Spg036365 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg036365
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Sphingolipid delta(4)-desaturase DES1-like
Location	scaffold5: 41755994 .. 41758609 (-)
RNA-Seq Expression	Spg036365
Synteny	Spg036365

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATTTCTACGTGACGAGCCCCCAGGAGAGGCAGACTCCGCCATAGCATCTTCTGGGAGCGAGGGCCACGCGCAAGCACAGACAATGGCAGCAGCTGAAGACAGAGACGAGGGCGTAATGGCCACCGACTTCTTCTGGTCATACACCGACGAGCCTCACGCTTCCAGACGACGCCAAATCCTCTCTCGGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGGTCTCGTTTCTTCTCCCTGATTTTTCTCTTTTGTGTGTTGCTGATTCTGAGAATTTGGACAGTTGTGGTTATTTTCTTGTCAGGGGATGTTTTGATTTCGTGTCTAGATCTGAATTGGGTTATTGCTGGTCGCACCACATGTCGATGGCTGAATCTGATGATTCTGATCTGTGTATATTTGTTTTTCTGTTTGATTTGAATCTTACCTCTGCCACATTGCTGCATTCGACATTGCTTTTGTTTTCCCGTCCCGATTACTCTTGCTAGTTGTATTGATCGAAAATCTAGATTTGAGATTCTGAACGCTTGTTATTGAGGGATCTGGCCGCTTCGCTGCGTCTGAAATTTTGTCATTCTTATTGCTTAGGTTCAGTTTTTGCGCTATGTTCCTGTTTTCGATCTTTTTGTTTCTTTTTTCTTACTTGAATCTTGAGCATCTGTCTGAAGTTTCCTCTGCTTTCACAACCATCAGACAAGCTTTTATTCAGGATTGTTTTGGTCTAAAAGTTATTTTAAGTTACCCTTCTTTTCTTATTTAGATTTCAAAAGAATGCCGTGGAAGAAATAGAACTTTACCCCTTTTTTTATTACGACTTGGAAAAGTATTGCGATCCTAAAGAATTGTGTTGTTTGCTCAATGATATCATGCCTTCTTCAATCGGCCATTTTCTCCAAGTTTTTGGTAAATGTGGAAGTTTAAATGGCTATAATTCATTCTACTATGCACTATCGAGGTTCTGTAGAGAAAATACGTCGCTTTTTTGGCTCAAATATCTGATTTTAGCTGACGTTTAATTGTTTGATTAGGGTGGTTCATTGCTTTGAGGGGACTGTATAGTTTCATGTGAAACCATTCATGAAATCAGAAAAAAAATGACCCTTGAATTTAATTTTTAGACTATTGCTTCCTGGTGTTGAATTGGGGGGGACTGATTTTGTTGGCTGTATTTGTCAAGAAGAGAAGATTGATTGTAATTATTAAACAAACTGCATCAGCATTCTGATTCTTCTGATTAAGAAAGTAAAGTCATGAAAAATTAGAAATTGGATTGAACAACGATTTATGTATTCACAATCACTAGTGACCTGACCTCCTGTAGTCTTAAAGTTGAAATTGGGAAACCAACCATCAACTTGTTCTTGTAATATTAATTGGGGCCATTTTTTCTTCTCATTCTTGCAATCTTGCAGATTACTGTGGTTGTTCTGCTTCAGTTATGGACTGCTACGACCCTTCACAGTGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTGTTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGATCACAACCGGTGGCTTGGGATTTTTGCGAATCTCCCCATTGGAGTACCCATGTCCATCACATTTCAAAAGTATCACCTGGAACACCATCGCTTCCAAGGAGTGGATGGCGTTGATATGGACATTCCGAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTTTCAAAAGCCATATGGGTTGTCTTCCAACTCTTCTTCTATGCTCTTCGTCCATTGTTTCTCAAACCAAAGCCCCCAGGCTCCTGGGAGTTCATCAACTTCATCATTCAGATAGCTCTTGATTTCACCATGGTTTACTTTTGGAGCTGGAAGTCTTTTGCTTATCTGATCCTCTCCACATTCGTCGGTGGCGGGATGCATCCGATGGCTGGTCACTTCATTTCCGAACATTACGTCTTCAATCCCAAACAGGAGACCTACTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTGGGTTACCACAATGAACACCATGATTTCCCCAGGATTCCAGGAAGCAAGCTTTACAAGGTGAAGGAGATTGCACCTGAATTCTACAACGAATTAGATTCATATAAATCATGGAGCCAGGTGATATACATGTACATTATGGACAGGACAGTGGGACCATTCAGTCGGATGAAGAGAATGGGAAAGAAATCCGAGTAGTTGATCCTTTTTCTCTCCCTCTTAGATTCTTTTTGTTCAATTCTTTTTCCATCTTTTTTGATGGATCATTCTGTCTTGAGCTGGTTTTGCCATTGTTTTGTTTACGTTCTTGGAGAGTTTACTTATTCTTATTTAGATTGTATAGTAGCCAGGCATGTTCATTGTTCACTGTACTGTATGTAATCGATTTTCAGGGCTTTTTGAGGGACCGTTTGTAAAAGGAGCCGAGCGTTAGCGAAGCTAGATTCTCATAGGTTTGGTATTTGGTTAATTTTCAATTGTAGTTCCTCTATACTTTTGAGGACTTTGCCTATATATATATTCATCTTAATGTGTCCGAACTTTGGACTTTAAATTGTGTTTACC

mRNA sequence

AAATTTCTACGTGACGAGCCCCCAGGAGAGGCAGACTCCGCCATAGCATCTTCTGGGAGCGAGGGCCACGCGCAAGCACAGACAATGGCAGCAGCTGAAGACAGAGACGAGGGCGTAATGGCCACCGACTTCTTCTGGTCATACACCGACGAGCCTCACGCTTCCAGACGACGCCAAATCCTCTCTCGGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGGTCTCGTTTCTTCTCCCTGATTTTTCTCTTTTGTGTGTTGCTGATTCTGAGAATTTGGACAGTTGTGGTTATTTTCTTGTCAGGGGATGTTTTGATTTCGTGTCTAGATCTGAATTGGGTTATTGCTGGTCGCACCACATGTCGATGGCTGAATCTGATGATTCTGATCTGTGTATATTTGTTTTTCTGTTTGATTTGAATCTTACCTCTGCCACATTGCTGCATTCGACATTGCTTTTGGTGGTTCATTGCTTTGAGGGGACTATTACTGTGGTTGTTCTGCTTCAGTTATGGACTGCTACGACCCTTCACAGTGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTGTTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGATCACAACCGGTGGCTTGGGATTTTTGCGAATCTCCCCATTGGAGTACCCATGTCCATCACATTTCAAAAGTATCACCTGGAACACCATCGCTTCCAAGGAGTGGATGGCGTTGATATGGACATTCCGAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTTTCAAAAGCCATATGGGTTGTCTTCCAACTCTTCTTCTATGCTCTTCGTCCATTGTTTCTCAAACCAAAGCCCCCAGGCTCCTGGGAGTTCATCAACTTCATCATTCAGATAGCTCTTGATTTCACCATGGTTTACTTTTGGAGCTGGAAGTCTTTTGCTTATCTGATCCTCTCCACATTCGTCGGTGGCGGGATGCATCCGATGGCTGGTCACTTCATTTCCGAACATTACGTCTTCAATCCCAAACAGGAGACCTACTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTGGGTTACCACAATGAACACCATGATTTCCCCAGGATTCCAGGAAGCAAGCTTTACAAGGTGAAGGAGATTGCACCTGAATTCTACAACGAATTAGATTCATATAAATCATGGAGCCAGGTGATATACATGTACATTATGGACAGGACAGTGGGACCATTCAGTCGGATGAAGAGAATGGGAAAGAAATCCGAGTAG

Coding sequence (CDS)

AAATTTCTACGTGACGAGCCCCCAGGAGAGGCAGACTCCGCCATAGCATCTTCTGGGAGCGAGGGCCACGCGCAAGCACAGACAATGGCAGCAGCTGAAGACAGAGACGAGGGCGTAATGGCCACCGACTTCTTCTGGTCATACACCGACGAGCCTCACGCTTCCAGACGACGCCAAATCCTCTCTCGGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGGTCTCGTTTCTTCTCCCTGATTTTTCTCTTTTGTGTGTTGCTGATTCTGAGAATTTGGACAGTTGTGGTTATTTTCTTGTCAGGGGATGTTTTGATTTCGTGTCTAGATCTGAATTGGGTTATTGCTGGTCGCACCACATGTCGATGGCTGAATCTGATGATTCTGATCTGTGTATATTTGTTTTTCTGTTTGATTTGAATCTTACCTCTGCCACATTGCTGCATTCGACATTGCTTTTGGTGGTTCATTGCTTTGAGGGGACTATTACTGTGGTTGTTCTGCTTCAGTTATGGACTGCTACGACCCTTCACAGTGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTGTTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGATCACAACCGGTGGCTTGGGATTTTTGCGAATCTCCCCATTGGAGTACCCATGTCCATCACATTTCAAAAGTATCACCTGGAACACCATCGCTTCCAAGGAGTGGATGGCGTTGATATGGACATTCCGAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTTTCAAAAGCCATATGGGTTGTCTTCCAACTCTTCTTCTATGCTCTTCGTCCATTGTTTCTCAAACCAAAGCCCCCAGGCTCCTGGGAGTTCATCAACTTCATCATTCAGATAGCTCTTGATTTCACCATGGTTTACTTTTGGAGCTGGAAGTCTTTTGCTTATCTGATCCTCTCCACATTCGTCGGTGGCGGGATGCATCCGATGGCTGGTCACTTCATTTCCGAACATTACGTCTTCAATCCCAAACAGGAGACCTACTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTGGGTTACCACAATGAACACCATGATTTCCCCAGGATTCCAGGAAGCAAGCTTTACAAGGTGAAGGAGATTGCACCTGAATTCTACAACGAATTAGATTCATATAAATCATGGAGCCAGGTGATATACATGTACATTATGGACAGGACAGTGGGACCATTCAGTCGGATGAAGAGAATGGGAAAGAAATCCGAGTAG

Protein sequence

KFLRDEPPGEADSAIASSGSEGHAQAQTMAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDFSLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNLTSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQTEAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE

Homology

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BLAST of Spg036365 vs. NCBI nr
Match: KAG7012427.1 (Sphingolipid delta(4)-desaturase DES1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 618.6 bits (1594), Expect = 4.1e-173
Identity = 308/412 (74.76%), Postives = 315/412 (76.46%), Query Frame = 0


Query: 29  MAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDF 88
           M   E+RDEGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFK        
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFK-------- 60

Query: 89  SLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNL 148
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 149 TSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 208
                               I VVVLLQLWTATTLHSADWLKILA+AYFFGSFLNHNLFL
Sbjct: 121 --------------------IAVVVLLQLWTATTLHSADWLKILAVAYFFGSFLNHNLFL 180

Query: 209 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQT 268
           AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDG+DMD+PS T
Sbjct: 181 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSMT 240

Query: 269 EAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKS 328
           EA LVTN+VSKAIWVVFQLFFYALRPLFLKPKPPG WEFINFIIQI LDFTMVYFWSWK+
Sbjct: 241 EARLVTNIVSKAIWVVFQLFFYALRPLFLKPKPPGHWEFINFIIQIGLDFTMVYFWSWKT 300

Query: 329 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 388
           FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI
Sbjct: 301 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 324

Query: 389 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRM KKSE
Sbjct: 361 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMAKKSE 324

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BLAST of Spg036365 vs. NCBI nr
Match: XP_022955306.1 (sphingolipid delta(4)-desaturase DES1-like [Cucurbita moschata] >KAG6573258.1 Sphingolipid delta(4)-desaturase DES1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 618.2 bits (1593), Expect = 5.4e-173
Identity = 308/412 (74.76%), Postives = 315/412 (76.46%), Query Frame = 0


Query: 29  MAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDF 88
           M   E+RDEGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFK        
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFK-------- 60

Query: 89  SLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNL 148
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 149 TSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 208
                               I VVVLLQLWTATTLHSADWLKILA+AYFFGSFLNHNLFL
Sbjct: 121 --------------------IAVVVLLQLWTATTLHSADWLKILAVAYFFGSFLNHNLFL 180

Query: 209 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQT 268
           AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDG+DMD+PS T
Sbjct: 181 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSTT 240

Query: 269 EAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKS 328
           EA LVTN+VSKAIWVVFQLFFYALRPLFLKPKPPG WEFINFIIQI LDFTMVYFWSWK+
Sbjct: 241 EARLVTNIVSKAIWVVFQLFFYALRPLFLKPKPPGHWEFINFIIQIGLDFTMVYFWSWKT 300

Query: 329 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 388
           FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI
Sbjct: 301 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 324

Query: 389 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRM KKSE
Sbjct: 361 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMAKKSE 324

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BLAST of Spg036365 vs. NCBI nr
Match: XP_023542715.1 (sphingolipid delta(4)-desaturase DES1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 615.9 bits (1587), Expect = 2.7e-172
Identity = 307/412 (74.51%), Postives = 314/412 (76.21%), Query Frame = 0


Query: 29  MAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDF 88
           M   E+ DEGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFK        
Sbjct: 1   MPRGEETDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFK-------- 60

Query: 89  SLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNL 148
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 149 TSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 208
                               I VVVLLQLWTATTLHSADWLKILA+AYFFGSFLNHNLFL
Sbjct: 121 --------------------IAVVVLLQLWTATTLHSADWLKILAVAYFFGSFLNHNLFL 180

Query: 209 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQT 268
           AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDG+DMD+PS T
Sbjct: 181 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSTT 240

Query: 269 EAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKS 328
           EA LVTN+VSKAIWVVFQLFFYALRPLFLKPKPPG WEFINFIIQI LDFTMVYFWSWK+
Sbjct: 241 EARLVTNIVSKAIWVVFQLFFYALRPLFLKPKPPGHWEFINFIIQIGLDFTMVYFWSWKT 300

Query: 329 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 388
           FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI
Sbjct: 301 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 324

Query: 389 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRM KKSE
Sbjct: 361 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMAKKSE 324

[Close]

BLAST of Spg036365 vs. NCBI nr
Match: XP_004146595.1 (sphingolipid delta(4)-desaturase DES1-like [Cucumis sativus] >KGN64668.1 hypothetical protein Csa_014335 [Cucumis sativus])

HSP 1 Score: 615.5 bits (1586), Expect = 3.5e-172
Identity = 305/412 (74.03%), Postives = 317/412 (76.94%), Query Frame = 0


Query: 29  MAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDF 88
           M   E+RD+GVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFK+S      
Sbjct: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIS------ 60

Query: 89  SLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNL 148
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 149 TSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 208
                                 VVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL
Sbjct: 121 ----------------------VVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 180

Query: 209 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQT 268
           AIHELSHNLAFSTPD+N+WLGIFANLPIGVPMSITFQKYHLEHHRFQGVDG+DMD+PSQT
Sbjct: 181 AIHELSHNLAFSTPDYNKWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSQT 240

Query: 269 EAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKS 328
           EAHLVTNVVSKAIWV+FQLFFYALRPLFLKPKPPG WEFINFIIQIALD  MVYFWSWKS
Sbjct: 241 EAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGHWEFINFIIQIALDVAMVYFWSWKS 300

Query: 329 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 388
           FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI
Sbjct: 301 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 324

Query: 389 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           PGSKLYKVKEIAPE+YNELDSYKSWSQVIYMYIMD+TVGPFSRMKRM +KSE
Sbjct: 361 PGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDKTVGPFSRMKRMPRKSE 324

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BLAST of Spg036365 vs. NCBI nr
Match: XP_038893930.1 (sphingolipid delta(4)-desaturase DES1-like [Benincasa hispida])

HSP 1 Score: 614.8 bits (1584), Expect = 5.9e-172
Identity = 307/412 (74.51%), Postives = 315/412 (76.46%), Query Frame = 0


Query: 29  MAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDF 88
           M   E+RDEGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFK+S      
Sbjct: 1   MQGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIS------ 60

Query: 89  SLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNL 148
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 149 TSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 208
                                 VVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL
Sbjct: 121 ----------------------VVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 180

Query: 209 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQT 268
           AIHELSHNLAFSTPD+NRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDG+DMD+PS T
Sbjct: 181 AIHELSHNLAFSTPDYNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSLT 240

Query: 269 EAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKS 328
           EAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPG WEFINFIIQIALD  MVY WSWKS
Sbjct: 241 EAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGQWEFINFIIQIALDVAMVYLWSWKS 300

Query: 329 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 388
           FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI
Sbjct: 301 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 324

Query: 389 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           PGSKLYKVKEIAPE+YNELDSYKSWSQVIYMYIMDRTVGPFSRMKRM +KSE
Sbjct: 361 PGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMPRKSE 324

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BLAST of Spg036365 vs. ExPASy Swiss-Prot
Match: Q6H5U3 (Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0639600 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 4.6e-151
Identity = 268/413 (64.89%), Postives = 292/413 (70.70%), Query Frame = 0


Query: 30  AAAED--RDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPD 89
           AAA D   +EGVMATDFFWSYTDEPHA+RRR+IL+++PQI+ELFGPDP AF K       
Sbjct: 3   AAAGDGREEEGVMATDFFWSYTDEPHATRRREILAKHPQIKELFGPDPLAFLK------- 62

Query: 90  FSLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLN 149
                                                                       
Sbjct: 63  ------------------------------------------------------------ 122

Query: 150 LTSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLF 209
                                I  VV LQLWTAT L  A W+KIL +AYFFGSFLNHNLF
Sbjct: 123 ---------------------IAAVVSLQLWTATLLRDASWVKILTVAYFFGSFLNHNLF 182

Query: 210 LAIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQ 269
           LAIHELSHNLAF+TP +NRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDG+DMDIPSQ
Sbjct: 183 LAIHELSHNLAFTTPSYNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGIDMDIPSQ 242

Query: 270 TEAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWK 329
            EAH V N +SK++WVVFQLFFYALRPLFLKPKPPG WEF N IIQIALD +MVYF+ WK
Sbjct: 243 AEAHAVKNTLSKSVWVVFQLFFYALRPLFLKPKPPGLWEFTNLIIQIALDASMVYFFGWK 302

Query: 330 SFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPR 389
           S AYLILSTFVGGGMHPMAGHFISEHYVFNP QETYSYYGPLNL+TW VGYHNEHHDFPR
Sbjct: 303 SLAYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLMTWHVGYHNEHHDFPR 327

Query: 390 IPGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           IPG++LYKV+EIAPE+YN L SYKSWSQVIYMYIMD+TVGPFSRMKR   K +
Sbjct: 363 IPGTRLYKVREIAPEYYNNLKSYKSWSQVIYMYIMDQTVGPFSRMKRKAPKKD 327

[Close]

BLAST of Spg036365 vs. ExPASy Swiss-Prot
Match: Q9ZPH4 (Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana OX=3702 GN=At4g04930 PE=2 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 5.6e-149
Identity = 265/419 (63.25%), Postives = 296/419 (70.64%), Query Frame = 0


Query: 22  GHAQAQTMAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKV 81
           G    + +++ E+  EGVMATDFFWSYTDEPHASRRRQILS YPQIR+LFGPDPWAF K 
Sbjct: 2   GKGGREKISSNEEEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLK- 61

Query: 82  SFLLPDFSLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFV 141
                                                                       
Sbjct: 62  ------------------------------------------------------------ 121

Query: 142 FLFDLNLTSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSF 201
                                      IT+VV+LQL TA  LH++ WLKIL+IAYFFGSF
Sbjct: 122 ---------------------------ITLVVILQLSTAAILHNSGWLKILSIAYFFGSF 181

Query: 202 LNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVD 261
           LNHNLFLAIHELSHNLAFSTP +NR LGIFANLPIGVPMS+TFQKYHLEHHRFQGVDG+D
Sbjct: 182 LNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPIGVPMSVTFQKYHLEHHRFQGVDGID 241

Query: 262 MDIPSQTEAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMV 321
           MD+P+ TEAHLVTN+ +K IWV  QLFFYALRP+F+KPKPPG WEFINF+IQI LD ++V
Sbjct: 242 MDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIFIKPKPPGYWEFINFLIQIVLDVSVV 301

Query: 322 YFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNE 381
            F+ W+SFAYLILSTFVGGGMHPMAGHFISEHYVFNP QETYSYYGPLNLLTWSVGYHNE
Sbjct: 302 LFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQETYSYYGPLNLLTWSVGYHNE 332

Query: 382 HHDFPRIPGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           HHDFPRIPG+KL+ VKEIA E+Y  L+SYKSWSQVIYMYIMD TVGP+SRMKR   KS+
Sbjct: 362 HHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQVIYMYIMDTTVGPYSRMKRKLSKSD 332

[Close]

BLAST of Spg036365 vs. ExPASy Swiss-Prot
Match: Q6UQ04 (Sphingolipid delta(4)-desaturase DES1 OS=Microtus fortis OX=100897 GN=DEGS1 PE=2 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 3.1e-83
Identity = 161/404 (39.85%), Postives = 218/404 (53.96%), Query Frame = 0


Query: 39  VMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDFSLLCVADSEN 98
           V   +F W YTD+PH  RRR+IL++YP+I+ L  PDP                       
Sbjct: 5   VSREEFEWVYTDQPHTDRRREILAKYPEIKSLMKPDP----------------------- 64

Query: 99  LDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNLTSATLLHSTL 158
                                   W                                   
Sbjct: 65  ---------------------NLIW----------------------------------- 124

Query: 159 LLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLA 218
                     IT + L+QL +   +   DW  ++  +Y FGS LNH++ LAIHE+SHN  
Sbjct: 125 ---------IITAMFLVQLISLYLVKDLDWKWVIFWSYVFGSCLNHSMTLAIHEISHNFP 184

Query: 219 FS--TPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQTEAHLVTNV 278
           F       NRW GIFANLPIGVP S++F++YH++HHR+ G DG+D+DIP+  E       
Sbjct: 185 FGHHKAVWNRWFGIFANLPIGVPYSVSFKRYHMDHHRYLGADGIDVDIPTDFEGWFFCTT 244

Query: 279 VSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKSFAYLILST 338
             K +WV+ Q  FYALRPLF+ PKP    E IN + QI LD  + Y +  KS  Y++ ++
Sbjct: 245 FRKLVWVILQPLFYALRPLFINPKPITYLEIINTVTQIILDIIVYYVFGIKSLVYMLAAS 304

Query: 339 FVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKV 398
            +G G+HP++GHFI+EHY+F    ETYSYYGPLNLLT++VGYHNEHHDFP +PG  L  V
Sbjct: 305 LLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNVPGKNLPLV 320

Query: 399 KEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           ++IA E+Y++L  Y SW +V+Y ++MD T+ P+SRMKR  K SE
Sbjct: 365 RKIAAEYYDKLPQYNSWIKVLYDFVMDDTISPYSRMKRPPKGSE 320

[Close]

BLAST of Spg036365 vs. ExPASy Swiss-Prot
Match: O15121 (Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens OX=9606 GN=DEGS1 PE=1 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 3.5e-82
Identity = 161/401 (40.15%), Postives = 214/401 (53.37%), Query Frame = 0


Query: 39  VMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDFSLLCVADSEN 98
           V   DF W YTD+PHA RRR+IL++YP+I+ L  PDP                       
Sbjct: 5   VSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDP----------------------- 64

Query: 99  LDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNLTSATLLHSTL 158
                                   W                                   
Sbjct: 65  ---------------------NLIW----------------------------------- 124

Query: 159 LLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLA 218
                     I ++VL QL     +   DW  ++  AY FGS +NH++ LAIHE++HN A
Sbjct: 125 ---------IIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAA 184

Query: 219 FST--PDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQTEAHLVTNV 278
           F       NRW G+FANLPIG+P SI+F++YH++HHR+ G DGVD+DIP+  E       
Sbjct: 185 FGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTA 244

Query: 279 VSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKSFAYLILST 338
             K IWV+ Q  FYA RPLF+ PKP    E IN + Q+  D  + YF   KS  Y++ ++
Sbjct: 245 FRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAAS 304

Query: 339 FVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKV 398
            +G G+HP++GHFI+EHY+F    ETYSYYGPLNLLT++VGYHNEHHDFP IPG  L  V
Sbjct: 305 LLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLV 317

Query: 399 KEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGK 438
           ++IA E+Y+ L  Y SW +V+Y ++MD T+ P+SRMKR  K
Sbjct: 365 RKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQK 317

[Close]

BLAST of Spg036365 vs. ExPASy Swiss-Prot
Match: Q5RE51 (Sphingolipid delta(4)-desaturase DES1 OS=Pongo abelii OX=9601 GN=DEGS1 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.9e-81
Identity = 159/401 (39.65%), Postives = 212/401 (52.87%), Query Frame = 0


Query: 39  VMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDFSLLCVADSEN 98
           V   DF W YTD+PHA RRR+IL++YP+I+ L  PDP                       
Sbjct: 5   VSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDP----------------------- 64

Query: 99  LDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNLTSATLLHSTL 158
                                   W                                   
Sbjct: 65  ---------------------NLIW----------------------------------- 124

Query: 159 LLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLA 218
                     I ++VL QL     +   DW  ++  AY FGS +NH++ L IHE++HN A
Sbjct: 125 ---------IIIMMVLTQLAAFYIVKDLDWKWVIFGAYAFGSCINHSMTLGIHEIAHNAA 184

Query: 219 FST--PDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQTEAHLVTNV 278
           F       NRW G+FANLPIG+P S++F+ YH++HHR+ G DGVD+DIP+  E       
Sbjct: 185 FGNCKAMWNRWFGMFANLPIGIPYSVSFKSYHMDHHRYLGADGVDVDIPTDFEGWFFCTA 244

Query: 279 VSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKSFAYLILST 338
             K IWV+ Q  FYA RPLF+ PKP    E IN + Q+  D  + YF   KS  Y++ ++
Sbjct: 245 FRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAAS 304

Query: 339 FVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKV 398
            +G G+HP++GHFI+EHY+F    ETYSYYGPLNLLT++VGYHNEHHDFP IPG  L  V
Sbjct: 305 LLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLV 317

Query: 399 KEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGK 438
           ++IA E+Y+ L  Y SW +V+Y ++MD T+ P+SRMKR  K
Sbjct: 365 RKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQK 317

[Close]

BLAST of Spg036365 vs. ExPASy TrEMBL
Match: A0A6J1GTF2 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC111457308 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.6e-173
Identity = 308/412 (74.76%), Postives = 315/412 (76.46%), Query Frame = 0


Query: 29  MAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDF 88
           M   E+RDEGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFK        
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFK-------- 60

Query: 89  SLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNL 148
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 149 TSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 208
                               I VVVLLQLWTATTLHSADWLKILA+AYFFGSFLNHNLFL
Sbjct: 121 --------------------IAVVVLLQLWTATTLHSADWLKILAVAYFFGSFLNHNLFL 180

Query: 209 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQT 268
           AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDG+DMD+PS T
Sbjct: 181 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSTT 240

Query: 269 EAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKS 328
           EA LVTN+VSKAIWVVFQLFFYALRPLFLKPKPPG WEFINFIIQI LDFTMVYFWSWK+
Sbjct: 241 EARLVTNIVSKAIWVVFQLFFYALRPLFLKPKPPGHWEFINFIIQIGLDFTMVYFWSWKT 300

Query: 329 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 388
           FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI
Sbjct: 301 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 324

Query: 389 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRM KKSE
Sbjct: 361 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMAKKSE 324

[Close]

BLAST of Spg036365 vs. ExPASy TrEMBL
Match: A0A0A0LSB9 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis sativus OX=3659 GN=Csa_1G073750 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.7e-172
Identity = 305/412 (74.03%), Postives = 317/412 (76.94%), Query Frame = 0


Query: 29  MAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDF 88
           M   E+RD+GVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFK+S      
Sbjct: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIS------ 60

Query: 89  SLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNL 148
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 149 TSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 208
                                 VVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL
Sbjct: 121 ----------------------VVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 180

Query: 209 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQT 268
           AIHELSHNLAFSTPD+N+WLGIFANLPIGVPMSITFQKYHLEHHRFQGVDG+DMD+PSQT
Sbjct: 181 AIHELSHNLAFSTPDYNKWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSQT 240

Query: 269 EAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKS 328
           EAHLVTNVVSKAIWV+FQLFFYALRPLFLKPKPPG WEFINFIIQIALD  MVYFWSWKS
Sbjct: 241 EAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGHWEFINFIIQIALDVAMVYFWSWKS 300

Query: 329 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 388
           FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI
Sbjct: 301 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 324

Query: 389 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           PGSKLYKVKEIAPE+YNELDSYKSWSQVIYMYIMD+TVGPFSRMKRM +KSE
Sbjct: 361 PGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]

BLAST of Spg036365 vs. ExPASy TrEMBL
Match: A0A6J1K2T6 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita maxima OX=3661 GN=LOC111490149 PE=3 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 4.9e-172
Identity = 306/412 (74.27%), Postives = 313/412 (75.97%), Query Frame = 0


Query: 29  MAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDF 88
           M   E+RDEGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFK        
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFK-------- 60

Query: 89  SLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNL 148
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 149 TSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 208
                               I VVVLLQLWTATTLHSADWLKILA+AYFFGSFLNHNLFL
Sbjct: 121 --------------------IAVVVLLQLWTATTLHSADWLKILAVAYFFGSFLNHNLFL 180

Query: 209 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQT 268
           AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDG+DMD+PS T
Sbjct: 181 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSMT 240

Query: 269 EAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKS 328
           EA LVTN+V KAIWVVFQLFFYALRPLFLKPKPPG WEFINFIIQI LDFTMVY WSWK+
Sbjct: 241 EARLVTNIVLKAIWVVFQLFFYALRPLFLKPKPPGHWEFINFIIQIGLDFTMVYLWSWKT 300

Query: 329 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 388
           FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI
Sbjct: 301 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 324

Query: 389 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRM KKSE
Sbjct: 361 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMAKKSE 324

[Close]

BLAST of Spg036365 vs. ExPASy TrEMBL
Match: A0A5D3DSS5 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00680 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.4e-171
Identity = 303/412 (73.54%), Postives = 316/412 (76.70%), Query Frame = 0


Query: 29  MAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDF 88
           M    +RD+GVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFK+S      
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKIS------ 60

Query: 89  SLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNL 148
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 149 TSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 208
                                 +VVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL
Sbjct: 121 ----------------------MVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 180

Query: 209 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQT 268
           AIHELSHNLAFSTPD+N+WLGIFANLPIGVPMSITFQKYHLEHHRFQGVDG+DMD+PSQT
Sbjct: 181 AIHELSHNLAFSTPDYNKWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSQT 240

Query: 269 EAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKS 328
           EAHLVTNVVSKAIWV+FQLFFYALRPLFLKPKPPG WEFINFIIQIALD  MVYFWSWKS
Sbjct: 241 EAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGHWEFINFIIQIALDVAMVYFWSWKS 300

Query: 329 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 388
           FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI
Sbjct: 301 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 324

Query: 389 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           PGSKLYKVKEIAPE+YNELDSYKSWSQVIYMYIMD+TVGPFSRMKRM +KSE
Sbjct: 361 PGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]

BLAST of Spg036365 vs. ExPASy TrEMBL
Match: A0A6J1CEI0 (Sphingolipid delta(4)-desaturase DES1-like OS=Momordica charantia OX=3673 GN=LOC111010944 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.4e-171
Identity = 305/412 (74.03%), Postives = 315/412 (76.46%), Query Frame = 0


Query: 29  MAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKVSFLLPDF 88
           M+  E+R EGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFK        
Sbjct: 1   MSGGEERAEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFK-------- 60

Query: 89  SLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFVFLFDLNL 148
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 149 TSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 208
                               ITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL
Sbjct: 121 --------------------ITVVVLLQLWTATTLHSADWLKILAIAYFFGSFLNHNLFL 180

Query: 209 AIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVDMDIPSQT 268
           AIHELSHNLAFSTP HNRWLGIFANLPIGVPMS+TFQKYHLEHHRFQGVDG+DMD+PS+ 
Sbjct: 181 AIHELSHNLAFSTPVHNRWLGIFANLPIGVPMSVTFQKYHLEHHRFQGVDGMDMDVPSEN 240

Query: 269 EAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMVYFWSWKS 328
           EA +VTNVV+KAIWV+FQLFFYALRPLFLKPKPPG WEFINFIIQIALDF MVYFWSWKS
Sbjct: 241 EARMVTNVVTKAIWVIFQLFFYALRPLFLKPKPPGYWEFINFIIQIALDFAMVYFWSWKS 300

Query: 329 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 388
           FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI
Sbjct: 301 FAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRI 324

Query: 389 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRM KKSE
Sbjct: 361 PGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMAKKSE 324

[Close]

BLAST of Spg036365 vs. TAIR 10
Match: AT4G04930.1 (fatty acid desaturase family protein )

HSP 1 Score: 528.9 bits (1361), Expect = 4.0e-150
Identity = 265/419 (63.25%), Postives = 296/419 (70.64%), Query Frame = 0


Query: 22  GHAQAQTMAAAEDRDEGVMATDFFWSYTDEPHASRRRQILSRYPQIRELFGPDPWAFFKV 81
           G    + +++ E+  EGVMATDFFWSYTDEPHASRRRQILS YPQIR+LFGPDPWAF K 
Sbjct: 2   GKGGREKISSNEEEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLK- 61

Query: 82  SFLLPDFSLLCVADSENLDSCGYFLVRGCFDFVSRSELGYCWSHHMSMAESDDSDLCIFV 141
                                                                       
Sbjct: 62  ------------------------------------------------------------ 121

Query: 142 FLFDLNLTSATLLHSTLLLVVHCFEGTITVVVLLQLWTATTLHSADWLKILAIAYFFGSF 201
                                      IT+VV+LQL TA  LH++ WLKIL+IAYFFGSF
Sbjct: 122 ---------------------------ITLVVILQLSTAAILHNSGWLKILSIAYFFGSF 181

Query: 202 LNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGVD 261
           LNHNLFLAIHELSHNLAFSTP +NR LGIFANLPIGVPMS+TFQKYHLEHHRFQGVDG+D
Sbjct: 182 LNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPIGVPMSVTFQKYHLEHHRFQGVDGID 241

Query: 262 MDIPSQTEAHLVTNVVSKAIWVVFQLFFYALRPLFLKPKPPGSWEFINFIIQIALDFTMV 321
           MD+P+ TEAHLVTN+ +K IWV  QLFFYALRP+F+KPKPPG WEFINF+IQI LD ++V
Sbjct: 242 MDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIFIKPKPPGYWEFINFLIQIVLDVSVV 301

Query: 322 YFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNE 381
            F+ W+SFAYLILSTFVGGGMHPMAGHFISEHYVFNP QETYSYYGPLNLLTWSVGYHNE
Sbjct: 302 LFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQETYSYYGPLNLLTWSVGYHNE 332

Query: 382 HHDFPRIPGSKLYKVKEIAPEFYNELDSYKSWSQVIYMYIMDRTVGPFSRMKRMGKKSE 441
           HHDFPRIPG+KL+ VKEIA E+Y  L+SYKSWSQVIYMYIMD TVGP+SRMKR   KS+
Sbjct: 362 HHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQVIYMYIMDTTVGPYSRMKRKLSKSD 332

The following BLAST results are available for this feature:

BLAST of Spg036365 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012427.1	4.1e-173	74.76	Sphingolipid delta(4)-desaturase DES1-like protein [Cucurbita argyrosperma subsp...	[more]
XP_022955306.1	5.4e-173	74.76	sphingolipid delta(4)-desaturase DES1-like [Cucurbita moschata] >KAG6573258.1 Sp...	[more]
XP_023542715.1	2.7e-172	74.51	sphingolipid delta(4)-desaturase DES1-like [Cucurbita pepo subsp. pepo]	[more]
XP_004146595.1	3.5e-172	74.03	sphingolipid delta(4)-desaturase DES1-like [Cucumis sativus] >KGN64668.1 hypothe...	[more]
XP_038893930.1	5.9e-172	74.51	sphingolipid delta(4)-desaturase DES1-like [Benincasa hispida]	[more]

BLAST of Spg036365 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6H5U3	4.6e-151	64.89	Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica OX=39...	[more]
Q9ZPH4	5.6e-149	63.25	Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana OX=3702 GN=At...	[more]
Q6UQ04	3.1e-83	39.85	Sphingolipid delta(4)-desaturase DES1 OS=Microtus fortis OX=100897 GN=DEGS1 PE=2...	[more]
O15121	3.5e-82	40.15	Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens OX=9606 GN=DEGS1 PE=1 SV=1	[more]
Q5RE51	2.9e-81	39.65	Sphingolipid delta(4)-desaturase DES1 OS=Pongo abelii OX=9601 GN=DEGS1 PE=2 SV=1	[more]

BLAST of Spg036365 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GTF2	2.6e-173	74.76	Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A0A0LSB9	1.7e-172	74.03	Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis sativus OX=3659 GN=Csa_1G0...	[more]
A0A6J1K2T6	4.9e-172	74.27	Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A5D3DSS5	1.4e-171	73.54	Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A6J1CEI0	1.4e-171	74.03	Sphingolipid delta(4)-desaturase DES1-like OS=Momordica charantia OX=3673 GN=LOC...	[more]

BLAST of Spg036365 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G04930.1	4.0e-150	63.25	fatty acid desaturase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013866	Sphingolipid delta4-desaturase, N-terminal	SMART	SM01269	Lipid_DES_2	coord: 39..77 e-value: 1.6E-20 score: 84.2
IPR013866	Sphingolipid delta4-desaturase, N-terminal	PFAM	PF08557	Lipid_DES	coord: 42..76 e-value: 2.1E-19 score: 68.7
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 188..406 e-value: 1.8E-21 score: 77.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..34
None	No IPR available	PANTHER	PTHR12879:SF17	SPHINGOLIPID DELTA(4)-DESATURASE DES1-LIKE	coord: 23..83
None	No IPR available	PANTHER	PTHR12879	SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2	coord: 23..83 coord: 169..439
None	No IPR available	PANTHER	PTHR12879:SF17	SPHINGOLIPID DELTA(4)-DESATURASE DES1-LIKE	coord: 169..439
IPR011388	Sphingolipid delta4-desaturase	CDD	cd03508	Delta4-sphingolipid-FADS-like	coord: 169..434 e-value: 1.58976E-159 score: 450.173

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg036365.1	Spg036365.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046513	ceramide biosynthetic process
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0030148	sphingolipid biosynthetic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042284	sphingolipid delta-4 desaturase activity