Spg036206 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg036206
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	scaffold5: 45069415 .. 45072346 (+)
RNA-Seq Expression	Spg036206
Synteny	Spg036206

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAGGGAAGACTCAAAAGTTGGTTATAGGGTAATTATTATTAGGAGAGAGCAAGGAAAATGGAGTGGTCTTGGTAGTAGGAGATAAAACTTTCATGGTTTTTTTTTTTTTTTTAATAATTTTTTTTTGGGGTGTGTATAAATATTTAGAGTATGGTTTTTGTGGAGGGTGTTTTGAGAGATTGAAGAGTGATGAAGGAGACTTTAAGGTATTTGGTTGGAATTGCAGGGCCAAGTGGGTATGGCTCTAAATCCACAGCAGAGCAAGTTACTCAACATTTTCAGTTTTCTTCTCCTTTGACTGCCATTATCACTGGTTAGCATCTCAAAACCTTTTACAAATACAACTCTATTAAAAGAAATTCCTCTCTCTCTCTCTCTATTGTGCTAATCACTTCAGTTGTGGTCTTTTGTACTGTTATTTTGTATGGTGCTGAGTTCAAAGTTGTGTTCACTTGCAAAGTTGAGGCATCTTTTTGTAGGGGAGACCTATAAGAAAAGCATAAAAAGGATTTGAATAACCGAACTGAATTGTCTTATGATTTAGGTTACCATATGAAATGAGGTTAAAGGGTCTAGTTATGCTTCTTGTATCATTTCTCATAATTTTCTTTTCTGAGTTTACCAATATGTAGAGTGAGGATTCAAATCTCTTACCTCTCAGTCGAGATTATAATACCTTAACCAATTGAGCTAGATTTTATAATGCCTTCTAGTCGAGACTTCATTTTCATTATTTGGTTTGTATGAATCGAAGAGGGTCTTGTTCCAATATTTTCCTAATTAATTCGTGCATTCACCTTCAAAACTTATCAGTCAATATATATACATACTTCTGTAGAAAGTTTAATTATACATATCCATGGAAATAAGGTGAAAAATCTTGACATTATCAATCAAACCATATAATGTTGTTACCAAAGAGAAGGACACAAGGGTTCAAAAGATATAGCTCTTAAAACAAGACAATAGAATCCACAAGAATGATGAGAAAACAATAAATCAAACAAGGAAAACTATACATTGATTGTTAAATACTAATAAAGAAATTGTTTCTATTGTGTATGAAAGGGGCAACATCAGGCATTGGAGAAGAAACAGCAAGAGTATTAGCAAAGAGAGGAGTGAGAATAGTAATAGCAGCAAGAGACATGAAGAAGGCAAGTAAAGTGAAAGAGAGGATCAAAAGAGAAAGAGCAGAGGCACAAATTTTAGTGTTAGAGATAGACCTCAGCTCTTTGGCTTCTGTAAGGAGGTTCTGTATGGAGTTTGCAGCTTTAGGGCTACCACTCAACATTCTCATGTGAGTTTGTCTTTACTTTTCCCAACCCAATTCATATTTTCCCTCTCAAACTTCAACCCTTTTTGAGACATTTGTTTTTTCAGAAACAATGGTGGGGTTTTCTCTCCAAATTTGGAGTATTCTGAAGACAAATTTGAATTGACATTTGCGACTAATTATTTGGGTAATCCTTTTTCTCTTCTTCTTTTCTTTCTTGTCATTCTTAAACTCAAACAGAGAAGCTCCTCCATGTAGAGTATCAACTCTAGAAACACCTCAATGTCATTTGTAAGCAAAGATTAAAATGATAATACTTCTTCAAAGTTGGAACACTTTTTAGAAAGTTGAGTTTACTACTCGATAGTGGCTTAAGAAGACTTAAGTTATGTCAAATTTAACATCAAAGGATGCAGTGTTTCAATCTCACTGTCTCTTTTCCTTGCAGGACATTACCTTCTGACAGAACTGCTTTTAGACAAATTGATAGAAACATCAAAGAGAACAGGCATCCAAGGAAGAATTATAAACGTCTCTTCTGTTGTCCACTGCTGGGTGAAGAAGGACGGTCTCTGCTTCAGCCACCTGCTCAACCCAAACAAGTAAGAAAACTTGCTCCCTTTTTGGATTCTTTTCTTTCTGGGTTGTTTTCTTTCCTCAAAACACAAAAAGTTGTCTAGCTGGTTTCTGGTAAAAAAATCAGCCATGGTTAGAGAATCAAACTAAACCCTGTTTGGAATTTGGGATTTCTGCAGATACAATGGCACGCGCGCTTATGCTCAGTCAAAACTGGCTTGCATTTTACATGCCAAGGAGGTGGCCAGACAGTTGAAGGTCATCTTGCTTCTTCTTCACCTTCTACATTTCCTGCAAACAATTAATAAACAGCCCATGTTCTGAAATCCCAGTCATTTTTACAGGAAAGAGATGCCAATGTGACAATAAATGCAGTGCATCCAGGAATTGTAAAGACTGCAATTATCAGGGCACATCGAGGATTTATCACAGGCATGAACTATTGACTCTTTCTAATCCCTGATCTACTGTTATCTTAAACTAGTTCAACCTAAAGGAAAATGCAGAAGAGGGTATTGAATTATTATTAACTATATTCTAACATCTTTTGCAGATTCTCTCTTTTTCATGGCATCGAAGTTACTAAAGTCCATTCCTCAGGTAATTTCTTATATAAAAGCTGTGCAGAGGGCTTGAATTTGCACAATGCTTTATCATACAAACATTAATAACAATGGCCGTGCATGCAGGGTGCTGCTACAACATGCTATGTGGCTTTAAGCCCACAAACAGAAAGAAAAACAGGGAAATATTTCGCAGATTGTAATGAAACCAAGTGTTCTTCTCTAGCCAATGACGAGTTAGCAGCACGAACGCTATGGATGCAAACTCGTGCCCTAATTCTCAGAGAACTTGGTGAACCAGCTTGAACTACTTAAATATGCTTTCTATATCATCATCAATAATTTTTCAAACTAGAGAATAGTATATATAATTCTATGCACTTGTAAATGAAACGAGATCCATGACAAGTTCCTTGAATAAGATCTCATAATAAAATATGCCCATTTTTCTTAAACAACTCCCTCAATCTGTAAGAAGTTGAACTTCGTATATGAAGAATAATTTCTAAACCCC

mRNA sequence

ATGAAGGAGACTTTAAGGTATTTGGTTGGAATTGCAGGGCCAAGTGGGTATGGCTCTAAATCCACAGCAGAGCAAGTTACTCAACATTTTCAGTTTTCTTCTCCTTTGACTGCCATTATCACTGGGGCAACATCAGGCATTGGAGAAGAAACAGCAAGAGTATTAGCAAAGAGAGGAGTGAGAATAGTAATAGCAGCAAGAGACATGAAGAAGGCAAGTAAAGTGAAAGAGAGGATCAAAAGAGAAAGAGCAGAGGCACAAATTTTAGTGTTAGAGATAGACCTCAGCTCTTTGGCTTCTGTAAGGAGGTTCTGTATGGAGTTTGCAGCTTTAGGGCTACCACTCAACATTCTCATAAACAATGGTGGGGTTTTCTCTCCAAATTTGGAGTATTCTGAAGACAAATTTGAATTGACATTTGCGACTAATTATTTGGGACATTACCTTCTGACAGAACTGCTTTTAGACAAATTGATAGAAACATCAAAGAGAACAGGCATCCAAGGAAGAATTATAAACGTCTCTTCTGTTGTCCACTGCTGGGTGAAGAAGGACGGTCTCTGCTTCAGCCACCTGCTCAACCCAAACAAATACAATGGCACGCGCGCTTATGCTCAGTCAAAACTGGCTTGCATTTTACATGCCAAGGAGGTGGCCAGACAGTTGAAGGAAAGAGATGCCAATGTGACAATAAATGCAGTGCATCCAGGAATTGTAAAGACTGCAATTATCAGGGCACATCGAGGATTTATCACAGATTCTCTCTTTTTCATGGCATCGAAGTTACTAAAGTCCATTCCTCAGGGTGCTGCTACAACATGCTATGTGGCTTTAAGCCCACAAACAGAAAGAAAAACAGGGAAATATTTCGCAGATTGTAATGAAACCAAGTGTTCTTCTCTAGCCAATGACGAGTTAGCAGCACGAACGCTATGGATGCAAACTCGTGCCCTAATTCTCAGAGAACTTGGTGAACCAGCTTGA

Coding sequence (CDS)

ATGAAGGAGACTTTAAGGTATTTGGTTGGAATTGCAGGGCCAAGTGGGTATGGCTCTAAATCCACAGCAGAGCAAGTTACTCAACATTTTCAGTTTTCTTCTCCTTTGACTGCCATTATCACTGGGGCAACATCAGGCATTGGAGAAGAAACAGCAAGAGTATTAGCAAAGAGAGGAGTGAGAATAGTAATAGCAGCAAGAGACATGAAGAAGGCAAGTAAAGTGAAAGAGAGGATCAAAAGAGAAAGAGCAGAGGCACAAATTTTAGTGTTAGAGATAGACCTCAGCTCTTTGGCTTCTGTAAGGAGGTTCTGTATGGAGTTTGCAGCTTTAGGGCTACCACTCAACATTCTCATAAACAATGGTGGGGTTTTCTCTCCAAATTTGGAGTATTCTGAAGACAAATTTGAATTGACATTTGCGACTAATTATTTGGGACATTACCTTCTGACAGAACTGCTTTTAGACAAATTGATAGAAACATCAAAGAGAACAGGCATCCAAGGAAGAATTATAAACGTCTCTTCTGTTGTCCACTGCTGGGTGAAGAAGGACGGTCTCTGCTTCAGCCACCTGCTCAACCCAAACAAATACAATGGCACGCGCGCTTATGCTCAGTCAAAACTGGCTTGCATTTTACATGCCAAGGAGGTGGCCAGACAGTTGAAGGAAAGAGATGCCAATGTGACAATAAATGCAGTGCATCCAGGAATTGTAAAGACTGCAATTATCAGGGCACATCGAGGATTTATCACAGATTCTCTCTTTTTCATGGCATCGAAGTTACTAAAGTCCATTCCTCAGGGTGCTGCTACAACATGCTATGTGGCTTTAAGCCCACAAACAGAAAGAAAAACAGGGAAATATTTCGCAGATTGTAATGAAACCAAGTGTTCTTCTCTAGCCAATGACGAGTTAGCAGCACGAACGCTATGGATGCAAACTCGTGCCCTAATTCTCAGAGAACTTGGTGAACCAGCTTGA

Protein sequence

MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGVRIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHCWVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSSLANDELAARTLWMQTRALILRELGEPA

Homology

[Close]

BLAST of Spg036206 vs. NCBI nr
Match: XP_023000696.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 605.1 bits (1559), Expect = 3.5e-169
Identity = 307/326 (94.17%), Postives = 319/326 (97.85%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGV 60
           MKETLRYLVGIAGPSGYGSKS+AEQVT+H Q SSPLTAIITGATSGIGEETARVLAKRGV
Sbjct: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIV+AARDMKKA+KV+ERIKRE  EA+ILV EIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYS+DK ELTFATNYLGHYLLTELLLDKLIETS+RTGIQGRIINVSSVVHC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKD LCF+HLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSS 300
           TAII+AHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQT+RKTG+YFADCNETKCSS
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSS 313

Query: 301 LANDELAARTLWMQTRALILRELGEP 327
           LANDELAARTLWMQTRALILRELGEP
Sbjct: 314 LANDELAARTLWMQTRALILRELGEP 339

[Close]

BLAST of Spg036206 vs. NCBI nr
Match: XP_022924073.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 597.8 bits (1540), Expect = 5.6e-167
Identity = 303/326 (92.94%), Postives = 317/326 (97.24%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGV 60
           MKETLRYLVG+AGPSGYGSKS+AEQVT+H Q SSPLTAIITGATSGIGEETARVLAKRGV
Sbjct: 14  MKETLRYLVGMAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIV+AARDMKKA+KV+ERIKR   EA+IL+ EIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKRGGPEAEILMSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYS+DK ELTFATNYLGHYLLTELLLDKLIETS+RTGIQGRIINVSSVVHC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKD LCF+HLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSS 300
           TAII+AHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQT RKTG+YFADCNETKCSS
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTHRKTGRYFADCNETKCSS 313

Query: 301 LANDELAARTLWMQTRALILRELGEP 327
           LANDELAARTLW+QTRALILRELGEP
Sbjct: 314 LANDELAARTLWIQTRALILRELGEP 339

[Close]

BLAST of Spg036206 vs. NCBI nr
Match: XP_038895224.1 (short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 597.0 bits (1538), Expect = 9.5e-167
Identity = 304/327 (92.97%), Postives = 318/327 (97.25%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGV 60
           MKETLRYLVGIAGPSGYGSK++AE+VT+ FQFSSP TAIITGATSGIGEETARVLAKRGV
Sbjct: 1   MKETLRYLVGIAGPSGYGSKASAEKVTESFQFSSPFTAIITGATSGIGEETARVLAKRGV 60

Query: 61  RIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIV+AARDMKKA+KVKERIKRE  EA+ILV EIDLSSLASVRRFC+EFAALGLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVKERIKRESPEAEILVSEIDLSSLASVRRFCVEFAALGLPLNILIN 120

Query: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVH 
Sbjct: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHY 180

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVK++ LCF HLLNPNKYNGTRAYAQSKLACILHAKEVAR+LKE+DANVTINAVHPGIVK
Sbjct: 181 WVKREDLCFIHLLNPNKYNGTRAYAQSKLACILHAKEVARKLKEQDANVTINAVHPGIVK 240

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSS 300
           TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQT+RKTGKYFADCN+TKCSS
Sbjct: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGKYFADCNKTKCSS 300

Query: 301 LANDELAARTLWMQTRALILRELGEPA 328
           LANDEL ARTLWMQTRALILRELGEPA
Sbjct: 301 LANDELTARTLWMQTRALILRELGEPA 327

[Close]

BLAST of Spg036206 vs. NCBI nr
Match: XP_023519347.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 596.7 bits (1537), Expect = 1.2e-166
Identity = 302/326 (92.64%), Postives = 316/326 (96.93%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGV 60
           MKETLRYLVGIAGPSGYGSKS+AEQVT+H Q SSPLTAIITGATSGIGEETARVLAKRG+
Sbjct: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60

Query: 61  RIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIV+AARDMKKA+KVKERIKRE  EA+ILV EIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120

Query: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYS+DK ELTFATNYLGHY++TELLLDKLIETSKRTGIQGRIINVSSVVHC
Sbjct: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKD LCF+HLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK
Sbjct: 181 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSS 300
           TAI++AHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQT RKTG+YFADCNETKCSS
Sbjct: 241 TAILKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSS 300

Query: 301 LANDELAARTLWMQTRALILRELGEP 327
           LANDELAAR LW+QTRALILREL EP
Sbjct: 301 LANDELAARKLWIQTRALILRELDEP 326

[Close]

BLAST of Spg036206 vs. NCBI nr
Match: XP_038895223.1 (short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 582.0 bits (1499), Expect = 3.2e-162
Identity = 304/355 (85.63%), Postives = 318/355 (89.58%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGV 60
           MKETLRYLVGIAGPSGYGSK++AE+VT+ FQFSSP TAIITGATSGIGEETARVLAKRGV
Sbjct: 1   MKETLRYLVGIAGPSGYGSKASAEKVTESFQFSSPFTAIITGATSGIGEETARVLAKRGV 60

Query: 61  RIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIV+AARDMKKA+KVKERIKRE  EA+ILV EIDLSSLASVRRFC+EFAALGLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVKERIKRESPEAEILVSEIDLSSLASVRRFCVEFAALGLPLNILIN 120

Query: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVH 
Sbjct: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHY 180

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLK----------------- 240
           WVK++ LCF HLLNPNKYNGTRAYAQSKLACILHAKEVAR+LK                 
Sbjct: 181 WVKREDLCFIHLLNPNKYNGTRAYAQSKLACILHAKEVARKLKVIFLLHLLHFLHRINKT 240

Query: 241 -----------ERDANVTINAVHPGIVKTAIIRAHRGFITDSLFFMASKLLKSIPQGAAT 300
                      E+DANVTINAVHPGIVKTAIIRAHRGFITDSLFFMASKLLKSIPQGAAT
Sbjct: 241 AHILRFQPFLQEQDANVTINAVHPGIVKTAIIRAHRGFITDSLFFMASKLLKSIPQGAAT 300

Query: 301 TCYVALSPQTERKTGKYFADCNETKCSSLANDELAARTLWMQTRALILRELGEPA 328
           TCYVALSPQT+RKTGKYFADCN+TKCSSLANDEL ARTLWMQTRALILRELGEPA
Sbjct: 301 TCYVALSPQTQRKTGKYFADCNKTKCSSLANDELTARTLWMQTRALILRELGEPA 355

[Close]

BLAST of Spg036206 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.4e-75
Identity = 155/305 (50.82%), Postives = 207/305 (67.87%), Query Frame = 0


Query: 8   LVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGVRIVIAAR 67
           + G AG SGYGS STAE VT H   +  LTAIITG TSGIG E ARVL  RG  ++IA+R
Sbjct: 7   VTGKAGKSGYGSASTAEDVT-HSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIASR 66

Query: 68  DMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSP 127
           + K A+  KE I +    A+I  L++DLSS+ SVR F  +F AL +PLNILINN GV   
Sbjct: 67  NTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFC 126

Query: 128 NLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHCWVKKDGL 187
             + SED  E  FATN++GH+LLT LLLDK+  +++ +GI+GRI+N+SS+ H +   +G+
Sbjct: 127 PFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGI 186

Query: 188 CFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIRAH 247
            F ++ +P++Y+  +AY QSKLA +LH+  ++R+L+E   N+TIN+VHPG++ T + R H
Sbjct: 187 MFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFR-H 246

Query: 248 RGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSSLANDELA 307
            G     L  M+  L K+IPQGAATTCYVAL P  +  TGKYFADCN T  S+ A D   
Sbjct: 247 SGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSNFAIDTTL 306

Query: 308 ARTLW 313
           A  LW
Sbjct: 307 ADKLW 309

[Close]

BLAST of Spg036206 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 1.8e-75
Identity = 155/305 (50.82%), Postives = 206/305 (67.54%), Query Frame = 0


Query: 8   LVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGVRIVIAAR 67
           + G AG SGYGS STAE VT H   +  LTAIITG TSGIG E ARVL  RG  ++IAAR
Sbjct: 7   VTGKAGKSGYGSASTAEDVT-HSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIAAR 66

Query: 68  DMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSP 127
           + K A+  KE I +    A+I  L++DLSS+ SVR F  +F AL +PLNILINN GV   
Sbjct: 67  NTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFC 126

Query: 128 NLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHCWVKKDGL 187
             + SED  E  FATN++GH+LLT LLLDK+  +++ +GI+GRI+N+SS+ H +   +G+
Sbjct: 127 PFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGI 186

Query: 188 CFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIRAH 247
            F ++ +P++Y+  +AY QSKLA +LH+  ++R+L+E   N+TIN+VHPG++ T + R H
Sbjct: 187 MFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFR-H 246

Query: 248 RGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSSLANDELA 307
            G     L  M+  L K+IPQGAATTCYVAL P  +  TGKYF DCN T  S+ A D   
Sbjct: 247 SGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSNFAIDTTL 306

Query: 308 ARTLW 313
           A  LW
Sbjct: 307 ADKLW 309

[Close]

BLAST of Spg036206 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.5e-71
Identity = 146/319 (45.77%), Postives = 210/319 (65.83%), Query Frame = 0


Query: 7   YLVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGVRIVIAA 66
           +  G  G SG+ S+STAE+VT H    + LTAI+TGA+SGIG ETARVL+ RGV +V+A 
Sbjct: 2   WFFGSKGASGFSSRSTAEEVT-HGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 61

Query: 67  RDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFS 126
           R+    +KVKE I ++   A++ V+E+DLSS+ SVR+F  E+ + GLPLN+LINN G+ +
Sbjct: 62  RNTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMA 121

Query: 127 PNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHCWVKKDG 186
                S+D  EL FATN+LGH+LLT+LLLD +  TS+ +  +GRI+N+SS  H +   +G
Sbjct: 122 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG 181

Query: 187 LCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIRA 246
           + F  + + + Y+  RAY QSKL  +LHA E+ +QLKE   N+T N++HPG + T + R 
Sbjct: 182 VRFDKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRY 241

Query: 247 HRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSSLANDEL 306
              ++  ++  +A  +LKS+PQGAATTCYVAL+PQ    +G+YF D N  K   L  D  
Sbjct: 242 FNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTE 301

Query: 307 AARTLWMQTRALILRELGE 326
            A+ +W  +  L   + GE
Sbjct: 302 LAKKVWDFSTKLTDSQSGE 319

[Close]

BLAST of Spg036206 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.5e-66
Identity = 135/300 (45.00%), Postives = 196/300 (65.33%), Query Frame = 0


Query: 13  GPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGVRIVIAARDMKKA 72
           G SG+   STAEQVT H   ++ LTAI+TGA+SGIG ET RVLA RG  +++  R+M  A
Sbjct: 9   GVSGFSGSSTAEQVT-HGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAA 68

Query: 73  SKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSPNLEYS 132
             VK+ I ++   A++  +E+DLSSL SV++F  EF + G PLNILINN G+ +   + S
Sbjct: 69  KDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFKLS 128

Query: 133 EDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHCWVKKDGLCFSHL 192
           +D  EL FATN++GH+LLT LLLD + +T++ +  +GRI+NV+S  H +   +G+ F  +
Sbjct: 129 KDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRFDKI 188

Query: 193 LNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIRAHRGFIT 252
            + + YN  RAY QSKLA +LHA ++ + LKE   N+T N++HPG + T + R H   + 
Sbjct: 189 NDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFR-HNSAVN 248

Query: 253 DSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSSLANDELAARTLW 312
             +  +   +LK++ QGAATTCYVAL PQ +  +G+YF+D N  K +    D   A+ LW
Sbjct: 249 GLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVDLAKKLW 306

[Close]

BLAST of Spg036206 vs. ExPASy Swiss-Prot
Match: P59837 (Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 6.9e-43
Identity = 112/274 (40.88%), Postives = 157/274 (57.30%), Query Frame = 0


Query: 39  IITGATSGIGEETARVLAKRGVRIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSL 98
           +ITGA +GIG+ETAR LA+RG R+ IA RD+ K       I+ +   +Q+LV ++DLS  
Sbjct: 43  VITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIQADTKNSQVLVRKLDLSDT 102

Query: 99  ASVRRFCMEFAALGLPLNILINNGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKL 158
            S+R F   F A    L+ILINN GV       + D FE   A N+LGH+LLT LLL +L
Sbjct: 103 KSIRAFAEGFLAEEKQLHILINNAGVMLCPYSKTADGFETHLAVNHLGHFLLTHLLLGRL 162

Query: 159 IETSKRTGIQGRIINVSSVVHCWVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEV 218
            E++       R++N+SSV H   K   + F  L     YN   AY  SKLA +L  +E+
Sbjct: 163 KESA-----PARVVNLSSVAHHLGK---IRFHDLQGDKYYNLGFAYCHSKLANVLFTREL 222

Query: 219 ARQLKERDANVTINAVHPGIVKTAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVAL 278
           A++LK     VT  AVHPGIV++ ++R    F+   L+ + S  LK+  +GA T+ + AL
Sbjct: 223 AKRLK--GTGVTTYAVHPGIVRSKLVR--HSFLLCLLWRLFSPFLKTTWEGAQTSLHCAL 282

Query: 279 SPQTERKTGKYFADCNETKCSSLANDELAARTLW 313
           +   E  +GKYF+DC +T  S  A +   A  LW
Sbjct: 283 AEGLEPLSGKYFSDCKKTWVSPRARNNKTAERLW 304

[Close]

BLAST of Spg036206 vs. ExPASy TrEMBL
Match: A0A6J1KGJ5 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495058 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.7e-169
Identity = 307/326 (94.17%), Postives = 319/326 (97.85%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGV 60
           MKETLRYLVGIAGPSGYGSKS+AEQVT+H Q SSPLTAIITGATSGIGEETARVLAKRGV
Sbjct: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIV+AARDMKKA+KV+ERIKRE  EA+ILV EIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYS+DK ELTFATNYLGHYLLTELLLDKLIETS+RTGIQGRIINVSSVVHC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKD LCF+HLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSS 300
           TAII+AHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQT+RKTG+YFADCNETKCSS
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSS 313

Query: 301 LANDELAARTLWMQTRALILRELGEP 327
           LANDELAARTLWMQTRALILRELGEP
Sbjct: 314 LANDELAARTLWMQTRALILRELGEP 339

[Close]

BLAST of Spg036206 vs. ExPASy TrEMBL
Match: A0A6J1EBB9 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111431615 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.7e-167
Identity = 303/326 (92.94%), Postives = 317/326 (97.24%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGV 60
           MKETLRYLVG+AGPSGYGSKS+AEQVT+H Q SSPLTAIITGATSGIGEETARVLAKRGV
Sbjct: 14  MKETLRYLVGMAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIV+AARDMKKA+KV+ERIKR   EA+IL+ EIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKRGGPEAEILMSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYS+DK ELTFATNYLGHYLLTELLLDKLIETS+RTGIQGRIINVSSVVHC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKD LCF+HLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSS 300
           TAII+AHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQT RKTG+YFADCNETKCSS
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTHRKTGRYFADCNETKCSS 313

Query: 301 LANDELAARTLWMQTRALILRELGEP 327
           LANDELAARTLW+QTRALILRELGEP
Sbjct: 314 LANDELAARTLWIQTRALILRELGEP 339

[Close]

BLAST of Spg036206 vs. ExPASy TrEMBL
Match: A0A6J1C5L3 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111008680 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.5e-162
Identity = 295/327 (90.21%), Postives = 311/327 (95.11%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGV 60
           MKETLRYL GIAG SGYGSKSTAEQVTQH  FSSPLTAIITGATSGIGEETARV+AKRGV
Sbjct: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60

Query: 61  RIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIVIAARD+KKA+KVKERIKRE AEA+ILV EIDLSSLASVRRFC++FAA GLPLNILIN
Sbjct: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120

Query: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           N GVFSPNLEYS+DKFELTFATNYLGHYLLTE+LLD LIETSKRTGIQGRIINVSSVVHC
Sbjct: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180

Query: 181 WVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240
           WVKKDGLCF+HLLNPNKYN T AYAQSKLACILHAKEVARQLKER ANVT+NA+HPGIVK
Sbjct: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240

Query: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSS 300
           TAIIRAH GFITDSLFFMASKLLKSIPQGAATTCYVAL+PQTERKTGKYF+DCNETKCS+
Sbjct: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300

Query: 301 LANDELAARTLWMQTRALILRELGEPA 328
           LAN+ELAAR LWMQTRALI R+LGE A
Sbjct: 301 LANNELAARKLWMQTRALIHRQLGETA 327

[Close]

BLAST of Spg036206 vs. ExPASy TrEMBL
Match: A0A6J1BYZ0 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111006882 PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.9e-137
Identity = 255/332 (76.81%), Postives = 290/332 (87.35%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFSS-----PLTAIITGATSGIGEETARVL 60
           MKETLRYL GIAGPSGYGS STAEQVTQ++  SS     PLTAIITGATSGIG ETARVL
Sbjct: 15  MKETLRYLAGIAGPSGYGSNSTAEQVTQNYSISSSSTSHPLTAIITGATSGIGAETARVL 74

Query: 61  AKRGVRIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPL 120
           AKRGVRIV+AARD+KKA++VKE I++E  EA+I+V EIDLSSLASV+RFC +F ALGLPL
Sbjct: 75  AKRGVRIVMAARDLKKAAQVKEGIQKETPEAEIIVCEIDLSSLASVQRFCNQFLALGLPL 134

Query: 121 NILINNGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVS 180
           NILINN GVFS NLE+SEDK ELTFATNYLGHY LTE LL+K+IET+ +TGI+GRIINVS
Sbjct: 135 NILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETAAKTGIEGRIINVS 194

Query: 181 SVVHCWVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVH 240
           SV+HCWVKKDGL F  +LNPN YNGTRAYAQSKLA ILHAKE++RQL+ R+A VTINAVH
Sbjct: 195 SVIHCWVKKDGLRFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQARNARVTINAVH 254

Query: 241 PGIVKTAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNE 300
           PGIVKTAIIRAH+GFITDSLFFMASKLLK+  QGA+TTCYVALS QTE K+GK++ADCNE
Sbjct: 255 PGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNE 314

Query: 301 TKCSSLANDELAARTLWMQTRALILRELGEPA 328
           T CS+LA+DEL A+ LW +T  LI R L +PA
Sbjct: 315 TNCSALASDELEAQKLWTKTHNLIHRRLSKPA 346

[Close]

BLAST of Spg036206 vs. ExPASy TrEMBL
Match: A0A6J1JJF7 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485650 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 8.8e-134
Identity = 254/327 (77.68%), Postives = 284/327 (86.85%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFS--SPLTAIITGATSGIGEETARVLAKR 60
           MK+TLRYL GIAGPSGYGSKSTAEQVTQ   FS  S LTAIITGATSGIG ETARVLAKR
Sbjct: 17  MKDTLRYLAGIAGPSGYGSKSTAEQVTQCSSFSSTSQLTAIITGATSGIGAETARVLAKR 76

Query: 61  GVRIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNIL 120
           GVRIV+ ARDMKKA++VKE I+RE  EA+I+V EIDLSSLASV+ FC  F AL LPLNIL
Sbjct: 77  GVRIVMPARDMKKAAQVKEAIQRESPEAEIIVCEIDLSSLASVQSFCNHFLALRLPLNIL 136

Query: 121 INNGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVV 180
           INN GVFS NLE+SEDK ELTFATNYLGHY LTE LL+K+IET+ +TGI+GRIINVSSVV
Sbjct: 137 INNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETAAQTGIEGRIINVSSVV 196

Query: 181 HCWVKKDGLCFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGI 240
           H WVKKDGL F  +LNPN YNGTRAYAQSKLA ILHAKEV+RQL+ R+A VTINAVHPGI
Sbjct: 197 HGWVKKDGLSFGQMLNPNNYNGTRAYAQSKLANILHAKEVSRQLQGRNARVTINAVHPGI 256

Query: 241 VKTAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKC 300
           VKTAIIRAH+GFITDSLFFMASKLLK+  QGA+TTCYVALS QTE K+GK++ADCNET C
Sbjct: 257 VKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNETNC 316

Query: 301 SSLANDELAARTLWMQTRALILRELGE 326
           SSLANDE+ A+ LW +TR LI ++L +
Sbjct: 317 SSLANDEIEAQKLWTRTRNLINKQLSK 343

[Close]

BLAST of Spg036206 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 447.6 bits (1150), Expect = 8.7e-126
Identity = 234/327 (71.56%), Postives = 273/327 (83.49%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQF-SSPLTAIITGATSGIGEETARVLAKRG 60
           MK TLRYL GIAGP+G+GS+STAEQVTQH  F  S LTAIITG TSGIG ETARVLAKRG
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRG 60

Query: 61  VRIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILI 120
           VR+V+A RDMKKA  VKERI RE  EA I++ EIDLSSL+SV RFC +F +  LPLNILI
Sbjct: 61  VRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNILI 120

Query: 121 NNGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVH 180
           NN GVFSPNLE+SE+K ELTFATN+LGHYLLTE+L++K+I+T++++GI+GRIIN+SSV+H
Sbjct: 121 NNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIH 180

Query: 181 CWVKKDGLCFSHLLNP-NKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGI 240
            WVK D   F  LL+P ++YNGTRAYAQSKLA ILHAK +++QLK+R+ANVTINAVHPGI
Sbjct: 181 NWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGI 240

Query: 241 VKTAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKC 300
           VKT IIRAH+G  TDSLF +ASKLLKSI QGAATTCYVALS +T+  +GKYFADCNET C
Sbjct: 241 VKTGIIRAHKGLFTDSLFLIASKLLKSISQGAATTCYVALSNETKGLSGKYFADCNETNC 300

Query: 301 SSLANDELAARTLWMQTRALILRELGE 326
           S LANDE  A  L  Q+RALI   L E
Sbjct: 301 SDLANDEYVALKLCTQSRALIHDHLHE 327

[Close]

BLAST of Spg036206 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 378.3 bits (970), Expect = 6.5e-105
Identity = 196/270 (72.59%), Postives = 231/270 (85.56%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQF-SSPLTAIITGATSGIGEETARVLAKRG 60
           MK TLRYL GIAGP+G+GS+STAEQVTQH  F  S LTAIITG TSGIG ETARVLAKRG
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRG 60

Query: 61  VRIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILI 120
           VR+V+A RDMKKA  VKERI RE  EA I++ EIDLSSL+SV RFC +F +  LPLNILI
Sbjct: 61  VRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNILI 120

Query: 121 NNGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVH 180
           NN GVFSPNLE+SE+K ELTFATN+LGHYLLTE+L++K+I+T++++GI+GRIIN+SSV+H
Sbjct: 121 NNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIH 180

Query: 181 CWVKKDGLCFSHLLNP-NKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGI 240
            WVK D   F  LL+P ++YNGTRAYAQSKLA ILHAK +++QLK+R+ANVTINAVHPGI
Sbjct: 181 NWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGI 240

Query: 241 VKTAIIRAHRGFITDSLFFMASKLLKSIPQ 269
           VKT IIRAH+G  TDSLF +ASKLLKSI Q
Sbjct: 241 VKTGIIRAHKGLFTDSLFLIASKLLKSISQ 270

[Close]

BLAST of Spg036206 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 341.3 bits (874), Expect = 8.8e-94
Identity = 181/321 (56.39%), Postives = 231/321 (71.96%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGV 60
           M ET+++L+G  GPSG+GS+STA+ VT +    S LTAIITGATSGIG ETARVLAKRG 
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNSDLRS-LTAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           R+V+ AR +K A + K RI  E  +A+I+V+ +DLSSL SVRRF  +F +L LPLNILIN
Sbjct: 61  RLVLPARSVKTAEETKARILSEFPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNILIN 120

Query: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           N G ++     SED  E+TFATNYLGH+LLT+LLL K+IET+ +TG+QGRI+NV+SVVH 
Sbjct: 121 NAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVVHS 180

Query: 181 WVKKDGLCFSHLLNPN--KYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGI 240
           W   D L +   ++ N   Y+ TRAYA SKLA +LH  E++R L + DANVT N VHPGI
Sbjct: 181 WFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHPGI 240

Query: 241 VKTAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKC 300
           VKT + R   G +TD +FF+ SKLLKS+PQ AATTCYVA SP+     GKYF+DCNE + 
Sbjct: 241 VKTRLTRDREGVVTDLVFFLTSKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDCNEARS 300

Query: 301 SSLANDELAARTLWMQTRALI 320
           S   +  L A+ LW  +  L+
Sbjct: 301 SKSGSCNLKAQRLWTASDLLV 320

[Close]

BLAST of Spg036206 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 4.8e-92
Identity = 172/321 (53.58%), Postives = 234/321 (72.90%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGV 60
           M ET +YL+G AG SG+GSKSTAE+VT++    S +TA+ITGATSGIG ETARVLAKRG 
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCDLRS-ITAVITGATSGIGAETARVLAKRGA 60

Query: 61  RIVIAARDMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           R++  AR++K A + KERI  E  E +I+V+++DLSS+ASVR F  +F +L LPLN+LIN
Sbjct: 61  RLIFPARNVKAAEEAKERIVSEFPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLLIN 120

Query: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           N G  +     SED  E+TFATNYLGH+LLT LLL+K+I+T++ TG+QGRI+NV+S +H 
Sbjct: 121 NAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGIHG 180

Query: 181 WVKKDGLCFSHLLNPNK--YNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGI 240
           W   D + +  L++  K  ++ TRAYA SKLA +LH KE++ +L++  ANVT+N VHPG+
Sbjct: 181 WFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHPGV 240

Query: 241 VKTAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKC 300
           V+T + R   G +TD +FF+ASKL+K++PQ AATTCYVA +P+    +GKYF DCNET  
Sbjct: 241 VRTRLTRDREGLLTDLVFFLASKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDCNETTP 300

Query: 301 SSLANDELAARTLWMQTRALI 320
           S L  +   A  LW  +  L+
Sbjct: 301 SGLGTNSSEATKLWAASEILV 320

[Close]

BLAST of Spg036206 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.6e-82
Identity = 161/305 (52.79%), Postives = 210/305 (68.85%), Query Frame = 0


Query: 8   LVGIAGPSGYGSKSTAEQVTQHFQFSSPLTAIITGATSGIGEETARVLAKRGVRIVIAAR 67
           + G  GPSG+GS STAE+VTQ    ++ LTAIITG T GIG ETARVL+KRG  +VI AR
Sbjct: 7   ITGRRGPSGFGSASTAEEVTQGID-ATNLTAIITGGTGGIGMETARVLSKRGAHVVIGAR 66

Query: 68  DMKKASKVKERIKRERAEAQILVLEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSP 127
           +M  A   K  I R+ A A++ +L++DLSS+ S++ F  EF AL LPLN+LINN GV   
Sbjct: 67  NMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNAGVMFC 126

Query: 128 NLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHCWVKKDGL 187
             + SED  EL FATN++GH+LLT LLLD +  T+K +G++GRI+NVSSV H +  ++G+
Sbjct: 127 PYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYTYQEGI 186

Query: 188 CFSHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIRAH 247
            F  + +   Y+  RAY QSKLA ILHA E++RQL+E   N+T N+VHPG++ T + + H
Sbjct: 187 QFDSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGLILTNLFQ-H 246

Query: 248 RGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTERKTGKYFADCNETKCSSLANDELA 307
              +   L F +  L K+IPQGAATTCYVAL P  +  TGKYFADCNE   S LA DE  
Sbjct: 247 TALLMRFLKFFSFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARDETL 306

Query: 308 ARTLW 313
           A+ LW
Sbjct: 307 AQKLW 309

The following BLAST results are available for this feature:

BLAST of Spg036206 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023000696.1	3.5e-169	94.17	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita maxima]	[more]
XP_022924073.1	5.6e-167	92.94	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata]	[more]
XP_038895224.1	9.5e-167	92.97	short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa his...	[more]
XP_023519347.1	1.2e-166	92.64	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo...	[more]
XP_038895223.1	3.2e-162	85.63	short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X1 [Benincasa his...	[more]

BLAST of Spg036206 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078IS66	1.4e-75	50.82	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078ISJ6	1.8e-75	50.82	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A2RVM0	3.5e-71	45.77	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6RVV4	1.5e-66	45.00	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
P59837	6.9e-43	40.88	Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1	[more]

BLAST of Spg036206 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KGJ5	1.7e-169	94.17	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661...	[more]
A0A6J1EBB9	2.7e-167	92.94	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1C5L3	4.5e-162	90.21	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3...	[more]
A0A6J1BYZ0	2.9e-137	76.81	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3...	[more]
A0A6J1JJF7	8.8e-134	77.68	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max...	[more]

BLAST of Spg036206 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50130.1	8.7e-126	71.56	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.2	6.5e-105	72.59	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	8.8e-94	56.39	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24050.1	4.8e-92	53.58	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	2.6e-82	52.79	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 69..89
None	No IPR available	GENE3D	3.40.50.720		coord: 23..322 e-value: 1.4E-73 score: 249.5
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 39..242 e-value: 1.8E-11 score: 41.6
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 4..322
None	No IPR available	PANTHER	PTHR24320:SF198	NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN	coord: 4..322
None	No IPR available	CDD	cd05327	retinol-DH_like_SDR_c_like	coord: 37..312 e-value: 1.79639E-90 score: 269.479
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 113..124 score: 39.38 coord: 204..223 score: 27.97 coord: 227..244 score: 33.16 coord: 163..179 score: 32.79 coord: 37..54 score: 44.95
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 113..124 score: 38.63 coord: 169..177 score: 61.15 coord: 204..223 score: 26.08
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 37..178 e-value: 1.5E-27 score: 96.3
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 37..297

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg036206.1	Spg036206.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity