Spg036511 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg036511
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein kinase domain-containing protein
Locationscaffold5: 44808159 .. 44811822 (+)
RNA-Seq ExpressionSpg036511
SyntenySpg036511
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAGAAGAATATTTCCTCATCTTCTTCTTCCCCAACCCCACAGATCTCTGGTTCTTCAATCCTCCTCTTCCTTTCGAGACTCTGCTTCTTTCTCAGAGATCATGGAGATGGAGCGCATGATCGAGTTTCCTCACACGCATCTTGATCGGAGGCCCCGAAAGAGGGCTCGTTTGGGCTGGGACGTTGTCCCTGAGGCCCCTAAGGTATAATCTCCCATCCTCTCTTTCAGATCTGATTTTATTTTGTGTTGGTTTTGGTTTTGTAGTGCTAGATCTGCCGGATTTTTCTTTGTTTAGGTATGTATTAGTGTCACACACCGAGGTATCGTCTCTGATCAACGTAGCTTAAGAAGCCTTTCGTTTTGTGTTCTTTGTTAGCGTAGATTTACCTTTTCTGATGGTTGATTCACGTTTTTTTCCCTCGAGATTCTTGATTATTCTATGAGTATAGACTATTTTCGTGAGGTTTCTTTATTAATCTGTTGAGTTAACGAATTTTTATGTTATCTGTAAGCTCGAATGAATTATCTTGTCAGATCAGGTAAGAATCGTTCTTTAATTAGATGGATGATTATTCGAATCTTTTATTTCTTTTGTTATTGATTATTTTTCCTTATAATTTTTTTGTTTCTTTCCACGATTTGTTTAAACTAGGCCTTTCCCTTTCCTTGCCCTTCGTTCTTGTCTGTTTACCATGCATCTGGCTTATGTTTCATATTTGTCCGATTATTTGCCTCCCATCTGTATTCATTCAACAAAATCTTGCAATTTCTTTTGTGTTATGGAAAATCAAGACTTTGGGTGAGGATGTTAGGTTTAGGAAGGGCAAACTTTTATATGAATGGGTGACTGTGTGTAGGCTCAGGTAGGAATATGTTGTGGACAAGAGATTGCGAATATGCCAAGCTTTGCATCTTCAAGAGCACCTTCAGATCATTCTTCTAATCCACTATTTGTTAAGGGAGTGGCTCGAAATGGTTCTCCCCCCTGGCGAGAAGATGACAAAGATGGGCATTACATGTTTGCGCTTGGAGAAAATTTAACTTCTCGCTGTAAATACACTTGACTATGATTAAAGAAATTTTTATGTGCTCAGAATTGATCATCGTAGATTTTGTTCTCTTTGTAAATTAGTTCCTCTGCTTATTTAAATGATGATGGCTATAATCTTCCAACTGAAGACAGACTGTATTTAAAACAGTATCCATCATTGTCACCTACATTTATTAATTTTATTTCTCAAAATTGAACTCACCTCCACTCGTTATGTTTTTCAAAATCATTACTTCTTGGAATTGTTTAGGACTAGTAAGGTTTTGGTAATTATAATCTCTTTTCTGATGGTTGTTTCTTATTCTTACTCTGTCAATTTTTTCTTCTGTGGCAGATAAAATACATAGCAAAATGGGTGAAGGTACCTTTGGGCAGGTTTTAGAATGCTGGGACAGAGAAAGAAAGGAAATGGTTGCAATAAAAATTGTGCGTGGAATCAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTGCAACAGCTTGGAAAGCACGATAAAGGTGGCAACCGGTGAGTATCCTTTTTGTTCTAAATCTTCCTTACTGATGTTGTTGGCACTCTGTGGCTTTTGTCATCTTTGTGGCTATGGTTAAATGGCTTTATTCCTTGCAGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATCTGTATTGTAAGTATAAGGCTGTTTTATCATGGATTAATTTCCCCGATTCACCAAGTATGGGGGGTTAAGATTTTTGGGTGAACTTAAATGGTTTTAACAGGTGTTTGAGAAGCTTGGACCAAGCTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAATGTGTAGCATGTGTGTAGTATTTTATTGAACCTAGTCCTTTCTTTTCTTTCCCATTCTTAGAGTCGTATGTTTGTTGCAAAACACTCCACAAATTTCTTCAAGTTAAATTGTGATTAGCATCTGCTGCATGTTTCATTTTGTTTTCTGCAATTTTATTTAAATTATCTGACGAGTCAGTTTCTTAGTATTTTGTGTTCTGTATTCTAATAGTTTCTTTGACCCTTGAAATCCAGTCATGCATGACCTACGAATGATACATACCGACTTGAAGCCAGAGAACATACTATTGGTCTCTGCAGATTATGTGAAAGTACATGACTATAAGGTACTTGTTTTGTGTCTAGTTTTTTATTGGAATTTCCTACTATTTAGTTCTCTTTGGTCTGGTTTCTCTAATTATTTTGACCTTTTGTGGCAGAATTTGTCTCGATCGCCAAGGGATAGTTCTAACTTCAAAAGAGTGCCCAAGTCAAGTGCTATAAAGGTGATTGATTTTGGTAGCACCACTTATGATCGTCAAGATCAGAATTACATTGTATCTACACGCCATTATCGAGCTCCAGAGGTTATTCTTGGTAAGGAATTTTGATTAAATTTCATATACATATATATATATATCTTTTATCTTTTGAGTATTTTGATTTGTGGTGAGTTCATGACTTGACCATATTAATTATTAATTGTGTAGGTCTAGGATGGAGTTTTCCTTGTGATATATGGAGTGTTGGCTGTATCCTTGTTGAACTGTGCTCGGTATGTGTTTCATTTGTCTGTAATACGTCTGTTATCTAGATTTTTTGTCTATTTATCCAATATCTTGTTGAATGAGGTAGTTATTGAGTTTTCAATGCAATATCATGTTTAGGGTGAAGCACTGTTTCAAACGCATGAGAACTTAGAGCATTTAGCAATGATGGAGCGTGTGCTCGGTCCATTGCCACAGGAGATGCTGAAGAGAGTAGAGTAAGTATTTCTAATCCTTTATATTCTTGGGTTTTTTTTTTTTTTGATATTATATTCTTGTTGTTAAGCAGACGATAAATAACTGAATGCTGAAGTTCGTTCTTTTTTCTTTGTATATATCAATAGTCGGCATGCGGAAAAATATGTTAGAAGAGGTAGATTAGATTGGCCAGAAGGTGCAACTTCAAGGGATAGTATGAAGGCCGTTCTAAAGCTTCCTCGTTTACCGGTTTGTATTTTAATAATATGTTTTACCGGTTTGATGGGACTTTTTTGTTTTGTAACTTTACACTAATTATCTCGTGAAAATTCATGCAGAACTTGATAATGCAGCATGTGGACCATTCAGCCGGTGAATTGATTCATCTTGTGCAAGGGTTGCTTCGATACGATCCCGTGCATAGATTAACGGCTCGTGAAGCCCTAAGGCATCCATTCTTTACAAGGGATCCCTTAAGGAGATAGTAGCTGTTGTTGGCTTATTAACTGACTAGCATTTGTGTTTTTTACACTCTTCGCCCATCGTCTTCATGCAACTGTATAGCTGTGCGAGTCTGTGATCGATGATCGTGATGCCCTCTGTGGTCCTCGGCCAGAAGAGAGGAGTATCTGGCTTTGATGATGTAAATATAAGTTTACTCCAAGCAGATTTTTGCAAAGCTAGCACTAGTTTGCTCAATTTGCACTCCATTCCTAATGTTTAAAAATTGTTCAGAGAAATATACGCTAACTTAATTTTTACACTATTTTTGATGTACCTGCAAAATGTTTGACAATTACTTG

mRNA sequence

ATGGAGATGGAGCGCATGATCGAGTTTCCTCACACGCATCTTGATCGGAGGCCCCGAAAGAGGGCTCGTTTGGGCTGGGACGTTGTCCCTGAGGCCCCTAAGGCTCAGGTAGGAATATGTTGTGGACAAGAGATTGCGAATATGCCAAGCTTTGCATCTTCAAGAGCACCTTCAGATCATTCTTCTAATCCACTATTTGTTAAGGGAGTGGCTCGAAATGGTTCTCCCCCCTGGCGAGAAGATGACAAAGATGGGCATTACATGTTTGCGCTTGGAGAAAATTTAACTTCTCGCTATAAAATACATAGCAAAATGGGTGAAGGTACCTTTGGGCAGGTTTTAGAATGCTGGGACAGAGAAAGAAAGGAAATGGTTGCAATAAAAATTGTGCGTGGAATCAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTGCAACAGCTTGGAAAGCACGATAAAGGTGGCAACCGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATCTGTATTGTGTTTGAGAAGCTTGGACCAAGCTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAATGTGTAGCATTCATGCATGACCTACGAATGATACATACCGACTTGAAGCCAGAGAACATACTATTGGTCTCTGCAGATTATGTGAAAGTACATGACTATAAGAATTTGTCTCGATCGCCAAGGGATAGTTCTAACTTCAAAAGAGTGCCCAAGTCAAGTGCTATAAAGGTGATTGATTTTGGTAGCACCACTTATGATCGTCAAGATCAGAATTACATTGTATCTACACGCCATTATCGAGCTCCAGAGGTTATTCTTGGTCTAGGATGGAGTTTTCCTTGTGATATATGGAGTGTTGGCTGTATCCTTGTTGAACTGTGCTCGGGTGAAGCACTGTTTCAAACGCATGAGAACTTAGAGCATTTAGCAATGATGGAGCGTGTGCTCGGTCCATTGCCACAGGAGATGCTGAAGAGAGTAGATCGGCATGCGGAAAAATATGTTAGAAGAGGTAGATTAGATTGGCCAGAAGGTGCAACTTCAAGGGATAGTATGAAGGCCGTTCTAAAGCTTCCTCGTTTACCGAACTTGATAATGCAGCATGTGGACCATTCAGCCGGTGAATTGATTCATCTTGTGCAAGGGTTGCTTCGATACGATCCCGTGCATAGATTAACGGCTCGTGAAGCCCTAAGGCATCCATTCTTTACAAGGGATCCCTTAAGGAGATAG

Coding sequence (CDS)

ATGGAGATGGAGCGCATGATCGAGTTTCCTCACACGCATCTTGATCGGAGGCCCCGAAAGAGGGCTCGTTTGGGCTGGGACGTTGTCCCTGAGGCCCCTAAGGCTCAGGTAGGAATATGTTGTGGACAAGAGATTGCGAATATGCCAAGCTTTGCATCTTCAAGAGCACCTTCAGATCATTCTTCTAATCCACTATTTGTTAAGGGAGTGGCTCGAAATGGTTCTCCCCCCTGGCGAGAAGATGACAAAGATGGGCATTACATGTTTGCGCTTGGAGAAAATTTAACTTCTCGCTATAAAATACATAGCAAAATGGGTGAAGGTACCTTTGGGCAGGTTTTAGAATGCTGGGACAGAGAAAGAAAGGAAATGGTTGCAATAAAAATTGTGCGTGGAATCAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTGCAACAGCTTGGAAAGCACGATAAAGGTGGCAACCGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATCTGTATTGTGTTTGAGAAGCTTGGACCAAGCTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAATGTGTAGCATTCATGCATGACCTACGAATGATACATACCGACTTGAAGCCAGAGAACATACTATTGGTCTCTGCAGATTATGTGAAAGTACATGACTATAAGAATTTGTCTCGATCGCCAAGGGATAGTTCTAACTTCAAAAGAGTGCCCAAGTCAAGTGCTATAAAGGTGATTGATTTTGGTAGCACCACTTATGATCGTCAAGATCAGAATTACATTGTATCTACACGCCATTATCGAGCTCCAGAGGTTATTCTTGGTCTAGGATGGAGTTTTCCTTGTGATATATGGAGTGTTGGCTGTATCCTTGTTGAACTGTGCTCGGGTGAAGCACTGTTTCAAACGCATGAGAACTTAGAGCATTTAGCAATGATGGAGCGTGTGCTCGGTCCATTGCCACAGGAGATGCTGAAGAGAGTAGATCGGCATGCGGAAAAATATGTTAGAAGAGGTAGATTAGATTGGCCAGAAGGTGCAACTTCAAGGGATAGTATGAAGGCCGTTCTAAAGCTTCCTCGTTTACCGAACTTGATAATGCAGCATGTGGACCATTCAGCCGGTGAATTGATTCATCTTGTGCAAGGGTTGCTTCGATACGATCCCGTGCATAGATTAACGGCTCGTGAAGCCCTAAGGCATCCATTCTTTACAAGGGATCCCTTAAGGAGATAG

Protein sequence

MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREALRHPFFTRDPLRR
Homology
BLAST of Spg036511 vs. NCBI nr
Match: XP_038894261.1 (serine/threonine-protein kinase AFC2 isoform X1 [Benincasa hispida])

HSP 1 Score: 885.9 bits (2288), Expect = 1.4e-253
Identity = 423/433 (97.69%), Postives = 430/433 (99.31%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIAN+PSFAS+RAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANIPSFASTRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           +KE VAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 KKETVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVS+DYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300
           HDYKNLSRSPRDSSNFKRVP+SSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPRSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREA 420
           GRLDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAGELIHLVQGLLRYDP+HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGELIHLVQGLLRYDPMHRLTAREA 420

Query: 421 LRHPFFTRDPLRR 434
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of Spg036511 vs. NCBI nr
Match: XP_023519374.1 (serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 885.6 bits (2287), Expect = 1.8e-253
Identity = 424/433 (97.92%), Postives = 428/433 (98.85%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADY+KV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300
           HDYKN SRSPRDSSNFKRVPKSS+IKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSSIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREA 420
           GRLDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAGELIHLVQGLLRYDP HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 420

Query: 421 LRHPFFTRDPLRR 434
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of Spg036511 vs. NCBI nr
Match: XP_023001260.1 (serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 884.4 bits (2284), Expect = 3.9e-253
Identity = 424/433 (97.92%), Postives = 427/433 (98.61%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADY+KV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300
           HDYKN SRSPRDSSNFKRVPKSS IKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREA 420
           GRLDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAGELIHLVQGLLRYDP HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 420

Query: 421 LRHPFFTRDPLRR 434
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of Spg036511 vs. NCBI nr
Match: XP_022923920.1 (serine/threonine-protein kinase AFC2 isoform X1 [Cucurbita moschata] >KAG6584116.1 Serine/threonine-protein kinase AFC2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019705.1 Serine/threonine-protein kinase AFC2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 884.4 bits (2284), Expect = 3.9e-253
Identity = 423/433 (97.69%), Postives = 428/433 (98.85%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADY+KV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300
           HDYKN SRSPRDSSNFKRVPKSS+IKVIDFGSTTYDR+DQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSSIKVIDFGSTTYDREDQNYIVSTRHYRAPEVILGIGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREA 420
           GRLDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAGELIHLVQGLLRYDP HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 420

Query: 421 LRHPFFTRDPLRR 434
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of Spg036511 vs. NCBI nr
Match: XP_008439081.1 (PREDICTED: serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo] >KAA0055279.1 serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo var. makuwa] >TYJ99202.1 serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 882.9 bits (2280), Expect = 1.1e-252
Identity = 421/433 (97.23%), Postives = 428/433 (98.85%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIAN+PSFAS+RAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANIPSFASTRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           +KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 KKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDI RQLLECVAFMHDLRMIHTDLKPENILLVS DYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIARQLLECVAFMHDLRMIHTDLKPENILLVSGDYVKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300
           HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQ+MLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQDMLKKVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREA 420
           GRLDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAGELIHLVQGLLRYDP HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGELIHLVQGLLRYDPAHRLTAREA 420

Query: 421 LRHPFFTRDPLRR 434
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of Spg036511 vs. ExPASy Swiss-Prot
Match: P51567 (Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=1 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 2.1e-220
Identity = 366/428 (85.51%), Postives = 402/428 (93.93%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMER+ EFPHTH+DRRPRKRARLGWDV+P+A KAQVG+ CGQEI N+ SFASS APSD+
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQVGMFCGQEIGNISSFASSGAPSDN 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SS+ L VKGVARNGSPPWREDDKDGHY+F LG++LT RYKI+SKMGEGTFGQVLECWDRE
Sbjct: 61  SSS-LCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVA+KIVRG++KYR+AAMIEIE+LQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKV 240
           G SLYDFLRKNNYRSFPIDLVR+IG QLLECVAFMHDLRMIHTDLKPENILLVS+DYVK+
Sbjct: 181 GSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKI 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300
            +YK  SR  RD   +KRVPKSSAIKVIDFGSTTY+RQDQ YIVSTRHYRAPEVILGLGW
Sbjct: 241 PEYKG-SRLQRDVC-YKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360
           S+PCD+WSVGCI+VELC+GEALFQTHENLEHLAMMERVLGP PQ+MLK+VDRH+EKYVRR
Sbjct: 301 SYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREA 420
           GRLDWP+GATSRDS+KAVLKLPRL NLIMQHVDHSAGELI++VQGLLR+DP  R+TAREA
Sbjct: 361 GRLDWPDGATSRDSLKAVLKLPRLQNLIMQHVDHSAGELINMVQGLLRFDPSERITAREA 420

Query: 421 LRHPFFTR 429
           LRHPFF R
Sbjct: 421 LRHPFFAR 425

BLAST of Spg036511 vs. ExPASy Swiss-Prot
Match: P51566 (Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=2 SV=2)

HSP 1 Score: 647.1 bits (1668), Expect = 1.4e-184
Identity = 309/430 (71.86%), Postives = 357/430 (83.02%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPE-APKAQVGICCGQEIANMPSFASSRAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P   P           +   P FAS   P+ 
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 76

Query: 61  HSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDR 120
              N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQVLEC+D 
Sbjct: 77  VYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 136

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEK 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNHICIVFEK
Sbjct: 137 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 196

Query: 181 LGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVK 240
           LGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR+IHTDLKPENILLVS++Y+K
Sbjct: 197 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 256

Query: 241 VHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLG 300
           + DYK LSR  +D S FK +PKSSAIK+IDFGSTT++ QD NYIVSTRHYRAPEVILG+G
Sbjct: 257 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 316

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVR 360
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR +EKY R
Sbjct: 317 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 376

Query: 361 RG-RLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAR 420
           RG +LDWPEGATSRDS+KAV KLPRLPNLIMQHVDHSAG+LI L+QGLLRYDP  R  AR
Sbjct: 377 RGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAR 436

Query: 421 EALRHPFFTR 429
           EAL HPFFTR
Sbjct: 437 EALNHPFFTR 445

BLAST of Spg036511 vs. ExPASy Swiss-Prot
Match: P51568 (Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=1 SV=2)

HSP 1 Score: 510.4 bits (1313), Expect = 2.0e-143
Identity = 243/388 (62.63%), Postives = 305/388 (78.61%), Query Frame = 0

Query: 43  QEIANMPSFASSRAPSDHSSNPLFVKGVARNG---SPPWREDDKDGHYMFALGENLTSRY 102
           + +   P      AP++  +    +KG   +G   SPP R+DD+DGHY+F+L +NLT RY
Sbjct: 12  ERVRKRPRMTWDEAPAEPEAKRAVIKGHGSDGRILSPPLRDDDRDGHYVFSLRDNLTPRY 71

Query: 103 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 162
           KI SKMGEGTFG+VLECWDR+ KE VAIKI+R I+KYRDAAMIEI+VLQ+L K DKG  R
Sbjct: 72  KILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKSDKGRTR 131

Query: 163 CVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLR 222
           CVQ++NWFDYRNHICIVFEKLGPSL+DFL++N Y +FP+ LVRD G QLLE VA+MH+L+
Sbjct: 132 CVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAFPLALVRDFGCQLLESVAYMHELQ 191

Query: 223 MIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQD 282
           ++HTDLKPENILLVS++ VK+ D K   RS  + ++F+ +PKSSAIK+IDFGST  D + 
Sbjct: 192 LVHTDLKPENILLVSSENVKLPDNK---RSAANETHFRCLPKSSAIKLIDFGSTVCDNRI 251

Query: 283 QNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVL 342
            + IV TRHYR+PEVILGLGWS+ CD+WS+GCIL ELC+GEALFQTH+NLEHLAMMER L
Sbjct: 252 HHSIVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAMMERAL 311

Query: 343 GPLPQEMLKRVDRHAEKYVRRG-RLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGE 402
           GPLP+ M ++  R AEKY RRG RL+WPEGA SR+S++AV +L RL +++ +HVD++   
Sbjct: 312 GPLPEHMTRKASRGAEKYFRRGCRLNWPEGANSRESIRAVKRLDRLKDMVSKHVDNTRSR 371

Query: 403 LIHLVQGLLRYDPVHRLTAREALRHPFF 427
              L+ GLL YDP  RLTA EAL HPFF
Sbjct: 372 FADLLYGLLAYDPSERLTANEALDHPFF 396

BLAST of Spg036511 vs. ExPASy Swiss-Prot
Match: P49760 (Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.1e-91
Identity = 178/377 (47.21%), Postives = 246/377 (65.25%), Query Frame = 0

Query: 54  SRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQV 113
           SR+ S HSS     K V         EDD +GH ++ +G+ L  RY+I S +GEGTFG+V
Sbjct: 129 SRSSSQHSSRR--AKSV---------EDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRV 188

Query: 114 LECWDRER-KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGG-NRCVQIRNWFDYRN 173
           ++C D  R    VA+KI++ + KY++AA +EI VL+++ + D    N CVQ+ +WFDY  
Sbjct: 189 VQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHG 248

Query: 174 HICIVFEKLGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENIL 233
           H+CI FE LG S +DFL+ NNY  +PI  VR +  QL + V F+HD ++ HTDLKPENIL
Sbjct: 249 HMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENIL 308

Query: 234 LVSADYVKVHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRA 293
            V++DY   +   NL +  RD    +R  KS+A++V+DFGS T+D +  + IVSTRHYRA
Sbjct: 309 FVNSDYELTY---NLEKK-RD----ERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRA 368

Query: 294 PEVILGLGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVD 353
           PEVIL LGWS PCD+WS+GCI+ E   G  LFQTH+N EHLAMMER+LGP+P  M+++  
Sbjct: 369 PEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPIPSRMIRKTR 428

Query: 354 RHAEKYVRRGRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDP 413
           +  +KY  RGRLDW E  ++   ++   K P    L  +  +H   +L  L++ +L Y+P
Sbjct: 429 K--QKYFYRGRLDWDENTSAGRYVRENCK-PLRRYLTSEAEEHH--QLFDLIESMLEYEP 481

Query: 414 VHRLTAREALRHPFFTR 429
             RLT  EAL+HPFF R
Sbjct: 489 AKRLTLGEALQHPFFAR 481

BLAST of Spg036511 vs. ExPASy Swiss-Prot
Match: O35491 (Dual specificity protein kinase CLK2 OS=Mus musculus OX=10090 GN=Clk2 PE=1 SV=2)

HSP 1 Score: 337.8 bits (865), Expect = 1.8e-91
Identity = 170/349 (48.71%), Postives = 236/349 (67.62%), Query Frame = 0

Query: 80  EDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRER-KEMVAIKIVRGIRKYRD 139
           EDD +GH ++ +G+ L  RY+I S +GEGTFG+V++C D  R    VA+KI++ + KY++
Sbjct: 143 EDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGTQVALKIIKNVEKYKE 202

Query: 140 AAMIEIEVLQQLGKHDKGG-NRCVQIRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFP 199
           AA +EI VL+++ + D    N CVQ+ +WFDY  H+CI FE LG S +DFL+ NNY  +P
Sbjct: 203 AARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYP 262

Query: 200 IDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKVHDYKNLSRSPRDSSNFK 259
           I  VR +  QL + V F+HD ++ HTDLKPENIL V++DY   +   NL +  RD    +
Sbjct: 263 IHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTY---NLEKK-RD----E 322

Query: 260 RVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGWSFPCDIWSVGCILVELC 319
           R  KS+A++V+DFGS T+D +  + IVSTRHYRAPEVIL LGWS PCD+WS+GCI+ E  
Sbjct: 323 RSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYY 382

Query: 320 SGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSMKA 379
            G  LFQTH+N EHLAMMER+LGP+P  M+++  +  +KY  RGRLDW E  ++   ++ 
Sbjct: 383 VGFTLFQTHDNREHLAMMERILGPVPSRMIRKTRK--QKYFYRGRLDWDENTSAGRYVRE 442

Query: 380 VLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREALRHPFF 427
             K P    L  +  DH   +L  L++ +L Y+P  RLT  EAL+HPFF
Sbjct: 443 NCK-PLRRYLTSEAEDHH--QLFDLIENMLEYEPAKRLTLGEALQHPFF 478

BLAST of Spg036511 vs. ExPASy TrEMBL
Match: A0A6J1KKP8 (serine/threonine-protein kinase AFC2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495446 PE=4 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 1.9e-253
Identity = 424/433 (97.92%), Postives = 427/433 (98.61%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADY+KV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300
           HDYKN SRSPRDSSNFKRVPKSS IKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREA 420
           GRLDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAGELIHLVQGLLRYDP HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 420

Query: 421 LRHPFFTRDPLRR 434
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of Spg036511 vs. ExPASy TrEMBL
Match: A0A6J1EDB4 (serine/threonine-protein kinase AFC2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431493 PE=4 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 1.9e-253
Identity = 423/433 (97.69%), Postives = 428/433 (98.85%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADY+KV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300
           HDYKN SRSPRDSSNFKRVPKSS+IKVIDFGSTTYDR+DQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSSIKVIDFGSTTYDREDQNYIVSTRHYRAPEVILGIGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREA 420
           GRLDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAGELIHLVQGLLRYDP HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREA 420

Query: 421 LRHPFFTRDPLRR 434
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of Spg036511 vs. ExPASy TrEMBL
Match: A0A5A7UP87 (Serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004120 PE=4 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 5.6e-253
Identity = 421/433 (97.23%), Postives = 428/433 (98.85%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIAN+PSFAS+RAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANIPSFASTRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           +KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 KKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDI RQLLECVAFMHDLRMIHTDLKPENILLVS DYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIARQLLECVAFMHDLRMIHTDLKPENILLVSGDYVKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300
           HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQ+MLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQDMLKKVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREA 420
           GRLDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAGELIHLVQGLLRYDP HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGELIHLVQGLLRYDPAHRLTAREA 420

Query: 421 LRHPFFTRDPLRR 434
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of Spg036511 vs. ExPASy TrEMBL
Match: A0A6J1C5J1 (serine/threonine-protein kinase AFC2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008590 PE=4 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 5.6e-253
Identity = 422/433 (97.46%), Postives = 429/433 (99.08%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY+IHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYRIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVS DYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSVDYVKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300
           HDYKNLSRSPRDS+NFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW
Sbjct: 241 HDYKNLSRSPRDSTNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKKVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREA 420
           GRLDWPEGATSRDS+KAV KLPRLPNLIMQHVDHSAGELI LVQGLLRY+PV RLTAR+A
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLPRLPNLIMQHVDHSAGELIQLVQGLLRYNPVDRLTARQA 420

Query: 421 LRHPFFTRDPLRR 434
           L+HPFFTRDPLRR
Sbjct: 421 LKHPFFTRDPLRR 433

BLAST of Spg036511 vs. ExPASy TrEMBL
Match: A0A1S3AXH4 (serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483972 PE=4 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 5.6e-253
Identity = 421/433 (97.23%), Postives = 428/433 (98.85%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIAN+PSFAS+RAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANIPSFASTRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           +KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 KKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKV 240
           GPSLYDFLRKNNYRSFPIDLVRDI RQLLECVAFMHDLRMIHTDLKPENILLVS DYVKV
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIARQLLECVAFMHDLRMIHTDLKPENILLVSGDYVKV 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300
           HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQ+MLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQDMLKKVDRHAEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREA 420
           GRLDWPEGATSRDS+KAV KL RLPNLIMQHVDHSAGELIHLVQGLLRYDP HRLTAREA
Sbjct: 361 GRLDWPEGATSRDSIKAVQKLARLPNLIMQHVDHSAGELIHLVQGLLRYDPAHRLTAREA 420

Query: 421 LRHPFFTRDPLRR 434
           LRHPFFTRDPLRR
Sbjct: 421 LRHPFFTRDPLRR 433

BLAST of Spg036511 vs. TAIR 10
Match: AT4G24740.1 (FUS3-complementing gene 2 )

HSP 1 Score: 766.1 bits (1977), Expect = 1.5e-221
Identity = 366/428 (85.51%), Postives = 402/428 (93.93%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMER+ EFPHTH+DRRPRKRARLGWDV+P+A KAQVG+ CGQEI N+ SFASS APSD+
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQVGMFCGQEIGNISSFASSGAPSDN 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SS+ L VKGVARNGSPPWREDDKDGHY+F LG++LT RYKI+SKMGEGTFGQVLECWDRE
Sbjct: 61  SSS-LCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180
           RKEMVA+KIVRG++KYR+AAMIEIE+LQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL
Sbjct: 121 RKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVKV 240
           G SLYDFLRKNNYRSFPIDLVR+IG QLLECVAFMHDLRMIHTDLKPENILLVS+DYVK+
Sbjct: 181 GSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKI 240

Query: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300
            +YK  SR  RD   +KRVPKSSAIKVIDFGSTTY+RQDQ YIVSTRHYRAPEVILGLGW
Sbjct: 241 PEYKG-SRLQRDVC-YKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360
           S+PCD+WSVGCI+VELC+GEALFQTHENLEHLAMMERVLGP PQ+MLK+VDRH+EKYVRR
Sbjct: 301 SYPCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRR 360

Query: 361 GRLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAREA 420
           GRLDWP+GATSRDS+KAVLKLPRL NLIMQHVDHSAGELI++VQGLLR+DP  R+TAREA
Sbjct: 361 GRLDWPDGATSRDSLKAVLKLPRLQNLIMQHVDHSAGELINMVQGLLRFDPSERITAREA 420

Query: 421 LRHPFFTR 429
           LRHPFF R
Sbjct: 421 LRHPFFAR 425

BLAST of Spg036511 vs. TAIR 10
Match: AT3G53570.3 (FUS3-complementing gene 1 )

HSP 1 Score: 647.1 bits (1668), Expect = 9.9e-186
Identity = 309/430 (71.86%), Postives = 357/430 (83.02%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPE-APKAQVGICCGQEIANMPSFASSRAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P   P           +   P FAS   P+ 
Sbjct: 3   LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 62

Query: 61  HSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDR 120
              N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQVLEC+D 
Sbjct: 63  VYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 122

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEK 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNHICIVFEK
Sbjct: 123 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 182

Query: 181 LGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVK 240
           LGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR+IHTDLKPENILLVS++Y+K
Sbjct: 183 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 242

Query: 241 VHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLG 300
           + DYK LSR  +D S FK +PKSSAIK+IDFGSTT++ QD NYIVSTRHYRAPEVILG+G
Sbjct: 243 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 302

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVR 360
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR +EKY R
Sbjct: 303 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 362

Query: 361 RG-RLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAR 420
           RG +LDWPEGATSRDS+KAV KLPRLPNLIMQHVDHSAG+LI L+QGLLRYDP  R  AR
Sbjct: 363 RGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAR 422

Query: 421 EALRHPFFTR 429
           EAL HPFFTR
Sbjct: 423 EALNHPFFTR 431

BLAST of Spg036511 vs. TAIR 10
Match: AT3G53570.1 (FUS3-complementing gene 1 )

HSP 1 Score: 647.1 bits (1668), Expect = 9.9e-186
Identity = 309/430 (71.86%), Postives = 357/430 (83.02%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPE-APKAQVGICCGQEIANMPSFASSRAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P   P           +   P FAS   P+ 
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 76

Query: 61  HSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDR 120
              N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQVLEC+D 
Sbjct: 77  VYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 136

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEK 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNHICIVFEK
Sbjct: 137 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 196

Query: 181 LGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVK 240
           LGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR+IHTDLKPENILLVS++Y+K
Sbjct: 197 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 256

Query: 241 VHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLG 300
           + DYK LSR  +D S FK +PKSSAIK+IDFGSTT++ QD NYIVSTRHYRAPEVILG+G
Sbjct: 257 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 316

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVR 360
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR +EKY R
Sbjct: 317 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 376

Query: 361 RG-RLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAR 420
           RG +LDWPEGATSRDS+KAV KLPRLPNLIMQHVDHSAG+LI L+QGLLRYDP  R  AR
Sbjct: 377 RGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAR 436

Query: 421 EALRHPFFTR 429
           EAL HPFFTR
Sbjct: 437 EALNHPFFTR 445

BLAST of Spg036511 vs. TAIR 10
Match: AT3G53570.2 (FUS3-complementing gene 1 )

HSP 1 Score: 647.1 bits (1668), Expect = 9.9e-186
Identity = 309/430 (71.86%), Postives = 357/430 (83.02%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPE-APKAQVGICCGQEIANMPSFASSRAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P   P           +   P FAS   P+ 
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 76

Query: 61  HSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDR 120
              N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQVLEC+D 
Sbjct: 77  VYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 136

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEK 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNHICIVFEK
Sbjct: 137 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 196

Query: 181 LGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVK 240
           LGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR+IHTDLKPENILLVS++Y+K
Sbjct: 197 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 256

Query: 241 VHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLG 300
           + DYK LSR  +D S FK +PKSSAIK+IDFGSTT++ QD NYIVSTRHYRAPEVILG+G
Sbjct: 257 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 316

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVR 360
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR +EKY R
Sbjct: 317 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 376

Query: 361 RG-RLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAR 420
           RG +LDWPEGATSRDS+KAV KLPRLPNLIMQHVDHSAG+LI L+QGLLRYDP  R  AR
Sbjct: 377 RGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAR 436

Query: 421 EALRHPFFTR 429
           EAL HPFFTR
Sbjct: 437 EALNHPFFTR 445

BLAST of Spg036511 vs. TAIR 10
Match: AT3G53570.4 (FUS3-complementing gene 1 )

HSP 1 Score: 647.1 bits (1668), Expect = 9.9e-186
Identity = 309/430 (71.86%), Postives = 357/430 (83.02%), Query Frame = 0

Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPE-APKAQVGICCGQEIANMPSFASSRAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P   P           +   P FAS   P+ 
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 76

Query: 61  HSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDR 120
              N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQVLEC+D 
Sbjct: 77  VYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 136

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEK 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+RCVQIRNWFDYRNHICIVFEK
Sbjct: 137 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 196

Query: 181 LGPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYVK 240
           LGPSLYDFLRKN+YRSFPIDLVR++GRQLLE VA+MHDLR+IHTDLKPENILLVS++Y+K
Sbjct: 197 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 256

Query: 241 VHDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLG 300
           + DYK LSR  +D S FK +PKSSAIK+IDFGSTT++ QD NYIVSTRHYRAPEVILG+G
Sbjct: 257 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 316

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVR 360
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR +EKY R
Sbjct: 317 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 376

Query: 361 RG-RLDWPEGATSRDSMKAVLKLPRLPNLIMQHVDHSAGELIHLVQGLLRYDPVHRLTAR 420
           RG +LDWPEGATSRDS+KAV KLPRLPNLIMQHVDHSAG+LI L+QGLLRYDP  R  AR
Sbjct: 377 RGAKLDWPEGATSRDSLKAVWKLPRLPNLIMQHVDHSAGDLIDLLQGLLRYDPTERFKAR 436

Query: 421 EALRHPFFTR 429
           EAL HPFFTR
Sbjct: 437 EALNHPFFTR 445

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894261.11.4e-25397.69serine/threonine-protein kinase AFC2 isoform X1 [Benincasa hispida][more]
XP_023519374.11.8e-25397.92serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita pepo subsp. pepo... [more]
XP_023001260.13.9e-25397.92serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita maxima][more]
XP_022923920.13.9e-25397.69serine/threonine-protein kinase AFC2 isoform X1 [Cucurbita moschata] >KAG6584116... [more]
XP_008439081.11.1e-25297.23PREDICTED: serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo] >KAA00... [more]
Match NameE-valueIdentityDescription
P515672.1e-22085.51Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=... [more]
P515661.4e-18471.86Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=... [more]
P515682.0e-14362.63Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=... [more]
P497601.1e-9147.21Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1[more]
O354911.8e-9148.71Dual specificity protein kinase CLK2 OS=Mus musculus OX=10090 GN=Clk2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1KKP81.9e-25397.92serine/threonine-protein kinase AFC2-like isoform X1 OS=Cucurbita maxima OX=3661... [more]
A0A6J1EDB41.9e-25397.69serine/threonine-protein kinase AFC2 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A5A7UP875.6e-25397.23Serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo var. makuwa OX=1... [more]
A0A6J1C5J15.6e-25397.46serine/threonine-protein kinase AFC2 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A1S3AXH45.6e-25397.23serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT4G24740.11.5e-22185.51FUS3-complementing gene 2 [more]
AT3G53570.39.9e-18671.86FUS3-complementing gene 1 [more]
AT3G53570.19.9e-18671.86FUS3-complementing gene 1 [more]
AT3G53570.29.9e-18671.86FUS3-complementing gene 1 [more]
AT3G53570.49.9e-18671.86FUS3-complementing gene 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 99..426
e-value: 1.6E-74
score: 263.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 99..426
e-value: 4.0E-49
score: 167.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 99..426
score: 37.318718
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 86..179
e-value: 7.5E-112
score: 375.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 153..426
e-value: 7.5E-112
score: 375.9
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 93..232
e-value: 3.4E-10
score: 37.2
NoneNo IPR availablePANTHERPTHR45646SERINE/THREONINE-PROTEIN KINASE DOA-RELATEDcoord: 1..433
NoneNo IPR availablePANTHERPTHR45646:SF16KINASE AFC1coord: 1..433
NoneNo IPR availableCDDcd14134PKc_CLKcoord: 86..426
e-value: 0.0
score: 558.334
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 220..232
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 86..429

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg036511.1Spg036511.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006397 mRNA processing
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity