Spg036527 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg036527
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionTransmembrane 9 superfamily member
Locationscaffold5: 48612844 .. 48617269 (+)
RNA-Seq ExpressionSpg036527
SyntenySpg036527
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGTTTAGGGAAAAGCAGGGCGTGGAAGCGTAAAAGGAAAGAAAGGGCGAAGAAGTGGCCTTACAAATGCAAACGTCCGGCACTGTAGGCGTAGGTGAGGCATCAAACATCTTTAAACGACGCCACATCTCTATCAGTCTCGAGCGTAGCTCAGGCTCAGGCTCAGGCCCAGCAAACAAACCCCAGCCTTAACCCAACCTCTCATCGACTTCCGACCTTAAAACGCCCTCTTCCTCTCATCATTTTTCTAAACCCCATATCGGATCCCATCCCTCTTCTTCCCATTTCCCTTTCTTCTCTGACCACTCCCTGCTTCTACCTCACTGCTTCCTTCACAATGGGAAGATTTGGGGCGGTGCTATTCATCGCCTTACTTGTTCTTCTCTGCGGATCTGTTCAGGTCAGATCTGATGGATCGGACCATCGCTATAAAGATGGAGATTCTGTTCCTCTCTATGCTAACAAAGTTGGCCCATTTCATAATCCGAGGTAATTCCACCTCCCTCTCATCAAATCTTCTCCTTCCTCTCCTTCAATCTCTTATAAGATCTACGGTTTTATCGCTGTTTTCTCCTCTGCTACTTTTTGATTCGTAGACTTGTAGCTGCTAGCTTTCATGGGATTGAATTACTTGTAGATTTCGTGATTTCAATCCAGATCTGAGCTAATTTGTGCAGTCAATGCGTTGACTGTAAAATGTCACTTCTGATCTGCTATATCTTTGAGCTAAATTACTGCATTTTTTTTTTTAAAGTTCGCCTACCGCTTAATACATGAGGAATATTCAAGACACTTGAGATACCGTCTTCAAGATATTCTGTTTTATTCCTTAACGAAGTGACAGCCTTACCGATTTTGTTTCGGTGGCGTGCCTTCTTGCTAGAATATCCGAATTTCCATCTTTCAGGCTAAAGATTAACTTCATTTTTTTGTTGATTCATATTGGGTTCATAGAAATAAGATTTTTACTCTAGACCCTAACTTATGAAACGTATCTGATATAGTTGCGTAGGCGTTTGAAGGTGTTGTAATTTGCTACCCCATATTTTCTATAACATATATTTACACTTGTTTTTTCTTTTGGTCACAGTGAAACTTACCGCTACTTCGATCTCCCATTCTGCGTACCAGGTATGTGTCTATTGTGCTAATTTCCCTGGGCAACCCCCCCCCCCCCCCCCCCCAAAAAAAAAAAAAAAAAGTTTCACATTGTAGAGCTTATTTGTTGGATGGAGAACCAATTAGATGAATCTAAATTGCAGATGATGTGAAGGAGAAAAAGGAGGCTCTTGGTGAAGTATTAAATGGAGATCGTTTAGTTAGTGCCCCATACAAACTTGACTTCTTGCAAGAGAAGGATTCTCTTTCTGTTTGCAAGAAAAAACTTTCAAAGGAAGACGTTGCTAGGTTTCGTGCTGCAGTAGACAAGGACTACTACTTTCAGATGTATTATGATGATTTGCCAATTTGGGGTTTCATAGGGAAGGTTGACAAGGAAGGAAAAGAACCAAGTGATTTTAAATATTACCTATACAAGCATATCCATTTTGATATCTTTTATAACAAGGATCGTGTTATTGAAATAAATGTCAGAACAGACCCCAATGCCCTTGTTGATCTCACAGAGGACAAGGAAGTTGATGTTGAATTTCTATACACTGTAAAATGGAAGGAGACAACCACCCCATTTGAGAACAGGATGGATAAGTACTCCCAATCATCGTCATTACCTCATCATTTGGAAATTCATTGGTTCTCAATTATAAACTCATGTGTAACTGTCCTCCTCCTTACTGGATTTCTTGCCACCATTCTTATGCGAGTACTTAAGAATGACTTTGTCAAGTAAGAAGATTTAGTATTCTTTTTTTACAGTGTTTGTTCTGCCTGTCATTTCAATTTGTGCCCTCTTCTAAGGGAGCTACTGTCTTTTTACGTTACGCTTTTGTGTCTTTGATATAGATATGCCCATGATGAGGAATCAGCTGAGGACCAGGAGGAGACCGGATGGAAATACATACATGGTGATGTGTTTAGGTACCCAAAGCACAAGTCTCTTTTCGCAGCCTGTCTTGGTTCTGGTACCCAACTGTTTACACTGTAAGTCATAACTCATCTTGTCAACCATCAAAACTAATATTTATAAACGTCTGGTTCCCTGTCACCAATATTTTTTATTGACTACGTTGTACATTTATCTCTACAGTACGGTGTTCATTTTCATACTTGCACTTGTTGGAGTGTTTTATCCATACAACCGAGGGGCATTATTTACTGCACTAGTTGTTATCTATGCTCTCACATCTGGTATTGCTGGCTATACTGCAACTTCCTTTTATTGCCAGCTCGAGGGAACAAACTGGGTACTTTCTCTTTGAATTATATTTCTTTCAATTACTTCAGTATGTTGGGCTAACAAGGTCTTCAACTTCTCAATGATAGGTTAGGAATCTACTGTTGACGGGATGCCTTTTCTGTGGGCCTCTGTTTCTGACATTTTGCTTTCTAAATACCGTTGCAATTGCCTATACAGCAACTGCTGCCCTTCCTTTTGGTACTATTGTTGTAATAGTTCTCATATGGACTCTTGTGACATCTCCTTTGCTGGTGTTGGGTGGGATTGCTGGTAAAAACAGCAAGGCTGAATTTCAAGCTCCAGTTCGCACCACCAAATATCCTAGAGAGATTCCACCTCTGCCTTGGTACCGTGGGACAATTCCACAGATGGCAATGGCTGGATTTCTGCCTTTCAGTGCCATATATATTGAGTTATACTACATATTTGCCAGTGTGTGGGGCCACCGGATTTACACAATATACAGCATCTTGTTTATTGTCTTCATTATTCTCTTGATAGTTACGGCATTCATTACTGTTGCATTAACATACTTCCAACTTGCTGCCGAGGACCATGAATGGTGGTGGAGGTATGCAACTGCTACCCCTTTGTAGCTAGCTAATGATTAATTGCATTTTTTTAGTAATTTTACAACTTTTATCATTAAGCTGTGTGTGTTTCCGTTTCATCCTTTTTCGTTCTTATAAATTTATAATACTGATTATTTGGAGAACATTCAGGTGGATATTATTTTTACTCATTTTTTTCTTCCACCAAAATCTCTGATGATATAGAGGTAGGCGATAAAGCCTGTTTTCACTAATTATGAAATGGTTTCTAGTTGAACAGGGCATTTATGGGAAATGAATTTGTTTACAAGGTAAAAGTTCTGAAAAGTTCATAATAGATATTCAGAATGTTTGAGTTGTTCCAAAAAAAAAAAAAAACCAGCATCGTGTTTTCTTCTTCTCCTTTTCTCCCCTTTCTTAGACATTACCCTTTTGGTAGCTTTTATTACTTTGAAAAAAAATTGGTTGCTTAAGCTTGCAAGAAAGGTGTTTCTATGAGGAAAATAATATTCTTCTGGCTGCTGAATATATGCTGTCAGATTTCTCGTAGTAGTCGTTAATGTGTCGGATCTAAATGTTTTGTTTTGTGCAACTGTTACTTTATGATTCTTTGTTTGGAATGCATTTCTCTTGGAGACCTCTTTACAGACGTCCGGTTTAACATCTATCCATGAATGCTAGAATATTTAGATTGCATATACGTGCTGGTATAAGCAATGTTGGATGAGATGGCAGTTTAAGTAGGTATTTGGATATCTTTGATATATTATAGAACTGTTGAAACGTGCTTTCAATATTCTTTTTCTAATATGTCTAGCCGTTCAAGCACTGTGGCTTGGAATATAAAGCCATGCTTAATTTGTTAAACTCCTTTGTCTTCATTCTTTTGCAGATCTTTTCTTTGCGGTGGGTCAACTGGCTTATTTATCTATGCGTATTGTTTGTACTACTACTACGCTCGCTCGGATATGTCTGGGTTTATGCAAACATCATTTTTCTTCGGCTACATGGCTTGTGTCTGCTATGGCTTCTTCCTGATGCTTGGAGCTATTGGTTTTCGAGCTGCTTTGCTTTTTGTCCGCCACATATATCGATCCATCAAGTGCGAGTAGTCCTGGTACCCATTACTCAATACTGAGAGACGAGTTATGTTTCTTTAACTTTTCATATTCATAGGTTTCTATGTAGAAAAGTTACCTGCAGTTCTTTTTTTGTCTTTTTGGTGATATGTCTTAAAAAGGGAACAGCAATTTCTTTAGATATTATGCTCTAAGAGCAGAGCAGCATGGTGGCAGGGTGGATGAACGTGCCAGGCACGAGCTTGGGGAACAGAGTTTGTACCATTACATTAGTCCCAACACTTTTGTTTTAATCTGAAGTTTGTCCAATACATTATTGTTCTTCCTCTCAAGTCTCAACGCCCTTTTATGATGGAATACTCATCGAGGAATTGAATTCATTTGGTCGGTCAATTGTTCCCTCAATTTTAATTCAAACCAGAAATAAGCTCCGGTTTCC

mRNA sequence

ATGGGAAGATTTGGGGCGGTGCTATTCATCGCCTTACTTGTTCTTCTCTGCGGATCTGTTCAGGTCAGATCTGATGGATCGGACCATCGCTATAAAGATGGAGATTCTGTTCCTCTCTATGCTAACAAAGTTGGCCCATTTCATAATCCGAGTGAAACTTACCGCTACTTCGATCTCCCATTCTGCGTACCAGATGATGTGAAGGAGAAAAAGGAGGCTCTTGGTGAAGTATTAAATGGAGATCGTTTAGTTAGTGCCCCATACAAACTTGACTTCTTGCAAGAGAAGGATTCTCTTTCTGTTTGCAAGAAAAAACTTTCAAAGGAAGACGTTGCTAGGTTTCGTGCTGCAGTAGACAAGGACTACTACTTTCAGATGTATTATGATGATTTGCCAATTTGGGGTTTCATAGGGAAGGTTGACAAGGAAGGAAAAGAACCAAGTGATTTTAAATATTACCTATACAAGCATATCCATTTTGATATCTTTTATAACAAGGATCGTGTTATTGAAATAAATGTCAGAACAGACCCCAATGCCCTTGTTGATCTCACAGAGGACAAGGAAGTTGATGTTGAATTTCTATACACTGTAAAATGGAAGGAGACAACCACCCCATTTGAGAACAGGATGGATAAGTACTCCCAATCATCGTCATTACCTCATCATTTGGAAATTCATTGGTTCTCAATTATAAACTCATGTGTAACTGTCCTCCTCCTTACTGGATTTCTTGCCACCATTCTTATGCGAGTACTTAAGAATGACTTTGTCAAATATGCCCATGATGAGGAATCAGCTGAGGACCAGGAGGAGACCGGATGGAAATACATACATGGTGATGTGTTTAGGTACCCAAAGCACAAGTCTCTTTTCGCAGCCTGTCTTGGTTCTGGTACCCAACTGTTTACACTTACGGTGTTCATTTTCATACTTGCACTTGTTGGAGTGTTTTATCCATACAACCGAGGGGCATTATTTACTGCACTAGTTGTTATCTATGCTCTCACATCTGGTATTGCTGGCTATACTGCAACTTCCTTTTATTGCCAGCTCGAGGGAACAAACTGGGTTAGGAATCTACTGTTGACGGGATGCCTTTTCTGTGGGCCTCTGTTTCTGACATTTTGCTTTCTAAATACCGTTGCAATTGCCTATACAGCAACTGCTGCCCTTCCTTTTGGTACTATTGTTGTAATAGTTCTCATATGGACTCTTGTGACATCTCCTTTGCTGGTGTTGGGTGGGATTGCTGGTAAAAACAGCAAGGCTGAATTTCAAGCTCCAGTTCGCACCACCAAATATCCTAGAGAGATTCCACCTCTGCCTTGGTACCGTGGGACAATTCCACAGATGGCAATGGCTGGATTTCTGCCTTTCAGTGCCATATATATTGAGTTATACTACATATTTGCCAGTGTGTGGGGCCACCGGATTTACACAATATACAGCATCTTGTTTATTGTCTTCATTATTCTCTTGATAGTTACGGCATTCATTACTGTTGCATTAACATACTTCCAACTTGCTGCCGAGGACCATGAATGGTGGTGGAGATCTTTTCTTTGCGGTGGGTCAACTGGCTTATTTATCTATGCGTATTGTTTGTACTACTACTACGCTCGCTCGGATATGTCTGGGTTTATGCAAACATCATTTTTCTTCGGCTACATGGCTTGTGTCTGCTATGGCTTCTTCCTGATGCTTGGAGCTATTGGTTTTCGAGCTGCTTTGCTTTTTGTCCGCCACATATATCGATCCATCAAGTGCGAGTAG

Coding sequence (CDS)

ATGGGAAGATTTGGGGCGGTGCTATTCATCGCCTTACTTGTTCTTCTCTGCGGATCTGTTCAGGTCAGATCTGATGGATCGGACCATCGCTATAAAGATGGAGATTCTGTTCCTCTCTATGCTAACAAAGTTGGCCCATTTCATAATCCGAGTGAAACTTACCGCTACTTCGATCTCCCATTCTGCGTACCAGATGATGTGAAGGAGAAAAAGGAGGCTCTTGGTGAAGTATTAAATGGAGATCGTTTAGTTAGTGCCCCATACAAACTTGACTTCTTGCAAGAGAAGGATTCTCTTTCTGTTTGCAAGAAAAAACTTTCAAAGGAAGACGTTGCTAGGTTTCGTGCTGCAGTAGACAAGGACTACTACTTTCAGATGTATTATGATGATTTGCCAATTTGGGGTTTCATAGGGAAGGTTGACAAGGAAGGAAAAGAACCAAGTGATTTTAAATATTACCTATACAAGCATATCCATTTTGATATCTTTTATAACAAGGATCGTGTTATTGAAATAAATGTCAGAACAGACCCCAATGCCCTTGTTGATCTCACAGAGGACAAGGAAGTTGATGTTGAATTTCTATACACTGTAAAATGGAAGGAGACAACCACCCCATTTGAGAACAGGATGGATAAGTACTCCCAATCATCGTCATTACCTCATCATTTGGAAATTCATTGGTTCTCAATTATAAACTCATGTGTAACTGTCCTCCTCCTTACTGGATTTCTTGCCACCATTCTTATGCGAGTACTTAAGAATGACTTTGTCAAATATGCCCATGATGAGGAATCAGCTGAGGACCAGGAGGAGACCGGATGGAAATACATACATGGTGATGTGTTTAGGTACCCAAAGCACAAGTCTCTTTTCGCAGCCTGTCTTGGTTCTGGTACCCAACTGTTTACACTTACGGTGTTCATTTTCATACTTGCACTTGTTGGAGTGTTTTATCCATACAACCGAGGGGCATTATTTACTGCACTAGTTGTTATCTATGCTCTCACATCTGGTATTGCTGGCTATACTGCAACTTCCTTTTATTGCCAGCTCGAGGGAACAAACTGGGTTAGGAATCTACTGTTGACGGGATGCCTTTTCTGTGGGCCTCTGTTTCTGACATTTTGCTTTCTAAATACCGTTGCAATTGCCTATACAGCAACTGCTGCCCTTCCTTTTGGTACTATTGTTGTAATAGTTCTCATATGGACTCTTGTGACATCTCCTTTGCTGGTGTTGGGTGGGATTGCTGGTAAAAACAGCAAGGCTGAATTTCAAGCTCCAGTTCGCACCACCAAATATCCTAGAGAGATTCCACCTCTGCCTTGGTACCGTGGGACAATTCCACAGATGGCAATGGCTGGATTTCTGCCTTTCAGTGCCATATATATTGAGTTATACTACATATTTGCCAGTGTGTGGGGCCACCGGATTTACACAATATACAGCATCTTGTTTATTGTCTTCATTATTCTCTTGATAGTTACGGCATTCATTACTGTTGCATTAACATACTTCCAACTTGCTGCCGAGGACCATGAATGGTGGTGGAGATCTTTTCTTTGCGGTGGGTCAACTGGCTTATTTATCTATGCGTATTGTTTGTACTACTACTACGCTCGCTCGGATATGTCTGGGTTTATGCAAACATCATTTTTCTTCGGCTACATGGCTTGTGTCTGCTATGGCTTCTTCCTGATGCTTGGAGCTATTGGTTTTCGAGCTGCTTTGCTTTTTGTCCGCCACATATATCGATCCATCAAGTGCGAGTAG

Protein sequence

MGRFGAVLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDKDYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNALVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLLLTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGTQLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRNLLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE
Homology
BLAST of Spg036527 vs. NCBI nr
Match: XP_038894421.1 (transmembrane 9 superfamily member 3 [Benincasa hispida])

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 583/591 (98.65%), Postives = 589/591 (99.66%), Query Frame = 0

Query: 1   MGRFGAVLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLP 60
           MGRFGAV+FIALLVLLCGSV VRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLP
Sbjct: 1   MGRFGAVVFIALLVLLCGSVPVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLP 60

Query: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDK 120
           FC+PDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDS++VCKKKLSKEDVARFR+AVDK
Sbjct: 61  FCIPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSVTVCKKKLSKEDVARFRSAVDK 120

Query: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180
           DYYFQMYYDDLPIWGFIGKVDKEGK+PSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA
Sbjct: 121 DYYFQMYYDDLPIWGFIGKVDKEGKDPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180

Query: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240
           LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL
Sbjct: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240

Query: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300
           LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT
Sbjct: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300

Query: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN 360
           QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGY ATSFYCQLEGTNWVRN
Sbjct: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYVATSFYCQLEGTNWVRN 360

Query: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420
           LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK
Sbjct: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420

Query: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480
           NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY
Sbjct: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480

Query: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540
           TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY
Sbjct: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540

Query: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE
Sbjct: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 591

BLAST of Spg036527 vs. NCBI nr
Match: XP_008461818.1 (PREDICTED: transmembrane 9 superfamily member 3 isoform X1 [Cucumis melo] >KAA0036038.1 transmembrane 9 superfamily member 3 isoform X1 [Cucumis melo var. makuwa] >TYJ98841.1 transmembrane 9 superfamily member 3 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 584/591 (98.82%), Postives = 586/591 (99.15%), Query Frame = 0

Query: 1   MGRFGAVLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLP 60
           MGRFGAV FIALLVLLCGSV VRSDGSDHRYKDGD VPLYANKVGPFHNPSETYRYFDLP
Sbjct: 1   MGRFGAVPFIALLVLLCGSVPVRSDGSDHRYKDGDPVPLYANKVGPFHNPSETYRYFDLP 60

Query: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDK 120
           FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDS SVC+KKLSKEDVARFRAAVDK
Sbjct: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSSSVCQKKLSKEDVARFRAAVDK 120

Query: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180
           DYYFQMYYDDLPIWGFIGKVDKEGK+PSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA
Sbjct: 121 DYYFQMYYDDLPIWGFIGKVDKEGKDPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180

Query: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240
           LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL
Sbjct: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240

Query: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300
           LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT
Sbjct: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300

Query: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN 360
           QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGY ATSFYCQLEGTNWVRN
Sbjct: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYVATSFYCQLEGTNWVRN 360

Query: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420
           LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK
Sbjct: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420

Query: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480
           NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY
Sbjct: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480

Query: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540
           TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY
Sbjct: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540

Query: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE
Sbjct: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 591

BLAST of Spg036527 vs. NCBI nr
Match: XP_022924223.1 (transmembrane 9 superfamily member 3-like [Cucurbita moschata] >XP_023519963.1 transmembrane 9 superfamily member 3-like [Cucurbita pepo subsp. pepo] >KAG6584477.1 Transmembrane 9 superfamily member 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020064.1 Transmembrane 9 superfamily member 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 581/591 (98.31%), Postives = 587/591 (99.32%), Query Frame = 0

Query: 1   MGRFGAVLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLP 60
           MGRFGAV+FIALLV LCGSVQVRSDGSDHRYKDGD VPLYANKVGPFHNPSETYRYFDLP
Sbjct: 1   MGRFGAVVFIALLVFLCGSVQVRSDGSDHRYKDGDPVPLYANKVGPFHNPSETYRYFDLP 60

Query: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDK 120
           FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKD+ SVC+KKLSKEDVA+FRAAVDK
Sbjct: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDATSVCQKKLSKEDVAKFRAAVDK 120

Query: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180
           DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRV+EINVRTDPNA
Sbjct: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVVEINVRTDPNA 180

Query: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240
           LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL
Sbjct: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240

Query: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300
           LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT
Sbjct: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300

Query: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN 360
           QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGY ATSFYCQLEGTNWVRN
Sbjct: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYIATSFYCQLEGTNWVRN 360

Query: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420
           LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK
Sbjct: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420

Query: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480
           NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY
Sbjct: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480

Query: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540
           TIYSILF+VFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY
Sbjct: 481 TIYSILFLVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540

Query: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE
Sbjct: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 591

BLAST of Spg036527 vs. NCBI nr
Match: XP_022137162.1 (transmembrane 9 superfamily member 3 [Momordica charantia])

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 580/591 (98.14%), Postives = 588/591 (99.49%), Query Frame = 0

Query: 1   MGRFGAVLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLP 60
           MGRFGAVLFIALLVLLCGSV+VRSDGSDHRYKDG+SVPLYANKVGPFHNPSETYRYFDLP
Sbjct: 1   MGRFGAVLFIALLVLLCGSVRVRSDGSDHRYKDGESVPLYANKVGPFHNPSETYRYFDLP 60

Query: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDK 120
           FC+PD VKEKKEALGEVLNGDRLVSAPYKLDFLQEK+S++VCKK LSKEDVARFRAAVDK
Sbjct: 61  FCMPDHVKEKKEALGEVLNGDRLVSAPYKLDFLQEKESVAVCKKTLSKEDVARFRAAVDK 120

Query: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180
           DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA
Sbjct: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180

Query: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240
           LVDLTEDKEVDVEFLYTVKWKET TPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL
Sbjct: 181 LVDLTEDKEVDVEFLYTVKWKETNTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240

Query: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300
           LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT
Sbjct: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300

Query: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN 360
           QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN
Sbjct: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN 360

Query: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420
           LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK
Sbjct: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420

Query: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480
           NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY
Sbjct: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480

Query: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540
           TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY
Sbjct: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540

Query: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           ARSDMSGFMQTSFFFGYMACVCYGFFLMLG++GFRAALLFVRHIYRSIKCE
Sbjct: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGSVGFRAALLFVRHIYRSIKCE 591

BLAST of Spg036527 vs. NCBI nr
Match: XP_023524630.1 (transmembrane 9 superfamily member 3 [Cucurbita pepo subsp. pepo] >KAG7037065.1 Transmembrane 9 superfamily member 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 580/591 (98.14%), Postives = 588/591 (99.49%), Query Frame = 0

Query: 1   MGRFGAVLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLP 60
           MGRFGAV+FIALLVL+CGSV+VRSDGSDHRYKDG+SVPLYANKVGPFHNPSETYRYFDLP
Sbjct: 1   MGRFGAVVFIALLVLVCGSVRVRSDGSDHRYKDGESVPLYANKVGPFHNPSETYRYFDLP 60

Query: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDK 120
           FCVPD VKEKKEALGEVLNGDRLVSAPYKLDFLQEKDS+SVCKKKLSKE+VA+FRAAVDK
Sbjct: 61  FCVPDHVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSVSVCKKKLSKEEVAKFRAAVDK 120

Query: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180
           DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA
Sbjct: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180

Query: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240
           LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL
Sbjct: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240

Query: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300
           LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT
Sbjct: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300

Query: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN 360
           QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGY ATSFYCQLEG+NWVRN
Sbjct: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYVATSFYCQLEGSNWVRN 360

Query: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420
           LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK
Sbjct: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420

Query: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480
           NSKAEFQAPVRTTKYPREIP LPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY
Sbjct: 421 NSKAEFQAPVRTTKYPREIPSLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480

Query: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540
           TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY
Sbjct: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540

Query: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE
Sbjct: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 591

BLAST of Spg036527 vs. ExPASy Swiss-Prot
Match: Q9ZPS7 (Transmembrane 9 superfamily member 3 OS=Arabidopsis thaliana OX=3702 GN=TMN3 PE=2 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 1.8e-304
Identity = 519/586 (88.57%), Postives = 551/586 (94.03%), Query Frame = 0

Query: 7   VLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCVPDD 66
           +LFI  L+   G+  VRSD SDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFC+P+ 
Sbjct: 8   LLFIGALI-FSGAGTVRSDASDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCIPEG 67

Query: 67  VKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDKDYYFQM 126
           VK+KKEALGEVLNGDRLVSAPYKL+F  EKDS   CKKKLS+E+V  FR AV+KDYYFQM
Sbjct: 68  VKDKKEALGEVLNGDRLVSAPYKLNFRDEKDSEVYCKKKLSREEVEHFRRAVEKDYYFQM 127

Query: 127 YYDDLPIWGFIGKVDKEGK-EPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNALVDLT 186
           YYDDLPIWGFIGKVDKE K +PS+FKY+LYKHI F+I YNKDRVIEIN R DP++LVDLT
Sbjct: 128 YYDDLPIWGFIGKVDKESKSDPSEFKYFLYKHIQFEILYNKDRVIEINARMDPHSLVDLT 187

Query: 187 EDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLLLTGFL 246
           EDKEVD EF+YTVKWKET T FE RMDKY+ SSSLPHHLEIHWFSIINSCVTVLLLTGFL
Sbjct: 188 EDKEVDAEFMYTVKWKETETSFEKRMDKYAMSSSLPHHLEIHWFSIINSCVTVLLLTGFL 247

Query: 247 ATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGTQLFTL 306
           ATILMRVLKNDF+KYA DEE+A+DQEETGWKYIHGDVFR+PK+KSLFAA LGSGTQLFTL
Sbjct: 248 ATILMRVLKNDFMKYAQDEEAADDQEETGWKYIHGDVFRFPKNKSLFAASLGSGTQLFTL 307

Query: 307 TVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRNLLLTG 366
           T+FIF+L+LVGVFYPYNRGALFTALVVIYALTSGIAGYTA+SFYCQLEG NWVRNLLLTG
Sbjct: 308 TIFIFMLSLVGVFYPYNRGALFTALVVIYALTSGIAGYTASSFYCQLEGKNWVRNLLLTG 367

Query: 367 CLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAE 426
            LFCGPLFLTFCFLNTVAIAY+ATAALPFGTI+VIVLIWTLVTSPLLVLGGIAGKNSKAE
Sbjct: 368 GLFCGPLFLTFCFLNTVAIAYSATAALPFGTIIVIVLIWTLVTSPLLVLGGIAGKNSKAE 427

Query: 427 FQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSI 486
           FQAPVRTTKYPREIPPLPWYR  +PQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSI
Sbjct: 428 FQAPVRTTKYPREIPPLPWYRSAVPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSI 487

Query: 487 LFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDM 546
           LFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDM
Sbjct: 488 LFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDM 547

Query: 547 SGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           SGFMQTSFFFGYMAC+CYGFFLMLG +GFRAALLFVRHIYRSIKCE
Sbjct: 548 SGFMQTSFFFGYMACICYGFFLMLGTVGFRAALLFVRHIYRSIKCE 592

BLAST of Spg036527 vs. ExPASy Swiss-Prot
Match: Q940S0 (Transmembrane 9 superfamily member 2 OS=Arabidopsis thaliana OX=3702 GN=TMN2 PE=2 SV=1)

HSP 1 Score: 1038.9 bits (2685), Expect = 2.2e-302
Identity = 512/586 (87.37%), Postives = 547/586 (93.34%), Query Frame = 0

Query: 7   VLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCVPDD 66
           +L +   +L  G+  VRSD SDHRYK+GD+VPLYANKVGPFHNPSETYRYFDLPFC+P+ 
Sbjct: 7   ILLLVGAILFSGAGYVRSDASDHRYKEGDTVPLYANKVGPFHNPSETYRYFDLPFCIPEG 66

Query: 67  VKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDKDYYFQM 126
           VKEKKEALGEVLNGDRLVSAPYKL+F  EK+S   C KKLSKE+V +FR AV+KDYYFQM
Sbjct: 67  VKEKKEALGEVLNGDRLVSAPYKLNFRDEKESEVYCNKKLSKEEVKQFRKAVEKDYYFQM 126

Query: 127 YYDDLPIWGFIGKVDKEGK-EPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNALVDLT 186
           YYDDLPIWGFIGKVDK+ K +PS+FKY+LYKHI F+I YNKDRVIEI+ R DP++LVDLT
Sbjct: 127 YYDDLPIWGFIGKVDKDIKSDPSEFKYFLYKHIQFEILYNKDRVIEISARMDPHSLVDLT 186

Query: 187 EDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLLLTGFL 246
           EDKEVD EF+YTVKWKET TPFE RM+KYS SSSLPHHLEIHWFSIINSCVTVLLLTGFL
Sbjct: 187 EDKEVDAEFMYTVKWKETETPFEKRMEKYSMSSSLPHHLEIHWFSIINSCVTVLLLTGFL 246

Query: 247 ATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGTQLFTL 306
           ATILMRVLKNDF+KYA DEE+A+DQEETGWKYIHGDVFR+P H SLFAA LGSGTQLFTL
Sbjct: 247 ATILMRVLKNDFMKYAQDEEAADDQEETGWKYIHGDVFRFPTHNSLFAASLGSGTQLFTL 306

Query: 307 TVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRNLLLTG 366
           T+FIF+LALVGVFYPYNRGALFTALVVIYALTSGIAGYT+ SFYCQLEG +WVRNLLLTG
Sbjct: 307 TIFIFMLALVGVFYPYNRGALFTALVVIYALTSGIAGYTSASFYCQLEGKSWVRNLLLTG 366

Query: 367 CLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAE 426
           CLFCGPLFLTFCFLNTVAI YTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAE
Sbjct: 367 CLFCGPLFLTFCFLNTVAITYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAE 426

Query: 427 FQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSI 486
           FQAP RTTKYPREIPPLPWYR  IPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSI
Sbjct: 427 FQAPCRTTKYPREIPPLPWYRSAIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSI 486

Query: 487 LFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDM 546
           LFIVFIIL+IVTAFITVALTYFQLAAEDH+WWWRSFLCGGSTGLFIYAYCLYYYYARSDM
Sbjct: 487 LFIVFIILIIVTAFITVALTYFQLAAEDHQWWWRSFLCGGSTGLFIYAYCLYYYYARSDM 546

Query: 547 SGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           SGFMQTSFFFGYMAC+CYGFFLMLG +GFRAALLFVRHIYRSIKCE
Sbjct: 547 SGFMQTSFFFGYMACICYGFFLMLGTVGFRAALLFVRHIYRSIKCE 592

BLAST of Spg036527 vs. ExPASy Swiss-Prot
Match: Q9FHT4 (Transmembrane 9 superfamily member 4 OS=Arabidopsis thaliana OX=3702 GN=TMN4 PE=2 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 2.0e-287
Identity = 492/583 (84.39%), Postives = 529/583 (90.74%), Query Frame = 0

Query: 10  IALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCVPDDVKE 69
           + + + L G   V SDGSDHRYK GD VPLYANKVGPFHNPSETYRYFDLPFC    VKE
Sbjct: 11  LLVFLFLYGVSPVISDGSDHRYKVGDDVPLYANKVGPFHNPSETYRYFDLPFCSSAPVKE 70

Query: 70  KKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDKDYYFQMYYD 129
           KKEALGEVLNGDRLVSAPYKL+FL EK+S   C+K+LS+EDVA+FR  + KDYYFQMYYD
Sbjct: 71  KKEALGEVLNGDRLVSAPYKLEFLGEKNSEVACRKRLSREDVAKFRDVIAKDYYFQMYYD 130

Query: 130 DLPIWGFIGKVDKEGK-EPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNALVDLTEDK 189
           DLPIWGF+GKV KEGK +PS++KYYL+ H+ F+IFYNKDRVIEI VRTD N LVDLTEDK
Sbjct: 131 DLPIWGFLGKVVKEGKTDPSEYKYYLFNHLQFEIFYNKDRVIEIIVRTDQNFLVDLTEDK 190

Query: 190 EVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLLLTGFLATI 249
           EV V+F YTV+WKET  PFE RM+KYS +SS+PHHLEIHWFSIINSCVTVLLLTGFLATI
Sbjct: 191 EVQVDFTYTVRWKETEIPFEKRMEKYSLASSMPHHLEIHWFSIINSCVTVLLLTGFLATI 250

Query: 250 LMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGTQLFTLTVF 309
           LMRVLKNDFVKYAHDEE+ +DQEETGWK IHGDVFR+PKHKSL AA LGSGTQLFTL VF
Sbjct: 251 LMRVLKNDFVKYAHDEEAVDDQEETGWKLIHGDVFRFPKHKSLLAAALGSGTQLFTLAVF 310

Query: 310 IFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRNLLLTGCLF 369
           IF+LALVGVFYPYNRGALFTALVVIYALTSGIAGYTA SFYCQLEGTNWVRN++LTG LF
Sbjct: 311 IFMLALVGVFYPYNRGALFTALVVIYALTSGIAGYTAASFYCQLEGTNWVRNVILTGSLF 370

Query: 370 CGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAEFQA 429
           CGPL +TF FLNTVAIAY ATAALPFGTIVVI LIW LVTSPLL+LGGIAGKN K+EFQA
Sbjct: 371 CGPLLITFSFLNTVAIAYQATAALPFGTIVVIFLIWALVTSPLLILGGIAGKNRKSEFQA 430

Query: 430 PVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILFI 489
           P RTTKYPREIPP+ WYR T+PQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSIL I
Sbjct: 431 PCRTTKYPREIPPMRWYRRTLPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILSI 490

Query: 490 VFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDMSGF 549
           VF+IL+IVTAFITVALTYFQLAAEDHEWWWRS LCGGSTGLFIYAYCLYYYYARSDMSGF
Sbjct: 491 VFLILVIVTAFITVALTYFQLAAEDHEWWWRSLLCGGSTGLFIYAYCLYYYYARSDMSGF 550

Query: 550 MQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           MQTSFFFGYMAC+CYGFFLMLG IGF A+LLFVRHIYRSIKCE
Sbjct: 551 MQTSFFFGYMACICYGFFLMLGTIGFCASLLFVRHIYRSIKCE 593

BLAST of Spg036527 vs. ExPASy Swiss-Prot
Match: F4HW17 (Transmembrane 9 superfamily member 5 OS=Arabidopsis thaliana OX=3702 GN=TMN5 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 1.2e-165
Identity = 293/569 (51.49%), Postives = 402/569 (70.65%), Query Frame = 0

Query: 26  GSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCVPDDVKEKKEALGEVLNGDRLVS 85
           GS + Y  GD VPL+ NKVGP HNPSETY+Y+DLPFC    V EK+E LGEVLNGDRL+S
Sbjct: 24  GSSNHYNAGDHVPLFVNKVGPLHNPSETYQYYDLPFCRRGPVIEKQETLGEVLNGDRLMS 83

Query: 86  APYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDKDYYFQMYYDDLPIWGFIGKVDKE-- 145
           + YKL F ++K    +C+K+L+  D+ARFR  + +DYYFQMYYDDLP+WGF+GKV+ +  
Sbjct: 84  SLYKLKFREDKTHFVLCRKRLTSSDIARFRDIIAQDYYFQMYYDDLPLWGFVGKVEGDYF 143

Query: 146 GKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNALVDLTEDKEVDVEFLYTVKWKET 205
           G+     KYY++ H+ F++ YN D+VIEIN  +DP+ +VD++E+ E+DV+F Y+V W  T
Sbjct: 144 GQGEKHTKYYIFSHLKFNVLYNADKVIEINSFSDPSYMVDISENTEIDVQFTYSVSWNLT 203

Query: 206 TTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLLLTGFLATILMRVLKNDFVKYAHD 265
           +   E RM+KYS++S  P   +IH+FS +NS   V+LL G ++ + MR LKN+   Y+  
Sbjct: 204 SERSETRMNKYSRASFHPISQKIHFFSFLNSITVVVLLIGLISFLFMRHLKNELRSYSIG 263

Query: 266 EESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGTQLFTLTVFIFILALVGVFYPYNR 325
           +E  E+++E GWK +H DVFR P++ S   A LG+GTQL  L + +F LA  G  YPYNR
Sbjct: 264 DE--EERKEAGWKLVHSDVFRCPRNISWLCAILGTGTQLLILIIALFALAFTGFLYPYNR 323

Query: 326 GALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRNLLLTGCLFCGPLFLTFCFLNTVA 385
           G L T+LV++Y LTS +AGYT+TSF+ Q EG    R++ L G L+  P F+    LNTVA
Sbjct: 324 GMLLTSLVIMYTLTSIVAGYTSTSFHSQFEGNKQKRSVRLAGILYPVPFFIILSVLNTVA 383

Query: 386 IAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKN-SKAEFQAPVRTTKYPREIPPL 445
           I Y ATAALPFGTIV+I+LI+TL+  P L+LGG+ G      EFQ P    + PREIPP 
Sbjct: 384 ITYGATAALPFGTIVIIILIFTLLNIPFLMLGGVLGNRFGLLEFQPPSAVKRNPREIPPQ 443

Query: 446 PWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILLIVTAFITV 505
            WYR  + Q+ + GF+PFSA+ +E + ++AS+WG +IYT   I+   FI+L+ +++ + +
Sbjct: 444 NWYRRKLYQVFLGGFVPFSAVVLEWHQLYASLWGFKIYTSPGIMLFTFIVLIFLSSSVGI 503

Query: 506 ALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDMSGFMQTSFFFGYMACVC 565
            LTY QL+ EDHEWWWRS LCGG T +F+Y Y +  +Y RSDM+GF+Q SF+ GY A +C
Sbjct: 504 ILTYIQLSGEDHEWWWRSILCGGFTAVFMYGYGV-LFYLRSDMTGFLQLSFYLGYTALLC 563

Query: 566 YGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           Y  FL+LG I F A+L+F+RHIYRS+K E
Sbjct: 564 YALFLVLGTISFLASLMFIRHIYRSVKLE 589

BLAST of Spg036527 vs. ExPASy Swiss-Prot
Match: Q54ZW0 (Putative phagocytic receptor 1b OS=Dictyostelium discoideum OX=44689 GN=phg1b PE=2 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 3.4e-157
Identity = 273/587 (46.51%), Postives = 394/587 (67.12%), Query Frame = 0

Query: 7   VLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCVPDD 66
           +L I L+ ++  S+ +    + H +K+ D VP Y N VGP+ NP+ETY ++ LPFC P  
Sbjct: 5   ILLIYLICIIVSSIVLVESSNKHHFKENDEVPFYVNNVGPYSNPTETYEFYTLPFCKPSS 64

Query: 67  VKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDKDYYFQM 126
           +  KK  LGE+L GD  V + Y+  F    ++  +C+  L KED+ +F+ A+ + YY +M
Sbjct: 65  ISYKKTKLGEILQGDSAVLSDYQFPFKSSFENKQLCEYTLKKEDIEKFKKAIGEYYYAEM 124

Query: 127 YYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNALVDLTE 186
            YDDLPI+ F+G VD    + ++ +YYLY HI F+  YN D+VI +N+ T+   +++L++
Sbjct: 125 IYDDLPIFSFVGTVD--DSDLTNIRYYLYNHIPFEFDYNGDQVIRVNIDTEHIKVIELSD 184

Query: 187 DKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLLLTGFLA 246
             E+ ++  Y+ KW+ T   F  RMD Y +       LEIHW S++NS   V+LLT FLA
Sbjct: 185 QDEITLKLTYSAKWQPTEHEFSKRMDLYEE--FFTKELEIHWLSVMNSFFLVVLLTAFLA 244

Query: 247 TILMRVLKNDFVKYA--HDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGTQLFT 306
            ++M++LKND+ +Y+   +EE ++ QE+ GWK +HGDVFR+P +K++F+A  G G Q  +
Sbjct: 245 IMIMKILKNDYSRYSKTDEEEDSDYQEDYGWKLVHGDVFRFPPYKNVFSAFYGIGWQFIS 304

Query: 307 LTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRNLLLT 366
           +   I  L+L G+FYP N G ++TA +V+YALTSGI+GY +   Y  + G  W  N++LT
Sbjct: 305 IVCGILALSLFGMFYPNNGGNMYTAGIVLYALTSGISGYQSAKMYKNMGGNKWAWNIVLT 364

Query: 367 GCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKA 426
             LF  PLF+     NTVAI + +T ALP  T++ ++ IW  V  PL V+GGIAG+    
Sbjct: 365 ATLFVAPLFIVVILSNTVAITWHSTVALPILTMIEVITIWLFVGFPLTVVGGIAGRRLSE 424

Query: 427 EFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYS 486
            F+AP RT  +PRE+PP+ WYR    Q+ +AGFLPFSAIYIEL+YIF SVWGH  YT+Y 
Sbjct: 425 NFEAPCRTKNFPREVPPIQWYRRLPCQILIAGFLPFSAIYIELFYIFNSVWGHSTYTLYG 484

Query: 487 ILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSD 546
           IL +VF+IL+ VT  ITVALTYFQL+ EDH+WWW SF+ GGST +FIY Y +YYYY  S 
Sbjct: 485 ILCLVFLILINVTVCITVALTYFQLSMEDHKWWWNSFINGGSTVVFIYMYSIYYYYYISH 544

Query: 547 MSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           M G +Q +F+F YM  VC+ FF++LG +GF ++L+FV+ IYR++K +
Sbjct: 545 MYGLLQATFYFTYMLIVCFFFFILLGTVGFYSSLIFVKRIYRNLKSD 587

BLAST of Spg036527 vs. ExPASy TrEMBL
Match: A0A5A7SZM3 (Transmembrane 9 superfamily member OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00330 PE=3 SV=1)

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 584/591 (98.82%), Postives = 586/591 (99.15%), Query Frame = 0

Query: 1   MGRFGAVLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLP 60
           MGRFGAV FIALLVLLCGSV VRSDGSDHRYKDGD VPLYANKVGPFHNPSETYRYFDLP
Sbjct: 1   MGRFGAVPFIALLVLLCGSVPVRSDGSDHRYKDGDPVPLYANKVGPFHNPSETYRYFDLP 60

Query: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDK 120
           FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDS SVC+KKLSKEDVARFRAAVDK
Sbjct: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSSSVCQKKLSKEDVARFRAAVDK 120

Query: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180
           DYYFQMYYDDLPIWGFIGKVDKEGK+PSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA
Sbjct: 121 DYYFQMYYDDLPIWGFIGKVDKEGKDPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180

Query: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240
           LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL
Sbjct: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240

Query: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300
           LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT
Sbjct: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300

Query: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN 360
           QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGY ATSFYCQLEGTNWVRN
Sbjct: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYVATSFYCQLEGTNWVRN 360

Query: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420
           LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK
Sbjct: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420

Query: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480
           NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY
Sbjct: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480

Query: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540
           TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY
Sbjct: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540

Query: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE
Sbjct: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 591

BLAST of Spg036527 vs. ExPASy TrEMBL
Match: A0A6J1EBS7 (Transmembrane 9 superfamily member OS=Cucurbita moschata OX=3662 GN=LOC111431735 PE=3 SV=1)

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 581/591 (98.31%), Postives = 587/591 (99.32%), Query Frame = 0

Query: 1   MGRFGAVLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLP 60
           MGRFGAV+FIALLV LCGSVQVRSDGSDHRYKDGD VPLYANKVGPFHNPSETYRYFDLP
Sbjct: 1   MGRFGAVVFIALLVFLCGSVQVRSDGSDHRYKDGDPVPLYANKVGPFHNPSETYRYFDLP 60

Query: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDK 120
           FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKD+ SVC+KKLSKEDVA+FRAAVDK
Sbjct: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDATSVCQKKLSKEDVAKFRAAVDK 120

Query: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180
           DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRV+EINVRTDPNA
Sbjct: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVVEINVRTDPNA 180

Query: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240
           LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL
Sbjct: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240

Query: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300
           LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT
Sbjct: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300

Query: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN 360
           QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGY ATSFYCQLEGTNWVRN
Sbjct: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYIATSFYCQLEGTNWVRN 360

Query: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420
           LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK
Sbjct: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420

Query: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480
           NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY
Sbjct: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480

Query: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540
           TIYSILF+VFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY
Sbjct: 481 TIYSILFLVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540

Query: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE
Sbjct: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 591

BLAST of Spg036527 vs. ExPASy TrEMBL
Match: A0A1S3CG21 (Transmembrane 9 superfamily member OS=Cucumis melo OX=3656 GN=LOC103500066 PE=3 SV=1)

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 584/591 (98.82%), Postives = 586/591 (99.15%), Query Frame = 0

Query: 1   MGRFGAVLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLP 60
           MGRFGAV FIALLVLLCGSV VRSDGSDHRYKDGD VPLYANKVGPFHNPSETYRYFDLP
Sbjct: 1   MGRFGAVPFIALLVLLCGSVPVRSDGSDHRYKDGDPVPLYANKVGPFHNPSETYRYFDLP 60

Query: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDK 120
           FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDS SVC+KKLSKEDVARFRAAVDK
Sbjct: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSSSVCQKKLSKEDVARFRAAVDK 120

Query: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180
           DYYFQMYYDDLPIWGFIGKVDKEGK+PSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA
Sbjct: 121 DYYFQMYYDDLPIWGFIGKVDKEGKDPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180

Query: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240
           LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL
Sbjct: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240

Query: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300
           LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT
Sbjct: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300

Query: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN 360
           QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGY ATSFYCQLEGTNWVRN
Sbjct: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYVATSFYCQLEGTNWVRN 360

Query: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420
           LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK
Sbjct: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420

Query: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480
           NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY
Sbjct: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480

Query: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540
           TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY
Sbjct: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540

Query: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE
Sbjct: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 591

BLAST of Spg036527 vs. ExPASy TrEMBL
Match: A0A6J1C7H6 (Transmembrane 9 superfamily member OS=Momordica charantia OX=3673 GN=LOC111008705 PE=3 SV=1)

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 580/591 (98.14%), Postives = 588/591 (99.49%), Query Frame = 0

Query: 1   MGRFGAVLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLP 60
           MGRFGAVLFIALLVLLCGSV+VRSDGSDHRYKDG+SVPLYANKVGPFHNPSETYRYFDLP
Sbjct: 1   MGRFGAVLFIALLVLLCGSVRVRSDGSDHRYKDGESVPLYANKVGPFHNPSETYRYFDLP 60

Query: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDK 120
           FC+PD VKEKKEALGEVLNGDRLVSAPYKLDFLQEK+S++VCKK LSKEDVARFRAAVDK
Sbjct: 61  FCMPDHVKEKKEALGEVLNGDRLVSAPYKLDFLQEKESVAVCKKTLSKEDVARFRAAVDK 120

Query: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180
           DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA
Sbjct: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180

Query: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240
           LVDLTEDKEVDVEFLYTVKWKET TPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL
Sbjct: 181 LVDLTEDKEVDVEFLYTVKWKETNTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240

Query: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300
           LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT
Sbjct: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300

Query: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN 360
           QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN
Sbjct: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN 360

Query: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420
           LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK
Sbjct: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420

Query: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480
           NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY
Sbjct: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480

Query: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540
           TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY
Sbjct: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540

Query: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           ARSDMSGFMQTSFFFGYMACVCYGFFLMLG++GFRAALLFVRHIYRSIKCE
Sbjct: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGSVGFRAALLFVRHIYRSIKCE 591

BLAST of Spg036527 vs. ExPASy TrEMBL
Match: A0A6J1KLE4 (Transmembrane 9 superfamily member OS=Cucurbita maxima OX=3661 GN=LOC111495631 PE=3 SV=1)

HSP 1 Score: 1166.0 bits (3015), Expect = 0.0e+00
Identity = 578/591 (97.80%), Postives = 585/591 (98.98%), Query Frame = 0

Query: 1   MGRFGAVLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLP 60
           MGRFGAV+FIALL+ LCGSVQVRSD SDHRYKDGD VPLYANKVGPFHNPSETYRYFDLP
Sbjct: 1   MGRFGAVVFIALLIFLCGSVQVRSDASDHRYKDGDPVPLYANKVGPFHNPSETYRYFDLP 60

Query: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDK 120
           FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKD+ SVC+KKLSKE VA+FRAAVDK
Sbjct: 61  FCVPDDVKEKKEALGEVLNGDRLVSAPYKLDFLQEKDATSVCQKKLSKEGVAKFRAAVDK 120

Query: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNA 180
           DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRV+EINVRTDPNA
Sbjct: 121 DYYFQMYYDDLPIWGFIGKVDKEGKEPSDFKYYLYKHIHFDIFYNKDRVVEINVRTDPNA 180

Query: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240
           LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL
Sbjct: 181 LVDLTEDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLL 240

Query: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300
           LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT
Sbjct: 241 LTGFLATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGT 300

Query: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRN 360
           QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGY ATSFYCQLEGTNWVRN
Sbjct: 301 QLFTLTVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYIATSFYCQLEGTNWVRN 360

Query: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420
           LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK
Sbjct: 361 LLLTGCLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGK 420

Query: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480
           NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY
Sbjct: 421 NSKAEFQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIY 480

Query: 481 TIYSILFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540
           TIYSILF+VFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY
Sbjct: 481 TIYSILFLVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYY 540

Query: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE
Sbjct: 541 ARSDMSGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 591

BLAST of Spg036527 vs. TAIR 10
Match: AT2G01970.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 1045.8 bits (2703), Expect = 1.3e-305
Identity = 519/586 (88.57%), Postives = 551/586 (94.03%), Query Frame = 0

Query: 7   VLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCVPDD 66
           +LFI  L+   G+  VRSD SDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFC+P+ 
Sbjct: 8   LLFIGALI-FSGAGTVRSDASDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCIPEG 67

Query: 67  VKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDKDYYFQM 126
           VK+KKEALGEVLNGDRLVSAPYKL+F  EKDS   CKKKLS+E+V  FR AV+KDYYFQM
Sbjct: 68  VKDKKEALGEVLNGDRLVSAPYKLNFRDEKDSEVYCKKKLSREEVEHFRRAVEKDYYFQM 127

Query: 127 YYDDLPIWGFIGKVDKEGK-EPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNALVDLT 186
           YYDDLPIWGFIGKVDKE K +PS+FKY+LYKHI F+I YNKDRVIEIN R DP++LVDLT
Sbjct: 128 YYDDLPIWGFIGKVDKESKSDPSEFKYFLYKHIQFEILYNKDRVIEINARMDPHSLVDLT 187

Query: 187 EDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLLLTGFL 246
           EDKEVD EF+YTVKWKET T FE RMDKY+ SSSLPHHLEIHWFSIINSCVTVLLLTGFL
Sbjct: 188 EDKEVDAEFMYTVKWKETETSFEKRMDKYAMSSSLPHHLEIHWFSIINSCVTVLLLTGFL 247

Query: 247 ATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGTQLFTL 306
           ATILMRVLKNDF+KYA DEE+A+DQEETGWKYIHGDVFR+PK+KSLFAA LGSGTQLFTL
Sbjct: 248 ATILMRVLKNDFMKYAQDEEAADDQEETGWKYIHGDVFRFPKNKSLFAASLGSGTQLFTL 307

Query: 307 TVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRNLLLTG 366
           T+FIF+L+LVGVFYPYNRGALFTALVVIYALTSGIAGYTA+SFYCQLEG NWVRNLLLTG
Sbjct: 308 TIFIFMLSLVGVFYPYNRGALFTALVVIYALTSGIAGYTASSFYCQLEGKNWVRNLLLTG 367

Query: 367 CLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAE 426
            LFCGPLFLTFCFLNTVAIAY+ATAALPFGTI+VIVLIWTLVTSPLLVLGGIAGKNSKAE
Sbjct: 368 GLFCGPLFLTFCFLNTVAIAYSATAALPFGTIIVIVLIWTLVTSPLLVLGGIAGKNSKAE 427

Query: 427 FQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSI 486
           FQAPVRTTKYPREIPPLPWYR  +PQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSI
Sbjct: 428 FQAPVRTTKYPREIPPLPWYRSAVPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSI 487

Query: 487 LFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDM 546
           LFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDM
Sbjct: 488 LFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDM 547

Query: 547 SGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           SGFMQTSFFFGYMAC+CYGFFLMLG +GFRAALLFVRHIYRSIKCE
Sbjct: 548 SGFMQTSFFFGYMACICYGFFLMLGTVGFRAALLFVRHIYRSIKCE 592

BLAST of Spg036527 vs. TAIR 10
Match: AT1G14670.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 1038.9 bits (2685), Expect = 1.6e-303
Identity = 512/586 (87.37%), Postives = 547/586 (93.34%), Query Frame = 0

Query: 7   VLFIALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCVPDD 66
           +L +   +L  G+  VRSD SDHRYK+GD+VPLYANKVGPFHNPSETYRYFDLPFC+P+ 
Sbjct: 7   ILLLVGAILFSGAGYVRSDASDHRYKEGDTVPLYANKVGPFHNPSETYRYFDLPFCIPEG 66

Query: 67  VKEKKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDKDYYFQM 126
           VKEKKEALGEVLNGDRLVSAPYKL+F  EK+S   C KKLSKE+V +FR AV+KDYYFQM
Sbjct: 67  VKEKKEALGEVLNGDRLVSAPYKLNFRDEKESEVYCNKKLSKEEVKQFRKAVEKDYYFQM 126

Query: 127 YYDDLPIWGFIGKVDKEGK-EPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNALVDLT 186
           YYDDLPIWGFIGKVDK+ K +PS+FKY+LYKHI F+I YNKDRVIEI+ R DP++LVDLT
Sbjct: 127 YYDDLPIWGFIGKVDKDIKSDPSEFKYFLYKHIQFEILYNKDRVIEISARMDPHSLVDLT 186

Query: 187 EDKEVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLLLTGFL 246
           EDKEVD EF+YTVKWKET TPFE RM+KYS SSSLPHHLEIHWFSIINSCVTVLLLTGFL
Sbjct: 187 EDKEVDAEFMYTVKWKETETPFEKRMEKYSMSSSLPHHLEIHWFSIINSCVTVLLLTGFL 246

Query: 247 ATILMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGTQLFTL 306
           ATILMRVLKNDF+KYA DEE+A+DQEETGWKYIHGDVFR+P H SLFAA LGSGTQLFTL
Sbjct: 247 ATILMRVLKNDFMKYAQDEEAADDQEETGWKYIHGDVFRFPTHNSLFAASLGSGTQLFTL 306

Query: 307 TVFIFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRNLLLTG 366
           T+FIF+LALVGVFYPYNRGALFTALVVIYALTSGIAGYT+ SFYCQLEG +WVRNLLLTG
Sbjct: 307 TIFIFMLALVGVFYPYNRGALFTALVVIYALTSGIAGYTSASFYCQLEGKSWVRNLLLTG 366

Query: 367 CLFCGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAE 426
           CLFCGPLFLTFCFLNTVAI YTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAE
Sbjct: 367 CLFCGPLFLTFCFLNTVAITYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAE 426

Query: 427 FQAPVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSI 486
           FQAP RTTKYPREIPPLPWYR  IPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSI
Sbjct: 427 FQAPCRTTKYPREIPPLPWYRSAIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSI 486

Query: 487 LFIVFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDM 546
           LFIVFIIL+IVTAFITVALTYFQLAAEDH+WWWRSFLCGGSTGLFIYAYCLYYYYARSDM
Sbjct: 487 LFIVFIILIIVTAFITVALTYFQLAAEDHQWWWRSFLCGGSTGLFIYAYCLYYYYARSDM 546

Query: 547 SGFMQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           SGFMQTSFFFGYMAC+CYGFFLMLG +GFRAALLFVRHIYRSIKCE
Sbjct: 547 SGFMQTSFFFGYMACICYGFFLMLGTVGFRAALLFVRHIYRSIKCE 592

BLAST of Spg036527 vs. TAIR 10
Match: AT5G37310.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 989.2 bits (2556), Expect = 1.4e-288
Identity = 492/583 (84.39%), Postives = 529/583 (90.74%), Query Frame = 0

Query: 10  IALLVLLCGSVQVRSDGSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCVPDDVKE 69
           + + + L G   V SDGSDHRYK GD VPLYANKVGPFHNPSETYRYFDLPFC    VKE
Sbjct: 11  LLVFLFLYGVSPVISDGSDHRYKVGDDVPLYANKVGPFHNPSETYRYFDLPFCSSAPVKE 70

Query: 70  KKEALGEVLNGDRLVSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDKDYYFQMYYD 129
           KKEALGEVLNGDRLVSAPYKL+FL EK+S   C+K+LS+EDVA+FR  + KDYYFQMYYD
Sbjct: 71  KKEALGEVLNGDRLVSAPYKLEFLGEKNSEVACRKRLSREDVAKFRDVIAKDYYFQMYYD 130

Query: 130 DLPIWGFIGKVDKEGK-EPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNALVDLTEDK 189
           DLPIWGF+GKV KEGK +PS++KYYL+ H+ F+IFYNKDRVIEI VRTD N LVDLTEDK
Sbjct: 131 DLPIWGFLGKVVKEGKTDPSEYKYYLFNHLQFEIFYNKDRVIEIIVRTDQNFLVDLTEDK 190

Query: 190 EVDVEFLYTVKWKETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLLLTGFLATI 249
           EV V+F YTV+WKET  PFE RM+KYS +SS+PHHLEIHWFSIINSCVTVLLLTGFLATI
Sbjct: 191 EVQVDFTYTVRWKETEIPFEKRMEKYSLASSMPHHLEIHWFSIINSCVTVLLLTGFLATI 250

Query: 250 LMRVLKNDFVKYAHDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGTQLFTLTVF 309
           LMRVLKNDFVKYAHDEE+ +DQEETGWK IHGDVFR+PKHKSL AA LGSGTQLFTL VF
Sbjct: 251 LMRVLKNDFVKYAHDEEAVDDQEETGWKLIHGDVFRFPKHKSLLAAALGSGTQLFTLAVF 310

Query: 310 IFILALVGVFYPYNRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRNLLLTGCLF 369
           IF+LALVGVFYPYNRGALFTALVVIYALTSGIAGYTA SFYCQLEGTNWVRN++LTG LF
Sbjct: 311 IFMLALVGVFYPYNRGALFTALVVIYALTSGIAGYTAASFYCQLEGTNWVRNVILTGSLF 370

Query: 370 CGPLFLTFCFLNTVAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKNSKAEFQA 429
           CGPL +TF FLNTVAIAY ATAALPFGTIVVI LIW LVTSPLL+LGGIAGKN K+EFQA
Sbjct: 371 CGPLLITFSFLNTVAIAYQATAALPFGTIVVIFLIWALVTSPLLILGGIAGKNRKSEFQA 430

Query: 430 PVRTTKYPREIPPLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILFI 489
           P RTTKYPREIPP+ WYR T+PQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSIL I
Sbjct: 431 PCRTTKYPREIPPMRWYRRTLPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILSI 490

Query: 490 VFIILLIVTAFITVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDMSGF 549
           VF+IL+IVTAFITVALTYFQLAAEDHEWWWRS LCGGSTGLFIYAYCLYYYYARSDMSGF
Sbjct: 491 VFLILVIVTAFITVALTYFQLAAEDHEWWWRSLLCGGSTGLFIYAYCLYYYYARSDMSGF 550

Query: 550 MQTSFFFGYMACVCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           MQTSFFFGYMAC+CYGFFLMLG IGF A+LLFVRHIYRSIKCE
Sbjct: 551 MQTSFFFGYMACICYGFFLMLGTIGFCASLLFVRHIYRSIKCE 593

BLAST of Spg036527 vs. TAIR 10
Match: AT1G08350.2 (Endomembrane protein 70 protein family )

HSP 1 Score: 584.7 bits (1506), Expect = 8.2e-167
Identity = 293/569 (51.49%), Postives = 402/569 (70.65%), Query Frame = 0

Query: 26  GSDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCVPDDVKEKKEALGEVLNGDRLVS 85
           GS + Y  GD VPL+ NKVGP HNPSETY+Y+DLPFC    V EK+E LGEVLNGDRL+S
Sbjct: 24  GSSNHYNAGDHVPLFVNKVGPLHNPSETYQYYDLPFCRRGPVIEKQETLGEVLNGDRLMS 83

Query: 86  APYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDKDYYFQMYYDDLPIWGFIGKVDKE-- 145
           + YKL F ++K    +C+K+L+  D+ARFR  + +DYYFQMYYDDLP+WGF+GKV+ +  
Sbjct: 84  SLYKLKFREDKTHFVLCRKRLTSSDIARFRDIIAQDYYFQMYYDDLPLWGFVGKVEGDYF 143

Query: 146 GKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNALVDLTEDKEVDVEFLYTVKWKET 205
           G+     KYY++ H+ F++ YN D+VIEIN  +DP+ +VD++E+ E+DV+F Y+V W  T
Sbjct: 144 GQGEKHTKYYIFSHLKFNVLYNADKVIEINSFSDPSYMVDISENTEIDVQFTYSVSWNLT 203

Query: 206 TTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLLLTGFLATILMRVLKNDFVKYAHD 265
           +   E RM+KYS++S  P   +IH+FS +NS   V+LL G ++ + MR LKN+   Y+  
Sbjct: 204 SERSETRMNKYSRASFHPISQKIHFFSFLNSITVVVLLIGLISFLFMRHLKNELRSYSIG 263

Query: 266 EESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGTQLFTLTVFIFILALVGVFYPYNR 325
           +E  E+++E GWK +H DVFR P++ S   A LG+GTQL  L + +F LA  G  YPYNR
Sbjct: 264 DE--EERKEAGWKLVHSDVFRCPRNISWLCAILGTGTQLLILIIALFALAFTGFLYPYNR 323

Query: 326 GALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRNLLLTGCLFCGPLFLTFCFLNTVA 385
           G L T+LV++Y LTS +AGYT+TSF+ Q EG    R++ L G L+  P F+    LNTVA
Sbjct: 324 GMLLTSLVIMYTLTSIVAGYTSTSFHSQFEGNKQKRSVRLAGILYPVPFFIILSVLNTVA 383

Query: 386 IAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKN-SKAEFQAPVRTTKYPREIPPL 445
           I Y ATAALPFGTIV+I+LI+TL+  P L+LGG+ G      EFQ P    + PREIPP 
Sbjct: 384 ITYGATAALPFGTIVIIILIFTLLNIPFLMLGGVLGNRFGLLEFQPPSAVKRNPREIPPQ 443

Query: 446 PWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILLIVTAFITV 505
            WYR  + Q+ + GF+PFSA+ +E + ++AS+WG +IYT   I+   FI+L+ +++ + +
Sbjct: 444 NWYRRKLYQVFLGGFVPFSAVVLEWHQLYASLWGFKIYTSPGIMLFTFIVLIFLSSSVGI 503

Query: 506 ALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDMSGFMQTSFFFGYMACVC 565
            LTY QL+ EDHEWWWRS LCGG T +F+Y Y +  +Y RSDM+GF+Q SF+ GY A +C
Sbjct: 504 ILTYIQLSGEDHEWWWRSILCGGFTAVFMYGYGV-LFYLRSDMTGFLQLSFYLGYTALLC 563

Query: 566 YGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           Y  FL+LG I F A+L+F+RHIYRS+K E
Sbjct: 564 YALFLVLGTISFLASLMFIRHIYRSVKLE 589

BLAST of Spg036527 vs. TAIR 10
Match: AT1G08350.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 498.4 bits (1282), Expect = 7.7e-141
Identity = 253/511 (49.51%), Postives = 357/511 (69.86%), Query Frame = 0

Query: 84  VSAPYKLDFLQEKDSLSVCKKKLSKEDVARFRAAVDKDYYFQMYYDDLPIWGFIGKVDKE 143
           +S+ YKL F ++K    +C+K+L+  D+ARFR  + +DYYFQMYYDDLP+WGF+GKV+ +
Sbjct: 1   MSSLYKLKFREDKTHFVLCRKRLTSSDIARFRDIIAQDYYFQMYYDDLPLWGFVGKVEGD 60

Query: 144 --GKEPSDFKYYLYKHIHFDIFYNKDRVIEINVRTDPNALVDLTEDKEVDVEFLYTVKWK 203
             G+     KYY++ H+ F++ YN D+VIEIN  +DP+ +VD++E+ E+DV+F Y+V W 
Sbjct: 61  YFGQGEKHTKYYIFSHLKFNVLYNADKVIEINSFSDPSYMVDISENTEIDVQFTYSVSWN 120

Query: 204 ETTTPFENRMDKYSQSSSLPHHLEIHWFSIINSCVTVLLLTGFLATILMRVLKNDFVKYA 263
            T+   E RM+KYS++S  P   +IH+FS +NS   V+LL G ++ + MR LKN+   Y+
Sbjct: 121 LTSERSETRMNKYSRASFHPISQKIHFFSFLNSITVVVLLIGLISFLFMRHLKNELRSYS 180

Query: 264 HDEESAEDQEETGWKYIHGDVFRYPKHKSLFAACLGSGTQLFTLTVFIFILALVGVFYPY 323
             +E  E+++E GWK +H DVFR P++ S   A LG+GTQL  L + +F LA  G  YPY
Sbjct: 181 IGDE--EERKEAGWKLVHSDVFRCPRNISWLCAILGTGTQLLILIIALFALAFTGFLYPY 240

Query: 324 NRGALFTALVVIYALTSGIAGYTATSFYCQLEGTNWVRNLLLTGCLFCGPLFLTFCFLNT 383
           NRG L T+LV++Y LTS +AGYT+TSF+ Q EG    R++ L G L+  P F+    LNT
Sbjct: 241 NRGMLLTSLVIMYTLTSIVAGYTSTSFHSQFEGNKQKRSVRLAGILYPVPFFIILSVLNT 300

Query: 384 VAIAYTATAALPFGTIVVIVLIWTLVTSPLLVLGGIAGKN-SKAEFQAPVRTTKYPREIP 443
           VAI Y ATAALPFGTIV+I+LI+TL+  P L+LGG+ G      EFQ P    + PREIP
Sbjct: 301 VAITYGATAALPFGTIVIIILIFTLLNIPFLMLGGVLGNRFGLLEFQPPSAVKRNPREIP 360

Query: 444 PLPWYRGTIPQMAMAGFLPFSAIYIELYYIFASVWGHRIYTIYSILFIVFIILLIVTAFI 503
           P  WYR  + Q+ + GF+PFSA+ +E + ++AS+WG +IYT   I+   FI+L+ +++ +
Sbjct: 361 PQNWYRRKLYQVFLGGFVPFSAVVLEWHQLYASLWGFKIYTSPGIMLFTFIVLIFLSSSV 420

Query: 504 TVALTYFQLAAEDHEWWWRSFLCGGSTGLFIYAYCLYYYYARSDMSGFMQTSFFFGYMAC 563
            + LTY QL+ EDHEWWWRS LCGG T +F+Y Y +  +Y RSDM+GF+Q SF+ GY A 
Sbjct: 421 GIILTYIQLSGEDHEWWWRSILCGGFTAVFMYGYGV-LFYLRSDMTGFLQLSFYLGYTAL 480

Query: 564 VCYGFFLMLGAIGFRAALLFVRHIYRSIKCE 592
           +CY  FL+LG I F A+L+F+RHIYRS+K E
Sbjct: 481 LCYALFLVLGTISFLASLMFIRHIYRSVKLE 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894421.10.0e+0098.65transmembrane 9 superfamily member 3 [Benincasa hispida][more]
XP_008461818.10.0e+0098.82PREDICTED: transmembrane 9 superfamily member 3 isoform X1 [Cucumis melo] >KAA00... [more]
XP_022924223.10.0e+0098.31transmembrane 9 superfamily member 3-like [Cucurbita moschata] >XP_023519963.1 t... [more]
XP_022137162.10.0e+0098.14transmembrane 9 superfamily member 3 [Momordica charantia][more]
XP_023524630.10.0e+0098.14transmembrane 9 superfamily member 3 [Cucurbita pepo subsp. pepo] >KAG7037065.1 ... [more]
Match NameE-valueIdentityDescription
Q9ZPS71.8e-30488.57Transmembrane 9 superfamily member 3 OS=Arabidopsis thaliana OX=3702 GN=TMN3 PE=... [more]
Q940S02.2e-30287.37Transmembrane 9 superfamily member 2 OS=Arabidopsis thaliana OX=3702 GN=TMN2 PE=... [more]
Q9FHT42.0e-28784.39Transmembrane 9 superfamily member 4 OS=Arabidopsis thaliana OX=3702 GN=TMN4 PE=... [more]
F4HW171.2e-16551.49Transmembrane 9 superfamily member 5 OS=Arabidopsis thaliana OX=3702 GN=TMN5 PE=... [more]
Q54ZW03.4e-15746.51Putative phagocytic receptor 1b OS=Dictyostelium discoideum OX=44689 GN=phg1b PE... [more]
Match NameE-valueIdentityDescription
A0A5A7SZM30.0e+0098.82Transmembrane 9 superfamily member OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1EBS70.0e+0098.31Transmembrane 9 superfamily member OS=Cucurbita moschata OX=3662 GN=LOC111431735... [more]
A0A1S3CG210.0e+0098.82Transmembrane 9 superfamily member OS=Cucumis melo OX=3656 GN=LOC103500066 PE=3 ... [more]
A0A6J1C7H60.0e+0098.14Transmembrane 9 superfamily member OS=Momordica charantia OX=3673 GN=LOC11100870... [more]
A0A6J1KLE40.0e+0097.80Transmembrane 9 superfamily member OS=Cucurbita maxima OX=3661 GN=LOC111495631 P... [more]
Match NameE-valueIdentityDescription
AT2G01970.11.3e-30588.57Endomembrane protein 70 protein family [more]
AT1G14670.11.6e-30387.37Endomembrane protein 70 protein family [more]
AT5G37310.11.4e-28884.39Endomembrane protein 70 protein family [more]
AT1G08350.28.2e-16751.49Endomembrane protein 70 protein family [more]
AT1G08350.17.7e-14149.51Endomembrane protein 70 protein family [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004240Nonaspanin (TM9SF)PFAMPF02990EMP70coord: 54..548
e-value: 1.1E-170
score: 568.7
IPR004240Nonaspanin (TM9SF)PANTHERPTHR10766TRANSMEMBRANE 9 SUPERFAMILY PROTEINcoord: 9..591
NoneNo IPR availablePANTHERPTHR10766:SF136TRANSMEMBRANE 9 SUPERFAMILY MEMBERcoord: 9..591
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 224..408

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg036527.1Spg036527.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0072657 protein localization to membrane
cellular_component GO:0010008 endosome membrane
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005802 trans-Golgi network