Spg036540 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg036540
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Cystatin domain-containing protein
Location	scaffold5: 47975029 .. 47978500 (-)
RNA-Seq Expression	Spg036540
Synteny	Spg036540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGATCATCAATGTAAACCAACCTCGTAGGTTTTTCGCTGCTCTCGTTCTGGAAAATGGAACCGGCACCTCGAAACCCTTGAAGGACGGGCTTCAATAAAGAAGTACTAGAACCCACTTCCGGAATCGACGACTTCAATTGCAGCGACGAGGAAGAGCGGCAAGTGAGTTCGATATTCGCCGAGTTGTCGTTTGGGGTTGTAGAATCCATTATCGAGCTTGTGGTTGATGAAAACCTAAATGCAGTAATGGAGATTGAACGTGGACTAGAGAGCATCATTGCAGAAAACGTACCAGCGACTGTATGAAAACGAAGAAGAAAGAGAGAGACGAGTCTTCGGTCTCATGGTTTCGATTTTTTTTAATTCAGATGAATCGGAACTGCCGAGAGAGACACTGACTGAGAAAAAGTATTGTCAATCAATTTAATTTCAAGGTTCAATTGATTTATACCATATAGAAGCATTTTTTCATCATATATATACACTAGTATGAATTATATATGAGTTAGTTCGTAAAATATATAGCATGTTTGTGATGGCCGAATAATATATATACCTAGTTGTTATGAATAAATGGTAAAGAGAATTCCAAAAAAATAAAGGTGAAATAGTTAACGACTATCAGGGGTATTTATTTTGTATGTTACAATTGTTTGTTTAACACATTATCAGTAAATGAAAATATATGTTTTGGTTTTGAATATATAAATTATGTCTCGTTTATATTTTTGGTGATAAGGTGGTTTTGTTGAAACATAGAATATATACCCCTGTTTTAGAATTGAAACATTACTTCTGCTCCGCTCGTCTGCTTGATTAGATCTAAAACTCTTTGTGATTATGTTTGCAAAAAAAGATTCCCCTCCTTTGTTGGCTCTCTGTTGGCTATCTTCTTTGCTTTTACATCCTGTGACTCTTGAAATGCCACAGTATTAGAGTAGTCATTCATTGTTGTTTAGTTGAGTTTGAATCTGTTAAGAGAGCTCATTAAAGAACAAAGCTGGAAGTGTCAATGGTCTGTTATTTAGTCTCATAGATTGCAACTGATTGAGTTTTTTATATTATGTTTTTCAGTTGTAAATATATCGTTTATATTGTATTCTCAACGTGCCTACACTAAAGAACAAAGTTGGAAGTATCAATTGTCTGTTATTTAGTCTCTTAGATTGCAACTTTGCAACTGATTGAGTTTGTTATATTATGTTTTTCAGTTGTAAAATTTATTGTTTATATTGTATTCTCAGTGTGCCACTGTGCCTATATAAGGGCCACTGATTTGATCACTCATCAATGAAGAATGATTTATATTTGTGATCATTGAAGTTCTTGAGTTTAGTTTCATTCTTCATCTTAATAGATACTGTTTGCAAAATAAACGTGCAGTTTTGATTAAATTGTGTAAGTTGTTTGTAGCATCTCTTGGAGCAATTACGTGCAAGTGATCATTAGAATTTGAATTACAATCTCATTAATTTCTTATTTTAGTAGTGTATGATTTTCCATTTCTATTCGTTTTATTATTTAACATGACAAGCAGTTATGCGCCGTAATTTTGTGAGCCTTCGTCTTGTGACATTGTGCTTTTAAAGACTCTATCCTTCTTCTTTTTCTCCTTCATCTTCTCCTCCTTCTTTCAAGCAAGGATGAGTAAGAAGTCTTTGTTTTTTCTGGTTTTAGATTAGAAATCTTACACTTGAGCTGAGTCCAGCTCAGAGCCTGCTTAGTCTCTCTTCCATAATTTAAAGCACATGTTGTGCTAGCTTGCTTCCCTATTGCCTCATAGATGACCTTCTTCATTGACCCCACAACCAAAGTTCACACTTTCCCTCTTCCACACAACTTCACTGATAAAATGCCAGAAAACAATGGGGCGTGGGAGAACATTTCATCTTCTGTATGGGGAATTGGGTGGATTTTCTTGTTTCTGTGGTTGTTGTTCATTTCCAGACATTTGTTAAAATTTTTCTAGATTGAAATTGCAAAGTGAGTTTTCCCTCTATAGTCCTCTTTGTATTAGATACTTTAACTTTTATATTTTTTTTCTAATGCATGTTATATCTTTTTTATATTATATATACTAAATTCTATTTAAACTATAAAAGGAAAAAAAAGGAAACAAGATACAGAAACAGTTATCAAACAAGTTTCTGCCTCTGCGAACAATTATCTTCATGAATTAGTTGGATATTTTATAATTGTTACAAAATTTTAATGATAGTTAACTTCTTGGTGTTTGGTTTTCATGTTCATGATATTGTTTTCCATTCTCTTTCTCTGGTTTGGCTCTTTAACGATTACTAGTGGATTATATTAACTTTCGGAGGTCAAGATCCTTTTAGCCTTTAATAATCAAGTCATTTCCGGTTTTCATTTTTATATGCATTTTGTACCTTAATCCTTCCCCCTATTTATGAATCAATTAGGCACTGTTTGATAATCATTTGGTTTTTGAAAATTATGTTTGTTTTCTTCCAATTTCCTATCAGAGCACATTCGAATTCTTAGATGGTTTTCAAGTATAACGTGACGTCTCATTATTTGACTTTGTTCTGAAATGAAGATTTCGAAATTAAAATTACAGAGGAGGATGGCGGCTTGTTCTTCTCCAGCCAAACTCAGCCCATCAGATGATGATTCACATAATTATGATGAATTTTCTATTGATGACGGTACCCGTGACATGACTGATGAAGAGATGTCCGAATACGTTCGTGCCTTCAAAGAAACCGAGGTTTAGTTTCTTTAGTTTCTTGCTTGATTTTCGTTCAATAATGATTGAAATTAATTAATCACAGGGTTTTGATATGCCCTCTTTTCCTGATACCGTAGCTGTCAGCCGTATTGAGTGTATTACAGGGCATCGATTATATACAGAAGAAAATATTTCAGAAGGCTCAAAGATAGCTATTAATCACTACAACAGGCAAAATGTATGTATTCTTTTCTCTTTGTTTAAAAAGGAAAAAGAAGAAAGGTTTTTGTTTTCATGTTTCAAAATTATGTACTCTGTAGGGTACAAGTTTTGAATTCGTGAAGATTGTGAAGGCCAATAATCAAGCTGCACGGGGTATTTTGTATTACCTCACTTTTGAGGCCAAGCCAAGAGGAACACCTCGAGATTTCCCCACCACAACGTTTCAAGCTTGGGTTCTCTTTTTCATTGATGAAGTAGAGGTCCAACTTTGCAGGCCAAAACCTTCTACTTGATCACCTGTTAGACCCCAATTAAATGCACTTATAGTAGGAAGGGAATTGTGAGCTTTTGTAGGAGTCTGGTGCGTTTTCTTGTTTAGGTTCAGGGTTTAGGGTATAGAACTCTAAATAGAACAGTATTGATCTAATTTTTCCCTGTTTGTGGGTTGTTTTAGTTTTTTTTTTTTTTTTTGTTAGGATACCTAACATACCACTGCCAACTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGAACATCAACATTTGGGAGTGGGGGGATT

mRNA sequence

ATGGCGGCTTGTTCTTCTCCAGCCAAACTCAGCCCATCAGATGATGATTCACATAATTATGATGAATTTTCTATTGATGACGGTACCCGTGACATGACTGATGAAGAGATGTCCGAATACGTTCGTGCCTTCAAAGAAACCGAGGGTTTTGATATGCCCTCTTTTCCTGATACCGTAGCTGTCAGCCGTATTGAGTGTATTACAGGGCATCGATTATATACAGAAGAAAATATTTCAGAAGGCTCAAAGATAGCTATTAATCACTACAACAGGCAAAATGGTACAAGTTTTGAATTCGTGAAGATTGTGAAGGCCAATAATCAAGCTGCACGGGGTATTTTGTATTACCTCACTTTTGAGGCCAAGCCAAGAGGAACACCTCGAGATTTCCCCACCACAACGTTTCAAGCTTGGGTTCTCTTTTTCATTGATGAAGTAGAGGTCCAACTTTGCAGGCCAAAACCTTCTACTTGA

Coding sequence (CDS)

ATGGCGGCTTGTTCTTCTCCAGCCAAACTCAGCCCATCAGATGATGATTCACATAATTATGATGAATTTTCTATTGATGACGGTACCCGTGACATGACTGATGAAGAGATGTCCGAATACGTTCGTGCCTTCAAAGAAACCGAGGGTTTTGATATGCCCTCTTTTCCTGATACCGTAGCTGTCAGCCGTATTGAGTGTATTACAGGGCATCGATTATATACAGAAGAAAATATTTCAGAAGGCTCAAAGATAGCTATTAATCACTACAACAGGCAAAATGGTACAAGTTTTGAATTCGTGAAGATTGTGAAGGCCAATAATCAAGCTGCACGGGGTATTTTGTATTACCTCACTTTTGAGGCCAAGCCAAGAGGAACACCTCGAGATTTCCCCACCACAACGTTTCAAGCTTGGGTTCTCTTTTTCATTGATGAAGTAGAGGTCCAACTTTGCAGGCCAAAACCTTCTACTTGA

Protein sequence

MAACSSPAKLSPSDDDSHNYDEFSIDDGTRDMTDEEMSEYVRAFKETEGFDMPSFPDTVAVSRIECITGHRLYTEENISEGSKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKPRGTPRDFPTTTFQAWVLFFIDEVEVQLCRPKPST

Homology

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BLAST of Spg036540 vs. NCBI nr
Match: XP_022137526.1 (uncharacterized protein LOC111008952 [Momordica charantia] >XP_022137527.1 uncharacterized protein LOC111008952 [Momordica charantia])

HSP 1 Score: 144.1 bits (362), Expect = 1.1e-30
Identity = 86/167 (51.50%), Postives = 111/167 (66.47%), Query Frame = 0


Query: 1   MAACSSPAKLSPSDDDSHN-------YDEFSIDDGTRDMTDEEMSEYVRAFKETEGFDMP 60
           MA+ +SP +LS  DDD  +       +D+    +  R+MT EE  EY  A K+TEGFDMP
Sbjct: 1   MASSNSP-QLSDDDDDFGDEPREMTIHDDAYFGNEPREMTLEEEIEYYIALKKTEGFDMP 60

Query: 61  SFPDTVAVSRIECITGHRLYTEENISEGSK-IAINHYNRQNGTSFEFVKIVKANNQAARG 120
           SFPD+ A  RIE ITG RL++EE      K  A++++N+QNGTSFEFVK+VKA N+A  G
Sbjct: 61  SFPDSYACGRIETITGSRLHSEELQECADKDKAVDYFNQQNGTSFEFVKMVKATNKAVVG 120

Query: 121 ILYYLTFEAKPRGTPRDFPTTTFQAWVL--FFIDEVEVQLCRPKPST 158
           I+YYLTFE K  G+P + PT T QA VL    I   +V+LCRP+PST
Sbjct: 121 IMYYLTFEVKQMGSPPNSPTKTLQARVLRGLPIGHFDVELCRPEPST 166

[Close]

BLAST of Spg036540 vs. NCBI nr
Match: XP_022137488.1 (uncharacterized protein LOC111008920 isoform X1 [Momordica charantia])

HSP 1 Score: 132.9 bits (333), Expect = 2.4e-27
Identity = 74/150 (49.33%), Postives = 103/150 (68.67%), Query Frame = 0


Query: 10  LSPSDDDSHNYD-EFSIDDGTRDMTDEEMSEYVRAFKETEGFDMPSFPDTVAVSRIECIT 69
           LS   ++++ YD +   DDG R+M +EE++ Y +A +E+EGFD+PSFP T A + I  + 
Sbjct: 33  LSVPANNNYLYDSDGYYDDGVREMNEEEVNRYYKAIQESEGFDVPSFPGTYAFAIISPLP 92

Query: 70  GHRLYTEENISEGSKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKPRGTPR 129
              L +EE + E +  AI HYN +NG SFEFVK++KAN+QAA G L++LTF+ K  G P 
Sbjct: 93  LINLISEE-VRECAGQAIKHYNNENGASFEFVKMLKANSQAALGELFFLTFQVKQTGAPP 152

Query: 130 DFPTTTFQAWVLFFI--DEVEVQLCRPKPS 157
           D PTTT QA VL  I  D+ +VQLCRP+P+
Sbjct: 153 DSPTTTLQARVLAGISPDDFDVQLCRPEPT 181

[Close]

BLAST of Spg036540 vs. NCBI nr
Match: XP_023525925.1 (uncharacterized protein LOC111789396 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 120.2 bits (300), Expect = 1.6e-23
Identity = 69/145 (47.59%), Postives = 93/145 (64.14%), Query Frame = 0


Query: 14  DDDSHNYDEFSIDDGTRDMTDEEMSEYVRAFKETEGFDMPSFPDTVAVSRIECITGHRLY 73
           DD    Y+      G R M  EEMS Y +  ++T+GFD+P+FP+T A+  I  I    L 
Sbjct: 47  DDSDEEYEH-----GVRVMNREEMSRYYQVLRDTDGFDVPTFPNTYALGLIVPICEEYL- 106

Query: 74  TEENISEGSKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKPRGTPRDFPTT 133
           ++  + + ++ AI+HYN +NGT+FEFVKIVKAN Q   G  YY+TF+ K  GT  +FPTT
Sbjct: 107 SKPYLRDCAEKAISHYNIRNGTNFEFVKIVKANQQVVSGFFYYITFDVKQIGT--EFPTT 166

Query: 134 TFQAWVLFFIDEV-EVQLCRPKPST 158
           TF+A VL  ID+  EV LCRPK S+
Sbjct: 167 TFEAKVLDGIDDTKEVYLCRPKRSS 183

[Close]

BLAST of Spg036540 vs. NCBI nr
Match: XP_023521681.1 (UPF0725 protein At4g29550-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 113.6 bits (283), Expect = 1.5e-21
Identity = 64/154 (41.56%), Postives = 91/154 (59.09%), Query Frame = 0


Query: 5   SSPAKLSPSDDDSHNYDEFSIDDGTRDMTDEEMSEYVRAFKETEGFDMPSFPDTVAVSRI 64
           +S   LS  +++  + D+    DG   +T +E  EY RA +E+EGFD+P F    + S I
Sbjct: 2   ASSNHLSEEEEEEIDSDDEFYQDGYHTITKKEEREYFRAVEESEGFDVPDFDKVFSYSLI 61

Query: 65  ECITGH-RLYTEENISEGSKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKP 124
             I  H   +  E +   +  AI HYN++NGT+FE V+IVKAN+    G +YY+TF  KP
Sbjct: 62  APIDMHENSWMLEEVRISANKAIKHYNKENGTNFEVVEIVKANHSGCCGSMYYITFNVKP 121

Query: 125 RGTPRDFPTTTFQAWVLFFI---DEVEVQLCRPK 155
            GT  +FP  TFQA V + I   D ++V+LCRPK
Sbjct: 122 IGTSTEFPLITFQAKVYYAIPIKDIIDVELCRPK 155

[Close]

BLAST of Spg036540 vs. NCBI nr
Match: XP_022942206.1 (uncharacterized protein LOC111447330 [Cucurbita moschata] >XP_022942207.1 uncharacterized protein LOC111447330 [Cucurbita moschata])

HSP 1 Score: 113.2 bits (282), Expect = 2.0e-21
Identity = 69/158 (43.67%), Postives = 92/158 (58.23%), Query Frame = 0


Query: 5   SSPAKLSPSDDDSHNYDEFSIDDGTRDMTDEEMSEYVRAFKETEGFDMPSFPDTVAVSRI 64
           SS   L    ++  + DEF   DG   +T +E  EY  A +E++GFD+P F +  A S I
Sbjct: 3   SSSHILEEEKEEIDSDDEF-FQDGYHTITKKEEMEYYCAVEESQGFDVPDFGNVYAFSLI 62

Query: 65  ECITGHR---LYTEENISEGSKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEA 124
             +  H    +  E  IS     AI HYN +NGT+FE V IVKAN+    G +YY+TF  
Sbjct: 63  TPMKLHEVPGMLKEVQISANE--AIKHYNNENGTNFEVVDIVKANHSGCCGSMYYITFNV 122

Query: 125 KPRGTPRDFPTTTFQAWVLFFI---DEVEVQLCRPKPS 157
           KP GTP +FP+ TFQA V + I   D ++V+LCRPKPS
Sbjct: 123 KPIGTPLEFPSITFQAKVYYAIPIKDIIDVELCRPKPS 157

[Close]

BLAST of Spg036540 vs. ExPASy Swiss-Prot
Match: P37842 (Multicystatin OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.8e-04
Identity = 18/49 (36.73%), Postives = 31/49 (63.27%), Query Frame = 0


Query: 82  SKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKPRGTPRDF 131
           ++ A+  YN++N +S EF K++    Q   GI+YY+TFEA   G  +++
Sbjct: 22  ARFAVQDYNQKNDSSLEFKKVLNVKQQIVAGIMYYITFEATEGGNKKEY 70

[Close]

BLAST of Spg036540 vs. ExPASy TrEMBL
Match: A0A6J1C8H7 (uncharacterized protein LOC111008952 OS=Momordica charantia OX=3673 GN=LOC111008952 PE=4 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 5.1e-31
Identity = 86/167 (51.50%), Postives = 111/167 (66.47%), Query Frame = 0


Query: 1   MAACSSPAKLSPSDDDSHN-------YDEFSIDDGTRDMTDEEMSEYVRAFKETEGFDMP 60
           MA+ +SP +LS  DDD  +       +D+    +  R+MT EE  EY  A K+TEGFDMP
Sbjct: 1   MASSNSP-QLSDDDDDFGDEPREMTIHDDAYFGNEPREMTLEEEIEYYIALKKTEGFDMP 60

Query: 61  SFPDTVAVSRIECITGHRLYTEENISEGSK-IAINHYNRQNGTSFEFVKIVKANNQAARG 120
           SFPD+ A  RIE ITG RL++EE      K  A++++N+QNGTSFEFVK+VKA N+A  G
Sbjct: 61  SFPDSYACGRIETITGSRLHSEELQECADKDKAVDYFNQQNGTSFEFVKMVKATNKAVVG 120

Query: 121 ILYYLTFEAKPRGTPRDFPTTTFQAWVL--FFIDEVEVQLCRPKPST 158
           I+YYLTFE K  G+P + PT T QA VL    I   +V+LCRP+PST
Sbjct: 121 IMYYLTFEVKQMGSPPNSPTKTLQARVLRGLPIGHFDVELCRPEPST 166

[Close]

BLAST of Spg036540 vs. ExPASy TrEMBL
Match: A0A6J1CAH1 (uncharacterized protein LOC111008920 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008920 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.2e-27
Identity = 74/150 (49.33%), Postives = 103/150 (68.67%), Query Frame = 0


Query: 10  LSPSDDDSHNYD-EFSIDDGTRDMTDEEMSEYVRAFKETEGFDMPSFPDTVAVSRIECIT 69
           LS   ++++ YD +   DDG R+M +EE++ Y +A +E+EGFD+PSFP T A + I  + 
Sbjct: 33  LSVPANNNYLYDSDGYYDDGVREMNEEEVNRYYKAIQESEGFDVPSFPGTYAFAIISPLP 92

Query: 70  GHRLYTEENISEGSKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKPRGTPR 129
              L +EE + E +  AI HYN +NG SFEFVK++KAN+QAA G L++LTF+ K  G P 
Sbjct: 93  LINLISEE-VRECAGQAIKHYNNENGASFEFVKMLKANSQAALGELFFLTFQVKQTGAPP 152

Query: 130 DFPTTTFQAWVLFFI--DEVEVQLCRPKPS 157
           D PTTT QA VL  I  D+ +VQLCRP+P+
Sbjct: 153 DSPTTTLQARVLAGISPDDFDVQLCRPEPT 181

[Close]

BLAST of Spg036540 vs. ExPASy TrEMBL
Match: A0A6J1FN74 (uncharacterized protein LOC111447330 OS=Cucurbita moschata OX=3662 GN=LOC111447330 PE=4 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 9.7e-22
Identity = 69/158 (43.67%), Postives = 92/158 (58.23%), Query Frame = 0


Query: 5   SSPAKLSPSDDDSHNYDEFSIDDGTRDMTDEEMSEYVRAFKETEGFDMPSFPDTVAVSRI 64
           SS   L    ++  + DEF   DG   +T +E  EY  A +E++GFD+P F +  A S I
Sbjct: 3   SSSHILEEEKEEIDSDDEF-FQDGYHTITKKEEMEYYCAVEESQGFDVPDFGNVYAFSLI 62

Query: 65  ECITGHR---LYTEENISEGSKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEA 124
             +  H    +  E  IS     AI HYN +NGT+FE V IVKAN+    G +YY+TF  
Sbjct: 63  TPMKLHEVPGMLKEVQISANE--AIKHYNNENGTNFEVVDIVKANHSGCCGSMYYITFNV 122

Query: 125 KPRGTPRDFPTTTFQAWVLFFI---DEVEVQLCRPKPS 157
           KP GTP +FP+ TFQA V + I   D ++V+LCRPKPS
Sbjct: 123 KPIGTPLEFPSITFQAKVYYAIPIKDIIDVELCRPKPS 157

[Close]

BLAST of Spg036540 vs. ExPASy TrEMBL
Match: A0A6J1C7D4 (uncharacterized protein LOC111008920 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008920 PE=4 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 9.7e-22
Identity = 67/150 (44.67%), Postives = 93/150 (62.00%), Query Frame = 0


Query: 10  LSPSDDDSHNYD-EFSIDDGTRDMTDEEMSEYVRAFKETEGFDMPSFPDTVAVSRIECIT 69
           LS   ++++ YD +   DDG R+M +EE++ Y +A +E+EGFD+PSFP T A + I  + 
Sbjct: 33  LSVPANNNYLYDSDGYYDDGVREMNEEEVNRYYKAIQESEGFDVPSFPGTYAFAIISPLP 92

Query: 70  GHRLYTEENISEGSKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKPRGTPR 129
              L +EE                 G SFEFVK++KAN+QAA G L++LTF+ K  G P 
Sbjct: 93  LINLISEE-----------------GASFEFVKMLKANSQAALGELFFLTFQVKQTGAPP 152

Query: 130 DFPTTTFQAWVLFFI--DEVEVQLCRPKPS 157
           D PTTT QA VL  I  D+ +VQLCRP+P+
Sbjct: 153 DSPTTTLQARVLAGISPDDFDVQLCRPEPT 165

[Close]

BLAST of Spg036540 vs. ExPASy TrEMBL
Match: A0A6J1FVU0 (uncharacterized protein LOC111447329 OS=Cucurbita moschata OX=3662 GN=LOC111447329 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 3.7e-21
Identity = 65/151 (43.05%), Postives = 89/151 (58.94%), Query Frame = 0


Query: 10  LSPSDDDSHNYDEFSIDDGTRDMTDEEMSEYVRAFKETEGFDMPSFPDTVAVSRIECITG 69
           LS  +++  + D+    DG   +T +E  EY RA +E+EGFD+P F    + S I  I  
Sbjct: 75  LSEEEEEEIDSDDEFYQDGYHTITKKEEREYFRAVEESEGFDVPDFDKVFSCSFIIPIDM 134

Query: 70  HR---LYTEENISEGSKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKPRGT 129
           H    +  E  IS     AI HYN++NGT+FE V+IVKAN+    G +YY+TF  KP GT
Sbjct: 135 HENSWMLKEVRISANK--AIKHYNKENGTNFEVVEIVKANHSGCCGSMYYITFNVKPIGT 194

Query: 130 PRDFPTTTFQAWVLFFI---DEVEVQLCRPK 155
             +FP  TFQA V + I   D ++V+LCRPK
Sbjct: 195 SAEFPPITFQAKVYYVIPIKDIIDVKLCRPK 223

[Close]

BLAST of Spg036540 vs. TAIR 10
Match: AT2G37435.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 52.8 bits (125), Expect = 3.0e-07
Identity = 34/143 (23.78%), Postives = 66/143 (46.15%), Query Frame = 0


Query: 26  DDGTRDMTDEEMSEYVRAFKETEGFDMPSFPDTVAVSRIECITGHRLY------------ 85
           D+  +   +EE    V+  ++++GFD       +  ++  C+  +R              
Sbjct: 38  DEEPKISAEEEYYLMVKEVEDSKGFD-------IDFAKFRCVFNYRPVDLDDNELALEPE 97

Query: 86  -TEENISEGSKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKPRGTPRDFPT 145
            T E +    + ++ H+N  + T +EFV+ +KAN+  + G++Y++TFE K      D  +
Sbjct: 98  TTREFMDRLCRKSLEHFNEIHSTKYEFVRFIKANHHVSAGMMYFITFEGKLLSNDDD--S 157

Query: 146 TTFQAWVLFFIDEVEVQLCRPKP 156
             FQA + +     E+  C  KP
Sbjct: 158 KQFQAKLCYCGGTPEIISCELKP 171

[Close]

BLAST of Spg036540 vs. TAIR 10
Match: AT4G15715.1 (Proteinase inhibitor I25, cystatin, conserved region )

HSP 1 Score: 51.6 bits (122), Expect = 6.7e-07
Identity = 29/72 (40.28%), Postives = 40/72 (55.56%), Query Frame = 0


Query: 85  AINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKPRGTPRDFPTTTFQAWVLFFID 144
           AI+  N + GT  EFVK V AN    +G L+Y+TF AK   +P   P   +QA V  F  
Sbjct: 46  AIDEENEETGTRLEFVKYVSANVLGVQGFLFYITFWAKDLSSPNPQP-HCYQAKVRKFAA 105

Query: 145 EVEVQLCRPKPS 157
           E++V   R +P+
Sbjct: 106 EIDVSEFRLRPT 116

[Close]

BLAST of Spg036540 vs. TAIR 10
Match: AT1G63190.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 50.8 bits (120), Expect = 1.1e-06
Identity = 32/84 (38.10%), Postives = 45/84 (53.57%), Query Frame = 0


Query: 74  TEENISEGSKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKPRGTPRDFPTT 133
           T + +   S+ A++ YN+++ T FEFVK+VKAN      I++ +TFE      P D    
Sbjct: 91  TRDLLERLSRKALDDYNQESRTQFEFVKVVKANFHFCCAIMFLITFEVV---DPYDNLIK 150

Query: 134 TFQAWVLFFIDEV-EVQLCRPKPS 157
            FQ  V    D V E   CRPKP+
Sbjct: 151 LFQTRVRHAEDIVTEYVFCRPKPN 171

[Close]

BLAST of Spg036540 vs. TAIR 10
Match: AT1G50690.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 1.5e-06
Identity = 29/74 (39.19%), Postives = 38/74 (51.35%), Query Frame = 0


Query: 82  SKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKPRGTPRDFPTTTFQAWVLF 141
           SKIA+  YN     + E V+ VKAN +   G ++Y+TFEAK   +  D    TFQA V +
Sbjct: 98  SKIALQKYNDDKLENLELVRAVKANRKYGAGFIFYITFEAKDANSHTD--PITFQAAVRY 157

Query: 142 FIDEVEVQLCRPKP 156
                 V    PKP
Sbjct: 158 LRGIETVYRVHPKP 169

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BLAST of Spg036540 vs. TAIR 10
Match: AT1G63200.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 46.6 bits (109), Expect = 2.1e-05
Identity = 28/84 (33.33%), Postives = 45/84 (53.57%), Query Frame = 0


Query: 74  TEENISEGSKIAINHYNRQNGTSFEFVKIVKANNQAARGILYYLTFEAKPRGTPRDFPTT 133
           TE+ +   ++ A++ +N ++GT +EFVK+VKAN   A  +++ +TF+ K    P D    
Sbjct: 92  TEDLLKSLAQEALDDHNGRHGTEYEFVKVVKANFHFACAMMFLITFQVK---DPYDDMVK 151

Query: 134 TFQAWVLFFID-EVEVQLCRPKPS 157
            FQ  V            CRPKP+
Sbjct: 152 LFQTRVRHGKHITTNYVFCRPKPN 172

The following BLAST results are available for this feature:

BLAST of Spg036540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137526.1	1.1e-30	51.50	uncharacterized protein LOC111008952 [Momordica charantia] >XP_022137527.1 uncha...	[more]
XP_022137488.1	2.4e-27	49.33	uncharacterized protein LOC111008920 isoform X1 [Momordica charantia]	[more]
XP_023525925.1	1.6e-23	47.59	uncharacterized protein LOC111789396 [Cucurbita pepo subsp. pepo]	[more]
XP_023521681.1	1.5e-21	41.56	UPF0725 protein At4g29550-like [Cucurbita pepo subsp. pepo]	[more]
XP_022942206.1	2.0e-21	43.67	uncharacterized protein LOC111447330 [Cucurbita moschata] >XP_022942207.1 unchar...	[more]

BLAST of Spg036540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P37842	1.8e-04	36.73	Multicystatin OS=Solanum tuberosum OX=4113 PE=1 SV=1	[more]

BLAST of Spg036540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C8H7	5.1e-31	51.50	uncharacterized protein LOC111008952 OS=Momordica charantia OX=3673 GN=LOC111008...	[more]
A0A6J1CAH1	1.2e-27	49.33	uncharacterized protein LOC111008920 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1FN74	9.7e-22	43.67	uncharacterized protein LOC111447330 OS=Cucurbita moschata OX=3662 GN=LOC1114473...	[more]
A0A6J1C7D4	9.7e-22	44.67	uncharacterized protein LOC111008920 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1FVU0	3.7e-21	43.05	uncharacterized protein LOC111447329 OS=Cucurbita moschata OX=3662 GN=LOC1114473...	[more]

BLAST of Spg036540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G37435.1	3.0e-07	23.78	Cystatin/monellin superfamily protein	[more]
AT4G15715.1	6.7e-07	40.28	Proteinase inhibitor I25, cystatin, conserved region	[more]
AT1G63190.1	1.1e-06	38.10	Cystatin/monellin superfamily protein	[more]
AT1G50690.1	1.5e-06	39.19	Cystatin/monellin superfamily protein	[more]
AT1G63200.1	2.1e-05	33.33	Cystatin/monellin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF00031	Cystatin	coord: 76..122 e-value: 3.0E-7 score: 30.6
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 74..136 e-value: 3.33207E-6 score: 41.6908
IPR006525	Cystatin-related, plant	TIGRFAM	TIGR01638	TIGR01638	coord: 79..156 e-value: 3.4E-13 score: 47.5
None	No IPR available	GENE3D	3.10.450.10		coord: 69..147 e-value: 3.0E-12 score: 48.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..32
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 14..32
None	No IPR available	PANTHER	PTHR31260	CYSTATIN/MONELLIN SUPERFAMILY PROTEIN	coord: 15..156
None	No IPR available	PANTHER	PTHR31260:SF28	CYSTATIN/MONELLIN SUPERFAMILY PROTEIN	coord: 15..156
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 78..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg036540.1	Spg036540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity