Spg036685 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg036685
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Cytochrome P450
Location	scaffold5: 45958805 .. 45961999 (-)
RNA-Seq Expression	Spg036685
Synteny	Spg036685

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGAAACATCCAATCCTTTCCTTTCGGCGTCGTCGCTCGGGCTTGTCTTCTGATTGGAAAATTATATCGAAGATACGATGTGGACGATCGTGCTCGGTTTGGCGACGCTGGCAATCGCCTATTACATCCATTGGATCAACAAATGGAGGGATTCAAAATTCAACGGAGTTCTGCCGCCAGGCACCATGGGCCTGCCCCTCATCGGAGAGACCATTCAGTTGAGTCGACCCAGTGACTCCCTTGATGTTCACCCTTTCATTCAGAGCAAAGTTAAAAGGTATTTTTAATTATTATTGTTATAAACATTATAAACACTCGTAACCGCTTTTAAAATAATTGATGGCAGTTATGTTTCAAGTAACTAAGTGTTAGAAATATGATAAAATGAGAATTCAAAAAAGTTTGTTAGGAGTGGCATCAGAATTAGTTTTATCAAATGCTAATAACAGTTAAAATTCTCTCGCAATCTCGCTTTTAATGTATGCAAAATCAATCCTGAATTAGGTCATATAATCAATTCAAACGTTAAACTACGAGTCAAATCGAAAATTTTTGAACAACAATAATAGACGAAGAACTAAAACATATATTTAGCGGAATTAATTAAATTAAAATTTAAATTGGGGGAAATGTGATGGTTTTTAGATATGGACCAATCTTCAAGACATGTTTGGCGGGAAGGCCGGTGGTGGTTTCAACAGATGCAGAGTTCAACCATTACATAATGTTGCAAGAAGGAAGGGCTGTGGAAATGTGGTATTTGGATACACTCTCCAAATTCTTTGGCCTTGACACTGAGTGGCTCAAAGCCCTTGGCCTCATCCACAAGTACATCAGAAGCATTACTTTGAACCACTTCGGCGCCGAGTCGCTCCGCGAGCGCTTTCTTCCTCGTATCGAAGAATCCGCTAGGGAAACCCTTCATTATTGGTCCACTCAATCCAGCGTTGAAGTCAAAGAATCTGCTGCTGCGGTATGTCAATTTCCTTAACCTAACCAATATCTGATGTGAGATCCCCAACATGTTCCTAAAAGATAGATCGAGTTTAGTACTAGTTTAAGAATAATTCTCTTTGTTGAATTTTGCAGATGGTCTTCAGAACTTCGGTGGCTAAGATGTTTAGTGAAGATTCGAGTAAATTACTGACAGGCGGTCTCACCAAGAAGTTCACCGGACTTCTCGGAGGTTTTCTCACTTTGCCTCTAAATTTGCCTGGCACCACTTACAACAAATGCATGAAGGTGATTATCAGAGCTATCGCTCGATCATAAAGCTTAAACTAGTGGATTATGTTCATTATGCCAAGAACGAAATATTCAATTTTTAAAATATTTTTTTTTAATTTTTGGATGACTCTAGGACATGAAGGAAATCCAAAAGAAGCTGAAAGATATTTTAGAGGACAGGTTGGCTTCAGGGGCAGGTAGTGATGAGGATTTCTTGGGGCAGGCCATTAAAGATAAGGAATCACAAAAGTTCATCACAGAGGAATTCATCATCCAATTGTTGTTTTCCATCAGCTTTGCTAGCTTTGAGTCCATCTCCACCACTCTCACTTTGATTCTCAACTTCCTCGCCGGTCATCCGACGTAGTGAAAGAGTTGGAAGTAAGACCCGAAAGACACTTAAAAAAAAATTATTCGATGAGGCTGGAACAATGTGACTGTTTCATACGTCGTTGGATGTTAAAGGAGAAATTAAAAATCGTGCTTTAATTTTACATCCAACGCTTAAGGGACAACCACGTCGTAACAGCCACGTTAAATAGTTTTTTTTTTCACTAATGAATATTTACTAAGTTTTGTGGATGATTTTAATGTATATTTTGTTGTGTGTTAAAGGCTGAGCATGAAGCCATCCGAAAAGCTAGGGCAGATCCAGATGGGCCAATCACTTGGGAAGAATACAAATCCATGACTTTCACACTTAATGTAATATTTAAATCTTTTATATATACAATTTGTCAATTTCTTGTTCATTTCTTTTTCCAATTTGATAACTTTTTTTTTTCTTTTGTCTATTTTGGATTCTAGGTTATCAGTGAAGCACTTAGGTTGGGGAGTGTAACACCTGCCTTGTTGAGGAAAACAACCAAAGAAATTCAAATAAAAGGTATAAAAAATAATTAATTTTATGGTATTTTGTTAAATACGGTTGTTATCGTATCAATTAATTGAGATGCTATATTCATAGTAATTAACTGATTCACTCATTTGCATTTGATTAATATAGGATACACGATCCCAGAAGGATGGACGGTTATGCTTGTCACTGCTTCTCGTCATAGAGACCCAGCAGTCTATAAGGATCCAGATACCTTCAATCCATGGCGTTGGAAGGTACACCCTAAAAATAAAAAGAAAGACAAAAAAAAAAAAAAAAAACCATAATAAATACATTTTCTAATATACTTCTAATTAATTAATTGTTAACACAATGCTAACTTTGTTTTAAATCATTATGGATATATCATAGGACTTGGACTCAATTACTGTTCAGAAGAACTTCATGCCATTTGGGGGAGGCTTAAGGCATTGTGCTGGTGCTGAGTACTCTAAAGTCTACTTGAGCACCTTCCTCCATATCCTTTTCACCAAATACAGGTGCGTAGTCTATAATTTCAGGTTTCGTTTGATAACGATTTTGTTTTAATTTTTTGTTTCTATTATTTCTTTACCGTGATTTTCACCTTTTTTTAGGAGCATTAAAATTCTCAGCCAAATTCCAAAAATAAAGATAAAATTTTAAAGGCTATTGTTTTAGTTTTCAAATTTTAGTTTGGATTTTTAAAACATTTTTAAAAAATAGATAGAAAATTGTTATGAAATGGGGTTAAGTTTTCAAATTTATTAGTAATTTAACATTAGTATATAAGATAGTTTAATTGTGTATATATACAATCTAATTTAATTTAAATTTTGATAGATGGACCAAACTCAAGGGAGGAAAGATTGCAAGAGCTCATATATTGAAGTTTGAAGATGGGCTACATGTGAAGTTCACTCCCAAGGAATGATTGAATAAATTCAAAAAAAAAAAATGTCATTTCTATTATTCTTATGGGATCACCTACTTGTACTACTATTTTCTTCTTCCTTTGCTTCAATAAGAGATAATGGTCTTCTAAATCTCCTTGTATTAAAAGTATTTTGTAATTAAGTTTAATTAATTAGAACATGGTTTCATTAATGGCC

mRNA sequence

ATGTGGACGATCGTGCTCGGTTTGGCGACGCTGGCAATCGCCTATTACATCCATTGGATCAACAAATGGAGGGATTCAAAATTCAACGGAGTTCTGCCGCCAGGCACCATGGGCCTGCCCCTCATCGGAGAGACCATTCAGTTGAGTCGACCCAGTGACTCCCTTGATGTTCACCCTTTCATTCAGAGCAAAGTTAAAAGATATGGACCAATCTTCAAGACATGTTTGGCGGGAAGGCCGGTGGTGGTTTCAACAGATGCAGAGTTCAACCATTACATAATGTTGCAAGAAGGAAGGGCTGTGGAAATGTGGTATTTGGATACACTCTCCAAATTCTTTGGCCTTGACACTGAGTGGCTCAAAGCCCTTGGCCTCATCCACAAGTACATCAGAAGCATTACTTTGAACCACTTCGGCGCCGAGTCGCTCCGCGAGCGCTTTCTTCCTCGTATCGAAGAATCCGCTAGGGAAACCCTTCATTATTGGTCCACTCAATCCAGCGTTGAAGTCAAAGAATCTGCTGCTGCGATGGTCTTCAGAACTTCGGTGGCTAAGATGTTTAGTGAAGATTCGAGTAAATTACTGACAGGCGGTCTCACCAAGAAGTTCACCGGACTTCTCGGAGGTTTTCTCACTTTGCCTCTAAATTTGCCTGGCACCACTTACAACAAATGCATGAAGGCTGAGCATGAAGCCATCCGAAAAGCTAGGGCAGATCCAGATGGGCCAATCACTTGGGAAGAATACAAATCCATGACTTTCACACTTAATGTTATCAGTGAAGCACTTAGGTTGGGGAGTGTAACACCTGCCTTGTTGAGGAAAACAACCAAAGAAATTCAAATAAAAGGATACACGATCCCAGAAGGATGGACGGTTATGCTTGTCACTGCTTCTCGTCATAGAGACCCAGCAGTCTATAAGGATCCAGATACCTTCAATCCATGGCGTTGGAAGGACTTGGACTCAATTACTGTTCAGAAGAACTTCATGCCATTTGGGGGAGGCTTAAGGCATTGTGCTGGTGCTGAGTACTCTAAAGTCTACTTGAGCACCTTCCTCCATATCCTTTTCACCAAATACAGGTGCGTAGTCTATAATTTCAGATGGACCAAACTCAAGGGAGGAAAGATTGCAAGAGCTCATATATTGAAGTTTGAAGATGGGCTACATGTGAAGTTCACTCCCAAGGAATGA

Coding sequence (CDS)

ATGTGGACGATCGTGCTCGGTTTGGCGACGCTGGCAATCGCCTATTACATCCATTGGATCAACAAATGGAGGGATTCAAAATTCAACGGAGTTCTGCCGCCAGGCACCATGGGCCTGCCCCTCATCGGAGAGACCATTCAGTTGAGTCGACCCAGTGACTCCCTTGATGTTCACCCTTTCATTCAGAGCAAAGTTAAAAGATATGGACCAATCTTCAAGACATGTTTGGCGGGAAGGCCGGTGGTGGTTTCAACAGATGCAGAGTTCAACCATTACATAATGTTGCAAGAAGGAAGGGCTGTGGAAATGTGGTATTTGGATACACTCTCCAAATTCTTTGGCCTTGACACTGAGTGGCTCAAAGCCCTTGGCCTCATCCACAAGTACATCAGAAGCATTACTTTGAACCACTTCGGCGCCGAGTCGCTCCGCGAGCGCTTTCTTCCTCGTATCGAAGAATCCGCTAGGGAAACCCTTCATTATTGGTCCACTCAATCCAGCGTTGAAGTCAAAGAATCTGCTGCTGCGATGGTCTTCAGAACTTCGGTGGCTAAGATGTTTAGTGAAGATTCGAGTAAATTACTGACAGGCGGTCTCACCAAGAAGTTCACCGGACTTCTCGGAGGTTTTCTCACTTTGCCTCTAAATTTGCCTGGCACCACTTACAACAAATGCATGAAGGCTGAGCATGAAGCCATCCGAAAAGCTAGGGCAGATCCAGATGGGCCAATCACTTGGGAAGAATACAAATCCATGACTTTCACACTTAATGTTATCAGTGAAGCACTTAGGTTGGGGAGTGTAACACCTGCCTTGTTGAGGAAAACAACCAAAGAAATTCAAATAAAAGGATACACGATCCCAGAAGGATGGACGGTTATGCTTGTCACTGCTTCTCGTCATAGAGACCCAGCAGTCTATAAGGATCCAGATACCTTCAATCCATGGCGTTGGAAGGACTTGGACTCAATTACTGTTCAGAAGAACTTCATGCCATTTGGGGGAGGCTTAAGGCATTGTGCTGGTGCTGAGTACTCTAAAGTCTACTTGAGCACCTTCCTCCATATCCTTTTCACCAAATACAGGTGCGTAGTCTATAATTTCAGATGGACCAAACTCAAGGGAGGAAAGATTGCAAGAGCTCATATATTGAAGTTTGAAGATGGGCTACATGTGAAGTTCACTCCCAAGGAATGA

Protein sequence

MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPFIQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWLKALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFRTSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMKAEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTTKEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGLRHCAGAEYSKVYLSTFLHILFTKYRCVVYNFRWTKLKGGKIARAHILKFEDGLHVKFTPKE

Homology

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BLAST of Spg036685 vs. NCBI nr
Match: XP_038895732.1 (cucurbitadienol 11-hydroxylase [Benincasa hispida])

HSP 1 Score: 738.0 bits (1904), Expect = 4.2e-209
Identity = 371/481 (77.13%), Postives = 381/481 (79.21%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTI+LGLAT+A+ YYIHW+NKW+DSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTILLGLATVAVVYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAESLR+RFLPRIEESARETLHYWSTQ SVEVKESAAAMVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAESLRDRFLPRIEESARETLHYWSTQPSVEVKESAAAMVFR 180

Query: 181 TSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMK------------- 240
           TS+ KMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMK             
Sbjct: 181 TSIVKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMKDMKEIQKKLKDIL 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 EERLAKGASSEEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300

Query: 301 ----------AEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTT 360
                     AEHEAIRKARADPDGPITWEEYKSMTFTLNVISE LRLGSVTPALLRKTT
Sbjct: 301 DHPEVVKELEAEHEAIRKARADPDGPITWEEYKSMTFTLNVISETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGL 399
           KEIQIKGY IPEGWTVMLVTASRHRDP VYKDP TFNPWRWK++DS+TVQKNFMPFGGGL
Sbjct: 361 KEIQIKGYIIPEGWTVMLVTASRHRDPEVYKDPATFNPWRWKEIDSMTVQKNFMPFGGGL 420

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BLAST of Spg036685 vs. NCBI nr
Match: NP_001289636.1 (Cytochrome P450 87A3-like [Cucumis sativus] >AIT72037.1 cytochrome P450 [Cucumis sativus] >KGN64240.1 hypothetical protein Csa_013216 [Cucumis sativus])

HSP 1 Score: 733.8 bits (1893), Expect = 7.9e-208
Identity = 370/481 (76.92%), Postives = 381/481 (79.21%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTI+LGLATLAIAYYIHW+NKW+DSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQ KVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQ+SVEVKESAAAMVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180

Query: 181 TSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMK------------- 240
           TS+ KMFSEDSSKLLT GLTKKFTGLLGGFLTLPLNLPGTTY+KC+K             
Sbjct: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300

Query: 301 ----------AEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTT 360
                     AEHEAIRKARADPDGPITWEEYKSM FTLNVI E LRLGSVTPALLRKTT
Sbjct: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGL 399
           KEIQIKGYTIPEGWTVMLVTASRHRDP VYKDPDTFNPWRWK+LDSIT+QKNFMPFGGGL
Sbjct: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420

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BLAST of Spg036685 vs. NCBI nr
Match: XP_008437370.1 (PREDICTED: cytochrome P450 87A3-like [Cucumis melo] >TYJ99092.1 cytochrome P450 87A3-like [Cucumis melo var. makuwa])

HSP 1 Score: 733.4 bits (1892), Expect = 1.0e-207
Identity = 369/481 (76.72%), Postives = 381/481 (79.21%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTI+LGLATLAIAYYIHW+NKW+DSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQ SVEVKESAAAMVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQPSVEVKESAAAMVFR 180

Query: 181 TSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMK------------- 240
           TS+ KMFSEDSSKLLT GLTKKFTGLLGGFLTLPLN+PGTTY+KC+K             
Sbjct: 181 TSIVKMFSEDSSKLLTAGLTKKFTGLLGGFLTLPLNVPGTTYHKCIKDMKEIQKKLKDIL 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 EERLAKGVSIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300

Query: 301 ----------AEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTT 360
                     AEHEAIRKARADPDGPITWEEYKSM FTLNVI E LRLGSVTPALLRKTT
Sbjct: 301 DHPDVAKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGL 399
           KEIQIKGYTIPEGWTVMLVTASRHRDP VYKDPDTFNPWRWK+LDSIT+Q+NFMPFGGGL
Sbjct: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQRNFMPFGGGL 420

[Close]

BLAST of Spg036685 vs. NCBI nr
Match: KAG6573008.1 (Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 716.1 bits (1847), Expect = 1.7e-202
Identity = 357/481 (74.22%), Postives = 376/481 (78.17%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MW IV+GLATLA+AYYIHWINKW+DSKFNGVLPPGTMGLPL+GET+QL+RPSDSLDVHPF
Sbjct: 1   MWAIVVGLATLAVAYYIHWINKWKDSKFNGVLPPGTMGLPLVGETLQLARPSDSLDVHPF 60

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           I+ KVKRYGPIFKTCLAGRPVVVSTDAEFN+YIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IKKKVKRYGPIFKTCLAGRPVVVSTDAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           KALG IHKYIRSITLNHFGAESLRERFLPRIEESA+ETL YW+TQ SVEVK+SAA MVFR
Sbjct: 121 KALGFIHKYIRSITLNHFGAESLRERFLPRIEESAKETLRYWATQPSVEVKDSAAVMVFR 180

Query: 181 TSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMK------------- 240
           TS+ KM SEDSSKLLTGGLTKKFTGLLGGFLTLP+N+PGTTYNKCMK             
Sbjct: 181 TSMVKMVSEDSSKLLTGGLTKKFTGLLGGFLTLPINVPGTTYNKCMKDMKEIQKKLREIL 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 EGRLASGAGSDEDFLGQAIKDKGSQQFISDDFIIQLLFSISFASFESISTTLTLVLNYLA 300

Query: 301 ----------AEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTT 360
                     AEHEAIR ARADPDGPITWEEYKSMTFTL+VI E LRLGSVTPALLRKTT
Sbjct: 301 DHPDVVKELEAEHEAIRNARADPDGPITWEEYKSMTFTLHVIFETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGL 399
           KE+QI GYTIPEGWTVMLVTASRHRDPAVYKDP TFNPWRWK+LDSIT+QKNFMPFGGGL
Sbjct: 361 KELQINGYTIPEGWTVMLVTASRHRDPAVYKDPHTFNPWRWKELDSITIQKNFMPFGGGL 420

[Close]

BLAST of Spg036685 vs. NCBI nr
Match: XP_023542446.1 (cytochrome P450 87A3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 716.1 bits (1847), Expect = 1.7e-202
Identity = 357/481 (74.22%), Postives = 376/481 (78.17%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MW IV+GLATLA+AYYIHWINKW+DSKFNGVLPPGTMGLPL+GET+QL+RPSDSLDVHPF
Sbjct: 1   MWAIVVGLATLAVAYYIHWINKWKDSKFNGVLPPGTMGLPLVGETLQLARPSDSLDVHPF 60

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           I+ KVKRYGPIFKTCLAGRPVVVSTDAEFN+YIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IKKKVKRYGPIFKTCLAGRPVVVSTDAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           KALG IHKYIRSITLNHFGAESLRERFLPRIEESA+ETL YW+TQ SVEVK+SAA MVFR
Sbjct: 121 KALGFIHKYIRSITLNHFGAESLRERFLPRIEESAKETLRYWATQPSVEVKDSAAVMVFR 180

Query: 181 TSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMK------------- 240
           TS+ KM SEDSSKLLTGGLTKKFTGLLGGFLTLP+N+PGTTYNKCMK             
Sbjct: 181 TSMVKMVSEDSSKLLTGGLTKKFTGLLGGFLTLPINVPGTTYNKCMKDMKEIQKKLREIL 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 EGRLASGGGSDEDFLGQAIKDKGSQQFISDDFIIQLLFSISFASFESISTTLTLVLNYLA 300

Query: 301 ----------AEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTT 360
                     AEHEAIR ARADPDGPITWEEYKSMTFTL+VI E LRLGSVTPALLRKTT
Sbjct: 301 DHPDVVKELEAEHEAIRNARADPDGPITWEEYKSMTFTLHVIFETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGL 399
           KE+QI GYTIPEGWTVMLVTASRHRDPAVYKDP TFNPWRWK+LDSIT+QKNFMPFGGGL
Sbjct: 361 KELQINGYTIPEGWTVMLVTASRHRDPAVYKDPHTFNPWRWKELDSITIQKNFMPFGGGL 420

[Close]

BLAST of Spg036685 vs. ExPASy Swiss-Prot
Match: K7NBR2 (Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=1 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 1.8e-191
Identity = 339/481 (70.48%), Postives = 359/481 (74.64%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWT+VLGLATL +AYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTVVLGLATLFVAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQ KV+RYGPIFKTCLAGRPVVVS DAEFN+YIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQKKVERYGPIFKTCLAGRPVVVSADAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAE+LRERFLP IE S+ E LH WSTQ SVEVK ++A MVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAEALRERFLPFIEASSMEALHSWSTQPSVEVKNASALMVFR 180

Query: 181 TSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMK------------- 240
           TSV KMF ED+ K L+G +  KFT LLGGFL+LPLN PGTTY+KC+K             
Sbjct: 181 TSVNKMFGEDAKK-LSGNIPGKFTKLLGGFLSLPLNFPGTTYHKCLKDMKEIQKKLREVV 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 DDRLANVGPDVEDFLGQALKDKESEKFISEEFIIQLLFSISFASFESISTTLTLILKLLD 300

Query: 301 ----------AEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTT 360
                     AEHEAIRKARADPDGPITWEEYKSMTFTL VI+E LRLGSVTPALLRKT 
Sbjct: 301 EHPEVVKELEAEHEAIRKARADPDGPITWEEYKSMTFTLQVINETLRLGSVTPALLRKTV 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGL 399
           K++Q+KGY IPEGWT+MLVTASRHRDP VYKDP  FNPWRWKDLDSIT+QKNFMPFGGGL
Sbjct: 361 KDLQVKGYIIPEGWTIMLVTASRHRDPKVYKDPHIFNPWRWKDLDSITIQKNFMPFGGGL 420

[Close]

BLAST of Spg036685 vs. ExPASy Swiss-Prot
Match: Q7XU38 (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 334.0 bits (855), Expect = 2.4e-90
Identity = 172/478 (35.98%), Postives = 246/478 (51.46%), Query Frame = 0


Query: 5   VLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPFIQSK 64
           +L  A  A+   + W  +W   + NG LPPG++GLP+IGET+Q   P+ + D+ PF++ +
Sbjct: 44  LLCAALAAVVALLRWAYRWSHPRSNGRLPPGSLGLPVIGETLQFFAPNPTCDLSPFVKER 103

Query: 65  VKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWLKALG 124
           +KRYG IFKT + GRPVVVS D E N+Y+  QEG+  E WY DT ++ FG D       G
Sbjct: 104 IKRYGSIFKTSVVGRPVVVSADPEMNYYVFQQEGKLFESWYPDTFTEIFGRDNVG-SLHG 163

Query: 125 LIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFRTSVA 184
            ++KY++++ L  +G E+L+   L   + + R +L  W++Q SVE+KE  + M+F  +  
Sbjct: 164 FMYKYLKTLVLRLYGQENLKSVLLAETDAACRGSLASWASQPSVELKEGISTMIFDLTAK 223

Query: 185 KMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCM------------------ 244
           K+   D SK     L K F   + G ++ PLN+PGT Y++CM                  
Sbjct: 224 KLIGYDPSKPSQVNLRKNFGAFICGLISFPLNIPGTAYHECMEGRKNAMKVLRGMMKERM 283

Query: 245 ------------------------------------------------------------ 304
                                                                       
Sbjct: 284 AEPERPCEDFFDHVIQELRREKPLLTETIALDLMFVLLFASFETTALALTIGVKLLTENP 343

Query: 305 ------KAEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTTKEI 364
                 + EHEAI + R DP+  +TW EYKSMTFT  VI E +RL ++ P + RK  +++
Sbjct: 344 KVVDALREEHEAIIRNRKDPNSGVTWAEYKSMTFTSQVIMEIVRLANIVPGIFRKALQDV 403

Query: 365 QIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSIT-VQKNFMPFGGGLRH 398
           +IKGYTIP GW +M+   + H +P +Y+DP  FNPWRW+    IT   K+FM FGGGLR 
Sbjct: 404 EIKGYTIPAGWGIMVCPPAVHLNPEIYEDPLAFNPWRWQGKPEITGGTKHFMAFGGGLRF 463

[Close]

BLAST of Spg036685 vs. ExPASy Swiss-Prot
Match: Q8L7D5 (Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3)

HSP 1 Score: 218.0 bits (554), Expect = 1.9e-55
Identity = 133/479 (27.77%), Postives = 223/479 (46.56%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           +W+  + +  +A      W+ +W + K NG LPPG+MGLP+IGET     P    ++ PF
Sbjct: 4   VWSAAVWVIAVAAVVISKWLYRWSNPKCNGKLPPGSMGLPIIGETCDFFEPHGLYEISPF 63

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           ++ ++ +YGP+F+T + G   VV T+ +    +  QE ++    Y +   K FG +  +L
Sbjct: 64  VKKRMLKYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGKENVFL 123

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           K  G IHK+++ I+L H G+E+L+++ +  I+    E L   + Q S + KE+  +++  
Sbjct: 124 KH-GNIHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMA 183

Query: 181 TSVAKMFS----EDSSKLLTGGLT---------KKFTGLL-------------------- 240
               K+ S    E  + L+   +           K T L+                    
Sbjct: 184 HLTPKIISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVF 243

Query: 241 ----------GGFL--------------------------------------TLPLNLPG 300
                     G FL                                      +L +    
Sbjct: 244 TRRKASREMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLA 303

Query: 301 TTYNKC--MKAEHEAIRKARADPDGPITWEEYK-SMTFTLNVISEALRLGSVTPALLRKT 360
             +     +K EH AI + R      ++WEEY+  MTFT  VI+E LR+ ++ P + RK 
Sbjct: 304 ENHKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMANMAPIMYRKA 363

Query: 361 TKEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGG 396
             +++IKGYTIP GW V ++  + H + A+Y++P  FNPWRW+  +  +  K FM FGGG
Sbjct: 364 VNDVEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWEGKELRSGSKTFMVFGGG 423

[Close]

BLAST of Spg036685 vs. ExPASy Swiss-Prot
Match: Q43147 (Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 6.2e-54
Identity = 134/455 (29.45%), Postives = 211/455 (46.37%), Query Frame = 0


Query: 22  KWRDSKFNGV-LPPGTMGLPLIGETIQLSRPSDSLDVHPFIQSKVKRYGPIFKTCLAGRP 81
           +W   K+N   LPPGTMG PL GET +  +   S     F++++  RYG  FK+ + G P
Sbjct: 23  RWNQVKYNQKNLPPGTMGWPLFGETTEFLKLGPS-----FMKNQRARYGSFFKSHILGCP 82

Query: 82  VVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWLKALGLIHKYIRSITLNHFGA 141
            +VS D+E N YI++ E + +   Y  ++    G         G  HKY+R   L+    
Sbjct: 83  TIVSMDSELNRYILVNEAKGLVPGYPQSMIDILG-KCNIAAVNGSAHKYMRGALLSLISP 142

Query: 142 ESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFRTSVAKMFSEDSSKLLTGGLT 201
             +R++ LP+I+E  R  L  W  +  ++++E    M F +S+ ++   +S+ L    ++
Sbjct: 143 TMIRDQLLPKIDEFMRSHLTNWDNK-VIDIQEKTNKMAFLSSLKQIAGIESTSLAQEFMS 202

Query: 202 KKFTGLLGGFLTLPLNLPGTTYNKCMKA-------------------------------- 261
            +F  L+ G L+LP+NLP T Y++  +A                                
Sbjct: 203 -EFFNLVLGTLSLPINLPNTNYHRGFQARKIIVNLLRTLIEERRASKEIQHDMLGYLMNE 262

Query: 262 --------------------------------------------------EHEAIRKARA 321
                                                             EH AIR+ + 
Sbjct: 263 EATRFKLTDDEMIDLIITILYSGYETVSTTSMMAVKYLHDHPKVLEELRKEHMAIRE-KK 322

Query: 322 DPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTTKEIQIKGYTIPEGWTVMLVTA 381
            P+ PI + +Y+SM FT  VI E  RL ++   +LRKTT++++I GY IP+GW + + T 
Sbjct: 323 KPEDPIDYNDYRSMRFTRAVILETSRLATIVNGVLRKTTQDMEINGYIIPKGWRIYVYTR 382

Query: 382 SRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGLRHCAGAEYSKVYLSTFLHILF 394
             + DP +Y DP +FNPWRW D  S+  Q +F+ FGGG R C G E     +STFLH   
Sbjct: 383 ELNYDPRLYPDPYSFNPWRWMD-KSLEHQNSFLVFGGGTRQCPGKELGVAEISTFLHYFV 442

[Close]

BLAST of Spg036685 vs. ExPASy Swiss-Prot
Match: Q5CCK3 (Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 5.2e-53
Identity = 136/452 (30.09%), Postives = 195/452 (43.14%), Query Frame = 0


Query: 32  LPPGTMGLPLIGETIQLSRPSDSLDVHPFIQSKVKRYGPIFKTCLAGRPVVVSTDAEFNH 91
           LPPG  G PL+GET    R   +  V  F++  + RYG I+++ L G   VVS DA  N 
Sbjct: 54  LPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGERTVVSADAGLNR 113

Query: 92  YIMLQEGRAVEMWYLDTLSKFFGLDTEW--LKALGLIHKYIRSITLNHFGAESLRERFLP 151
           YI+  EGR  E  Y  ++    G   +W  L  +G  H+ +R+I+LN   +  LR   LP
Sbjct: 114 YILQNEGRLFECSYPRSIGGILG---KWSMLVLVGDPHREMRAISLNFLSSVRLRAVLLP 173

Query: 152 RIEESARETLHYWSTQSSVEVKESAAAMVFRTSVAKMFSEDSSKLLTGGLTKKFTGLLGG 211
            +E      L  W   S+   +  A    F      + S D  +  T  L +++   + G
Sbjct: 174 EVERHTLLVLRAWPPSSTFSAQHQAKKFTFNLMAKNIMSMDPGEEETERLRREYITFMKG 233

Query: 212 FLTLPLNLPGTTYNKCMKA----------------------------------------- 271
            ++ PLNLPGT Y K +K+                                         
Sbjct: 234 VVSAPLNLPGTPYWKALKSRAAILGVIERKMEERVEKLSKEDASVEQDDLLGWALKQSNL 293

Query: 272 -------------------------------------------EHEAI-RKARADPDGPI 331
                                                      EH  I R+ R   +  +
Sbjct: 294 SKEQILDLLLSLLFAGHETSSMALALAIFFLEGCPKAVQELREEHLGIARRQRLRGECKL 353

Query: 332 TWEEYKSMTFTLNVISEALRLGSVTPALLRKTTKEIQIKGYTIPEGWTVMLVTASRHRDP 391
           +WE+YK M FT  VI+E LRLG+V   L RK  K++  KGY IP GW ++ V A+ H D 
Sbjct: 354 SWEDYKEMVFTQCVINETLRLGNVVRFLHRKVIKDVHYKGYDIPSGWKILPVLAAVHLDS 413

Query: 392 AVYKDPDTFNPWRWKDLDS---ITVQKNFMPFGGGLRHCAGAEYSKVYLSTFLHILFTKY 394
           ++Y+DP  FNPWRWK   S   +    +FMP+GGG R CAG+E +K+ ++ FLH L    
Sbjct: 414 SLYEDPQRFNPWRWKSSGSSGGLAQSSSFMPYGGGTRLCAGSELAKLEMAVFLHHL---- 473

[Close]

BLAST of Spg036685 vs. ExPASy TrEMBL
Match: A0A0A0LQP6 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa1G044890 PE=2 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 3.8e-208
Identity = 370/481 (76.92%), Postives = 381/481 (79.21%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTI+LGLATLAIAYYIHW+NKW+DSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQ KVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQRKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQ+SVEVKESAAAMVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQTSVEVKESAAAMVFR 180

Query: 181 TSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMK------------- 240
           TS+ KMFSEDSSKLLT GLTKKFTGLLGGFLTLPLNLPGTTY+KC+K             
Sbjct: 181 TSIVKMFSEDSSKLLTEGLTKKFTGLLGGFLTLPLNLPGTTYHKCIKDMKQIQKKLKDIL 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 EERLAKGVKIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300

Query: 301 ----------AEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTT 360
                     AEHEAIRKARADPDGPITWEEYKSM FTLNVI E LRLGSVTPALLRKTT
Sbjct: 301 DHPDVVKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGL 399
           KEIQIKGYTIPEGWTVMLVTASRHRDP VYKDPDTFNPWRWK+LDSIT+QKNFMPFGGGL
Sbjct: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQKNFMPFGGGL 420

[Close]

BLAST of Spg036685 vs. ExPASy TrEMBL
Match: A0A5D3BH60 (Cytochrome P450 87A3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002960 PE=3 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 5.0e-208
Identity = 369/481 (76.72%), Postives = 381/481 (79.21%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTI+LGLATLAIAYYIHW+NKW+DSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQ SVEVKESAAAMVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQPSVEVKESAAAMVFR 180

Query: 181 TSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMK------------- 240
           TS+ KMFSEDSSKLLT GLTKKFTGLLGGFLTLPLN+PGTTY+KC+K             
Sbjct: 181 TSIVKMFSEDSSKLLTAGLTKKFTGLLGGFLTLPLNVPGTTYHKCIKDMKEIQKKLKDIL 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 EERLAKGVSIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300

Query: 301 ----------AEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTT 360
                     AEHEAIRKARADPDGPITWEEYKSM FTLNVI E LRLGSVTPALLRKTT
Sbjct: 301 DHPDVAKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGL 399
           KEIQIKGYTIPEGWTVMLVTASRHRDP VYKDPDTFNPWRWK+LDSIT+Q+NFMPFGGGL
Sbjct: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQRNFMPFGGGL 420

[Close]

BLAST of Spg036685 vs. ExPASy TrEMBL
Match: A0A1S3AUF5 (cytochrome P450 87A3-like OS=Cucumis melo OX=3656 GN=LOC103482802 PE=3 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 5.0e-208
Identity = 369/481 (76.72%), Postives = 381/481 (79.21%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTI+LGLATLAIAYYIHW+NKW+DSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF
Sbjct: 1   MWTILLGLATLAIAYYIHWVNKWKDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQ SVEVKESAAAMVFR
Sbjct: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQPSVEVKESAAAMVFR 180

Query: 181 TSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMK------------- 240
           TS+ KMFSEDSSKLLT GLTKKFTGLLGGFLTLPLN+PGTTY+KC+K             
Sbjct: 181 TSIVKMFSEDSSKLLTAGLTKKFTGLLGGFLTLPLNVPGTTYHKCIKDMKEIQKKLKDIL 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 EERLAKGVSIDEDFLGQAIKDKESQQFISEEFIIQLLFSISFASFESISTTLTLILNFLA 300

Query: 301 ----------AEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTT 360
                     AEHEAIRKARADPDGPITWEEYKSM FTLNVI E LRLGSVTPALLRKTT
Sbjct: 301 DHPDVAKELEAEHEAIRKARADPDGPITWEEYKSMNFTLNVICETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGL 399
           KEIQIKGYTIPEGWTVMLVTASRHRDP VYKDPDTFNPWRWK+LDSIT+Q+NFMPFGGGL
Sbjct: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPEVYKDPDTFNPWRWKELDSITIQRNFMPFGGGL 420

[Close]

BLAST of Spg036685 vs. ExPASy TrEMBL
Match: A0A6J1GV28 (cytochrome P450 87A3-like OS=Cucurbita moschata OX=3662 GN=LOC111457186 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 7.0e-202
Identity = 356/481 (74.01%), Postives = 375/481 (77.96%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MW IV+GLATLA+AYYIHWINKW+DSKFNGVLPPGTMGLPL+GET+QL+RPSDSLDVHPF
Sbjct: 1   MWAIVVGLATLAVAYYIHWINKWKDSKFNGVLPPGTMGLPLVGETLQLARPSDSLDVHPF 60

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           I+ KVKRYG IFKTCLAGRPVVVSTDAEFN+YIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IKKKVKRYGSIFKTCLAGRPVVVSTDAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           KALG IHKYIRSITLNHFGAESLRERFLPRIEESA+ETL YW+TQ SVEVK+SAA MVFR
Sbjct: 121 KALGFIHKYIRSITLNHFGAESLRERFLPRIEESAKETLRYWATQPSVEVKDSAAVMVFR 180

Query: 181 TSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMK------------- 240
           TS+ KM SEDSSKLLTGGLTKKFTGLLGGFLTLP+N+PGTTYNKCMK             
Sbjct: 181 TSMVKMVSEDSSKLLTGGLTKKFTGLLGGFLTLPINVPGTTYNKCMKDMKEIQKKLREIL 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 EGRLASGAGSDEDFLGQAIKDKGSQQFISDDFIIQLLFSISFASFESISTTLTLVLNYLA 300

Query: 301 ----------AEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTT 360
                     AEHEAIR ARADPDGPITWEEYKSMTFTL+VI E LRLGSVTPALLRKTT
Sbjct: 301 DHPDVVKELEAEHEAIRNARADPDGPITWEEYKSMTFTLHVIFETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGL 399
           KE+QI GYTIPEGWTVMLVTASRHRDPAVYKDP TFNPWRWK+LDSIT+QKNFMPFGGGL
Sbjct: 361 KELQINGYTIPEGWTVMLVTASRHRDPAVYKDPHTFNPWRWKELDSITIQKNFMPFGGGL 420

[Close]

BLAST of Spg036685 vs. ExPASy TrEMBL
Match: A0A6J1K5M4 (cytochrome P450 87A3-like OS=Cucurbita maxima OX=3661 GN=LOC111490271 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 9.1e-202
Identity = 357/481 (74.22%), Postives = 376/481 (78.17%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MWTIV+GLATLA+AYYIHWINKW+DSKFNGVLPPGTMGLPLIGET+QLSRPSDSLDVHPF
Sbjct: 1   MWTIVVGLATLAVAYYIHWINKWKDSKFNGVLPPGTMGLPLIGETLQLSRPSDSLDVHPF 60

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           I+ KVKRYG IFKTCLAGRPVVVSTDAEFN+YIMLQEGRAVEMWYLDTLSKFFGLDTEWL
Sbjct: 61  IKKKVKRYGSIFKTCLAGRPVVVSTDAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           KALG IHKYIRSITLNHFGAESLRERFLPRIEESA+ETL YW+TQ SVEVK+SAA MVFR
Sbjct: 121 KALGFIHKYIRSITLNHFGAESLRERFLPRIEESAKETLCYWATQPSVEVKDSAAVMVFR 180

Query: 181 TSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMK------------- 240
           TS+ KM S+DSSKLLTGGLTKKFTGLLGGFLTLP+N+PGTTYNKCMK             
Sbjct: 181 TSMVKMVSKDSSKLLTGGLTKKFTGLLGGFLTLPINVPGTTYNKCMKDMKEIQKKLREIL 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 EGRLASGAGSDEDFLGQAVKDKGSQKFISDDFIIQLLFSISFASFESISTTLTLILNYLA 300

Query: 301 ----------AEHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKTT 360
                     AEHEAIR ARADPDGPITWEEYKSMTFTL+VI E LRLGSVTPALLRKTT
Sbjct: 301 DHPDVVKELEAEHEAIRNARADPDGPITWEEYKSMTFTLHVIFETLRLGSVTPALLRKTT 360

Query: 361 KEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGGL 399
           KE+QI GYTIPEGWTVMLVTASRHRDPAVYKDP TFNPWRWK+LDSIT+QKNFMPFGGGL
Sbjct: 361 KELQINGYTIPEGWTVMLVTASRHRDPAVYKDPHTFNPWRWKELDSITIQKNFMPFGGGL 420

[Close]

BLAST of Spg036685 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 331.3 bits (848), Expect = 1.1e-90
Identity = 170/481 (35.34%), Postives = 252/481 (52.39%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MW +++ ++ L I+   HW+  WR+ K  G LPPG+MG PL+GE+IQ  +P+ + D+ PF
Sbjct: 1   MWALLIWVSLLLIS-ITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPF 60

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           I+ +VK+YGPIFKT L GRPV+VSTDA+ ++++  QEGR  + WY DT +  FG      
Sbjct: 61  IKERVKKYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVG- 120

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
              G ++KY++++ L  FG + L+ + LP++E +A + L  WS Q SVE+K++ A+M+F 
Sbjct: 121 SLHGFMYKYLKNMVLTLFGHDGLK-KMLPQVEMTANKRLELWSNQDSVELKDATASMIFD 180

Query: 181 TSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMKA------------ 240
            +  K+ S D  K  +  L   F   + G ++ P ++PGT Y+KC++             
Sbjct: 181 LTAKKLISHDPDK-SSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNML 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 QERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFL 300

Query: 301 ------------EHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTPALLRKT 360
                       EHE I + R D D  +TWEEYKSMT+T   I+E  RL ++ PA+ RK 
Sbjct: 301 SDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKA 360

Query: 361 TKEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGGG 398
            ++I+ K YTIP GW VM+   + H +P +YKDP  FNP RW+        K+FM FGGG
Sbjct: 361 LRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGG 420

[Close]

BLAST of Spg036685 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 325.9 bits (834), Expect = 4.6e-89
Identity = 171/487 (35.11%), Postives = 252/487 (51.75%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           MW +++ ++ L I+   HW+  WR+ K  G LPPG+MG PL+GE+IQ  +P+ + D+ PF
Sbjct: 1   MWALLIWVSLLLIS-ITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPF 60

Query: 61  IQSKVK------RYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFG 120
           I+ +VK      RYGPIFKT L GRPV+VSTDA+ ++++  QEGR  + WY DT +  FG
Sbjct: 61  IKERVKNDVDMCRYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFG 120

Query: 121 LDTEWLKALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESA 180
                    G ++KY++++ L  FG + L+ + LP++E +A + L  WS Q SVE+K++ 
Sbjct: 121 KKNVG-SLHGFMYKYLKNMVLTLFGHDGLK-KMLPQVEMTANKRLELWSNQDSVELKDAT 180

Query: 181 AAMVFRTSVAKMFSEDSSKLLTGGLTKKFTGLLGGFLTLPLNLPGTTYNKCMKA------ 240
           A+M+F  +  K+ S D  K  +  L   F   + G ++ P ++PGT Y+KC++       
Sbjct: 181 ASMIFDLTAKKLISHDPDK-SSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMK 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 MLRNMLQERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALT 300

Query: 301 ------------------EHEAIRKARADPDGPITWEEYKSMTFTLNVISEALRLGSVTP 360
                             EHE I + R D D  +TWEEYKSMT+T   I+E  RL ++ P
Sbjct: 301 LAIKFLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVP 360

Query: 361 ALLRKTTKEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNF 398
           A+ RK  ++I+ K YTIP GW VM+   + H +P +YKDP  FNP RW+        K+F
Sbjct: 361 AIFRKALRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHF 420

[Close]

BLAST of Spg036685 vs. TAIR 10
Match: AT3G44970.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 248.1 bits (632), Expect = 1.2e-65
Identity = 148/446 (33.18%), Postives = 221/446 (49.55%), Query Frame = 0


Query: 2   WTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPFI 61
           W     +  L +A   HW  +W + K NG LPPG+MG P+IGET+   +P    ++ P++
Sbjct: 5   WNTGFCVIVLVVARVGHWWYQWSNPKSNGKLPPGSMGFPIIGETLDFFKPYGFYEISPYL 64

Query: 62  QSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWLK 121
           + K+ RYGP+F+T + G   VVSTD + N  I+ QE ++  + Y D L K  G D+ +LK
Sbjct: 65  KKKMLRYGPLFRTNILGVKTVVSTDKDVNMEILRQENKSFILSYPDGLMKPLGKDSLFLK 124

Query: 122 ALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFRT 181
            +G IHK+I+ ITL+   +E L+ + L  ++   RE L   +    ++VK++ + ++   
Sbjct: 125 -IGNIHKHIKQITLHLLSSEGLKRKILKDMDRVTREHLSSKAKTGRLDVKDAVSKLIIAH 184

Query: 182 SVAKMFS----EDSSKLLTGGLTKKFT--------------GLL---------------- 241
              KM S    +  +KL+  G+ K FT              GL                 
Sbjct: 185 LTPKMMSNLKPQTQAKLM--GIFKAFTFDWFRTSYLISAGKGLYNTLWACREGMREIKDI 244

Query: 242 -------------------------------GGFLTLPLNLPGTTYNKCMKA-------- 301
                                             +TL   L   T +   KA        
Sbjct: 245 YTMRKTSEEKYDDFLNTAIEESEKAGELLNENAIITLIFTLSCVTQDTTSKAICLAVKFL 304

Query: 302 ------------EHEAIRKARADPDGPITWEEYK-SMTFTLNVISEALRLGSVTPALLRK 361
                       EHE I ++R D +G +TWEEY+  MTFT  VI+E+LR+ ++ P L RK
Sbjct: 305 LENPKVLAELKKEHEVILESREDKEGGVTWEEYRHKMTFTNMVINESLRITNLAPMLFRK 364

[Close]

BLAST of Spg036685 vs. TAIR 10
Match: AT1G55940.1 (cytochrome P450, family 708, subfamily A, polypeptide 1 )

HSP 1 Score: 237.3 bits (604), Expect = 2.2e-62
Identity = 150/498 (30.12%), Postives = 234/498 (46.99%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIH-WINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHP 60
           MW + L      +   I  W+ +W +   +G LPPG+MG P+IGET++  +P    ++HP
Sbjct: 148 MWGVALSFVIALVVVKISLWLYRWANPNCSGKLPPGSMGFPVIGETVEFFKPYSFNEIHP 207

Query: 61  FIQSKV-KRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTE 120
           F++ ++ K  G +F+T + G   +VSTD E N  I+ QE R   M Y + L + FG D  
Sbjct: 208 FVKKRMFKHGGSLFRTNILGSKTIVSTDPEVNFEILKQENRCFIMSYPEALVRIFGKDNL 267

Query: 121 WLKALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMV 180
           + K     H+Y+R I L   G E L++RF+ +I+ +  E L   S Q  V+VK+++  ++
Sbjct: 268 FFKQGKDFHRYMRHIALQLLGPECLKQRFIQQIDIATSEHLKSVSFQGVVDVKDTSGRLI 327

Query: 181 FRTSVAKMFS----EDSSKLLTG------GLTKK----------FTGLL----------- 240
               +  + S    E  SKL+         L +           + GL+           
Sbjct: 328 LEQMILMIISNIKPETKSKLIESFRDFSFDLVRSPFDPSFWNALYNGLMDFVWLLNILDP 387

Query: 241 --------------------------------GGFL--------------------TLPL 300
                                           G F+                     L L
Sbjct: 388 LFYVKARSNVMKMLKRMFKERREEATSDDSKYGDFMETMIYEVEKEGDTINEERSVELIL 447

Query: 301 NLPGTTYNKC--------------------MKAEHEAIRKARADPDGPITWEEYKS-MTF 360
           +L   +Y                       +K EHE I + RAD +  +TW+EY+S M F
Sbjct: 448 SLLIASYETTSTMTALTVKFIAENPKVLMELKREHETILQNRADKESGVTWKEYRSMMNF 507

Query: 361 TLNVISEALRLGSVTPALLRKTTKEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFN 393
           T  VI+E+LRLGS++PA+ RK   +++IKGYTIP GW V++V +  H DP +Y+ P  FN
Sbjct: 508 THMVINESLRLGSLSPAMFRKAVNDVEIKGYTIPAGWIVLVVPSLLHYDPQIYEQPCEFN 567

[Close]

BLAST of Spg036685 vs. TAIR 10
Match: AT1G78490.1 (cytochrome P450, family 708, subfamily A, polypeptide 3 )

HSP 1 Score: 232.6 bits (592), Expect = 5.3e-61
Identity = 139/446 (31.17%), Postives = 215/446 (48.21%), Query Frame = 0


Query: 1   MWTIVLGLATLAIAYYIHWINKWRDSKFNGVLPPGTMGLPLIGETIQLSRPSDSLDVHPF 60
           +W + + +  L +    HW+ +W + K  G LPPG+MG P+IGET+   +P     +  F
Sbjct: 4   IWNVAMLMVALVVVRISHWLYRWSNPKCPGKLPPGSMGFPIIGETLDFFKPCGVEGIPTF 63

Query: 61  IQSKVKRYGPIFKTCLAGRPVVVSTDAEFNHYIMLQEGRAVEMWYLDTLSKFFGLDTEWL 120
           ++ ++ RYGP+F+T + G   VVSTD +  H I  QE  + E+ Y D   K FG D  +L
Sbjct: 64  VKKRMIRYGPLFRTNIFGSKTVVSTDPDVIHQIFRQENTSFELGYPDIFVKVFGKDNLFL 123

Query: 121 KALGLIHKYIRSITLNHFGAESLRERFLPRIEESARETLHYWSTQSSVEVKESAAAMVFR 180
           K +  IHKY++ IT+   G+E L++  L  ++++ R+ +   ++Q S  V++    +V  
Sbjct: 124 KEV-FIHKYLQKITMQILGSEGLKQTMLGNMDKATRDHIRSIASQGSFNVRKEVENLVVA 183

Query: 181 TSVAKMFS----EDSSKLLTG-------------------GLTK---------------- 240
               K+ S    E  SKL+                      +TK                
Sbjct: 184 YMTPKLISNLKPETQSKLIDNLNAFNLDWFKSFLRLSTWKAVTKALKSREEAIQVMKDVL 243

Query: 241 --------KFTGLLGGFLTLPLNLPGTTYNK--------------------C-------- 300
                   K    L   L   L   G+ +++                    C        
Sbjct: 244 MMRKETREKQEDFLNTLLE-ELEKDGSFFDQGSAINLIFLLAFALREGTSSCTALAVKFI 303

Query: 301 ---------MKAEHEAIRKARADPDGPITWEEYK-SMTFTLNVISEALRLGSVTPALLRK 360
                    +K EH+AI   R D +  ++WEEY+ +MTFT  V +E LRL + TP L RK
Sbjct: 304 SKDPKVLAELKREHKAIVDNRKDKEAGVSWEEYRHNMTFTNMVSNEVLRLANTTPLLFRK 363

Query: 361 TTKEIQIKGYTIPEGWTVMLVTASRHRDPAVYKDPDTFNPWRWKDLDSITVQKNFMPFGG 362
             ++++IKGYTIP GW V +  ++ H DPA+Y++P  FNPWRW+  + I   K FM FG 
Sbjct: 364 AVQDVEIKGYTIPAGWIVAVAPSAVHFDPAIYENPFEFNPWRWEGKEMIWGSKTFMAFGY 423

The following BLAST results are available for this feature:

BLAST of Spg036685 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895732.1	4.2e-209	77.13	cucurbitadienol 11-hydroxylase [Benincasa hispida]	[more]
NP_001289636.1	7.9e-208	76.92	Cytochrome P450 87A3-like [Cucumis sativus] >AIT72037.1 cytochrome P450 [Cucumis...	[more]
XP_008437370.1	1.0e-207	76.72	PREDICTED: cytochrome P450 87A3-like [Cucumis melo] >TYJ99092.1 cytochrome P450 ...	[more]
KAG6573008.1	1.7e-202	74.22	Cytochrome P450 87A3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023542446.1	1.7e-202	74.22	cytochrome P450 87A3-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg036685 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
K7NBR2	1.8e-191	70.48	Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=...	[more]
Q7XU38	2.4e-90	35.98	Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV...	[more]
Q8L7D5	1.9e-55	27.77	Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3	[more]
Q43147	6.2e-54	29.45	Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1	[more]
Q5CCK3	5.2e-53	30.09	Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV...	[more]

BLAST of Spg036685 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LQP6	3.8e-208	76.92	Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa1G044890 PE=2 SV=1	[more]
A0A5D3BH60	5.0e-208	76.72	Cytochrome P450 87A3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3AUF5	5.0e-208	76.72	cytochrome P450 87A3-like OS=Cucumis melo OX=3656 GN=LOC103482802 PE=3 SV=1	[more]
A0A6J1GV28	7.0e-202	74.01	cytochrome P450 87A3-like OS=Cucurbita moschata OX=3662 GN=LOC111457186 PE=3 SV=...	[more]
A0A6J1K5M4	9.1e-202	74.22	cytochrome P450 87A3-like OS=Cucurbita maxima OX=3661 GN=LOC111490271 PE=3 SV=1	[more]

BLAST of Spg036685 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12740.1	1.1e-90	35.34	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]
AT1G12740.2	4.6e-89	35.11	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]
AT3G44970.1	1.2e-65	33.18	Cytochrome P450 superfamily protein	[more]
AT1G55940.1	2.2e-62	30.12	cytochrome P450, family 708, subfamily A, polypeptide 1	[more]
AT1G78490.1	5.3e-61	31.17	cytochrome P450, family 708, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002397	Cytochrome P450, B-class	PRINTS	PR00359	BP450	coord: 257..268 score: 29.81 coord: 331..340 score: 40.67 coord: 274..301 score: 24.13 coord: 302..317 score: 41.11 coord: 340..351 score: 27.59
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 24..224 e-value: 1.4E-16 score: 62.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 225..397 e-value: 1.4E-38 score: 134.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 32..397
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 33..189 e-value: 2.7E-7 score: 29.7 coord: 225..365 e-value: 4.8E-32 score: 111.3
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 227..369 coord: 9..227
None	No IPR available	PANTHER	PTHR24286:SF90	CYTOCHROME P450 87A3-LIKE	coord: 227..369 coord: 9..227
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 333..342

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg036685.1	Spg036685.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016712	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen