Spg036901 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg036901
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionprotein kinase 2B, chloroplastic-like
Locationscaffold5: 45917210 .. 45920429 (-)
RNA-Seq ExpressionSpg036901
SyntenySpg036901
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGAGACCAGGTGTCTGTAAGATTGAACGGGTCTAACTTTTTTTTCCCTCTATATTTTTTGAAACTGAATGGGTCTTTCTCAACTGTATCTGTATCCCTTTTCTCTCTATCGTGGTGACCTTGTGTTTGTAAATACAATGCAATAATTACTTAAATAATCAAGCTTCTGCATTAATTGCTGATTGTCAGCATAAATAGGATTAGTTTAGTTTGTCACGTCCTTACTTCATCATCAAGTCATTGTCACTCTTATCCCATGCAGAAGAACCGAAAAATCATGATAGGAAAGGCCATTCATCTGCTTCTTTGAGAGCTCCATCACTTGGTGGGAATACTCCTTCTAGAGGTCTTCAAGCTCATAGATCCGAAGGTGAAATATTGTTGTCTCCAAATCTGAAAGCCTTCACATACAGTGAGCTTAAGAATGCTACTAAAAACTTCCGCCCTGACAGTCTCATTGGAGAAGGAGGTTTTGGTCATGTTTACAAAGGATGGATTGATGAACATACACTTGAAGCTGTGCGGCCTGGAGCTGGAATGGTTGTTGCTGTCAAGAAGCTTAAACCTGAAGGTTTTCAAGGGCACAAAGAATGGCTGGTAGTTTTCTTCTTCTTTTTGATATGTCCCATTGATCTTTCTGGCTTAATAGTTTGCTGTATAAATCAGGTTGCTATTGATTGCAGTCTGAAGTAAATTACCTCGGTCAGCTTCATCATCCAAATCTTGTTAAACTAATCGGATTCTGCCTTGATGGAGATAGCCGACTTTTGGTCTATGAGTACATGTCCAAAGGCAGCCTTGAAAATCATCTATTTAGAAGTAAGTCAAATGTTTTCGCTAACTGTAGCTGAAAGCCCACTGCAACCATTAACCTCGGTTCTTGAATGTTCAATGACATTGATTTTTTCTTAATTATATATGTCATTACAATAATTCAGGGGGCGCACGTCCACTTTCTTGGGCAATAAGGATCAAAGTTGCTATAGGTGCTGCTCGAGGGCTTTCCTTTCTGCATGACTCTGAAGAACCAGTTATCTACCGCGATTTTAAGGCATCAAACATCCTTTTAGATTCTGTATGTCATTTAAGTCCTTCATAGCAAAGACTTCTGTAGAATCTTATTTAGATCTTGTGAAAATCAAAATGGGTACAAGTAAATGTAATGCATATGAAAAAGTGACGTAAAAGTAAGTAGAGAGAAGATAAAAACACTTTTCTAATCTATTAAATACAAAGAACCAAGAATTTAGGTCACCAGCACAAGCTATTGTGAAAATTCACCAGTCTGACTAGTAAGTATGAAATTGTTTTGTTCACAGGAATTCAACGCGAAACTTTCAGATTTTGGTTTGGCAAAAGCAGGCCCAACTGGCGATCGGACTCATGTGTCCACTCAAGTCATGGGTACTCAAGGCTATGCTGCTCCAGAATATATTGCCACTGGTTTGTTTTCTGCCCCACCTTAAGAATCTTTGTTTTTTTCTGCAAGTAATTGTTGTAGATGACCTGTGACCCTCCAAATTATTTTTGCTGATTCCTTCTAGAGAAAGGAATAGGTTATAATTTGCATAAATATGTTTTGAGTTTATTCTTGATATCAATCTGATATGTGTAATTATTGTAGAATTGTTGTAGGATATGTGCAATTTGAAGTTTGATCTAGGCATTGGTTGATGGGCATTCTAGATCGTGGGATGTCTAGAATTCATTCCCCTCGCCCCAATATTTATTCTCATAATTACTCAACATCCTGATAGATACATTGAGCTACCAAACTTAGTAGTTCGACTTTTTTTTGTCTATCTTCTGTTTCAACTCATCACTAAATTTCAAATCTGCCAGTTTGTCTGGTAGATGAAATTTGATTGTAATAAATCCACTTAAAGGGAAATCATTATTGCTTTTTATTTTGATGTGTGAATTGGTTGGCAAATCTTGAGAAGTTTTCCAGCTACTTGGATAAGATTAGCAACCACTGAGCATGAAACCAGTGTAACTATGGATTTCTAAATGTCTGGTCTTGAACTAAAGAGCTTAGGGACCAGGCAAAGCATGTTCATTTCAGAGTTTCCCATTAGATATTACATTTCTAAAGTTGTATTGTGGTAGTTTATAGATATACCTCTATCCTTCCCATTGCAGGTAGGCTAACGGCCAAGTGTGACGTTTATAGCTTTGGGGTTGTGCTGCTGGAGCTCCTCTCTGGCCGCCGTGCTGTCGATAAAACTAAAGTTGGGGTCGAACAGAATCTTGTGGATTGGGCAAGACCTTACTTGGCCGATAAGCGCAAGTTATTTCGGATAATGGACACCAAGTTAGCGGGCCAATACCCTCAAAAAGCTGCTTACATGACTACAGTCCTTGCATTGCAGTGCATTCGGGAGGCTAAATTCCGACCACAGATGTCCGAAGTTTTATATGCTCTCGAACAGCTCCTCCTGGTTAAGCCTGCCGATAACCAAAGAGCAGACAGTCCTGGGCCGAAGTCCCCACTAAGACATAAGTATTCTTCACCTGTAAATTCCACGACACCGAGAGGGAGCTCTCCGTTACCATCCAAATGAAAACCATAGGTACATGTTGAGAAGGGATGGATCATCTTTATCGCAATGACCCAAGTAAATAAATTTTGACTTCAGCACGTTTGGTTGGTGGATTGTAGTAGTCGAAAATTTCATCACCCCTTTCGTCTTCGGCTATAGGTTGGGCGAAGCAAAGATACAGGTATCGTGACGGAAGAGGATGGTAAATACAGTCATCAACCTCACCAAAGTCTGTATTCTGCATATGAAGAGGAATTGCTGTTGGGTGTGGTTCTTGTAACAACAGCAGAGCAGTGAAAGTAGTTTGGTTTTATAAAATGAAATGGAGCTGAGTTTGTTTGGGAGGTGGGAAAATTTTGCAGAGATAATATTATTACCTTACATAGAAGGAATGGGAATAATGAGTAGCATAGTTGACATTTTACTTTGAGGATTTTAAGATTGACAATCAAAGCTTAAAATATCATGGAAGGTTTCTATTGGCTGTATAATTTCTTATGTTGTAGGCAAGGCCACCAACCAAGGTTTTGGTTTTGGTTTTGGTTTTTGTTTTTTGTTTTGTCTTTTTCGTTGAACAATAACAGAGAGAGAAGCAAAAGAGTTCACAATGATTTTGAGAGATTATGTCATCTATAGTTATTTATTAATTTGTATAAAAAATGGAGATATTTCAT

mRNA sequence

ATGCTGAGACCAGAAGAACCGAAAAATCATGATAGGAAAGGCCATTCATCTGCTTCTTTGAGAGCTCCATCACTTGGTGGGAATACTCCTTCTAGAGGTCTTCAAGCTCATAGATCCGAAGGTGAAATATTGTTGTCTCCAAATCTGAAAGCCTTCACATACAGTGAGCTTAAGAATGCTACTAAAAACTTCCGCCCTGACAGTCTCATTGGAGAAGGAGGTTTTGGTCATGTTTACAAAGGATGGATTGATGAACATACACTTGAAGCTGTGCGGCCTGGAGCTGGAATGGTTGTTGCTGTCAAGAAGCTTAAACCTGAAGGTTTTCAAGGGCACAAAGAATGGCTGTCTGAAGTAAATTACCTCGGTCAGCTTCATCATCCAAATCTTGTTAAACTAATCGGATTCTGCCTTGATGGAGATAGCCGACTTTTGGTCTATGAGTACATGTCCAAAGGCAGCCTTGAAAATCATCTATTTAGAAGGGGCGCACGTCCACTTTCTTGGGCAATAAGGATCAAAGTTGCTATAGGTGCTGCTCGAGGGCTTTCCTTTCTGCATGACTCTGAAGAACCAGTTATCTACCGCGATTTTAAGGCATCAAACATCCTTTTAGATTCTGAATTCAACGCGAAACTTTCAGATTTTGGTTTGGCAAAAGCAGGCCCAACTGGCGATCGGACTCATGTGTCCACTCAAGTCATGGGTACTCAAGGCTATGCTGCTCCAGAATATATTGCCACTGGTAGGCTAACGGCCAAGTGTGACGTTTATAGCTTTGGGGTTGTGCTGCTGGAGCTCCTCTCTGGCCGCCGTGCTGTCGATAAAACTAAAGTTGGGGTCGAACAGAATCTTGTGGATTGGGCAAGACCTTACTTGGCCGATAAGCGCAAGTTATTTCGGATAATGGACACCAAGTTAGCGGGCCAATACCCTCAAAAAGCTGCTTACATGACTACAGTCCTTGCATTGCAGTGCATTCGGGAGGCTAAATTCCGACCACAGATGTCCGAAGTTTTATATGCTCTCGAACAGCTCCTCCTGGTTAAGCCTGCCGATAACCAAAGAGCAGACAGTCCTGGGCCGAAGTCCCCACTAAGACATAAGTATTCTTCACCTGTAAATTCCACGACACCGAGAGGGAGCTCTCCGTTACCATCCAAATGA

Coding sequence (CDS)

ATGCTGAGACCAGAAGAACCGAAAAATCATGATAGGAAAGGCCATTCATCTGCTTCTTTGAGAGCTCCATCACTTGGTGGGAATACTCCTTCTAGAGGTCTTCAAGCTCATAGATCCGAAGGTGAAATATTGTTGTCTCCAAATCTGAAAGCCTTCACATACAGTGAGCTTAAGAATGCTACTAAAAACTTCCGCCCTGACAGTCTCATTGGAGAAGGAGGTTTTGGTCATGTTTACAAAGGATGGATTGATGAACATACACTTGAAGCTGTGCGGCCTGGAGCTGGAATGGTTGTTGCTGTCAAGAAGCTTAAACCTGAAGGTTTTCAAGGGCACAAAGAATGGCTGTCTGAAGTAAATTACCTCGGTCAGCTTCATCATCCAAATCTTGTTAAACTAATCGGATTCTGCCTTGATGGAGATAGCCGACTTTTGGTCTATGAGTACATGTCCAAAGGCAGCCTTGAAAATCATCTATTTAGAAGGGGCGCACGTCCACTTTCTTGGGCAATAAGGATCAAAGTTGCTATAGGTGCTGCTCGAGGGCTTTCCTTTCTGCATGACTCTGAAGAACCAGTTATCTACCGCGATTTTAAGGCATCAAACATCCTTTTAGATTCTGAATTCAACGCGAAACTTTCAGATTTTGGTTTGGCAAAAGCAGGCCCAACTGGCGATCGGACTCATGTGTCCACTCAAGTCATGGGTACTCAAGGCTATGCTGCTCCAGAATATATTGCCACTGGTAGGCTAACGGCCAAGTGTGACGTTTATAGCTTTGGGGTTGTGCTGCTGGAGCTCCTCTCTGGCCGCCGTGCTGTCGATAAAACTAAAGTTGGGGTCGAACAGAATCTTGTGGATTGGGCAAGACCTTACTTGGCCGATAAGCGCAAGTTATTTCGGATAATGGACACCAAGTTAGCGGGCCAATACCCTCAAAAAGCTGCTTACATGACTACAGTCCTTGCATTGCAGTGCATTCGGGAGGCTAAATTCCGACCACAGATGTCCGAAGTTTTATATGCTCTCGAACAGCTCCTCCTGGTTAAGCCTGCCGATAACCAAAGAGCAGACAGTCCTGGGCCGAAGTCCCCACTAAGACATAAGTATTCTTCACCTGTAAATTCCACGACACCGAGAGGGAGCTCTCCGTTACCATCCAAATGA

Protein sequence

MLRPEEPKNHDRKGHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNFRPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQLHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDTKLAGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLLLVKPADNQRADSPGPKSPLRHKYSSPVNSTTPRGSSPLPSK
Homology
BLAST of Spg036901 vs. NCBI nr
Match: XP_038895448.1 (probable serine/threonine-protein kinase PBL3 isoform X1 [Benincasa hispida])

HSP 1 Score: 748.4 bits (1931), Expect = 3.0e-212
Identity = 371/384 (96.61%), Postives = 375/384 (97.66%), Query Frame = 0

Query: 5   EEPKNHDRKGHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNF 64
           EEPKNHDRKGHSSASLRAPSLGGNTPSRGLQ HRSEGEILLSPNLKAFTYSELKNATKNF
Sbjct: 76  EEPKNHDRKGHSSASLRAPSLGGNTPSRGLQVHRSEGEILLSPNLKAFTYSELKNATKNF 135

Query: 65  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 124
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 136 RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 195

Query: 125 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 184
           LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGL+
Sbjct: 196 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLT 255

Query: 185 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 244
           FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 256 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 315

Query: 245 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMD 304
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLV+WARPYLADKRKLFRIMD
Sbjct: 316 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVEWARPYLADKRKLFRIMD 375

Query: 305 TKLAGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLLLVKPADNQRADSPGPKS 364
            KL GQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQL LVK   NQRA+SPGPKS
Sbjct: 376 IKLEGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLPLVKHVHNQRANSPGPKS 435

Query: 365 PLRHKYSSPVNSTTPRGSSPLPSK 389
           PLRHKY SPVNSTTP+ SSPLPSK
Sbjct: 436 PLRHKYPSPVNSTTPKASSPLPSK 459

BLAST of Spg036901 vs. NCBI nr
Match: XP_008437450.1 (PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis melo])

HSP 1 Score: 738.8 bits (1906), Expect = 2.4e-209
Identity = 366/384 (95.31%), Postives = 373/384 (97.14%), Query Frame = 0

Query: 5   EEPKNHDRKGHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNF 64
           E+PK HDRKGHSSASLRAPSLGGNTPSRGLQ HR+EGEILLSPNLKAFTYSELKNATKNF
Sbjct: 23  EDPKAHDRKGHSSASLRAPSLGGNTPSRGLQVHRTEGEILLSPNLKAFTYSELKNATKNF 82

Query: 65  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 124
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 125 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 184
           LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGL+
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLT 202

Query: 185 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 244
           FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 245 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMD 304
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLV+WARPYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVEWARPYLADKRKLFRIMD 322

Query: 305 TKLAGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLLLVKPADNQRADSPGPKS 364
            KL GQYPQKAAYMTTVLALQCIREAKFRPQMSEVL ALEQL LVK  D QRA+SPGPKS
Sbjct: 323 IKLEGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLSALEQLPLVKHVDTQRANSPGPKS 382

Query: 365 PLRHKYSSPVNSTTPRGSSPLPSK 389
           PLRHKY SP+NSTTP+ SSPLPSK
Sbjct: 383 PLRHKYPSPLNSTTPKASSPLPSK 406

BLAST of Spg036901 vs. NCBI nr
Match: XP_011651142.1 (probable serine/threonine-protein kinase PBL3 isoform X1 [Cucumis sativus] >KAE8652573.1 hypothetical protein Csa_013876 [Cucumis sativus])

HSP 1 Score: 738.8 bits (1906), Expect = 2.4e-209
Identity = 365/384 (95.05%), Postives = 373/384 (97.14%), Query Frame = 0

Query: 5   EEPKNHDRKGHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNF 64
           E+PK HDRKGHSSASLR+PSLGGNTPSRGLQ HR+EGEILLSPNLKAFTYSELKNATKNF
Sbjct: 23  EDPKTHDRKGHSSASLRSPSLGGNTPSRGLQVHRTEGEILLSPNLKAFTYSELKNATKNF 82

Query: 65  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 124
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 125 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 184
           LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGL+
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLT 202

Query: 185 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 244
           FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 245 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMD 304
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLV+WARPYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVEWARPYLADKRKLFRIMD 322

Query: 305 TKLAGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLLLVKPADNQRADSPGPKS 364
            KL GQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQL LVK  D QRA+SPGPKS
Sbjct: 323 IKLEGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLPLVKHVDTQRANSPGPKS 382

Query: 365 PLRHKYSSPVNSTTPRGSSPLPSK 389
           PLRHKY SP+NS TP+ SSPLPSK
Sbjct: 383 PLRHKYPSPLNSMTPKPSSPLPSK 406

BLAST of Spg036901 vs. NCBI nr
Match: XP_031745128.1 (probable serine/threonine-protein kinase PBL3 isoform X2 [Cucumis sativus])

HSP 1 Score: 737.3 bits (1902), Expect = 7.0e-209
Identity = 364/383 (95.04%), Postives = 372/383 (97.13%), Query Frame = 0

Query: 6   EPKNHDRKGHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNFR 65
           +PK HDRKGHSSASLR+PSLGGNTPSRGLQ HR+EGEILLSPNLKAFTYSELKNATKNFR
Sbjct: 23  DPKTHDRKGHSSASLRSPSLGGNTPSRGLQVHRTEGEILLSPNLKAFTYSELKNATKNFR 82

Query: 66  PDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQL 125
           PDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQL
Sbjct: 83  PDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQL 142

Query: 126 HHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSF 185
           HHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGL+F
Sbjct: 143 HHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLTF 202

Query: 186 LHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY 245
           LHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY
Sbjct: 203 LHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY 262

Query: 246 IATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDT 305
           IATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLV+WARPYLADKRKLFRIMD 
Sbjct: 263 IATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVEWARPYLADKRKLFRIMDI 322

Query: 306 KLAGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLLLVKPADNQRADSPGPKSP 365
           KL GQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQL LVK  D QRA+SPGPKSP
Sbjct: 323 KLEGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLPLVKHVDTQRANSPGPKSP 382

Query: 366 LRHKYSSPVNSTTPRGSSPLPSK 389
           LRHKY SP+NS TP+ SSPLPSK
Sbjct: 383 LRHKYPSPLNSMTPKPSSPLPSK 405

BLAST of Spg036901 vs. NCBI nr
Match: KAG6584267.1 (putative serine/threonine-protein kinase PBL3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 716.8 bits (1849), Expect = 9.8e-203
Identity = 357/384 (92.97%), Postives = 366/384 (95.31%), Query Frame = 0

Query: 5   EEPKNHDRKGHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNF 64
           EEPK HDRKGHSSASLRAPSLGG+TPSRGLQAHRSEGEILLSPNLKAFTYSEL+NATKNF
Sbjct: 23  EEPKKHDRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 82

Query: 65  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 124
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 125 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 184
           LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 202

Query: 185 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 244
           FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 245 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMD 304
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVG+EQNLV+WA+PYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 322

Query: 305 TKLAGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLLLVKPADNQRADSPGPKS 364
           TKL GQYPQKAAYMTTVLALQCIREAK RPQMSEVL +LE+L LVK  DNQR +SPGPKS
Sbjct: 323 TKLEGQYPQKAAYMTTVLALQCIREAKLRPQMSEVLSSLEELPLVKHVDNQRLNSPGPKS 382

Query: 365 PLRHKYSSPVNSTTPRGSSPLPSK 389
           PLRH         TP+ SSPLPSK
Sbjct: 383 PLRH---------TPKASSPLPSK 397

BLAST of Spg036901 vs. ExPASy Swiss-Prot
Match: O49840 (Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN=PBL3 PE=1 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 3.4e-150
Identity = 266/377 (70.56%), Postives = 313/377 (83.02%), Query Frame = 0

Query: 24  SLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNFRPDSLIGEGGFGHVYKGWI 83
           S+   +    L   R+EGEIL SPNLKAFT++ELKNAT+NFRPDSL+GEGGFG+V+KGWI
Sbjct: 43  SINSYSSVESLPTPRTEGEILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWI 102

Query: 84  DEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQLHHPNLVKLIGFCLDGDSR 143
           D  TL A +PG+G+VVAVKKLK EG+QGHKEWL+EVNYLGQL HPNLVKL+G+C++G++R
Sbjct: 103 DGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSHPNLVKLVGYCVEGENR 162

Query: 144 LLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLHDSEEPVIYRDFKASNI 203
           LLVYE+M KGSLENHLFRRGA+PL+WAIR+KVAIGAA+GL+FLHD++  VIYRDFKA+NI
Sbjct: 163 LLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLHDAKSQVIYRDFKAANI 222

Query: 204 LLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAKCDVYSFGVV 263
           LLD+EFN+KLSDFGLAKAGPTGD+THVSTQVMGT GYAAPEY+ATGRLTAK DVYSFGVV
Sbjct: 223 LLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDVYSFGVV 282

Query: 264 LLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDTKLAGQYPQKAAYMTTVLA 323
           LLELLSGRRAVDK+KVG+EQ+LVDWA PYL DKRKLFRIMDT+L GQYPQK AY    LA
Sbjct: 283 LLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRLGGQYPQKGAYTAASLA 342

Query: 324 LQCIR-EAKFRPQMSEVLYALEQLLLVKP---ADNQRADSPGP---------KSPLRHKY 383
           LQC+  +AK RP+MSEVL  L+QL   KP     N++A    P         KSP R+ Y
Sbjct: 343 LQCLNPDAKLRPKMSEVLAKLDQLESTKPGTGVGNRQAQIDSPRGSNGSIVQKSPRRYSY 402

Query: 384 SSPVNSTTPRGSSPLPS 388
             P+   TP G+SPLP+
Sbjct: 403 DRPLLHITP-GASPLPT 418

BLAST of Spg036901 vs. ExPASy Swiss-Prot
Match: O49839 (Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN=PBL2 PE=1 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 1.5e-142
Identity = 257/384 (66.93%), Postives = 306/384 (79.69%), Query Frame = 0

Query: 14  GHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNFRPDSLIGEG 73
           G S+ S    S  G  P+      R+EGEIL SPNLKAFT++ELKNATKNFR D+L+GEG
Sbjct: 41  GLSTTSYSTDSSFGPLPTL-----RTEGEILSSPNLKAFTFNELKNATKNFRQDNLLGEG 100

Query: 74  GFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQLHHPNLVKL 133
           GFG V+KGWID+ +L A RPG+G+VVAVK+LKPEGFQGHKEWL+EVNYLGQL HPNLV L
Sbjct: 101 GFGCVFKGWIDQTSLTASRPGSGIVVAVKQLKPEGFQGHKEWLTEVNYLGQLSHPNLVLL 160

Query: 134 IGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLHDSEEPV 193
           +G+C +G++RLLVYE+M KGSLENHLFRRGA+PL+WAIR+KVA+GAA+GL+FLH+++  V
Sbjct: 161 VGYCAEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAVGAAKGLTFLHEAKSQV 220

Query: 194 IYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTA 253
           IYRDFKA+NILLD++FNAKLSDFGLAKAGPTGD THVST+V+GT GYAAPEY+ATGRLTA
Sbjct: 221 IYRDFKAANILLDADFNAKLSDFGLAKAGPTGDNTHVSTKVIGTHGYAAPEYVATGRLTA 280

Query: 254 KCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDTKLAGQYPQ 313
           K DVYSFGVVLLEL+SGRRA+D +  G E +LVDWA PYL DKRKLFRIMDTKL GQYPQ
Sbjct: 281 KSDVYSFGVVLLELISGRRAMDNSNGGNEYSLVDWATPYLGDKRKLFRIMDTKLGGQYPQ 340

Query: 314 KAAYMTTVLALQCIR-EAKFRPQMSEVLYALEQLLLVKPADNQRADSPGP---------K 373
           K A+    LALQC+  +AK RP+MSEVL  LEQL  V     +      P         K
Sbjct: 341 KGAFTAANLALQCLNPDAKLRPKMSEVLVTLEQLESVAKPGTKHTQMESPRFHHSSVMQK 400

Query: 374 SPLRHKYSSPVNSTTPRGSSPLPS 388
           SP+R+ +  P+   TP G+SPLPS
Sbjct: 401 SPVRYSHDRPLLHMTP-GASPLPS 418

BLAST of Spg036901 vs. ExPASy Swiss-Prot
Match: Q5PP29 (Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN=PBL4 PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.4e-140
Identity = 249/333 (74.77%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 16  SSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNFRPDSLIGEGGF 75
           SS ++++ S   +T    LQ  RSEGE+L SP LKAFT++ELK AT+NFRPDS+IGEGGF
Sbjct: 35  SSLTIQSSSYNDDTSVASLQTPRSEGELLASPTLKAFTFNELKTATRNFRPDSVIGEGGF 94

Query: 76  GHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQLHHPNLVKLIG 135
           G+VYKGWIDE TL   +PG+GMVVAVKKLK EGFQGH++WL+EV+ LG+LHH NLVKLIG
Sbjct: 95  GYVYKGWIDERTLSPSKPGSGMVVAVKKLKEEGFQGHRQWLAEVDCLGRLHHMNLVKLIG 154

Query: 136 FCLDGDS-RLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLHDSEEPVI 195
           +C  GD  RLLVYEYM KGSLENHLFRRGA P+ W  RIKVAIGAARGL+FLH+++  VI
Sbjct: 155 YCSKGDHIRLLVYEYMPKGSLENHLFRRGAEPIPWRTRIKVAIGAARGLAFLHEAQ--VI 214

Query: 196 YRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAK 255
           YRDFKASNILLDSEFNAKLSDFGLAK GPTGDRTHVSTQVMGTQGYAAPEY+ATGR+TAK
Sbjct: 215 YRDFKASNILLDSEFNAKLSDFGLAKVGPTGDRTHVSTQVMGTQGYAAPEYVATGRITAK 274

Query: 256 CDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDTKLAGQYPQK 315
            DVYSFGVVLLELLSGR  VDKTKVGVE+NLVDWA PYL DKRK+FRIMDTKL GQYP K
Sbjct: 275 SDVYSFGVVLLELLSGRLTVDKTKVGVERNLVDWAIPYLGDKRKVFRIMDTKLGGQYPHK 334

Query: 316 AAYMTTVLALQCI-REAKFRPQMSEVLYALEQL 347
            A +T   ALQC+ +E K RP+MS+VL  LE+L
Sbjct: 335 GACLTANTALQCLNQEPKLRPKMSDVLSTLEEL 365

BLAST of Spg036901 vs. ExPASy Swiss-Prot
Match: Q5XF79 (Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 GN=PBL18 PE=2 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 2.6e-134
Identity = 239/333 (71.77%), Postives = 276/333 (82.88%), Query Frame = 0

Query: 18  ASLRAPSLGGN---TPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNFRPDSLIGEGG 77
           +SL  PS   N   T S      RSEGE+L SP LKAFT++ELK AT+NF+P+S+IGEGG
Sbjct: 35  SSLTIPSYSNNSFTTSSWSNLTPRSEGELLPSPTLKAFTFNELKTATRNFKPNSMIGEGG 94

Query: 78  FGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQLHHPNLVKLI 137
           FG VYKGWI E +L   +PG+GMVVAVKKLK EGFQGHKEWL+EV+YLG+LHH NLVKLI
Sbjct: 95  FGCVYKGWIGERSLSPSKPGSGMVVAVKKLKSEGFQGHKEWLTEVHYLGRLHHMNLVKLI 154

Query: 138 GFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLHDSEEPVI 197
           G+CL+G+ RLLVYEYM KGSLENHLFRRGA P+ W  R+KVA  AARGLSFLH+++  VI
Sbjct: 155 GYCLEGEKRLLVYEYMPKGSLENHLFRRGAEPIPWKTRMKVAFSAARGLSFLHEAK--VI 214

Query: 198 YRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAK 257
           YRDFKASNILLD +FNAKLSDFGLAKAGPTGDRTHV+TQV+GTQGYAAPEYIATGRLT+K
Sbjct: 215 YRDFKASNILLDVDFNAKLSDFGLAKAGPTGDRTHVTTQVIGTQGYAAPEYIATGRLTSK 274

Query: 258 CDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDTKLAGQYPQK 317
            DVYSFGVVLLELLSGR  +DK+KVGVE+NLVDWA PYL D+RK+FRIMDTKL GQYP K
Sbjct: 275 SDVYSFGVVLLELLSGRPTLDKSKVGVERNLVDWAIPYLVDRRKVFRIMDTKLGGQYPHK 334

Query: 318 AAYMTTVLALQCIR-EAKFRPQMSEVLYALEQL 347
            A     +AL+C+  E K RP M++VL  L+QL
Sbjct: 335 GACAAANIALRCLNTEPKLRPDMADVLSTLQQL 365

BLAST of Spg036901 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 453.0 bits (1164), Expect = 3.4e-126
Identity = 219/339 (64.60%), Postives = 274/339 (80.83%), Query Frame = 0

Query: 24  SLGGNTPSRGLQAH-RSEGEILLSPNLKAFTYSELKNATKNFRPDSLIGEGGFGHVYKGW 83
           SLG  + S  ++ + R+EGEIL SPNLK+FT++ELK AT+NFRPDS++GEGGFG V+KGW
Sbjct: 28  SLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGW 87

Query: 84  IDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQLHHPNLVKLIGFCLDGDS 143
           IDE TL A +PG G+V+AVKKL  +G+QGH+EWL+EVNYLGQ  HPNLVKLIG+CL+ + 
Sbjct: 88  IDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEH 147

Query: 144 RLLVYEYMSKGSLENHLFRRGA--RPLSWAIRIKVAIGAARGLSFLHDSEEPVIYRDFKA 203
           RLLVYE+M +GSLENHLFRRG+  +PLSW +R+KVA+GAA+GL+FLH++E  VIYRDFK 
Sbjct: 148 RLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFKT 207

Query: 204 SNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAKCDVYSF 263
           SNILLDSE+NAKLSDFGLAK GPTGD++HVST++MGT GYAAPEY+ATG LT K DVYS+
Sbjct: 208 SNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSY 267

Query: 264 GVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDTKLAGQYPQKAAYMTT 323
           GVVLLE+LSGRRAVDK +   EQ LV+WARP LA+KRKLFR++D +L  QY  + A    
Sbjct: 268 GVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVA 327

Query: 324 VLALQCIR-EAKFRPQMSEVLYALEQLLLVKPADNQRAD 359
            LAL+C+  E K RP M+EV+  LE +  +  A  +  D
Sbjct: 328 TLALRCLTFEIKLRPNMNEVVSHLEHIQTLNEAGGRNID 366

BLAST of Spg036901 vs. ExPASy TrEMBL
Match: A0A1S3AU61 (protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103482862 PE=3 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.2e-209
Identity = 366/384 (95.31%), Postives = 373/384 (97.14%), Query Frame = 0

Query: 5   EEPKNHDRKGHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNF 64
           E+PK HDRKGHSSASLRAPSLGGNTPSRGLQ HR+EGEILLSPNLKAFTYSELKNATKNF
Sbjct: 23  EDPKAHDRKGHSSASLRAPSLGGNTPSRGLQVHRTEGEILLSPNLKAFTYSELKNATKNF 82

Query: 65  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 124
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 125 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 184
           LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGL+
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLT 202

Query: 185 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 244
           FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 245 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMD 304
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLV+WARPYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVEWARPYLADKRKLFRIMD 322

Query: 305 TKLAGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLLLVKPADNQRADSPGPKS 364
            KL GQYPQKAAYMTTVLALQCIREAKFRPQMSEVL ALEQL LVK  D QRA+SPGPKS
Sbjct: 323 IKLEGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLSALEQLPLVKHVDTQRANSPGPKS 382

Query: 365 PLRHKYSSPVNSTTPRGSSPLPSK 389
           PLRHKY SP+NSTTP+ SSPLPSK
Sbjct: 383 PLRHKYPSPLNSTTPKASSPLPSK 406

BLAST of Spg036901 vs. ExPASy TrEMBL
Match: A0A6J1KLE7 (probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495635 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 8.1e-203
Identity = 357/384 (92.97%), Postives = 366/384 (95.31%), Query Frame = 0

Query: 5   EEPKNHDRKGHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNF 64
           EEPK H+RKGHSSASLRAPSLGG+TPSRGLQAHRSEGEILLSPNLKAFTYSEL+NATKNF
Sbjct: 23  EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 82

Query: 65  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 124
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 125 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 184
           LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 202

Query: 185 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 244
           FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 245 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMD 304
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVG+EQNLV+WARPYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWARPYLADKRKLFRIMD 322

Query: 305 TKLAGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLLLVKPADNQRADSPGPKS 364
           TKL GQYPQKAAYMTTVLALQCIREAK RPQMSEVL +LE+L LVK  DNQR +SPGPKS
Sbjct: 323 TKLEGQYPQKAAYMTTVLALQCIREAKLRPQMSEVLSSLEELPLVKHVDNQRLNSPGPKS 382

Query: 365 PLRHKYSSPVNSTTPRGSSPLPSK 389
           PLRH         TP+ SSPLPSK
Sbjct: 383 PLRH---------TPKASSPLPSK 397

BLAST of Spg036901 vs. ExPASy TrEMBL
Match: A0A6J1E7M0 (probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431470 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 1.8e-202
Identity = 356/384 (92.71%), Postives = 366/384 (95.31%), Query Frame = 0

Query: 5   EEPKNHDRKGHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNF 64
           EEPK H+RKGHSSASLRAPSLGG+TPSRGLQAHRSEGEILLSPNLKAFTYSEL+NATKNF
Sbjct: 23  EEPKKHNRKGHSSASLRAPSLGGSTPSRGLQAHRSEGEILLSPNLKAFTYSELRNATKNF 82

Query: 65  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 124
           RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ
Sbjct: 83  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 142

Query: 125 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 184
           LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS
Sbjct: 143 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 202

Query: 185 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 244
           FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE
Sbjct: 203 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 262

Query: 245 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMD 304
           YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVG+EQNLV+WA+PYLADKRKLFRIMD
Sbjct: 263 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGIEQNLVEWAKPYLADKRKLFRIMD 322

Query: 305 TKLAGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLLLVKPADNQRADSPGPKS 364
           TKL GQYPQKAAYMTTVLALQCIREAK RPQMSEVL +LE+L LVK  DNQR +SPGPKS
Sbjct: 323 TKLEGQYPQKAAYMTTVLALQCIREAKLRPQMSEVLSSLEELPLVKHVDNQRLNSPGPKS 382

Query: 365 PLRHKYSSPVNSTTPRGSSPLPSK 389
           PLRH         TP+ SSPLPSK
Sbjct: 383 PLRH---------TPKASSPLPSK 397

BLAST of Spg036901 vs. ExPASy TrEMBL
Match: A0A6J1C6M0 (probable serine/threonine-protein kinase PBL3 OS=Momordica charantia OX=3673 GN=LOC111008885 PE=3 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 7.8e-198
Identity = 352/387 (90.96%), Postives = 365/387 (94.32%), Query Frame = 0

Query: 6   EPKNHDRKGHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNFR 65
           EPK  + K HSSA+L+APSLG N PSR LQAHRSEGEILLSPNLKAFT+SELKNATKNFR
Sbjct: 22  EPKKSEMKDHSSATLKAPSLGVNVPSRSLQAHRSEGEILLSPNLKAFTFSELKNATKNFR 81

Query: 66  PDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQL 125
           PDSLIGEGGFG+VYKGWIDEHTLEAVRPGAGMV+AVKKLKPEGFQGHKEWLSEVNYLGQL
Sbjct: 82  PDSLIGEGGFGYVYKGWIDEHTLEAVRPGAGMVIAVKKLKPEGFQGHKEWLSEVNYLGQL 141

Query: 126 HHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSF 185
           HH NLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSF
Sbjct: 142 HHSNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSF 201

Query: 186 LHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY 245
           LH+SEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY
Sbjct: 202 LHNSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEY 261

Query: 246 IATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDT 305
           IATGRLTA+CDVYSFGVVLLELLSGRRAVDKTKVGVEQ+LVDWA+PYLADKRKLFRIMDT
Sbjct: 262 IATGRLTARCDVYSFGVVLLELLSGRRAVDKTKVGVEQHLVDWAKPYLADKRKLFRIMDT 321

Query: 306 KLAGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLLLVKPADNQ----RADSPG 365
           KL GQYPQ+AAYMTT+LALQCIREAKFRPQMSEVL ALEQL LVK ADNQ    RA SPG
Sbjct: 322 KLEGQYPQRAAYMTTILALQCIREAKFRPQMSEVLSALEQLPLVKHADNQRERERAPSPG 381

Query: 366 PKSPLRHKYSSPVNSTTPR-GSSPLPS 388
           PKSPLRHKY SPVNST  + G SPLPS
Sbjct: 382 PKSPLRHKYPSPVNSTASKGGGSPLPS 408

BLAST of Spg036901 vs. ExPASy TrEMBL
Match: A0A6J1GSG8 (probable serine/threonine-protein kinase PBL3 OS=Cucurbita moschata OX=3662 GN=LOC111457081 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.2e-194
Identity = 338/383 (88.25%), Postives = 359/383 (93.73%), Query Frame = 0

Query: 5   EEPKNHDRKGHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNF 64
           E P N DRKGHS+A+LRAP+LGGNTPSRGLQ  R+EGEILLSPNLKAFTYSELKNATK+F
Sbjct: 22  ETPNNQDRKGHSTAALRAPALGGNTPSRGLQPKRTEGEILLSPNLKAFTYSELKNATKSF 81

Query: 65  RPDSLIGEGGFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQ 124
           RPD+LIGEGGFGHVYKGW+DE TLE+V+PG GMVVAVKKLK EGFQGHKEWLSEV +L Q
Sbjct: 82  RPDTLIGEGGFGHVYKGWLDEQTLESVKPGTGMVVAVKKLKSEGFQGHKEWLSEVTHLSQ 141

Query: 125 LHHPNLVKLIGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLS 184
           LHHP+LVKLIGFCLDG+SRLLVYEYMSKGSLENHLFR GARPLSWA RIKVAIGAARGLS
Sbjct: 142 LHHPHLVKLIGFCLDGESRLLVYEYMSKGSLENHLFRMGARPLSWARRIKVAIGAARGLS 201

Query: 185 FLHDSEEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPE 244
           FLH+S EPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDR+HVST+V+GTQGYAAPE
Sbjct: 202 FLHNSAEPVIYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRSHVSTRVIGTQGYAAPE 261

Query: 245 YIATGRLTAKCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMD 304
           YIATG LTAKCDVYSFGVVLLELLSGRR VDKTKVGVEQ+LVDWARPYLADKRKLFRIMD
Sbjct: 262 YIATGMLTAKCDVYSFGVVLLELLSGRRVVDKTKVGVEQHLVDWARPYLADKRKLFRIMD 321

Query: 305 TKLAGQYPQKAAYMTTVLALQCIREAKFRPQMSEVLYALEQLLLVKPADNQRADSPGPKS 364
           T+L G+YPQKAAYMTTVLALQCIREAKFRP MSEVLYALEQLLLVKP DNQRA SPGPKS
Sbjct: 322 TRLEGKYPQKAAYMTTVLALQCIREAKFRPLMSEVLYALEQLLLVKPTDNQRASSPGPKS 381

Query: 365 PLRHKYSSPVNSTTPRGSSPLPS 388
           P+ H   SPVNSTT +GSSPLPS
Sbjct: 382 PITHNLPSPVNSTTQKGSSPLPS 404

BLAST of Spg036901 vs. TAIR 10
Match: AT2G02800.1 (protein kinase 2B )

HSP 1 Score: 532.7 bits (1371), Expect = 2.4e-151
Identity = 266/377 (70.56%), Postives = 313/377 (83.02%), Query Frame = 0

Query: 24  SLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNFRPDSLIGEGGFGHVYKGWI 83
           S+   +    L   R+EGEIL SPNLKAFT++ELKNAT+NFRPDSL+GEGGFG+V+KGWI
Sbjct: 43  SINSYSSVESLPTPRTEGEILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWI 102

Query: 84  DEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQLHHPNLVKLIGFCLDGDSR 143
           D  TL A +PG+G+VVAVKKLK EG+QGHKEWL+EVNYLGQL HPNLVKL+G+C++G++R
Sbjct: 103 DGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSHPNLVKLVGYCVEGENR 162

Query: 144 LLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLHDSEEPVIYRDFKASNI 203
           LLVYE+M KGSLENHLFRRGA+PL+WAIR+KVAIGAA+GL+FLHD++  VIYRDFKA+NI
Sbjct: 163 LLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLHDAKSQVIYRDFKAANI 222

Query: 204 LLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAKCDVYSFGVV 263
           LLD+EFN+KLSDFGLAKAGPTGD+THVSTQVMGT GYAAPEY+ATGRLTAK DVYSFGVV
Sbjct: 223 LLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDVYSFGVV 282

Query: 264 LLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDTKLAGQYPQKAAYMTTVLA 323
           LLELLSGRRAVDK+KVG+EQ+LVDWA PYL DKRKLFRIMDT+L GQYPQK AY    LA
Sbjct: 283 LLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRLGGQYPQKGAYTAASLA 342

Query: 324 LQCIR-EAKFRPQMSEVLYALEQLLLVKP---ADNQRADSPGP---------KSPLRHKY 383
           LQC+  +AK RP+MSEVL  L+QL   KP     N++A    P         KSP R+ Y
Sbjct: 343 LQCLNPDAKLRPKMSEVLAKLDQLESTKPGTGVGNRQAQIDSPRGSNGSIVQKSPRRYSY 402

Query: 384 SSPVNSTTPRGSSPLPS 388
             P+   TP G+SPLP+
Sbjct: 403 DRPLLHITP-GASPLPT 418

BLAST of Spg036901 vs. TAIR 10
Match: AT2G02800.2 (protein kinase 2B )

HSP 1 Score: 532.7 bits (1371), Expect = 2.4e-151
Identity = 266/377 (70.56%), Postives = 313/377 (83.02%), Query Frame = 0

Query: 24  SLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNFRPDSLIGEGGFGHVYKGWI 83
           S+   +    L   R+EGEIL SPNLKAFT++ELKNAT+NFRPDSL+GEGGFG+V+KGWI
Sbjct: 43  SINSYSSVESLPTPRTEGEILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWI 102

Query: 84  DEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQLHHPNLVKLIGFCLDGDSR 143
           D  TL A +PG+G+VVAVKKLK EG+QGHKEWL+EVNYLGQL HPNLVKL+G+C++G++R
Sbjct: 103 DGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSHPNLVKLVGYCVEGENR 162

Query: 144 LLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLHDSEEPVIYRDFKASNI 203
           LLVYE+M KGSLENHLFRRGA+PL+WAIR+KVAIGAA+GL+FLHD++  VIYRDFKA+NI
Sbjct: 163 LLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLHDAKSQVIYRDFKAANI 222

Query: 204 LLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAKCDVYSFGVV 263
           LLD+EFN+KLSDFGLAKAGPTGD+THVSTQVMGT GYAAPEY+ATGRLTAK DVYSFGVV
Sbjct: 223 LLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDVYSFGVV 282

Query: 264 LLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDTKLAGQYPQKAAYMTTVLA 323
           LLELLSGRRAVDK+KVG+EQ+LVDWA PYL DKRKLFRIMDT+L GQYPQK AY    LA
Sbjct: 283 LLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRLGGQYPQKGAYTAASLA 342

Query: 324 LQCIR-EAKFRPQMSEVLYALEQLLLVKP---ADNQRADSPGP---------KSPLRHKY 383
           LQC+  +AK RP+MSEVL  L+QL   KP     N++A    P         KSP R+ Y
Sbjct: 343 LQCLNPDAKLRPKMSEVLAKLDQLESTKPGTGVGNRQAQIDSPRGSNGSIVQKSPRRYSY 402

Query: 384 SSPVNSTTPRGSSPLPS 388
             P+   TP G+SPLP+
Sbjct: 403 DRPLLHITP-GASPLPT 418

BLAST of Spg036901 vs. TAIR 10
Match: AT1G14370.1 (protein kinase 2A )

HSP 1 Score: 507.3 bits (1305), Expect = 1.1e-143
Identity = 257/384 (66.93%), Postives = 306/384 (79.69%), Query Frame = 0

Query: 14  GHSSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNFRPDSLIGEG 73
           G S+ S    S  G  P+      R+EGEIL SPNLKAFT++ELKNATKNFR D+L+GEG
Sbjct: 41  GLSTTSYSTDSSFGPLPTL-----RTEGEILSSPNLKAFTFNELKNATKNFRQDNLLGEG 100

Query: 74  GFGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQLHHPNLVKL 133
           GFG V+KGWID+ +L A RPG+G+VVAVK+LKPEGFQGHKEWL+EVNYLGQL HPNLV L
Sbjct: 101 GFGCVFKGWIDQTSLTASRPGSGIVVAVKQLKPEGFQGHKEWLTEVNYLGQLSHPNLVLL 160

Query: 134 IGFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLHDSEEPV 193
           +G+C +G++RLLVYE+M KGSLENHLFRRGA+PL+WAIR+KVA+GAA+GL+FLH+++  V
Sbjct: 161 VGYCAEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAVGAAKGLTFLHEAKSQV 220

Query: 194 IYRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTA 253
           IYRDFKA+NILLD++FNAKLSDFGLAKAGPTGD THVST+V+GT GYAAPEY+ATGRLTA
Sbjct: 221 IYRDFKAANILLDADFNAKLSDFGLAKAGPTGDNTHVSTKVIGTHGYAAPEYVATGRLTA 280

Query: 254 KCDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDTKLAGQYPQ 313
           K DVYSFGVVLLEL+SGRRA+D +  G E +LVDWA PYL DKRKLFRIMDTKL GQYPQ
Sbjct: 281 KSDVYSFGVVLLELISGRRAMDNSNGGNEYSLVDWATPYLGDKRKLFRIMDTKLGGQYPQ 340

Query: 314 KAAYMTTVLALQCIR-EAKFRPQMSEVLYALEQLLLVKPADNQRADSPGP---------K 373
           K A+    LALQC+  +AK RP+MSEVL  LEQL  V     +      P         K
Sbjct: 341 KGAFTAANLALQCLNPDAKLRPKMSEVLVTLEQLESVAKPGTKHTQMESPRFHHSSVMQK 400

Query: 374 SPLRHKYSSPVNSTTPRGSSPLPS 388
           SP+R+ +  P+   TP G+SPLPS
Sbjct: 401 SPVRYSHDRPLLHMTP-GASPLPS 418

BLAST of Spg036901 vs. TAIR 10
Match: AT1G26970.1 (Protein kinase superfamily protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.0e-141
Identity = 249/333 (74.77%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 16  SSASLRAPSLGGNTPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNFRPDSLIGEGGF 75
           SS ++++ S   +T    LQ  RSEGE+L SP LKAFT++ELK AT+NFRPDS+IGEGGF
Sbjct: 35  SSLTIQSSSYNDDTSVASLQTPRSEGELLASPTLKAFTFNELKTATRNFRPDSVIGEGGF 94

Query: 76  GHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQLHHPNLVKLIG 135
           G+VYKGWIDE TL   +PG+GMVVAVKKLK EGFQGH++WL+EV+ LG+LHH NLVKLIG
Sbjct: 95  GYVYKGWIDERTLSPSKPGSGMVVAVKKLKEEGFQGHRQWLAEVDCLGRLHHMNLVKLIG 154

Query: 136 FCLDGDS-RLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLHDSEEPVI 195
           +C  GD  RLLVYEYM KGSLENHLFRRGA P+ W  RIKVAIGAARGL+FLH+++  VI
Sbjct: 155 YCSKGDHIRLLVYEYMPKGSLENHLFRRGAEPIPWRTRIKVAIGAARGLAFLHEAQ--VI 214

Query: 196 YRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAK 255
           YRDFKASNILLDSEFNAKLSDFGLAK GPTGDRTHVSTQVMGTQGYAAPEY+ATGR+TAK
Sbjct: 215 YRDFKASNILLDSEFNAKLSDFGLAKVGPTGDRTHVSTQVMGTQGYAAPEYVATGRITAK 274

Query: 256 CDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDTKLAGQYPQK 315
            DVYSFGVVLLELLSGR  VDKTKVGVE+NLVDWA PYL DKRK+FRIMDTKL GQYP K
Sbjct: 275 SDVYSFGVVLLELLSGRLTVDKTKVGVERNLVDWAIPYLGDKRKVFRIMDTKLGGQYPHK 334

Query: 316 AAYMTTVLALQCI-REAKFRPQMSEVLYALEQL 347
            A +T   ALQC+ +E K RP+MS+VL  LE+L
Sbjct: 335 GACLTANTALQCLNQEPKLRPKMSDVLSTLEEL 365

BLAST of Spg036901 vs. TAIR 10
Match: AT1G69790.1 (Protein kinase superfamily protein )

HSP 1 Score: 479.9 bits (1234), Expect = 1.9e-135
Identity = 239/333 (71.77%), Postives = 276/333 (82.88%), Query Frame = 0

Query: 18  ASLRAPSLGGN---TPSRGLQAHRSEGEILLSPNLKAFTYSELKNATKNFRPDSLIGEGG 77
           +SL  PS   N   T S      RSEGE+L SP LKAFT++ELK AT+NF+P+S+IGEGG
Sbjct: 35  SSLTIPSYSNNSFTTSSWSNLTPRSEGELLPSPTLKAFTFNELKTATRNFKPNSMIGEGG 94

Query: 78  FGHVYKGWIDEHTLEAVRPGAGMVVAVKKLKPEGFQGHKEWLSEVNYLGQLHHPNLVKLI 137
           FG VYKGWI E +L   +PG+GMVVAVKKLK EGFQGHKEWL+EV+YLG+LHH NLVKLI
Sbjct: 95  FGCVYKGWIGERSLSPSKPGSGMVVAVKKLKSEGFQGHKEWLTEVHYLGRLHHMNLVKLI 154

Query: 138 GFCLDGDSRLLVYEYMSKGSLENHLFRRGARPLSWAIRIKVAIGAARGLSFLHDSEEPVI 197
           G+CL+G+ RLLVYEYM KGSLENHLFRRGA P+ W  R+KVA  AARGLSFLH+++  VI
Sbjct: 155 GYCLEGEKRLLVYEYMPKGSLENHLFRRGAEPIPWKTRMKVAFSAARGLSFLHEAK--VI 214

Query: 198 YRDFKASNILLDSEFNAKLSDFGLAKAGPTGDRTHVSTQVMGTQGYAAPEYIATGRLTAK 257
           YRDFKASNILLD +FNAKLSDFGLAKAGPTGDRTHV+TQV+GTQGYAAPEYIATGRLT+K
Sbjct: 215 YRDFKASNILLDVDFNAKLSDFGLAKAGPTGDRTHVTTQVIGTQGYAAPEYIATGRLTSK 274

Query: 258 CDVYSFGVVLLELLSGRRAVDKTKVGVEQNLVDWARPYLADKRKLFRIMDTKLAGQYPQK 317
            DVYSFGVVLLELLSGR  +DK+KVGVE+NLVDWA PYL D+RK+FRIMDTKL GQYP K
Sbjct: 275 SDVYSFGVVLLELLSGRPTLDKSKVGVERNLVDWAIPYLVDRRKVFRIMDTKLGGQYPHK 334

Query: 318 AAYMTTVLALQCIR-EAKFRPQMSEVLYALEQL 347
            A     +AL+C+  E K RP M++VL  L+QL
Sbjct: 335 GACAAANIALRCLNTEPKLRPDMADVLSTLQQL 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895448.13.0e-21296.61probable serine/threonine-protein kinase PBL3 isoform X1 [Benincasa hispida][more]
XP_008437450.12.4e-20995.31PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis melo][more]
XP_011651142.12.4e-20995.05probable serine/threonine-protein kinase PBL3 isoform X1 [Cucumis sativus] >KAE8... [more]
XP_031745128.17.0e-20995.04probable serine/threonine-protein kinase PBL3 isoform X2 [Cucumis sativus][more]
KAG6584267.19.8e-20392.97putative serine/threonine-protein kinase PBL3, partial [Cucurbita argyrosperma s... [more]
Match NameE-valueIdentityDescription
O498403.4e-15070.56Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN... [more]
O498391.5e-14266.93Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q5PP291.4e-14074.77Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q5XF792.6e-13471.77Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 G... [more]
P465733.4e-12664.60Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A1S3AU611.2e-20995.31protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103482862 PE... [more]
A0A6J1KLE78.1e-20392.97probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita maxima OX=... [more]
A0A6J1E7M01.8e-20292.71probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita moschata O... [more]
A0A6J1C6M07.8e-19890.96probable serine/threonine-protein kinase PBL3 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1GSG85.2e-19488.25probable serine/threonine-protein kinase PBL3 OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
AT2G02800.12.4e-15170.56protein kinase 2B [more]
AT2G02800.22.4e-15170.56protein kinase 2B [more]
AT1G14370.11.1e-14366.93protein kinase 2A [more]
AT1G26970.11.0e-14174.77Protein kinase superfamily protein [more]
AT1G69790.11.9e-13571.77Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 68..339
e-value: 3.8E-45
score: 154.2
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 150..359
e-value: 1.3E-55
score: 190.0
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 35..149
e-value: 3.6E-34
score: 119.0
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 70..268
e-value: 7.2E-18
score: 62.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 352..388
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 369..388
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 18..377
NoneNo IPR availablePANTHERPTHR45621:SF72BNAC02G17010D PROTEINcoord: 18..377
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 70..346
e-value: 1.55679E-95
score: 284.55
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 193..205
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 70..103
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 64..347
score: 37.106709
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 43..344

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg036901.1Spg036901.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0015031 protein transport
biological_process GO:0042147 retrograde transport, endosome to Golgi
cellular_component GO:0005829 cytosol
cellular_component GO:0030904 retromer complex
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity