Spg036426 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg036426
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	kunitz trypsin inhibitor 2
Location	scaffold5: 46238338 .. 46238952 (+)
RNA-Seq Expression	Spg036426
Synteny	Spg036426

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAATTTCGCATTACTGTCTTTTCTTTTCATTCTCCTCGCCTCCACTGAGGTCCGCTTCTGCAGAGCCGACGCCTCGCCGGACGCCGTCCTCGACATCGACGGCAAGAAGCTCCGCGCCGGCGTCAACTACTACATCCTCCCTGTTATCCGCGGAAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCCGACAAATGTCCACTCAACGTCGTTCAAGAACAATTCGAGATAATGAACGGCCTTCCTGCAACATTCGCGCCTGTAAACCCTAAAAAGGGCGTCGTTCGAGTTTCGACCGATTTGAACGTCCAATTCGAGGCCAGCACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACGGGACAGTGGTTCGTGACGATCGGCGGAACGAAAGGAAATCCAGGCGTCGAGACGGTGGACAACTGGTTCAAAATTGAGAAGCACGGTAACGATTATAAGTTCGTTTTCTGTCCGACTGTGTGCGATTTCTGTAAAGTTATGTGCAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTCTGGCTTTGAGCGATTCGCCATTCCCTGTTATGTTCAAGAAAGTTTGA

mRNA sequence

ATGAAGAATTTCGCATTACTGTCTTTTCTTTTCATTCTCCTCGCCTCCACTGAGGTCCGCTTCTGCAGAGCCGACGCCTCGCCGGACGCCGTCCTCGACATCGACGGCAAGAAGCTCCGCGCCGGCGTCAACTACTACATCCTCCCTGTTATCCGCGGAAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCCGACAAATGTCCACTCAACGTCGTTCAAGAACAATTCGAGATAATGAACGGCCTTCCTGCAACATTCGCGCCTGTAAACCCTAAAAAGGGCGTCGTTCGAGTTTCGACCGATTTGAACGTCCAATTCGAGGCCAGCACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACGGGACAGTGGTTCGTGACGATCGGCGGAACGAAAGGAAATCCAGGCGTCGAGACGGTGGACAACTGGTTCAAAATTGAGAAGCACGGTAACGATTATAAGTTCGTTTTCTGTCCGACTGTGTGCGATTTCTGTAAAGTTATGTGCAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTCTGGCTTTGAGCGATTCGCCATTCCCTGTTATGTTCAAGAAAGTTTGA

Coding sequence (CDS)

ATGAAGAATTTCGCATTACTGTCTTTTCTTTTCATTCTCCTCGCCTCCACTGAGGTCCGCTTCTGCAGAGCCGACGCCTCGCCGGACGCCGTCCTCGACATCGACGGCAAGAAGCTCCGCGCCGGCGTCAACTACTACATCCTCCCTGTTATCCGCGGAAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCCGACAAATGTCCACTCAACGTCGTTCAAGAACAATTCGAGATAATGAACGGCCTTCCTGCAACATTCGCGCCTGTAAACCCTAAAAAGGGCGTCGTTCGAGTTTCGACCGATTTGAACGTCCAATTCGAGGCCAGCACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACGGGACAGTGGTTCGTGACGATCGGCGGAACGAAAGGAAATCCAGGCGTCGAGACGGTGGACAACTGGTTCAAAATTGAGAAGCACGGTAACGATTATAAGTTCGTTTTCTGTCCGACTGTGTGCGATTTCTGTAAAGTTATGTGCAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTCTGGCTTTGAGCGATTCGCCATTCCCTGTTATGTTCAAGAAAGTTTGA

Protein sequence

MKNFALLSFLFILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTLGNLQSDKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRDIGIFFKNGKRALALSDSPFPVMFKKV

Homology

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BLAST of Spg036426 vs. NCBI nr
Match: XP_022923703.1 (kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin inhibitor 2 [Cucurbita maxima] >KAG6584289.1 Kunitz trypsin inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019882.1 Kunitz trypsin inhibitor 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 390.2 bits (1001), Expect = 1.1e-104
Identity = 187/204 (91.67%), Postives = 194/204 (95.10%), Query Frame = 0


Query: 1   MKNFALLSFLFILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTL 60
           MKNFALLSFLFILLAS EVR  RADASPDAV DIDGKKLRAGVNYYILPV RGRGGGL L
Sbjct: 1   MKNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLAL 60

Query: 61  GNLQSDKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTVWK 120
           GNLQSDKCPLNVVQEQFE+MNGLPA F PVNPKKGVVRVSTDLNVQFEASTICATSTVWK
Sbjct: 61  GNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWK 120

Query: 121 LDKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRDIG 180
           LDKFDEST QWF+TIGGT+GNPGV+TVDNWFKIEKHGNDYKF FCPTVCDFCKVMCRD+G
Sbjct: 121 LDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVG 180

Query: 181 IFFKNGKRALALSDSPFPVMFKKV 205
           IFFKNGKRALALSD+PFPVMFK+V
Sbjct: 181 IFFKNGKRALALSDTPFPVMFKEV 203

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BLAST of Spg036426 vs. NCBI nr
Match: XP_023520252.1 (kunitz trypsin inhibitor 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 387.1 bits (993), Expect = 9.3e-104
Identity = 186/204 (91.18%), Postives = 192/204 (94.12%), Query Frame = 0


Query: 1   MKNFALLSFLFILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTL 60
           MKNFALLSFLFILLAS EVR  RADASPDAV DIDGKKLRAGVNYYILPV RGRGGGL L
Sbjct: 1   MKNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLAL 60

Query: 61  GNLQSDKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTVWK 120
           GNLQSDKCPLNVVQEQFE+MNGLPA F PVNPKKGVVRVSTDLNVQFEASTICATSTVWK
Sbjct: 61  GNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWK 120

Query: 121 LDKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRDIG 180
           LDKFDEST QWF+TIGG +GNPGV+TVDNWFKIEKHGNDYKF FCPTVCDFCKVMCRD+G
Sbjct: 121 LDKFDESTKQWFITIGGARGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVG 180

Query: 181 IFFKNGKRALALSDSPFPVMFKKV 205
           IFFKNGKRALALSD+PFPVMFK V
Sbjct: 181 IFFKNGKRALALSDTPFPVMFKVV 203

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BLAST of Spg036426 vs. NCBI nr
Match: XP_038895203.1 (kunitz trypsin inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 383.6 bits (984), Expect = 1.0e-102
Identity = 184/205 (89.76%), Postives = 192/205 (93.66%), Query Frame = 0


Query: 1   MKNFA-LLSFLFILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLT 60
           MKNF  LLSFLFILLASTEVRF RADASP+AV DIDGKKLRAGVNYYILPVIRGRGGGLT
Sbjct: 1   MKNFGILLSFLFILLASTEVRFSRADASPEAVRDIDGKKLRAGVNYYILPVIRGRGGGLT 60

Query: 61  LGNLQSDKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120
           L NLQS+ CP+NVVQEQFE+MNG P TF PVNPKKGVVRVSTDLNVQFEASTICATSTVW
Sbjct: 61  LSNLQSENCPVNVVQEQFELMNGFPTTFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 121 KLDKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRDI 180
           KLDK DESTGQWFVTIGG++GNPGVETVDNWFKI KHG DYK VFCP+VCDFCKVMCRDI
Sbjct: 121 KLDKVDESTGQWFVTIGGSRGNPGVETVDNWFKIVKHGKDYKLVFCPSVCDFCKVMCRDI 180

Query: 181 GIFFKNGKRALALSDSPFPVMFKKV 205
           GIFFKNGKRALALSD+PFPVMFKKV
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKKV 205

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BLAST of Spg036426 vs. NCBI nr
Match: XP_008437058.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 381.3 bits (978), Expect = 5.1e-102
Identity = 178/203 (87.68%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 2   KNFALLSFLFILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTLG 61
           KNF +  F+FILLASTE+RF  ADASP+AVLDIDGKKLRAGVNYYILPV RGRGGGLTLG
Sbjct: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62

Query: 62  NLQSDKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 121
           NLQS+ CP+NVVQEQFE+MNG P TF PVNPKKGVVRVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 63  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 122

Query: 122 DKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRDIGI 181
           DKFDESTGQWFVTIGG++GNPGVETVDNWFKIEKHG DYK VFCPTVC+FCKVMCRDIGI
Sbjct: 123 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 182

Query: 182 FFKNGKRALALSDSPFPVMFKKV 205
           FFKNGKRALALSD+PFPVMFKKV
Sbjct: 183 FFKNGKRALALSDTPFPVMFKKV 205

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BLAST of Spg036426 vs. NCBI nr
Match: XP_004137534.1 (kunitz trypsin inhibitor 5 [Cucumis sativus])

HSP 1 Score: 375.6 bits (963), Expect = 2.8e-100
Identity = 180/206 (87.38%), Postives = 191/206 (92.72%), Query Frame = 0


Query: 1   MKNFALL-SFLFILLASTE-VRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGL 60
           MKNF +L  FLFILLAST+ +RF  ADASP+AVLDIDGKKLRAGVNYYILPV RGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSDKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTV 120
           TLGNLQS+KCPLNVVQEQ E+MNG P TF PVNPKKGVVRVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRD 180
           WKLDKFDESTGQW VTIGG++GNPGVETVDNWFKIEKHG DYK VFCPTVC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGKRALALSDSPFPVMFKKV 205
           IGIFFKNG+RALALSD+PFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

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BLAST of Spg036426 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 2.1e-58
Identity = 106/198 (53.54%), Postives = 145/198 (73.23%), Query Frame = 0


Query: 7   LSFLFILLA-STEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTLGNLQS 66
           L ++F+LLA     R    +A+ + V DI+GK L  GVNYYILPVIRGRGGGLT+ NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 67  DKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTVWKLDKFD 126
           + CP +V+Q+QFE+  GLP  F+P + K   + VSTD+N++F      + +++W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANFD 123

Query: 127 ESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRDIGIFFKN 186
           E+T QWF++  G +GNPG +TVDNWFKI+K   DYK  FCPTVC+FCKV+CRD+G+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 187 GKRALALSDSPFPVMFKK 204
           GKR LALSD P  VMFK+
Sbjct: 184 GKRRLALSDVPLKVMFKR 194

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BLAST of Spg036426 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 217.2 bits (552), Expect = 1.7e-55
Identity = 114/216 (52.78%), Postives = 141/216 (65.28%), Query Frame = 0


Query: 1   MKNFALLSFLF-----ILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRG 60
           MK   +LS  F     +L A+       AD++P+ VLDIDG+KLR G NYYI+PV+R  G
Sbjct: 1   MKELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHG 60

Query: 61  GGLTLGNLQSD---KCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTI 120
           GGLT+     +    CP  VVQ + E+ +  P  F P NPK+ VVRVSTDLN+ F A   
Sbjct: 61  GGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 120

Query: 121 C--ATSTVWKLDKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKH--GNDYKFVFCPTV 180
           C   +STVW+LDK+DESTGQ+FVTIGG KGNPG ET+ +WFKIE+      YK VFCPTV
Sbjct: 121 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTV 180

Query: 181 CDFCKVMCRDIGIFF-KNGKRALALSDSPFPVMFKK 204
           C  CKV C D+GI+  + G+R LALSD PF   F K
Sbjct: 181 CGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

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BLAST of Spg036426 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.1e-43
Identity = 96/208 (46.15%), Postives = 124/208 (59.62%), Query Frame = 0


Query: 1   MKNFALLSFLFILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGR-GGGLT 60
           MK    +  L     S    F  A+A+   VLD DG +L+ GV YY+L  I G  GGGL 
Sbjct: 1   MKTATAVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLA 60

Query: 61  LGNLQSDKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFE--ASTICATST 120
           LG      CP  VVQ + ++ NG P  F+  + K  VVRVSTD+N++F      +C+TST
Sbjct: 61  LGRATGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTST 120

Query: 121 VWKLDKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHG-NDYKFVFCPTVCDFCKVMC 180
           VW+LD +D S G+W+VT  G KG PG  T+ +WFKIEK G   YKF FCP+VCD C  +C
Sbjct: 121 VWRLDNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLC 180

Query: 181 RDIGIFF-KNGKRALALSDSPFPVMFKK 204
            DIG     +G+  LALSD+ +  MFKK
Sbjct: 181 SDIGRHSDDDGQIRLALSDNEWAWMFKK 208

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BLAST of Spg036426 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 4.6e-37
Identity = 88/196 (44.90%), Postives = 116/196 (59.18%), Query Frame = 0


Query: 11  FILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTLGNLQSDKCPL 70
           +++LA T V    A  +  AV+DIDG  +    +YY+LPVIRGRGGGLTL       CP 
Sbjct: 13  YLVLALTAV---LASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 71  NVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFE-ASTICATSTVWKLDKFDESTG 130
           ++VQE  E+  G+P  F+    K   V  S +LN++ +  +TIC  ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 131 QWFVTIGGTKGNPGVETVDNWFKIEKHGND-YKFVFCPTVCDFCKVMCRDIGIFFKN-GK 190
           Q+FV  G      G +++ ++FKIEK G D YKFVFCP  CD     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 191 RALALSDSPFPVMFKK 204
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of Spg036426 vs. ExPASy Swiss-Prot
Match: P80691 (Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 7.3e-27
Identity = 75/183 (40.98%), Postives = 106/183 (57.92%), Query Frame = 0


Query: 27  SPDAVLDIDGKKLRAGVNYYILPVIRGR-GGGLTL-GNLQSDKCPLNVVQEQFEIMNGLP 86
           +P  ++DIDGK +  GV+Y+++  I G  GGGLT+ G     KCPL+VVQ+ F+  NG P
Sbjct: 2   APKPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKKCPLSVVQDPFD--NGEP 61

Query: 87  ATFAPV-NPKKGVVRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGTKGNPG 146
             F+ + N K  +V  S DLNV+F  +  C  +T WK+D+F    G W VT+GG KG  G
Sbjct: 62  IIFSAIKNVKDNIVFESVDLNVKFNITINCNETTAWKVDRFPGVIG-WTVTLGGEKGYHG 121

Query: 147 VETVDNWFKIEKHG--NDYKFVFCPTVCDFCKVMCRDIGIFF-KNGKRALALSDSPFPVM 204
            E+  + FKI+K G    YKF FCP+      + C ++ IFF K   R L L++     +
Sbjct: 122 FESTHSMFKIKKAGLPFSYKFHFCPSYPRTRLIPCNNVDIFFDKYRIRRLILTNDAKEFV 181

[Close]

BLAST of Spg036426 vs. ExPASy TrEMBL
Match: A0A6J1KRM6 (kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.3e-105
Identity = 187/204 (91.67%), Postives = 194/204 (95.10%), Query Frame = 0


Query: 1   MKNFALLSFLFILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTL 60
           MKNFALLSFLFILLAS EVR  RADASPDAV DIDGKKLRAGVNYYILPV RGRGGGL L
Sbjct: 1   MKNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLAL 60

Query: 61  GNLQSDKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTVWK 120
           GNLQSDKCPLNVVQEQFE+MNGLPA F PVNPKKGVVRVSTDLNVQFEASTICATSTVWK
Sbjct: 61  GNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWK 120

Query: 121 LDKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRDIG 180
           LDKFDEST QWF+TIGGT+GNPGV+TVDNWFKIEKHGNDYKF FCPTVCDFCKVMCRD+G
Sbjct: 121 LDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVG 180

Query: 181 IFFKNGKRALALSDSPFPVMFKKV 205
           IFFKNGKRALALSD+PFPVMFK+V
Sbjct: 181 IFFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of Spg036426 vs. ExPASy TrEMBL
Match: A0A6J1EAD6 (kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.3e-105
Identity = 187/204 (91.67%), Postives = 194/204 (95.10%), Query Frame = 0


Query: 1   MKNFALLSFLFILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTL 60
           MKNFALLSFLFILLAS EVR  RADASPDAV DIDGKKLRAGVNYYILPV RGRGGGL L
Sbjct: 1   MKNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLAL 60

Query: 61  GNLQSDKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTVWK 120
           GNLQSDKCPLNVVQEQFE+MNGLPA F PVNPKKGVVRVSTDLNVQFEASTICATSTVWK
Sbjct: 61  GNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWK 120

Query: 121 LDKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRDIG 180
           LDKFDEST QWF+TIGGT+GNPGV+TVDNWFKIEKHGNDYKF FCPTVCDFCKVMCRD+G
Sbjct: 121 LDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVG 180

Query: 181 IFFKNGKRALALSDSPFPVMFKKV 205
           IFFKNGKRALALSD+PFPVMFK+V
Sbjct: 181 IFFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of Spg036426 vs. ExPASy TrEMBL
Match: A0A5D3BIG0 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002770 PE=3 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.5e-102
Identity = 178/203 (87.68%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 2   KNFALLSFLFILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTLG 61
           KNF +  F+FILLASTE+RF  ADASP+AVLDIDGKKLRAGVNYYILPV RGRGGGLTLG
Sbjct: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62

Query: 62  NLQSDKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 121
           NLQS+ CP+NVVQEQFE+MNG P TF PVNPKKGVVRVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 63  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 122

Query: 122 DKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRDIGI 181
           DKFDESTGQWFVTIGG++GNPGVETVDNWFKIEKHG DYK VFCPTVC+FCKVMCRDIGI
Sbjct: 123 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 182

Query: 182 FFKNGKRALALSDSPFPVMFKKV 205
           FFKNGKRALALSD+PFPVMFKKV
Sbjct: 183 FFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of Spg036426 vs. ExPASy TrEMBL
Match: A0A1S3ASR3 (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=3 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.5e-102
Identity = 178/203 (87.68%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 2   KNFALLSFLFILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTLG 61
           KNF +  F+FILLASTE+RF  ADASP+AVLDIDGKKLRAGVNYYILPV RGRGGGLTLG
Sbjct: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62

Query: 62  NLQSDKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 121
           NLQS+ CP+NVVQEQFE+MNG P TF PVNPKKGVVRVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 63  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 122

Query: 122 DKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRDIGI 181
           DKFDESTGQWFVTIGG++GNPGVETVDNWFKIEKHG DYK VFCPTVC+FCKVMCRDIGI
Sbjct: 123 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 182

Query: 182 FFKNGKRALALSDSPFPVMFKKV 205
           FFKNGKRALALSD+PFPVMFKKV
Sbjct: 183 FFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of Spg036426 vs. ExPASy TrEMBL
Match: A0A0A0LTW2 (Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.4e-100
Identity = 180/206 (87.38%), Postives = 191/206 (92.72%), Query Frame = 0


Query: 1   MKNFALL-SFLFILLASTE-VRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGL 60
           MKNF +L  FLFILLAST+ +RF  ADASP+AVLDIDGKKLRAGVNYYILPV RGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  TLGNLQSDKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTV 120
           TLGNLQS+KCPLNVVQEQ E+MNG P TF PVNPKKGVVRVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRD 180
           WKLDKFDESTGQW VTIGG++GNPGVETVDNWFKIEKHG DYK VFCPTVC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 IGIFFKNGKRALALSDSPFPVMFKKV 205
           IGIFFKNG+RALALSD+PFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

[Close]

BLAST of Spg036426 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 226.9 bits (577), Expect = 1.5e-59
Identity = 106/198 (53.54%), Postives = 145/198 (73.23%), Query Frame = 0


Query: 7   LSFLFILLA-STEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTLGNLQS 66
           L ++F+LLA     R    +A+ + V DI+GK L  GVNYYILPVIRGRGGGLT+ NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 67  DKCPLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEASTICATSTVWKLDKFD 126
           + CP +V+Q+QFE+  GLP  F+P + K   + VSTD+N++F      + +++W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANFD 123

Query: 127 ESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKVMCRDIGIFFKN 186
           E+T QWF++  G +GNPG +TVDNWFKI+K   DYK  FCPTVC+FCKV+CRD+G+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 187 GKRALALSDSPFPVMFKK 204
           GKR LALSD P  VMFK+
Sbjct: 184 GKRRLALSDVPLKVMFKR 194

[Close]

BLAST of Spg036426 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 156.0 bits (393), Expect = 3.3e-38
Identity = 88/196 (44.90%), Postives = 116/196 (59.18%), Query Frame = 0


Query: 11  FILLASTEVRFCRADASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTLGNLQSDKCPL 70
           +++LA T V    A  +  AV+DIDG  +    +YY+LPVIRGRGGGLTL       CP 
Sbjct: 13  YLVLALTAV---LASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 71  NVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFE-ASTICATSTVWKLDKFDESTG 130
           ++VQE  E+  G+P  F+    K   V  S +LN++ +  +TIC  ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 131 QWFVTIGGTKGNPGVETVDNWFKIEKHGND-YKFVFCPTVCDFCKVMCRDIGIFFKN-GK 190
           Q+FV  G      G +++ ++FKIEK G D YKFVFCP  CD     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 191 RALALSDSPFPVMFKK 204
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of Spg036426 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.7e-23
Identity = 73/211 (34.60%), Postives = 113/211 (53.55%), Query Frame = 0


Query: 1   MKNFALLSFLFILLASTEVRFCRADASP-DAVLDIDGKKLRAGVNYYILPVIRGRGGGLT 60
           M+   L      +L++       ADA+P   VLDI G  +++ V YYI+P   G GGGL 
Sbjct: 1   MEKLTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLI 60

Query: 61  LGNLQ---SDKC-PLNVVQEQFEIMNGLPATFAPVNPKKGVVRVSTDLNVQFEAST-ICA 120
             N      D C  L++VQ     ++GLP TF+P+N K   V++S  LN++F+++  +C 
Sbjct: 61  PSNRNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCP 120

Query: 121 TSTVWKLDKFDESTGQWFVTIGGTKGNPGVETVDNWFKIEKHGNDYKFVFCPTVCDFCKV 180
            S VW++D       + FV+IGG KG       ++WF+I++ G+ YK ++CP       V
Sbjct: 121 DSKVWRID-HSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIV 180

Query: 181 MCRDIGI-FFKNGKRALALS-DSPFPVMFKK 204
            C ++ +    +G R L LS D  F V F+K
Sbjct: 181 ACINVSLEIDDHGVRRLVLSTDQSFVVKFQK 202

The following BLAST results are available for this feature:

BLAST of Spg036426 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022923703.1	1.1e-104	91.67	kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin i...	[more]
XP_023520252.1	9.3e-104	91.18	kunitz trypsin inhibitor 2 [Cucurbita pepo subsp. pepo]	[more]
XP_038895203.1	1.0e-102	89.76	kunitz trypsin inhibitor 5-like [Benincasa hispida]	[more]
XP_008437058.1	5.1e-102	87.68	PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis mel...	[more]
XP_004137534.1	2.8e-100	87.38	kunitz trypsin inhibitor 5 [Cucumis sativus]	[more]

BLAST of Spg036426 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMU2	2.1e-58	53.54	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P13087	1.7e-55	52.78	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P32765	1.1e-43	46.15	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	4.6e-37	44.90	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P80691	7.3e-27	40.98	Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1	[more]

BLAST of Spg036426 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KRM6	5.3e-105	91.67	kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1	[more]
A0A6J1EAD6	5.3e-105	91.67	kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV...	[more]
A0A5D3BIG0	2.5e-102	87.68	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00277...	[more]
A0A1S3ASR3	2.5e-102	87.68	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=3 SV=1	[more]
A0A0A0LTW2	1.4e-100	87.38	Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=3 SV=1	[more]

BLAST of Spg036426 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	1.5e-59	53.54	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	3.3e-38	44.90	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	1.7e-23	34.60	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 150..169 score: 25.49 coord: 68..88 score: 31.93 coord: 173..202 score: 25.16 coord: 30..59 score: 47.25
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 30..204 e-value: 3.0E-75 score: 266.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 31..203 e-value: 1.0E-60 score: 204.6
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..204
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 31..47
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 30..203 e-value: 5.74839E-60 score: 183.698
None	No IPR available	GENE3D	2.80.10.50		coord: 25..204 e-value: 3.3E-77 score: 260.1
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..204
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 27..204

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg036426.1	Spg036426.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity