Spg036278 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg036278
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCaM_binding domain-containing protein
Locationscaffold5: 47899756 .. 47903206 (-)
RNA-Seq ExpressionSpg036278
SyntenySpg036278
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTCCATCACAACATTGAATCTTTTCCCTGACCTTTCTGCTGCAAGAAAATCACAAGGACCCTTTCTAAGGGTCTTTATGTTTTCTTTGCTTTTTTCTTCTTGTTCGTCGCAGCCTTCACAATCAGGTAACTTCCCTTTTTATGATGGTTCTGCCTTCGTTTTTGCATAATCTGAATTGGGTTTGCCTCGTCTTTGCGAGTGACTTCGATTAGTTGTTGGTATAATGCAGGATCATGTTCGGAAAAATGAACGTTTTCTAGGCAAGTCTTGTGGGTTTTCTGTGTTATGATCATAGGAATGGACATACTATCGAGTTACCAATAGGTACTTCTCTGTTCTGTTCTGTTCTGTTCTATTCGGTATATTGACTTTTAAGGAGAGATCAGCTGTTCAATGAGGTTTATTTCAGGTTTTTGTGTTCTTAATTTTCCTTGAGTAAGAGGTATTGGAAAAATGCGATTGATTTCGTTGGATATTCTTGAGTTCTGTATTTGTAAATGGAGAAATCTTTAAGGTAAGATCCCTTTTGTGGATTAAGAAGCAAGTTTTCTATTCTCATGAATATACCAATTTATGGATACGTTATGATCATGGTTGTGATTGTTTGTTTGCATTGTTTTTCTGGCTCTCTCTTGTAGTTAAGAACAAAATATATAGCCACGATATGATCATAGTTGTGATTGTTTGTTCTTGTAATTTTTTTCGCTCTTTCTTGCAGATAAGAACAAAATCATACCACTTTTCTGGAACAATGTGGCCATGCTTGTGATTGTTTGTTTGTGTCAAATTTTCGCTCTCTCTTGCGTTAAGAATGAATCATACAACACTATTGTATAGGCATGATATGACATGCCTGTGATTGTTTTTTGCGTCATTTTTTCCCTCTCTCTTGCTATGAAGAATGAAAGCATATAACACTTTAGGCACGATCTGTCCATGGTTGTGATCGTTTGTTTCCATTGTTTTGTTGCTCTCTCTCATGGTTACGAACAAAATCATAGAGCACTCCAGCCATGATATGATCATCATTGTGATTGTTTGTTGGGTTGTTTTCGGCTCTCTCTTGTAGTTAAGAGCATTTGTTCAGAATATGAATCGAAGTGCTTAAAATTTTACAATCTTCAATCCTGAAATTATTACATTTTCTTTGTCTGAGAACTTCTCATCTGGTGCAGGGTTCTGTTCAATACATTTTTCGTTTAATGGCGGAGGAGGAGAACACTGATATCGATATGCCTCTTGCTGTGGAGGTAAGTGAGGATGAAGGTAGCGAAGACAAGAGCTTTGACATCCCTGTCGTTGCAGTTACAGAGATAAGTGAGCCTGAAGATATCATTGAGGAAAGCATTGATATCATTGATATCCCTGCCTCTACGGAGGCAAATGAGCCTGAAAGTAGCAATGTAGAAGTTATTGTGGACATTAGTACCCCAAAACTTGGACGAAAGATTCCCTCTCGCTATCTCCACCCACACTCAAGCTCCTGTCATGATTTTTGCAAATATGGTGTGAAAAATGCTCTTGAAGGGAAGCCTGCAAGTCCAGTCTTGAGAAAAGATAAATCAGTTGGAGGCAATGGCCAGGATTTGAGGAGGATTATAGTTTCGCTGGCGAAACAGAACAAAGATGCAATCAGTTCAAAGTCCTCCCCAGATTATAATAAAGCCATCAATAATACTGACTTGAAGGAAGATGTAATATCTGCTCCAGAGATTGTTACTCCCTCTCCAAAAAGGCTTTTACCTTCCATCAAGGAAGTGCATGCTGCGGCAGTGCATTACAGTCGGACAAAACTTAATTTATCCCTATCAAAGGTGCCTTCTATTAAACGACAAGGCAGTTTACGAATTAAAAGAAGCAAAGAAGTTCAAAAAGGCAAGAAACAAAATGGAGATGGAAGTTCAAGCAGTCGTACAAATAGAACAAGTAGATGCCAACAGATAAATATCTCTGCAGAGGAGGATATAAAGGCCTTGGTGCCACAAGTCGTTTCTTGGACTCCAAGGACTCCAAGGCACCGTGTCGTGCGAGTTGCAATCGCAGATAAGAAAATCATTGGAAGGCGTGGCCTGAAGAACCAAAGGCATCCTAAAAAATGTAAACCTGATCCATCTATAAATGAGGTTGTAGAGGAGAAAACTATATACATGATTGAACCATCTACCAAGAATGAAACAGAGGAATCAGCTCAAAATAGTGTTGATACCACTGAGTTGTCTCGTCCACAATCTTCATCTGCAACAGACAACAGCTTGAAGCATGAACAAGAGGCAGATGGGACCCCCGTAGTGCCACCATTGTCAGTGAAAAATAACGTGAGGCGTGCTAGAAATGAAACTAAAAGTTCTAAAATTTTATCTACATCTTCACCAGTATCCGGGGGAGTTAAAGGTCTGAGACCTAAGAGATTTGGTATGACTCCAAGGTCTGAGACCCGATCGGCTCCTTCATCACCATCGTCTTCTAGGGGCCCATCTGAGCCTGTTCATGGTAAGCGTGGTGGCACTACCTCAGGAAAAGATGTGAAAAAGGGTGAAAAGTCAAAGGTGGAACACAGGATTAAGATCCAAAGGAAGGCTCTAACTGATACAGAAAACGAAGATTGCCAACCTAGAAAGCTGAAGTTTAGGAAGGGGAAGACGGTTGAGCTTCAAACTGAAACCATTACGCCAAGGAGGCTTAAATTTCGGCGTGTACGGTTACTCGGTGAGACTCATAGTCTTAAAACTGATTCAAGAAAGAGAAACATTAAGAGTAAAGAGGCCAACCAAAATGGTGATGAAGTGAAGGAAGCTGAGAATAGTTCTTTGAGACAACAAGATCAGGAAATTAAAAAGAAGAAAAGCTTTAGGAGGAAGGAAACCATCGATTATAAACTCGTTTCTAGTAGAATCAAATCTGAGAGAGTTGTCCTTAAACATCAGGATTCGAAAGGAAAGAAAGAAATCCAAAATCTGTTTAATAATGTCATTGAGGAGACAGCAAGCAAGCTTGCACAGACCAGGAAGAGTAAGGTCAAGGCTTTGGTTGGTGCCTTCGAAACAGTGATATCTCTTCAGGACACCAGACCCGCTGCAACAGTTGTTGCATGATATAAGCTGTATGTTACATATATAATATAACTTCTATGTATATTACAATCTGAAAAAGGTAATATAAATATATATAGCTTCAAATGTGACATGAATGTAAAGTTAAAAGCTTTGATCGAAATGCTATGACCTTCTATGTGGAGAAGTGTTGAGTTATTTCAATATAGCTTTTTGGAAGATTGGTTTTTCATGTGTGGTGAGGTTTTTTGTTTCTTGAAGAACAAATTACTGGTTCTCTTTTGTAGGATCTCGTTCTGTGTCATCAGAATATTTGTAATTCTAGGAATTGCAGATTTGTGCTGTTTTCTTTTTCCCACTATAATGAGTTCCAATCTTAATACTATTTACAGATCAACC

mRNA sequence

CCTTCCATCACAACATTGAATCTTTTCCCTGACCTTTCTGCTGCAAGAAAATCACAAGGACCCTTTCTAAGGGTCTTTATGTTTTCTTTGCTTTTTTCTTCTTGTTCGTCGCAGCCTTCACAATCAGATAAGAACAAAATCATACCACTTTTCTGGAACAATGTGGCCATGCTTGGTTCTGTTCAATACATTTTTCGTTTAATGGCGGAGGAGGAGAACACTGATATCGATATGCCTCTTGCTGTGGAGGTAAGTGAGGATGAAGGTAGCGAAGACAAGAGCTTTGACATCCCTGTCGTTGCAGTTACAGAGATAAGTGAGCCTGAAGATATCATTGAGGAAAGCATTGATATCATTGATATCCCTGCCTCTACGGAGGCAAATGAGCCTGAAAGTAGCAATGTAGAAGTTATTGTGGACATTAGTACCCCAAAACTTGGACGAAAGATTCCCTCTCGCTATCTCCACCCACACTCAAGCTCCTGTCATGATTTTTGCAAATATGGTGTGAAAAATGCTCTTGAAGGGAAGCCTGCAAGTCCAGTCTTGAGAAAAGATAAATCAGTTGGAGGCAATGGCCAGGATTTGAGGAGGATTATAGTTTCGCTGGCGAAACAGAACAAAGATGCAATCAGTTCAAAGTCCTCCCCAGATTATAATAAAGCCATCAATAATACTGACTTGAAGGAAGATGTAATATCTGCTCCAGAGATTGTTACTCCCTCTCCAAAAAGGCTTTTACCTTCCATCAAGGAAGTGCATGCTGCGGCAGTGCATTACAGTCGGACAAAACTTAATTTATCCCTATCAAAGGTGCCTTCTATTAAACGACAAGGCAGTTTACGAATTAAAAGAAGCAAAGAAGTTCAAAAAGGCAAGAAACAAAATGGAGATGGAAGTTCAAGCAGTCGTACAAATAGAACAAGTAGATGCCAACAGATAAATATCTCTGCAGAGGAGGATATAAAGGCCTTGGTGCCACAAGTCGTTTCTTGGACTCCAAGGACTCCAAGGCACCGTGTCGTGCGAGTTGCAATCGCAGATAAGAAAATCATTGGAAGGCGTGGCCTGAAGAACCAAAGGCATCCTAAAAAATGTAAACCTGATCCATCTATAAATGAGGTTGTAGAGGAGAAAACTATATACATGATTGAACCATCTACCAAGAATGAAACAGAGGAATCAGCTCAAAATAGTGTTGATACCACTGAGTTGTCTCGTCCACAATCTTCATCTGCAACAGACAACAGCTTGAAGCATGAACAAGAGGCAGATGGGACCCCCGTAGTGCCACCATTGTCAGTGAAAAATAACGTGAGGCGTGCTAGAAATGAAACTAAAAGTTCTAAAATTTTATCTACATCTTCACCAGTATCCGGGGGAGTTAAAGGTCTGAGACCTAAGAGATTTGGTATGACTCCAAGGTCTGAGACCCGATCGGCTCCTTCATCACCATCGTCTTCTAGGGGCCCATCTGAGCCTGTTCATGGTAAGCGTGGTGGCACTACCTCAGGAAAAGATGTGAAAAAGGGTGAAAAGTCAAAGGTGGAACACAGGATTAAGATCCAAAGGAAGGCTCTAACTGATACAGAAAACGAAGATTGCCAACCTAGAAAGCTGAAGTTTAGGAAGGGGAAGACGGTTGAGCTTCAAACTGAAACCATTACGCCAAGGAGGCTTAAATTTCGGCGTGTACGGTTACTCGGTGAGACTCATAGTCTTAAAACTGATTCAAGAAAGAGAAACATTAAGAGTAAAGAGGCCAACCAAAATGGTGATGAAGTGAAGGAAGCTGAGAATAGTTCTTTGAGACAACAAGATCAGGAAATTAAAAAGAAGAAAAGCTTTAGGAGGAAGGAAACCATCGATTATAAACTCGTTTCTAGTAGAATCAAATCTGAGAGAGTTGTCCTTAAACATCAGGATTCGAAAGGAAAGAAAGAAATCCAAAATCTGTTTAATAATGTCATTGAGGAGACAGCAAGCAAGCTTGCACAGACCAGGAAGAGTAAGGTCAAGGCTTTGGTTGGTGCCTTCGAAACAGTGATATCTCTTCAGGACACCAGACCCGCTGCAACAGTTGTTGCATGA

Coding sequence (CDS)

CCTTCCATCACAACATTGAATCTTTTCCCTGACCTTTCTGCTGCAAGAAAATCACAAGGACCCTTTCTAAGGGTCTTTATGTTTTCTTTGCTTTTTTCTTCTTGTTCGTCGCAGCCTTCACAATCAGATAAGAACAAAATCATACCACTTTTCTGGAACAATGTGGCCATGCTTGGTTCTGTTCAATACATTTTTCGTTTAATGGCGGAGGAGGAGAACACTGATATCGATATGCCTCTTGCTGTGGAGGTAAGTGAGGATGAAGGTAGCGAAGACAAGAGCTTTGACATCCCTGTCGTTGCAGTTACAGAGATAAGTGAGCCTGAAGATATCATTGAGGAAAGCATTGATATCATTGATATCCCTGCCTCTACGGAGGCAAATGAGCCTGAAAGTAGCAATGTAGAAGTTATTGTGGACATTAGTACCCCAAAACTTGGACGAAAGATTCCCTCTCGCTATCTCCACCCACACTCAAGCTCCTGTCATGATTTTTGCAAATATGGTGTGAAAAATGCTCTTGAAGGGAAGCCTGCAAGTCCAGTCTTGAGAAAAGATAAATCAGTTGGAGGCAATGGCCAGGATTTGAGGAGGATTATAGTTTCGCTGGCGAAACAGAACAAAGATGCAATCAGTTCAAAGTCCTCCCCAGATTATAATAAAGCCATCAATAATACTGACTTGAAGGAAGATGTAATATCTGCTCCAGAGATTGTTACTCCCTCTCCAAAAAGGCTTTTACCTTCCATCAAGGAAGTGCATGCTGCGGCAGTGCATTACAGTCGGACAAAACTTAATTTATCCCTATCAAAGGTGCCTTCTATTAAACGACAAGGCAGTTTACGAATTAAAAGAAGCAAAGAAGTTCAAAAAGGCAAGAAACAAAATGGAGATGGAAGTTCAAGCAGTCGTACAAATAGAACAAGTAGATGCCAACAGATAAATATCTCTGCAGAGGAGGATATAAAGGCCTTGGTGCCACAAGTCGTTTCTTGGACTCCAAGGACTCCAAGGCACCGTGTCGTGCGAGTTGCAATCGCAGATAAGAAAATCATTGGAAGGCGTGGCCTGAAGAACCAAAGGCATCCTAAAAAATGTAAACCTGATCCATCTATAAATGAGGTTGTAGAGGAGAAAACTATATACATGATTGAACCATCTACCAAGAATGAAACAGAGGAATCAGCTCAAAATAGTGTTGATACCACTGAGTTGTCTCGTCCACAATCTTCATCTGCAACAGACAACAGCTTGAAGCATGAACAAGAGGCAGATGGGACCCCCGTAGTGCCACCATTGTCAGTGAAAAATAACGTGAGGCGTGCTAGAAATGAAACTAAAAGTTCTAAAATTTTATCTACATCTTCACCAGTATCCGGGGGAGTTAAAGGTCTGAGACCTAAGAGATTTGGTATGACTCCAAGGTCTGAGACCCGATCGGCTCCTTCATCACCATCGTCTTCTAGGGGCCCATCTGAGCCTGTTCATGGTAAGCGTGGTGGCACTACCTCAGGAAAAGATGTGAAAAAGGGTGAAAAGTCAAAGGTGGAACACAGGATTAAGATCCAAAGGAAGGCTCTAACTGATACAGAAAACGAAGATTGCCAACCTAGAAAGCTGAAGTTTAGGAAGGGGAAGACGGTTGAGCTTCAAACTGAAACCATTACGCCAAGGAGGCTTAAATTTCGGCGTGTACGGTTACTCGGTGAGACTCATAGTCTTAAAACTGATTCAAGAAAGAGAAACATTAAGAGTAAAGAGGCCAACCAAAATGGTGATGAAGTGAAGGAAGCTGAGAATAGTTCTTTGAGACAACAAGATCAGGAAATTAAAAAGAAGAAAAGCTTTAGGAGGAAGGAAACCATCGATTATAAACTCGTTTCTAGTAGAATCAAATCTGAGAGAGTTGTCCTTAAACATCAGGATTCGAAAGGAAAGAAAGAAATCCAAAATCTGTTTAATAATGTCATTGAGGAGACAGCAAGCAAGCTTGCACAGACCAGGAAGAGTAAGGTCAAGGCTTTGGTTGGTGCCTTCGAAACAGTGATATCTCTTCAGGACACCAGACCCGCTGCAACAGTTGTTGCATGA

Protein sequence

PSITTLNLFPDLSAARKSQGPFLRVFMFSLLFSSCSSQPSQSDKNKIIPLFWNNVAMLGSVQYIFRLMAEEENTDIDMPLAVEVSEDEGSEDKSFDIPVVAVTEISEPEDIIEESIDIIDIPASTEANEPESSNVEVIVDISTPKLGRKIPSRYLHPHSSSCHDFCKYGVKNALEGKPASPVLRKDKSVGGNGQDLRRIIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDVISAPEIVTPSPKRLLPSIKEVHAAAVHYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKGKKQNGDGSSSSRTNRTSRCQQINISAEEDIKALVPQVVSWTPRTPRHRVVRVAIADKKIIGRRGLKNQRHPKKCKPDPSINEVVEEKTIYMIEPSTKNETEESAQNSVDTTELSRPQSSSATDNSLKHEQEADGTPVVPPLSVKNNVRRARNETKSSKILSTSSPVSGGVKGLRPKRFGMTPRSETRSAPSSPSSSRGPSEPVHGKRGGTTSGKDVKKGEKSKVEHRIKIQRKALTDTENEDCQPRKLKFRKGKTVELQTETITPRRLKFRRVRLLGETHSLKTDSRKRNIKSKEANQNGDEVKEAENSSLRQQDQEIKKKKSFRRKETIDYKLVSSRIKSERVVLKHQDSKGKKEIQNLFNNVIEETASKLAQTRKSKVKALVGAFETVISLQDTRPAATVVA
Homology
BLAST of Spg036278 vs. NCBI nr
Match: XP_038894083.1 (uncharacterized protein LOC120082825 [Benincasa hispida] >XP_038894084.1 uncharacterized protein LOC120082825 [Benincasa hispida])

HSP 1 Score: 805.4 bits (2079), Expect = 3.7e-229
Identity = 465/618 (75.24%), Postives = 509/618 (82.36%), Query Frame = 0

Query: 83  EVSEDEGSEDKSFDIPVVAVTEISEPEDIIEESIDIIDIPAST--EANEPESSNVEVIVD 142
           EVSEDE S+++SFDIPV+AV E SEPEDIIEE+IDIIDIPA T  E NEPES +VEVIVD
Sbjct: 44  EVSEDEASQEESFDIPVIAVAETSEPEDIIEENIDIIDIPAITTIELNEPESRSVEVIVD 103

Query: 143 I--STPKLGRKIPSRYLHPHSSSCHDFCKYGVKNALEGKPASPVLRKDKSVGGNGQDLRR 202
           I  STPK+  KI SRYL PH+ SCHDFCKYG K+ LEGKPAS VLRK KS GG+G+ LRR
Sbjct: 104 INSSTPKIMPKILSRYLFPHTGSCHDFCKYGTKHGLEGKPASTVLRKVKSAGGDGRGLRR 163

Query: 203 IIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDVISAPEIVTPSPKRLLPSIKEVHAAAV 262
           IIVS AKQNKDA S KSSP++N       LKED+IS PEIVTPSPKRLLPSIKEV AAAV
Sbjct: 164 IIVSSAKQNKDATSPKSSPEHNPINVTGHLKEDIISPPEIVTPSPKRLLPSIKEVQAAAV 223

Query: 263 HYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKGKKQNGDGSSSSRTNRTSRCQQINISA 322
           HYSRTKLNLSLSK  S   QGS R KR+KE+++G K++GDGSSSS TN TSR Q++NISA
Sbjct: 224 HYSRTKLNLSLSKASSFAGQGSSRTKRNKEIRRGNKKDGDGSSSSCTNSTSRSQEMNISA 283

Query: 323 EEDIKALVPQVVSWTPRTPRHRVVRVAIADKKIIGRRGLKNQRHPKKCKPDPSINEVVEE 382
           EEDIKALVP+VVSW   TPR+RV RVAI DKKIIGR GLK+Q H  KCKPDPS NE VEE
Sbjct: 284 EEDIKALVPEVVSW---TPRNRVKRVAIPDKKIIGRSGLKSQSHSIKCKPDPSNNEDVEE 343

Query: 383 KTIYMIEPSTKNETEESAQNSVDTTELSRPQSSSATDNSLKHEQEADGTPVVPPLSVKNN 442
           KT+YMIEPSTKNETEE AQNSV  TE SRPQSSSATDNSLKHE+E D    +PPLSVK N
Sbjct: 344 KTLYMIEPSTKNETEEMAQNSVHATESSRPQSSSATDNSLKHEKEVDENLKMPPLSVKKN 403

Query: 443 VRRARNETKSSKILSTSSPVSGGVKGLRPKRFGMTPRSETRSAPSSPSSSRGPSEPVHGK 502
           V R      SSKI S S PVS   KG+RPKRFGM  RSETR APSSP SSR P EPVH +
Sbjct: 404 VVRCARNVTSSKISSASPPVSKVFKGIRPKRFGMVQRSETRLAPSSPLSSRPPFEPVHVE 463

Query: 503 RGGTTSGKDVKKGEKSKVEHRIKIQRKALTDTENEDCQPRKLKFRKGKTVELQTETITPR 562
             G+TSG +VKK E S+V+HR+K +R  LTD+EN D Q RKLKFRKG+ VELQ ET TPR
Sbjct: 464 HRGSTSGNEVKKRENSEVKHRLKTKRMTLTDSENGDSQSRKLKFRKGRVVELQAETNTPR 523

Query: 563 RLKFRRVRLLGETHSLKTDSRKRNIKSKEANQNGDEVKEAENSSLRQQDQEIKKKKSFRR 622
           RLKFRRV LLGET S K D RKRNIK KEANQNG+EVKEA+NSSLRQQDQE+KKK+SFRR
Sbjct: 524 RLKFRRVHLLGETQSPKGDPRKRNIKGKEANQNGNEVKEADNSSLRQQDQELKKKRSFRR 583

Query: 623 KETIDYKLVSSRIKSERVVLKHQDSKGKKEIQNLFNNVIEETASKLAQTRKSKVKALVGA 682
           KETID KLVSSRIKSERVVL+HQDS+GKK IQNLFNNVIEETASKLAQTRKSKVKALVGA
Sbjct: 584 KETIDGKLVSSRIKSERVVLRHQDSRGKKAIQNLFNNVIEETASKLAQTRKSKVKALVGA 643

Query: 683 FETVISLQDTRPAATVVA 697
           FETVISLQDTRP AT VA
Sbjct: 644 FETVISLQDTRPVATSVA 658

BLAST of Spg036278 vs. NCBI nr
Match: XP_008446785.1 (PREDICTED: uncharacterized protein LOC103489380 [Cucumis melo] >XP_008446868.1 PREDICTED: uncharacterized protein LOC103489380 [Cucumis melo] >XP_008446947.1 PREDICTED: uncharacterized protein LOC103489380 [Cucumis melo] >XP_008447016.1 PREDICTED: uncharacterized protein LOC103489380 [Cucumis melo] >TYJ98916.1 CaM_binding domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 749.2 bits (1933), Expect = 3.2e-212
Identity = 452/630 (71.75%), Postives = 508/630 (80.63%), Query Frame = 0

Query: 71  EENTDIDMPLAV-EVSEDEGSEDKSFDIPVVAVTEISEPEDIIEES---IDIIDIPASTE 130
           +EN+DI  PLA+ EVSE E S+++SFDIPVVAV  ISEPEDI EES   IDIIDIPA+ E
Sbjct: 3   DENSDI--PLAMEEVSEAEVSQEESFDIPVVAVANISEPEDITEESIDIIDIIDIPATIE 62

Query: 131 ANEPESSNVEVIVDI--STPKLGRKIPSRYLHPHSSSCHDFCKYGVKNALEGKPASPVLR 190
            NEPES  VEVIVD   +TPK+  K+ SRYL PH+ SCHDFCKYG ++ALEGKPASPV R
Sbjct: 63  VNEPESCKVEVIVDTDSNTPKIRPKVLSRYLRPHTGSCHDFCKYGSEHALEGKPASPVSR 122

Query: 191 KDKSVGGNGQDLRRIIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDVISAPEIVTPSPK 250
           K K VGGN QDLRR IVSLAKQNK++ S KSS +YN  IN TDLKED+IS+PEIVTP PK
Sbjct: 123 KAKLVGGNSQDLRRTIVSLAKQNKESNSPKSSQEYN-PINITDLKEDIISSPEIVTP-PK 182

Query: 251 RLLPSIKEVHAAAVHYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKGKKQNGDGSSSSR 310
           RLLPS KEV AAAVHYSRTKLNLS SKV S   QGS R KR+KE++KGKK++GDGS SS 
Sbjct: 183 RLLPSTKEVQAAAVHYSRTKLNLSSSKVSSFAGQGSSRTKRNKEIRKGKKKDGDGSLSS- 242

Query: 311 TNRTSRCQQINISAEEDIKALVPQVVSWTPRTPRHRVVRVAIADKKIIGRRGLKNQRHPK 370
           +N TSR  ++NISAEEDI ALVP+V S   RTP+ RV RVAIADKK IGR GLK+Q H  
Sbjct: 243 SNSTSRSLEMNISAEEDITALVPEVGS---RTPKTRVKRVAIADKKSIGRNGLKSQIHHI 302

Query: 371 KCKPDPSINEVVEEKTIYMIEPSTKNETEESAQNSVDTTELSRPQSSSATDNSLKHEQEA 430
           KCKPDPS NE VEEKT+YMIEPS+KNETE  +Q+S+ TTE S+PQSSS TDN+LKHEQEA
Sbjct: 303 KCKPDPSNNEDVEEKTLYMIEPSSKNETEGMSQSSLHTTESSQPQSSSTTDNNLKHEQEA 362

Query: 431 DGTPVVPPLSVKNN-VRRARNETKSSKILSTSSPVSGGVKGLRPKRFGMTPRSETRSAPS 490
               +VPP+S K N V+RARN T S KILSTS  V    KG+RPKRFGM  RSETRSAPS
Sbjct: 363 VANSIVPPMSEKKNVVKRARNGT-SPKILSTSPTV---FKGIRPKRFGMVQRSETRSAPS 422

Query: 491 SPSSSRGPSEPVHGKRGGTTSGKDVKKGEKSKVEHRIKIQRKALTDTENEDCQPRKLKFR 550
           SP SSR  SEP+H +  G+TSG  VKK E SKV+HR+K +R  LTD+EN DCQ RKLKFR
Sbjct: 423 SPLSSRSQSEPIHVEHRGSTSGNGVKKIENSKVDHRLKTRRMTLTDSENGDCQSRKLKFR 482

Query: 551 KGKTVELQTETITPRRLKFRRVRLLGETHSLKTDSRKRNIKSKEANQNGDEVKEAENSSL 610
           KG+ VELQ ET TPRRLKFRRVRLLGE  S K DSRKRNIK KE NQNG EVKE ENSSL
Sbjct: 483 KGRMVELQPETSTPRRLKFRRVRLLGEAESPKGDSRKRNIKGKEGNQNGKEVKEGENSSL 542

Query: 611 RQQDQEIKKKKSFRRKETIDYKLVSSRIKSERVVLKHQDSKGKKEIQNLFNNVIEETASK 670
           RQQD+++KKK+SFR     D KLVSSR KSERVVL+HQDSKGKKE+ NLFNNVIEETASK
Sbjct: 543 RQQDKDLKKKRSFR-----DGKLVSSRFKSERVVLRHQDSKGKKEVLNLFNNVIEETASK 602

Query: 671 LAQTRKSKVKALVGAFETVISLQDTRPAAT 694
           LA+TRKSKVKALVGAFETVISLQDT+P AT
Sbjct: 603 LAKTRKSKVKALVGAFETVISLQDTKPTAT 615

BLAST of Spg036278 vs. NCBI nr
Match: XP_011650019.1 (uncharacterized protein LOC105434693 [Cucumis sativus] >XP_011650022.1 uncharacterized protein LOC105434693 [Cucumis sativus] >KGN64029.1 hypothetical protein Csa_013389 [Cucumis sativus])

HSP 1 Score: 745.7 bits (1924), Expect = 3.5e-211
Identity = 445/642 (69.31%), Postives = 508/642 (79.13%), Query Frame = 0

Query: 73  NTDIDMPLAV-EVSEDEGSEDKSFDIPVVAVTEISEPEDIIEESIDII------------ 132
           N + D+PLA+ EVSE E S+++SFDIPV+AV  ISEPEDI EE IDII            
Sbjct: 3   NENSDIPLAMEEVSEAEVSQEESFDIPVIAVANISEPEDITEEIIDIIDIIDIPATIEVN 62

Query: 133 -----DIPASTEANEPESSNVEVIVDIST--PKLGRKIPSRYLHPHSSSCHDFCKYGVKN 192
                DIPA+ E +EPES  VEVI+DI++  PK+  ++ SRYL P++ SCHDFCKYG K+
Sbjct: 63  EPDIVDIPATIEVSEPESCKVEVIMDINSNIPKIRPRVLSRYLLPYTGSCHDFCKYGSKH 122

Query: 193 ALEGKPASPVLRKDKSVGGNGQDLRRIIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDV 252
            LEGKPASP+ RK K VGGNGQDLRR +VSLAKQNK++ S KSS +YN + N TDLKED+
Sbjct: 123 DLEGKPASPISRKAKLVGGNGQDLRRTVVSLAKQNKESNSRKSSLEYNPS-NVTDLKEDI 182

Query: 253 ISAPEIVTPSPKRLLPSIKEVHAAAVHYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKG 312
           IS+PEIVTPSPKRLLPS KEV AAAVHYSRTKLNLSLSKV S   QG  R KR+KE++KG
Sbjct: 183 ISSPEIVTPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAGQGGSRTKRNKEIRKG 242

Query: 313 KKQNGDGSSSSRTNRTSRCQQINISAEEDIKALVPQVVSWTPRTPRHRVVRVAIADKKII 372
           KK+ GDGS SS +N TSR  ++N+SAEEDI ALVP+V S   RTPR RV RVAIADKK I
Sbjct: 243 KKKEGDGSLSS-SNSTSRSLEMNVSAEEDITALVPEVGS---RTPRTRVKRVAIADKKNI 302

Query: 373 GRRGLKNQRHPKKCKPDPSINEVVEEKTIYMIEPSTKNETEESAQNSVDTTELSRPQSSS 432
           GR GLK+Q HP KCKPDPS NE VEEKT+YMIEPSTK+ETEE +QNSV TTE S+PQSSS
Sbjct: 303 GRNGLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKDETEEISQNSVHTTESSQPQSSS 362

Query: 433 ATDNSLKHEQEADGTPVVPPLSVKNN-VRRARNETKSSKILSTSSPVSGGVKGLRPKRFG 492
            TDN+LKHEQEA    +VPP+SVK N V+RARN T S+KIL TS   S   KG+RPKRFG
Sbjct: 363 TTDNNLKHEQEAAANSIVPPMSVKKNVVKRARNGT-SAKILCTSPTASKVFKGIRPKRFG 422

Query: 493 MTPRSETRSAPSSPSSSRGPSEPVHGKRGGTTSGKDVKKGEKSKVEHRIKIQRKALTDTE 552
           M  RSETRSAPSSP SSR  SEP+H +  G+TSG DVKK E SKV+HR+K +   LTD+E
Sbjct: 423 MVQRSETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTKGMTLTDSE 482

Query: 553 NEDCQPRKLKFRKGKTVELQTETITPRRLKFRRVRLLGETHSLKTDSRKRNIKSKEANQN 612
           N DCQ RKLKFRKGKTVELQ ET +PRRLKFR VRLLGET S K DSRKRNI  K+ NQN
Sbjct: 483 NGDCQSRKLKFRKGKTVELQPETSSPRRLKFRHVRLLGETQSPKGDSRKRNIMGKDGNQN 542

Query: 613 GDEVKEAENSSLRQQDQEIKKKKSFRRKETIDYKLVSSRIKSERVVLKHQDSKGKKEIQN 672
           G   KE ENSSLRQQD+++KKK+SFR     D KL+SSR KSERVVL+HQDSKGKKEI N
Sbjct: 543 G---KEGENSSLRQQDKDLKKKRSFR-----DGKLISSRFKSERVVLRHQDSKGKKEILN 602

Query: 673 LFNNVIEETASKLAQTRKSKVKALVGAFETVISLQDTRPAAT 694
           LFNNVIEETASKLA+TRKSKVKALVGAFETVISLQDT+PAAT
Sbjct: 603 LFNNVIEETASKLAKTRKSKVKALVGAFETVISLQDTKPAAT 630

BLAST of Spg036278 vs. NCBI nr
Match: XP_022998278.1 (uncharacterized protein LOC111492963 [Cucurbita maxima])

HSP 1 Score: 698.4 bits (1801), Expect = 6.4e-197
Identity = 429/640 (67.03%), Postives = 474/640 (74.06%), Query Frame = 0

Query: 72  ENTDIDMPLAVEVSEDEGSEDKSFDIPVVAVTEISEPEDIIEESIDI------------- 131
           ENTDI  PL VEVS DEG + +SFDIP     E++EP  IIE S DI             
Sbjct: 4   ENTDI--PLVVEVSGDEGRQHESFDIP--TNMEVNEPNAIIEGSTDISLALEASADEGSQ 63

Query: 132 ---IDIPASTEANEPESSNVEVIVDISTPKLGRKIPSRYLHPHSSSCHDFCKYGVKNALE 191
               DIP + E NEPES NV VIV+I+TPK  R  P RYL P + SCHDFCKYG K+ +E
Sbjct: 64  DEKFDIPTTMEVNEPESCNVGVIVNINTPKFRRIFP-RYLSPRTGSCHDFCKYGTKHPIE 123

Query: 192 GKPASPVLRKDKSVGGNGQDLRRIIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDVISA 251
             PAS VLRK KSVG + +DLRRI V+LAK N D +S K S DY+  IN TDLKEDV S+
Sbjct: 124 RNPASAVLRKGKSVGRDVRDLRRIRVTLAKPNNDTVSPKFSKDYD-GINITDLKEDVTSS 183

Query: 252 PEIVTPSPKRLLPSIKEVHAAAVHYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKGKKQ 311
           PEI+ PSPK+ LP IKEV AAAV YSRTKLNLSLSK  S   Q + R  R+KEV++ KKQ
Sbjct: 184 PEIINPSPKKRLPPIKEVEAAAVQYSRTKLNLSLSKASSSAGQCTSRTNRNKEVRRSKKQ 243

Query: 312 NGDGSSSSRTNRTSRCQQINISAEEDIKALVPQVVSWTPRTPRHRVVRVAIADKKIIGRR 371
           +G GSSSS T+ TSRCQ+I IS   D KALVP  VSW   TPR+RV RVAI DKKIIGR 
Sbjct: 244 DGYGSSSSSTDSTSRCQEIKISTVGDRKALVPHAVSW---TPRNRVKRVAIVDKKIIGRH 303

Query: 372 GLKNQRHPKKCKPDPSINEVVEEKTIYMIEPSTKNETEESAQNSVDTTELSRPQSSSATD 431
            LKNQ   KK KPDPS +EVVEEKT+YMIEPSTK ETE  AQNSV  TELS PQSSSATD
Sbjct: 304 SLKNQSRLKKIKPDPSNSEVVEEKTLYMIEPSTKKETEGPAQNSVHFTELSHPQSSSATD 363

Query: 432 NSLKHEQEADGTPVVPPLSVKNNVRRARNETKSSKILSTSSPVSGGVKGLRPKRFGMTPR 491
           NS KH+QEADGT + P L V+ N RR RN T SSK LSTS  V  G KGLRPKRF M   
Sbjct: 364 NSFKHKQEADGTIITPHLLVEENTRRTRNGT-SSKSLSTSPTVFKGFKGLRPKRFDMIQM 423

Query: 492 SETRSAPSSPSSSRGPSEPVHGKRGGTTSGKDVKKGEKSKVEHRIKIQRKALTDTENEDC 551
           SETRSAPSSPSSSR  SEPVH              GEKSKVEH+IK +RK LTD+EN DC
Sbjct: 424 SETRSAPSSPSSSRCSSEPVH--------------GEKSKVEHKIKARRKTLTDSENGDC 483

Query: 552 QPRKLKFRKGKTVELQTETITPRRLKFRRVRLLGETHSLKTDSRKRNIKSKEANQNGDEV 611
           Q RKL FRKG+ VELQTE ITPRRL F+RVR L ET S K+DSRKR I+ KEANQNGDEV
Sbjct: 484 QSRKLHFRKGRMVELQTEPITPRRLIFKRVRSLSETQSPKSDSRKRIIQRKEANQNGDEV 543

Query: 612 KEAENSSLRQQDQEIKKKKSFRRKETIDYKLVSSRIKSERVVLKHQDSKGKKEIQNLFNN 671
           KE ENSSLRQQDQE K+KKSFRR+ETID KLVSSR+KSER+VLKHQDS  K EIQ L NN
Sbjct: 544 KETENSSLRQQDQEFKRKKSFRRQETIDGKLVSSRLKSERIVLKHQDSNEKIEIQKLLNN 603

Query: 672 VIEETASKLAQTRKSKVKALVGAFETVISLQDTRPAATVV 696
           VIEETA+KLA+TRKSKVKALVGAFETVISLQD +PAATVV
Sbjct: 604 VIEETATKLAKTRKSKVKALVGAFETVISLQDAKPAATVV 619

BLAST of Spg036278 vs. NCBI nr
Match: XP_022137059.1 (uncharacterized protein LOC111008620 [Momordica charantia])

HSP 1 Score: 694.1 bits (1790), Expect = 1.2e-195
Identity = 443/739 (59.95%), Postives = 504/739 (68.20%), Query Frame = 0

Query: 71  EENTDIDMPLAVEVSEDEGSEDKSFDIPVVAVTEISEPEDIIEESIDIIDIPASTEANEP 130
           EENT I  P+AVE S DEGS+D+SFDIPV+AV EISEP+DI EESID  DI A  E NE 
Sbjct: 3   EENTGI--PVAVETSGDEGSQDESFDIPVIAVAEISEPDDISEESID--DILAIPEENES 62

Query: 131 ESSNVEVIVDISTPKLGRKIPSRYLHPHSSSCHDFCKYGVKNALEGKPASPVLRKDKSVG 190
           E S+VEVIVDI  PK+GRKIPSRYL P++ SCHDFCKYG K+ LEGKPAS VL+K  +VG
Sbjct: 63  ERSSVEVIVDIDAPKIGRKIPSRYLLPYAGSCHDFCKYGTKHPLEGKPASLVLKKVITVG 122

Query: 191 GNGQDLRRIIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDVISAPEIVTPSPKRLLPSI 250
           G+G+D RR ++SLAKQNKD I++KSSPDYN A+NNTD KED+I +PEIV  SPKRLLPS 
Sbjct: 123 GDGRDSRRTMISLAKQNKDPINTKSSPDYN-AMNNTDSKEDIIYSPEIVILSPKRLLPSS 182

Query: 251 KEVHAAAVHYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKGKKQNGDGSSSSRTN-RTS 310
           KE+ AAAVHYSRTKLNLS SK  S   QGS   KR+KEVQ+G++ +GDGS SSRTN  TS
Sbjct: 183 KEMQAAAVHYSRTKLNLSHSKRSSFSGQGSSGTKRNKEVQEGQELDGDGSISSRTNSSTS 242

Query: 311 RCQQINISAEEDIKALVPQVVSWTPRTPRHRVVRVAIADKKIIGRRGLKNQRHPKKCKPD 370
           RCQ+I+I  EED+KALVPQ +S   R+PR+   RVA+ADKK+ GRR LKN+ HP K K  
Sbjct: 243 RCQEIDIFEEEDMKALVPQAIS---RSPRNHGKRVAVADKKVTGRRRLKNRSHPMKTKLV 302

Query: 371 PSINEVVEEKTIYMIEPSTKNETEESAQNSVDTTELSRPQSSSATDNSLKHEQEADGTPV 430
              NE VEEKT+YMIEPSTKNE EE AQNSV TT+LSRP+SSSA DN LKH+QEADGT  
Sbjct: 303 HCKNEDVEEKTLYMIEPSTKNEAEELAQNSVHTTDLSRPKSSSAEDNGLKHDQEADGTSK 362

Query: 431 VPPLSVKNNVRRARNETKSSKILSTSSPVSGGVKGLRPKRFGMTPRSETRSAPSSPSSSR 490
           VP  SV+    RARN T SSK L TSS VS G KGLRPKR+GM  RS+ +SAPSS S SR
Sbjct: 363 VPQ-SVEKIASRARNGT-SSKNLFTSSSVSRGSKGLRPKRYGMIQRSKIQSAPSSSSLSR 422

Query: 491 GPSEPVHGKRGGTTSGKDVKKGEKSKVEHRIKIQR------------------------- 550
            P+EPVHG+ GG  SGKDVKK E SKVEH+IK +R                         
Sbjct: 423 YPAEPVHGEHGGRPSGKDVKKSEHSKVEHKIKTRRTTVYDSENGDFQSRKLKPRKGRLVE 482

Query: 551 ------------------------------------------------------------ 610
                                                                       
Sbjct: 483 LQPENNSPRRPKFLSDTENGDSQSRKLKLRKGRLVELQPENNSLRGPKFLSDSENGDSQS 542

Query: 611 -----------------------KALTDTENEDCQPRKLKFRKGKTVELQTETITPRRLK 670
                                  K L+D+EN D Q RKLKFR+G+ VELQ E   PRRLK
Sbjct: 543 RKLKLRKGRLVELQPENNSPRSPKFLSDSENGDVQSRKLKFREGRLVELQPENNAPRRLK 602

Query: 671 FRRVRLLGE--THSLKTDSRKRNIKSKEA----NQNGDEVKEAENSSLRQQDQEIKKKKS 695
           FRR RLLGE  T S K+DSR+RN + KEA    NQNGDE KE E+ S RQQDQE K+KKS
Sbjct: 603 FRRARLLGETQTQSPKSDSRRRNFQGKEANQNGNQNGDEAKETEHGSSRQQDQETKRKKS 662

BLAST of Spg036278 vs. ExPASy TrEMBL
Match: A0A1S3BFX3 (uncharacterized protein LOC103489380 OS=Cucumis melo OX=3656 GN=LOC103489380 PE=4 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 1.5e-212
Identity = 452/630 (71.75%), Postives = 508/630 (80.63%), Query Frame = 0

Query: 71  EENTDIDMPLAV-EVSEDEGSEDKSFDIPVVAVTEISEPEDIIEES---IDIIDIPASTE 130
           +EN+DI  PLA+ EVSE E S+++SFDIPVVAV  ISEPEDI EES   IDIIDIPA+ E
Sbjct: 3   DENSDI--PLAMEEVSEAEVSQEESFDIPVVAVANISEPEDITEESIDIIDIIDIPATIE 62

Query: 131 ANEPESSNVEVIVDI--STPKLGRKIPSRYLHPHSSSCHDFCKYGVKNALEGKPASPVLR 190
            NEPES  VEVIVD   +TPK+  K+ SRYL PH+ SCHDFCKYG ++ALEGKPASPV R
Sbjct: 63  VNEPESCKVEVIVDTDSNTPKIRPKVLSRYLRPHTGSCHDFCKYGSEHALEGKPASPVSR 122

Query: 191 KDKSVGGNGQDLRRIIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDVISAPEIVTPSPK 250
           K K VGGN QDLRR IVSLAKQNK++ S KSS +YN  IN TDLKED+IS+PEIVTP PK
Sbjct: 123 KAKLVGGNSQDLRRTIVSLAKQNKESNSPKSSQEYN-PINITDLKEDIISSPEIVTP-PK 182

Query: 251 RLLPSIKEVHAAAVHYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKGKKQNGDGSSSSR 310
           RLLPS KEV AAAVHYSRTKLNLS SKV S   QGS R KR+KE++KGKK++GDGS SS 
Sbjct: 183 RLLPSTKEVQAAAVHYSRTKLNLSSSKVSSFAGQGSSRTKRNKEIRKGKKKDGDGSLSS- 242

Query: 311 TNRTSRCQQINISAEEDIKALVPQVVSWTPRTPRHRVVRVAIADKKIIGRRGLKNQRHPK 370
           +N TSR  ++NISAEEDI ALVP+V S   RTP+ RV RVAIADKK IGR GLK+Q H  
Sbjct: 243 SNSTSRSLEMNISAEEDITALVPEVGS---RTPKTRVKRVAIADKKSIGRNGLKSQIHHI 302

Query: 371 KCKPDPSINEVVEEKTIYMIEPSTKNETEESAQNSVDTTELSRPQSSSATDNSLKHEQEA 430
           KCKPDPS NE VEEKT+YMIEPS+KNETE  +Q+S+ TTE S+PQSSS TDN+LKHEQEA
Sbjct: 303 KCKPDPSNNEDVEEKTLYMIEPSSKNETEGMSQSSLHTTESSQPQSSSTTDNNLKHEQEA 362

Query: 431 DGTPVVPPLSVKNN-VRRARNETKSSKILSTSSPVSGGVKGLRPKRFGMTPRSETRSAPS 490
               +VPP+S K N V+RARN T S KILSTS  V    KG+RPKRFGM  RSETRSAPS
Sbjct: 363 VANSIVPPMSEKKNVVKRARNGT-SPKILSTSPTV---FKGIRPKRFGMVQRSETRSAPS 422

Query: 491 SPSSSRGPSEPVHGKRGGTTSGKDVKKGEKSKVEHRIKIQRKALTDTENEDCQPRKLKFR 550
           SP SSR  SEP+H +  G+TSG  VKK E SKV+HR+K +R  LTD+EN DCQ RKLKFR
Sbjct: 423 SPLSSRSQSEPIHVEHRGSTSGNGVKKIENSKVDHRLKTRRMTLTDSENGDCQSRKLKFR 482

Query: 551 KGKTVELQTETITPRRLKFRRVRLLGETHSLKTDSRKRNIKSKEANQNGDEVKEAENSSL 610
           KG+ VELQ ET TPRRLKFRRVRLLGE  S K DSRKRNIK KE NQNG EVKE ENSSL
Sbjct: 483 KGRMVELQPETSTPRRLKFRRVRLLGEAESPKGDSRKRNIKGKEGNQNGKEVKEGENSSL 542

Query: 611 RQQDQEIKKKKSFRRKETIDYKLVSSRIKSERVVLKHQDSKGKKEIQNLFNNVIEETASK 670
           RQQD+++KKK+SFR     D KLVSSR KSERVVL+HQDSKGKKE+ NLFNNVIEETASK
Sbjct: 543 RQQDKDLKKKRSFR-----DGKLVSSRFKSERVVLRHQDSKGKKEVLNLFNNVIEETASK 602

Query: 671 LAQTRKSKVKALVGAFETVISLQDTRPAAT 694
           LA+TRKSKVKALVGAFETVISLQDT+P AT
Sbjct: 603 LAKTRKSKVKALVGAFETVISLQDTKPTAT 615

BLAST of Spg036278 vs. ExPASy TrEMBL
Match: A0A5D3BIK3 (CaM_binding domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001130 PE=4 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 1.5e-212
Identity = 452/630 (71.75%), Postives = 508/630 (80.63%), Query Frame = 0

Query: 71  EENTDIDMPLAV-EVSEDEGSEDKSFDIPVVAVTEISEPEDIIEES---IDIIDIPASTE 130
           +EN+DI  PLA+ EVSE E S+++SFDIPVVAV  ISEPEDI EES   IDIIDIPA+ E
Sbjct: 3   DENSDI--PLAMEEVSEAEVSQEESFDIPVVAVANISEPEDITEESIDIIDIIDIPATIE 62

Query: 131 ANEPESSNVEVIVDI--STPKLGRKIPSRYLHPHSSSCHDFCKYGVKNALEGKPASPVLR 190
            NEPES  VEVIVD   +TPK+  K+ SRYL PH+ SCHDFCKYG ++ALEGKPASPV R
Sbjct: 63  VNEPESCKVEVIVDTDSNTPKIRPKVLSRYLRPHTGSCHDFCKYGSEHALEGKPASPVSR 122

Query: 191 KDKSVGGNGQDLRRIIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDVISAPEIVTPSPK 250
           K K VGGN QDLRR IVSLAKQNK++ S KSS +YN  IN TDLKED+IS+PEIVTP PK
Sbjct: 123 KAKLVGGNSQDLRRTIVSLAKQNKESNSPKSSQEYN-PINITDLKEDIISSPEIVTP-PK 182

Query: 251 RLLPSIKEVHAAAVHYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKGKKQNGDGSSSSR 310
           RLLPS KEV AAAVHYSRTKLNLS SKV S   QGS R KR+KE++KGKK++GDGS SS 
Sbjct: 183 RLLPSTKEVQAAAVHYSRTKLNLSSSKVSSFAGQGSSRTKRNKEIRKGKKKDGDGSLSS- 242

Query: 311 TNRTSRCQQINISAEEDIKALVPQVVSWTPRTPRHRVVRVAIADKKIIGRRGLKNQRHPK 370
           +N TSR  ++NISAEEDI ALVP+V S   RTP+ RV RVAIADKK IGR GLK+Q H  
Sbjct: 243 SNSTSRSLEMNISAEEDITALVPEVGS---RTPKTRVKRVAIADKKSIGRNGLKSQIHHI 302

Query: 371 KCKPDPSINEVVEEKTIYMIEPSTKNETEESAQNSVDTTELSRPQSSSATDNSLKHEQEA 430
           KCKPDPS NE VEEKT+YMIEPS+KNETE  +Q+S+ TTE S+PQSSS TDN+LKHEQEA
Sbjct: 303 KCKPDPSNNEDVEEKTLYMIEPSSKNETEGMSQSSLHTTESSQPQSSSTTDNNLKHEQEA 362

Query: 431 DGTPVVPPLSVKNN-VRRARNETKSSKILSTSSPVSGGVKGLRPKRFGMTPRSETRSAPS 490
               +VPP+S K N V+RARN T S KILSTS  V    KG+RPKRFGM  RSETRSAPS
Sbjct: 363 VANSIVPPMSEKKNVVKRARNGT-SPKILSTSPTV---FKGIRPKRFGMVQRSETRSAPS 422

Query: 491 SPSSSRGPSEPVHGKRGGTTSGKDVKKGEKSKVEHRIKIQRKALTDTENEDCQPRKLKFR 550
           SP SSR  SEP+H +  G+TSG  VKK E SKV+HR+K +R  LTD+EN DCQ RKLKFR
Sbjct: 423 SPLSSRSQSEPIHVEHRGSTSGNGVKKIENSKVDHRLKTRRMTLTDSENGDCQSRKLKFR 482

Query: 551 KGKTVELQTETITPRRLKFRRVRLLGETHSLKTDSRKRNIKSKEANQNGDEVKEAENSSL 610
           KG+ VELQ ET TPRRLKFRRVRLLGE  S K DSRKRNIK KE NQNG EVKE ENSSL
Sbjct: 483 KGRMVELQPETSTPRRLKFRRVRLLGEAESPKGDSRKRNIKGKEGNQNGKEVKEGENSSL 542

Query: 611 RQQDQEIKKKKSFRRKETIDYKLVSSRIKSERVVLKHQDSKGKKEIQNLFNNVIEETASK 670
           RQQD+++KKK+SFR     D KLVSSR KSERVVL+HQDSKGKKE+ NLFNNVIEETASK
Sbjct: 543 RQQDKDLKKKRSFR-----DGKLVSSRFKSERVVLRHQDSKGKKEVLNLFNNVIEETASK 602

Query: 671 LAQTRKSKVKALVGAFETVISLQDTRPAAT 694
           LA+TRKSKVKALVGAFETVISLQDT+P AT
Sbjct: 603 LAKTRKSKVKALVGAFETVISLQDTKPTAT 615

BLAST of Spg036278 vs. ExPASy TrEMBL
Match: A0A0A0LST4 (CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G038960 PE=4 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 1.7e-211
Identity = 445/642 (69.31%), Postives = 508/642 (79.13%), Query Frame = 0

Query: 73  NTDIDMPLAV-EVSEDEGSEDKSFDIPVVAVTEISEPEDIIEESIDII------------ 132
           N + D+PLA+ EVSE E S+++SFDIPV+AV  ISEPEDI EE IDII            
Sbjct: 3   NENSDIPLAMEEVSEAEVSQEESFDIPVIAVANISEPEDITEEIIDIIDIIDIPATIEVN 62

Query: 133 -----DIPASTEANEPESSNVEVIVDIST--PKLGRKIPSRYLHPHSSSCHDFCKYGVKN 192
                DIPA+ E +EPES  VEVI+DI++  PK+  ++ SRYL P++ SCHDFCKYG K+
Sbjct: 63  EPDIVDIPATIEVSEPESCKVEVIMDINSNIPKIRPRVLSRYLLPYTGSCHDFCKYGSKH 122

Query: 193 ALEGKPASPVLRKDKSVGGNGQDLRRIIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDV 252
            LEGKPASP+ RK K VGGNGQDLRR +VSLAKQNK++ S KSS +YN + N TDLKED+
Sbjct: 123 DLEGKPASPISRKAKLVGGNGQDLRRTVVSLAKQNKESNSRKSSLEYNPS-NVTDLKEDI 182

Query: 253 ISAPEIVTPSPKRLLPSIKEVHAAAVHYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKG 312
           IS+PEIVTPSPKRLLPS KEV AAAVHYSRTKLNLSLSKV S   QG  R KR+KE++KG
Sbjct: 183 ISSPEIVTPSPKRLLPSTKEVQAAAVHYSRTKLNLSLSKVSSFAGQGGSRTKRNKEIRKG 242

Query: 313 KKQNGDGSSSSRTNRTSRCQQINISAEEDIKALVPQVVSWTPRTPRHRVVRVAIADKKII 372
           KK+ GDGS SS +N TSR  ++N+SAEEDI ALVP+V S   RTPR RV RVAIADKK I
Sbjct: 243 KKKEGDGSLSS-SNSTSRSLEMNVSAEEDITALVPEVGS---RTPRTRVKRVAIADKKNI 302

Query: 373 GRRGLKNQRHPKKCKPDPSINEVVEEKTIYMIEPSTKNETEESAQNSVDTTELSRPQSSS 432
           GR GLK+Q HP KCKPDPS NE VEEKT+YMIEPSTK+ETEE +QNSV TTE S+PQSSS
Sbjct: 303 GRNGLKSQTHPIKCKPDPSNNEDVEEKTLYMIEPSTKDETEEISQNSVHTTESSQPQSSS 362

Query: 433 ATDNSLKHEQEADGTPVVPPLSVKNN-VRRARNETKSSKILSTSSPVSGGVKGLRPKRFG 492
            TDN+LKHEQEA    +VPP+SVK N V+RARN T S+KIL TS   S   KG+RPKRFG
Sbjct: 363 TTDNNLKHEQEAAANSIVPPMSVKKNVVKRARNGT-SAKILCTSPTASKVFKGIRPKRFG 422

Query: 493 MTPRSETRSAPSSPSSSRGPSEPVHGKRGGTTSGKDVKKGEKSKVEHRIKIQRKALTDTE 552
           M  RSETRSAPSSP SSR  SEP+H +  G+TSG DVKK E SKV+HR+K +   LTD+E
Sbjct: 423 MVQRSETRSAPSSPLSSRFQSEPIHVEHRGSTSGNDVKKSENSKVDHRLKTKGMTLTDSE 482

Query: 553 NEDCQPRKLKFRKGKTVELQTETITPRRLKFRRVRLLGETHSLKTDSRKRNIKSKEANQN 612
           N DCQ RKLKFRKGKTVELQ ET +PRRLKFR VRLLGET S K DSRKRNI  K+ NQN
Sbjct: 483 NGDCQSRKLKFRKGKTVELQPETSSPRRLKFRHVRLLGETQSPKGDSRKRNIMGKDGNQN 542

Query: 613 GDEVKEAENSSLRQQDQEIKKKKSFRRKETIDYKLVSSRIKSERVVLKHQDSKGKKEIQN 672
           G   KE ENSSLRQQD+++KKK+SFR     D KL+SSR KSERVVL+HQDSKGKKEI N
Sbjct: 543 G---KEGENSSLRQQDKDLKKKRSFR-----DGKLISSRFKSERVVLRHQDSKGKKEILN 602

Query: 673 LFNNVIEETASKLAQTRKSKVKALVGAFETVISLQDTRPAAT 694
           LFNNVIEETASKLA+TRKSKVKALVGAFETVISLQDT+PAAT
Sbjct: 603 LFNNVIEETASKLAKTRKSKVKALVGAFETVISLQDTKPAAT 630

BLAST of Spg036278 vs. ExPASy TrEMBL
Match: A0A6J1KGB0 (uncharacterized protein LOC111492963 OS=Cucurbita maxima OX=3661 GN=LOC111492963 PE=4 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 3.1e-197
Identity = 429/640 (67.03%), Postives = 474/640 (74.06%), Query Frame = 0

Query: 72  ENTDIDMPLAVEVSEDEGSEDKSFDIPVVAVTEISEPEDIIEESIDI------------- 131
           ENTDI  PL VEVS DEG + +SFDIP     E++EP  IIE S DI             
Sbjct: 4   ENTDI--PLVVEVSGDEGRQHESFDIP--TNMEVNEPNAIIEGSTDISLALEASADEGSQ 63

Query: 132 ---IDIPASTEANEPESSNVEVIVDISTPKLGRKIPSRYLHPHSSSCHDFCKYGVKNALE 191
               DIP + E NEPES NV VIV+I+TPK  R  P RYL P + SCHDFCKYG K+ +E
Sbjct: 64  DEKFDIPTTMEVNEPESCNVGVIVNINTPKFRRIFP-RYLSPRTGSCHDFCKYGTKHPIE 123

Query: 192 GKPASPVLRKDKSVGGNGQDLRRIIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDVISA 251
             PAS VLRK KSVG + +DLRRI V+LAK N D +S K S DY+  IN TDLKEDV S+
Sbjct: 124 RNPASAVLRKGKSVGRDVRDLRRIRVTLAKPNNDTVSPKFSKDYD-GINITDLKEDVTSS 183

Query: 252 PEIVTPSPKRLLPSIKEVHAAAVHYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKGKKQ 311
           PEI+ PSPK+ LP IKEV AAAV YSRTKLNLSLSK  S   Q + R  R+KEV++ KKQ
Sbjct: 184 PEIINPSPKKRLPPIKEVEAAAVQYSRTKLNLSLSKASSSAGQCTSRTNRNKEVRRSKKQ 243

Query: 312 NGDGSSSSRTNRTSRCQQINISAEEDIKALVPQVVSWTPRTPRHRVVRVAIADKKIIGRR 371
           +G GSSSS T+ TSRCQ+I IS   D KALVP  VSW   TPR+RV RVAI DKKIIGR 
Sbjct: 244 DGYGSSSSSTDSTSRCQEIKISTVGDRKALVPHAVSW---TPRNRVKRVAIVDKKIIGRH 303

Query: 372 GLKNQRHPKKCKPDPSINEVVEEKTIYMIEPSTKNETEESAQNSVDTTELSRPQSSSATD 431
            LKNQ   KK KPDPS +EVVEEKT+YMIEPSTK ETE  AQNSV  TELS PQSSSATD
Sbjct: 304 SLKNQSRLKKIKPDPSNSEVVEEKTLYMIEPSTKKETEGPAQNSVHFTELSHPQSSSATD 363

Query: 432 NSLKHEQEADGTPVVPPLSVKNNVRRARNETKSSKILSTSSPVSGGVKGLRPKRFGMTPR 491
           NS KH+QEADGT + P L V+ N RR RN T SSK LSTS  V  G KGLRPKRF M   
Sbjct: 364 NSFKHKQEADGTIITPHLLVEENTRRTRNGT-SSKSLSTSPTVFKGFKGLRPKRFDMIQM 423

Query: 492 SETRSAPSSPSSSRGPSEPVHGKRGGTTSGKDVKKGEKSKVEHRIKIQRKALTDTENEDC 551
           SETRSAPSSPSSSR  SEPVH              GEKSKVEH+IK +RK LTD+EN DC
Sbjct: 424 SETRSAPSSPSSSRCSSEPVH--------------GEKSKVEHKIKARRKTLTDSENGDC 483

Query: 552 QPRKLKFRKGKTVELQTETITPRRLKFRRVRLLGETHSLKTDSRKRNIKSKEANQNGDEV 611
           Q RKL FRKG+ VELQTE ITPRRL F+RVR L ET S K+DSRKR I+ KEANQNGDEV
Sbjct: 484 QSRKLHFRKGRMVELQTEPITPRRLIFKRVRSLSETQSPKSDSRKRIIQRKEANQNGDEV 543

Query: 612 KEAENSSLRQQDQEIKKKKSFRRKETIDYKLVSSRIKSERVVLKHQDSKGKKEIQNLFNN 671
           KE ENSSLRQQDQE K+KKSFRR+ETID KLVSSR+KSER+VLKHQDS  K EIQ L NN
Sbjct: 544 KETENSSLRQQDQEFKRKKSFRRQETIDGKLVSSRLKSERIVLKHQDSNEKIEIQKLLNN 603

Query: 672 VIEETASKLAQTRKSKVKALVGAFETVISLQDTRPAATVV 696
           VIEETA+KLA+TRKSKVKALVGAFETVISLQD +PAATVV
Sbjct: 604 VIEETATKLAKTRKSKVKALVGAFETVISLQDAKPAATVV 619

BLAST of Spg036278 vs. ExPASy TrEMBL
Match: A0A6J1C636 (uncharacterized protein LOC111008620 OS=Momordica charantia OX=3673 GN=LOC111008620 PE=4 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 5.9e-196
Identity = 443/739 (59.95%), Postives = 504/739 (68.20%), Query Frame = 0

Query: 71  EENTDIDMPLAVEVSEDEGSEDKSFDIPVVAVTEISEPEDIIEESIDIIDIPASTEANEP 130
           EENT I  P+AVE S DEGS+D+SFDIPV+AV EISEP+DI EESID  DI A  E NE 
Sbjct: 3   EENTGI--PVAVETSGDEGSQDESFDIPVIAVAEISEPDDISEESID--DILAIPEENES 62

Query: 131 ESSNVEVIVDISTPKLGRKIPSRYLHPHSSSCHDFCKYGVKNALEGKPASPVLRKDKSVG 190
           E S+VEVIVDI  PK+GRKIPSRYL P++ SCHDFCKYG K+ LEGKPAS VL+K  +VG
Sbjct: 63  ERSSVEVIVDIDAPKIGRKIPSRYLLPYAGSCHDFCKYGTKHPLEGKPASLVLKKVITVG 122

Query: 191 GNGQDLRRIIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDVISAPEIVTPSPKRLLPSI 250
           G+G+D RR ++SLAKQNKD I++KSSPDYN A+NNTD KED+I +PEIV  SPKRLLPS 
Sbjct: 123 GDGRDSRRTMISLAKQNKDPINTKSSPDYN-AMNNTDSKEDIIYSPEIVILSPKRLLPSS 182

Query: 251 KEVHAAAVHYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKGKKQNGDGSSSSRTN-RTS 310
           KE+ AAAVHYSRTKLNLS SK  S   QGS   KR+KEVQ+G++ +GDGS SSRTN  TS
Sbjct: 183 KEMQAAAVHYSRTKLNLSHSKRSSFSGQGSSGTKRNKEVQEGQELDGDGSISSRTNSSTS 242

Query: 311 RCQQINISAEEDIKALVPQVVSWTPRTPRHRVVRVAIADKKIIGRRGLKNQRHPKKCKPD 370
           RCQ+I+I  EED+KALVPQ +S   R+PR+   RVA+ADKK+ GRR LKN+ HP K K  
Sbjct: 243 RCQEIDIFEEEDMKALVPQAIS---RSPRNHGKRVAVADKKVTGRRRLKNRSHPMKTKLV 302

Query: 371 PSINEVVEEKTIYMIEPSTKNETEESAQNSVDTTELSRPQSSSATDNSLKHEQEADGTPV 430
              NE VEEKT+YMIEPSTKNE EE AQNSV TT+LSRP+SSSA DN LKH+QEADGT  
Sbjct: 303 HCKNEDVEEKTLYMIEPSTKNEAEELAQNSVHTTDLSRPKSSSAEDNGLKHDQEADGTSK 362

Query: 431 VPPLSVKNNVRRARNETKSSKILSTSSPVSGGVKGLRPKRFGMTPRSETRSAPSSPSSSR 490
           VP  SV+    RARN T SSK L TSS VS G KGLRPKR+GM  RS+ +SAPSS S SR
Sbjct: 363 VPQ-SVEKIASRARNGT-SSKNLFTSSSVSRGSKGLRPKRYGMIQRSKIQSAPSSSSLSR 422

Query: 491 GPSEPVHGKRGGTTSGKDVKKGEKSKVEHRIKIQR------------------------- 550
            P+EPVHG+ GG  SGKDVKK E SKVEH+IK +R                         
Sbjct: 423 YPAEPVHGEHGGRPSGKDVKKSEHSKVEHKIKTRRTTVYDSENGDFQSRKLKPRKGRLVE 482

Query: 551 ------------------------------------------------------------ 610
                                                                       
Sbjct: 483 LQPENNSPRRPKFLSDTENGDSQSRKLKLRKGRLVELQPENNSLRGPKFLSDSENGDSQS 542

Query: 611 -----------------------KALTDTENEDCQPRKLKFRKGKTVELQTETITPRRLK 670
                                  K L+D+EN D Q RKLKFR+G+ VELQ E   PRRLK
Sbjct: 543 RKLKLRKGRLVELQPENNSPRSPKFLSDSENGDVQSRKLKFREGRLVELQPENNAPRRLK 602

Query: 671 FRRVRLLGE--THSLKTDSRKRNIKSKEA----NQNGDEVKEAENSSLRQQDQEIKKKKS 695
           FRR RLLGE  T S K+DSR+RN + KEA    NQNGDE KE E+ S RQQDQE K+KKS
Sbjct: 603 FRRARLLGETQTQSPKSDSRRRNFQGKEANQNGNQNGDEAKETEHGSSRQQDQETKRKKS 662

BLAST of Spg036278 vs. TAIR 10
Match: AT5G15430.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 81.6 bits (200), Expect = 2.7e-15
Identity = 144/588 (24.49%), Postives = 240/588 (40.82%), Query Frame = 0

Query: 141 ISTPKLG-----RKIPSRYLHPHSSSCHDFCKYGVKNALEGKPASPVLRK-DKSVGG--- 200
           IST KL       K+   YL   + SCHD CKYG K+  E KP  P  ++  +S  G   
Sbjct: 21  ISTGKLSFLYTQEKVVPNYLRSPTGSCHDACKYGRKDESEDKPRVPHRKRVSRSFSGAIN 80

Query: 201 -----NGQDLRRIIVSLAKQ---------NKDAISSKSSPDYNKAINNTD-LKEDVISAP 260
                  + L + ++S +++          K  + + SS   +   ++ D   E V+S  
Sbjct: 81  LDSPLRKKALTKPLLSPSRRCDSVGGFDHAKSQVRNFSSGVCDVKKSHADGTNEKVVSVS 140

Query: 261 EI-VTPSPKRLLPSIKEVHAA---AVHYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKG 320
           E  +  S KR     K V+ +   A      K  ++  K+ ++ +   + ++RS    K 
Sbjct: 141 ESRLADSTKRKKKKKKTVYVSRGRAKEIVEQKRRVTALKLKAVAQTAEIALRRS--TVKR 200

Query: 321 KKQNGDGSSSSRTNRTSRCQQINISAEEDIKALVPQVVSWTPRTPRHRVVRVAIADKKII 380
           KK NG   ++ +       ++ ++S++   + L  +  S +   P  +        +K +
Sbjct: 201 KKMNGGSKAAEQKKAVMALRRASMSSKGCSRCLKTKKESNSLSVPLKKT-------RKHV 260

Query: 381 GRRGLKNQRHPKKCKPDPSINEVVEEKTIYMIEPSTKNETEESA--QNSVDTTELSRPQS 440
           G           KCK      ++VEEKT+Y+I+  T +E  ES   Q  V  + +  P+S
Sbjct: 261 G----------DKCK------DLVEEKTLYVIKMETVDEIVESELNQRCVMDSPIDDPKS 320

Query: 441 SSATDN----SLKHEQEADGTPVVPPLSVKNNVRRARNETKSSKILSTSSPVSGGVKGLR 500
             + D       +HE E+               +   +E +  + +S S           
Sbjct: 321 EKSQDKGECIETEHEDESS--------------QEEEDEEEEDENVSVSE---------- 380

Query: 501 PKRFGMTPRSETRSAPSSPSSSRGPSEPVHGKRGGTTSGKDVKKGEKSKVEHRIKIQRKA 560
                   ++ TR   S   S+                                     A
Sbjct: 381 -------DKNTTREGKSKAFSA-----------------------------------ESA 440

Query: 561 LTDTENEDCQPRKLKFRKGKTVELQTETITPRRLKFRRVRLLGETHSLKTDSRKRNIKSK 620
           +T          KL+ R+GK ++  +E  +PR+LKF+R +++    +      +R +K+K
Sbjct: 441 ITG------NAMKLRIRRGKIIDFGSEGNSPRKLKFKRGKIISGADTTSKSGGRRRLKTK 477

Query: 621 EANQNGDEVKEAENSSLRQQDQEIKKKKSFRRKETIDYKLVSSRIKSERVVLKHQDSKGK 680
             N + D+ ++                                  +  RVVLKHQD++ K
Sbjct: 501 GTNLSNDKEQQ----------------------------------RKPRVVLKHQDTEKK 477

Query: 681 KEIQ-NLFNNVIEETASKLAQTRKSKVKALVGAFETVISLQDTRPAAT 694
           +E +  LFN VI+ETA+KL QTRKSKVKALVGAFE+VISLQ+   +AT
Sbjct: 561 RESRVLLFNKVIKETANKLVQTRKSKVKALVGAFESVISLQEKTSSAT 477

BLAST of Spg036278 vs. TAIR 10
Match: AT5G39380.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 76.3 bits (186), Expect = 1.1e-13
Identity = 138/552 (25.00%), Postives = 231/552 (41.85%), Query Frame = 0

Query: 142 STPKLGRKIPSRYLHPHSSSCHDFCKYGVKNALEGKPASPVLRK--DKSVGGN-GQDLRR 201
           S PK  +KIP  YL   + SCHD CKYG +     KP     +K   KS+  N  + L+ 
Sbjct: 35  SKPK-EKKIP-HYLRASTGSCHDLCKYGKRQIPVEKPWRSSTKKIFKKSLDDNLNETLKP 94

Query: 202 IIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDVISAPEIVTPSPKRLLPSIKEVHAAAV 261
               + K+ ++   ++ + D  + I    +K         +      ++ S  E     +
Sbjct: 95  GSSKMKKKVREVEKNEGTDDSFEVIKREVVKYQASGVSSGMRKPEVLIISSCDETPVKQI 154

Query: 262 HYSRTKLNLSLSKVPSIKRQGSLRIKRSKEVQKGKKQNGDGSSSSRTNRTSRCQQINISA 321
              +T L+  L   P +  + S  +   K     K  +   +S  + N            
Sbjct: 155 K-KKTTLSSKLKPSPDLGSRSSENVDALKPKVLKKSYSALTTSKPKVNHE---------- 214

Query: 322 EEDIKALVPQVVSWTPRTPRHRVVRVAIADKKIIGRRGLKNQRHPKKCKPDPSINEVVEE 381
               K +   V+   P+  +    +    D K+  ++G  + R   K  P      +   
Sbjct: 215 ----KVVASPVLK--PKMGKRNDGK--DEDGKV--KKGTVSSRVASKKAPVTPRASLSPR 274

Query: 382 KTIYMIEPSTKNETEESAQNSVDTTELSRPQSSSATDNSLKHEQEADGTPVVPPLSVKNN 441
            ++ +   S+  +++     S  + +  +P+  + TD   +  ++ D  PV      +  
Sbjct: 275 LSVRLAGSSSLRKSQSLKAASSSSRQNQKPRPVNRTD---EFNKQLDDYPV-----EEKT 334

Query: 442 VRRARNETKSSKILSTSSPVSGGVKGLRPKRFGMTPRSETRSAPSSPSSSRGPSEPVHGK 501
           +     ET ++ +        G V+   P      P   T+S P     +   +E     
Sbjct: 335 LHVVEMETTNNVVSENDQNQQGFVEPFLP------PLPPTQSTPKDDECTVSETEEYE-- 394

Query: 502 RGGTTSGKDVKKGEKSKV--EHRIKIQRKALTDTENEDCQPRKLKFRKGKTVELQTETIT 561
               TSG +  + E+ ++   +  K  R A  + ++ D   RKL+FR+G  V+  T    
Sbjct: 395 ---YTSGSNEAESEEEEIGLSNGEKKPRAARKEGDSADEAARKLRFRRGTIVDPDTVGEK 454

Query: 562 PRRLKFRRVRLLGETHSLKTDSRKRNIKSKEANQNGDEVKEAENSSLRQQDQEIKKKKSF 621
            R+LKFRR R LGE                                   + Q+ + ++SF
Sbjct: 455 ARKLKFRRGRGLGE----------------------------------DKAQDAQVRRSF 507

Query: 622 RRKETIDYKLVSSRIKSERVVLKHQDSKGKKEIQNLFNNVIEETASKLAQTRKSKVKALV 681
           +++E I  + V+     E+VVL+HQD + +K+ Q LFNNVIEETASKL + RKSKVKALV
Sbjct: 515 KKREDIREEEVNE--DGEKVVLRHQDVQ-EKDAQGLFNNVIEETASKLVEARKSKVKALV 507

Query: 682 GAFETVISLQDT 689
           GAFETVISLQ++
Sbjct: 575 GAFETVISLQES 507

BLAST of Spg036278 vs. TAIR 10
Match: AT5G07820.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 72.0 bits (175), Expect = 2.1e-12
Identity = 94/313 (30.03%), Postives = 141/313 (45.05%), Query Frame = 0

Query: 397 QNSVDTTELSR-PQSSSATDNSLKHEQEADGT----PVVPPLSVKNN--VRRARNETKSS 456
           + S+D  ++SR  ++ ++ +  LK+ +  + T    PV P  +V+    V  +  E K  
Sbjct: 263 KTSLDAQKVSRISENKNSKEERLKNLKNKEKTNIDEPVRPDDAVEKTLYVVESSVEKKKK 322

Query: 457 KILSTSSPVSGGVKGLRPKRFGMTPRSETRSAPSSPSSSRGPSEPVHGKR----GGTTSG 516
           K+ + S  +S   +    K    T +      PS P     PSE V G        TTS 
Sbjct: 323 KMSTKSVKISETQQSSEKKIIRSTGKKSLSLLPSLPP----PSEVVTGSDPRPIRQTTSR 382

Query: 517 KDVKKGEKS-----------KVEHRIKIQRKALTDTENEDCQPRKLKFRKGKTVELQTET 576
                 EK            K E +I+ +R  L  T       +++ F+KGK +E + E 
Sbjct: 383 SKTSLPEKKQSGSANLVTNPKPESKIRPKRIGLKVTPPPPPTKQQMNFKKGKVLEPKPED 442

Query: 577 ITPRRLKFRRVRLLGETHSLKTDSRKRNIKSKEANQNGDEVKEAENSSLRQQDQEIKKKK 636
            T   +KF+++                                 +   LR  D   KKK 
Sbjct: 443 STTTSIKFKKI-------------------------------VVQEPKLRTSDVNKKKKS 502

Query: 637 SFRRKETIDYKLVSSRIKSERVVLKHQDSKGKKEIQNLFNNVIEETASKLAQTRKSKVKA 688
              ++E +    ++   K E+VVL+H+  + KK++Q LFNNVIEET +KL + RKSKVKA
Sbjct: 503 LKDKREGVG--KINGEGKREKVVLRHRKVEVKKKLQTLFNNVIEETVNKLEEVRKSKVKA 538

BLAST of Spg036278 vs. TAIR 10
Match: AT5G61260.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 68.6 bits (166), Expect = 2.3e-11
Identity = 155/635 (24.41%), Postives = 256/635 (40.31%), Query Frame = 0

Query: 68  MAEEENTDI-DMPLAVEVSEDEGSEDKSFDIPVVAVTEISEPEDII------EESIDIID 127
           MAEE  +++ D+  + + +  + SE+ S +   V V ++   ED+I      +E I    
Sbjct: 1   MAEENTSEVKDIENSSDTNPPKMSEEGSLE--TVRVKDVENVEDVIAVVAKDQEEI---- 60

Query: 128 IPASTEANEPESSNVEVIVDISTPKLGR-KIPSRYLHPHS-SSCHDFCKYGVKNALEGKP 187
           IP   E  +P+   +      ST  + + ++ SRY   H   S HD CK+G K   +   
Sbjct: 61  IPEIIEVPKPQGGTLR---RHSTGTISKPQVQSRYRGNHRVGSTHDLCKHGKKCDQDDAV 120

Query: 188 ASPVLRKDKSVGGNGQDLRRIIVSLAKQNKDAISSKSSPDYNKAINNTDLKEDVISAPEI 247
               + + KSV   G  + ++    + +      S+ SP   K  ++   K D +     
Sbjct: 121 KPWKIARRKSV--EGSVVIKVETPSSTRKSLGSVSRQSPGITKPDSSVSAKRDAL----- 180

Query: 248 VTPSPKRLLPSIKEVHAAAVH-YSRTKLNLSLSK-VPSIKRQGSLRIKRSKEVQKGKKQN 307
                     ++K+   A+V+  S +K    ++K V  +  + + R +++KE + G    
Sbjct: 181 ----------AVKKKPCASVNSESSSKEGSEIAKSVDGLSVKSNDRARKNKETESG---- 240

Query: 308 GDGSSSSRTNRTSRCQQINISAEEDIKALVPQVVSWTPRTPRHRVVRVAIADKKIIGRRG 367
                              +S    +K  VP +     RT +       +   K+   + 
Sbjct: 241 -------------------LSGSAVVKK-VPAL-----RTDKSS-TSTGVGSSKVCAPKN 300

Query: 368 LKNQRHPKKCKPDPSIN-EVVEEKTIYMIEPSTKNETEESAQNSVDTTELSRPQSSSATD 427
           LKN    +K K   +I+ E V+EKT+ ++E S K    E   +S   T  S  +S S T 
Sbjct: 301 LKN---VEKAKTTQTISGEDVKEKTVCVVESSVKGVKSEKQPSSEKKTMKSGNKSLSTTP 360

Query: 428 NSLKHEQEADGTPVVPPLSVKNNVRRARNETKSSKILSTSSPVSGGVKGLRPKRFGMTPR 487
                                   +R  + TK          + G +        G+T +
Sbjct: 361 ------------------------KRGSSPTKQ---------IPGKIS------TGLTKK 420

Query: 488 SETRSAPSSPSSSRGPSEPVHGKRGGTTSGKDVKKGEKSKVEHRIKIQRKALTDTENEDC 547
            ET SA    ++ + P + V  K          K G K  +  ++  ++  + D + ED 
Sbjct: 421 KETGSADVVEANPK-PEKKVRPK----------KTGVKVSLAQQMTFKKGKVLDPKPEDS 471

Query: 548 QPRKLKFRKGKTVELQTETITPRRLKFRRVRLLGETHSLKTDSRKRNIKSKEANQNGDEV 607
            PR +KF+K    EL+T+                      ++ +K+N+K +     G E 
Sbjct: 481 SPRWIKFKKRVVQELKTQ----------------------SEGKKKNLKDRRL---GVET 471

Query: 608 K--EAENSSLRQQDQEIKKKKSFRRKETIDYKLVSSRIKSERVVLKHQDSKGKKEIQNLF 667
           K    E S                              K E+VVL+H+  +GKK++  LF
Sbjct: 541 KTDSCEGS------------------------------KREKVVLRHRKVEGKKKMITLF 471

Query: 668 NNVIEETASKLAQTRKSKVKALVGAFETVISLQDT 689
           NNVIEET +KL + RK KVKAL+GAFETVISLQDT
Sbjct: 601 NNVIEETVNKLTKVRKHKVKALIGAFETVISLQDT 471

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894083.13.7e-22975.24uncharacterized protein LOC120082825 [Benincasa hispida] >XP_038894084.1 unchara... [more]
XP_008446785.13.2e-21271.75PREDICTED: uncharacterized protein LOC103489380 [Cucumis melo] >XP_008446868.1 P... [more]
XP_011650019.13.5e-21169.31uncharacterized protein LOC105434693 [Cucumis sativus] >XP_011650022.1 uncharact... [more]
XP_022998278.16.4e-19767.03uncharacterized protein LOC111492963 [Cucurbita maxima][more]
XP_022137059.11.2e-19559.95uncharacterized protein LOC111008620 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1S3BFX31.5e-21271.75uncharacterized protein LOC103489380 OS=Cucumis melo OX=3656 GN=LOC103489380 PE=... [more]
A0A5D3BIK31.5e-21271.75CaM_binding domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A0A0LST41.7e-21169.31CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G038960... [more]
A0A6J1KGB03.1e-19767.03uncharacterized protein LOC111492963 OS=Cucurbita maxima OX=3661 GN=LOC111492963... [more]
A0A6J1C6365.9e-19659.95uncharacterized protein LOC111008620 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
Match NameE-valueIdentityDescription
AT5G15430.12.7e-1524.49Plant calmodulin-binding protein-related [more]
AT5G39380.11.1e-1325.00Plant calmodulin-binding protein-related [more]
AT5G07820.12.1e-1230.03Plant calmodulin-binding protein-related [more]
AT5G61260.12.3e-1124.41Plant calmodulin-binding protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012417Calmodulin-binding domain, plantSMARTSM01054CaM_binding_2coord: 536..684
e-value: 1.2E-20
score: 84.6
IPR012417Calmodulin-binding domain, plantPFAMPF07839CaM_bindingcoord: 539..614
e-value: 2.4E-4
score: 21.9
coord: 602..684
e-value: 3.2E-25
score: 89.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 573..614
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 500..519
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 384..519
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 385..417
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 476..491
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 276..310
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 295..310
NoneNo IPR availablePANTHERPTHR33349EMB|CAB62594.1coord: 118..693
NoneNo IPR availablePANTHERPTHR33349:SF1EMB|CAB62594.1coord: 118..693

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg036278.1Spg036278.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding