Spg036275 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg036275
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCalcium-binding EF-hand family protein
Locationscaffold5: 48163468 .. 48165682 (-)
RNA-Seq ExpressionSpg036275
SyntenySpg036275
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGGATTCCCAGCCTTTCTTAACATTGCTTTCCTGGAAATTTCTCAAGAGGTGAGCCAAGCACCTTAACCTTAAAATTCTTCCCCCCACCCCCAAAAAAAGGTACAAGTAGCAATTAATAATTTTTTGATGCATTCTATTTAGAATGACTTTCTAAGTGTGTAAACAAGTATTTTTAAGTGAAAAATATATATTTTTAAACAAAGTCATTCTAAACAGACACACAATTATTATCCTCCATTTAATTTCCACTAATTTTTTCAAAAATAATGTAAAAAACTCCAAACACTCGCTAATTTTATGAAAATCAATATAACATAATTTCATTATCCCTCTCATTCAAATATCGTGTCAATTAAGGATATGTTTGGTTTAACTTTTTAAGTGTTTAATTTTGAAAAAATTTTATAGTATTTGACAACCACTAAAAATGACTTATTAAAAAATGAGATCGAGAAATAAATCATTTTGAGATAAACACTTGAAACCAAATTTTTTAAAAAGATTTTTTAAGTATATAATAATTTGTATATAAAAACACATATGAGAAATGTAAGCAGGCATATGAGTGTTTAAATTTGAAATAATTTTTATACAAAATGTTTAAAAGAAAATTTATCCTTGAAAAACACTCCAAACTAAAAGTTATTCCAAACGGACCCTTAAGACCTCTCGCTCTTGTTGGCATATTTCTTTTTTTTTTAACAATATGGGAAATGGGGATTCGAACCACAGACCTCATGGTTATCGGTACATCTTTATGCTAGTTGAGCTATGCTCTTGTTGGCATATTTCCTATTATTCAAGCATCACATGTCTAACTTTTTCATTCATCTTGATTCACATTGTTGATTGTAAAGGGGAGAGGTCCCACTCTAGTACCTTTTTAACTCTCCAAGCCCAAAATTCAACTGCAAGGAAACATCGGAATAATCGAGTACAAAATCCAAAATCCAAAATTCAAATCTCGATTCGAATCTCGAACTCGAGACGTTACAATTTTAAATCGAAAAAAAAATAATAAATAAATTCAAAATCAAAAGTTTCACTTACTCCATTCATCAATTATGACAAAGAGGGAAAAACAAAACATAAAATTGACTTGGACAACCCTAGTCTATTTCTAAACCTAAAAGTTAAAACTTACAATGCAAGATCCATTAAAAACCCTTATAAAATTTAAAGAAGACAGAGAGTCTCCCTTAGCCGCGGCAGAGACGGGTCGTCCAAGGCTCAAGAAGATGATACTGAAATTTCTCAGAACTTCTCAACTTCATCAACATTCATTCCTTCTGAAAAAGCTTTGCAAAGAAAGACAGAAGTAAAGTCATTGCGTTCACTTTATCGACGTTCTAATTTCCTCGAAAGTTTCACTGTGACTCTGAACACACGCAACAAAATGCGGTGTGGATTCGAGCGCAATAACGCTTGTACGTTTTCAGAAGTTTAATCAGCAAGACTTGACTCCAACTTCAGCGCCATATAAATTGGTAACCCATGATAATTCTTCTCTTGTCTACTGCTGACTGCTCGATTAGCTTTGTAACACTCATATATATTTGCTATTTGTTCACCCTTTAATTTTTTCGAAAATGGCGCCAATTGATGATAACCCCATTTCAGTTTTTGAGATTTTGGTTCTGATTTTTCTCAATTTCGTACTTGAACGCTTCTTTTCAGGTCTCTTGTACATTTTCATGCTCTGTGCTGCGTTCCTTTTTTATCTCTGGAATTCCTGGGTTGCCCACCGCAACATCATCAATCGCCTTGGTGCTGCCGAAGCGACATGCACACCAAGTGTTCGACGAAATGCCCATGAGGGGCATTACTACACAGAACTTCTGGACATGCCCGTTAATGACACACGGGAAGTGAAACTTTCCATTGACGATGTGAAAACTATGATGGAGGCACTTGATCGTCAGCTTAATAACACTGAAACTGATAATCTTGGGTTGAATGCTGAGATTGAGAGGCTGTTTGATGAAGAATTGAGTTTAGGGGAAGTTGAGGAAGCTTTTGATTTGTTTGATGAGAATGGTGATGGGTTTATTGATGCAGAGGACTTGAAGAACGTGTTGTGTGCCATGGGATTTACAGTGGCTTCAGAAGTTGATCAATGCAGGAGAATGATAAGTCCATTTGATAATAATGGAGATGGGCGCTTAGATTTTGAAGAATTTGCCAAGCTTGTTGAACAAAGCTTTGGTTGA

mRNA sequence

ATGTGGGATTCCCAGCCTTTCTTAACATTGCTTTCCTGGAAATTTCTCAAGAGAAGACAGAGAGTCTCCCTTAGCCGCGGCAGAGACGGGTCGTCCAAGGCTCAAGAAGATGATACTGAAATTTCTCAGAACTTCTCAACTTCATCAACATTCATTCCTTCTGAAAAAGCTTTGCAAAGAAAGACAGAAGTAAAGTCATTGCGTTCACTTTATCGACGTTCTAATTTCCTCGAAAGTTTCACTGTGACTCTGAACACACGCAACAAAATGCGGTGTGGATTCGAGCGCAATAACGCTTGTCTCTTGTACATTTTCATGCTCTGTGCTGCGTTCCTTTTTTATCTCTGGAATTCCTGGGTTGCCCACCGCAACATCATCAATCGCCTTGGTGCTGCCGAAGCGACATGCACACCAAGTGTTCGACGAAATGCCCATGAGGGGCATTACTACACAGAACTTCTGGACATGCCCGTTAATGACACACGGGAAGTGAAACTTTCCATTGACGATGTGAAAACTATGATGGAGGCACTTGATCGTCAGCTTAATAACACTGAAACTGATAATCTTGGGTTGAATGCTGAGATTGAGAGGCTGTTTGATGAAGAATTGAGTTTAGGGGAAGTTGAGGAAGCTTTTGATTTGTTTGATGAGAATGGTGATGGGTTTATTGATGCAGAGGACTTGAAGAACGTGTTGTGTGCCATGGGATTTACAGTGGCTTCAGAAGTTGATCAATGCAGGAGAATGATAAGTCCATTTGATAATAATGGAGATGGGCGCTTAGATTTTGAAGAATTTGCCAAGCTTGTTGAACAAAGCTTTGGTTGA

Coding sequence (CDS)

ATGTGGGATTCCCAGCCTTTCTTAACATTGCTTTCCTGGAAATTTCTCAAGAGAAGACAGAGAGTCTCCCTTAGCCGCGGCAGAGACGGGTCGTCCAAGGCTCAAGAAGATGATACTGAAATTTCTCAGAACTTCTCAACTTCATCAACATTCATTCCTTCTGAAAAAGCTTTGCAAAGAAAGACAGAAGTAAAGTCATTGCGTTCACTTTATCGACGTTCTAATTTCCTCGAAAGTTTCACTGTGACTCTGAACACACGCAACAAAATGCGGTGTGGATTCGAGCGCAATAACGCTTGTCTCTTGTACATTTTCATGCTCTGTGCTGCGTTCCTTTTTTATCTCTGGAATTCCTGGGTTGCCCACCGCAACATCATCAATCGCCTTGGTGCTGCCGAAGCGACATGCACACCAAGTGTTCGACGAAATGCCCATGAGGGGCATTACTACACAGAACTTCTGGACATGCCCGTTAATGACACACGGGAAGTGAAACTTTCCATTGACGATGTGAAAACTATGATGGAGGCACTTGATCGTCAGCTTAATAACACTGAAACTGATAATCTTGGGTTGAATGCTGAGATTGAGAGGCTGTTTGATGAAGAATTGAGTTTAGGGGAAGTTGAGGAAGCTTTTGATTTGTTTGATGAGAATGGTGATGGGTTTATTGATGCAGAGGACTTGAAGAACGTGTTGTGTGCCATGGGATTTACAGTGGCTTCAGAAGTTGATCAATGCAGGAGAATGATAAGTCCATTTGATAATAATGGAGATGGGCGCTTAGATTTTGAAGAATTTGCCAAGCTTGTTGAACAAAGCTTTGGTTGA

Protein sequence

MWDSQPFLTLLSWKFLKRRQRVSLSRGRDGSSKAQEDDTEISQNFSTSSTFIPSEKALQRKTEVKSLRSLYRRSNFLESFTVTLNTRNKMRCGFERNNACLLYIFMLCAAFLFYLWNSWVAHRNIINRLGAAEATCTPSVRRNAHEGHYYTELLDMPVNDTREVKLSIDDVKTMMEALDRQLNNTETDNLGLNAEIERLFDEELSLGEVEEAFDLFDENGDGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSFG
Homology
[Close]
BLAST of Spg036275 vs. NCBI nr
Match: XP_038895011.1 (probable calcium-binding protein CML45 [Benincasa hispida])

HSP 1 Score: 284.6 bits (727), Expect = 8.8e-73
Identity = 139/175 (79.43%), Postives = 156/175 (89.14%), Query Frame = 0 

Query: 101 LLYIFMLCAAFLFYLWNSWVAHRNIINRLGAAEATCTPSVRRNAHEGHYYTELLDMPVND 160
           LLY+F+LCAAFLFYL NSW AHRNII RL AA+ATCTPSVRRNAHEGH YT+++ MPVND
Sbjct: 35  LLYVFILCAAFLFYLCNSWAAHRNIIKRLEAADATCTPSVRRNAHEGHCYTDIVVMPVND 94

Query: 161 TREVKLSIDDVKTMMEALDRQLNNTETDNLGLNAEIERLFDEELSLGEVEEAFDLFDENG 220
           T+EVKL+I+DVKTMME LD + NN E++NL LNAEI  LF+EELSLGEV+EAFDLFD+NG
Sbjct: 95  TKEVKLTIEDVKTMMETLDHEHNNAESNNLELNAEIVGLFEEELSLGEVKEAFDLFDDNG 154

Query: 221 DGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSF 276
           DGFID EDLK VLC MGF VA EVDQCRRMIS FD+NGDGR+DFEEFAKLVEQSF
Sbjct: 155 DGFIDVEDLKKVLCGMGFAVALEVDQCRRMISGFDSNGDGRIDFEEFAKLVEQSF 209

[Close]
BLAST of Spg036275 vs. NCBI nr
Match: KAG7020054.1 (putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 270.0 bits (689), Expect = 2.2e-68
Identity = 138/175 (78.86%), Postives = 151/175 (86.29%), Query Frame = 0 

Query: 102 LYIFMLCAAFLFYLWNSWVAHRNIINRLGAAE-ATCTPSVRRNAHEGHYYTELLDMPVND 161
           LY+FMLCAAF FYLWNSWVAHRNII R  AA+ ATCTPS RRNAH GH YT ++ MPVND
Sbjct: 36  LYVFMLCAAFHFYLWNSWVAHRNIIKRFEAADGATCTPSARRNAHVGHCYTGIVGMPVND 95

Query: 162 TREVKLSIDDVKTMMEALDRQLNNTETDNLGLNAEIERLFDEELSLGEVEEAFDLFDENG 221
             EVKL+I+DVKTMMEAL    NN ET+NL L AEIE +F+EELS+GEV+EAFDLFDENG
Sbjct: 96  AHEVKLTIEDVKTMMEALH---NNMETNNLELKAEIEGVFEEELSVGEVKEAFDLFDENG 155

Query: 222 DGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSF 276
           DGFIDAEDLK VL AMGFTVASEVD+CRRMIS +D NGDGRLDFEEFAKLVEQSF
Sbjct: 156 DGFIDAEDLKKVLDAMGFTVASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSF 207

[Close]
BLAST of Spg036275 vs. NCBI nr
Match: KAG6584466.1 (putative calcium-binding protein CML46, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 269.6 bits (688), Expect = 2.9e-68
Identity = 139/182 (76.37%), Postives = 153/182 (84.07%), Query Frame = 0 

Query: 95  ERNNACLLYIFMLCAAFLFYLWNSWVAHRNIINRLGAAE-ATCTPSVRRNAHEGHYYTEL 154
           E+ N   LY+FMLCAAF FYLWNSWVAHRNII R  AA+ ATCTPS RRNAH GH YT +
Sbjct: 66  EKFNQGPLYVFMLCAAFHFYLWNSWVAHRNIIKRFEAADGATCTPSARRNAHVGHCYTGI 125

Query: 155 LDMPVNDTREVKLSIDDVKTMMEALDRQLNNTETDNLGLNAEIERLFDEELSLGEVEEAF 214
           + MPVND  EVKL+I+DVKTMMEAL    NN ET+NL L AEIE +F+EELS+GEV+EAF
Sbjct: 126 VGMPVNDAHEVKLTIEDVKTMMEALH---NNMETNNLELKAEIEGVFEEELSVGEVKEAF 185

Query: 215 DLFDENGDGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQ 274
           DLFDENGDGFIDAEDLK VL AMGFTVASE D+CRRMIS +D NGDGRLDFEEFAKLVEQ
Sbjct: 186 DLFDENGDGFIDAEDLKKVLDAMGFTVASEFDECRRMISGYDKNGDGRLDFEEFAKLVEQ 244

Query: 275 SF 276
           SF
Sbjct: 246 SF 244

[Close]
BLAST of Spg036275 vs. NCBI nr
Match: XP_023001351.1 (probable calcium-binding protein CML47 [Cucurbita maxima])

HSP 1 Score: 268.9 bits (686), Expect = 5.0e-68
Identity = 137/175 (78.29%), Postives = 151/175 (86.29%), Query Frame = 0 

Query: 102 LYIFMLCAAFLFYLWNSWVAHRNIINRLGAA-EATCTPSVRRNAHEGHYYTELLDMPVND 161
           LY+F+LCAAF FYLWNSWVAHRNII R  AA +ATCTPS RRNAH GH YT ++ MPVND
Sbjct: 47  LYVFILCAAFHFYLWNSWVAHRNIIKRFEAADDATCTPSARRNAHMGHCYTGIVGMPVND 106

Query: 162 TREVKLSIDDVKTMMEALDRQLNNTETDNLGLNAEIERLFDEELSLGEVEEAFDLFDENG 221
             EVKL+I+DVKTMM+AL    NN ET+NL L AEIE +F+EELS+GEVEEAFDLFDENG
Sbjct: 107 AHEVKLTIEDVKTMMDALH---NNMETNNLELKAEIEGVFEEELSVGEVEEAFDLFDENG 166

Query: 222 DGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSF 276
           DGFIDAEDLK VL AMGFTVASEVD+CRRMIS +D NGDGRLDFEEFAKLVEQSF
Sbjct: 167 DGFIDAEDLKKVLDAMGFTVASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSF 218

[Close]
BLAST of Spg036275 vs. NCBI nr
Match: XP_023519469.1 (probable calcium-binding protein CML47 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 268.9 bits (686), Expect = 5.0e-68
Identity = 138/175 (78.86%), Postives = 150/175 (85.71%), Query Frame = 0 

Query: 102 LYIFMLCAAFLFYLWNSWVAHRNIINRLGAA-EATCTPSVRRNAHEGHYYTELLDMPVND 161
           LY+F+LCAAF FYLWNSWVAHRNII R  AA +ATCTPS RRNAH GH YT ++ MPVND
Sbjct: 47  LYVFILCAAFHFYLWNSWVAHRNIIKRFEAADDATCTPSARRNAHVGHCYTGIVGMPVND 106

Query: 162 TREVKLSIDDVKTMMEALDRQLNNTETDNLGLNAEIERLFDEELSLGEVEEAFDLFDENG 221
             EVKL+IDDVKTMMEAL    NN ET NL L AEIE +F+EELS+GEV+EAFDLFDENG
Sbjct: 107 AYEVKLTIDDVKTMMEALH---NNMETSNLELKAEIEGVFEEELSVGEVKEAFDLFDENG 166

Query: 222 DGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSF 276
           DGFIDAEDLK VL AMGFTVASEVD+CRRMIS +D NGDGRLDFEEFAKLVEQSF
Sbjct: 167 DGFIDAEDLKKVLDAMGFTVASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSF 218

[Close]
BLAST of Spg036275 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.9e-17
Identity = 49/118 (41.53%), Postives = 72/118 (61.02%), Query Frame = 0 

Query: 159 NDTREVKLSIDDVKTMMEALDRQLNNTETDNLGLNAEIERLFDE-ELSLGEVEEAFDLFD 218
           +D  ++ +S ++ + +M +L    N+ +        E+  LF+E E SL EV++AFD+FD
Sbjct: 76  DDDDDIDISREEAEMVMRSLGLFYNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVFD 135

Query: 219 ENGDGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSF 276
           EN DGFIDA +L+ VL  +GF   S +D C  MI   D N DG++DF EF K +E SF
Sbjct: 136 ENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETSF 193

[Close]
BLAST of Spg036275 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 7.1e-17
Identity = 54/133 (40.60%), Postives = 78/133 (58.65%), Query Frame = 0 

Query: 149 YYTELLDMPVNDTREVKLSIDDVKTMMEALDRQLNNTETDNLGL-----NAEIERLFDE- 208
           + T +      D  +  L  +DV  +M++L     +T+ +N GL     + E+  LF+E 
Sbjct: 74  FQTSIKHEEYRDDDDDGLCREDVGMVMKSLGL---STDQENEGLQKQYSSKEVSNLFEEK 133

Query: 209 ELSLGEVEEAFDLFDENGDGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRL 268
           E SL EV++AFD+FDEN DGFID  DL+ VL  +G    S ++ CRRMI  FD + DGR+
Sbjct: 134 EPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRI 193

Query: 269 DFEEFAKLVEQSF 276
           DF  F K +E +F
Sbjct: 194 DFYGFVKFMENNF 203

[Close]
BLAST of Spg036275 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.5e-14
Identity = 46/115 (40.00%), Postives = 69/115 (60.00%), Query Frame = 0 

Query: 163 EVKLSIDDVKTMMEALDRQLNNTETDNLGLNAEIERLFDEELSLGE--VEEAFDLFDENG 222
           EVK  IDD + + E L             +    E L ++   +G+  V+EAF LFDEN 
Sbjct: 82  EVKAIIDDSEALYECL-------------IEEGEEYLLEKNEMMGKEIVKEAFRLFDENQ 141

Query: 223 DGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSF 276
           DGFID  +LK+VL  +G+   +++ +CR+M+  +D N DG++DF EF KL+E+SF
Sbjct: 142 DGFIDENELKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKIDFYEFVKLIEKSF 182

[Close]
BLAST of Spg036275 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.3e-12
Identity = 31/63 (49.21%), Postives = 47/63 (74.60%), Query Frame = 0 

Query: 209 VEEAFDLFDENGDGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFA 268
           + EAF++FD+NGDGFI  ++L++VL ++G       D CRRMIS  D +GDGR+DF+EF 
Sbjct: 100 MREAFNVFDQNGDGFITVDELRSVLSSLGLKHGRTADDCRRMISMVDADGDGRVDFKEFK 159

Query: 269 KLV 272
           +++
Sbjct: 160 QMM 162

[Close]
BLAST of Spg036275 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.6e-11
Identity = 31/71 (43.66%), Postives = 47/71 (66.20%), Query Frame = 0 

Query: 201 DEELSLGEVEEAFDLFDENGDGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDG 260
           +EE   G++ EAF +FD NGDG+I  ++L  VL ++G       ++CRRMI   D +GDG
Sbjct: 171 EEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLKQGRTAEECRRMIGQVDRDGDG 230

Query: 261 RLDFEEFAKLV 272
           R+DF EF +++
Sbjct: 231 RVDFHEFLQMM 241

[Close]
BLAST of Spg036275 vs. ExPASy TrEMBL
Match: A0A6J1KID5 (probable calcium-binding protein CML47 OS=Cucurbita maxima OX=3661 GN=LOC111495509 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 2.4e-68
Identity = 137/175 (78.29%), Postives = 151/175 (86.29%), Query Frame = 0 

Query: 102 LYIFMLCAAFLFYLWNSWVAHRNIINRLGAA-EATCTPSVRRNAHEGHYYTELLDMPVND 161
           LY+F+LCAAF FYLWNSWVAHRNII R  AA +ATCTPS RRNAH GH YT ++ MPVND
Sbjct: 47  LYVFILCAAFHFYLWNSWVAHRNIIKRFEAADDATCTPSARRNAHMGHCYTGIVGMPVND 106

Query: 162 TREVKLSIDDVKTMMEALDRQLNNTETDNLGLNAEIERLFDEELSLGEVEEAFDLFDENG 221
             EVKL+I+DVKTMM+AL    NN ET+NL L AEIE +F+EELS+GEVEEAFDLFDENG
Sbjct: 107 AHEVKLTIEDVKTMMDALH---NNMETNNLELKAEIEGVFEEELSVGEVEEAFDLFDENG 166

Query: 222 DGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSF 276
           DGFIDAEDLK VL AMGFTVASEVD+CRRMIS +D NGDGRLDFEEFAKLVEQSF
Sbjct: 167 DGFIDAEDLKKVLDAMGFTVASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSF 218

[Close]
BLAST of Spg036275 vs. ExPASy TrEMBL
Match: A0A5A7T3X0 (Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00940 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 9.2e-68
Identity = 131/176 (74.43%), Postives = 151/176 (85.80%), Query Frame = 0 

Query: 101 LLYIFMLCAAFLFYLWNSWVAHRNIINRLGAAEATCTPSVRRNAHEGHYYTELLDMPVND 160
           L YIF+L AAFLFYLWNSW A RNII RL AA+ TCTPSVR+N HE H  T+++  PVND
Sbjct: 35  LFYIFILYAAFLFYLWNSWAAQRNIIRRLEAADVTCTPSVRQNDHEEHCCTKIVGTPVND 94

Query: 161 TREVKLSIDDVKTMMEALDRQLNNT-ETDNLGLNAEIERLFDEELSLGEVEEAFDLFDEN 220
            REVKLSI+DVKTMME LD + NN  ++DNLGL+AE+E +F+EELSLGEV+EAFDLFDEN
Sbjct: 95  KREVKLSIEDVKTMMETLDHEHNNNRDSDNLGLSAELEEMFEEELSLGEVQEAFDLFDEN 154

Query: 221 GDGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSF 276
           GDGFIDAEDL+ VLC +GF VAS++DQC+ MIS FDNNGDGRLDFEEFAKLVEQSF
Sbjct: 155 GDGFIDAEDLEKVLCGLGFAVASDIDQCKTMISGFDNNGDGRLDFEEFAKLVEQSF 210

[Close]
BLAST of Spg036275 vs. ExPASy TrEMBL
Match: A0A1S3BEM1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103488781 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 6.0e-67
Identity = 130/176 (73.86%), Postives = 150/176 (85.23%), Query Frame = 0 

Query: 101 LLYIFMLCAAFLFYLWNSWVAHRNIINRLGAAEATCTPSVRRNAHEGHYYTELLDMPVND 160
           L YIF+L AAFLFYLWNSW A RNII RL AA+ TCTPSVR+N HE H  T+++  PVND
Sbjct: 35  LFYIFILYAAFLFYLWNSWAAQRNIIRRLEAADVTCTPSVRQNDHEEHCCTKIVGTPVND 94

Query: 161 TREVKLSIDDVKTMMEALDRQLNNT-ETDNLGLNAEIERLFDEELSLGEVEEAFDLFDEN 220
            REVKLSI+DVKTMME LD + NN  ++DNLGL+AE+E +F+EELSLGEV+EAFDLFDEN
Sbjct: 95  KREVKLSIEDVKTMMETLDHEHNNNRDSDNLGLSAELEEMFEEELSLGEVQEAFDLFDEN 154

Query: 221 GDGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSF 276
            DGFIDAEDL+ VLC +GF VAS++DQC+ MIS FDNNGDGRLDFEEFAKLVEQSF
Sbjct: 155 DDGFIDAEDLEKVLCGLGFAVASDIDQCKTMISGFDNNGDGRLDFEEFAKLVEQSF 210

[Close]
BLAST of Spg036275 vs. ExPASy TrEMBL
Match: A0A6J1ECX5 (probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC111431403 PE=4 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 3.0e-66
Identity = 134/171 (78.36%), Postives = 147/171 (85.96%), Query Frame = 0 

Query: 106 MLCAAFLFYLWNSWVAHRNIINRLGAAE-ATCTPSVRRNAHEGHYYTELLDMPVNDTREV 165
           MLCAAF FYLWNSWVAHRNII R  A++ ATCTPS RRNAH GH YT ++ MPVND  EV
Sbjct: 1   MLCAAFHFYLWNSWVAHRNIIKRFEASDGATCTPSARRNAHVGHCYTGIVGMPVNDAHEV 60

Query: 166 KLSIDDVKTMMEALDRQLNNTETDNLGLNAEIERLFDEELSLGEVEEAFDLFDENGDGFI 225
           KL+I+DVKTMMEAL    NN ET+NL L AEIE +F+EELS+GEV+EAFDLFDENGDGFI
Sbjct: 61  KLTIEDVKTMMEALH---NNMETNNLELKAEIEGVFEEELSVGEVKEAFDLFDENGDGFI 120

Query: 226 DAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSF 276
           DAEDLK VL AMGFTVASEVD+CRRMIS +D NGDGRLDFEEFAKLVEQSF
Sbjct: 121 DAEDLKKVLDAMGFTVASEVDECRRMISGYDKNGDGRLDFEEFAKLVEQSF 168

[Close]
BLAST of Spg036275 vs. ExPASy TrEMBL
Match: A0A6J1C8B5 (uncharacterized protein LOC111008903 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008903 PE=4 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 2.1e-56
Identity = 114/152 (75.00%), Postives = 136/152 (89.47%), Query Frame = 0 

Query: 101 LLYIFMLCAAFLFYLWNSWVAHRNIINRLGAAE-ATCTPSVRRNAHEGHYYTELLDMPVN 160
           LLYIF+LCAAFLFYLW SWVAHRN + RL AA+ ATC  SVR+NA+E HY TE++D+P+N
Sbjct: 35  LLYIFILCAAFLFYLWKSWVAHRNFVQRLDAADSATCMRSVRQNAYEEHYPTEIVDVPIN 94

Query: 161 DTREVKLSIDDVKTMMEALDRQLN-NTETDNLGLNAEIERLFDEELSLGEVEEAFDLFDE 220
           DTREVKLSI+DVKTMMEALDR+ + N E +NLGL+AEIE LF+EE+SLGEV+EAFDLFDE
Sbjct: 95  DTREVKLSIEDVKTMMEALDREHDCNNENNNLGLSAEIEGLFEEEVSLGEVKEAFDLFDE 154

Query: 221 NGDGFIDAEDLKNVLCAMGFTVASEVDQCRRM 251
           NGDGFIDAE+LK VLCAMGFTVAS +D+C++M
Sbjct: 155 NGDGFIDAEELKKVLCAMGFTVASGIDECKKM 186

[Close]
BLAST of Spg036275 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 91.3 bits (225), Expect = 1.3e-18
Identity = 49/118 (41.53%), Postives = 72/118 (61.02%), Query Frame = 0 

Query: 159 NDTREVKLSIDDVKTMMEALDRQLNNTETDNLGLNAEIERLFDE-ELSLGEVEEAFDLFD 218
           +D  ++ +S ++ + +M +L    N+ +        E+  LF+E E SL EV++AFD+FD
Sbjct: 76  DDDDDIDISREEAEMVMRSLGLFYNDDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVFD 135

Query: 219 ENGDGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSF 276
           EN DGFIDA +L+ VL  +GF   S +D C  MI   D N DG++DF EF K +E SF
Sbjct: 136 ENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETSF 193

[Close]
BLAST of Spg036275 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 89.4 bits (220), Expect = 5.1e-18
Identity = 54/133 (40.60%), Postives = 78/133 (58.65%), Query Frame = 0 

Query: 149 YYTELLDMPVNDTREVKLSIDDVKTMMEALDRQLNNTETDNLGL-----NAEIERLFDE- 208
           + T +      D  +  L  +DV  +M++L     +T+ +N GL     + E+  LF+E 
Sbjct: 74  FQTSIKHEEYRDDDDDGLCREDVGMVMKSLGL---STDQENEGLQKQYSSKEVSNLFEEK 133

Query: 209 ELSLGEVEEAFDLFDENGDGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRL 268
           E SL EV++AFD+FDEN DGFID  DL+ VL  +G    S ++ CRRMI  FD + DGR+
Sbjct: 134 EPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRI 193

Query: 269 DFEEFAKLVEQSF 276
           DF  F K +E +F
Sbjct: 194 DFYGFVKFMENNF 203

[Close]
BLAST of Spg036275 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.8e-15
Identity = 46/115 (40.00%), Postives = 69/115 (60.00%), Query Frame = 0 

Query: 163 EVKLSIDDVKTMMEALDRQLNNTETDNLGLNAEIERLFDEELSLGE--VEEAFDLFDENG 222
           EVK  IDD + + E L             +    E L ++   +G+  V+EAF LFDEN 
Sbjct: 82  EVKAIIDDSEALYECL-------------IEEGEEYLLEKNEMMGKEIVKEAFRLFDENQ 141

Query: 223 DGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEFAKLVEQSF 276
           DGFID  +LK+VL  +G+   +++ +CR+M+  +D N DG++DF EF KL+E+SF
Sbjct: 142 DGFIDENELKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKIDFYEFVKLIEKSF 182

[Close]
BLAST of Spg036275 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 68.9 bits (167), Expect = 7.1e-12
Identity = 28/73 (38.36%), Postives = 53/73 (72.60%), Query Frame = 0 

Query: 201 DEELSLGEVEEAFDLFDENGDGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDG 260
           ++E+   +++EAF++FD NGDGFI  ++LK VL ++G      +++CR+MI   D +GDG
Sbjct: 74  EDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDG 133

Query: 261 RLDFEEFAKLVEQ 274
           R+++ EF +++++
Sbjct: 134 RVNYMEFRQMMKK 146

[Close]
BLAST of Spg036275 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 68.9 bits (167), Expect = 7.1e-12
Identity = 31/65 (47.69%), Postives = 47/65 (72.31%), Query Frame = 0 

Query: 208 EVEEAFDLFDENGDGFIDAEDLKNVLCAMGFTVASEVDQCRRMISPFDNNGDGRLDFEEF 267
           ++EEAF++FDE+GDGFI A +L+ VL  +G   A E++Q  +MI   D+N DGR+DF EF
Sbjct: 111 DLEEAFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEF 170

Query: 268 AKLVE 273
             +++
Sbjct: 171 KNMMQ 175

The following BLAST results are available for this feature:
BLAST of Spg036275 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038895011.18.8e-7379.43probable calcium-binding protein CML45 [Benincasa hispida][more]
KAG7020054.12.2e-6878.86putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. a... [more]
KAG6584466.12.9e-6876.37putative calcium-binding protein CML46, partial [Cucurbita argyrosperma subsp. s... [more]
XP_023001351.15.0e-6878.29probable calcium-binding protein CML47 [Cucurbita maxima][more]
XP_023519469.15.0e-6878.86probable calcium-binding protein CML47 [Cucurbita pepo subsp. pepo][more]
BLAST of Spg036275 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9MBG51.9e-1741.53Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ... [more]
Q93Z277.1e-1740.60Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ... [more]
Q9SN892.5e-1440.00Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ... [more]
Q2QVI15.3e-1249.21Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q0JC442.6e-1143.66Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
BLAST of Spg036275 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1KID52.4e-6878.29probable calcium-binding protein CML47 OS=Cucurbita maxima OX=3661 GN=LOC1114955... [more]
A0A5A7T3X09.2e-6874.43Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3BEM16.0e-6773.86probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103488781 P... [more]
A0A6J1ECX53.0e-6678.36probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1C8B52.1e-5675.00uncharacterized protein LOC111008903 isoform X1 OS=Momordica charantia OX=3673 G... [more]
BLAST of Spg036275 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G29000.11.3e-1841.53Calcium-binding EF-hand family protein [more]
AT5G39670.15.1e-1840.60Calcium-binding EF-hand family protein [more]
AT3G47480.11.8e-1540.00Calcium-binding EF-hand family protein [more]
AT4G03290.17.1e-1238.36EF hand calcium-binding protein family [more]
AT5G44460.17.1e-1247.69calmodulin like 43 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 168..202
NoneNo IPR availableGENE3D1.10.238.10coord: 122..276
e-value: 1.5E-20
score: 76.0
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 152..275
NoneNo IPR availablePANTHERPTHR10891:SF907CALCIUM-BINDING PROTEIN CML47-RELATEDcoord: 152..275
IPR002048EF-hand domainSMARTSM00054efh_1coord: 208..236
e-value: 7.2E-5
score: 32.2
coord: 246..274
e-value: 0.0031
score: 26.7
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 210..271
e-value: 1.8E-11
score: 44.3
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 242..276
score: 9.854874
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 204..239
score: 14.736568
IPR002048EF-hand domainCDDcd00051EFhcoord: 208..271
e-value: 9.95808E-16
score: 67.9581
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 217..229
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 255..267
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 165..272

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg036275.1Spg036275.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006464 cellular protein modification process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005509 calcium ion binding