Spg036769 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg036769
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	scaffold5: 45969047 .. 45970559 (-)
RNA-Seq Expression	Spg036769
Synteny	Spg036769

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGCCTTCGTCTTCTTCCTCTGTTTAATTGTCCACTTCGATCCACGAAAAAAAAAAAAGAATGACTTCCAATGGCGTTTCTGCATCAATCTCCAAAAGGTACACAAAAACTCTCCACCCTTCAAATTTCACATCGAAATTCAATTAAAATTCAACTCATCAATTTCTTCATCTCTGTTTCTTTTCTGTTGCAGATTGGCCGGAAAAGTGGCTCTGATAACCGGCGGAGCAAGTGGAATCGGCGAGTCGACGGTGAGGCTGTTTGTCGAGAACGGAGCGAAAGTGGTGATCGCCGACGTCCAGGACGACCTCGCCGCCGCCCTCTGCAAGGAACTCAGCGACGCCGGCGGCGACATTTCCTACTTCCACTGCAATGTCACAGACGAATCTGACATCTCGAACGCGGTCGACTTTGCAGTCGACAAGTATGGAAAGCTCGACATCATGTTCAACAATGCTGGCATTCGCGGCGATGTTACCGCCACGACGTTGACCACGGACATGAACGATTTCAAGAACGTCTTCGACGTTAACGTGTTCGGTACTTTTATGGGAGCCAAACACGCCGCCAGGTATTGAACTATCGCTCCATTCAAAAGCTTAAGTTGTTACGTTAAATATATGTTTCAATTTAGCTTTAAACTAACCTTTTCAATTTTGCAAATAAATTAACTAATTAAATTTACTTGACATGCGTCAAATTTTACAAATTTGAAGGAAGAATTATAAATCTTTTTCCAACGGTATCCAAAGATTAGGATACCAGGCCAGGCCTCCTATAATATCTGTTTATTTAAGTTAATGGGTTACAGTAAATTTAATCATTTATTTATATTCTTGACACCAGGGTCATGGTTCCGGCAAAAACAGGATGCATCCTGTTCACATCGAGCATGGCATCGGTGATATGCAGCGGTAACACACCGGCATATTCGGCGTCGAAGCACGCGATCGTGGGACTGATGAAGACGCTAGCAGTGGAGTTGGGGCCGCACGGGATCAGAGTGAATGCGATATCGCCGTTCGCGACGGTGACGCCGATGCTGCTGCCGTCGAAGGACGTGGAACAGAAGAAGGCAATGGAGGCGTTCATTTCTCTGTCAGGGAACTTGAAAGGGGCAGTAATGGAGGCTGAAGATGTAGCCAAAGCAGCTGTGTACTTAGCGAGTGATGAGTCCAAATATGTGAGCGGATTGAATCTGGTGGTTGATGGTGGCTTCAGTCTTACCAATCCCAGCTTTGAATTTAATGCCAAAGCTTATGGACAGTGAAATATTCGATTGTATGATCAAAAAGTTAGAGATTCGAAAATTTTCATCCTATGTTACAATAACTAGTTTTTGAAAAAAAAAAATAAATAAATGTTGGTTTGTTTTGAAAAATCGAAAGGTTTAAATTAATATGTAATTCAATTAACTAATGACCAGAAGACCTAAAGTCTGTGGCTCGAATCCTCCACTTTATATATTGTCAAAAAGAAAAAAGGGTTAAATTAATATTTCCAATTCTTAGAT

mRNA sequence

ATGACTTCCAATGGCGTTTCTGCATCAATCTCCAAAAGATTGGCCGGAAAAGTGGCTCTGATAACCGGCGGAGCAAGTGGAATCGGCGAGTCGACGGTGAGGCTGTTTGTCGAGAACGGAGCGAAAGTGGTGATCGCCGACGTCCAGGACGACCTCGCCGCCGCCCTCTGCAAGGAACTCAGCGACGCCGGCGGCGACATTTCCTACTTCCACTGCAATGTCACAGACGAATCTGACATCTCGAACGCGGTCGACTTTGCAGTCGACAAGTATGGAAAGCTCGACATCATGTTCAACAATGCTGGCATTCGCGGCGATGTTACCGCCACGACGTTGACCACGGACATGAACGATTTCAAGAACGTCTTCGACGTTAACGTGTTCGGTACTTTTATGGGAGCCAAACACGCCGCCAGGGTCATGGTTCCGGCAAAAACAGGATGCATCCTGTTCACATCGAGCATGGCATCGGTGATATGCAGCGGTAACACACCGGCATATTCGGCGTCGAAGCACGCGATCGTGGGACTGATGAAGACGCTAGCAGTGGAGTTGGGGCCGCACGGGATCAGAGTGAATGCGATATCGCCGTTCGCGACGGTGACGCCGATGCTGCTGCCGTCGAAGGACGTGGAACAGAAGAAGGCAATGGAGGCGTTCATTTCTCTGTCAGGGAACTTGAAAGGGGCAGTAATGGAGGCTGAAGATGTAGCCAAAGCAGCTGTGTACTTAGCGAGTGATGAGTCCAAATATGTGAGCGGATTGAATCTGGTGGTTGATGGTGGCTTCAGTCTTACCAATCCCAGCTTTGAATTTAATGCCAAAGCTTATGGACAGTGA

Coding sequence (CDS)

ATGACTTCCAATGGCGTTTCTGCATCAATCTCCAAAAGATTGGCCGGAAAAGTGGCTCTGATAACCGGCGGAGCAAGTGGAATCGGCGAGTCGACGGTGAGGCTGTTTGTCGAGAACGGAGCGAAAGTGGTGATCGCCGACGTCCAGGACGACCTCGCCGCCGCCCTCTGCAAGGAACTCAGCGACGCCGGCGGCGACATTTCCTACTTCCACTGCAATGTCACAGACGAATCTGACATCTCGAACGCGGTCGACTTTGCAGTCGACAAGTATGGAAAGCTCGACATCATGTTCAACAATGCTGGCATTCGCGGCGATGTTACCGCCACGACGTTGACCACGGACATGAACGATTTCAAGAACGTCTTCGACGTTAACGTGTTCGGTACTTTTATGGGAGCCAAACACGCCGCCAGGGTCATGGTTCCGGCAAAAACAGGATGCATCCTGTTCACATCGAGCATGGCATCGGTGATATGCAGCGGTAACACACCGGCATATTCGGCGTCGAAGCACGCGATCGTGGGACTGATGAAGACGCTAGCAGTGGAGTTGGGGCCGCACGGGATCAGAGTGAATGCGATATCGCCGTTCGCGACGGTGACGCCGATGCTGCTGCCGTCGAAGGACGTGGAACAGAAGAAGGCAATGGAGGCGTTCATTTCTCTGTCAGGGAACTTGAAAGGGGCAGTAATGGAGGCTGAAGATGTAGCCAAAGCAGCTGTGTACTTAGCGAGTGATGAGTCCAAATATGTGAGCGGATTGAATCTGGTGGTTGATGGTGGCTTCAGTCTTACCAATCCCAGCTTTGAATTTAATGCCAAAGCTTATGGACAGTGA

Protein sequence

MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKELSDAGGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDFKNVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMKTLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAKAAVYLASDESKYVSGLNLVVDGGFSLTNPSFEFNAKAYGQ

Homology

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BLAST of Spg036769 vs. NCBI nr
Match: XP_008437356.1 (PREDICTED: zerumbone synthase [Cucumis melo] >TYJ99091.1 zerumbone synthase [Cucumis melo var. makuwa])

HSP 1 Score: 485.0 bits (1247), Expect = 4.5e-133
Identity = 250/280 (89.29%), Postives = 267/280 (95.36%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           M+SNGVS+SISKRLAGKVALITGGASGIGESTVRLFVENGAKVV+ADVQDDL A LCKEL
Sbjct: 1   MSSNGVSSSISKRLAGKVALITGGASGIGESTVRLFVENGAKVVVADVQDDLGAVLCKEL 60

Query: 61  SDAGG-DISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDF 120
            D  G ++SYFHC+VTDESDISNAVD+AV+KYGKLDIMFNNAGIRGDV ATTLTTDMNDF
Sbjct: 61  DDGTGYNVSYFHCDVTDESDISNAVDYAVEKYGKLDIMFNNAGIRGDVGATTLTTDMNDF 120

Query: 121 KNVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           K VFDVNVFG+FMGAKHAARVM PAKTGCILFTSSMASVICSGN PAY+ASKHAI+GLMK
Sbjct: 121 KKVFDVNVFGSFMGAKHAARVMAPAKTGCILFTSSMASVICSGNAPAYAASKHAIIGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAK 240
           TLAVELGP GIRVNAISP+ATVTPML+PSK+VE+KKAMEAFISLSGNL+GAVMEAEDVAK
Sbjct: 181 TLAVELGPKGIRVNAISPYATVTPMLVPSKNVEEKKAMEAFISLSGNLQGAVMEAEDVAK 240

Query: 241 AAVYLASDESKYVSGLNLVVDGGFSLTNPSFEFNAKAYGQ 280
           AA+YLASDESKYVSGLNLVVDGGFSLTNPSFE NAK YGQ
Sbjct: 241 AALYLASDESKYVSGLNLVVDGGFSLTNPSFELNAKIYGQ 280

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BLAST of Spg036769 vs. NCBI nr
Match: KAG6573007.1 (Zerumbone synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 484.6 bits (1246), Expect = 5.9e-133
Identity = 250/280 (89.29%), Postives = 265/280 (94.64%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           MTSNGVSA+ISKRLAGKVALITGGASGIG STVRLFV+NGAKVVIADVQDDLAAALCKEL
Sbjct: 1   MTSNGVSATISKRLAGKVALITGGASGIGASTVRLFVKNGAKVVIADVQDDLAAALCKEL 60

Query: 61  SDA-GGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDF 120
            DA   D+SYFHC+VT+ESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATT+ TDMNDF
Sbjct: 61  DDATDHDVSYFHCDVTNESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTMNTDMNDF 120

Query: 121 KNVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           K VFDVNVFGTFMGAKHAARVMVPAK+GCILFTSSMASVICSGNTPAYSASKHAIVGLMK
Sbjct: 121 KKVFDVNVFGTFMGAKHAARVMVPAKSGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAK 240
           TLAVELGPHGIRVNAISPFATVTPMLLPS DVEQKKAME  I +SGNLKGA+M+ ED+AK
Sbjct: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSMDVEQKKAMETLIYMSGNLKGAMMDVEDIAK 240

Query: 241 AAVYLASDESKYVSGLNLVVDGGFSLTNPSFEFNAKAYGQ 280
           AA+YLASDESKYVSGLN+V+DGGFSLTNPSFE + KAY Q
Sbjct: 241 AALYLASDESKYVSGLNMVIDGGFSLTNPSFELSVKAYAQ 280

[Close]

BLAST of Spg036769 vs. NCBI nr
Match: XP_022994653.1 (zerumbone synthase-like [Cucurbita maxima])

HSP 1 Score: 484.2 bits (1245), Expect = 7.7e-133
Identity = 250/280 (89.29%), Postives = 263/280 (93.93%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           M+SNGVSA+ISKRLAGKVALITGGASGIG STVRLFVENGAKVVIADVQDDLAAALCKEL
Sbjct: 1   MSSNGVSATISKRLAGKVALITGGASGIGASTVRLFVENGAKVVIADVQDDLAAALCKEL 60

Query: 61  SDA-GGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDF 120
            DA   D+SYFHC+VTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATT+ TDMNDF
Sbjct: 61  DDATNHDVSYFHCDVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTMNTDMNDF 120

Query: 121 KNVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           K VFDVNVFGTFMGAKHAARVMVPAK+GCILFTSSMASVICSGNTPAYSASKHAIVGLMK
Sbjct: 121 KKVFDVNVFGTFMGAKHAARVMVPAKSGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAK 240
           TLAVELGPHGIRVNAISPFA VTPMLLPS D EQKKAME  I LSGNLKGA+M+ ED+AK
Sbjct: 181 TLAVELGPHGIRVNAISPFAAVTPMLLPSMDAEQKKAMETLIYLSGNLKGAMMDVEDIAK 240

Query: 241 AAVYLASDESKYVSGLNLVVDGGFSLTNPSFEFNAKAYGQ 280
           AA+YLASDESKYVSGLN+V+DGGFSLTNPSFE + KAY Q
Sbjct: 241 AALYLASDESKYVSGLNMVIDGGFSLTNPSFELSVKAYAQ 280

[Close]

BLAST of Spg036769 vs. NCBI nr
Match: XP_023542445.1 (zerumbone synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 479.9 bits (1234), Expect = 1.4e-131
Identity = 247/280 (88.21%), Postives = 264/280 (94.29%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           M+SNGVSA+ISKRLAGKVALITGGASGIG STVRLFV+NGAKVVIADVQDDLAAALCKEL
Sbjct: 1   MSSNGVSATISKRLAGKVALITGGASGIGASTVRLFVKNGAKVVIADVQDDLAAALCKEL 60

Query: 61  SDA-GGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDF 120
            DA   D+SYFHC+VT+ESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATT+ TDMNDF
Sbjct: 61  DDATDHDVSYFHCDVTNESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTMNTDMNDF 120

Query: 121 KNVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           K VFDVNVFGTFMGAKHAARVMVPAK+GCILFTSSMASVICSGNTPAYSASKHAIVGLMK
Sbjct: 121 KKVFDVNVFGTFMGAKHAARVMVPAKSGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAK 240
           TLAVELGPHGIRVNA+SPFA VTPMLLPS DVEQKKAME  I +SGNLKGA+M+ ED+AK
Sbjct: 181 TLAVELGPHGIRVNAMSPFAAVTPMLLPSMDVEQKKAMETLIYMSGNLKGAMMDVEDIAK 240

Query: 241 AAVYLASDESKYVSGLNLVVDGGFSLTNPSFEFNAKAYGQ 280
           AA+YLASDESKYVSGLN+V+DGGFSLTNPSFE + KAY Q
Sbjct: 241 AALYLASDESKYVSGLNMVIDGGFSLTNPSFELSVKAYAQ 280

[Close]

BLAST of Spg036769 vs. NCBI nr
Match: XP_038895453.1 (secoisolariciresinol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 479.6 bits (1233), Expect = 1.9e-131
Identity = 248/279 (88.89%), Postives = 262/279 (93.91%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           M+SNGVS+SISKRLAGKVA+ITGGASGIGESTVRLFVENGAKVVI DVQDDLAAALCK L
Sbjct: 1   MSSNGVSSSISKRLAGKVAIITGGASGIGESTVRLFVENGAKVVIVDVQDDLAAALCKVL 60

Query: 61  SDAGGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDFK 120
            D   D+SYFHC++TDES ISNAVDF V KY KLDIMFNNAGIRG+VTATT+TTDMNDFK
Sbjct: 61  GD---DVSYFHCDITDESGISNAVDFTVQKYRKLDIMFNNAGIRGNVTATTMTTDMNDFK 120

Query: 121 NVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMKT 180
            VFDVNVFG+FMGAKHA RVM PAKTGCILFTSSMASVICSGN PAYSASKHA+VGLMKT
Sbjct: 121 KVFDVNVFGSFMGAKHAVRVMAPAKTGCILFTSSMASVICSGNAPAYSASKHAVVGLMKT 180

Query: 181 LAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAKA 240
           LAVELGP+GIRVNAISPFATVTPMLLPSKDV QKKAMEA IS+SGNLKGA+MEAEDVAKA
Sbjct: 181 LAVELGPYGIRVNAISPFATVTPMLLPSKDVGQKKAMEAVISMSGNLKGAIMEAEDVAKA 240

Query: 241 AVYLASDESKYVSGLNLVVDGGFSLTNPSFEFNAKAYGQ 280
           A+YLASDESKYVSGLNLVVDGGFSLTNPSFEFN KAYGQ
Sbjct: 241 ALYLASDESKYVSGLNLVVDGGFSLTNPSFEFNTKAYGQ 276

[Close]

BLAST of Spg036769 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 8.1e-69
Identity = 138/266 (51.88%), Postives = 182/266 (68.42%), Query Frame = 0


Query: 13  RLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKELSDAGGD--ISYF 72
           RL GKVAL+TGGASGIGES  RLF+E+GAK+ I DVQD+L   + + L   GGD    YF
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRL---GGDPHACYF 61

Query: 73  HCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDFKNVFDVNVFGT 132
           HC+VT E D+  AVDF  +KYG +DIM NNAGI GD        D N+FK VFD+NV G 
Sbjct: 62  HCDVTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGV 121

Query: 133 FMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMKTLAVELGPHGI 192
           F+G KHAAR+M+P   G I+  +S++SVI       Y+ +KHA+VGL K++A ELG HGI
Sbjct: 122 FLGMKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGI 181

Query: 193 RVNAISPFATVTPMLLP--SKDVEQKKAMEAFISL---SGNLKGAVMEAEDVAKAAVYLA 252
           RVN +SP+A  T + +P   +   Q+ A+  F++    + NLKG  +   DVA+A +YLA
Sbjct: 182 RVNCVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLA 241

Query: 253 SDESKYVSGLNLVVDGGFSLTNPSFE 272
           ++ESKYVSGLNLV+DGGFS+ N + +
Sbjct: 242 TEESKYVSGLNLVIDGGFSIANHTLQ 264

[Close]

BLAST of Spg036769 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 5.8e-67
Identity = 136/271 (50.18%), Postives = 180/271 (66.42%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           M S    AS + RL  KVA+ITGGA GIGE+T +LFV  GAKVVIAD+ DD    +C  +
Sbjct: 1   MGSTSTPASSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNI 60

Query: 61  SDAGGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDFK 120
             +   IS+ HC+VT + D+ N VD  + K+GKLDIMF N G+      + L     DFK
Sbjct: 61  G-SPDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFK 120

Query: 121 NVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVIC-SGNTPAYSASKHAIVGLMK 180
            V D+NV+G F+ AKHAARVM+PAK G I+FT+S++S     G +  Y+A+KHA++GL  
Sbjct: 121 RVMDINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTT 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAK 240
           +L  ELG HGIRVN +SP+   +P+L     V+  + +E     + NLKG ++ AEDVA 
Sbjct: 181 SLCTELGQHGIRVNCVSPYVVASPLLTDVFGVDSSR-VEELAHQAANLKGILLRAEDVAD 240

Query: 241 AAVYLASDESKYVSGLNLVVDGGFSLTNPSF 271
           A  YLA DESKYVSGLNLV+DGG++ TNP+F
Sbjct: 241 AVAYLAGDESKYVSGLNLVIDGGYTRTNPAF 269

[Close]

BLAST of Spg036769 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.3e-66
Identity = 135/262 (51.53%), Postives = 182/262 (69.47%), Query Frame = 0


Query: 6   VSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKELSDAGG 65
           V  +I++RL GKVALITGGASGIGE+T +LF ++GAKV IADVQD+L  ++ + +  +  
Sbjct: 7   VLTAIARRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNS 66

Query: 66  DISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDFKNVFDV 125
             +Y HC+VT+E  + NAVD  V  YGKLDIMF+NAGI        +  +  DF+ VF V
Sbjct: 67  --TYIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSV 126

Query: 126 NVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMKTLAVEL 185
           NV G F+  KHAARVM+PA++G I+ T+S++S +  G++ AY  SKHA++GL + LAVEL
Sbjct: 127 NVTGVFLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVEL 186

Query: 186 GPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAKAAVYLA 245
           G  GIRVN +SPF   T +      ++ ++  E  I+ +GNLKG     EDVA AA+YLA
Sbjct: 187 GQFGIRVNCLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAALYLA 246

Query: 246 SDESKYVSGLNLVVDGGFSLTN 268
           SDE+KYVSG NL +DGGFS+ N
Sbjct: 247 SDEAKYVSGHNLFIDGGFSVCN 266

[Close]

BLAST of Spg036769 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.9e-65
Identity = 134/271 (49.45%), Postives = 179/271 (66.05%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           M S     S + RL  KVA+ITGGA GIGE+T +LFV  GAKVVIAD+ DD    +C  +
Sbjct: 1   MGSTSTPDSSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNI 60

Query: 61  SDAGGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDFK 120
             +   IS+ HC+VT + D+ N VD  + K+GKLDIMF N G+      + L     DFK
Sbjct: 61  G-SPDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFK 120

Query: 121 NVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVIC-SGNTPAYSASKHAIVGLMK 180
            V D+NV+G F+ AKHAARVM+PAK G I+FT+S++S     G +  Y+A+KHA++GL  
Sbjct: 121 RVMDINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTT 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAK 240
           +L  ELG +GIRVN +SP+   +P+L     V+  + +E     + NLKG ++ AEDVA 
Sbjct: 181 SLCTELGEYGIRVNCVSPYIVASPLLTDVFGVDSSR-VEELAHQAANLKGTLLRAEDVAD 240

Query: 241 AAVYLASDESKYVSGLNLVVDGGFSLTNPSF 271
           A  YLA DESKYVSGLNLV+DGG++ TNP+F
Sbjct: 241 AVAYLAGDESKYVSGLNLVIDGGYTRTNPAF 269

[Close]

BLAST of Spg036769 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 4.2e-65
Identity = 137/271 (50.55%), Postives = 188/271 (69.37%), Query Frame = 0


Query: 3   SNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKELSD 62
           S+ VSA  +++L GKVA+ITGGASGIG  T RLFV++GA+VV+AD+QD+L A+L  EL  
Sbjct: 5   SSHVSAD-ARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGP 64

Query: 63  AGGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDFKNV 122
                SY HC+VT+E D++ AVD AV ++GKLD+MFNNAG+ G            DF+ V
Sbjct: 65  DAS--SYVHCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERV 124

Query: 123 FDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMKTLA 182
             VN+ G F+G KHAARVM PA+ G I+ T+S++S +    + AY+ SKHA+VG  +  A
Sbjct: 125 LAVNLVGPFLGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAA 184

Query: 183 VELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGA-VMEAEDVAKAA 242
            ELG HGIRVN +SP    TP+   +  ++  +A+EA ++ S NLKGA  ++A+D+A AA
Sbjct: 185 GELGRHGIRVNCVSPAGVATPLARAAMGMDD-EAIEAIMANSANLKGAGALKADDIAAAA 244

Query: 243 VYLASDESKYVSGLNLVVDGGFSLTNPSFEF 273
           ++LASD+ +YVSG NL VDGG S+ N SF F
Sbjct: 245 LFLASDDGRYVSGQNLRVDGGLSVVNSSFGF 271

[Close]

BLAST of Spg036769 vs. ExPASy TrEMBL
Match: A0A5D3BK37 (Zerumbone synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002950 PE=4 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.2e-133
Identity = 250/280 (89.29%), Postives = 267/280 (95.36%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           M+SNGVS+SISKRLAGKVALITGGASGIGESTVRLFVENGAKVV+ADVQDDL A LCKEL
Sbjct: 1   MSSNGVSSSISKRLAGKVALITGGASGIGESTVRLFVENGAKVVVADVQDDLGAVLCKEL 60

Query: 61  SDAGG-DISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDF 120
            D  G ++SYFHC+VTDESDISNAVD+AV+KYGKLDIMFNNAGIRGDV ATTLTTDMNDF
Sbjct: 61  DDGTGYNVSYFHCDVTDESDISNAVDYAVEKYGKLDIMFNNAGIRGDVGATTLTTDMNDF 120

Query: 121 KNVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           K VFDVNVFG+FMGAKHAARVM PAKTGCILFTSSMASVICSGN PAY+ASKHAI+GLMK
Sbjct: 121 KKVFDVNVFGSFMGAKHAARVMAPAKTGCILFTSSMASVICSGNAPAYAASKHAIIGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAK 240
           TLAVELGP GIRVNAISP+ATVTPML+PSK+VE+KKAMEAFISLSGNL+GAVMEAEDVAK
Sbjct: 181 TLAVELGPKGIRVNAISPYATVTPMLVPSKNVEEKKAMEAFISLSGNLQGAVMEAEDVAK 240

Query: 241 AAVYLASDESKYVSGLNLVVDGGFSLTNPSFEFNAKAYGQ 280
           AA+YLASDESKYVSGLNLVVDGGFSLTNPSFE NAK YGQ
Sbjct: 241 AALYLASDESKYVSGLNLVVDGGFSLTNPSFELNAKIYGQ 280

[Close]

BLAST of Spg036769 vs. ExPASy TrEMBL
Match: A0A1S3AUF2 (zerumbone synthase OS=Cucumis melo OX=3656 GN=8D10 PE=4 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.2e-133
Identity = 250/280 (89.29%), Postives = 267/280 (95.36%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           M+SNGVS+SISKRLAGKVALITGGASGIGESTVRLFVENGAKVV+ADVQDDL A LCKEL
Sbjct: 1   MSSNGVSSSISKRLAGKVALITGGASGIGESTVRLFVENGAKVVVADVQDDLGAVLCKEL 60

Query: 61  SDAGG-DISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDF 120
            D  G ++SYFHC+VTDESDISNAVD+AV+KYGKLDIMFNNAGIRGDV ATTLTTDMNDF
Sbjct: 61  DDGTGYNVSYFHCDVTDESDISNAVDYAVEKYGKLDIMFNNAGIRGDVGATTLTTDMNDF 120

Query: 121 KNVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           K VFDVNVFG+FMGAKHAARVM PAKTGCILFTSSMASVICSGN PAY+ASKHAI+GLMK
Sbjct: 121 KKVFDVNVFGSFMGAKHAARVMAPAKTGCILFTSSMASVICSGNAPAYAASKHAIIGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAK 240
           TLAVELGP GIRVNAISP+ATVTPML+PSK+VE+KKAMEAFISLSGNL+GAVMEAEDVAK
Sbjct: 181 TLAVELGPKGIRVNAISPYATVTPMLVPSKNVEEKKAMEAFISLSGNLQGAVMEAEDVAK 240

Query: 241 AAVYLASDESKYVSGLNLVVDGGFSLTNPSFEFNAKAYGQ 280
           AA+YLASDESKYVSGLNLVVDGGFSLTNPSFE NAK YGQ
Sbjct: 241 AALYLASDESKYVSGLNLVVDGGFSLTNPSFELNAKIYGQ 280

[Close]

BLAST of Spg036769 vs. ExPASy TrEMBL
Match: A0A6J1K5R5 (zerumbone synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111490301 PE=4 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 3.7e-133
Identity = 250/280 (89.29%), Postives = 263/280 (93.93%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           M+SNGVSA+ISKRLAGKVALITGGASGIG STVRLFVENGAKVVIADVQDDLAAALCKEL
Sbjct: 1   MSSNGVSATISKRLAGKVALITGGASGIGASTVRLFVENGAKVVIADVQDDLAAALCKEL 60

Query: 61  SDA-GGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDF 120
            DA   D+SYFHC+VTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATT+ TDMNDF
Sbjct: 61  DDATNHDVSYFHCDVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTMNTDMNDF 120

Query: 121 KNVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           K VFDVNVFGTFMGAKHAARVMVPAK+GCILFTSSMASVICSGNTPAYSASKHAIVGLMK
Sbjct: 121 KKVFDVNVFGTFMGAKHAARVMVPAKSGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAK 240
           TLAVELGPHGIRVNAISPFA VTPMLLPS D EQKKAME  I LSGNLKGA+M+ ED+AK
Sbjct: 181 TLAVELGPHGIRVNAISPFAAVTPMLLPSMDAEQKKAMETLIYLSGNLKGAMMDVEDIAK 240

Query: 241 AAVYLASDESKYVSGLNLVVDGGFSLTNPSFEFNAKAYGQ 280
           AA+YLASDESKYVSGLN+V+DGGFSLTNPSFE + KAY Q
Sbjct: 241 AALYLASDESKYVSGLNMVIDGGFSLTNPSFELSVKAYAQ 280

[Close]

BLAST of Spg036769 vs. ExPASy TrEMBL
Match: A0A6J1GSR4 (zerumbone synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111457191 PE=4 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.2e-131
Identity = 247/280 (88.21%), Postives = 264/280 (94.29%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           M+SNGVSA+ISKRLAGKVALITGGASGIG STVRLFV+NGAKVVIADVQDDLAAALCKEL
Sbjct: 1   MSSNGVSATISKRLAGKVALITGGASGIGASTVRLFVKNGAKVVIADVQDDLAAALCKEL 60

Query: 61  SDA-GGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDF 120
           +DA   D+SYFHC+VT+ESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTA T+ TDMNDF
Sbjct: 61  NDATDHDVSYFHCDVTNESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTAMTMNTDMNDF 120

Query: 121 KNVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180
           K VFDVNVFGTFMGAKHAARVMVPAK+GCILFTSSMASVICSGNTPAYSASKHAIVGLMK
Sbjct: 121 KKVFDVNVFGTFMGAKHAARVMVPAKSGCILFTSSMASVICSGNTPAYSASKHAIVGLMK 180

Query: 181 TLAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAK 240
           TLAVELGPHGIRVNAISPFA VTPMLLPS DVEQKKAME  I +SGNLKGA+M+ ED+AK
Sbjct: 181 TLAVELGPHGIRVNAISPFAAVTPMLLPSMDVEQKKAMETLIYMSGNLKGAMMDVEDIAK 240

Query: 241 AAVYLASDESKYVSGLNLVVDGGFSLTNPSFEFNAKAYGQ 280
           AA+YLASDESKYVSGLN+V+DGGFSLTNPSFE + KAY Q
Sbjct: 241 AALYLASDESKYVSGLNMVIDGGFSLTNPSFELSVKAYAQ 280

[Close]

BLAST of Spg036769 vs. ExPASy TrEMBL
Match: A0A0A0LTA0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G044880 PE=4 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 4.5e-131
Identity = 243/279 (87.10%), Postives = 263/279 (94.27%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           M+SNGVS+SISKRLAGKVALITGGASGIGESTVRLFVENGAKVV+ADVQDDL A LCKEL
Sbjct: 1   MSSNGVSSSISKRLAGKVALITGGASGIGESTVRLFVENGAKVVVADVQDDLGAVLCKEL 60

Query: 61  SDAGGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDFK 120
            D G ++SYFHC+VTDESDISNAVD+AV+KYGKLDIMFNNAGIRGDV +TTLT DMNDF+
Sbjct: 61  DDTGFNVSYFHCDVTDESDISNAVDYAVEKYGKLDIMFNNAGIRGDVGSTTLTADMNDFR 120

Query: 121 NVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMKT 180
            VFDVNVFG+FMGAKHAARVM PAKTGCILFTSSMASVICSGN PAY+ASKHAI+GLMKT
Sbjct: 121 KVFDVNVFGSFMGAKHAARVMAPAKTGCILFTSSMASVICSGNAPAYAASKHAIIGLMKT 180

Query: 181 LAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAKA 240
           LAVELG  GIRVNAISP+ATVTPML+PS++VE+KKAMEAFISLSGNL+GAVMEAEDVAKA
Sbjct: 181 LAVELGSQGIRVNAISPYATVTPMLVPSRNVEEKKAMEAFISLSGNLQGAVMEAEDVAKA 240

Query: 241 AVYLASDESKYVSGLNLVVDGGFSLTNPSFEFNAKAYGQ 280
           A+YL SDESKYVSGLNLVVDGGFSLTNPSFE NAK Y Q
Sbjct: 241 ALYLGSDESKYVSGLNLVVDGGFSLTNPSFELNAKIYAQ 279

[Close]

BLAST of Spg036769 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 236.1 bits (601), Expect = 3.4e-62
Identity = 131/277 (47.29%), Postives = 182/277 (65.70%), Query Frame = 0


Query: 3   SNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL-- 62
           S+  S+  S+RL GKVALITGGA+GIGES VRLF ++GAKV I D+QDDL   +CK L  
Sbjct: 7   SSSYSSLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLR 66

Query: 63  SDAGGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDFK 122
            ++     + H +V  E DISNAVDFAV  +G LDI+ NNAG+ G          +++F+
Sbjct: 67  GESKETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFE 126

Query: 123 NVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMKT 182
             FDVNV G F+  KHAARVM+P K G I+   S+  V+      +Y  SKHA++GL ++
Sbjct: 127 MTFDVNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRS 186

Query: 183 LAVELGPHGIRVNAISPFATVTPMLLPSKDVEQKKAMEAFI------SLSGNLKGAVMEA 242
           +A ELG HGIRVN +SP+A  T + L     E+++  +AF+      + + NLKG  +  
Sbjct: 187 VAAELGQHGIRVNCVSPYAVATKLALAHLP-EEERTEDAFVGFRNFAAANANLKGVELTV 246

Query: 243 EDVAKAAVYLASDESKYVSGLNLVVDGGFSLTNPSFE 272
           +DVA A ++LASD+S+Y+SG NL++DGGF+ TN SF+
Sbjct: 247 DDVANAVLFLASDDSRYISGDNLMIDGGFTCTNHSFK 282

[Close]

BLAST of Spg036769 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 221.9 bits (564), Expect = 6.6e-58
Identity = 132/264 (50.00%), Postives = 171/264 (64.77%), Query Frame = 0


Query: 12  KRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKELSD--AGGDISY 71
           KRL GKVA+ITGGA GIG++TV LF  +GA VVIADV +   ++L K LS       +++
Sbjct: 30  KRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAF 89

Query: 72  FHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVT--ATTLTTDMNDFKNVFDVNV 131
             C+V+ E+D+ N V+  V +YG+LDI+FNNAG+ GD     + L  D ++F +V  VNV
Sbjct: 90  ISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNV 149

Query: 132 FGTFMGAKHAARVMVP-AKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMKTLAVELG 191
            G  +G KH AR M+     GCI+ T+S+A V+      AY+ASKHAIVGL K  A ELG
Sbjct: 150 RGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELG 209

Query: 192 PHGIRVNAISPFATVTPMLL------PSKDVEQK--KAMEAFISLSGNLKGAVMEAEDVA 251
            +GIRVN ISPF   T ML+         DVE    + ME F+    NLKG  + A D+A
Sbjct: 210 KYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIA 269

Query: 252 KAAVYLASDESKYVSGLNLVVDGG 263
           +AA+YLASDESKYV+G NLVVDGG
Sbjct: 270 EAALYLASDESKYVNGHNLVVDGG 293

[Close]

BLAST of Spg036769 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 219.9 bits (559), Expect = 2.5e-57
Identity = 124/260 (47.69%), Postives = 171/260 (65.77%), Query Frame = 0


Query: 11  SKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKELSDAGGDISYF 70
           SK+L GKVALITGGASG+G++T   F+ +GA+VVIAD+  +      KEL   G +  + 
Sbjct: 38  SKKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKEL---GSEAEFV 97

Query: 71  HCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLT-TDMNDFKNVFDVNVFG 130
            C+VT E+DI+ AV+  V++YGKLD+M+NNAGI G +T  +++  DM +F+ V  +NVFG
Sbjct: 98  RCDVTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRINVFG 157

Query: 131 TFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMKTLAVELGPHG 190
              G KHAA+ M+PA++GCIL TSS+A V       +Y+ SK    G++K+ A EL  HG
Sbjct: 158 VVSGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHG 217

Query: 191 IRVNAISPFATVTPMLLP-----SKDVEQKKAMEAFISLSGNLKGAVMEAEDVAKAAVYL 250
           +R+N ISP    TP+ L         V ++K  E  +   G LKGA  E  DVAKAA+YL
Sbjct: 218 VRINCISPGTVATPLTLSYLQKVFPKVSEEKLRET-VKGMGELKGAECEEADVAKAALYL 277

Query: 251 ASDESKYVSGLNLVVDGGFS 265
           AS++ KYV+G NLVVDGG +
Sbjct: 278 ASNDGKYVTGHNLVVDGGMT 293

[Close]

BLAST of Spg036769 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 218.0 bits (554), Expect = 9.5e-57
Identity = 127/269 (47.21%), Postives = 172/269 (63.94%), Query Frame = 0


Query: 1   MTSNGVSASISKRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKEL 60
           ++S+  S S S++L GKVALITGGASGIG++T   F+ +GAKV+IAD+Q  +     +EL
Sbjct: 65  ISSSSSSHSTSRKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQEL 124

Query: 61  SDAGGDISYFHCNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDFK 120
              G   +YF C+VT ESDI+NAVDFAV  + KLDIM+NNAGI      + +  D+N F 
Sbjct: 125 ---GPSCAYFPCDVTKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFD 184

Query: 121 NVFDVNVFGTFMGAKHAARVMVPAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMKT 180
            V + NV G   G KHAARVM+P  +G I+   S+  ++       YS SK A++G++++
Sbjct: 185 KVINTNVRGVMAGIKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRS 244

Query: 181 LAVELGPHGIRVNAISPFATVTPMLLPS-----KDVEQKKAMEAFISLSGNLKGAVMEAE 240
            A EL  H IRVN ISPFA  T  ++         V+  + ++  +  +G L G V E  
Sbjct: 245 TASELCKHRIRVNCISPFAITTSFVMDEMRQIYPGVDDSRLIQ-IVQSTGVLNGEVCEPT 304

Query: 241 DVAKAAVYLASDESKYVSGLNLVVDGGFS 265
           DVA AAVYLASD+SKYV+G NLVVDGGF+
Sbjct: 305 DVANAAVYLASDDSKYVNGHNLVVDGGFT 329

[Close]

BLAST of Spg036769 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 214.5 bits (545), Expect = 1.1e-55
Identity = 119/258 (46.12%), Postives = 170/258 (65.89%), Query Frame = 0


Query: 12  KRLAGKVALITGGASGIGESTVRLFVENGAKVVIADVQDDLAAALCKELSDAGGDISYFH 71
           KRL GK+ +ITGGASGIG  +VRLF E+GA+VVI DVQD+L   +   + +     SY+H
Sbjct: 4   KRLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGE--DKASYYH 63

Query: 72  CNVTDESDISNAVDFAVDKYGKLDIMFNNAGIRGDVTATTLTTDMNDFKNVFDVNVFGTF 131
           C+VT+E+++ NAV F V+KYGKLD++F+NAG+  +   + L  ++N+      +N+ GT 
Sbjct: 64  CDVTNETEVENAVKFTVEKYGKLDVLFSNAGVI-EPFVSILDLNLNELDRTIAINLRGTA 123

Query: 132 MGAKHAARVMV-PAKTGCILFTSSMASVICSGNTPAYSASKHAIVGLMKTLAVELGPHGI 191
              KHAAR MV     G I+ T+S+A+ I       Y+ SKH ++GL+K+ +  LG +GI
Sbjct: 124 AFIKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGI 183

Query: 192 RVNAISPFATVTPMLLPSKDVEQKKAMEAFISLSGNLKGAVMEAEDVAKAAVYLASDESK 251
           RVN ++PF   TP++     +E    +E   S S NLKG V++A  VA+AA++LASDES 
Sbjct: 184 RVNGVAPFGVATPLVCNGFKME-PNVVEQNTSASANLKGIVLKARHVAEAALFLASDESA 243

Query: 252 YVSGLNLVVDGGFSLTNP 269
           YVSG NL VDGG+S+  P
Sbjct: 244 YVSGQNLAVDGGYSVVKP 257

The following BLAST results are available for this feature:

BLAST of Spg036769 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008437356.1	4.5e-133	89.29	PREDICTED: zerumbone synthase [Cucumis melo] >TYJ99091.1 zerumbone synthase [Cuc...	[more]
KAG6573007.1	5.9e-133	89.29	Zerumbone synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022994653.1	7.7e-133	89.29	zerumbone synthase-like [Cucurbita maxima]	[more]
XP_023542445.1	1.4e-131	88.21	zerumbone synthase-like [Cucurbita pepo subsp. pepo]	[more]
XP_038895453.1	1.9e-131	88.89	secoisolariciresinol dehydrogenase-like [Benincasa hispida]	[more]

BLAST of Spg036769 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F1SWA0	8.1e-69	51.88	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]
A3F5F0	5.8e-67	50.18	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
Q94KL7	1.3e-66	51.53	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q94KL8	1.9e-65	49.45	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
Q7FAE1	4.2e-65	50.55	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]

BLAST of Spg036769 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BK37	2.2e-133	89.29	Zerumbone synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G0...	[more]
A0A1S3AUF2	2.2e-133	89.29	zerumbone synthase OS=Cucumis melo OX=3656 GN=8D10 PE=4 SV=1	[more]
A0A6J1K5R5	3.7e-133	89.29	zerumbone synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111490301 PE=4 SV=1	[more]
A0A6J1GSR4	1.2e-131	88.21	zerumbone synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111457191 PE=4 SV=1	[more]
A0A0A0LTA0	4.5e-131	87.10	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G044880 PE=4 SV=1	[more]

BLAST of Spg036769 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52340.1	3.4e-62	47.29	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G51680.1	6.6e-58	50.00	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	2.5e-57	47.69	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G03140.1	9.5e-57	47.21	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	1.1e-55	46.12	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 18..35 score: 49.15 coord: 141..157 score: 28.72 coord: 92..103 score: 58.12 coord: 167..186 score: 45.15 coord: 227..247 score: 33.43 coord: 188..205 score: 42.36
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 92..103 score: 51.1 coord: 147..155 score: 37.09 coord: 167..186 score: 40.72
None	No IPR available	GENE3D	3.40.50.720		coord: 3..277 e-value: 2.6E-83 score: 281.2
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 23..264 e-value: 1.3E-66 score: 224.5
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 14..266 e-value: 3.0E-22 score: 76.8
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 20..207 e-value: 6.6E-19 score: 66.0
None	No IPR available	PANTHER	PTHR43180:SF71	SECOISOLARICIRESINOL DEHYDROGENASE-LIKE	coord: 8..272
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 8..272
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 154..182
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 16..265

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg036769.1	Spg036769.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity