Spg036709 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg036709
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectate lyase
Location	scaffold5: 45853840 .. 45856607 (+)
RNA-Seq Expression	Spg036709
Synteny	Spg036709

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTCTTTTCTTTCATTTCTCGTAATGAAAACTTGGTTTCTTACAAATAAAAAATAATAAAAAAAGTCTTAATTTAATTATTATTTCAAACAACCCAAGATCTTATAATCCACAAATTTGCACAAGTAAATGGAAAGTCCCAAGACCACAATTCCATGAAAATGGATAAAGAAAGCAAATAAGAAATTGGCCATTGAAGGGGATGGCTCCCCTTTATTTCCTCTCCATTTCTCATTCTCTTATTTAATGAAGCTCTAGATTTATCATTTTCTACATTTTAGTTATATAACCATGTCGACTTCATCCCAAACTCAAATGGATTATTGAGCATCAACAAAAGCTTACAAAGAAAAATCTCCAAAAGATTAACAAAGCAGAAAGAGAAGATGAGAGAGACAGAAACCACCATTGTTGTGCTTAAGCTGCTAATGATATCTGCTTTTGTAATGATATTCCCAACCCTTAATGCTAATATTGCAAATTTTGATGAGGTATGGCAGAAAAGAGCTGAAGAAGCCAAGAAGGCTGCCTATGAAGCCTACCAACCCCATCCAGAAGAAGTCACTAATAATTTCAACCAGCAAGTTCACAGGTAACCCCATTTCTTCATTAGCAAATAAATCAGTTTTCTGTTAAATTTGAACATTAAGTATAAATCTGCATTTAGTTAAGATAAGAAGACAATGTCTATGTTGTATCTGATGGTGAATAATTTTGTCTTTCATTGTGAGACAGTTCCTTCAATGGTGCAAATAACACAAGGAGACATTTGAAGAAGTACAATGGTCCATGTTTAGCTACGAATCCTATCGACCGATGTTGGAGATGTGATAAACATTGGGCTAAAAACCGCAAGAAGCTAGCTGATTGTGCCCTTGGCTTTGGCCGTGGAACGACTGGAGGCAAAGATGGAAGATATTACGTGGTGCAAGATTCAAGTGACAACGACTTGCTTAATCCTAAACCGGGAACCTTGCGCTACGGTGTGATACAGGATAGACCACTTTGGATTATATTTGCACGTGACATGGTGATTAGGCTGAGTGAAGAGCTTATAATGACCGATAACAAGACATTAGATGCACGTGGTGCCAATGTGCACATCGCTAATGGCGGTCAAATCACTCTACAATTTGTGAAGAACATAATCATCCATAATCTGCACATTCATGATATCAAGGCTGGCAATGGAGGCCTTGTAAGAGACTCGGTGAACCACTATGGATTTCGAACACCGAGTGATGGTGATGGCATCTCGATGTTTGGAGCCTCAAAGGTTTGGATAGATCATGTTTCAATGTCTAATTGTCAAGATGGACTTATTGATGCAGTCATGGCATCTACTGGCATTACCATCTCCAACTGTCACTTCACCCACCATAACGATGTAAGCTCCTCAAACCATAAAACAAAGATAACATAATTTTTTCAACTTCAACATAGCTCAATTGGTTGAGACATATACTCTTAACCAAGAGATTAGAGGTTCAAATCCCCATACCTCACATATTGTTAAACTCGTTAAAAAATTGCGTAGCTTTAGATTTCTCTATATCGATGTTGATGACTGATAAATTCTTTTTGTGCACGGAAGGTGATACTATTAGGTGCAAGTGATAGCTACTCGAACGATCAGATTCTGCAGGTAACTCTTGCATTTAATCACTTTGGTAAAGGGTTGGTGCAAAGGATGCCGAGATGCCGATGGGGGTTCGTCCATGTAGTCAATAATGACTACACTCACTGGCTTATGTACGCCATTGGCGGCAGCCACAATCCCACCATTGTCAGCCAGGGCAATCGCTTCATCGCCCCACCAGACCCAAATTGCAAAGAGGTATGAAGAAGACAAAGATTAGATCACAAATATCACTTATAATCACACAGGCAGATCCTAAAGAGGTTGCTCAAGTCTTAACCATTAAAACCAACCTCAGGATTGATCAAAGACAAAGAAACAATCTTTCACTTTCTTTATATGTCCAAATGATTAGAGCAATGTACTTGACACACAAGTTTGAGCAATTATTGTTGGGTTGATCCAGGTGACGAAGAGGGCGTACGCACCAGAAAGCGAGTGGAAATCGTGGAACTGGAGATCAGAGGGAGATCTGATGATGAATGGAGCTTTCTTCGTCCAATCTGGGAGCCCCATCAGGAAATACTCCAAGAAAGATGTTATCCATGCAAAACCTGGGACCTTCGTGAGCAGGCTTACACGATTCTCTGGGCCTTTGAAATGCATAAGGAACAAGCCGTGTTAGTGAAGGTTTATTGGAGAATTTTTGACTATGTATATGAATTACCTAAAGCTCAACCGAACCCTGTAATCAACAGAGGTTTTAGCAGTAAAGATTCAGATAGAAACTAAACCTACAGAACTATGGCAGCTTTTGCCTTCATAAAGTTTTGTGAATTTATGTGATATTATGTAAAATATTATTGGCTATCCATCTTAAAGTTGCTTCTTTTCCATATCTATTTTTCTTGCATCACTTTAGTGAACTTGATCAAGAGATGATGCAGATATACTTTAACATCATGCATTTGAAATGTGATATATATGGAACCAATGAAAGGTAGATGAAATAGATAGTCAAGGAGCTGATTTTTTTTTTTTTTTGTTTTTTGGTTAAAAAATGAAAGGGAAAAAGTGAGATAGTAAAGAGAGAGACGTTACCTACTTGTTACACAATAATTTAAATGATTTCTTATATGTTTGTATGAAATAGATTCTTGAACTTTAAAAATATCAAATAGATCTTTGAG

mRNA sequence

ATGAGAGAGACAGAAACCACCATTGTTGTGCTTAAGCTGCTAATGATATCTGCTTTTGTAATGATATTCCCAACCCTTAATGCTAATATTGCAAATTTTGATGAGGTATGGCAGAAAAGAGCTGAAGAAGCCAAGAAGGCTGCCTATGAAGCCTACCAACCCCATCCAGAAGAAGTCACTAATAATTTCAACCAGCAAGTTCACAGTTCCTTCAATGGTGCAAATAACACAAGGAGACATTTGAAGAAGTACAATGGTCCATGTTTAGCTACGAATCCTATCGACCGATGTTGGAGATGTGATAAACATTGGGCTAAAAACCGCAAGAAGCTAGCTGATTGTGCCCTTGGCTTTGGCCGTGGAACGACTGGAGGCAAAGATGGAAGATATTACGTGGTGCAAGATTCAAGTGACAACGACTTGCTTAATCCTAAACCGGGAACCTTGCGCTACGGTGTGATACAGGATAGACCACTTTGGATTATATTTGCACGTGACATGGTGATTAGGCTGAGTGAAGAGCTTATAATGACCGATAACAAGACATTAGATGCACGTGGTGCCAATGTGCACATCGCTAATGGCGGTCAAATCACTCTACAATTTGTGAAGAACATAATCATCCATAATCTGCACATTCATGATATCAAGGCTGGCAATGGAGGCCTTGTAAGAGACTCGGTGAACCACTATGGATTTCGAACACCGAGTGATGGTGATGGCATCTCGATGTTTGGAGCCTCAAAGGTTTGGATAGATCATGTTTCAATGTCTAATTGTCAAGATGGACTTATTGATGCAGTCATGGCATCTACTGGCATTACCATCTCCAACTGTCACTTCACCCACCATAACGATGTGATACTATTAGGTGCAAGTGATAGCTACTCGAACGATCAGATTCTGCAGGTAACTCTTGCATTTAATCACTTTGGTAAAGGGTTGGTGCAAAGGATGCCGAGATGCCGATGGGGGTTCGTCCATGTAGTCAATAATGACTACACTCACTGGCTTATGTACGCCATTGGCGGCAGCCACAATCCCACCATTGTCAGCCAGGGCAATCGCTTCATCGCCCCACCAGACCCAAATTGCAAAGAGGTGACGAAGAGGGCGTACGCACCAGAAAGCGAGTGGAAATCGTGGAACTGGAGATCAGAGGGAGATCTGATGATGAATGGAGCTTTCTTCGTCCAATCTGGGAGCCCCATCAGGAAATACTCCAAGAAAGATGTTATCCATGCAAAACCTGGGACCTTCGTGAGCAGGCTTACACGATTCTCTGGGCCTTTGAAATGCATAAGGAACAAGCCGTGTTAG

Coding sequence (CDS)

ATGAGAGAGACAGAAACCACCATTGTTGTGCTTAAGCTGCTAATGATATCTGCTTTTGTAATGATATTCCCAACCCTTAATGCTAATATTGCAAATTTTGATGAGGTATGGCAGAAAAGAGCTGAAGAAGCCAAGAAGGCTGCCTATGAAGCCTACCAACCCCATCCAGAAGAAGTCACTAATAATTTCAACCAGCAAGTTCACAGTTCCTTCAATGGTGCAAATAACACAAGGAGACATTTGAAGAAGTACAATGGTCCATGTTTAGCTACGAATCCTATCGACCGATGTTGGAGATGTGATAAACATTGGGCTAAAAACCGCAAGAAGCTAGCTGATTGTGCCCTTGGCTTTGGCCGTGGAACGACTGGAGGCAAAGATGGAAGATATTACGTGGTGCAAGATTCAAGTGACAACGACTTGCTTAATCCTAAACCGGGAACCTTGCGCTACGGTGTGATACAGGATAGACCACTTTGGATTATATTTGCACGTGACATGGTGATTAGGCTGAGTGAAGAGCTTATAATGACCGATAACAAGACATTAGATGCACGTGGTGCCAATGTGCACATCGCTAATGGCGGTCAAATCACTCTACAATTTGTGAAGAACATAATCATCCATAATCTGCACATTCATGATATCAAGGCTGGCAATGGAGGCCTTGTAAGAGACTCGGTGAACCACTATGGATTTCGAACACCGAGTGATGGTGATGGCATCTCGATGTTTGGAGCCTCAAAGGTTTGGATAGATCATGTTTCAATGTCTAATTGTCAAGATGGACTTATTGATGCAGTCATGGCATCTACTGGCATTACCATCTCCAACTGTCACTTCACCCACCATAACGATGTGATACTATTAGGTGCAAGTGATAGCTACTCGAACGATCAGATTCTGCAGGTAACTCTTGCATTTAATCACTTTGGTAAAGGGTTGGTGCAAAGGATGCCGAGATGCCGATGGGGGTTCGTCCATGTAGTCAATAATGACTACACTCACTGGCTTATGTACGCCATTGGCGGCAGCCACAATCCCACCATTGTCAGCCAGGGCAATCGCTTCATCGCCCCACCAGACCCAAATTGCAAAGAGGTGACGAAGAGGGCGTACGCACCAGAAAGCGAGTGGAAATCGTGGAACTGGAGATCAGAGGGAGATCTGATGATGAATGGAGCTTTCTTCGTCCAATCTGGGAGCCCCATCAGGAAATACTCCAAGAAAGATGTTATCCATGCAAAACCTGGGACCTTCGTGAGCAGGCTTACACGATTCTCTGGGCCTTTGAAATGCATAAGGAACAAGCCGTGTTAG

Protein sequence

MRETETTIVVLKLLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVTNNFNQQVHSSFNGANNTRRHLKKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTDNKTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDGDGISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSNDQILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIAPPDPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSGSPIRKYSKKDVIHAKPGTFVSRLTRFSGPLKCIRNKPC

Homology

[Close]

BLAST of Spg036709 vs. NCBI nr
Match: XP_038894610.1 (pectate lyase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 861.3 bits (2224), Expect = 3.6e-246
Identity = 400/439 (91.12%), Postives = 427/439 (97.27%), Query Frame = 0


Query: 1   MRETETTIVVLKLLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVT 60
           MRETETTIV +KLLMI  FVMIFPTL ANIA+FDEVWQKRAEEAKKA++EAYQPHPEEVT
Sbjct: 1   MRETETTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAEEAKKASFEAYQPHPEEVT 60

Query: 61  NNFNQQVHSSFNGANNTRRHLKKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGR 120
           +NFN+QVH S +GANNTRRHL+KYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGR
Sbjct: 61  SNFNKQVHKSLDGANNTRRHLRKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGR 120

Query: 121 GTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTDN 180
           GTTGGK+GR YVV+DSSDNDLLNPKPGTLRYGVIQDRPLWIIFA DMVIRLSEELIMTDN
Sbjct: 121 GTTGGKNGRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDN 180

Query: 181 KTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDGD 240
           KTLD RGANVHIANGGQ+TLQFVKNIIIHNLHIHDIKAGNGG++RDSVNHYGFRTPSDGD
Sbjct: 181 KTLDGRGANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGD 240

Query: 241 GISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSNDQ 300
           GISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFT HNDVILLGASDSYSNDQ
Sbjct: 241 GISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQ 300

Query: 301 ILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIAP 360
           I+QVTLAFNHFGKGLVQRMPRCRWGF+HVVNNDYTHWLMYAIGGSHNPTI+SQGNRFIAP
Sbjct: 301 IMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAP 360

Query: 361 PDPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSGSPIRKYSKKDVIHAKPGTF 420
           P+PN KEVTKRAYA ESEW+SWNWRSEGDLM+NGAFF+QSG+PIR+YSKKDVIH+KPGTF
Sbjct: 361 PNPNSKEVTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRRYSKKDVIHSKPGTF 420

Query: 421 VSRLTRFSGPLKCIRNKPC 440
           V+RLTRF+GPLKCI+N+PC
Sbjct: 421 VTRLTRFAGPLKCIKNQPC 439

[Close]

BLAST of Spg036709 vs. NCBI nr
Match: TYJ99102.1 (pectate lyase-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 826.6 bits (2134), Expect = 9.9e-236
Identity = 383/439 (87.24%), Postives = 417/439 (94.99%), Query Frame = 0


Query: 1   MRETETTIVVLKLLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVT 60
           MRET+TTIV +KLLMI  FVMIFPTL ANIA+FDEVWQKRA EAKKA+ EAYQPHPEEVT
Sbjct: 1   MRETKTTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAIEAKKASLEAYQPHPEEVT 60

Query: 61  NNFNQQVHSSFNGANNTRRHLKKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGR 120
           NNFN+QVH S +GANNTRRHL+KY GPCLATNPIDRCWRCD++WA+NRKKLADCALGFGR
Sbjct: 61  NNFNKQVHRSLDGANNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGR 120

Query: 121 GTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTDN 180
           GTTGGKDG+ YVV+DSSDNDL+NPKPGTLR+ VIQ+RPLWIIFA DMVIRLSEELIMTDN
Sbjct: 121 GTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDN 180

Query: 181 KTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDGD 240
           KTLD RGANVHIANGGQITLQFV+NIIIHNLHIHDIKAGNGG +RDSV+HYG+RT SDGD
Sbjct: 181 KTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGD 240

Query: 241 GISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSNDQ 300
           GISMFG+S+VWIDHVSMSNCQDGLIDAVMAST ITISNCHFTHHNDVILLGASDSYSND+
Sbjct: 241 GISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSNDK 300

Query: 301 ILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIAP 360
           I+QVTLAFNHFGKGLVQRMPRCRWGF+HVVNNDYTHWLMYAIGGSHNPTI+SQGNRFIAP
Sbjct: 301 IMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAP 360

Query: 361 PDPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSGSPIRKYSKKDVIHAKPGTF 420
           P+PN KEVTKR YA E EW+SWNWRSEGDLMMNGAFF+QSG+PIR+YSKKDVIH+KPGTF
Sbjct: 361 PNPNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDVIHSKPGTF 420

Query: 421 VSRLTRFSGPLKCIRNKPC 440
           V+RLTRF+GPLKC  N+PC
Sbjct: 421 VTRLTRFAGPLKCKMNQPC 439

[Close]

BLAST of Spg036709 vs. NCBI nr
Match: XP_004152537.2 (pectate lyase isoform X1 [Cucumis sativus] >KGN64253.1 hypothetical protein Csa_013613 [Cucumis sativus])

HSP 1 Score: 825.9 bits (2132), Expect = 1.7e-235
Identity = 380/439 (86.56%), Postives = 419/439 (95.44%), Query Frame = 0


Query: 1   MRETETTIVVLKLLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVT 60
           MRET+TTIV +KLLMI  FVMIFPTL ANIA+FDEVWQKRA EA+KA++EAY+PHPEE T
Sbjct: 1   MRETKTTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAIEAQKASFEAYEPHPEEET 60

Query: 61  NNFNQQVHSSFNGANNTRRHLKKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGR 120
           NNFN+QVH S +G NNTRRHL+KY GPCLATNPIDRCWRCD++WA+NRKKLADCALGFGR
Sbjct: 61  NNFNKQVHRSLDGGNNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGR 120

Query: 121 GTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTDN 180
            TTGGKDG+ YVV+DSSDNDL+NPKPGTLR+ VIQ+RPLWIIFA DMVIRLSEELI+TD+
Sbjct: 121 RTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIVTDD 180

Query: 181 KTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDGD 240
           KTLD RGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGG++RDSV+HYGFRT SDGD
Sbjct: 181 KTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGMIRDSVSHYGFRTRSDGD 240

Query: 241 GISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSNDQ 300
           GISMFGAS+VWIDHVSMSNCQDGLIDAVMAST ITISNCHFTHHNDVILLGAS+ YSNDQ
Sbjct: 241 GISMFGASRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASNGYSNDQ 300

Query: 301 ILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIAP 360
           I+QVTLAFNHFGKGLVQRMPRCRWGF+HVVNNDYTHWLMYAIGGSHNPTI+SQGNRFIAP
Sbjct: 301 IMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAP 360

Query: 361 PDPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSGSPIRKYSKKDVIHAKPGTF 420
           P+PNCKEVTKR YAPESEW+SWNWRSEGDLMMNGAFF+QSG+PI++YSKKDVIH+KPGTF
Sbjct: 361 PNPNCKEVTKRVYAPESEWRSWNWRSEGDLMMNGAFFIQSGNPIKRYSKKDVIHSKPGTF 420

Query: 421 VSRLTRFSGPLKCIRNKPC 440
           V+RLTRF+GPLKC +N+PC
Sbjct: 421 VTRLTRFAGPLKCKKNQPC 439

[Close]

BLAST of Spg036709 vs. NCBI nr
Match: XP_022994124.1 (pectate lyase-like [Cucurbita maxima])

HSP 1 Score: 817.0 bits (2109), Expect = 7.8e-233
Identity = 382/440 (86.82%), Postives = 413/440 (93.86%), Query Frame = 0


Query: 1   MRETETTIVVLK-LLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEV 60
           MRE E+ IV  K LL+I AF +IFPTL A+IA+FDEVWQ+RA+EAKKAA EAYQPHPEEV
Sbjct: 1   MRELESNIVGAKLLLLIFAFAIIFPTLEADIADFDEVWQQRAQEAKKAALEAYQPHPEEV 60

Query: 61  TNNFNQQVHSSFNGANNTRRHLKKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFG 120
           T NFN+ V+   + ANNTRRHL+KYNGPCLATNPIDRCWRCDK+WAKNRKKLADCALGFG
Sbjct: 61  TENFNEHVNGDLDDANNTRRHLRKYNGPCLATNPIDRCWRCDKNWAKNRKKLADCALGFG 120

Query: 121 RGTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTD 180
           RGTTGGK+GR+YVVQDSSDND+L+PKPGTLRY VIQDRPLWI FA DMVIRL+EELIMTD
Sbjct: 121 RGTTGGKNGRFYVVQDSSDNDMLHPKPGTLRYAVIQDRPLWITFAHDMVIRLAEELIMTD 180

Query: 181 NKTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDG 240
           NKTLD RGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGL+RDSVNHYG RT SDG
Sbjct: 181 NKTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLIRDSVNHYGIRTRSDG 240

Query: 241 DGISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSND 300
           DGIS+FGAS VWIDHVSMSNCQDGLIDAVMAST ITISNCHF HHNDVILLGASDS+S+D
Sbjct: 241 DGISIFGASNVWIDHVSMSNCQDGLIDAVMASTAITISNCHFAHHNDVILLGASDSHSDD 300

Query: 301 QILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIA 360
           QI+QVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGS NPTI+SQGNRFIA
Sbjct: 301 QIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSRNPTIISQGNRFIA 360

Query: 361 PPDPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSGSPIRKYSKKDVIHAKPGT 420
           PPDPNCKEVTKR YAPES+WK+WNWRSEGDL+MNGAFFV+SG+PIRKYSKKDVIHAKPGT
Sbjct: 361 PPDPNCKEVTKRVYAPESDWKTWNWRSEGDLLMNGAFFVESGNPIRKYSKKDVIHAKPGT 420

Query: 421 FVSRLTRFSGPLKCIRNKPC 440
           FV+RLTRF+GPLKC RN+PC
Sbjct: 421 FVTRLTRFAGPLKCTRNQPC 440

[Close]

BLAST of Spg036709 vs. NCBI nr
Match: XP_022954990.1 (pectate lyase-like [Cucurbita moschata])

HSP 1 Score: 812.8 bits (2098), Expect = 1.5e-231
Identity = 380/441 (86.17%), Postives = 411/441 (93.20%), Query Frame = 0


Query: 1   MRETETTIVVLK--LLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEE 60
           MRE ++ I   K  LL+I AF +IFP+L A IA+FDEVWQ+RA+EAKKAA EAYQP PEE
Sbjct: 1   MRELDSNIAGAKLLLLLIFAFAIIFPSLEAGIADFDEVWQQRAQEAKKAALEAYQPRPEE 60

Query: 61  VTNNFNQQVHSSFNGANNTRRHLKKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGF 120
           VT +FN+ V+   + ANNTRRHL+KYNGPCLATNPIDRCWRCDK+W+KNRKKLADCALGF
Sbjct: 61  VTESFNEHVNGDLDDANNTRRHLRKYNGPCLATNPIDRCWRCDKNWSKNRKKLADCALGF 120

Query: 121 GRGTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMT 180
           GRGTTGGK+GR+Y+VQDSSDND+L+PKPGTLRY VIQDRPLWI FA DMVIRL+EELIMT
Sbjct: 121 GRGTTGGKNGRFYIVQDSSDNDMLHPKPGTLRYAVIQDRPLWITFAHDMVIRLAEELIMT 180

Query: 181 DNKTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSD 240
           DNKTLD RGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGL+RDSVNHYG RT SD
Sbjct: 181 DNKTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLIRDSVNHYGIRTRSD 240

Query: 241 GDGISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSN 300
           GDGISMFGAS VWIDHVSMSNCQDGLIDAVMAST ITISNCHF HHNDVILLGASDS+S+
Sbjct: 241 GDGISMFGASNVWIDHVSMSNCQDGLIDAVMASTAITISNCHFAHHNDVILLGASDSHSD 300

Query: 301 DQILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFI 360
           DQI+QVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGS NPTI+SQGNRFI
Sbjct: 301 DQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSRNPTIISQGNRFI 360

Query: 361 APPDPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSGSPIRKYSKKDVIHAKPG 420
           APPDPNCKEVTKR YAPESEWK+WNWRSEGDL+MNGAFFVQSG+PIRKYSKKDVIHAKPG
Sbjct: 361 APPDPNCKEVTKRVYAPESEWKTWNWRSEGDLLMNGAFFVQSGNPIRKYSKKDVIHAKPG 420

Query: 421 TFVSRLTRFSGPLKCIRNKPC 440
           TFV+RLTRF+GPLKCIRNKPC
Sbjct: 421 TFVTRLTRFAGPLKCIRNKPC 441

[Close]

BLAST of Spg036709 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.1e-168
Identity = 276/415 (66.51%), Postives = 331/415 (79.76%), Query Frame = 0


Query: 27  NANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVTNNFNQQVHSSFNGANNTRRHLKKYN- 86
           +ANIA FDE WQK+++ A+  A +AY PHPEEVTN+FN+ VHSSF G N+TRR+L+    
Sbjct: 21  SANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEG-NSTRRNLRTNKL 80

Query: 87  GPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKDGRYYVVQDSSDNDLLNPK 146
           G CLATNPIDRCWRC K+W+ NRK L  C  GFGR TTGG  G  YVV D SD+ L +PK
Sbjct: 81  GQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVTDPSDDSLTDPK 140

Query: 147 PGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTDNKTLDARGANVHIANGGQITLQFVKN 206
            GTLR+GVIQDRPLWIIF + MVIRL +ELI+ ++KT+D RGANV IA G Q+T+QFV N
Sbjct: 141 FGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAGGAQLTVQFVHN 200

Query: 207 IIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDGDGISMFGASKVWIDHVSMSNCQDGLI 266
           +IIH +HIHDIK G GGL+RDS  H G RT SDGDGIS+ G+S +WIDHVS++ C DGLI
Sbjct: 201 VIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHVSLARCSDGLI 260

Query: 267 DAVMASTGITISNCHFTHHNDVILLGASDSYSNDQILQVTLAFNHFGKGLVQRMPRCRWG 326
           D ++ ST ITISNCH T H+DV+LLGASD+Y+ D+I+QVT+AFNHFG+GLVQRMPRCR+G
Sbjct: 261 DVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGLVQRMPRCRYG 320

Query: 327 FVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIAPPDPNCKEVTKRAYAPESEWKSWNWR 386
           FVHVVNNDYTHW+MYA+GGS +PTI+SQGNR+IAP     KEVTKR YA  +EW  W W+
Sbjct: 321 FVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYAEPAEWSKWTWK 380

Query: 387 SEGDLMMNGAFFVQSGSPI-RKYSKKDVIHAKPGTFVSRLTRFSGPLKCIRNKPC 440
           S+GDL ++GAFFV+SG P   KYSKKD+I AKPGTFV RLTRFSG L C  N  C
Sbjct: 381 SQGDLFVSGAFFVESGGPFENKYSKKDLIKAKPGTFVQRLTRFSGALNCKENMEC 434

[Close]

BLAST of Spg036709 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 9.8e-162
Identity = 276/440 (62.73%), Postives = 327/440 (74.32%), Query Frame = 0


Query: 13  LLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVTNNFNQQVHSSFN 72
           L +   F  I P+L A+I ++DEVW++RAEEAK+ A   Y+PHPE VT  FNQ++  +  
Sbjct: 11  LFLCITFATIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTMK 70

Query: 73  GA--------NNTRRHL--KKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGT 132
                     N+TRR L  KKY GPC+ TNPID+CWRCD +WA NRKKLADCA+GFG   
Sbjct: 71  ELKKVKGTHNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSKA 130

Query: 133 TGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTDNKT 192
            GGKDG +YVV D+SD D  +PKPGTLR+ VIQ  PLWIIF R M IRL +E+IM  +KT
Sbjct: 131 IGGKDGEFYVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDKT 190

Query: 193 LDARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDGDGI 252
           +DARG NVHI  G  ITLQ++KN+IIH LHIHDI  GNGG+VRD+V+H G RT SDGDGI
Sbjct: 191 IDARGVNVHITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDGI 250

Query: 253 SMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSNDQIL 312
           S+FGAS +WIDHVSM  C DGLIDAV  STGITISN HFT HN+V+L GASDS S DQ++
Sbjct: 251 SIFGASYIWIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQVM 310

Query: 313 QVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIAPPD 372
           Q+TLAFNHFGK L+QRMPRCRWG++HVVNNDYTHW MYAIGGS +PTI+ QGNRFIAPPD
Sbjct: 311 QITLAFNHFGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPPD 370

Query: 373 PNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSGSPIRKYSKKDV---IHAKPGT 432
              K+VTKR Y PES W  W WRSEG+L MNGA+F +SG P      KD+   I A P  
Sbjct: 371 IFKKQVTKREYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDLYDGISAAPAE 430

Query: 433 FVSRLTRFSGPLKCIRNKPC 440
            V+ +TRF+G L C   KPC
Sbjct: 431 DVTWMTRFAGVLGCKPGKPC 449

[Close]

BLAST of Spg036709 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.0e-150
Identity = 252/442 (57.01%), Postives = 314/442 (71.04%), Query Frame = 0


Query: 17  SAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVTNNFNQQVHSSFNGA-- 76
           S   ++ P +  N+A FD  W +R  +A K    +Y PHP  VTN+FN  V+ + + +  
Sbjct: 18  SFLAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASES 77

Query: 77  -NNTRRHL---------KKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTG 136
            N+TRR L          K +G CLA NPID CWRCD++WA NRKKLADC LGFGR TTG
Sbjct: 78  RNDTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTG 137

Query: 137 GKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTDNKTLD 196
           GKDG  YVV+D+SDNDL+NPKPGTLR+ V +D PLWIIFAR M+I+L +EL++T +KT+D
Sbjct: 138 GKDGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTID 197

Query: 197 ARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDGDGISM 256
            RGA V+I  G  +TLQFV N+IIHN+++  I  GNGGL+RDS  H G RT SDGDGIS+
Sbjct: 198 GRGARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISL 257

Query: 257 FGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSNDQILQV 316
           FGA+ +WIDHVSM+ C DG+IDA+  ST +TISN HFT H +V+L GA D +  D+ +Q+
Sbjct: 258 FGATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQI 317

Query: 317 TLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIAPPDPN 376
           T+AFNHFGK L QRMPRCR+G +HVVNNDYTHW MYAIGG+ NPTI+SQGNRFIAPP+  
Sbjct: 318 TVAFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEE 377

Query: 377 CKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSG-------SPIRKYSKKDVIHAKP 436
            K++TKR Y P  EWKSWNW+SEGD  +NGA+FVQSG        P      K  I  KP
Sbjct: 378 AKQITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKP 437

Query: 437 GTFVSRLTRFSGPLKCIRNKPC 440
           GT V +LT  +G L C   + C
Sbjct: 438 GTMVRKLTMDAGVLGCKLGEAC 459

[Close]

BLAST of Spg036709 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 3.0e-150
Identity = 251/465 (53.98%), Postives = 328/465 (70.54%), Query Frame = 0


Query: 12  KLLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVTNNFNQQVHSSF 71
           KL+      ++ PT+  NI+  DE W +RA+EA++   +AY   P E+ ++F+++ + + 
Sbjct: 8   KLMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNS 67

Query: 72  --------------------------NGANNTRRHLK--------KYNGPCLATNPIDRC 131
                                     +  N+TRR L+        K  GPC A+NPID+C
Sbjct: 68  TDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKC 127

Query: 132 WRCDKHWAKNRKKLADCALGFGRGTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDR 191
           WRC   WAK RKKL  C  GFG  TTGGK GR YVV  + D D++NPKPGTLR+ VIQ  
Sbjct: 128 WRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKE 187

Query: 192 PLWIIFARDMVIRLSEELIMTDNKTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIK 251
           PLWIIF  DM IRL++EL++  +KT+DARGANVH+A+G  IT+QFVKN+IIH LHIH I 
Sbjct: 188 PLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIHHIS 247

Query: 252 AGNGGLVRDSVNHYGFRTPSDGDGISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITIS 311
             +GG++RDSV+H+G RT +DGDG+S++G+S +W+DH+SMS CQDGLIDA++ STGITIS
Sbjct: 248 ESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITIS 307

Query: 312 NCHFTHHNDVILLGASDSYSNDQILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHW 371
           N HFTHHNDV+LLGA ++   D+ +QVT+A+NHFGKGLVQRMPR RWGFVHVVNNDYTHW
Sbjct: 308 NSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHW 367

Query: 372 LMYAIGGSHNPTIVSQGNRFIAPP-DPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAF 431
            +YAIGGS  PTI+S GNRFIAPP  P+ +EVTKR YA E EWK WNWRS+ D+ MNGA+
Sbjct: 368 ELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAY 427

Query: 432 FVQSGSPIRK--YSKKDVIHAKPGTFVSRLTRFSGPLKCIRNKPC 440
           F QSG+P  K  ++++ +I  K G  VS+LT+++G L C   + C
Sbjct: 428 FRQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

[Close]

BLAST of Spg036709 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 1.8e-147
Identity = 256/473 (54.12%), Postives = 326/473 (68.92%), Query Frame = 0


Query: 7   TIVVLKLLMISAFVMIFPTLNANIAN-FDEVWQKRAEEAKKAAYEAYQPHPEEVTNNFNQ 66
           T  + KL+ +     + PT+ AN+A+  DE W  +A EA+K    AY P P E+ ++F++
Sbjct: 3   TARLFKLVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHE 62

Query: 67  Q------------------------VHSSFNGANNTRRHL------------KKYNGPCL 126
           +                        +    +  N+TRR L             K  GPC 
Sbjct: 63  RHYDNSTDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCT 122

Query: 127 ATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKDGRYYVVQDSSDNDLLNPKPGTL 186
           A+NPID+CWRC   WA+ RKKL  C  GFG  TTGGK GR YVV    D+D++NP+PGTL
Sbjct: 123 ASNPIDKCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTL 182

Query: 187 RYGVIQDRPLWIIFARDMVIRLSEELIMTDNKTLDARGANVHIANGGQITLQFVKNIIIH 246
           R+ VIQ  PLWI+F  DM IRLS+EL++T +KT+DARGANVHIA G  IT+Q+V NIIIH
Sbjct: 183 RHAVIQKEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIH 242

Query: 247 NLHIHDIKAGNGGLVRDSVNHYGFRTPSDGDGISMFGASKVWIDHVSMSNCQDGLIDAVM 306
            LH+H I   +GGL+RDS+NH+G R  +DGDGIS+FGA+ +W+DH+SMS CQDGLIDA+M
Sbjct: 243 GLHVHHIVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIM 302

Query: 307 ASTGITISNCHFTHHNDVILLGASDSYSNDQILQVTLAFNHFGKGLVQRMPRCRWGFVHV 366
            ST ITISN HFTHHNDV+LLGA ++  +D+ +QVT+A+NHFGKGLVQRMPR RWGFVHV
Sbjct: 303 GSTAITISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHV 362

Query: 367 VNNDYTHWLMYAIGGSHNPTIVSQGNRFIAPP-DPNCKEVTKRAYAPESEWKSWNWRSEG 426
           VNNDYTHW +YAIGGS  PTI+S GNRFIAPP   + +EVTKR YA ESEWK+WNWRSE 
Sbjct: 363 VNNDYTHWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEK 422

Query: 427 DLMMNGAFFVQSGSPIRK--YSKKDVIHAKPGTFVSRLTRFSGPLKCIRNKPC 440
           D+ MN A+F QSG+P  K  +S++ +I  K G  VS+LT+++G L C   K C
Sbjct: 423 DVFMNNAYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

[Close]

BLAST of Spg036709 vs. ExPASy TrEMBL
Match: A0A5D3BH70 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003070 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 4.8e-236
Identity = 383/439 (87.24%), Postives = 417/439 (94.99%), Query Frame = 0


Query: 1   MRETETTIVVLKLLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVT 60
           MRET+TTIV +KLLMI  FVMIFPTL ANIA+FDEVWQKRA EAKKA+ EAYQPHPEEVT
Sbjct: 1   MRETKTTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAIEAKKASLEAYQPHPEEVT 60

Query: 61  NNFNQQVHSSFNGANNTRRHLKKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGR 120
           NNFN+QVH S +GANNTRRHL+KY GPCLATNPIDRCWRCD++WA+NRKKLADCALGFGR
Sbjct: 61  NNFNKQVHRSLDGANNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGR 120

Query: 121 GTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTDN 180
           GTTGGKDG+ YVV+DSSDNDL+NPKPGTLR+ VIQ+RPLWIIFA DMVIRLSEELIMTDN
Sbjct: 121 GTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDN 180

Query: 181 KTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDGD 240
           KTLD RGANVHIANGGQITLQFV+NIIIHNLHIHDIKAGNGG +RDSV+HYG+RT SDGD
Sbjct: 181 KTLDGRGANVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGD 240

Query: 241 GISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSNDQ 300
           GISMFG+S+VWIDHVSMSNCQDGLIDAVMAST ITISNCHFTHHNDVILLGASDSYSND+
Sbjct: 241 GISMFGSSRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSNDK 300

Query: 301 ILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIAP 360
           I+QVTLAFNHFGKGLVQRMPRCRWGF+HVVNNDYTHWLMYAIGGSHNPTI+SQGNRFIAP
Sbjct: 301 IMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAP 360

Query: 361 PDPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSGSPIRKYSKKDVIHAKPGTF 420
           P+PN KEVTKR YA E EW+SWNWRSEGDLMMNGAFF+QSG+PIR+YSKKDVIH+KPGTF
Sbjct: 361 PNPNSKEVTKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDVIHSKPGTF 420

Query: 421 VSRLTRFSGPLKCIRNKPC 440
           V+RLTRF+GPLKC  N+PC
Sbjct: 421 VTRLTRFAGPLKCKMNQPC 439

[Close]

BLAST of Spg036709 vs. ExPASy TrEMBL
Match: A0A0A0LR32 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G045510 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 8.2e-236
Identity = 380/439 (86.56%), Postives = 419/439 (95.44%), Query Frame = 0


Query: 1   MRETETTIVVLKLLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVT 60
           MRET+TTIV +KLLMI  FVMIFPTL ANIA+FDEVWQKRA EA+KA++EAY+PHPEE T
Sbjct: 1   MRETKTTIVGIKLLMIFGFVMIFPTLKANIADFDEVWQKRAIEAQKASFEAYEPHPEEET 60

Query: 61  NNFNQQVHSSFNGANNTRRHLKKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGR 120
           NNFN+QVH S +G NNTRRHL+KY GPCLATNPIDRCWRCD++WA+NRKKLADCALGFGR
Sbjct: 61  NNFNKQVHRSLDGGNNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGR 120

Query: 121 GTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTDN 180
            TTGGKDG+ YVV+DSSDNDL+NPKPGTLR+ VIQ+RPLWIIFA DMVIRLSEELI+TD+
Sbjct: 121 RTTGGKDGKIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIVTDD 180

Query: 181 KTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDGD 240
           KTLD RGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGG++RDSV+HYGFRT SDGD
Sbjct: 181 KTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGMIRDSVSHYGFRTRSDGD 240

Query: 241 GISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSNDQ 300
           GISMFGAS+VWIDHVSMSNCQDGLIDAVMAST ITISNCHFTHHNDVILLGAS+ YSNDQ
Sbjct: 241 GISMFGASRVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASNGYSNDQ 300

Query: 301 ILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIAP 360
           I+QVTLAFNHFGKGLVQRMPRCRWGF+HVVNNDYTHWLMYAIGGSHNPTI+SQGNRFIAP
Sbjct: 301 IMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAP 360

Query: 361 PDPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSGSPIRKYSKKDVIHAKPGTF 420
           P+PNCKEVTKR YAPESEW+SWNWRSEGDLMMNGAFF+QSG+PI++YSKKDVIH+KPGTF
Sbjct: 361 PNPNCKEVTKRVYAPESEWRSWNWRSEGDLMMNGAFFIQSGNPIKRYSKKDVIHSKPGTF 420

Query: 421 VSRLTRFSGPLKCIRNKPC 440
           V+RLTRF+GPLKC +N+PC
Sbjct: 421 VTRLTRFAGPLKCKKNQPC 439

[Close]

BLAST of Spg036709 vs. ExPASy TrEMBL
Match: A0A6J1K214 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111489953 PE=3 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 3.8e-233
Identity = 382/440 (86.82%), Postives = 413/440 (93.86%), Query Frame = 0


Query: 1   MRETETTIVVLK-LLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEV 60
           MRE E+ IV  K LL+I AF +IFPTL A+IA+FDEVWQ+RA+EAKKAA EAYQPHPEEV
Sbjct: 1   MRELESNIVGAKLLLLIFAFAIIFPTLEADIADFDEVWQQRAQEAKKAALEAYQPHPEEV 60

Query: 61  TNNFNQQVHSSFNGANNTRRHLKKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFG 120
           T NFN+ V+   + ANNTRRHL+KYNGPCLATNPIDRCWRCDK+WAKNRKKLADCALGFG
Sbjct: 61  TENFNEHVNGDLDDANNTRRHLRKYNGPCLATNPIDRCWRCDKNWAKNRKKLADCALGFG 120

Query: 121 RGTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTD 180
           RGTTGGK+GR+YVVQDSSDND+L+PKPGTLRY VIQDRPLWI FA DMVIRL+EELIMTD
Sbjct: 121 RGTTGGKNGRFYVVQDSSDNDMLHPKPGTLRYAVIQDRPLWITFAHDMVIRLAEELIMTD 180

Query: 181 NKTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDG 240
           NKTLD RGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGL+RDSVNHYG RT SDG
Sbjct: 181 NKTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLIRDSVNHYGIRTRSDG 240

Query: 241 DGISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSND 300
           DGIS+FGAS VWIDHVSMSNCQDGLIDAVMAST ITISNCHF HHNDVILLGASDS+S+D
Sbjct: 241 DGISIFGASNVWIDHVSMSNCQDGLIDAVMASTAITISNCHFAHHNDVILLGASDSHSDD 300

Query: 301 QILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIA 360
           QI+QVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGS NPTI+SQGNRFIA
Sbjct: 301 QIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSRNPTIISQGNRFIA 360

Query: 361 PPDPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSGSPIRKYSKKDVIHAKPGT 420
           PPDPNCKEVTKR YAPES+WK+WNWRSEGDL+MNGAFFV+SG+PIRKYSKKDVIHAKPGT
Sbjct: 361 PPDPNCKEVTKRVYAPESDWKTWNWRSEGDLLMNGAFFVESGNPIRKYSKKDVIHAKPGT 420

Query: 421 FVSRLTRFSGPLKCIRNKPC 440
           FV+RLTRF+GPLKC RN+PC
Sbjct: 421 FVTRLTRFAGPLKCTRNQPC 440

[Close]

BLAST of Spg036709 vs. ExPASy TrEMBL
Match: A0A6J1GTX4 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111457080 PE=3 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 7.2e-232
Identity = 380/441 (86.17%), Postives = 411/441 (93.20%), Query Frame = 0


Query: 1   MRETETTIVVLK--LLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEE 60
           MRE ++ I   K  LL+I AF +IFP+L A IA+FDEVWQ+RA+EAKKAA EAYQP PEE
Sbjct: 1   MRELDSNIAGAKLLLLLIFAFAIIFPSLEAGIADFDEVWQQRAQEAKKAALEAYQPRPEE 60

Query: 61  VTNNFNQQVHSSFNGANNTRRHLKKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGF 120
           VT +FN+ V+   + ANNTRRHL+KYNGPCLATNPIDRCWRCDK+W+KNRKKLADCALGF
Sbjct: 61  VTESFNEHVNGDLDDANNTRRHLRKYNGPCLATNPIDRCWRCDKNWSKNRKKLADCALGF 120

Query: 121 GRGTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMT 180
           GRGTTGGK+GR+Y+VQDSSDND+L+PKPGTLRY VIQDRPLWI FA DMVIRL+EELIMT
Sbjct: 121 GRGTTGGKNGRFYIVQDSSDNDMLHPKPGTLRYAVIQDRPLWITFAHDMVIRLAEELIMT 180

Query: 181 DNKTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSD 240
           DNKTLD RGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGL+RDSVNHYG RT SD
Sbjct: 181 DNKTLDGRGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLIRDSVNHYGIRTRSD 240

Query: 241 GDGISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSN 300
           GDGISMFGAS VWIDHVSMSNCQDGLIDAVMAST ITISNCHF HHNDVILLGASDS+S+
Sbjct: 241 GDGISMFGASNVWIDHVSMSNCQDGLIDAVMASTAITISNCHFAHHNDVILLGASDSHSD 300

Query: 301 DQILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFI 360
           DQI+QVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGS NPTI+SQGNRFI
Sbjct: 301 DQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSRNPTIISQGNRFI 360

Query: 361 APPDPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSGSPIRKYSKKDVIHAKPG 420
           APPDPNCKEVTKR YAPESEWK+WNWRSEGDL+MNGAFFVQSG+PIRKYSKKDVIHAKPG
Sbjct: 361 APPDPNCKEVTKRVYAPESEWKTWNWRSEGDLLMNGAFFVQSGNPIRKYSKKDVIHAKPG 420

Query: 421 TFVSRLTRFSGPLKCIRNKPC 440
           TFV+RLTRF+GPLKCIRNKPC
Sbjct: 421 TFVTRLTRFAGPLKCIRNKPC 441

[Close]

BLAST of Spg036709 vs. ExPASy TrEMBL
Match: A0A6J1C763 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009024 PE=3 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 3.7e-228
Identity = 370/409 (90.46%), Postives = 394/409 (96.33%), Query Frame = 0


Query: 32  NFDEVWQKRAEEAKKAAYEAYQPHPEEVTNNFNQQVHSSFNGANNTRRHLKKYN-GPCLA 91
           NFDEVWQKRA+EAKKAA EAYQPHPEEVT+NFNQQVH S +GANNTRRHLK Y+ GPCLA
Sbjct: 50  NFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSLDGANNTRRHLKHYSGGPCLA 109

Query: 92  TNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKDGRYYVVQDSSDNDLLNPKPGTLR 151
           TNPIDRCWRCDKHWAKNRKKLA+CALGFGRGTTGGKDGRYYVV+DSSDNDLLNPKPGTLR
Sbjct: 110 TNPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRYYVVRDSSDNDLLNPKPGTLR 169

Query: 152 YGVIQDRPLWIIFARDMVIRLSEELIMTDNKTLDARGANVHIANGGQITLQFVKNIIIHN 211
           YGVIQ++PLWI+FA DMVIRLSEELI+TDNKT+D RGANVHIANGGQITLQFVKNIII N
Sbjct: 170 YGVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANVHIANGGQITLQFVKNIIISN 229

Query: 212 LHIHDIKAGNGGLVRDSVNHYGFRTPSDGDGISMFGASKVWIDHVSMSNCQDGLIDAVMA 271
           LHIHDIK G+GGL+RDSV+HYG RTPSDGDGISMFGA+K+WIDHVSMSNCQDGLIDAVMA
Sbjct: 230 LHIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKIWIDHVSMSNCQDGLIDAVMA 289

Query: 272 STGITISNCHFTHHNDVILLGASDSYSNDQILQVTLAFNHFGKGLVQRMPRCRWGFVHVV 331
           STGITISNCHFT HNDV+LLGASDSYSNDQI+QVTLAFNHFGKGLVQRMPRCRWGFVHVV
Sbjct: 290 STGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFVHVV 349

Query: 332 NNDYTHWLMYAIGGSHNPTIVSQGNRFIAPPDPNCKEVTKRAYAPESEWKSWNWRSEGDL 391
           NNDYTHWLMYAIGGSHNPTI+SQGNRFIAPPDPNCKEVTKRAYA ESEWKSWNWRSEGDL
Sbjct: 350 NNDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTKRAYASESEWKSWNWRSEGDL 409

Query: 392 MMNGAFFVQSGSPIRKYSKKDVIHAKPGTFVSRLTRFSGPLKCIRNKPC 440
           MMNGAFF++SGSPIRKYSKKDVI AKPG+FV+RLTRFSGPLKC  N+PC
Sbjct: 410 MMNGAFFIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGPLKCRNNRPC 458

[Close]

BLAST of Spg036709 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 534.6 bits (1376), Expect = 7.2e-152
Identity = 252/442 (57.01%), Postives = 314/442 (71.04%), Query Frame = 0


Query: 17  SAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVTNNFNQQVHSSFNGA-- 76
           S   ++ P +  N+A FD  W +R  +A K    +Y PHP  VTN+FN  V+ + + +  
Sbjct: 18  SFLAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASES 77

Query: 77  -NNTRRHL---------KKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTG 136
            N+TRR L          K +G CLA NPID CWRCD++WA NRKKLADC LGFGR TTG
Sbjct: 78  RNDTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTG 137

Query: 137 GKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTDNKTLD 196
           GKDG  YVV+D+SDNDL+NPKPGTLR+ V +D PLWIIFAR M+I+L +EL++T +KT+D
Sbjct: 138 GKDGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTID 197

Query: 197 ARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDGDGISM 256
            RGA V+I  G  +TLQFV N+IIHN+++  I  GNGGL+RDS  H G RT SDGDGIS+
Sbjct: 198 GRGARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISL 257

Query: 257 FGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSNDQILQV 316
           FGA+ +WIDHVSM+ C DG+IDA+  ST +TISN HFT H +V+L GA D +  D+ +Q+
Sbjct: 258 FGATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQI 317

Query: 317 TLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIAPPDPN 376
           T+AFNHFGK L QRMPRCR+G +HVVNNDYTHW MYAIGG+ NPTI+SQGNRFIAPP+  
Sbjct: 318 TVAFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEE 377

Query: 377 CKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSG-------SPIRKYSKKDVIHAKP 436
            K++TKR Y P  EWKSWNW+SEGD  +NGA+FVQSG        P      K  I  KP
Sbjct: 378 AKQITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKP 437

Query: 437 GTFVSRLTRFSGPLKCIRNKPC 440
           GT V +LT  +G L C   + C
Sbjct: 438 GTMVRKLTMDAGVLGCKLGEAC 459

[Close]

BLAST of Spg036709 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 532.7 bits (1371), Expect = 2.8e-151
Identity = 250/465 (53.76%), Postives = 328/465 (70.54%), Query Frame = 0


Query: 12  KLLMISAFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVTNNFNQQVHSSF 71
           KL+      ++ PT+  NI+  DE W +RA+EA++   +AY   P E+ ++F+++ + + 
Sbjct: 8   KLMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNS 67

Query: 72  --------------------------NGANNTRRHLK--------KYNGPCLATNPIDRC 131
                                     +  N+TRR L+        K  GPC A+NPID+C
Sbjct: 68  TDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKC 127

Query: 132 WRCDKHWAKNRKKLADCALGFGRGTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDR 191
           WRC   WAK RKKL  C  GFG  TTGGK GR YVV  + D D++NPKPGTLR+ VIQ  
Sbjct: 128 WRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKE 187

Query: 192 PLWIIFARDMVIRLSEELIMTDNKTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIK 251
           PLWIIF  DM IRL++EL++  +KT+DARGANVH+A+G  IT+QFVKN++IH LHIH I 
Sbjct: 188 PLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIHHIS 247

Query: 252 AGNGGLVRDSVNHYGFRTPSDGDGISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITIS 311
             +GG++RDSV+H+G RT +DGDG+S++G+S +W+DH+SMS CQDGLIDA++ STGITIS
Sbjct: 248 ESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITIS 307

Query: 312 NCHFTHHNDVILLGASDSYSNDQILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHW 371
           N HFTHHNDV+LLGA ++   D+ +QVT+A+NHFGKGLVQRMPR RWGFVHVVNNDYTHW
Sbjct: 308 NSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHW 367

Query: 372 LMYAIGGSHNPTIVSQGNRFIAPP-DPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAF 431
            +YAIGGS  PTI+S GNRFIAPP  P+ +EVTKR YA E EWK WNWRS+ D+ MNGA+
Sbjct: 368 ELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAY 427

Query: 432 FVQSGSPIRK--YSKKDVIHAKPGTFVSRLTRFSGPLKCIRNKPC 440
           F QSG+P  K  ++++ +I  K G  VS+LT+++G L C   + C
Sbjct: 428 FRQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

[Close]

BLAST of Spg036709 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 523.9 bits (1348), Expect = 1.3e-148
Identity = 256/473 (54.12%), Postives = 326/473 (68.92%), Query Frame = 0


Query: 7   TIVVLKLLMISAFVMIFPTLNANIAN-FDEVWQKRAEEAKKAAYEAYQPHPEEVTNNFNQ 66
           T  + KL+ +     + PT+ AN+A+  DE W  +A EA+K    AY P P E+ ++F++
Sbjct: 3   TARLFKLVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHE 62

Query: 67  Q------------------------VHSSFNGANNTRRHL------------KKYNGPCL 126
           +                        +    +  N+TRR L             K  GPC 
Sbjct: 63  RHYDNSTDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCT 122

Query: 127 ATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKDGRYYVVQDSSDNDLLNPKPGTL 186
           A+NPID+CWRC   WA+ RKKL  C  GFG  TTGGK GR YVV    D+D++NP+PGTL
Sbjct: 123 ASNPIDKCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTL 182

Query: 187 RYGVIQDRPLWIIFARDMVIRLSEELIMTDNKTLDARGANVHIANGGQITLQFVKNIIIH 246
           R+ VIQ  PLWI+F  DM IRLS+EL++T +KT+DARGANVHIA G  IT+Q+V NIIIH
Sbjct: 183 RHAVIQKEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIH 242

Query: 247 NLHIHDIKAGNGGLVRDSVNHYGFRTPSDGDGISMFGASKVWIDHVSMSNCQDGLIDAVM 306
            LH+H I   +GGL+RDS+NH+G R  +DGDGIS+FGA+ +W+DH+SMS CQDGLIDA+M
Sbjct: 243 GLHVHHIVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIM 302

Query: 307 ASTGITISNCHFTHHNDVILLGASDSYSNDQILQVTLAFNHFGKGLVQRMPRCRWGFVHV 366
            ST ITISN HFTHHNDV+LLGA ++  +D+ +QVT+A+NHFGKGLVQRMPR RWGFVHV
Sbjct: 303 GSTAITISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHV 362

Query: 367 VNNDYTHWLMYAIGGSHNPTIVSQGNRFIAPP-DPNCKEVTKRAYAPESEWKSWNWRSEG 426
           VNNDYTHW +YAIGGS  PTI+S GNRFIAPP   + +EVTKR YA ESEWK+WNWRSE 
Sbjct: 363 VNNDYTHWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEK 422

Query: 427 DLMMNGAFFVQSGSPIRK--YSKKDVIHAKPGTFVSRLTRFSGPLKCIRNKPC 440
           D+ MN A+F QSG+P  K  +S++ +I  K G  VS+LT+++G L C   K C
Sbjct: 423 DVFMNNAYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

[Close]

BLAST of Spg036709 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 511.5 bits (1316), Expect = 6.6e-145
Identity = 246/441 (55.78%), Postives = 313/441 (70.98%), Query Frame = 0


Query: 18  AFVMIFPTLNANIANFDEVWQKRAEEAKKAAYEAYQPHPEEVTNNFNQQVHSSFNG---A 77
           +  ++ P++ A+IA +DE W +R   A +   E+Y P+PE VT++FN     +       
Sbjct: 15  SLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAALAMETTGIV 74

Query: 78  NNTRRHLK---------KYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGG 137
           N TRR L+         +  G   + N ID+CWR DK+W KNRKKLADC LGFGR TTGG
Sbjct: 75  NETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVLGFGRKTTGG 134

Query: 138 KDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEELIMTDNKTLDA 197
           K+G  YVV D SDNDLL PKPGT+R+ V +DRPLWIIFAR M+I+L +ELI+T++KT+D 
Sbjct: 135 KNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELIITNDKTIDG 194

Query: 198 RGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRTPSDGDGISMF 257
           RGA ++I  G  +TLQFV+N+IIHN+HI  IK G GGL+ DS  H+G RT SDGDGI++F
Sbjct: 195 RGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTVSDGDGINIF 254

Query: 258 GASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDSYSNDQILQVT 317
           GA+ VWIDHVSM++C DG+IDA+M ST ITISN HFT H++V+L G ++    D+ +Q+T
Sbjct: 255 GATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKDVIDKKMQIT 314

Query: 318 LAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGNRFIAPPDPNC 377
           +AFNHFGK L QRMPR R+G VHVVNNDYTHW MYAIGG+ NPTI+SQGNRFIAPP  + 
Sbjct: 315 VAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPIEDS 374

Query: 378 KEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSG-------SPIRKYSKKDVIHAKPG 437
           K+VTKR Y P  EWKSWNW+SE D  +NGA+FVQSG       +P     +K  I  +PG
Sbjct: 375 KQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKFAIRPQPG 434

Query: 438 TFVSRLTRFSGPLKCIRNKPC 440
           T V RLT+ +G L C   K C
Sbjct: 435 TKVRRLTKDAGTLGCKPGKSC 455

[Close]

BLAST of Spg036709 vs. TAIR 10
Match: AT5G63180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 494.6 bits (1272), Expect = 8.3e-140
Identity = 235/387 (60.72%), Postives = 282/387 (72.87%), Query Frame = 0


Query: 56  PEEVTNNFNQQVHSSFNGANNTRRHLKKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCA 115
           PE V    +++++ S      +RR L  ++  C + NPID CWRCDK W KNRK+LADC 
Sbjct: 53  PELVVEEVHRKINESI-----SRRKLGFFS--CGSGNPIDDCWRCDKDWEKNRKRLADCG 112

Query: 116 LGFGRGTTGGKDGRYYVVQDSSDNDLLNPKPGTLRYGVIQDRPLWIIFARDMVIRLSEEL 175
           +GFG+   GG+DG  YVV D  ++D +NP+PGTLRY VIQD PLWIIF RDM I+L EEL
Sbjct: 113 IGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQDEPLWIIFKRDMTIQLKEEL 172

Query: 176 IMTDNKTLDARGANVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGLVRDSVNHYGFRT 235
           IM   KTLD RGA+VHI+ G  IT+Q+V NIIIH LHIHD K G    VRDS  HYG+RT
Sbjct: 173 IMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDCKQGGNTYVRDSPEHYGYRT 232

Query: 236 PSDGDGISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTHHNDVILLGASDS 295
            SDGDG+S+FG S VW+DH S+SNC DGLIDA+  ST ITISN + THHN V+LLG SD+
Sbjct: 233 VSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITISNNYLTHHNKVMLLGHSDT 292

Query: 296 YSNDQILQVTLAFNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIVSQGN 355
           Y  D+ +QVT+AFNHFG+GLVQRMPRCR G+ HVVNNDYTHW MYAIGGS NPTI SQGN
Sbjct: 293 YEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGN 352

Query: 356 RFIAPPDPNCKEVTKRAYAPESEWKSWNWRSEGDLMMNGAFFVQSGSPIRK---YSKKDV 415
           RF+AP D + KEVTK   APE EW++WNWRSEGDL++NGAFF  SG+   K   YSK   
Sbjct: 353 RFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAFFTYSGAGPAKSSSYSKASS 412

Query: 416 IHAKPGTFVSRLTRFSGPLKCIRNKPC 440
           + A+P + V  +T  SG L C R   C
Sbjct: 413 LAARPSSHVGEITIASGALSCKRGSHC 432

The following BLAST results are available for this feature:

BLAST of Spg036709 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038894610.1	3.6e-246	91.12	pectate lyase-like isoform X2 [Benincasa hispida]	[more]
TYJ99102.1	9.9e-236	87.24	pectate lyase-like isoform X2 [Cucumis melo var. makuwa]	[more]
XP_004152537.2	1.7e-235	86.56	pectate lyase isoform X1 [Cucumis sativus] >KGN64253.1 hypothetical protein Csa_...	[more]
XP_022994124.1	7.8e-233	86.82	pectate lyase-like [Cucurbita maxima]	[more]
XP_022954990.1	1.5e-231	86.17	pectate lyase-like [Cucurbita moschata]	[more]

BLAST of Spg036709 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P40973	1.1e-168	66.51	Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1	[more]
P15722	9.8e-162	62.73	Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1	[more]
Q9M9S2	1.0e-150	57.01	Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1	[more]
Q9LFP5	3.0e-150	53.98	Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1	[more]
Q9SRH4	1.8e-147	54.12	Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1	[more]

BLAST of Spg036709 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BH70	4.8e-236	87.24	Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003070...	[more]
A0A0A0LR32	8.2e-236	86.56	Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G045510 PE=3 SV=1	[more]
A0A6J1K214	3.8e-233	86.82	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111489953 PE=3 SV=1	[more]
A0A6J1GTX4	7.2e-232	86.17	Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111457080 PE=3 SV=1	[more]
A0A6J1C763	3.7e-228	90.46	Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009024 PE=3 SV=1	[more]

BLAST of Spg036709 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14420.1	7.2e-152	57.01	Pectate lyase family protein	[more]
AT5G15110.1	2.8e-151	53.76	Pectate lyase family protein	[more]
AT3G01270.1	1.3e-148	54.12	Pectate lyase family protein	[more]
AT2G02720.1	6.6e-145	55.78	Pectate lyase family protein	[more]
AT5G63180.1	8.3e-140	60.72	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 114..131 score: 62.96 coord: 174..190 score: 56.86 coord: 138..163 score: 69.41 coord: 382..406 score: 53.52 coord: 318..337 score: 76.43 coord: 238..259 score: 59.74 coord: 340..359 score: 78.81
IPR002022	Pectate lyase	SMART	SM00656	amb_all	coord: 166..363 e-value: 1.9E-98 score: 343.0
IPR002022	Pectate lyase	PFAM	PF00544	Pectate_lyase_4	coord: 180..357 e-value: 3.2E-19 score: 69.4
IPR007524	Pectate lyase, N-terminal	PFAM	PF04431	Pec_lyase_N	coord: 29..81 e-value: 1.4E-22 score: 79.7
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 91..432 e-value: 3.7E-114 score: 383.3
None	No IPR available	PANTHER	PTHR31683:SF146	PECTATE LYASE	coord: 10..439
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 10..439
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 92..429

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg036709.1	Spg036709.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030570	pectate lyase activity