Spg036496 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg036496
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptiontranscription elongation factor TFIIS-like
Locationscaffold5: 47369456 .. 47372801 (+)
RNA-Seq ExpressionSpg036496
SyntenySpg036496
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTTCCCTCCCAATAGCAAAAGCCGCTCCCAGCCTCTCTCCGTTCAACCACCGCCGCCGTTCAACCACCGTTGCTCGCACTTTCTTCGTGCATCGCAGCAGCCAAGCCAGCAAGGTGGATTTTCGTTTTACTTTCTCCTTTCTCTGTTTTTTTTTTTTTTTTTTGTTTGAAACTCTGGTTTTACTTTGTCATTTTTTTCCTTTTCAGTTTTCACGCCTCTTAAATCGAGATTTAAATTGAAACATTACTTCTGCTCCGCCGTCTCTCTGCCGAAATAAGGGATTTTTCCGAAGCCCATTCTGATTGGGTTTCCGCTTCTGTTTTCGATTTCTTCTATTTAGTGTGGGAATGCGAGCGTTTTTCCAGTCGTCAATTCAGATCTCTGTAAGTTTTACTCATTCGAAATCGATCAATTGCCATCGCGGGTGCGATGTCTTTTGATTCGATGGTATTCTTTTTCTTTTTCATTGAGAAAATAAGTCGAATTCGATTTTGATTGCGATAATCCCCCCTTTCTTTGTATGCGTCTGTTTGTATCTTTCCCTTTTATTGTTGTTGTTGGGATCTGATCATTCTTTTCTTCGAATGTGGTGGATAGGTTATATAAAAATTCCAAGTTGGCGGTGATTCGATGGAGAATGAGCTGGTGGAATTGTTTGAAGCGGCGAAGAAGGCTGCAGACGTCGCTGCAGCATCATCAAATGATGGGGGAGCCGAGGAAAGTCGTTGTCTAGATGCCCTACGGCAGCTCAAGAAATTTCCTGTTACTTATCAAATCCTTGTTTCCACTCAGGTTTTCTTCTCATTAGACTTGAAATTTGTTTCTTTTGTTGATGGTGGATGATAGTCAAAACGTAAAAAGCCGACTAGTTTGTAAAATCATACATCGTTTTTATTATGAATTAAGCCAAAACTCTTTGTTCTTTTTTTTTTTTGGCTTACGAATTGGTTTCATATCGAGCTGAGTTCACTATATTTCATCTGTTATTTGAGTTGTTCACATGACGAAATCATCTCACCTGTGACTCTTTAAGTTTTTGGAATAATACTCTGGTTGAATTCTTATAATTCCTTTTCCCGTGTGATTGAGGTTCATTTGCCTGTGTTGATAGTTTCGTTATCTTGTCCTCTGTAGGTTGGGAAGCGGCTTCGGCACCTCACTAAACACCCCAGGAAGAAAATACAGGACTATGCTTCTGACCTGATTGAGATGTGGAAGGACATAGTAATCAAGGAGACCAACAAGAATAAGAAAAATGGAAATGACAACTGTAAAGAGCGACCTAAAATTGGTTCTCCCAGTGCAGAGTCCGTTAAGGTGGAGAAGTTTCAAAAATCATCTTCCATGAAGGTTGAGAAAGTTTCCAAGGTTGAGCACTTCGATAGGAATGGTGCTACAAGCTCTGTAAAATTTTCCAGATCAGAAAGTGCCATCTCGGAGAAAAACTCTGTCAAGGTTGAGAAAACTGATTCAGTTGTCAAGGTTGAAAGAATAGTTAAAGAAGAGAAGAAAATCTCAATAGAAAAGAAACCATCAGGTGGGGTTGCTACACCTCCAAAGCTTACTTCCATGATTAAATCAAAAGATGCTGCTCGGGACAAAATTAGAGAACTTCTCTTTGAGGCCTTCTCCAAGGTTCCTGGCGAGGCTGATGAAGATGTTATAGATGAAGTAAATGCAAGTGATCCTATCCGTGTTGCTGTTTCTGTAGAATCCGTGATGTTTGAAAATTGGGGAGGTTCTACCGGAGCACAAAAGGCCAAGTACAGATCTATAATGTTTAACCTCAAGGATCCGAAGAACCCAGACTTTCGGAGAAAAGTTCTTCTTGGACTTATCAAGCCAGAAAGGGTGATCAACTTGAGCACAACTGATATGGCAAGTGATCAGAGAAAACGTGAGAATGAGGAGATTGCACAAAAGGCTTTATTTGATTGTGAGCGTGGAGGAGCTCCAAAAGCTACTACCGATCAGTTCAAGTGTGGTCGATGTGGTCAACGCAAGACAACCTACTATCAGTTGCAGACGCGGAGTGCTGATGAACCTATGACCACCTTTGTAACTTGTGTAAACTGCAATAACCATTGGAAGTTTTGTTGAGTTTCTCGTGAGCTACTGAAAATGTTAATTGTTTGGAACAATTTACTGAGCTGCTAGTGTTATTTTGGCTTGACACTGGTTGCAGTCTGTAATCTCGTTCCACATTGCTACATTTAGAACGTAGGAGGTTCTCTTATTGTAGGTTTTAAGTACCAATTTGTTGAGAAATTCGGTGTATAAACTTGTTTATTTGTTATTTCCTTCGGTATTTGTCAGAGTTTCATAAATCATACTTCCAAGGAACTTGTGTTTTCAGTAGTACTCGATTTGATCCTTTTGTCCTCTCAGGTTCATCTCACCCCCCCCCCCCCCCACACACATTGAACAGCACCCCAACTGTTGCATCCAGTAAAGTGCATAAGCGTGTAAATAAGGGAGTCTAATTTGTTGGTTTGATCATAAAACTGTCTAAAATTTATTAAAATCAAACCAACTGCTTATTGAAAAGTACAATCTTAGTACAAACCAACCAAATCAATTCAAAGCTTATGACTTCGAAACAAGCTTGCACCATTTATACAAAAACTAATATGATGTAGTTTTATTCTCTATGTTTTTTGTTTGAAGGAAAACAATGATAATACAACTAAATTAACTGGTAAAAATGAAGATCATGAGGCATGTGCTCGACGATAATGTGGCATATACCCCACTAGTCTTCGTCTTCGATACTAGACTATTATCTAGGAAACATGTTTCAGCTCTTTCACCCTCATATATGTTTCCCATTTTCCTTCCAAACGACACCCAAGAACTTGAAAATAAGCTAGACAGGAGCCTCTTGTTTACAGCCAGTACAAGATTGAAAGATCAAACAGGTTGAACCAGCATTTTTGTAAGGAGAAAGCTCCAAGTAGCCACATCTAGAGCGCCAATTTAAGACCTTAACACAAGAAGCGGTCCATAAAAAAGGAGCATCTTTGTGAGAACAATGAGCGTTTTTCATAAAATTGGGGGTGCGTTCCTAACTTGCTAGGCTGTAAAGTAACTAAATTATGTGTATTGTTAACCCGTACAGTTAGTAATCATGCCGTAGATGTATGACCATGAGAGGACGGAATAGGACTAGAGAATTGAAAGTAAGGTGGTGCAGTGGGGACCTTAAATGGAAGGGTCTACGCCTATCCAATTCCACTGCGATTGAGAGTGAAGTTGGGGGGCTGGGGCTGGGGCTGTGTGTGTGGCGTGGCGAGCCACTCTTCTTCAGTCAGGAATTTGTTCGAACATGTGGTGGGCTTGAATGA

mRNA sequence

ATGGAGAATGAGCTGGTGGAATTGTTTGAAGCGGCGAAGAAGGCTGCAGACGTCGCTGCAGCATCATCAAATGATGGGGGAGCCGAGGAAAGTCGTTGTCTAGATGCCCTACGGCAGCTCAAGAAATTTCCTGTTACTTATCAAATCCTTGTTTCCACTCAGGTTGGGAAGCGGCTTCGGCACCTCACTAAACACCCCAGGAAGAAAATACAGGACTATGCTTCTGACCTGATTGAGATGTGGAAGGACATAGTAATCAAGGAGACCAACAAGAATAAGAAAAATGGAAATGACAACTGTAAAGAGCGACCTAAAATTGGTTCTCCCAGTGCAGAGTCCGTTAAGGTGGAGAAGTTTCAAAAATCATCTTCCATGAAGGTTGAGAAAGTTTCCAAGGTTGAGCACTTCGATAGGAATGGTGCTACAAGCTCTGTAAAATTTTCCAGATCAGAAAGTGCCATCTCGGAGAAAAACTCTGTCAAGGTTGAGAAAACTGATTCAGTTGTCAAGGTTGAAAGAATAGTTAAAGAAGAGAAGAAAATCTCAATAGAAAAGAAACCATCAGGTGGGGTTGCTACACCTCCAAAGCTTACTTCCATGATTAAATCAAAAGATGCTGCTCGGGACAAAATTAGAGAACTTCTCTTTGAGGCCTTCTCCAAGGTTCCTGGCGAGGCTGATGAAGATGTTATAGATGAAGTAAATGCAAGTGATCCTATCCGTGTTGCTGTTTCTGTAGAATCCGTGATGTTTGAAAATTGGGGAGGTTCTACCGGAGCACAAAAGGCCAAGTACAGATCTATAATGTTTAACCTCAAGGATCCGAAGAACCCAGACTTTCGGAGAAAAGTTCTTCTTGGACTTATCAAGCCAGAAAGGGTGATCAACTTGAGCACAACTGATATGGCAAGACGGAATAGGACTAGAGAATTGAAAGTAAGGTGGTGCAGTGGGGACCTTAAATGGAAGGGTCTACGCCTATCCAATTCCACTGCGATTGAGAGTGAAGTTGGGGGGCTGGGGCTGGGGCTGTGTGTGTGGCGTGGCGAGCCACTCTTCTTCAGTCAGGAATTTGTTCGAACATGTGGTGGGCTTGAATGA

Coding sequence (CDS)

ATGGAGAATGAGCTGGTGGAATTGTTTGAAGCGGCGAAGAAGGCTGCAGACGTCGCTGCAGCATCATCAAATGATGGGGGAGCCGAGGAAAGTCGTTGTCTAGATGCCCTACGGCAGCTCAAGAAATTTCCTGTTACTTATCAAATCCTTGTTTCCACTCAGGTTGGGAAGCGGCTTCGGCACCTCACTAAACACCCCAGGAAGAAAATACAGGACTATGCTTCTGACCTGATTGAGATGTGGAAGGACATAGTAATCAAGGAGACCAACAAGAATAAGAAAAATGGAAATGACAACTGTAAAGAGCGACCTAAAATTGGTTCTCCCAGTGCAGAGTCCGTTAAGGTGGAGAAGTTTCAAAAATCATCTTCCATGAAGGTTGAGAAAGTTTCCAAGGTTGAGCACTTCGATAGGAATGGTGCTACAAGCTCTGTAAAATTTTCCAGATCAGAAAGTGCCATCTCGGAGAAAAACTCTGTCAAGGTTGAGAAAACTGATTCAGTTGTCAAGGTTGAAAGAATAGTTAAAGAAGAGAAGAAAATCTCAATAGAAAAGAAACCATCAGGTGGGGTTGCTACACCTCCAAAGCTTACTTCCATGATTAAATCAAAAGATGCTGCTCGGGACAAAATTAGAGAACTTCTCTTTGAGGCCTTCTCCAAGGTTCCTGGCGAGGCTGATGAAGATGTTATAGATGAAGTAAATGCAAGTGATCCTATCCGTGTTGCTGTTTCTGTAGAATCCGTGATGTTTGAAAATTGGGGAGGTTCTACCGGAGCACAAAAGGCCAAGTACAGATCTATAATGTTTAACCTCAAGGATCCGAAGAACCCAGACTTTCGGAGAAAAGTTCTTCTTGGACTTATCAAGCCAGAAAGGGTGATCAACTTGAGCACAACTGATATGGCAAGACGGAATAGGACTAGAGAATTGAAAGTAAGGTGGTGCAGTGGGGACCTTAAATGGAAGGGTCTACGCCTATCCAATTCCACTGCGATTGAGAGTGAAGTTGGGGGGCTGGGGCTGGGGCTGTGTGTGTGGCGTGGCGAGCCACTCTTCTTCAGTCAGGAATTTGTTCGAACATGTGGTGGGCTTGAATGA

Protein sequence

MENELVELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKKISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTTDMARRNRTRELKVRWCSGDLKWKGLRLSNSTAIESEVGGLGLGLCVWRGEPLFFSQEFVRTCGGLE
Homology
BLAST of Spg036496 vs. NCBI nr
Match: XP_038884917.1 (transcription elongation factor TFIIS [Benincasa hispida])

HSP 1 Score: 526.6 bits (1355), Expect = 1.8e-145
Identity = 276/310 (89.03%), Postives = 293/310 (94.52%), Query Frame = 0

Query: 1   MENELVELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELF+AAKKAAD AAA SNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFQAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQ 120
           HLTKHP+KKIQ+YASDL+EMWK+IVIKETNKNKKNGN NCK+ PKIGSPSAESVKVEKFQ
Sbjct: 61  HLTKHPKKKIQEYASDLVEMWKEIVIKETNKNKKNGNGNCKDSPKIGSPSAESVKVEKFQ 120

Query: 121 KSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKK 180
           KSSSMKVE+VSKVE FD+NGAT SVK+SR ESA+SE+NSVKVEKTDS VKVE  VKEE++
Sbjct: 121 KSSSMKVERVSKVEQFDKNGATGSVKYSRLESAVSERNSVKVEKTDSAVKVEGTVKEERR 180

Query: 181 ISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASDPI 240
           ISI+KKPSGG A PPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDV+DEVNASDPI
Sbjct: 181 ISIDKKPSGGTAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVMDEVNASDPI 240

Query: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTT 300
           RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPER+IN+ST 
Sbjct: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMINMSTA 300

Query: 301 DMARRNRTRE 311
           DMA   R RE
Sbjct: 301 DMASDQRKRE 310

BLAST of Spg036496 vs. NCBI nr
Match: XP_022957122.1 (transcription elongation factor TFIIS-like [Cucurbita moschata])

HSP 1 Score: 522.3 bits (1344), Expect = 3.3e-144
Identity = 284/310 (91.61%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MENELVELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAAD AAA SNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAARSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQ 120
           HLTKHP+KKIQDYASDLIEMWKDIVIKETNKNKKNGN N KE  KIGSPSAESVKVE  Q
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNSKEPSKIGSPSAESVKVENLQ 120

Query: 121 KSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKK 180
           KSSSMKVE+VSKVE FDRNG+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVER+VKEEKK
Sbjct: 121 KSSSMKVERVSKVEQFDRNGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERMVKEEKK 180

Query: 181 ISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASDPI 240
           ISIEKKPS G A PPKLTSMIKSKDAARDKIRELL EAFSKVPGEADEDVIDEVNASDPI
Sbjct: 181 ISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASDPI 240

Query: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTT 300
           RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPER+ NLSTT
Sbjct: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLSTT 300

Query: 301 DMARRNRTRE 311
           DMA   R RE
Sbjct: 301 DMASDQRKRE 309

BLAST of Spg036496 vs. NCBI nr
Match: KAG6602582.1 (Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 521.2 bits (1341), Expect = 7.4e-144
Identity = 283/310 (91.29%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MENELVELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAAD AAA SNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 300 MEKELVELFEAAKKAADAAAARSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 359

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQ 120
           HLTKHP+KKIQDYASDLIEMWKDIVIKETNKNKKNGN N KE  KIGSPSAESVKVE  Q
Sbjct: 360 HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNSKEPSKIGSPSAESVKVENLQ 419

Query: 121 KSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKK 180
           KSSSMKVE+VSKVE FDRNG+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVERIVKEEKK
Sbjct: 420 KSSSMKVERVSKVEQFDRNGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERIVKEEKK 479

Query: 181 ISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASDPI 240
           ISIEKKPS G A PPKLT+MIKSKDAARDKIRELL EAFSKVPGEADEDV+DEVNASDPI
Sbjct: 480 ISIEKKPS-GAAGPPKLTAMIKSKDAARDKIRELLVEAFSKVPGEADEDVMDEVNASDPI 539

Query: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTT 300
           RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPER+ NLSTT
Sbjct: 540 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLSTT 599

Query: 301 DMARRNRTRE 311
           DMA   R RE
Sbjct: 600 DMASDQRKRE 608

BLAST of Spg036496 vs. NCBI nr
Match: XP_023531955.1 (transcription elongation factor TFIIS-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 520.4 bits (1339), Expect = 1.3e-143
Identity = 282/310 (90.97%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MENELVELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAAD AAA SNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQ 120
           HLTKHP+KKIQDYASDLIEMWKDIVIKETNKNKKNGN N KE  KIGSPSAESVKVE  Q
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNSKETSKIGSPSAESVKVENLQ 120

Query: 121 KSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKK 180
           KSSSMKVE+VSKVE FDRNG+TSSVK+SRS SA+SEKNSVKVEKTDSVVKVER+VKEEKK
Sbjct: 121 KSSSMKVERVSKVEQFDRNGSTSSVKYSRSASAVSEKNSVKVEKTDSVVKVERMVKEEKK 180

Query: 181 ISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASDPI 240
           ISIEKKPS G A PPKLTSMIKSKDAARDKIRELL EAFSKVPGEADEDV+DEVNASDPI
Sbjct: 181 ISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVMDEVNASDPI 240

Query: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTT 300
           RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPER+ NLSTT
Sbjct: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLSTT 300

Query: 301 DMARRNRTRE 311
           DMA   R RE
Sbjct: 301 DMASDQRKRE 309

BLAST of Spg036496 vs. NCBI nr
Match: XP_022989946.1 (transcription elongation factor TFIIS-like [Cucurbita maxima])

HSP 1 Score: 519.2 bits (1336), Expect = 2.8e-143
Identity = 283/310 (91.29%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MENELVELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAAD AAA SNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQ 120
           HLTKHP+KKIQDYASDLIEMWKDIVIKETNKNKKNGN N KE  KIGSPSAESVKVE FQ
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNSKEPSKIGSPSAESVKVENFQ 120

Query: 121 KSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKK 180
           KSSSMKVE+VSKVE F+R G+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVER+VKEEKK
Sbjct: 121 KSSSMKVERVSKVEQFNRIGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERMVKEEKK 180

Query: 181 ISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASDPI 240
           ISIEKKPS G A PPKLTSMIKSKDAARDKIRELL EAFSKVPGEADEDVIDEVNASDPI
Sbjct: 181 ISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASDPI 240

Query: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTT 300
           RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPER+ NLSTT
Sbjct: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLSTT 300

Query: 301 DMARRNRTRE 311
           DMA   R RE
Sbjct: 301 DMASDQRKRE 309

BLAST of Spg036496 vs. ExPASy Swiss-Prot
Match: Q9ZVH8 (Transcription elongation factor TFIIS OS=Arabidopsis thaliana OX=3702 GN=TFIIS PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 9.6e-70
Identity = 163/317 (51.42%), Postives = 206/317 (64.98%), Query Frame = 0

Query: 1   MENELVELFEAAKKAADVAAASS-NDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRL 60
           ME++L++LFE AKKAAD AA       G E S+C+DAL+QLKKFPVTY  LV+TQVGK+L
Sbjct: 1   MESDLIDLFEGAKKAADAAALDGVTSSGPEVSQCIDALKQLKKFPVTYDTLVATQVGKKL 60

Query: 61  RHLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKK-NGNDNCKE----RPKIGSPSAES- 120
           R L KHP + I+  A+DL+E+WK +VI+ET K KK  G + CKE    +  +  PS  + 
Sbjct: 61  RSLAKHPVEDIKSVATDLLEIWKKVVIEETAKAKKTEGTNGCKEAKVNKMDVEKPSNPAP 120

Query: 121 VKVEKFQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVER 180
           VKV+K Q+  S K  KV + E    N   + VK  R    I   N  K++     VK E+
Sbjct: 121 VKVQKLQRGDSAKSIKVERKE--PDNKVVTGVKIERKVPDIKVTNGTKIDYRGQAVKDEK 180

Query: 181 IVKEEKKISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDE 240
           + K+ +  S  K P+     PPKLT+M+K  D  RDKIRELL EA  +V GEAD+   + 
Sbjct: 181 VSKDNQ--SSMKAPAKAANAPPKLTAMLKCNDPVRDKIRELLVEALCRVAGEADDYERES 240

Query: 241 VNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPER 300
           VNASDP+RVAVSVES+MFE  G STGAQK KYRSIMFNL+D  NPD RR+VL G I PE+
Sbjct: 241 VNASDPLRVAVSVESLMFEKLGRSTGAQKLKYRSIMFNLRDSNNPDLRRRVLTGEISPEK 300

Query: 301 VINLSTTDMARRNRTRE 311
           +I LS  DMA   R +E
Sbjct: 301 LITLSAEDMASDKRKQE 313

BLAST of Spg036496 vs. ExPASy Swiss-Prot
Match: Q2KI09 (Transcription elongation factor A protein 3 OS=Bos taurus OX=9913 GN=TCEA3 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.1e-08
Identity = 79/315 (25.08%), Postives = 135/315 (42.86%), Query Frame = 0

Query: 7   ELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKH- 66
           EL   AKK   + A    +G       LD L++L  + ++ Q+L +T++G  +  + KH 
Sbjct: 6   ELLRIAKKLEKMVARKKTEG------ALDLLKKLGSWQMSIQLLQTTRIGVAVNGVRKHC 65

Query: 67  PRKKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQKSSSM 126
             K++   A  LI  WK ++        + G +  K + K      + +    ++  +S+
Sbjct: 66  SNKEVVALAKVLIRNWKQLLDSPATPKGEKGEERVKAKKK-----EKGLDCSDWKPETSL 125

Query: 127 KVEKVSKVEH--------FDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKE 186
              +  +VE           ++ ATSS K    E + S K+  +  +T S          
Sbjct: 126 SPPRKKRVEEPKDRRDSVDSKSSATSSPKRPSMERSNSSKSKAETPRTPS---------- 185

Query: 187 EKKISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNAS 246
               S    PS  +  P  LT      D+ RDK  E+L  A      +AD+D  D     
Sbjct: 186 -SPSSPTFAPSVCLLAPCYLTG-----DSVRDKCVEMLSAAL-----KADDDYKDYGVNC 245

Query: 247 DPIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINL 306
           D  ++A  +E  +++    +    + + RS + NLKDP+NP  RR VL G I    +  +
Sbjct: 246 D--KMASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISAGLIAKM 285

Query: 307 STTDMARRNRTRELK 313
           +  +MA  +  REL+
Sbjct: 306 TAEEMA-SDELRELR 285

BLAST of Spg036496 vs. ExPASy Swiss-Prot
Match: P23881 (Transcription elongation factor A protein 3 OS=Mus musculus OX=10090 GN=Tcea3 PE=1 SV=3)

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-07
Identity = 76/308 (24.68%), Postives = 131/308 (42.53%), Query Frame = 0

Query: 7   ELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKH- 66
           EL   AKK   + +    +G       LD L++L    ++ Q+L +T++G  +  + KH 
Sbjct: 6   ELLRIAKKLEKMVSRKKTEG------ALDLLKKLNSCQMSIQLLQTTRIGVAVNGVRKHC 65

Query: 67  PRKKIQDYASDLIEMWKDIV-IKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQKSSS 126
             K++   A  LI+ WK ++    T K ++   +  K+   +G    +        +   
Sbjct: 66  SDKEVVSLAKVLIKNWKRLLDSPRTTKGEREEREKAKKEKGLGCSDWKPEAGLSPPRKKG 125

Query: 127 MKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKKISIE 186
               K  +     R+  TSS K    E + S K+ V+   T S                 
Sbjct: 126 GGEPKTRRDSVDSRSSTTSSPKRPSLERSNSSKSKVETPTTPSSPSTPTFA--------- 185

Query: 187 KKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASDPIRVAV 246
             P+  +  P  LT      D+ RDK  E+L  A      +A+++  D     D  ++A 
Sbjct: 186 --PAVCLLAPCYLTG-----DSVRDKCVEMLSAAL-----KAEDNFKDYGVNCD--KLAS 245

Query: 247 SVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTTDMAR 306
            +E  +++    +    + + RS + NLKDP+NP  RR VL G I PE +  ++  +MA 
Sbjct: 246 EIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISPELIAKMTAEEMA- 283

Query: 307 RNRTRELK 313
            +  REL+
Sbjct: 306 SDELRELR 283

BLAST of Spg036496 vs. ExPASy Swiss-Prot
Match: Q92576 (PHD finger protein 3 OS=Homo sapiens OX=9606 GN=PHF3 PE=1 SV=3)

HSP 1 Score: 57.8 bits (138), Expect = 3.0e-07
Identity = 49/157 (31.21%), Postives = 77/157 (49.04%), Query Frame = 0

Query: 155  SEKNSVKVEKTDSVV-----KVERIVKEEKKISIEKKPSGGVA-TPPKLTSMIKSKDAAR 214
            SE+ S K+ K  + V     K  +    EK+   +KK   GV    P  ++   S D  R
Sbjct: 869  SEEKSEKIPKESTTVTCTGEKASKPGTHEKQEMKKKKVEKGVLNVHPAASASKPSADQIR 928

Query: 215  DKIRELLFEAFSKVPGEADEDVIDEVNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSI 274
              +R  L +   K   +++  V +E  A    +VA  +E  +F  +  +    K KYRS+
Sbjct: 929  QSVRHSLKDILMKRLTDSNLKVPEEKAA----KVATKIEKELFSFFRDTDAKYKNKYRSL 988

Query: 275  MFNLKDPKNPDFRRKVLLGLIKPERVINLSTTDMARR 306
            MFNLKDPKN    +KVL G + P+ +I +S  ++A +
Sbjct: 989  MFNLKDPKNNILFKKVLKGEVTPDHLIRMSPEELASK 1021

BLAST of Spg036496 vs. ExPASy Swiss-Prot
Match: P20232 (Transcription elongation factor S-II OS=Drosophila melanogaster OX=7227 GN=TfIIS PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.2e-06
Identity = 71/298 (23.83%), Postives = 114/298 (38.26%), Query Frame = 0

Query: 7   ELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLRHLTKHP 66
           E+F   KK + +A+      G  + + LD L+ L+   +   IL  T++G  +  L K  
Sbjct: 6   EVFRIQKKMSKMAS-----DGTGQDQALDLLKALQTLNINLDILTKTRIGMTVNELRKSS 65

Query: 67  R-KKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQKSSSM 126
           +  ++   A  LI+ WK  +           N + KE     S +++S    K   S S 
Sbjct: 66  KDDEVIALAKTLIKNWKRFLASPAPTTP--NNSSAKEGSSNNSSASKSTSAAKSSSSISG 125

Query: 127 KVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKKISIEK 186
           K                      +S S+ S K+  K   T S              S   
Sbjct: 126 K---------------------DKSSSSSSSKDKEKKGSTSS--------------SQTS 185

Query: 187 KPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASDPIRVAVS 246
            PSGG+             DA R K RE+L  A     GE  E         +P  +A  
Sbjct: 186 FPSGGMT------------DAVRIKCREMLATALK--IGEVPE------GCGEPEEMAAE 241

Query: 247 VESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTTDMA 304
           +E  ++  +  +    K + RS + NLKDPKNP  R   + G +  +++  ++  +MA
Sbjct: 246 LEDAIYSEFNNTDMKYKNRIRSRVANLKDPKNPGLRGNFMCGAVTAKQLAKMTPEEMA 241

BLAST of Spg036496 vs. ExPASy TrEMBL
Match: A0A6J1GYA3 (transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC111458597 PE=4 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.6e-144
Identity = 284/310 (91.61%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MENELVELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAAD AAA SNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAARSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQ 120
           HLTKHP+KKIQDYASDLIEMWKDIVIKETNKNKKNGN N KE  KIGSPSAESVKVE  Q
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNSKEPSKIGSPSAESVKVENLQ 120

Query: 121 KSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKK 180
           KSSSMKVE+VSKVE FDRNG+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVER+VKEEKK
Sbjct: 121 KSSSMKVERVSKVEQFDRNGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERMVKEEKK 180

Query: 181 ISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASDPI 240
           ISIEKKPS G A PPKLTSMIKSKDAARDKIRELL EAFSKVPGEADEDVIDEVNASDPI
Sbjct: 181 ISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASDPI 240

Query: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTT 300
           RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPER+ NLSTT
Sbjct: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLSTT 300

Query: 301 DMARRNRTRE 311
           DMA   R RE
Sbjct: 301 DMASDQRKRE 309

BLAST of Spg036496 vs. ExPASy TrEMBL
Match: A0A6J1JLK7 (transcription elongation factor TFIIS-like OS=Cucurbita maxima OX=3661 GN=LOC111486982 PE=4 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.4e-143
Identity = 283/310 (91.29%), Postives = 290/310 (93.55%), Query Frame = 0

Query: 1   MENELVELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAAD AAA SNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQ 120
           HLTKHP+KKIQDYASDLIEMWKDIVIKETNKNKKNGN N KE  KIGSPSAESVKVE FQ
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNSKEPSKIGSPSAESVKVENFQ 120

Query: 121 KSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKK 180
           KSSSMKVE+VSKVE F+R G+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVER+VKEEKK
Sbjct: 121 KSSSMKVERVSKVEQFNRIGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERMVKEEKK 180

Query: 181 ISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASDPI 240
           ISIEKKPS G A PPKLTSMIKSKDAARDKIRELL EAFSKVPGEADEDVIDEVNASDPI
Sbjct: 181 ISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASDPI 240

Query: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTT 300
           RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPER+ NLSTT
Sbjct: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLSTT 300

Query: 301 DMARRNRTRE 311
           DMA   R RE
Sbjct: 301 DMASDQRKRE 309

BLAST of Spg036496 vs. ExPASy TrEMBL
Match: A0A6J1BWY6 (transcription elongation factor TFIIS OS=Momordica charantia OX=3673 GN=LOC111006416 PE=4 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.2e-142
Identity = 276/312 (88.46%), Postives = 289/312 (92.63%), Query Frame = 0

Query: 1   MENELVELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFEAAKKAAD AAA SNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQ 120
           HLTKHP+KKIQDYASDLIEMWK+IVIKETNKNKKNGN NCKE PKIGSPSAESVKVEKFQ
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNGNCKESPKIGSPSAESVKVEKFQ 120

Query: 121 KSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSV--VKVERIVKEE 180
           KSSSMKVE+VSKVE  DRNG TSSVK+SRSES ISEKN VKVEKTDS+  VK ER+VKEE
Sbjct: 121 KSSSMKVERVSKVEQSDRNGTTSSVKYSRSESVISEKNIVKVEKTDSLEKVKAERVVKEE 180

Query: 181 KKISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASD 240
           KKISI+K+PS G A PPKLTSMIKSKDAARDKIRELL EAFSKVPGEADEDVIDEVNASD
Sbjct: 181 KKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLS 300
           P+RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRR VL+G IKPER+IN+S
Sbjct: 241 PVRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLINMS 300

Query: 301 TTDMARRNRTRE 311
           T +MA   R RE
Sbjct: 301 TAEMASDQRKRE 312

BLAST of Spg036496 vs. ExPASy TrEMBL
Match: A0A6J1FF11 (transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC111444888 PE=4 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 3.4e-142
Identity = 274/310 (88.39%), Postives = 289/310 (93.23%), Query Frame = 0

Query: 1   MENELVELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFEAAKKAAD AAA SNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQ 120
           HLTKHP+KKIQ+YASDLIE+WK+IVIK+TNKNKKNGN +C+E PKIGSPSAESVKVEKF+
Sbjct: 61  HLTKHPKKKIQNYASDLIEIWKEIVIKDTNKNKKNGNIDCEESPKIGSPSAESVKVEKFR 120

Query: 121 KSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKK 180
           KSSSMKVE+VS+VE FDRNG TSS KFSRSES IS KN VKVEKTDSVVKVERIVKEEK+
Sbjct: 121 KSSSMKVERVSQVEQFDRNGVTSSAKFSRSESDISGKNPVKVEKTDSVVKVERIVKEEKR 180

Query: 181 ISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASDPI 240
           ISIEKKPS G   PPKLTSMIKSKDAARDKIRELLFEAFSKVP EADEDV+DEVNASDPI
Sbjct: 181 ISIEKKPSSGPDAPPKLTSMIKSKDAARDKIRELLFEAFSKVPSEADEDVMDEVNASDPI 240

Query: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTT 300
           RVAVSVESVMFENWGGSTG+QK KYRSIMFNLKDPKNPDFRRKVLLGLIKPER+IN+ST 
Sbjct: 241 RVAVSVESVMFENWGGSTGSQKTKYRSIMFNLKDPKNPDFRRKVLLGLIKPERLINMSTA 300

Query: 301 DMARRNRTRE 311
           DMA   R RE
Sbjct: 301 DMASDQRKRE 310

BLAST of Spg036496 vs. ExPASy TrEMBL
Match: A0A0A0KDH9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014610 PE=4 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.4e-140
Identity = 271/310 (87.42%), Postives = 288/310 (92.90%), Query Frame = 0

Query: 1   MENELVELFEAAKKAADVAAASSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60
           MENELVELFEAAKKAAD AAA SNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNDNCKERPKIGSPSAESVKVEKFQ 120
           HLTKHP+KKIQ++ASDLIEMWK+IVIKETNKNKKNGN + K+ PKIGSPSAESVKVEKFQ
Sbjct: 61  HLTKHPKKKIQEHASDLIEMWKEIVIKETNKNKKNGNASSKDSPKIGSPSAESVKVEKFQ 120

Query: 121 KSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKK 180
           KSSSMKVE+VSKVE FDRNGATSSVK+S+SES +SE+NSVKVEKTDS+VKVER+VKE   
Sbjct: 121 KSSSMKVERVSKVEQFDRNGATSSVKYSKSESVVSERNSVKVEKTDSMVKVERVVKE--- 180

Query: 181 ISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDEVNASDPI 240
              EKKPS G A PPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADE+ +DEVNASDPI
Sbjct: 181 ---EKKPSSGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEEFMDEVNASDPI 240

Query: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTT 300
           RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPER+IN+ST 
Sbjct: 241 RVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMINMSTA 300

Query: 301 DMARRNRTRE 311
           DMA   R RE
Sbjct: 301 DMASDQRKRE 304

BLAST of Spg036496 vs. TAIR 10
Match: AT2G38560.1 (transcript elongation factor IIS )

HSP 1 Score: 265.4 bits (677), Expect = 6.8e-71
Identity = 163/317 (51.42%), Postives = 206/317 (64.98%), Query Frame = 0

Query: 1   MENELVELFEAAKKAADVAAASS-NDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRL 60
           ME++L++LFE AKKAAD AA       G E S+C+DAL+QLKKFPVTY  LV+TQVGK+L
Sbjct: 1   MESDLIDLFEGAKKAADAAALDGVTSSGPEVSQCIDALKQLKKFPVTYDTLVATQVGKKL 60

Query: 61  RHLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKK-NGNDNCKE----RPKIGSPSAES- 120
           R L KHP + I+  A+DL+E+WK +VI+ET K KK  G + CKE    +  +  PS  + 
Sbjct: 61  RSLAKHPVEDIKSVATDLLEIWKKVVIEETAKAKKTEGTNGCKEAKVNKMDVEKPSNPAP 120

Query: 121 VKVEKFQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVER 180
           VKV+K Q+  S K  KV + E    N   + VK  R    I   N  K++     VK E+
Sbjct: 121 VKVQKLQRGDSAKSIKVERKE--PDNKVVTGVKIERKVPDIKVTNGTKIDYRGQAVKDEK 180

Query: 181 IVKEEKKISIEKKPSGGVATPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDE 240
           + K+ +  S  K P+     PPKLT+M+K  D  RDKIRELL EA  +V GEAD+   + 
Sbjct: 181 VSKDNQ--SSMKAPAKAANAPPKLTAMLKCNDPVRDKIRELLVEALCRVAGEADDYERES 240

Query: 241 VNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPER 300
           VNASDP+RVAVSVES+MFE  G STGAQK KYRSIMFNL+D  NPD RR+VL G I PE+
Sbjct: 241 VNASDPLRVAVSVESLMFEKLGRSTGAQKLKYRSIMFNLRDSNNPDLRRRVLTGEISPEK 300

Query: 301 VINLSTTDMARRNRTRE 311
           +I LS  DMA   R +E
Sbjct: 301 LITLSAEDMASDKRKQE 313

BLAST of Spg036496 vs. TAIR 10
Match: AT4G18720.1 (Transcription factor IIS protein )

HSP 1 Score: 98.6 bits (244), Expect = 1.1e-20
Identity = 55/109 (50.46%), Postives = 71/109 (65.14%), Query Frame = 0

Query: 195 PKLTSMIKSKDAARDKIRELLFEAFSKVPGE-ADEDVIDEVNASDPIRVAVSVESVMFEN 254
           P   +M K+ D+ RDK+RE+L  + +KV  E  D ++   V A DP  VAVSVE+ MFEN
Sbjct: 105 PTHATMKKTGDSKRDKVREILQTSLAKVASEVVDTEMKTRVTACDPWVVAVSVETAMFEN 164

Query: 255 WGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTTDM 303
            G   G QKAKYRSI+FN+ D  NPD RRKVLLG I  ER++ +   +M
Sbjct: 165 LGCFMGPQKAKYRSILFNMGDSNNPDLRRKVLLGEISGERLVKMEKEEM 213

BLAST of Spg036496 vs. TAIR 10
Match: AT2G42730.1 (F-box family protein )

HSP 1 Score: 95.1 bits (235), Expect = 1.2e-19
Identity = 54/119 (45.38%), Postives = 73/119 (61.34%), Query Frame = 0

Query: 195 PKLTSMIKSKDAARDKIRELLFEAFSKVPGE-ADEDVIDEVNASDPIRVAVSVESVMFEN 254
           P  ++M K+ D+ RDK+RE+L  +  KV  E  D ++   V A DP  VAVSVES MFE 
Sbjct: 594 PTHSTMKKTGDSKRDKVREILQTSLVKVASEIVDTEMKTRVTACDPSVVAVSVESAMFEK 653

Query: 255 WGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTTDMARRNRTRELK 313
            G   G  KAKYRSI+FN+ D  NPD RRKVL+G I  ER++ +   +M      +E++
Sbjct: 654 LGCFMGPHKAKYRSILFNMGDSNNPDLRRKVLIGEINGERLVTMERQEMGSEKIQKEVQ 712

BLAST of Spg036496 vs. TAIR 10
Match: AT5G42325.1 (Transcription factor IIS protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.5e-09
Identity = 41/120 (34.17%), Postives = 62/120 (51.67%), Query Frame = 0

Query: 195 PKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVIDE--VNASDPIRVAVSVESVMFE 254
           P  ++M K+ D+ RDK+ E+L  + +KV  E  +  +    +   DP  VAVSVES M  
Sbjct: 104 PTHSTMKKTGDSKRDKVHEILQSSLAKVATEVVDTEMKRRVMTVCDPWVVAVSVESAM-- 163

Query: 255 NWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERVINLSTTDMARRNRTRELK 313
                         SI+FN+ D  NPD RRKVL+G I  ER++ +   +M      +E++
Sbjct: 164 --------------SILFNMGDSNNPDLRRKVLIGEISGERLVKMEKDEMGSEKIQKEVQ 207

BLAST of Spg036496 vs. TAIR 10
Match: AT2G33090.1 (Transcription elongation factor (TFIIS) family protein )

HSP 1 Score: 58.5 bits (140), Expect = 1.3e-08
Identity = 34/97 (35.05%), Postives = 58/97 (59.79%), Query Frame = 0

Query: 2   ENELVELFEAAKKAADVAAASS-NDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 61
           + + +ELFE AKK+AD A A        E S C+D L  L +FP++ + + + ++  RL 
Sbjct: 151 DTDTLELFEIAKKSADFANAKGILASEVETSMCVDTLTLLLEFPISARDMETRKLMVRLE 210

Query: 62  HLTKHPRKKIQDYASDLIEMWKDIV----IKETNKNK 94
           HLTKH  +KI + AS L++ W++ +    ++E+ K +
Sbjct: 211 HLTKHKNRKICNSASKLLQCWRESIREQQLRESRKTQ 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884917.11.8e-14589.03transcription elongation factor TFIIS [Benincasa hispida][more]
XP_022957122.13.3e-14491.61transcription elongation factor TFIIS-like [Cucurbita moschata][more]
KAG6602582.17.4e-14491.29Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023531955.11.3e-14390.97transcription elongation factor TFIIS-like [Cucurbita pepo subsp. pepo][more]
XP_022989946.12.8e-14391.29transcription elongation factor TFIIS-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9ZVH89.6e-7051.42Transcription elongation factor TFIIS OS=Arabidopsis thaliana OX=3702 GN=TFIIS P... [more]
Q2KI092.1e-0825.08Transcription elongation factor A protein 3 OS=Bos taurus OX=9913 GN=TCEA3 PE=2 ... [more]
P238811.4e-0724.68Transcription elongation factor A protein 3 OS=Mus musculus OX=10090 GN=Tcea3 PE... [more]
Q925763.0e-0731.21PHD finger protein 3 OS=Homo sapiens OX=9606 GN=PHF3 PE=1 SV=3[more]
P202321.2e-0623.83Transcription elongation factor S-II OS=Drosophila melanogaster OX=7227 GN=TfIIS... [more]
Match NameE-valueIdentityDescription
A0A6J1GYA31.6e-14491.61transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1JLK71.4e-14391.29transcription elongation factor TFIIS-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1BWY61.2e-14288.46transcription elongation factor TFIIS OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A6J1FF113.4e-14288.39transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A0A0KDH92.4e-14087.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014610 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G38560.16.8e-7151.42transcript elongation factor IIS [more]
AT4G18720.11.1e-2050.46Transcription factor IIS protein [more]
AT2G42730.11.2e-1945.38F-box family protein [more]
AT5G42325.11.5e-0934.17Transcription factor IIS protein [more]
AT2G33090.11.3e-0835.05Transcription elongation factor (TFIIS) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003618Transcription elongation factor S-II, central domainSMARTSM00510mid_6coord: 205..310
e-value: 5.8E-29
score: 112.2
IPR003618Transcription elongation factor S-II, central domainPFAMPF07500TFIIS_Mcoord: 205..308
e-value: 1.6E-24
score: 86.6
IPR003618Transcription elongation factor S-II, central domainPROSITEPS51321TFIIS_CENTRALcoord: 207..303
score: 21.151768
IPR003617Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-typeSMARTSM00509TFS2_5coord: 8..87
e-value: 2.5E-23
score: 93.5
IPR035100Transcription elongation factor, IIS-typePIRSFPIRSF006704TFIIScoord: 2..314
e-value: 2.4E-49
score: 166.1
IPR017923Transcription factor IIS, N-terminalPFAMPF08711Med26coord: 36..85
e-value: 3.3E-11
score: 43.0
IPR017923Transcription factor IIS, N-terminalPROSITEPS51319TFIIS_Ncoord: 10..88
score: 20.486874
IPR035441TFIIS/LEDGF domain superfamilyGENE3D1.20.930.10Conserved domain common to transcription factors TFIIS, elongin A, CRSP70coord: 1..100
e-value: 2.6E-23
score: 83.6
IPR035441TFIIS/LEDGF domain superfamilySUPERFAMILY47676Conserved domain common to transcription factors TFIIS, elongin A, CRSP70coord: 28..98
IPR036575Transcription elongation factor S-II, central domain superfamilyGENE3D1.10.472.30coord: 184..304
e-value: 1.9E-24
score: 88.0
IPR036575Transcription elongation factor S-II, central domain superfamilySUPERFAMILY46942Elongation factor TFIIS domain 2coord: 195..304
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 91..112
NoneNo IPR availablePANTHERPTHR11477:SF36TRANSCRIPTION ELONGATION FACTOR TFIIScoord: 1..311
NoneNo IPR availablePANTHERPTHR11477TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEINcoord: 1..311
NoneNo IPR availableCDDcd00183TFIIS_Icoord: 22..85
e-value: 1.69913E-15
score: 68.8742

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg036496.1Spg036496.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0006414 translational elongation
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003746 translation elongation factor activity
molecular_function GO:0008270 zinc ion binding