Spg037881 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg037881
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionBifunctional pinoresinol-lariciresinol reductase
Locationscaffold12: 41299597 .. 41302289 (+)
RNA-Seq ExpressionSpg037881
SyntenySpg037881
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGGCAATGGAGCCAGGAAGGGTGACATTTGATGATAAAATGGTGGTGAGAAAAGCCATTGAAGAGGCTCAAATTCCCTTCACTTACATCTCTGCAAACTGTTTTGCTGGTTACTTTCTTGGTGGTTTGTGTCAACCTGGTTCAATCCTTCCCTCCAAAGACCATGTTCTCCTCCTTGGAGATGGCAATCAAAAAGGTTCTATACATATACACATACATACATATATACGTACGCATATCGGTTATTTTACATATTATAGTTCAAACAACAACGAGTTAGGTAATGCGATTGAGTTTTGTTCAAATCGGCTAGACATATGGCGTTCACATGAGATTTCTTTTTATTTTTGTGTTTTTATTTTAGGTTAAATCATTAAAAATACCCCTGGATTGTTAAGTTCGTTTCAATTTTACTCCTAAACTTTCAAAAGTTTCATTTTAGCTCGTGAAGTTTGAAATTTGTTTCAAAACAGACCTTGAAGGCATTTTTCGTTAAATTCATAAATAGAAAATGACATTTTTGTATATGTATTGTTGATTATGTGAACAGATTTGAACATATACATGGAAAAAACAATTAAAAAAAACACATGCTAACACATCATTTTTCGTCCATAAATTTCACAAAAATAGAGTCGTCGAACTTTTAGGGCCCGTTTGATAACCATTCCGTTTTCTGTTTTCTGTTTTTGTTTTTCATTTTTTAAAAACAAGGGTGTTTGATAACCATTTCTGTTTTCTGTTTTCTGTTTTTTAAAAACGTTTTTGAAATTATAAGTAAATTTCAAAAACCAAAAAAAGTAGTTTTTAAAAACAGATTTCTGTTTTTTCAAAAACTCATTTTTCTTCTAATGCTTACTTTTCTCCTCTTTTACCTCTCTCTCCAACAAATTTATTTCTCTATTTTTTTTTTCTCTCTAAAATTCTCTCTCAAAACTTTTTTTTTCTCTATAACTTGTCTATTTCTAAACACTTGTCAATACCTTTTCTCTCCCCAATAAACATGTTCTAATTTTTCTTTTGGAGAAAAAAAAGTCACCAAAATTTTCTATCGCTACAACTTTTCTTTATTTTTCTAATTTATCGTTAGTTCTTTAGCATATCTTTTACTCTTAACTTTACTATCTCTCTAAATTTTCTTTTTTTATTTAATTTTTTAATCTAAAATTTATCGCTTTTACTCTAACTTGTTTTCTATTTTTTCTCTAACTAATATCTTTTCTCGAGTCTAACTAATATTCTTTCTCTAAACATTTTCTTTCATCTCTCTAAATTTTCTATGTTTAACTTTTTCTCTCGTCTTTTCAAAAAAAAAAAAAAAAATCTCTTGAAAACATTTATATTTGTGTCTCTAAATTTTTGTTTCAACGTCTTTACACATTTTTCTCTCTCTAAAATATCTTTTCATCTTTCTAATTTTTAAAAAACTGAAAACCAAAAACAGTTATCAAATAAGGTTGGTTTTCTGTTTTTTAAAAACAGAAAACAAAAAACAGTTATCAAACATATGTGGTTTTTGTTTTTAAAAAACAGGAAACCAAAAACCAAAAACCAAAAACAGAAAAACCAAAAACCAAAACTGAAATGGTTATCAAACGGGCCCTTAACTTCTTATATTGACATTTTTTGTGGTACGTTAATGGTGACAGCAATATATGTGGATGAAGATGACATTGCAATGTACACCATCAAGACCATTGATGACCCTCGAACTCTAAACAAAACGGTGTACATTAGGCCACCCAAGAACATATTATCACAAAGAGAAGTTGTTGAAATTTGGGAGAAACTAATTGGAAAACAACTCACAAAGAGCACCATTTCTGATCAAGAGTTTCTAGCTAATATGAAAGGTAAGTTCATCTATTTACGTATCTATGATTATAGGAATACATTTTAGTCTCTAAAGTTTCGGTGTACTATTAAGTTAACCACTCTGACTAATTTCAAAATTTTCAATTTAGCCTCCTATGTTTAATATTTTTTTTTCTATTGGTGAATGTTGAATTGACTGGAGATACTTAGTGAGTTGAGTAATTAAAAACTTTCATCTCTTAAGCGACTTAATTCATCCTCACTAAGTATGTGCCATGTAAGTTTACCATCAGTCAATTCTAACATTCGCCATTGAAAAGGGTATCATTGAAAAATGTTAAAACATAAAGGATTAAGTTGAAATAACATCAAATTAGAAACTAAAACATGTTTAGCCTTCTTTTTCCTTGCATGTGAAAACTTTGATTGGAGATTGAATTGACTTGGTTTTTTTTTTTTTTTTTTTTTTTGGGAAATCAAAATCATTTCTGAAAAAAAAAAAAAAAAAAAAAGGTCAAGATTACGCAGAGCAAGTTGGGTTAAGTCACTATTATCATGTATGCTACGAGGGTTGTCTTGCAAATTTCGAAATAGGAGAGGATGCTGAAGAAGCTTGCAATCTCTATCCAGAAGTCAACTACACAACTGTGGAGGAATACATGAAACGTTATGTTTAGGGGGCAATATGCAAACTCTAAAAATAAATCATTTGGGTTACAATTCAAGAGACTTGAATAAAAAGTTTCCTTCCACTTTGTGTTGTACATTAGTCAACATTGACTCGTGTCCCAAATTCTTATGTGCTATTTAAGTATTTATAACCAATATCAATGTTGAAAAAACCCATTTGATTGTATTATCGAGTGTTTAAC

mRNA sequence

ATGGAGAAGGCAATGGAGCCAGGAAGGGTGACATTTGATGATAAAATGGTGGTGAGAAAAGCCATTGAAGAGGCTCAAATTCCCTTCACTTACATCTCTGCAAACTGTTTTGCTGGTTACTTTCTTGGTGCAATATATGTGGATGAAGATGACATTGCAATGTACACCATCAAGACCATTGATGACCCTCGAACTCTAAACAAAACGGTGTACATTAGGCCACCCAAGAACATATTATCACAAAGAGAAGTTGTTGAAATTTGGGAGAAACTAATTGGAAAACAACTCACAAAGAGCACCATTTCTGATCAAGAGTTTCTAGCTAATATGAAAGGTCAAGATTACGCAGAGCAAGTTGGGTTAAGTCACTATTATCATGTATGCTACGAGGGTTGTCTTGCAAATTTCGAAATAGGAGAGGATGCTGAAGAAGCTTGCAATCTCTATCCAGAAGTCAACTACACAACTGTGGAGGAATACATGAAACGTTATGTTTAG

Coding sequence (CDS)

ATGGAGAAGGCAATGGAGCCAGGAAGGGTGACATTTGATGATAAAATGGTGGTGAGAAAAGCCATTGAAGAGGCTCAAATTCCCTTCACTTACATCTCTGCAAACTGTTTTGCTGGTTACTTTCTTGGTGCAATATATGTGGATGAAGATGACATTGCAATGTACACCATCAAGACCATTGATGACCCTCGAACTCTAAACAAAACGGTGTACATTAGGCCACCCAAGAACATATTATCACAAAGAGAAGTTGTTGAAATTTGGGAGAAACTAATTGGAAAACAACTCACAAAGAGCACCATTTCTGATCAAGAGTTTCTAGCTAATATGAAAGGTCAAGATTACGCAGAGCAAGTTGGGTTAAGTCACTATTATCATGTATGCTACGAGGGTTGTCTTGCAAATTTCGAAATAGGAGAGGATGCTGAAGAAGCTTGCAATCTCTATCCAGAAGTCAACTACACAACTGTGGAGGAATACATGAAACGTTATGTTTAG

Protein sequence

MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLGAIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQLTKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTTVEEYMKRYV
Homology
BLAST of Spg037881 vs. NCBI nr
Match: XP_008460436.1 (PREDICTED: bifunctional pinoresinol-lariciresinol reductase 2-like [Cucumis melo] >KAA0068133.1 bifunctional pinoresinol-lariciresinol reductase 2-like [Cucumis melo var. makuwa] >TYK21053.1 bifunctional pinoresinol-lariciresinol reductase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 309.3 bits (791), Expect = 2.0e-80
Identity = 155/189 (82.01%), Postives = 162/189 (85.71%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           MEKAMEPGRVTFDDKMVVRKAIEEA+IPFTYISANCFAGYFLG                 
Sbjct: 125 MEKAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGLILPSKDHVLL 184

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                  AIYVDEDDIAMYT+K+IDD RTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL
Sbjct: 185 LGDGNQKAIYVDEDDIAMYTMKSIDDSRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 244

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
           TK++IS QEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIG+D EEACNLYPEVNYTT
Sbjct: 245 TKTSISSQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGKDGEEACNLYPEVNYTT 304

BLAST of Spg037881 vs. NCBI nr
Match: XP_038888404.1 (bifunctional pinoresinol-lariciresinol reductase 2-like [Benincasa hispida])

HSP 1 Score: 303.9 bits (777), Expect = 8.4e-79
Identity = 151/189 (79.89%), Postives = 161/189 (85.19%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           ME AMEPGRVTFDDKMVVRKAIEEA+IPFTYISANCFAGYFLG                 
Sbjct: 124 MENAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHVVL 183

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                  AIYVDEDDIAMYT+KTIDDPRTLNKTVYIRPPKN LSQREVV+IWEKLIGKQL
Sbjct: 184 LGDGNQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNSLSQREVVDIWEKLIGKQL 243

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
           TK++IS QEFLANMKGQ+YAEQVGL+HYYHVCYEGCLANFEIG+D EEACNLYPEVNYT+
Sbjct: 244 TKTSISAQEFLANMKGQNYAEQVGLTHYYHVCYEGCLANFEIGKDGEEACNLYPEVNYTS 303

BLAST of Spg037881 vs. NCBI nr
Match: XP_022963982.1 (bifunctional pinoresinol-lariciresinol reductase 2-like [Cucurbita moschata])

HSP 1 Score: 302.4 bits (773), Expect = 2.4e-78
Identity = 151/189 (79.89%), Postives = 162/189 (85.71%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           M+ AMEPGRVTFDDKMVVRKAIEEA+IPFTYISANCFAGYFLG                 
Sbjct: 124 MKNAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGFILPSKHHVSL 183

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                  AIYVDEDDIAMYT+KTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGK+L
Sbjct: 184 LGDGNQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKEL 243

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
           TKS+ISDQEFLANMKGQ YAEQVGL+HYYHVC+EGCLANFEIG+ AEEAC+LYPEV+YT+
Sbjct: 244 TKSSISDQEFLANMKGQGYAEQVGLTHYYHVCFEGCLANFEIGDGAEEACSLYPEVSYTS 303

BLAST of Spg037881 vs. NCBI nr
Match: XP_023554706.1 (bifunctional pinoresinol-lariciresinol reductase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298.5 bits (763), Expect = 3.5e-77
Identity = 149/189 (78.84%), Postives = 161/189 (85.19%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           M+ AMEPGRVTFDDKMVVRKAIEEA+IPFTYISANCFAGYFLG                 
Sbjct: 124 MKNAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGFILPSKHHVSL 183

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                  AIYVDEDDIA YT+KTIDDPRTLNKTVYIRPP+NILSQREVVEIWEKLIGK+L
Sbjct: 184 LGDGNQKAIYVDEDDIATYTMKTIDDPRTLNKTVYIRPPENILSQREVVEIWEKLIGKEL 243

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
           TKS+ISDQEFLANMKGQ YAEQVGL+HYYHVC+EGCLANFEIG+ AEEAC+LYPEV+YT+
Sbjct: 244 TKSSISDQEFLANMKGQGYAEQVGLTHYYHVCFEGCLANFEIGDGAEEACSLYPEVSYTS 303

BLAST of Spg037881 vs. NCBI nr
Match: KAG6571622.1 (Bifunctional pinoresinol-lariciresinol reductase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 298.1 bits (762), Expect = 4.6e-77
Identity = 150/189 (79.37%), Postives = 160/189 (84.66%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           M+ AMEPGRVTFDDKMVVRKAIEEA+IPFTYISANCFAGYFLG                 
Sbjct: 124 MKNAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGFILPSKHHVSL 183

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                  AIYVDEDDIAMYT+KTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGK+L
Sbjct: 184 LGDGNQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKEL 243

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
           TKS+ISDQEFLANMKGQ YAEQVGL+HYYHVC+EG LANFEIG+ AEEAC+LYPEV YT+
Sbjct: 244 TKSSISDQEFLANMKGQGYAEQVGLTHYYHVCFEGVLANFEIGDGAEEACSLYPEVGYTS 303

BLAST of Spg037881 vs. ExPASy Swiss-Prot
Match: E6Y2X0 (Bifunctional pinoresinol-lariciresinol reductase 2 OS=Linum usitatissimum OX=4006 GN=PLR_Lu2 PE=1 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 7.1e-73
Identity = 139/189 (73.54%), Postives = 149/189 (78.84%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           ME AMEPGRVTFDDKMVVRKAIEEA IPFTYISANCFAGYFLG                 
Sbjct: 142 MENAMEPGRVTFDDKMVVRKAIEEAGIPFTYISANCFAGYFLGGLCQPGFILPSREQVTL 201

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                  A+YVDEDDIA YTIK IDDPRTLNKTVYI+PPKN+LSQREVV IWEK IGK+L
Sbjct: 202 LGDGNQKAVYVDEDDIARYTIKMIDDPRTLNKTVYIKPPKNVLSQREVVGIWEKYIGKEL 261

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
            K+T+S +EFLA MK QDYAEQVGL+HYYHVCYEGCL NFEIG++A EA  LYPEV YTT
Sbjct: 262 KKTTLSVEEFLAMMKEQDYAEQVGLTHYYHVCYEGCLTNFEIGDEAGEATKLYPEVGYTT 321

BLAST of Spg037881 vs. ExPASy Swiss-Prot
Match: Q4R0I0 (Bifunctional pinoresinol-lariciresinol reductase OS=Linum album OX=191219 GN=PLR1 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 3.1e-68
Identity = 124/189 (65.61%), Postives = 149/189 (78.84%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           ME AMEPGR+TFDDKMVVR+AIEEA IPFTY+SANCFAGYFLG                 
Sbjct: 139 MENAMEPGRITFDDKMVVRRAIEEAGIPFTYVSANCFAGYFLGGLCQPGYILPSRDHVTL 198

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                   +YVDEDD A YT++ IDDPRTLNKT+Y++PPKN+LSQREVV IWEK IGK+L
Sbjct: 199 LGDGDKKGVYVDEDDTAAYTLRAIDDPRTLNKTIYVKPPKNVLSQREVVGIWEKYIGKEL 258

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
            K+ +S+Q+FLA M+ Q+YAEQVGL+HYYHVCYEGCL+NFE+ +D +EA  LYP+V+YTT
Sbjct: 259 QKTILSEQDFLATMREQNYAEQVGLTHYYHVCYEGCLSNFEV-DDEQEASKLYPDVHYTT 318

BLAST of Spg037881 vs. ExPASy Swiss-Prot
Match: B5KRH5 (Bifunctional pinoresinol-lariciresinol reductase OS=Linum corymbulosum OX=480358 GN=PLR_Lc1 PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 6.9e-68
Identity = 129/189 (68.25%), Postives = 147/189 (77.78%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           M  AMEPGRVTFDDKMVVR+AIEEA IPFTY+SANCFAGYFLG                 
Sbjct: 128 MGDAMEPGRVTFDDKMVVRRAIEEAAIPFTYVSANCFAGYFLGGLCQPGSILPSRDHVTL 187

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                   +YVDE+DIA YT+K IDDPRTLNKT+YI+PPKNILSQR+VV IWEK IGKQL
Sbjct: 188 LGDGNQKGVYVDENDIAAYTLKAIDDPRTLNKTLYIKPPKNILSQRQVVGIWEKHIGKQL 247

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
            K+ +S+Q+FLA MK QDYAEQVGL+HYYHVCYEGCL NFE+ +D +EA  LYP+V YTT
Sbjct: 248 HKTLLSEQDFLAAMKEQDYAEQVGLTHYYHVCYEGCLTNFEVEQD-QEASKLYPDVRYTT 307

BLAST of Spg037881 vs. ExPASy Swiss-Prot
Match: P52581 (Isoflavone reductase homolog OS=Lupinus albus OX=3870 PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.0e-63
Identity = 116/189 (61.38%), Postives = 144/189 (76.19%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           M  A+EPGRVTFD+KM VRKAIEEA IPFTYISANCFAGYF G                 
Sbjct: 124 MGHALEPGRVTFDEKMTVRKAIEEANIPFTYISANCFAGYFAGNLSQMKTLLPPRDKVLL 183

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                   +Y+DEDD+A YTIKTIDDPRTLNKTVY+RPP+NIL+ +E++E WE+LIGKQL
Sbjct: 184 YGDGNVKPVYMDEDDVATYTIKTIDDPRTLNKTVYLRPPENILTHKELIEKWEELIGKQL 243

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
            K++IS+++FL+ +KG D+A QVG+ H+YH+ YEGCL NFEIGE+ EEA  LYPEVNYT 
Sbjct: 244 EKNSISEKDFLSTLKGLDFASQVGVGHFYHIFYEGCLTNFEIGENGEEASELYPEVNYTR 303

BLAST of Spg037881 vs. ExPASy Swiss-Prot
Match: B0LL23 (Bifunctional pinoresinol-lariciresinol reductase OS=Sinopodophyllum hexandrum OX=93608 GN=PLR PE=1 SV=2)

HSP 1 Score: 242.3 bits (617), Expect = 3.9e-63
Identity = 123/189 (65.08%), Postives = 140/189 (74.07%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           M  AMEPGR TFD+KMVVRKAIE+A+IP TY SANCFAGYFLG                 
Sbjct: 124 MAHAMEPGRATFDEKMVVRKAIEDAKIPHTYASANCFAGYFLGGLCQFGKIIPSKESVIL 183

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                   IYVDE DIA YTIKT+DDPRTLNKT+YIRPP NILSQREVVEIWEKLIGK L
Sbjct: 184 SGDGNVKGIYVDEYDIATYTIKTMDDPRTLNKTIYIRPPANILSQREVVEIWEKLIGKVL 243

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
            KS++S+++FLA MKG  +  Q GL+HYYHV YEGCL NFE+ ED  +A  LYP+VNYTT
Sbjct: 244 DKSSLSEEDFLALMKGLSHGHQAGLTHYYHVSYEGCLTNFEV-EDGVDASKLYPQVNYTT 303

BLAST of Spg037881 vs. ExPASy TrEMBL
Match: A0A5A7VJ49 (Bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold392G00120 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 9.7e-81
Identity = 155/189 (82.01%), Postives = 162/189 (85.71%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           MEKAMEPGRVTFDDKMVVRKAIEEA+IPFTYISANCFAGYFLG                 
Sbjct: 125 MEKAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGLILPSKDHVLL 184

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                  AIYVDEDDIAMYT+K+IDD RTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL
Sbjct: 185 LGDGNQKAIYVDEDDIAMYTMKSIDDSRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 244

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
           TK++IS QEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIG+D EEACNLYPEVNYTT
Sbjct: 245 TKTSISSQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGKDGEEACNLYPEVNYTT 304

BLAST of Spg037881 vs. ExPASy TrEMBL
Match: A0A1S3CCK4 (bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucumis melo OX=3656 GN=LOC103499245 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 9.7e-81
Identity = 155/189 (82.01%), Postives = 162/189 (85.71%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           MEKAMEPGRVTFDDKMVVRKAIEEA+IPFTYISANCFAGYFLG                 
Sbjct: 125 MEKAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGLILPSKDHVLL 184

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                  AIYVDEDDIAMYT+K+IDD RTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL
Sbjct: 185 LGDGNQKAIYVDEDDIAMYTMKSIDDSRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 244

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
           TK++IS QEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIG+D EEACNLYPEVNYTT
Sbjct: 245 TKTSISSQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGKDGEEACNLYPEVNYTT 304

BLAST of Spg037881 vs. ExPASy TrEMBL
Match: A0A6J1HGM2 (bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464117 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.2e-78
Identity = 151/189 (79.89%), Postives = 162/189 (85.71%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           M+ AMEPGRVTFDDKMVVRKAIEEA+IPFTYISANCFAGYFLG                 
Sbjct: 124 MKNAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGFILPSKHHVSL 183

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                  AIYVDEDDIAMYT+KTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGK+L
Sbjct: 184 LGDGNQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKEL 243

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
           TKS+ISDQEFLANMKGQ YAEQVGL+HYYHVC+EGCLANFEIG+ AEEAC+LYPEV+YT+
Sbjct: 244 TKSSISDQEFLANMKGQGYAEQVGLTHYYHVCFEGCLANFEIGDGAEEACSLYPEVSYTS 303

BLAST of Spg037881 vs. ExPASy TrEMBL
Match: A0A6J1HRL4 (bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111466859 PE=4 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.2e-77
Identity = 149/189 (78.84%), Postives = 159/189 (84.13%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           M+ AMEPGRVTFDDKMVVRKAIEEA+IPFTYISANCFAGYFLG                 
Sbjct: 124 MKNAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGFILPSKHHVSL 183

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                  AIYVDEDDIAMYT+ TIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGK+L
Sbjct: 184 LGDGNKKAIYVDEDDIAMYTMNTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKEL 243

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
            KS+ISDQEFLANMKGQ YAEQVGL+HYYHVC+EGCLANFEIG+ AEEAC LYPEV+YT+
Sbjct: 244 AKSSISDQEFLANMKGQGYAEQVGLTHYYHVCFEGCLANFEIGDGAEEACRLYPEVSYTS 303

BLAST of Spg037881 vs. ExPASy TrEMBL
Match: A0A0A0L8W5 (NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G636980 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.0e-77
Identity = 150/189 (79.37%), Postives = 158/189 (83.60%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           ME AMEPGRVTFDDKMVVRKAIEEA+IPFTYISANCFAGYFLG                 
Sbjct: 124 MEDAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHVLL 183

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                  AIYVDEDDIAMYT+K+IDD RTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL
Sbjct: 184 LGDGNQKAIYVDEDDIAMYTMKSIDDCRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 243

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
            K++IS QEFLANMK QDYA QVGLSHYYHVCYEGCLANFEIG+D EEACNLYPEV+YTT
Sbjct: 244 IKTSISSQEFLANMKDQDYAAQVGLSHYYHVCYEGCLANFEIGKDGEEACNLYPEVDYTT 303

BLAST of Spg037881 vs. TAIR 10
Match: AT1G32100.1 (pinoresinol reductase 1 )

HSP 1 Score: 189.1 bits (479), Expect = 2.8e-48
Identity = 92/189 (48.68%), Postives = 125/189 (66.14%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           M  A+ PGR TFD KM VR+AIE A IP+TY+   CFA YF G                 
Sbjct: 130 MGHALPPGRETFDQKMEVRQAIEAAGIPYTYVVGACFAAYFAGNLSQMVTLLPPKEKVNI 189

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                   ++ DEDDIA YT KT++DPRTLNKTV IRPP N+L+Q E+V+IWEKL GK+L
Sbjct: 190 YGDGNVKVVFADEDDIAKYTAKTLNDPRTLNKTVNIRPPDNVLTQLELVQIWEKLTGKEL 249

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
            K+ I+ Q+FLAN++  +   Q G+ H+YH+ YEGCL + E+GED EEA +LYP+V Y  
Sbjct: 250 EKTNIAAQDFLANIEQMEIPHQAGIGHFYHIFYEGCLTDHEVGED-EEASSLYPDVKYKR 309

BLAST of Spg037881 vs. TAIR 10
Match: AT4G13660.1 (pinoresinol reductase 2 )

HSP 1 Score: 177.6 bits (449), Expect = 8.4e-45
Identity = 89/189 (47.09%), Postives = 121/189 (64.02%), Query Frame = 0

Query: 1   MEKAMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFLG----------------- 60
           M  AM PG  TFD KM +R AI+ A I  TY+   CFA YF G                 
Sbjct: 130 MGHAMPPGSETFDQKMEIRNAIKAAGISHTYLVGACFAAYFGGNLSQMGTLFPPKNKVDI 189

Query: 61  -------AIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 120
                   ++VDEDD+A YT KT++DPRTLNKTVY+RP  NIL+Q E+V+IWEKL  K+L
Sbjct: 190 YGDGNVKVVFVDEDDMAKYTAKTLNDPRTLNKTVYVRPTDNILTQMELVQIWEKLTEKEL 249

Query: 121 TKSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGEDAEEACNLYPEVNYTT 166
            K+ +S  +FLA+++ ++ + Q GL H+YH+ YEGCL + E+G+D EEA  LYP+V Y  
Sbjct: 250 EKTYVSGNDFLADIEDKEISHQAGLGHFYHIYYEGCLTDHEVGDD-EEATKLYPDVKYKR 309

BLAST of Spg037881 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 1.4e-28
Identity = 70/188 (37.23%), Postives = 100/188 (53.19%), Query Frame = 0

Query: 4   AMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFL--------------------- 63
           A+EP +  F  K+  R+ IE   IP+TY+  N FAGY+L                     
Sbjct: 123 AVEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIF 182

Query: 64  -----GAIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQLT 123
                 A+   E+DIA YTIK +DDPRTLNKT+YI PP N LS  E+V +WEK IGK + 
Sbjct: 183 GDGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKSVE 242

Query: 124 KSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGED-AEEACNLYPEVNYTT 165
           K  +S+++   +++       V LS  + V  +G   NF I      EA  LYP++ YT+
Sbjct: 243 KIYMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKYTS 302

BLAST of Spg037881 vs. TAIR 10
Match: AT4G39230.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 118.2 bits (295), Expect = 6.1e-27
Identity = 69/186 (37.10%), Postives = 97/186 (52.15%), Query Frame = 0

Query: 5   MEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFL---------------------- 64
           +EP +  +  K  +R+ IE   IP+TY+S N FAGYFL                      
Sbjct: 123 VEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLGDG 182

Query: 65  --GAIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQLTKST 124
              A++  E+DI  YTI  +DDPRTLNK +YIRPP N  S  ++V +WE  IGK L +  
Sbjct: 183 NPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLERIY 242

Query: 125 ISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGED-AEEACNLYPEVNYTTVEE 166
           + +++ L  +        V LS  + V  +G   +FEI      EA  LYP+V YTTV+E
Sbjct: 243 VPEEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTVDE 302

BLAST of Spg037881 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 117.1 bits (292), Expect = 1.4e-26
Identity = 69/188 (36.70%), Postives = 97/188 (51.60%), Query Frame = 0

Query: 4   AMEPGRVTFDDKMVVRKAIEEAQIPFTYISANCFAGYFL--------------------- 63
           A+EP +  F  K+ +R+ IE   IP+TY    CF GY+L                     
Sbjct: 123 AVEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTIL 182

Query: 64  -----GAIYVDEDDIAMYTIKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQLT 123
                 A+   E+DIA YTIK +DDPRTLNK +YI+P  N LS  E+V +WEK IGK L 
Sbjct: 183 GDGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLE 242

Query: 124 KSTISDQEFLANMKGQDYAEQVGLSHYYHVCYEGCLANFEIGED-AEEACNLYPEVNYTT 165
           K+ + +++ L +++       V LS  + V   G   N  I      EA  LYP+V YT+
Sbjct: 243 KTHLPEEQLLKSIQESPIPINVVLSINHAVFVNGD-TNISIEPSFGVEASELYPDVKYTS 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008460436.12.0e-8082.01PREDICTED: bifunctional pinoresinol-lariciresinol reductase 2-like [Cucumis melo... [more]
XP_038888404.18.4e-7979.89bifunctional pinoresinol-lariciresinol reductase 2-like [Benincasa hispida][more]
XP_022963982.12.4e-7879.89bifunctional pinoresinol-lariciresinol reductase 2-like [Cucurbita moschata][more]
XP_023554706.13.5e-7778.84bifunctional pinoresinol-lariciresinol reductase 2-like [Cucurbita pepo subsp. p... [more]
KAG6571622.14.6e-7779.37Bifunctional pinoresinol-lariciresinol reductase 2, partial [Cucurbita argyrospe... [more]
Match NameE-valueIdentityDescription
E6Y2X07.1e-7373.54Bifunctional pinoresinol-lariciresinol reductase 2 OS=Linum usitatissimum OX=400... [more]
Q4R0I03.1e-6865.61Bifunctional pinoresinol-lariciresinol reductase OS=Linum album OX=191219 GN=PLR... [more]
B5KRH56.9e-6868.25Bifunctional pinoresinol-lariciresinol reductase OS=Linum corymbulosum OX=480358... [more]
P525811.0e-6361.38Isoflavone reductase homolog OS=Lupinus albus OX=3870 PE=2 SV=1[more]
B0LL233.9e-6365.08Bifunctional pinoresinol-lariciresinol reductase OS=Sinopodophyllum hexandrum OX... [more]
Match NameE-valueIdentityDescription
A0A5A7VJ499.7e-8182.01Bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucumis melo var. mak... [more]
A0A1S3CCK49.7e-8182.01bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucumis melo OX=3656 ... [more]
A0A6J1HGM21.2e-7879.89bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucurbita moschata OX... [more]
A0A6J1HRL42.2e-7778.84bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucurbita maxima OX=3... [more]
A0A0A0L8W55.0e-7779.37NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G636980 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT1G32100.12.8e-4848.68pinoresinol reductase 1 [more]
AT4G13660.18.4e-4547.09pinoresinol reductase 2 [more]
AT1G75290.11.4e-2837.23NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G39230.16.1e-2737.10NmrA-like negative transcriptional regulator family protein [more]
AT1G75280.11.4e-2636.70NmrA-like negative transcriptional regulator family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008030NmrA-like domainPFAMPF05368NmrAcoord: 46..154
e-value: 6.7E-13
score: 48.7
coord: 3..45
e-value: 5.8E-8
score: 32.5
NoneNo IPR availableGENE3D3.90.25.10coord: 45..161
e-value: 9.1E-46
score: 158.2
NoneNo IPR availableGENE3D3.90.25.10coord: 34..44
e-value: 8.5E-12
score: 46.9
NoneNo IPR availableGENE3D3.40.50.720coord: 50..150
e-value: 9.1E-46
score: 158.2
NoneNo IPR availableGENE3D3.40.50.720coord: 3..33
e-value: 8.5E-12
score: 46.9
NoneNo IPR availablePANTHERPTHR43349PINORESINOL REDUCTASE-RELATEDcoord: 44..164
NoneNo IPR availablePANTHERPTHR43349:SF47BIFUNCTIONAL PINORESINOL-LARICIRESINOL REDUCTASE-LIKEcoord: 1..44
NoneNo IPR availablePANTHERPTHR43349:SF47BIFUNCTIONAL PINORESINOL-LARICIRESINOL REDUCTASE-LIKEcoord: 44..164
NoneNo IPR availablePANTHERPTHR43349PINORESINOL REDUCTASE-RELATEDcoord: 1..44
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 4..119

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg037881.1Spg037881.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009807 lignan biosynthetic process
molecular_function GO:0016491 oxidoreductase activity