Spg036195 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg036195
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	scaffold5: 46084875 .. 46086261 (+)
RNA-Seq Expression	Spg036195
Synteny	Spg036195

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTCCTTGTAATTTAGAGACCTTGTTTTTGTGTCCAACAATGGCGGGTCAGCTCCCAGTTGTGAAGTTTTGTTCAGAGAATTTGAAGAGTGGAACGAGGAATTGGGTTTTGACGAGGGAGAAAGTTCGAGAAGCACTTGAAGAATATGGTTGTTTTGTTGCTTTATACGACAGTGTTTCAATGGAATTGAGTTCCAACATGTTTGATTCCTTAAAAGAGCTGTTTGATGTTCCATTGGATAGGAAGCTTCAGAATGTGTCTGAAAAGCCCTACCATGGCTACTTTGGCCAAAACCCTTTGATGCCCATCCATGAAAGCATGGGTATTGAAGACCCAATTCTCTTAACCAAAACTCAATCCTTCACTAACCTCATGTGGCCCTCTAATGGAAACAAACGTTTTTGGTAAGTTCTTTTTATCTCTCTAAGACTTTCATAAAATTGGCAAACTTGTTTTTATGAATATGAAATTAACCATTTTCATTATGCAGTGAAAACGTCAACTCCTATGCGAAACTGGTATCGTTGTTGGATCAGACGGTGAAAAATATGGTCTTTGAAAGCTATGGCGTGAGAAATCACTTTGATTCTCACGTGAAATCCACCAAATATCTTTTGCGAATGATCAAGTACAGGGTTCCGCAACAAAACGAGATGAATTTGGGAGCATTCCCTCACACAGACAAGAGCTTTCTCACTATTCTTCACCAAAATCAAGTCAATGGGTTGCATATCAAAACCAAAGATAACAAATGGCTTGAATATGAGCCTTCCTATTTTTCATCTTCTACCTCATCTTTCATCGTCATGGCTGGAGATGCATTCTTTGTAAGTTTCTTAACTTTTAGATTTCTTCGTCGTATTTTAAGATTATATAAAGAAAAAAAAAAGATTAAATTTAACATAATATATCAAATAATTAAGTGGTGAATTAACATAAAGTTTGTTCTTTTGTTGGCACAGGCATGGAGTAATGGGAGGATTTATTCACCACCTCATAGAGTGATTATGAGTGGGAAAAGAGAAAGATATTCATTGGGATTGTTTTCATTTAATGCTGGGATTGTTCAGATTCCTAAAGAGTTGGTAGACGACAAACACCCTCAACAATTTAAGCCATTTGATCATCAAGATTATCTCCGTTTTTATAGCACTGAGAGAGGTCAAAACTCCCCTTGTGCTATCAAGGCTTATTGTGGTGTTGGAAATGGCCTCCTCGATACTCGAGGATGCATTCTCTTACATTGATCCATATCTATTTAATCTTGTATTTTGTAAAGAAGTTTTTGTCGTTAGAAGGGATCGTCTCACCCTTAGGATGCCTATTTGAGATGCTTTTTTTTTTCATCCTCCTTTTCTTTTCCTGTGTATAACGTTGTGAAAATCC

mRNA sequence

ATGGCGGGTCAGCTCCCAGTTGTGAAGTTTTGTTCAGAGAATTTGAAGAGTGGAACGAGGAATTGGGTTTTGACGAGGGAGAAAGTTCGAGAAGCACTTGAAGAATATGGTTGTTTTGTTGCTTTATACGACAGTGTTTCAATGGAATTGAGTTCCAACATGTTTGATTCCTTAAAAGAGCTGTTTGATGTTCCATTGGATAGGAAGCTTCAGAATGTGTCTGAAAAGCCCTACCATGGCTACTTTGGCCAAAACCCTTTGATGCCCATCCATGAAAGCATGGGTATTGAAGACCCAATTCTCTTAACCAAAACTCAATCCTTCACTAACCTCATGTGGCCCTCTAATGGAAACAAACGTTTTTGTGAAAACGTCAACTCCTATGCGAAACTGGTATCGTTGTTGGATCAGACGGTGAAAAATATGGTCTTTGAAAGCTATGGCGTGAGAAATCACTTTGATTCTCACGTGAAATCCACCAAATATCTTTTGCGAATGATCAAGTACAGGGTTCCGCAACAAAACGAGATGAATTTGGGAGCATTCCCTCACACAGACAAGAGCTTTCTCACTATTCTTCACCAAAATCAAGTCAATGGGTTGCATATCAAAACCAAAGATAACAAATGGCTTGAATATGAGCCTTCCTATTTTTCATCTTCTACCTCATCTTTCATCGTCATGGCTGGAGATGCATTCTTTGCATGGAGTAATGGGAGGATTTATTCACCACCTCATAGAGTGATTATGAGTGGGAAAAGAGAAAGATATTCATTGGGATTGTTTTCATTTAATGCTGGGATTGTTCAGATTCCTAAAGAGTTGGTAGACGACAAACACCCTCAACAATTTAAGCCATTTGATCATCAAGATTATCTCCGTTTTTATAGCACTGAGAGAGGTCAAAACTCCCCTTGTGCTATCAAGGCTTATTGTGGTGTTGGAAATGGCCTCCTCGATACTCGAGGATGCATTCTCTTACATTGA

Coding sequence (CDS)

ATGGCGGGTCAGCTCCCAGTTGTGAAGTTTTGTTCAGAGAATTTGAAGAGTGGAACGAGGAATTGGGTTTTGACGAGGGAGAAAGTTCGAGAAGCACTTGAAGAATATGGTTGTTTTGTTGCTTTATACGACAGTGTTTCAATGGAATTGAGTTCCAACATGTTTGATTCCTTAAAAGAGCTGTTTGATGTTCCATTGGATAGGAAGCTTCAGAATGTGTCTGAAAAGCCCTACCATGGCTACTTTGGCCAAAACCCTTTGATGCCCATCCATGAAAGCATGGGTATTGAAGACCCAATTCTCTTAACCAAAACTCAATCCTTCACTAACCTCATGTGGCCCTCTAATGGAAACAAACGTTTTTGTGAAAACGTCAACTCCTATGCGAAACTGGTATCGTTGTTGGATCAGACGGTGAAAAATATGGTCTTTGAAAGCTATGGCGTGAGAAATCACTTTGATTCTCACGTGAAATCCACCAAATATCTTTTGCGAATGATCAAGTACAGGGTTCCGCAACAAAACGAGATGAATTTGGGAGCATTCCCTCACACAGACAAGAGCTTTCTCACTATTCTTCACCAAAATCAAGTCAATGGGTTGCATATCAAAACCAAAGATAACAAATGGCTTGAATATGAGCCTTCCTATTTTTCATCTTCTACCTCATCTTTCATCGTCATGGCTGGAGATGCATTCTTTGCATGGAGTAATGGGAGGATTTATTCACCACCTCATAGAGTGATTATGAGTGGGAAAAGAGAAAGATATTCATTGGGATTGTTTTCATTTAATGCTGGGATTGTTCAGATTCCTAAAGAGTTGGTAGACGACAAACACCCTCAACAATTTAAGCCATTTGATCATCAAGATTATCTCCGTTTTTATAGCACTGAGAGAGGTCAAAACTCCCCTTGTGCTATCAAGGCTTATTGTGGTGTTGGAAATGGCCTCCTCGATACTCGAGGATGCATTCTCTTACATTGA

Protein sequence

MAGQLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFDVPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFCENVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNLGAFPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNGRIYSPPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQNSPCAIKAYCGVGNGLLDTRGCILLH

Homology

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BLAST of Spg036195 vs. NCBI nr
Match: XP_022137575.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Momordica charantia])

HSP 1 Score: 599.0 bits (1543), Expect = 2.5e-167
Identity = 283/325 (87.08%), Postives = 304/325 (93.54%), Query Frame = 0


Query: 4   QLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFD 63
           QLP+VKF  ENL    R+WV TREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELF+
Sbjct: 5   QLPLVKFSEENL----RSWVSTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFE 64

Query: 64  VPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFCE 123
           VPL+RKL+NVSEKPYHGYFGQNPLMPIHESMGIEDP LL+KTQSFTNLMWPSNGNK FCE
Sbjct: 65  VPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGNKSFCE 124

Query: 124 NVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNLGAFP 183
           NVNSYA+ ++ LD+TVK MVFESYGVR H +SH+KST+YLLRMIKYRVP+QNEMN+GAFP
Sbjct: 125 NVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMNMGAFP 184

Query: 184 HTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNGRIYS 243
           HTDKSFLTILHQNQVNGL IKTK NKWL+Y PS  SSSTSSFIVMAGDAFFAWSNGRIYS
Sbjct: 185 HTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPSSSSSSTSSFIVMAGDAFFAWSNGRIYS 244

Query: 244 PPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQN 303
           PPH+VIMSGKRERYS+GLFSFN GIVQIPKELVD+ HPQQFKPFDHQDYLRFYSTERGQN
Sbjct: 245 PPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYSTERGQN 304

Query: 304 SPCAIKAYCGVGNGLLDTRGCILLH 329
           SPCAIKAYCG+GNGL+DTRGCILLH
Sbjct: 305 SPCAIKAYCGIGNGLVDTRGCILLH 325

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BLAST of Spg036195 vs. NCBI nr
Match: XP_038895877.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 563.1 bits (1450), Expect = 1.5e-156
Identity = 265/330 (80.30%), Postives = 296/330 (89.70%), Query Frame = 0


Query: 1   MAGQLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKE 60
           M G++P+VKFCSENLKSG + WVLTREKVREALEEYGCFVALYD+VSM++SSNMF SLK+
Sbjct: 1   MGGEIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQ 60

Query: 61  LFDVPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKR 120
           LFDVPL+RK+QNVSEKPYHGYFGQNPLMPIHESMGIE PIL T   SFTNLMWPS+GN+ 
Sbjct: 61  LFDVPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNRP 120

Query: 121 FCENVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNLG 180
           FCENV  YAKLVS LD+ VK+MVFESYGV     +H++STKYL+RMIKYRVP++ EMNLG
Sbjct: 121 FCENVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNLG 180

Query: 181 AFPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNGR 240
           AFPHTDKSFLTILHQN+VNGL IKT+DNKWL+Y P   SSSTSSFIVMAGDAFFAWSNGR
Sbjct: 181 AFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPP-SSSSTSSFIVMAGDAFFAWSNGR 240

Query: 241 IYSPPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTER 300
           IYSPPHRV++S  +ERYS+GLFSFN GI+QIPKELVDDKHPQQF PFDHQDYLRFY+TER
Sbjct: 241 IYSPPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTER 300

Query: 301 GQNSPCAIKAYCGVGNGLLDT--RGCILLH 329
           GQNSPCAIKAYCGVGN L+DT  R  +LLH
Sbjct: 301 GQNSPCAIKAYCGVGNSLVDTDSRSTLLLH 329

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BLAST of Spg036195 vs. NCBI nr
Match: XP_038893580.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 560.1 bits (1442), Expect = 1.3e-155
Identity = 264/327 (80.73%), Postives = 294/327 (89.91%), Query Frame = 0


Query: 4   QLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFD 63
           Q+P+VKFCSENLKSG + WVLTREKVREALEEYGCFVALYD+VSM++SSNMF SLK+LFD
Sbjct: 5   QIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQLFD 64

Query: 64  VPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFCE 123
           VPL+RK+QNVSEKPYHGYFGQNPLMPIHESMGIE PIL T   SFTNLMWPS+GN+ FCE
Sbjct: 65  VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNRPFCE 124

Query: 124 NVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNLGAFP 183
           NV  YAKLVS LD+ VK+MVFESYGV     +H++STKYL+RMIKYRVP++ EMNLGAFP
Sbjct: 125 NVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNLGAFP 184

Query: 184 HTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNGRIYS 243
           HTDKSFLTILHQN+VNGL IKT+DNKWL+Y P   SSSTSSFIVMAGDAFFAWSNGRIYS
Sbjct: 185 HTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPP-SSSSTSSFIVMAGDAFFAWSNGRIYS 244

Query: 244 PPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQN 303
           PPHRV++S  +ERYS+GLFSFN GI+QIPKELVDDKHPQQF PFDHQDYLRFY+TERGQN
Sbjct: 245 PPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTERGQN 304

Query: 304 SPCAIKAYCGVGNGLLDT--RGCILLH 329
           SPCAIKAYCGVGN L+DT  R  +LLH
Sbjct: 305 SPCAIKAYCGVGNSLVDTDSRSTLLLH 330

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BLAST of Spg036195 vs. NCBI nr
Match: TYJ99087.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa])

HSP 1 Score: 556.6 bits (1433), Expect = 1.4e-154
Identity = 269/331 (81.27%), Postives = 294/331 (88.82%), Query Frame = 0


Query: 1   MAGQLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKE 60
           M+ +LPVVKFCSENLKSGT+ WV T +KVREALE+YGCFVALYDSVS E+SSNMFDSLKE
Sbjct: 5   MSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKE 64

Query: 61  LF-DVPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNK 120
           LF DVPL+RKLQNVSEKPYHGYFGQNPLMPIHESMGIE PIL T   SFTNLMWPS GN 
Sbjct: 65  LFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGND 124

Query: 121 RFCENVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNL 180
            F E+V +YAKLVS LD+ VK MVFESYGV N   SH++STKYL+RMIKYRVP++ EMNL
Sbjct: 125 SFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNL 184

Query: 181 GAFPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQN+VNGL IKT+DNKW++Y P + SSSTSSFIVMAGDAFFAWSNG
Sbjct: 185 GAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHP-FSSSSTSSFIVMAGDAFFAWSNG 244

Query: 241 RIYSPPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTE 300
           RIYSPPHRVIMSG RERYS+GLFSFN GIVQIP+ELVDDKHPQ F PFDHQD+LRFYSTE
Sbjct: 245 RIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFYSTE 304

Query: 301 RGQNSPCAIKAYCGVGNGL--LDTRGCILLH 329
           RGQNSPCAIKAYCGVGN L  +DTR  +LLH
Sbjct: 305 RGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 334

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BLAST of Spg036195 vs. NCBI nr
Match: XP_004151475.3 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570.1 hypothetical protein Csa_013560 [Cucumis sativus])

HSP 1 Score: 556.2 bits (1432), Expect = 1.9e-154
Identity = 270/328 (82.32%), Postives = 292/328 (89.02%), Query Frame = 0


Query: 4   QLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFD 63
           QLPVVKFCS+NLKSGT  WV T +KVREALEEYGCFVALYD+VS E+SSNMFDSLKELFD
Sbjct: 12  QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLKELFD 71

Query: 64  -VPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFC 123
            VPL+RKLQNVSEKPYHGYFGQNPLMPIHESMGIE PIL T   SFTNLMWPS GN  F 
Sbjct: 72  EVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSFR 131

Query: 124 ENVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNLGAF 183
           ENV +YA LVS LD+ VK+MVFESYGV N   SH++STKYL+RMIKYRVP++ EMNLGAF
Sbjct: 132 ENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGAF 191

Query: 184 PHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNGRIY 243
           PHTDKSFLTILHQN+VNGL IKT+DNKW++Y P + SSSTSSFIVMAGDAFFAWSNGRIY
Sbjct: 192 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHP-FSSSSTSSFIVMAGDAFFAWSNGRIY 251

Query: 244 SPPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQ 303
           SPPHRVIMSG +ERYS+GLFSFN GIVQIPKELVDDKHPQ FKPFDHQDYLRFYSTERGQ
Sbjct: 252 SPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQ 311

Query: 304 NSPCAIKAYCGVGNGLL--DTRGCILLH 329
           NSPCAIKAYCGVGN L+  DTR  +LLH
Sbjct: 312 NSPCAIKAYCGVGNSLIDTDTRSPLLLH 338

[Close]

BLAST of Spg036195 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.3e-78
Identity = 139/315 (44.13%), Postives = 208/315 (66.03%), Query Frame = 0


Query: 4   QLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFD 63
           QLPV+ F  +NLK G+  W   +  V +ALE+YGCF A +D +S+EL+ ++F+++++LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFE 72

Query: 64  VPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFCE 123
           +P+  K +NVS KP+HGY   N    ++ES+GI+D  +L K   FT  +WP +GNK   E
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 124 NVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVP-----QQNEMN 183
            ++ +++ +  LD  V+ M+ ES+G+ N+ D H+ ST YL R++KY  P        E  
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEETK 192

Query: 184 LGAFPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSN 243
           LG   HTDK+ +TILHQ QV+GL +KTKD+KW++ +P     S  S +VM GD+  A  N
Sbjct: 193 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALLN 252

Query: 244 GRIYSPPHRVIMSGKRERYSLGLFSF--NAGIVQIPKELVDDKHPQQFKPFDHQDYLRFY 303
           GR++SP HRVIM+GK+ RYS GLFS      I+  P+ELVD +HP+ FKPF++ D+L F+
Sbjct: 253 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 312

Query: 304 STERGQNSPCAIKAY 312
            TE G+ +  A+ A+
Sbjct: 313 QTEAGRIAQSALHAF 318

[Close]

BLAST of Spg036195 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 1.9e-77
Identity = 139/316 (43.99%), Postives = 206/316 (65.19%), Query Frame = 0


Query: 4   QLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFD 63
           QLPV+ F  +NLK G+  W      V +ALE+YGCF A +D +S+EL+ ++F+++++LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 64  VPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFCE 123
           +P+  K +NVS KP+HGY   N    ++ES+GI D  +L K   FT  +WP +GNK   E
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 124 NVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVP------QQNEM 183
            ++ +++ +  LD  V+ M+ ES+G+ N+ D H+ ST YL R++KY  P         E 
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEET 192

Query: 184 NLGAFPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWS 243
            LG   HTDK+ +TILHQ QV+GL +KTKD+KW++ +P     S  S +VM GD+  A  
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALL 252

Query: 244 NGRIYSPPHRVIMSGKRERYSLGLFSF--NAGIVQIPKELVDDKHPQQFKPFDHQDYLRF 303
           NGR++SP HRVIM+GK+ RYS GLFS      I+  P+ELVD +HP+ FKPF++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 304 YSTERGQNSPCAIKAY 312
           + TE G+ +  A+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 319

[Close]

BLAST of Spg036195 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.2e-74
Identity = 135/305 (44.26%), Postives = 199/305 (65.25%), Query Frame = 0


Query: 4   QLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFD 63
           QLPV+ F  +NLK G+  W      V +ALE+YGCF A +D +S+EL+ ++F+++++LF+
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 65

Query: 64  VPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFCE 123
           +P+  K +NVS K +HGY   N    ++ES+GI D  +L K   FT  +WP +GNK   E
Sbjct: 66  LPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 125

Query: 124 NVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVP----QQNEMNL 183
            ++ +++ +  LD  V+ M+ ES+G+  + D H+ ST YL R++KY  P       E  L
Sbjct: 126 TIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETKL 185

Query: 184 GAFPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNG 243
           G   HTDK+ +TILHQ QV+GL +KTKD+KW++ +P     S  S +VM GD+  A  NG
Sbjct: 186 GLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALLNG 245

Query: 244 RIYSPPHRVIMSGKRERYSLGLFSF--NAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYS 303
           R++SP HRVIM+GK+ RYS GLFS      I+  P+ELVD +HP+ FKPF++ D+L F+ 
Sbjct: 246 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQ 301

[Close]

BLAST of Spg036195 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 6.8e-67
Identity = 130/308 (42.21%), Postives = 186/308 (60.39%), Query Frame = 0


Query: 4   QLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFD 63
           +LPV+    +NLK GT +W   R +VREALEEYGCF A+ D+VS EL   + +   EL +
Sbjct: 8   RLPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELLN 67

Query: 64  VPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFCE 123
           +PL+ K+ N ++  Y G+     +  ++E MG+     L K + FTNLMWP  GNK FCE
Sbjct: 68  LPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-EGNKDFCE 127

Query: 124 NVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNLGAFP 183
            V SY K ++ +D  +K MVFES+G+  HFDS  +ST YLL  ++Y+ P ++  +     
Sbjct: 128 TVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPALSL 187

Query: 184 HTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNGRIYS 243
           H DKS LTI++QN V GL  +TKD +W+         +  + IV+ GD F AWSNGR++S
Sbjct: 188 HKDKSILTIVNQNDVKGLEFETKDGEWI-------LPTADNHIVLLGDCFMAWSNGRLHS 247

Query: 244 PPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQN 303
           P HRV +   + R S   FSF   I++ P ELVD++HP  F PF+  + L +  T+ G  
Sbjct: 248 PLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAK 304

Query: 304 SPCAIKAY 312
           + C +K Y
Sbjct: 308 AVCDLKQY 304

[Close]

BLAST of Spg036195 vs. ExPASy Swiss-Prot
Match: Q9ZTA1 (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.4e-59
Identity = 137/413 (33.17%), Postives = 194/413 (46.97%), Query Frame = 0


Query: 4   QLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFD 63
           QLP++    + LK G+  WV  R  VR+ALE+YGCF A  D VSMEL  ++  +++ELF 
Sbjct: 7   QLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQGSVLKAMQELFA 66

Query: 64  VPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFCE 123
           +P + K +NV  KP+ GY   N L    ES GI+D  +L K   FT  +WP  GNK   +
Sbjct: 67  LPTEAKQRNVCPKPFTGYLSHNGL---SESFGIKDANILEKAHEFTQQLWP-EGNKSISK 126

Query: 124 NVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEM------ 183
            +  YA+ ++ LD  V+ ++ ESYG+    D H+ ST Y +R++KY     N++      
Sbjct: 127 MIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDITAAVGA 186

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 187 NVDNGANDNADGDANVNDDGASIGVKVNVDVGDDVNDNDSVNIGVGVDINVETNVNGDLD 246

Query: 244 ------------------------------NLGAFPHTDKSFLTILHQNQVNGLHIKTKD 303
                                          LG   HTDKS   I++Q+Q++GL +KTK+
Sbjct: 247 AEANGDATAWVVGAVSGNASVGAKEANVDAELGLPSHTDKSLTGIIYQHQIDGLEVKTKE 306

Query: 304 NKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNGRIYSPPHRV-IMSGKRERYSLGLFS--F 318
            KW+  +P     + ++ IV+AGDA  A  NGRI SP HRV +   K+ RY+  LFS   
Sbjct: 307 GKWIRVKP-----APNTVIVIAGDALCALMNGRIPSPYHRVRVTEKKKTRYAAALFSNPK 366

[Close]

BLAST of Spg036195 vs. ExPASy TrEMBL
Match: A0A6J1C712 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008991 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.2e-167
Identity = 283/325 (87.08%), Postives = 304/325 (93.54%), Query Frame = 0


Query: 4   QLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFD 63
           QLP+VKF  ENL    R+WV TREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELF+
Sbjct: 5   QLPLVKFSEENL----RSWVSTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFE 64

Query: 64  VPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFCE 123
           VPL+RKL+NVSEKPYHGYFGQNPLMPIHESMGIEDP LL+KTQSFTNLMWPSNGNK FCE
Sbjct: 65  VPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGNKSFCE 124

Query: 124 NVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNLGAFP 183
           NVNSYA+ ++ LD+TVK MVFESYGVR H +SH+KST+YLLRMIKYRVP+QNEMN+GAFP
Sbjct: 125 NVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMNMGAFP 184

Query: 184 HTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNGRIYS 243
           HTDKSFLTILHQNQVNGL IKTK NKWL+Y PS  SSSTSSFIVMAGDAFFAWSNGRIYS
Sbjct: 185 HTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPSSSSSSTSSFIVMAGDAFFAWSNGRIYS 244

Query: 244 PPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTERGQN 303
           PPH+VIMSGKRERYS+GLFSFN GIVQIPKELVD+ HPQQFKPFDHQDYLRFYSTERGQN
Sbjct: 245 PPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYSTERGQN 304

Query: 304 SPCAIKAYCGVGNGLLDTRGCILLH 329
           SPCAIKAYCG+GNGL+DTRGCILLH
Sbjct: 305 SPCAIKAYCGIGNGLVDTRGCILLH 325

[Close]

BLAST of Spg036195 vs. ExPASy TrEMBL
Match: A0A5D3BIZ7 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002910 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 6.9e-155
Identity = 269/331 (81.27%), Postives = 294/331 (88.82%), Query Frame = 0


Query: 1   MAGQLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKE 60
           M+ +LPVVKFCSENLKSGT+ WV T +KVREALE+YGCFVALYDSVS E+SSNMFDSLKE
Sbjct: 5   MSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMFDSLKE 64

Query: 61  LF-DVPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNK 120
           LF DVPL+RKLQNVSEKPYHGYFGQNPLMPIHESMGIE PIL T   SFTNLMWPS GN 
Sbjct: 65  LFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGND 124

Query: 121 RFCENVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNL 180
            F E+V +YAKLVS LD+ VK MVFESYGV N   SH++STKYL+RMIKYRVP++ EMNL
Sbjct: 125 SFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMNL 184

Query: 181 GAFPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQN+VNGL IKT+DNKW++Y P + SSSTSSFIVMAGDAFFAWSNG
Sbjct: 185 GAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHP-FSSSSTSSFIVMAGDAFFAWSNG 244

Query: 241 RIYSPPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTE 300
           RIYSPPHRVIMSG RERYS+GLFSFN GIVQIP+ELVDDKHPQ F PFDHQD+LRFYSTE
Sbjct: 245 RIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFYSTE 304

Query: 301 RGQNSPCAIKAYCGVGNGL--LDTRGCILLH 329
           RGQNSPCAIKAYCGVGN L  +DTR  +LLH
Sbjct: 305 RGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 334

[Close]

BLAST of Spg036195 vs. ExPASy TrEMBL
Match: A0A6J1EAT7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431473 PE=3 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 7.9e-151
Identity = 257/329 (78.12%), Postives = 289/329 (87.84%), Query Frame = 0


Query: 1   MAGQLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSS-NMFDSLK 60
           MAG++PVV+F S NL+SG R W  TREKVREALEEYGCFVALYDSVSME SS  M +++K
Sbjct: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 61  ELFDVPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNK 120
           E+F+VPL+RK+QNVSEKP+HGYFGQNPLMPIHESMGIEDP+L +  QSF+NLMWPS  N 
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 RFCENVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNL 180
            FCEN+ SYA+LVS L+QTVK MVFESYG+++ F SH +STKYLLRMIKYRVP +NEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQN VNGLHIKT+DN+W+EY+P    SS  SFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSPPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTE 300
           RIYS PHRV MSG RERYS+GLFSFN GIVQIPKELVDDKHPQQFK FDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 RGQNSPCAIKAYCGVGNGLLDTRGCILLH 329
           +GQ+SP AIKAYCGV NGLLD+R CI +H
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of Spg036195 vs. ExPASy TrEMBL
Match: A0A6J1E7C8 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431472 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 1.9e-149
Identity = 255/329 (77.51%), Postives = 288/329 (87.54%), Query Frame = 0


Query: 1   MAGQLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSS-NMFDSLK 60
           MAG+LPVV+F S NL+SG R W  TREKVREALEEYGCFVAL DSVSME SS  M ++++
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60

Query: 61  ELFDVPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNK 120
           E+F+VPL+RK+QNVSEKP+HGYFGQNPLMPIHESMGIEDP+L +  QSF+NLMWPS  N 
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 RFCENVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNL 180
            FCEN+ SYA+LVS L+QTVK MVFESYG+++ F SH +STKYLLRMIKYRVP +NEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQN VNGLHIKT+DN+W+EY+P    SS  SFI+MAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240

Query: 241 RIYSPPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTE 300
           RIYS PHRV MSG RERYS+GLFSFN GIVQIPKELVDDKHPQQFK FDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 RGQNSPCAIKAYCGVGNGLLDTRGCILLH 329
           +GQ+SP AIKAYCGV NGLLD+R CI +H
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of Spg036195 vs. ExPASy TrEMBL
Match: A0A6J1KFF7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111495326 PE=3 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.7e-148
Identity = 253/329 (76.90%), Postives = 287/329 (87.23%), Query Frame = 0


Query: 1   MAGQLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSS-NMFDSLK 60
           MAG++PVV+F S N++SG R W  TREKVREALEEYGCFVAL DSVSME SS  M +++K
Sbjct: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60

Query: 61  ELFDVPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNK 120
           E+F+VPL+RK+QNVS+KP+HGYFGQNPLMPIHESMGIEDP+L +  QSF+NLMWPS  N 
Sbjct: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 RFCENVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNL 180
            FC N+ SYA+LVS L+QTVK MVFESYG+++ F SH +STKYLLRMIKYRVP +NEMNL
Sbjct: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQN VNGLHIKT+DN+W+EY+P    SS  SFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240

Query: 241 RIYSPPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTE 300
           RIYS PHRV MSG RERYS+GLFSFN GIVQIPKELVDDKHPQQFK FDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 RGQNSPCAIKAYCGVGNGLLDTRGCILLH 329
           +GQ+SP AIKAYCGV NGLLD+R CI +H
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of Spg036195 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 330.9 bits (847), Expect = 1.2e-90
Identity = 155/314 (49.36%), Postives = 216/314 (68.79%), Query Frame = 0


Query: 1   MAGQLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVA-LYDSVSMELSSNMFDSLK 60
           M+ ++P + F  E+LK GT+ W  TRE +R+ALEEYGCF+  L D   ++L   +F SL 
Sbjct: 1   MSPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLV 60

Query: 61  ELFDVPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNK 120
           +LFD+P   K++N  +KP +GY GQ P +P+HES+GI++   L  T+SFT LMWP  GN+
Sbjct: 61  DLFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGNE 120

Query: 121 RFCENVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNL 180
            F E +  YA+  + LDQ V  MVF+SY V  ++D +++ST YLLR++K R P      L
Sbjct: 121 HFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTL 180

Query: 181 GAFPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNG 240
           G   HTDKSF TILHQ+QVNGL ++T++ + +        SS S F+V+AGDA  AWSN 
Sbjct: 181 GFVTHTDKSFTTILHQDQVNGLEMETREGERININ----LSSPSLFMVVAGDALMAWSND 240

Query: 241 RIYSPPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTE 300
           R++SP H+V++SG+ +RYSLG+F+FN G +Q+P+EL+D +HP  +KPFDH   LRFY T+
Sbjct: 241 RVWSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTD 300

Query: 301 RGQNSPCAIKAYCG 314
            G  S C IKAYCG
Sbjct: 301 TGYKSECPIKAYCG 309

[Close]

BLAST of Spg036195 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 324.7 bits (831), Expect = 8.5e-89
Identity = 153/313 (48.88%), Postives = 217/313 (69.33%), Query Frame = 0


Query: 4   QLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFD 63
           +LPV+ F ++NLK G   W LTR  V++AL++YG F A +D +  EL  ++F +L+ELFD
Sbjct: 11  RLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFD 70

Query: 64  VPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFCE 123
           +PL  KL+NVS+KP+HGY GQ P++P++ESMGI+D  +  K  +FT  +WP  GN  F  
Sbjct: 71  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFST 130

Query: 124 NVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNLGAFP 183
            + S++K +S LD T++ M+ ES+G+  + D H+ ST YLLR++KY+ P   E  +G   
Sbjct: 131 TIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNA 190

Query: 184 HTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNGRIYS 243
           HTDK+ +TIL+QN V GL ++TKD  W++ +P     +  SF VM GD+ +A  NGR++S
Sbjct: 191 HTDKNIVTILYQNHVEGLEVQTKDKNWIKVKP-----TQDSFTVMIGDSLYALLNGRLHS 250

Query: 244 PPHRVIMSGKRERYSLGLFSF-NAG-IVQIPKELVDDKHPQQFKPFDHQDYLRFYSTERG 303
           P HRV+M+G   RYSLGLFS   AG IV  P ELVD++HP+ FKPFDH ++L+FY TE G
Sbjct: 251 PYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAG 310

Query: 304 QNSPCAIKAYCGV 315
           Q S  A+K YCG+
Sbjct: 311 QRSQSALKTYCGI 317

[Close]

BLAST of Spg036195 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 315.1 bits (806), Expect = 6.7e-86
Identity = 148/313 (47.28%), Postives = 212/313 (67.73%), Query Frame = 0


Query: 4   QLPVVKFCS-ENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELF 63
           ++P++   S + LK  T  W     +  EALEEYGCF+A+YD V+ +L  ++F + +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 64  DVPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFC 123
           D+P + K +NV+EKPYHGY GQ P++P+HE +G++        Q FT+LMWP  GN RFC
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRFC 128

Query: 124 ENVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVPQQNEMNLGAF 183
             V++++  V+ LD+ V  M+FE+YGV  H++SHV S  YLL+ +KY  P ++ +++ AF
Sbjct: 129 NTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPES-ISMPAF 188

Query: 184 P-HTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNGRI 243
           P HTDK+FL+ILHQN VNGL +K+KD +W+  +         S++VMAGD    WSN RI
Sbjct: 189 PQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQ-----LPPKSYVVMAGDISMGWSNDRI 248

Query: 244 YSPPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYSTERG 303
            S  HRV M G + RY+LGLFSF   +V IP+ELVDDKHP  +KPFD+   + FY+T+ G
Sbjct: 249 RSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEG 308

Query: 304 QNSPCAIKAYCGV 315
           + +   +KAYCG+
Sbjct: 309 REANSTLKAYCGI 314

[Close]

BLAST of Spg036195 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 290.8 bits (743), Expect = 1.4e-78
Identity = 139/316 (43.99%), Postives = 206/316 (65.19%), Query Frame = 0


Query: 4   QLPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFD 63
           QLPV+ F  +NLK G+  W      V +ALE+YGCF A +D +S+EL+ ++F+++++LF+
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 64  VPLDRKLQNVSEKPYHGYFGQNPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFCE 123
           +P+  K +NVS KP+HGY   N    ++ES+GI D  +L K   FT  +WP +GNK   E
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 124 NVNSYAKLVSLLDQTVKNMVFESYGVRNHFDSHVKSTKYLLRMIKYRVP------QQNEM 183
            ++ +++ +  LD  V+ M+ ES+G+ N+ D H+ ST YL R++KY  P         E 
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEET 192

Query: 184 NLGAFPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWS 243
            LG   HTDK+ +TILHQ QV+GL +KTKD+KW++ +P     S  S +VM GD+  A  
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALL 252

Query: 244 NGRIYSPPHRVIMSGKRERYSLGLFSF--NAGIVQIPKELVDDKHPQQFKPFDHQDYLRF 303
           NGR++SP HRVIM+GK+ RYS GLFS      I+  P+ELVD +HP+ FKPF++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 304 YSTERGQNSPCAIKAY 312
           + TE G+ +  A+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 319

[Close]

BLAST of Spg036195 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 266.5 bits (680), Expect = 2.7e-71
Identity = 135/316 (42.72%), Postives = 194/316 (61.39%), Query Frame = 0


Query: 5   LPVVKFCSENLKSGTRNWVLTREKVREALEEYGCFVALYDSVSMELSSNMFDSLKELFDV 64
           LP++ F S+ L  GT +W+ TR+ VR A+E  G FVA +  VS +L  N+   +KE++ +
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKEMYYL 68

Query: 65  PLDRKLQNVSEKPYHGYFGQ-NPLMPIHESMGIEDPILLTKTQSFTNLMWPSNGNKRFCE 124
           P   K++N + K  HGY         IHES+GI+    L   + F+ L+WP  GN  FC+
Sbjct: 69  PDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP-QGNDPFCQ 128

Query: 125 NVNSYAKLVSLLDQTVKNMVFESYGV--RNHFDSHVKSTKYLLRMIKYRVPQQNEMNLGA 184
             + YA  ++ LDQTV  M++ESYG+  + H  SH +ST+YLLRM+ YR  Q  E N G 
Sbjct: 129 TTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTGF 188

Query: 185 FPHTDKSFLTILHQNQVNGLHIKTKDNKWLEYEPSYFSSSTSSFIVMAGDAFFAWSNGRI 244
             HTDKSF++ILHQN V GL +KT   +W+ + P     S + F+V++G    AWSN RI
Sbjct: 189 VSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNP-----SPTRFVVLSGMGLTAWSNDRI 248

Query: 245 YSPPHRVIMSGKRERYSLGLFSFNAGIVQIPKELVDDKHPQQFKPFDHQDYLRFYST--- 304
            +  H+V+MS    RYSLG FSF+ G ++ P+ELVDD+HP ++ PF+H   LRFY +   
Sbjct: 249 KACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYIN 308

Query: 305 ERGQNSPCAIKAYCGV 315
              ++S   ++ YCGV
Sbjct: 309 SLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:

BLAST of Spg036195 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137575.1	2.5e-167	87.08	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Momordica charant...	[more]
XP_038895877.1	1.5e-156	80.30	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida]	[more]
XP_038893580.1	1.3e-155	80.73	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida]	[more]
TYJ99087.1	1.4e-154	81.27	putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa]	[more]
XP_004151475.3	1.9e-154	82.32	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570...	[more]

BLAST of Spg036195 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	2.3e-78	44.13	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	1.9e-77	43.99	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q944Z5	1.2e-74	44.26	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
A0A0N9HQ36	6.8e-67	42.21	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q9ZTA1	2.4e-59	33.17	2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Spg036195 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C712	1.2e-167	87.08	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica chara...	[more]
A0A5D3BIZ7	6.9e-155	81.27	Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O...	[more]
A0A6J1EAT7	7.9e-151	78.12	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1E7C8	1.9e-149	77.51	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1KFF7	3.7e-148	76.90	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]

BLAST of Spg036195 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52790.1	1.2e-90	49.36	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	8.5e-89	48.88	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	6.7e-86	47.28	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	1.4e-78	43.99	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	2.7e-71	42.72	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 169..264 e-value: 1.2E-17 score: 64.3
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..302 e-value: 1.1E-52 score: 181.3
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 22..83 e-value: 2.8E-8 score: 34.5
None	No IPR available	PANTHER	PTHR47990:SF17	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKE	coord: 3..313
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 3..313
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 5..301
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 160..267 score: 10.586827

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg036195.1	Spg036195.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding