609 KSCSVVGALIVTVAFTSVASIPGGFNPRDGSPFLQDREAFFTFALFSLIALCLSSTSVTM 668

Query: 663 FLAILTSRFEAKDFGSDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVALLLY 722
           FLAILT RF+A DF ++LPWKLFIG SSLF SI+S LISFCAGHYFL+   +   A LLY
Sbjct: 669 FLAILTHRFDANDFRTNLPWKLFIGFSSLFGSIISMLISFCAGHYFLMHRHIPHHAALLY 728

Query: 723 TLTFLPVAFIFLISKLPLYIDVVRALVTTVPRRSADVV----PPVDRKPSKPLQKGKFEV 782
           T+  +PVA IF+ISKLPLYIDVV+A+   VP+RSA VV     P+     KP  KGKFEV
Sbjct: 729 TIVLVPVALIFIISKLPLYIDVVQAIFKIVPKRSAHVVLSDPLPLHTPSIKPFPKGKFEV 788

Query: 783 SSIRAEIDSDEI---MPLVIFTPSTQDSYPVILFLPGCVESF-HSDFLRLVASHGFVIVA 842
           +S   E DSD      PL I  P+ Q SYP++ FLPGC   + +S  L+ +AS G VIV 
Sbjct: 789 ASTPVE-DSDAFSPSTPLSILAPTAQGSYPLLFFLPGCAAEYDYSHVLQHIASQGLVIVC 848

Query: 843 PELEAKNTES--TSQVK---SSDREMVEEKLSKLSKTTQREGEETPLLSLALASGERPSH 851
           P L+A  +E+  TSQ K   ++DRE+VEE+LS +    + +G ET   SLAL   +RP +
Sbjct: 849 P-LQATRSEANETSQFKTWDAADRELVEERLSAV--VAELKGGETKSWSLALGY-DRPWN 908

BLAST of Spg038038 vs. ExPASy Swiss-Prot
Match: B2RXR6 (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Mus musculus OX=10090 GN=Ankrd44 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.9e-08
Identity = 49/161 (30.43%), Postives = 68/161 (42.24%), Query Frame = 0

Query: 47  QGDTALHLAVIDNQEEIVEKLVKIICRNPGNEQLLETTNERNNNPLHLAAAQGSARMCHA 106
           +G TAL+LA      E VE LV     N G   +    N     PLH +   G       
Sbjct: 599 KGRTALYLAAFKGHTECVEALV-----NQG-ASIFVKDNVTKRTPLHASVINGHTLCLRL 658

Query: 107 I--ASANGRLVDMRNVDDETPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGDT 166
           +   + N  +VD+++   +TPL LA AYGH+DA   L     N  A   + C       T
Sbjct: 659 LLETADNPEVVDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDAVDIVGC-------T 718

Query: 167 VLHRALRDDHFELAFQLIHMNNDAMSWVNEKGLTPLHVLAS 206
            LHR +   H E    L+      +   + +G TPLH  A+
Sbjct: 719 ALHRGIMTGHEECVQMLLEQEASILC-KDSRGRTPLHYAAA 745

BLAST of Spg038038 vs. ExPASy Swiss-Prot
Match: Q01484 (Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=4)

HSP 1 Score: 60.8 bits (146), Expect = 8.4e-08
Identity = 48/163 (29.45%), Postives = 71/163 (43.56%), Query Frame = 0

Query: 44  LSRQGDTALHLAVIDNQEEIVEKLVKIICRNPGNEQLLETTNERNNNPLHLAAAQGSARM 103
           ++  G T +H+A       IV  L++       N    + TN R    LH+AA  G   +
Sbjct: 427 ITESGLTPIHVAAFMGHLNIVLLLLQ-------NGASPDVTNIRGETALHMAARAGQVEV 486

Query: 104 CHAIASANGRLVDMRNVDDETPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGD 163
              +   NG LVD R  +++TPL +A+  G  +    L     +  A          NG 
Sbjct: 487 VRCLL-RNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDA-------ATTNGY 546

Query: 164 TVLHRALRDDHFELAFQLIHMNNDAMSWVNEKGLTPLHVLASY 207
           T LH + R+   ++A  L+     A S   +KG TPLHV A Y
Sbjct: 547 TPLHISAREGQVDVASVLLEA-GAAHSLATKKGFTPLHVAAKY 573

BLAST of Spg038038 vs. ExPASy Swiss-Prot
Match: Q8C8R3 (Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 8.4e-08
Identity = 48/163 (29.45%), Postives = 71/163 (43.56%), Query Frame = 0

Query: 44  LSRQGDTALHLAVIDNQEEIVEKLVKIICRNPGNEQLLETTNERNNNPLHLAAAQGSARM 103
           ++  G T +H+A       IV  L++       N    + TN R    LH+AA  G   +
Sbjct: 427 ITESGLTPIHVAAFMGHLNIVLLLLQ-------NGASPDVTNIRGETALHMAARAGQVEV 486

Query: 104 CHAIASANGRLVDMRNVDDETPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGD 163
              +   NG LVD R  +++TPL +A+  G  +    L     +  A          NG 
Sbjct: 487 VRCLL-RNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDA-------ATTNGY 546

Query: 164 TVLHRALRDDHFELAFQLIHMNNDAMSWVNEKGLTPLHVLASY 207
           T LH + R+   ++A  L+     A S   +KG TPLHV A Y
Sbjct: 547 TPLHISAREGQVDVASVLLEA-GAAHSLATKKGFTPLHVAAKY 573

BLAST of Spg038038 vs. ExPASy Swiss-Prot
Match: Q8N8A2 (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Homo sapiens OX=9606 GN=ANKRD44 PE=1 SV=3)

HSP 1 Score: 58.5 bits (140), Expect = 4.2e-07
Identity = 50/162 (30.86%), Postives = 67/162 (41.36%), Query Frame = 0

Query: 47  QGDTALHLAVIDNQEEIVEKLVKIICRNPGNEQLLETTNERNNNPLHLAAAQGSA---RM 106
           +G TAL LA      E VE L+     N G   +    N     PLH +   G     R+
Sbjct: 599 KGRTALDLAAFKGHTECVEALI-----NQG-ASIFVKDNVTKRTPLHASVINGHTLCLRL 658

Query: 107 CHAIASANGRLVDMRNVDDETPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGD 166
              IA  N   VD+++   +TPL LA AYGH+DA   L     N      + C       
Sbjct: 659 LLEIAD-NPEAVDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDTVDILGC------- 718

Query: 167 TVLHRALRDDHFELAFQLIHMNNDAMSWVNEKGLTPLHVLAS 206
           T LHR +   H E    L+      +   + +G TPLH  A+
Sbjct: 719 TALHRGIMTGHEECVQMLLEQEVSILC-KDSRGRTPLHYAAA 745

BLAST of Spg038038 vs. ExPASy Swiss-Prot
Match: P16157 (Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=3)

HSP 1 Score: 57.4 bits (137), Expect = 9.3e-07
Identity = 47/163 (28.83%), Postives = 70/163 (42.94%), Query Frame = 0

Query: 44  LSRQGDTALHLAVIDNQEEIVEKLVKIICRNPGNEQLLETTNERNNNPLHLAAAQGSARM 103
           ++  G T LH+A       IV+ L++              +N +   PLH+AA  G   +
Sbjct: 400 VTESGLTPLHVASFMGHLPIVKNLLQ-------RGASPNVSNVKVETPLHMAARAGHTEV 459

Query: 104 CHAIASANGRLVDMRNVDDETPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGD 163
              +   N   V+ +  DD+TPL  AA  GH +    L     N +  +A        G 
Sbjct: 460 AKYLLQ-NKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLE--NNANPNLATTA-----GH 519

Query: 164 TVLHRALRDDHFELAFQLIHMNNDAMSWVNEKGLTPLHVLASY 207
           T LH A R+ H E    L+     + + + +KG TPLHV A Y
Sbjct: 520 TPLHIAAREGHVETVLALLE-KEASQACMTKKGFTPLHVAAKY 546

BLAST of Spg038038 vs. ExPASy TrEMBL
Match: A0A6J1K2F1 (uncharacterized protein LOC111491104 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491104 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 5.2e-255
Identity = 510/945 (53.97%), Postives = 632/945 (66.88%), Query Frame = 0

Query: 12  LREFLDMNTRRRKWNAVVEKYEEYPELAQKLKLSRQGDTALHLAVIDNQEEIVEKLVKII 71
           LR+FL  NT+R KW  V++KYEEYPE AQ+LKL+R GDTALHLAV+DN+EE+V+KLV  I
Sbjct: 34  LRDFLYTNTKRGKWEEVIKKYEEYPE-AQELKLTRNGDTALHLAVLDNREEVVQKLVNRI 93

Query: 72  CRNPGNEQLLETTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMRNVDDETPLFLAAA 131
                 ++LL+TTN+R   PLHLAA  GSA MC+AIASA+  LVD+RN  DETPL+LAAA
Sbjct: 94  KHTSKYDELLKTTNDREETPLHLAAQMGSATMCNAIASAHDELVDLRNKVDETPLYLAAA 153

Query: 132 YGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELAFQLIHMNNDAMSW 191
            G+ DAF+CLY+FCRN+++RI  NCR  +NGDTVLH ALR+DHF+LAFQ++H+NN+AM W
Sbjct: 154 SGNRDAFFCLYHFCRNNASRITANCRLTSNGDTVLHSALRNDHFDLAFQILHLNNEAMHW 213

Query: 192 VNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIETLSKAWTKRLPKA 251
           V E G+TPLHVLAS PT+FKSGS IRGW+ I YYCT VD+LKPQPI++L + W  R+   
Sbjct: 214 VTETGITPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSLIRDWIDRMSNP 273

Query: 252 KRIPYSFPDNYETCIEFFMTLKDAIFK--------------------------------- 311
                 FP NY TCI+FF  + D   K                                 
Sbjct: 274 NTSTPCFPANYGTCIDFFTWVWDGFLKGSGLKRICHDNENDESKKDTQVVRNIMVEGGES 333

Query: 312 -----------------------------------------------------GSKEIKK 371
                                                                GS E KK
Sbjct: 334 LETDESHQRLDTELLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAIMISLGLGSAEFKK 393

Query: 372 IVKKKQKHTWSVQVMEKLFDQFGTPDKYGDDGKIP-DGMATGHEDSE-TFPYTLEENKVN 431
           I +KK+KHTWSVQVMEKL  ++  PD+Y  +G  P D  +   + +E T PY+L   +V 
Sbjct: 394 IRRKKEKHTWSVQVMEKLL-EYAPPDEYECNGGTPMDSTSQTPDQAEVTLPYSLVAGEVR 453

Query: 432 FSTNVVDKKQNDPEESKDVPDSAMLLAAKNGVLEMVRGLSQRFPLAIHDTRNDKKNVLLL 491
            S N ++ K  + E+ K+V ++AMLLAAKNGV+E+V+G+  RFPL+I D R DKKNV+LL
Sbjct: 454 LS-NSIESKPKEAEKPKNVQETAMLLAAKNGVIEIVKGMFHRFPLSICDARKDKKNVVLL 513

Query: 492 AAEYRQPQVYRFLHNKKDDLESLFRAVDVDGNGALHLAAAASKSMVWRITGDALQMQWEV 551
           AAEY QP VYRFL + K   E+LFRAVD +GN ALHLAAAASKSM+WRITG ALQMQWE+
Sbjct: 514 AAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIWRITGAALQMQWEI 573

Query: 552 KWYKFIEESMPLHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTSKSCSVVGTLIVT 611
           KWYKF+EES+PL+FF HYNKE K A+ IFHETHMD+VK  G+WLT+TSKSCSVVGTLIVT
Sbjct: 574 KWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVKKSGEWLTKTSKSCSVVGTLIVT 633

Query: 612 VAFTSATTIPGGYDT-EGVPFLEKEQSFFIFAVSSLIALCLSSASVTMFLAILTSRFEAK 671
           VAFTS  +IPGG++  +G PFL+  ++FF FA+ SLIALCLSS SVTMFLAILT RF+A 
Sbjct: 634 VAFTSVASIPGGFNPHDGSPFLKDRKAFFTFALFSLIALCLSSTSVTMFLAILTYRFDAN 693

Query: 672 DFGSDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVALLLYTLTFLPVAFIFL 731
           DF ++LPWKLFIG SSLF SI+S L+SFCAGHYFL+   +   A LLYT+  +PVA IF+
Sbjct: 694 DFRTNLPWKLFIGFSSLFGSIISMLVSFCAGHYFLMHQHIPHHAALLYTIVLVPVALIFI 753

Query: 732 ISKLPLYIDVVRALVTTVPRRSADVV---PPVDRKPS-KPLQKGKFEVSSIRAEIDSDEI 791
           ISKLPLYIDVV+A+   VP RSA VV   P     PS KP QKGKFEV+SI  E DSD  
Sbjct: 754 ISKLPLYIDVVQAIFKIVPNRSAHVVLSDPLPPHTPSIKPFQKGKFEVTSIPVE-DSDAF 813

Query: 792 M---PLVIFTPSTQDSYPVILFLPGCVESFHSDFLRLVASHGFVIVAP-ELEAKNTES-- 851
               PL+I TP+ Q SYP++ FLPGC E  +S FL+L+AS G VIV P ++ A+ T S  
Sbjct: 814 SPSNPLLILTPTAQGSYPLLFFLPGCAEYDYSHFLQLIASQGLVIVWPLQMSAEATRSEM 873

BLAST of Spg038038 vs. ExPASy TrEMBL
Match: A0A6J1JZF8 (uncharacterized protein LOC111491104 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111491104 PE=3 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 1.5e-254
Identity = 511/944 (54.13%), Postives = 632/944 (66.95%), Query Frame = 0

Query: 12  LREFLDMNTRRRKWNAVVEKYEEYPELAQKLKLSRQGDTALHLAVIDNQEEIVEKLVKII 71
           LR+FL  NT+R KW  V++KYEEYPE AQ+LKL+R GDTALHLAV+DN+EE+V+KLV  I
Sbjct: 34  LRDFLYTNTKRGKWEEVIKKYEEYPE-AQELKLTRNGDTALHLAVLDNREEVVQKLVNRI 93

Query: 72  CRNPGNEQLLETTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMRNVDDETPLFLAAA 131
                 ++LL+TTN+R   PLHLAA  GSA MC+AIASA+  LVD+RN  DETPL+LAAA
Sbjct: 94  KHTSKYDELLKTTNDREETPLHLAAQMGSATMCNAIASAHDELVDLRNKVDETPLYLAAA 153

Query: 132 YGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELAFQLIHMNNDAMSW 191
            G+ DAF+CLY+FCRN+++RI  NCR  +NGDTVLH ALR+DHF+LAFQ++H+NN+AM W
Sbjct: 154 SGNRDAFFCLYHFCRNNASRITANCRLTSNGDTVLHSALRNDHFDLAFQILHLNNEAMHW 213

Query: 192 VNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIETLSKAWTKRLPKA 251
           V E G+TPLHVLAS PT+FKSGS IRGW+ I YYCT VD+LKPQPI++L + W  R+   
Sbjct: 214 VTETGITPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSLIRDWIDRMSNP 273

Query: 252 KRIPYSFPDNYETCIEFFMTLKDAIFK--------------------------------- 311
                 FP NY TCI+FF  + D   K                                 
Sbjct: 274 NTSTPCFPANYGTCIDFFTWVWDGFLKGSGLKRICHDNENDESKKDTQVVRNIMVEGGES 333

Query: 312 -----------------------------------------------------GSKEIKK 371
                                                                GS E KK
Sbjct: 334 LETDESHQRLDTELLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAIMISLGLGSAEFKK 393

Query: 372 IVKKKQKHTWSVQVMEKLFDQFGTPDKYGDDGKIP-DGMATGHEDSE-TFPYTLEENKVN 431
           I +KK+KHTWSVQVMEKL  ++  PD+Y  +G  P D  +   + +E T PY+L   +V 
Sbjct: 394 IRRKKEKHTWSVQVMEKLL-EYAPPDEYECNGGTPMDSTSQTPDQAEVTLPYSLVAGEVR 453

Query: 432 FSTNVVDKKQNDPEESKDV--PDSAMLLAAKNGVLEMVRGLSQRFPLAIHDTRNDKKNVL 491
            S N ++ K  + E+ K+V  P++AMLLAAKNGV+E+V+G+  RFPL+I D R DKKNV+
Sbjct: 454 LS-NSIESKPKEAEKPKNVQAPETAMLLAAKNGVIEIVKGMFHRFPLSICDARKDKKNVV 513

Query: 492 LLAAEYRQPQVYRFLHNKKDDLESLFRAVDVDGNGALHLAAAASKSMVWRITGDALQMQW 551
           LLAAEY QP VYRFL + K   E+LFRAVD +GN ALHLAAAASKSM+WRITG ALQMQW
Sbjct: 514 LLAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIWRITGAALQMQW 573

Query: 552 EVKWYKFIEESMPLHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTSKSCSVVGTLI 611
           E+KWYKF+EES+PL+FF HYNKE K A+ IFHETHMD+VK  G+WLT+TSKSCSVVGTLI
Sbjct: 574 EIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVKKSGEWLTKTSKSCSVVGTLI 633

Query: 612 VTVAFTSATTIPGGYDT-EGVPFLEKEQSFFIFAVSSLIALCLSSASVTMFLAILTSRFE 671
           VTVAFTS  +IPGG++  +G PFL+  ++FF FA+ SLIALCLSS SVTMFLAILT RF+
Sbjct: 634 VTVAFTSVASIPGGFNPHDGSPFLKDRKAFFTFALFSLIALCLSSTSVTMFLAILTYRFD 693

Query: 672 AKDFGSDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVALLLYTLTFLPVAFI 731
           A DF ++LPWKLFIG SSLF SI+S L+SFCAGHYFL+   +   A LLYT+  +PVA I
Sbjct: 694 ANDFRTNLPWKLFIGFSSLFGSIISMLVSFCAGHYFLMHQHIPHHAALLYTIVLVPVALI 753

Query: 732 FLISKLPLYIDVVRALVTTVPRRSADVV---PPVDRKPS-KPLQKGKFEVSSIRAEIDSD 791
           F+ISKLPLYIDVV+A+   VP RSA VV   P     PS KP QKGKFEV+SI  E DSD
Sbjct: 754 FIISKLPLYIDVVQAIFKIVPNRSAHVVLSDPLPPHTPSIKPFQKGKFEVTSIPVE-DSD 813

Query: 792 EIM---PLVIFTPSTQDSYPVILFLPGCVESFHSDFLRLVASHGFVIVAPELEAKNTE-- 851
                 PL+I TP+ Q SYP++ FLPGC E  +S FL+L+AS G VIV P L+A  +E  
Sbjct: 814 AFSPSNPLLILTPTAQGSYPLLFFLPGCAEYDYSHFLQLIASQGLVIVWP-LQATRSEMN 873

BLAST of Spg038038 vs. ExPASy TrEMBL
Match: A0A6J1K4G3 (uncharacterized protein LOC111491104 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491104 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 2.0e-254
Identity = 511/947 (53.96%), Postives = 633/947 (66.84%), Query Frame = 0

Query: 12  LREFLDMNTRRRKWNAVVEKYEEYPELAQKLKLSRQGDTALHLAVIDNQEEIVEKLVKII 71
           LR+FL  NT+R KW  V++KYEEYPE AQ+LKL+R GDTALHLAV+DN+EE+V+KLV  I
Sbjct: 34  LRDFLYTNTKRGKWEEVIKKYEEYPE-AQELKLTRNGDTALHLAVLDNREEVVQKLVNRI 93

Query: 72  CRNPGNEQLLETTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMRNVDDETPLFLAAA 131
                 ++LL+TTN+R   PLHLAA  GSA MC+AIASA+  LVD+RN  DETPL+LAAA
Sbjct: 94  KHTSKYDELLKTTNDREETPLHLAAQMGSATMCNAIASAHDELVDLRNKVDETPLYLAAA 153

Query: 132 YGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELAFQLIHMNNDAMSW 191
            G+ DAF+CLY+FCRN+++RI  NCR  +NGDTVLH ALR+DHF+LAFQ++H+NN+AM W
Sbjct: 154 SGNRDAFFCLYHFCRNNASRITANCRLTSNGDTVLHSALRNDHFDLAFQILHLNNEAMHW 213

Query: 192 VNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIETLSKAWTKRLPKA 251
           V E G+TPLHVLAS PT+FKSGS IRGW+ I YYCT VD+LKPQPI++L + W  R+   
Sbjct: 214 VTETGITPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLKPQPIDSLIRDWIDRMSNP 273

Query: 252 KRIPYSFPDNYETCIEFFMTLKDAIFK--------------------------------- 311
                 FP NY TCI+FF  + D   K                                 
Sbjct: 274 NTSTPCFPANYGTCIDFFTWVWDGFLKGSGLKRICHDNENDESKKDTQVVRNIMVEGGES 333

Query: 312 -----------------------------------------------------GSKEIKK 371
                                                                GS E KK
Sbjct: 334 LETDESHQRLDTELLKAHGLAKEASITNVPRNYNTCIHFFQIVFSAIMISLGLGSAEFKK 393

Query: 372 IVKKKQKHTWSVQVMEKLFDQFGTPDKYGDDGKIP-DGMATGHEDSE-TFPYTLEENKVN 431
           I +KK+KHTWSVQVMEKL  ++  PD+Y  +G  P D  +   + +E T PY+L   +V 
Sbjct: 394 IRRKKEKHTWSVQVMEKLL-EYAPPDEYECNGGTPMDSTSQTPDQAEVTLPYSLVAGEVR 453

Query: 432 FSTNVVDKKQNDPEESKDV--PDSAMLLAAKNGVLEMVRGLSQRFPLAIHDTRNDKKNVL 491
            S N ++ K  + E+ K+V  P++AMLLAAKNGV+E+V+G+  RFPL+I D R DKKNV+
Sbjct: 454 LS-NSIESKPKEAEKPKNVQAPETAMLLAAKNGVIEIVKGMFHRFPLSICDARKDKKNVV 513

Query: 492 LLAAEYRQPQVYRFLHNKKDDLESLFRAVDVDGNGALHLAAAASKSMVWRITGDALQMQW 551
           LLAAEY QP VYRFL + K   E+LFRAVD +GN ALHLAAAASKSM+WRITG ALQMQW
Sbjct: 514 LLAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSMIWRITGAALQMQW 573

Query: 552 EVKWYKFIEESMPLHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTSKSCSVVGTLI 611
           E+KWYKF+EES+PL+FF HYNKE K A+ IFHETHMD+VK  G+WLT+TSKSCSVVGTLI
Sbjct: 574 EIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVKKSGEWLTKTSKSCSVVGTLI 633

Query: 612 VTVAFTSATTIPGGYDT-EGVPFLEKEQSFFIFAVSSLIALCLSSASVTMFLAILTSRFE 671
           VTVAFTS  +IPGG++  +G PFL+  ++FF FA+ SLIALCLSS SVTMFLAILT RF+
Sbjct: 634 VTVAFTSVASIPGGFNPHDGSPFLKDRKAFFTFALFSLIALCLSSTSVTMFLAILTYRFD 693

Query: 672 AKDFGSDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVALLLYTLTFLPVAFI 731
           A DF ++LPWKLFIG SSLF SI+S L+SFCAGHYFL+   +   A LLYT+  +PVA I
Sbjct: 694 ANDFRTNLPWKLFIGFSSLFGSIISMLVSFCAGHYFLMHQHIPHHAALLYTIVLVPVALI 753

Query: 732 FLISKLPLYIDVVRALVTTVPRRSADVV---PPVDRKPS-KPLQKGKFEVSSIRAEIDSD 791
           F+ISKLPLYIDVV+A+   VP RSA VV   P     PS KP QKGKFEV+SI  E DSD
Sbjct: 754 FIISKLPLYIDVVQAIFKIVPNRSAHVVLSDPLPPHTPSIKPFQKGKFEVTSIPVE-DSD 813

Query: 792 EIM---PLVIFTPSTQDSYPVILFLPGCVESFHSDFLRLVASHGFVIVAP-ELEAKNTES 851
                 PL+I TP+ Q SYP++ FLPGC E  +S FL+L+AS G VIV P ++ A+ T S
Sbjct: 814 AFSPSNPLLILTPTAQGSYPLLFFLPGCAEYDYSHFLQLIASQGLVIVWPLQMSAEATRS 873

BLAST of Spg038038 vs. ExPASy TrEMBL
Match: A0A6J1EX58 (uncharacterized protein LOC111437338 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437338 PE=3 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 3.8e-245
Identity = 493/962 (51.25%), Postives = 633/962 (65.80%), Query Frame = 0

Query: 1   MSDDKNNADHQ--LREFLDMNTRRRKWNAVVEKYEEYPELAQKLKLSRQGDTALHLAVID 60
           + ++  N  H+  LR+FL +NT+R +W  V++KYE++PE AQ LKL+R GDTALHLAV+D
Sbjct: 21  IKEESMNEKHEATLRDFLYINTKRTEWEKVIKKYEKHPE-AQGLKLTRNGDTALHLAVLD 80

Query: 61  NQEEIVEKLVKIICRNPGNEQLLETTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMR 120
           N+EE+V+KLV  I ++   ++LLETTN+R  NPLHLAA  GSA MC+AIASA+ +LV+ R
Sbjct: 81  NREEMVQKLVNRI-KDSKRDELLETTNDRKENPLHLAAQMGSATMCYAIASAHHKLVEKR 140

Query: 121 NVDDETPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELA 180
           N  DETPL+LAAA G+ DAF+CLY+FCR+  + I  NCR  +NGDTVLH ALR+DHF+LA
Sbjct: 141 NKIDETPLYLAAASGNRDAFFCLYHFCRDLDSGITANCRLSSNGDTVLHSALRNDHFDLA 200

Query: 181 FQLIHMNNDAMSWVNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIE 240
           F ++H++N+AM WV + G+TPLHVLAS PT+FKSGS IRGW+ I YYCT VD+L PQPI+
Sbjct: 201 FHILHLHNEAMHWVTKDGVTPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLNPQPID 260

Query: 241 TLSKAWTKRLPKAKRIPYSFPDNYETCIEFFMTLKDAIFK-------------------- 300
           +L + W  R+         FP NYETCI+FF  + D   K                    
Sbjct: 261 SLIRDWIDRMSNPNTSTPCFPANYETCIDFFTWVWDGFLKGSGLKRICHDFKNDESKKDT 320

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 321 DDAGRNIMVEGGESSEAAEPHQRLDTQLLKAHGLAKEASITNVPRNYNTCIHFFQIVFSA 380

Query: 361 -------GSKEIKKIVKKKQKHTWSVQVMEKLFDQFGTPDKYGDDGKIP--DGMATGHED 420
                  GS E KKI ++K+KHTWSVQVMEKL  ++  PD+Y  +G IP      T  + 
Sbjct: 381 ILISLGWGSAEFKKIRRQKEKHTWSVQVMEKLL-EYAAPDEYDCNGGIPMDSTSQTPDQA 440

Query: 421 SETFPYTLEENKVNFSTNVVDK-KQNDPEESKDVPDSAMLLAAKNGVLEMVRGLSQRFPL 480
             T PY+ +++ V FS ++  K  + +  + KD  ++ MLLAAKNGV+E+V+G+  RFPL
Sbjct: 441 GVTLPYSFQDDDVLFSVHIESKPTEAEKPKPKDFQETPMLLAAKNGVIEIVKGMFCRFPL 500

Query: 481 AIHDTRNDKKNVLLLAAEYRQPQVYRFLHNKKDDLESLFRAVDVDGNGALHLAAAASKSM 540
           +I+D   DKKNV+LLAAEY QP VYRFL + K   E+LFRAVD +GN ALHLAAAASKSM
Sbjct: 501 SIYDAGKDKKNVVLLAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAASKSM 560

Query: 541 VWRITGDALQMQWEVKWYKFIEESMPLHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLT 600
           +WRITG ALQMQWE+KWYKF+EES+PL+FF HYNKE K A+ IFHETHMD+V+  GDWL 
Sbjct: 561 IWRITGAALQMQWEIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVQKSGDWLI 620

Query: 601 QTSKSCSVVGTLIVTVAFTSATTIPGGYD-TEGVPFLEKEQSFFIFAVSSLIALCLSSAS 660
           +TSKSCSVVG LIVTVAFTS  +IPGG++  +G PFL+  ++FF FA+ SLIALCLSS S
Sbjct: 621 KTSKSCSVVGALIVTVAFTSVASIPGGFNPRDGSPFLQDREAFFTFALFSLIALCLSSTS 680

Query: 661 VTMFLAILTSRFEAKDFGSDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVAL 720
           VT+FLAILT RF+A DF ++LPWKLFIG SSLF SI+S LISFCAGHYFL+   +   A 
Sbjct: 681 VTIFLAILTHRFDANDFRTNLPWKLFIGFSSLFGSIISMLISFCAGHYFLMHRHIPHHAA 740

Query: 721 LLYTLTFLPVAFIFLISKLPLYIDVVRALVTTVPRRSADVV----PPVDRKPSKPLQKGK 780
           LLYT+  +PVA IF+ISKLPLYIDVV+A+   VP+RSA VV     P+     KP +KGK
Sbjct: 741 LLYTIVLVPVALIFIISKLPLYIDVVQAIFKIVPKRSAHVVLSDPLPLHTPSVKPFRKGK 800

Query: 781 FEVSSIRAEIDSDEI---MPLVIFTPSTQDSYPVILFLPGCVESF-HSDFLRLVASHGFV 840
           FEV+S   E DSD      PL I  P+ Q SYP++ FLPGC   + +S FL+ +AS G V
Sbjct: 801 FEVTSTAME-DSDAFSPSTPLSILAPTAQGSYPLLFFLPGCAAEYDYSHFLQRIASQGLV 860

Query: 841 IVAP-ELEAKNTES----TSQVK---SSDREMVEEKLSKLSKTTQREGEETPLLSLALAS 853
           IV P ++ AK T S    TSQ K   ++DREMVEE+LS +    + +G +T   SLAL  
Sbjct: 861 IVCPLQMRAKATRSEANETSQFKTWDAADREMVEERLSGV--VAELKGGKTKSWSLALGY 920

BLAST of Spg038038 vs. ExPASy TrEMBL
Match: A0A6J1ESA5 (uncharacterized protein LOC111437338 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437338 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 6.4e-245
Identity = 494/965 (51.19%), Postives = 634/965 (65.70%), Query Frame = 0

Query: 1   MSDDKNNADHQ--LREFLDMNTRRRKWNAVVEKYEEYPELAQKLKLSRQGDTALHLAVID 60
           + ++  N  H+  LR+FL +NT+R +W  V++KYE++PE AQ LKL+R GDTALHLAV+D
Sbjct: 21  IKEESMNEKHEATLRDFLYINTKRTEWEKVIKKYEKHPE-AQGLKLTRNGDTALHLAVLD 80

Query: 61  NQEEIVEKLVKIICRNPGNEQLLETTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMR 120
           N+EE+V+KLV  I ++   ++LLETTN+R  NPLHLAA  GSA MC+AIASA+ +LV+ R
Sbjct: 81  NREEMVQKLVNRI-KDSKRDELLETTNDRKENPLHLAAQMGSATMCYAIASAHHKLVEKR 140

Query: 121 NVDDETPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELA 180
           N  DETPL+LAAA G+ DAF+CLY+FCR+  + I  NCR  +NGDTVLH ALR+DHF+LA
Sbjct: 141 NKIDETPLYLAAASGNRDAFFCLYHFCRDLDSGITANCRLSSNGDTVLHSALRNDHFDLA 200

Query: 181 FQLIHMNNDAMSWVNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIE 240
           F ++H++N+AM WV + G+TPLHVLAS PT+FKSGS IRGW+ I YYCT VD+L PQPI+
Sbjct: 201 FHILHLHNEAMHWVTKDGVTPLHVLASKPTAFKSGSQIRGWRNIAYYCTHVDQLNPQPID 260

Query: 241 TLSKAWTKRLPKAKRIPYSFPDNYETCIEFFMTLKDAIFK-------------------- 300
           +L + W  R+         FP NYETCI+FF  + D   K                    
Sbjct: 261 SLIRDWIDRMSNPNTSTPCFPANYETCIDFFTWVWDGFLKGSGLKRICHDFKNDESKKDT 320

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 321 DDAGRNIMVEGGESSEAAEPHQRLDTQLLKAHGLAKEASITNVPRNYNTCIHFFQIVFSA 380

Query: 361 -------GSKEIKKIVKKKQKHTWSVQVMEKLFDQFGTPDKYGDDGKIP--DGMATGHED 420
                  GS E KKI ++K+KHTWSVQVMEKL  ++  PD+Y  +G IP      T  + 
Sbjct: 381 ILISLGWGSAEFKKIRRQKEKHTWSVQVMEKLL-EYAAPDEYDCNGGIPMDSTSQTPDQA 440

Query: 421 SETFPYTLEENKVNFSTNVVDKKQNDPEESK----DVPDSAMLLAAKNGVLEMVRGLSQR 480
             T PY+ +++ V FS + ++ K  + E+ K      P++ MLLAAKNGV+E+V+G+  R
Sbjct: 441 GVTLPYSFQDDDVLFSVH-IESKPTEAEKPKPKDFQAPETPMLLAAKNGVIEIVKGMFCR 500

Query: 481 FPLAIHDTRNDKKNVLLLAAEYRQPQVYRFLHNKKDDLESLFRAVDVDGNGALHLAAAAS 540
           FPL+I+D   DKKNV+LLAAEY QP VYRFL + K   E+LFRAVD +GN ALHLAAAAS
Sbjct: 501 FPLSIYDAGKDKKNVVLLAAEYGQPDVYRFLLSPKVYKENLFRAVDDNGNSALHLAAAAS 560

Query: 541 KSMVWRITGDALQMQWEVKWYKFIEESMPLHFFTHYNKEEKTASTIFHETHMDMVKNGGD 600
           KSM+WRITG ALQMQWE+KWYKF+EES+PL+FF HYNKE K A+ IFHETHMD+V+  GD
Sbjct: 561 KSMIWRITGAALQMQWEIKWYKFVEESVPLYFFAHYNKEGKNATAIFHETHMDLVQKSGD 620

Query: 601 WLTQTSKSCSVVGTLIVTVAFTSATTIPGGYD-TEGVPFLEKEQSFFIFAVSSLIALCLS 660
           WL +TSKSCSVVG LIVTVAFTS  +IPGG++  +G PFL+  ++FF FA+ SLIALCLS
Sbjct: 621 WLIKTSKSCSVVGALIVTVAFTSVASIPGGFNPRDGSPFLQDREAFFTFALFSLIALCLS 680

Query: 661 SASVTMFLAILTSRFEAKDFGSDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLRS 720
           S SVT+FLAILT RF+A DF ++LPWKLFIG SSLF SI+S LISFCAGHYFL+   +  
Sbjct: 681 STSVTIFLAILTHRFDANDFRTNLPWKLFIGFSSLFGSIISMLISFCAGHYFLMHRHIPH 740

Query: 721 VALLLYTLTFLPVAFIFLISKLPLYIDVVRALVTTVPRRSADVV----PPVDRKPSKPLQ 780
            A LLYT+  +PVA IF+ISKLPLYIDVV+A+   VP+RSA VV     P+     KP +
Sbjct: 741 HAALLYTIVLVPVALIFIISKLPLYIDVVQAIFKIVPKRSAHVVLSDPLPLHTPSVKPFR 800

Query: 781 KGKFEVSSIRAEIDSDEI---MPLVIFTPSTQDSYPVILFLPGCVESF-HSDFLRLVASH 840
           KGKFEV+S   E DSD      PL I  P+ Q SYP++ FLPGC   + +S FL+ +AS 
Sbjct: 801 KGKFEVTSTAME-DSDAFSPSTPLSILAPTAQGSYPLLFFLPGCAAEYDYSHFLQRIASQ 860

Query: 841 GFVIVAP-ELEAKNTES----TSQVK---SSDREMVEEKLSKLSKTTQREGEETPLLSLA 853
           G VIV P ++ AK T S    TSQ K   ++DREMVEE+LS +    + +G +T   SLA
Sbjct: 861 GLVIVCPLQMRAKATRSEANETSQFKTWDAADREMVEERLSGV--VAELKGGKTKSWSLA 920

BLAST of Spg038038 vs. TAIR 10
Match: AT3G18670.1 (Ankyrin repeat family protein )

HSP 1 Score: 165.6 bits (418), Expect = 1.7e-40
Identity = 148/614 (24.10%), Postives = 264/614 (43.00%), Query Frame = 0

Query: 44  LSRQGDTALHLAVIDNQEEIVEKLVKIICRNPGNEQLLETTNERNNNPLHLAAAQGSARM 103
           L+  GDT +H AV+    +IVE++++ I  +P  EQ+L+  N+     L  AA  G  R+
Sbjct: 80  LTSNGDTPIHKAVLSGHIKIVEEIIRRI-HDP--EQVLKIKNDNGYTALTYAATGGIVRI 139

Query: 104 CHAIASANGRLVDMRNVDDETPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGD 163
              + +    LV +RN  +  P+ +A+ YGH      LY                     
Sbjct: 140 AECLVNKCPGLVSVRNAKEHIPIVVASLYGHKHLVQYLY--------------------- 199

Query: 164 TVLHRALRDDHFELAFQLIHMNNDAMSWVNEKGLTPLHVLASYPTSFKSGSHIRGWQEIV 223
              H  L D         +   +D+     + G               +   + G     
Sbjct: 200 --SHTPLSD---------LDPCDDSDEHKGKNG-----------AMLVTNCIVDG----- 259

Query: 224 YYCTCVDKLKPQPIETLSKAWTKRLPKAKRIPYSFPDNYETCIEFFMTLKDAIFKGSKEI 283
            YC  +D ++  P                ++ Y+   + +T I        A     + I
Sbjct: 260 LYCIALDLIQRYP----------------KLAYTRDSDNDTAIMALAQTPYAFPSVPRII 319

Query: 284 KKIVKKKQKHTWSVQVMEKLFDQFGTPDKYGDDGKIPDGMATGHEDSETFPYTLEENKVN 343
           +++ K K  H  + ++++ +  +            IP                       
Sbjct: 320 RRVYKLKLGHAQAKEILDCICQE------------IP----------------------- 379

Query: 344 FSTNVVDKKQNDPEESKDVPDSAMLLAAKNGVLEMVRGLSQRFPLAIHDTRNDKKNVLLL 403
                   K +  ++     + A+  A +NG++E +  + + +P  +    +   N+   
Sbjct: 380 --------KFDAAQQKNAGLNQALFKAVENGIVEYIEEMMRHYPDIVWSKNSSGLNIFFY 439

Query: 404 AAEYRQPQVYRFLHNKKDDLESLFRAVDVDGNGALHLAA-AASKSMVWRITGDALQMQWE 463
           A   RQ +++  ++N       L    D+  N  LH AA  A  S +  I G ALQMQ E
Sbjct: 440 AVSQRQEKIFSLIYNIGAKKNILATNWDIFHNNMLHHAAYRAPASRLNLIPGAALQMQRE 499

Query: 464 VKWYKFIEESM-PLHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTSKSCSVVGTLI 523
           ++W+K +E+ + P H      K++KT   +F + H D+V+ G  W+ +T+ SC+VV  LI
Sbjct: 500 LQWFKEVEKLVQPKHRKMVNLKQKKTPKALFTDQHKDLVEQGEKWMKETATSCTVVAALI 559

Query: 524 VTVAFTSATTIPGGYDTEGVPFLEKEQSFFIFAVSSLIALCLSSASVTMFLAILTSRFEA 583
            T+ F+SA T+PGGY ++G+P    +  F IF +S  I+L  S  S+ MFL IL SR+  
Sbjct: 560 TTMMFSSAFTVPGGYRSDGMPLYIHQHRFKIFLISDAISLFTSCMSLLMFLGILKSRYRE 582

Query: 584 KDFGSDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVALLLYTLTFLPVAFIF 643
           +DF   LP KL +GL +LF S+ + +++F      L+  ++  V+     L  +P+  +F
Sbjct: 620 EDFLRSLPTKLIVGLLALFLSMATMIVTFVVTLMTLVGEKISWVSAQFMFLAVIPLG-MF 582

Query: 644 LISKLPLYIDVVRA 656
           ++ + P+ +++ RA
Sbjct: 680 VVLQFPVLLEIFRA 582

BLAST of Spg038038 vs. TAIR 10
Match: AT5G04700.1 (Ankyrin repeat family protein )

HSP 1 Score: 157.1 bits (396), Expect = 6.1e-38
Identity = 155/600 (25.83%), Postives = 266/600 (44.33%), Query Frame = 0

Query: 66  KLVKIICRNPGNEQLLE--TTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMRNVDDE 125
           ++VK + R    EQ+L   + N   + PL + A  G+  +  A+ + N +L+++  ++ +
Sbjct: 128 EIVKELLRRMTPEQMLPKMSQNASYHTPLTVVAVSGNMEIAEALVAKNPKLLEIPGINGQ 187

Query: 126 TPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELAFQLIH 185
            P+ +A     M+    LY        ++ ++ ++  +G  +   A+     ++A  L +
Sbjct: 188 IPVVVAVENTQMEMARYLY---TRTPVQVLLD-QDGYHGSLLFLNAIFYKMLDIALDLFN 247

Query: 186 MNND-AMSWVNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIETLSK 245
           M+   A++  ++    P+ VLAS P  F  G ++      +Y                  
Sbjct: 248 MSRRLAVTKHSQIESIPIIVLASKPDLFPGGCYLGPLTRFIY------------------ 307

Query: 246 AWTK-RLPKAKRIPYSFPDNYETCIEFFMTLKDAIFKGSKEIKKIVKKKQKHTWSVQVME 305
           +W + +LP   +   S  D   T                  ++K++K   K T       
Sbjct: 308 SWIQVKLPTLPQPSRSNRDQQNTL-----------------MRKLLKGLSKWT------- 367

Query: 306 KLFDQFGTPDKYGDDGKIPDGMATGHEDSETFPYTLEENKVNFSTNVVDKKQNDPEESKD 365
                 G  + Y         +   H  ++     + E  +              +E  +
Sbjct: 368 ------GIDEVY--------RLKVMHLQAKKLLLGISEETLALGL----------KERSE 427

Query: 366 VPDSAMLLAAKNG----VLEMVRGLSQRFPLAIHDTRNDKKNVL-LLAAEYRQPQVYRFL 425
             D A+L A + G    ++EM+R  S+     +  TR    + L LLA E+RQ +V+  L
Sbjct: 428 TVDEALLFAVRYGNVDFLVEMIRNNSE----LLWSTRTSSSSTLFLLAVEFRQEKVFSLL 487

Query: 426 HNKKDDLESLFRAVDVDGNGALHLAAAAS-KSMVWRITGDALQMQWEVKWYKFIEESMPL 485
           +   D    L    D DGNG LHLA   S  S +  + G  LQ+Q E++W+K +E   P 
Sbjct: 488 YGLDDRKYLLLADKDCDGNGVLHLAGFPSPPSKLSSVVGAPLQLQRELQWFKEVERIAPE 547

Query: 486 HFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTSKSCSVVGTLIVTVAFTSATTIPGG 545
                 N EE+T   IF + H  + +    W+  T+ SCS+V  LIVTV F +  T+PGG
Sbjct: 548 IEKERVNTEEQTPIEIFTKEHQGLRQEAEKWMKDTAMSCSLVAALIVTVTFAAVFTVPGG 607

Query: 546 YD--TEGVPFLEKEQSFFIFAVSSLIALCLSSASVTMFLAILTSRFEAKDFGSDLPWKLF 605
            D  ++G PF  +++ F IF VS LI+   S  SV +FL ILT+R+   DF   LP K+ 
Sbjct: 608 TDDNSKGKPFHLRDRRFIIFIVSDLISCFASCTSVLIFLGILTARYSFDDFLVFLPTKMI 652

Query: 606 IGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVALLLYTLTFLPVAFIFLISKLPLYIDVV 654
            GLS LF SI + LI+F +  + ++    + +         LP A +F++ + PL  +++
Sbjct: 668 AGLSILFVSIAAMLIAFSSALFTMMGKEGKWIVAPTILFACLP-ALLFVLLQYPLLKEMI 652

BLAST of Spg038038 vs. TAIR 10
Match: AT5G35810.1 (Ankyrin repeat family protein )

HSP 1 Score: 142.1 bits (357), Expect = 2.0e-33
Identity = 104/311 (33.44%), Postives = 174/311 (55.95%), Query Frame = 0

Query: 357 EESKDVPDSAMLL--AAKNGVLEMVRGLSQRFPLAIHDTRNDKKNVLLLAAEYRQPQVYR 416
           E S+ V  S MLL  AA++G LE++  L + +P  I    +  +++  +AA  R  +++ 
Sbjct: 23  EISQFVGSSPMLLFDAAQSGNLELLLILIRSYPDLIWTVDHKNQSLFHIAAINRHEKIFN 82

Query: 417 FLH--NKKDDLESLFRAVDVDGNGALHLAA-AASKSMVWRITGDALQMQWEVKWYKFIEE 476
            ++      DL ++++  + + N  LHL A     + +  ++G ALQMQ E+ WYK ++E
Sbjct: 83  RIYELGAIKDLIAMYKEKESNDN-LLHLVARLPPPNRLQVVSGAALQMQREILWYKAVKE 142

Query: 477 SMPLHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTSKSCSVVGTLIVTVAFTSATT 536
            +P  +    NK+E+ A  +F + H ++ K G  W+ +T+ +C +V TLI TV F +A T
Sbjct: 143 IVPRVYIKTKNKKEEVAHDLFTKEHDNLRKEGEKWMKETATACILVSTLIATVVFAAAFT 202

Query: 537 IPGGYDTE------GVPFLEKEQSFFIFAVSSLIALCLSSASVTMFLAILTSRFEAKDFG 596
           +PGG DT       G P   KE  F +F +S  +AL  S  S+ +FL+ILTSR+    F 
Sbjct: 203 LPGGNDTSGDIKTLGFPTFRKEFWFEVFIISDSVALLSSVTSIMIFLSILTSRYAEASFQ 262

Query: 597 SDLPWKLFIGLSSLFFSIVSTLISFCAGHYFLIDHRLR-SVALLLYTLTFLPVAFIFLIS 656
           + LP KL +GL +LF SI+S +++F A    + D   + S+ LL+Y  +   ++F+ L  
Sbjct: 263 TTLPTKLMLGLLALFVSIISMVLAFTATLILIRDQEPKWSLILLVYVASATALSFVVL-- 322

BLAST of Spg038038 vs. TAIR 10
Match: AT5G04680.1 (Ankyrin repeat family protein )

HSP 1 Score: 140.6 bits (353), Expect = 5.9e-33
Identity = 145/566 (25.62%), Postives = 245/566 (43.29%), Query Frame = 0

Query: 66  KLVKIICRNPGNEQLLE--TTNERNNNPLHLAAAQGSARMCHAIASANGRLVDMRNVDDE 125
           ++VK +      EQ+L   + N   +  L + A  G+  +  A+ + N +L+++  ++ +
Sbjct: 115 EIVKELLWRMTPEQMLPKMSQNVSYHTALTVVAVSGNMEIAEALVAKNPKLLEIPGINGQ 174

Query: 126 TPLFLAAAYGHMDAFYCLYYFCRNDSARIAINCREKNNGDTVLHRALRDDHFELAFQLIH 185
            P+ +A     M+    LY      +    +   +  +G  +   A+     ++A  L +
Sbjct: 175 IPVVVAVENTQMEMARYLY----TRTPVQVLLAEDGYHGTLLFLNAIFYRMLDIALDLFN 234

Query: 186 MNND-AMSWVNEKGLTPLHVLASYPTSFKSGSHIRGWQEIVYYCTCVDKLKPQPIETLSK 245
           M+   A++   +    P+ VLAS P  F    ++      +Y                  
Sbjct: 235 MSRRLAVTKHLQIESIPIIVLASKPDLFPGDCYLGPLTRFIY------------------ 294

Query: 246 AWTK-RLPKAKRIPYSFPDNYETCIEFFMTLKDAIFKGSKEIKKIVKKKQKHTWSVQVME 305
           +W + +LP   +  ++  D+           K +I+   K+++K        T   ++++
Sbjct: 295 SWIQVKLPTLPKPSHANKDHKSKFFRIHKVYKKSIYIPLKKVRKSF-DLFPDTLMRKLLK 354

Query: 306 KLFDQFGTPDKYGDDGKIPDGMATGHEDSETFPYTLEENKVNFSTNVVDKKQNDPEESKD 365
            L    G  + Y         +   H  ++     + E  +              +E  +
Sbjct: 355 GLSKWTGIDEVY--------RLKVMHLQAKKLLLGISEETLTLGL----------KERSE 414

Query: 366 VPDSAMLLAAKNG----VLEMVRGLSQRFPLAIHDTRNDKKNVL-LLAAEYRQPQVYRFL 425
             D A+L A + G    ++EM+R  S+     +  TR    + L LLA E+RQ +V+  L
Sbjct: 415 TVDEALLFAVRYGNVDFLVEMIRNNSE----LLWSTRTSSSSTLFLLAVEFRQEKVFNLL 474

Query: 426 HNKKDDLESLFRAVDVDGNGALHLAAAAS-KSMVWRITGDALQMQWEVKWYKFIEESMPL 485
           +   D    L    D DGNG LHLA   S  S +  +    L+MQ E++W+K +E   P 
Sbjct: 475 YGLDDRKYLLLADKDSDGNGVLHLAGFPSPPSKLASVICAPLRMQRELQWFKEVERIAPE 534

Query: 486 HFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTSKSCSVVGTLIV---------TVAF 545
                 N EE+T   IF + H  + +    W+  T+ SCS+V  LIV         TV F
Sbjct: 535 IEKERVNTEEQTPIEIFAKEHQGLRQEAEKWMKDTAMSCSLVAALIVMVTFAALIITVIF 594

Query: 546 TSATTIPGGYD--TEGVPFLEKEQSFFIFAVSSLIALCLSSASVTMFLAILTSRFEAKDF 605
            +  T+ GG D  +EG PF   EQ F IF VS LI+   +  +V +FL ILT+R+   DF
Sbjct: 595 AAVFTVSGGSDDNSEGNPFHLYEQRFIIFIVSDLISCFAACTAVPIFLGILTARYSFDDF 635

Query: 606 GSDLPWKLFIGLSSLFFSIVSTLISF 611
              LP K+  GLS LF SI + LI+F
Sbjct: 655 LVALPTKMITGLSILFVSIAAMLIAF 635

BLAST of Spg038038 vs. TAIR 10
Match: AT5G04730.1 (Ankyrin-repeat containing protein )

HSP 1 Score: 133.7 bits (335), Expect = 7.2e-31
Identity = 97/302 (32.12%), Postives = 154/302 (50.99%), Query Frame = 0

Query: 360 KDVPDSAMLLAAKNG----VLEMVRGLSQRFPLAIHDTRNDKKNVLLLAAEYRQPQVYRF 419
           K+    A+L AAK+G     +E+++  SQ   +         +N+  LA E+++ +++  
Sbjct: 291 KETVYEALLEAAKSGNRDFFIEIIKCNSQLLWIL---NPTSGRNLFQLAVEFKKEKIFNL 350

Query: 420 LHNKKDDLESLFRAVDVDGNGALHLAAAAS-KSMVWRITGDALQMQWEVKWYKFIEESMP 479
           +H   D   +L R+ D   N  LH+A   S    + +I+G AL+MQ E +W+K +E  + 
Sbjct: 351 IHGLDDRKVTLLRSYDKGNNNILHIAGRLSTPDQLSKISGAALKMQRESQWFKEVESLVS 410

Query: 480 LHFFTHYNKEEKTASTIFHETHMDMVKNGGDWLTQTSKSCSVVGTLIVTVAFTSATTIPG 539
                  NK+ KT   IF   H  + K G +W+  T+ +CS V  LI TV F +  T+PG
Sbjct: 411 EREVVQKNKDNKTPRQIFEHYHEHLRKEGEEWMKYTATACSFVAALIATVTFQAIFTVPG 470

Query: 540 GYD-TEGVPFLEKEQSFFIFAVSSLIALCLSSASVTMFLAILTSRFEAKDFGSDLPWKLF 599
           G D T G P +  +  F  F  +  +A   S  SV +FL+ILTSR+   DF   LP K+ 
Sbjct: 471 GIDGTSGSPLILNDLHFRAFIFTDTLAFFASCISVLIFLSILTSRYSFDDFIVSLPRKMI 530

Query: 600 IGLSSLFFSIVSTLISFCAGHYFLIDHRLRSVALLLYTLTFLP--VAFIFLISKLPLYID 654
           +G S LF SI S L++F       +   +R    L+Y L  L    + +FL+ + PL  +
Sbjct: 531 LGQSILFISIASMLVAFITS----LSASMRHKPALVYPLKPLASFPSLLFLMLQYPLLKE 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022995621.11.1e-25453.97uncharacterized protein LOC111491104 isoform X2 [Cucurbita maxima][more]
XP_022995622.13.2e-25454.13uncharacterized protein LOC111491104 isoform X3 [Cucurbita maxima][more]
XP_022995620.14.1e-25453.96uncharacterized protein LOC111491104 isoform X1 [Cucurbita maxima][more]
XP_023534305.12.4e-24651.52uncharacterized protein LOC111795904 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023534306.13.2e-24651.57uncharacterized protein LOC111795904 isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
B2RXR62.9e-0830.43Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Mu... [more]
Q014848.4e-0829.45Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=4[more]
Q8C8R38.4e-0829.45Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV=2[more]
Q8N8A24.2e-0730.86Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Ho... [more]
P161579.3e-0728.83Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1K2F15.2e-25553.97uncharacterized protein LOC111491104 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JZF81.5e-25454.13uncharacterized protein LOC111491104 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1K4G32.0e-25453.96uncharacterized protein LOC111491104 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1EX583.8e-24551.25uncharacterized protein LOC111437338 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1ESA56.4e-24551.19uncharacterized protein LOC111437338 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT3G18670.11.7e-4024.10Ankyrin repeat family protein [more]
AT5G04700.16.1e-3825.83Ankyrin repeat family protein [more]
AT5G35810.12.0e-3333.44Ankyrin repeat family protein [more]
AT5G04680.15.9e-3325.62Ankyrin repeat family protein [more]
AT5G04730.17.2e-3132.12Ankyrin-repeat containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 47..77
e-value: 0.12
score: 21.5
coord: 396..425
e-value: 380.0
score: 8.1
coord: 87..117
e-value: 21.0
score: 14.0
coord: 121..150
e-value: 150.0
score: 11.2
coord: 362..392
e-value: 4200.0
score: 0.3
coord: 161..190
e-value: 250.0
score: 9.5
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 47..69
score: 9.00344
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 291..509
e-value: 5.1E-9
score: 37.7
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 30..225
e-value: 4.1E-26
score: 93.6
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 41..444
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 52..139
e-value: 2.6E-7
score: 31.1
IPR041127Chlorophyllase enzymePFAMPF12740Chlorophyllase2coord: 699..812
e-value: 1.5E-13
score: 50.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 669..779
e-value: 7.1E-6
score: 27.4
IPR026961PGG domainPFAMPF13962PGGcoord: 505..616
e-value: 4.8E-24
score: 84.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 758..777
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 749..782
NoneNo IPR availablePANTHERPTHR24177:SF332REPEAT PROTEIN, PUTATIVE-RELATEDcoord: 39..665
NoneNo IPR availablePANTHERPTHR24177CASKINcoord: 39..665
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 47..69
score: 8.985412

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg038038.1Spg038038.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding