Spg036083 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg036083
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPolyphenol oxidase
Locationscaffold5: 41949122 .. 41954476 (+)
RNA-Seq ExpressionSpg036083
SyntenySpg036083
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAAAAGTTAAACCAAAGAGACCCTTATTCCAAGAATAATTTTATCCTCAGGCTTGCCTTGGGATCTTTGGCTATAAATATCAGCTGAAGAAGCAATACTTTTCAAACACAAGCTTAGATCTAATAATCAAAGCTAGCCTAATGGCTTCTCTATCTCCTCCTCCGCCTGCAACCCTTTCCTCCGCCACCGCCACCACTTATTTCGTCCCACTTTTCCGTAACAACAAATACCCATCATTCGGCCCTCGAAGCAGAAAGTTTCTCATCAACAAAGCTCCTCGAGTTTTGTGCAGCGCCTCCAATGGCGACCAAAAGTTCGACCGGAGAGACGTGCTCCTTGGGCTGGGCGGTCTCTACGGAGCCTCAACTCTCGGCCCGGATTCCTTCGCCTTCGCCAGTCCGGTGGTGACGCCGCCACTGGACAAGTGTCACTTAGCAACCTTGCCAGATGGCACAAAGGATGCTTGCTGCCCGCCGACGGCCGTCAACATCAAAGACTTCCGGCCAACCCCACCTAGCAAACTGCGGGTTAGGCCGGCGGCCCATTTAATTGATGAAAAGTACATAGAGAAATACAAGAGAGCCGTCCAGCTGATGAAGTGCTTGCCGGACAACGACCCAAGAAATTTTAGACAGCAAGCGAATGTTCACTGTGCCTATTGTCATGGCGGCTACGACCAAGTGGGGTTTCCAGTGCAGGTCCAAGTTCATAACTCGTGGCTGTTTTTCCCATTCCATCGTTACTATCTCTACTTTTACGAGAAGATCTTGGGAGAGCTGATCGGTGATCCCACTTTCGGGTTGCCGTTTTGGAACTACGACGCTCCGAAGGGCATGAGAATGCCGGCCATCTTCGCCGACAAGAACTCGCCGCTTTACGATCAAGTTCGTAATCTAAATCATCTGCCGCCGGCGTTAGTGGATCTGAATCTGGATGACGCGGAAACAGACGAAGAAACTCTAGTAAAGAGAAACCTCCGGGTTATGTACCGGCAGGTGGTGTCCGGCGCCCGTACGCCGTCTCTGTTTTTTGGCGTCCCTTATCGAGGGGGTGATGCAGCACCAGAACAAGGTGTTTTTCATAACTACATATTTTCAGACTTTTTACTTTCAATAATACATTTTCCTCCTTTTTTGGTCTCTACATTTTTTTTCAAATAATGCTTTTCTATTCTTCAAATTTTCAAAATGTTTATTTTGGTTTCAACTTCTTAAAATTTGTCGTAGTCTTTGCTTTGATACTTCAAAATGTCTGTTGTAATTTTTAGATTTGAGAAAGTTTATTGATTTTATATTGTAGGATTTTTTTTTATTAATATTATAAATTTTTAAACTTATATTAGAGTAATAAGGAAAATTATTATTCTAATATTGTGGTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGTTGTATAAGTTGTCTTTGTCGTCGTCTCTTAACTTGCACACAAATGTTTCATTAATTTATATTTGGCACAATGGCAAAAGATGCCTTTGACATTGAGGAGAACGTGGTTCAAATCTTGTCCAACGTGGAACTTTATCTTTTGCTCATTTTTCCTCTCTCTTTTTTCCTCTTCTTTTTTTTTTTTTTTTTCTTTTCCTTTCATTTGCTTTTCAAAAAATTGAAATAATATGCTATTTTTTTCTCTTTTATTTTTTTAAAAATCAAATAAAGTAATTTTCCTCTTTTTAAATCAAATAAATTAGTTATATTTTTTTGTTTAATGTTTTAAACACATTAAATATTATATTGCGATTTATTATCTTTTGTTAAAGAAATTAAAATTTAATTAAGTGTTAATTATTATAATTTTTCCTTTTTCACAACCTTATTTGATTTTTTAAAAAAAAATAAAAGAGAAAAAAATAGCAGGTTATTTCAATTTTTTGAAAAGGAAATGAAAGGAAAAGAAAAAAAAATAAAAAATGGAAGATAAAAAAGAGAGAGGAAAAATGAGGTGAAAAAAAAAAAGGAGAAAAAGACAAAGTTTCACGTTTGGCAATATTTGAACTCTCGTTCTCCTATTCTCCTAGATGTTAAAAGCGTCCTTTGCCATTATGCCAAATACAAATTAATATAACTTTAGAGTGCAGCTACCTCCTTTGCTGTTGTTTGTTTTGTAATTTCCATTTGTTATTTCAATTTTTTTTTGTAATTTCCGTTCTTTTGTAGCTAATGGCTTCCCTTTCTCCTCCTCCGCCTGCAACCTACTTTACAGTGAGAGAAGGAAAAAAATTAAGCATTTTCCAATAAGGTATTTTTGAATTTTTTTTTTAAAGTTTAAGGTATTTTTGAAACTGAAACCTTAACGGTTTCCATCCAAAAATTAACAGCAAGAGTATTTTTGAAACATTTTTCAAAGTTTAAGGGTATTTTTGAAACTTTTCTAAGTTCAGGGACATTTTTTAAACAAACCCCATAGTTCAGGGGTATTTTTTATAATTTACTCAAAGTAGATATGTTGAAAGTTTATTAAAAAAAAAAAAGTATGGTGAAAGTTTTAAGGTCAAAAGAATTAATTTTAGAAGCTTACACATCAAAATTAAAAAAGTCTTTACATGTGTCTATGATATAAATAAGATGGAGTTGTGTTAATAGCTTTAAGTTTACCTCGTTTGAGTTCATTTAGGGCCCGTTTGGTAACGTTCTCGTTTCTTGTTTCCGTTTCTTCATTTTTAATAAAGAAAAATAGAATTTCATGACCCTAACACCTATACATTCAATAATAATAAACCTATATTTTTATTTATGTTTGGAAAAAATTGGAAACAAGAACAAAAAAGGAGAAACTAGTTTTAGTTGTTCCCAAAAGTTCTATACAAATTTTGGAAGTGTTTCTAAAAAATGAGAAAGAAGAAACATACATAGATATTAAATGCTTATATTTCTTAATAAAATTAGAAACAAGAAACGAGAAATGAGAACGTTACCAAACAGGCCCTTAGATTTTGGTTTTTGAAAATTATGTTTGTTTTTTCTTAAATTCTTTAACATGATTTTCATCTTTGTTAACGATACATTGGAATTCTTAGTCAAATTCCAAAAACAAAAACAAGTCTTTGAAAACTATTTTTTTTTTCTTTCAAAATTTGAGTTGGTTTTTGAAAATATAGAAAGAGGATAAGTACAACACAAAAAACTTAGAGAAGTAGTTTTTGTAGGCTTAATTTTCAAAAATAAAAAACAAAAAACAAAATGATTATTAAAATGTTTGAATTTTCATTTGGTAGAAACAAAAAATCTCTAATAATTTCTCGTGAGTTTAAACTGTTTAGAAATTAAATCAAGAACCATGTAACACCATTGTCTTGGGTTCAATTTCTAAAGTTTCAAAATTGTGTCGGATAGGTTGTCAATAAATAAATAAATAAATACACCGCGTCTCATAGGATAAAAATTCAAAATAGATTATTAAGGCCTTGCTAACCATTTGGTTTTTTTTTAATTTTTTTAAAACTAAGCTTAATTATGAATACTACTACTATACCTATTAGTTTTTTTTTTTTGATAAGTATTTTTAAAATTCAAATCAAATTTGGGAAACTAAAAACAGTAATTTTTATTAATATTATTATGGTCTTGGAATTTGACTAAAAATCAAAATGTTTCTTTTAGAAATCATAGAAAATATACTTAATTTTCAAAAACTAAAAACCTATCCAATAGAACCAAAACTTTTAGGACCTGTTTGATAACCATTTGGTTTTTTGTTTTTTGGTTTTTCAAAATTATGCTTGTTTTCTCCTAAATTCACTACCATGATTTTCATCCTAGTTAAGGTTGCATTTGAATTCCTAACCAAAATTTAAAAACAAAAACAAGTTTTTGAAAATTATTTTTTCGTGTTTCCAAAATTGGACTTGGTTTTTAGAAATAAGGTAGAAGATTAATACTAAAATCATAAAACTTATTGGTGCAAAAAACCAAAAACCAAAAACCAAATGATTATCAAACGGGACCTTAATTTTGGTTTGATAGATTCCTCAATGTCTAATATAGATCTATAAAGATTTTCGAAAAACAAATAGAAAAATTAATATAAAAACCACAAAAAGCATCATGACTCTTTGTCCTCTCCAACACAAATGACCTAATTTCTTTTCCCTTTTAATAGGCAAAAAGTTTTTTTTAGTACATCAACATTTGGGATAGGGGAATTCGAACCTCTGACGTCTTGGTGGAGGGTACATGCCAATTACTGTTGAACTAAGCTCGCTTTAATTTAATAGGCAAAAAGTTACAAATGCATAATTTTGATTTAATTTAATATACATGTACACAACTTGCACAGGAGGAGGATCTGTGGAAAACGTTCCTCACACCCCGATCCATATTTGGTGCGGCGATAGCGACCAAGGAGGTAGGGACATGGGAGCTTTCTACTCCGCCGCTCGTGACCCAATCTTCTACGCCCACCACTCCAACGTCGACCGCCTTTGGTCCATCTGGAAGACCCTCGGCGGCAAGCGCGAAGACCTCAAAGACCCTGATTATCTCAACTCTTCTTTCATATTCTACAACGAGAAGGCCGAAGCCGTCCGAGTCTACGTCCGCGACTGTCTCGACCTCAAAAACCTCGGCTACGTCTACCAGGACGTCGACATTCCATGGATCAAGACCCGCCCTACCAAACGCAACAAGCCCAAGAAGAAGCCCAAGAAACAAGTGGCCTCCACCGTCCCCTTCGGCGTCGGCGCAGCCCTCGCCGAGCCGATCTCGTTCACCGTCGAGAAGGCGGGCATGGCCCAGTTCCCCTTGACTCTGGACAAGATCGTCAGGACGGAAGTGAAGAGGCCGAAGAAGTCGAGGAGCAAGGAGGAGAAAGAGGAGGAAGAGGAGATTCTGCTGATCGAAGGAATCGAAGTCGACGCCGGCAAGTCGTCAAAGTTCGACGTCTATATCAACGACGAGGACGACAGGGAAATTGGACCTGATAATACTGAGTTTGCTGGGAGCTTTGTGAATTTGCCTCATAAGGTGAGCGGCAAAAATACGACGACCTTGAAGACCTCCCTTAGGCTGGGGCTCAACGAACTGCTTGAAGATTTGGAGGTGGATGATGATGAAAGCATCGTGGTCACTCTGGTGCCCAAGTTTGGATTCGACCAATCGCCCATCATCATTGGTAACATCAAGATTGAATTACTAATAGAATGAGTATTCCTATTTTTGGGATCGTTAAGTTATGTGTGTTAATAATGTCCACCCAGTTATGTCTTGTGTGGCCTTTCTTTGTTACCAATTTGGATTCGACGTTTGAAAATCTATGTACTACTCCTACTTTTGGGAGGGACTATCTTTATAATTCAATGAAATTTCGATGATGCTGATGTTAAAT

mRNA sequence

ATGGCTTCTCTATCTCCTCCTCCGCCTGCAACCCTTTCCTCCGCCACCGCCACCACTTATTTCGTCCCACTTTTCCGTAACAACAAATACCCATCATTCGGCCCTCGAAGCAGAAAGTTTCTCATCAACAAAGCTCCTCGAGTTTTGTGCAGCGCCTCCAATGGCGACCAAAAGTTCGACCGGAGAGACGTGCTCCTTGGGCTGGGCGGTCTCTACGGAGCCTCAACTCTCGGCCCGGATTCCTTCGCCTTCGCCAGTCCGGTGGTGACGCCGCCACTGGACAAGTGTCACTTAGCAACCTTGCCAGATGGCACAAAGGATGCTTGCTGCCCGCCGACGGCCGTCAACATCAAAGACTTCCGGCCAACCCCACCTAGCAAACTGCGGGTTAGGCCGGCGGCCCATTTAATTGATGAAAAGTACATAGAGAAATACAAGAGAGCCGTCCAGCTGATGAAGTGCTTGCCGGACAACGACCCAAGAAATTTTAGACAGCAAGCGAATGTTCACTGTGCCTATTGTCATGGCGGCTACGACCAAGTGGGGTTTCCAGTGCAGGTCCAAGTTCATAACTCGTGGCTGTTTTTCCCATTCCATCGTTACTATCTCTACTTTTACGAGAAGATCTTGGGAGAGCTGATCGGTGATCCCACTTTCGGGTTGCCGTTTTGGAACTACGACGCTCCGAAGGGCATGAGAATGCCGGCCATCTTCGCCGACAAGAACTCGCCGCTTTACGATCAAGTTCGTAATCTAAATCATCTGCCGCCGGCGTTAGTGGATCTGAATCTGGATGACGCGGAAACAGACGAAGAAACTCTAGTAAAGAGAAACCTCCGGGTTATGTACCGGCAGGTGGTGTCCGGCGCCCGTACGCCGTCTCTGTTTTTTGGCGTCCCTTATCGAGGGGGTGATGCAGCACCAGAACAAGGAGGAGGATCTGTGGAAAACGTTCCTCACACCCCGATCCATATTTGGTGCGGCGATAGCGACCAAGGAGGTAGGGACATGGGAGCTTTCTACTCCGCCGCTCGTGACCCAATCTTCTACGCCCACCACTCCAACGTCGACCGCCTTTGGTCCATCTGGAAGACCCTCGGCGGCAAGCGCGAAGACCTCAAAGACCCTGATTATCTCAACTCTTCTTTCATATTCTACAACGAGAAGGCCGAAGCCGTCCGAGTCTACGTCCGCGACTGTCTCGACCTCAAAAACCTCGGCTACGTCTACCAGGACGTCGACATTCCATGGATCAAGACCCGCCCTACCAAACGCAACAAGCCCAAGAAGAAGCCCAAGAAACAAGTGGCCTCCACCGTCCCCTTCGGCGTCGGCGCAGCCCTCGCCGAGCCGATCTCGTTCACCGTCGAGAAGGCGGGCATGGCCCAGTTCCCCTTGACTCTGGACAAGATCGTCAGGACGGAAGTGAAGAGGCCGAAGAAGTCGAGGAGCAAGGAGGAGAAAGAGGAGGAAGAGGAGATTCTGCTGATCGAAGGAATCGAAGTCGACGCCGGCAAGTCGTCAAAGTTCGACGTCTATATCAACGACGAGGACGACAGGGAAATTGGACCTGATAATACTGAGTTTGCTGGGAGCTTTGTGAATTTGCCTCATAAGGTGAGCGGCAAAAATACGACGACCTTGAAGACCTCCCTTAGGCTGGGGCTCAACGAACTGCTTGAAGATTTGGAGGTGGATGATGATGAAAGCATCGTGGTCACTCTGGTGCCCAAGTTTGGATTCGACCAATCGCCCATCATCATTGGTAACATCAAGATTGAATTACTAATAGAATGA

Coding sequence (CDS)

ATGGCTTCTCTATCTCCTCCTCCGCCTGCAACCCTTTCCTCCGCCACCGCCACCACTTATTTCGTCCCACTTTTCCGTAACAACAAATACCCATCATTCGGCCCTCGAAGCAGAAAGTTTCTCATCAACAAAGCTCCTCGAGTTTTGTGCAGCGCCTCCAATGGCGACCAAAAGTTCGACCGGAGAGACGTGCTCCTTGGGCTGGGCGGTCTCTACGGAGCCTCAACTCTCGGCCCGGATTCCTTCGCCTTCGCCAGTCCGGTGGTGACGCCGCCACTGGACAAGTGTCACTTAGCAACCTTGCCAGATGGCACAAAGGATGCTTGCTGCCCGCCGACGGCCGTCAACATCAAAGACTTCCGGCCAACCCCACCTAGCAAACTGCGGGTTAGGCCGGCGGCCCATTTAATTGATGAAAAGTACATAGAGAAATACAAGAGAGCCGTCCAGCTGATGAAGTGCTTGCCGGACAACGACCCAAGAAATTTTAGACAGCAAGCGAATGTTCACTGTGCCTATTGTCATGGCGGCTACGACCAAGTGGGGTTTCCAGTGCAGGTCCAAGTTCATAACTCGTGGCTGTTTTTCCCATTCCATCGTTACTATCTCTACTTTTACGAGAAGATCTTGGGAGAGCTGATCGGTGATCCCACTTTCGGGTTGCCGTTTTGGAACTACGACGCTCCGAAGGGCATGAGAATGCCGGCCATCTTCGCCGACAAGAACTCGCCGCTTTACGATCAAGTTCGTAATCTAAATCATCTGCCGCCGGCGTTAGTGGATCTGAATCTGGATGACGCGGAAACAGACGAAGAAACTCTAGTAAAGAGAAACCTCCGGGTTATGTACCGGCAGGTGGTGTCCGGCGCCCGTACGCCGTCTCTGTTTTTTGGCGTCCCTTATCGAGGGGGTGATGCAGCACCAGAACAAGGAGGAGGATCTGTGGAAAACGTTCCTCACACCCCGATCCATATTTGGTGCGGCGATAGCGACCAAGGAGGTAGGGACATGGGAGCTTTCTACTCCGCCGCTCGTGACCCAATCTTCTACGCCCACCACTCCAACGTCGACCGCCTTTGGTCCATCTGGAAGACCCTCGGCGGCAAGCGCGAAGACCTCAAAGACCCTGATTATCTCAACTCTTCTTTCATATTCTACAACGAGAAGGCCGAAGCCGTCCGAGTCTACGTCCGCGACTGTCTCGACCTCAAAAACCTCGGCTACGTCTACCAGGACGTCGACATTCCATGGATCAAGACCCGCCCTACCAAACGCAACAAGCCCAAGAAGAAGCCCAAGAAACAAGTGGCCTCCACCGTCCCCTTCGGCGTCGGCGCAGCCCTCGCCGAGCCGATCTCGTTCACCGTCGAGAAGGCGGGCATGGCCCAGTTCCCCTTGACTCTGGACAAGATCGTCAGGACGGAAGTGAAGAGGCCGAAGAAGTCGAGGAGCAAGGAGGAGAAAGAGGAGGAAGAGGAGATTCTGCTGATCGAAGGAATCGAAGTCGACGCCGGCAAGTCGTCAAAGTTCGACGTCTATATCAACGACGAGGACGACAGGGAAATTGGACCTGATAATACTGAGTTTGCTGGGAGCTTTGTGAATTTGCCTCATAAGGTGAGCGGCAAAAATACGACGACCTTGAAGACCTCCCTTAGGCTGGGGCTCAACGAACTGCTTGAAGATTTGGAGGTGGATGATGATGAAAGCATCGTGGTCACTCTGGTGCCCAAGTTTGGATTCGACCAATCGCCCATCATCATTGGTAACATCAAGATTGAATTACTAATAGAATGA

Protein sequence

MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQKFDRRDVLLGLGGLYGASTLGPDSFAFASPVVTPPLDKCHLATLPDGTKDACCPPTAVNIKDFRPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCHGGYDQVGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRMPAIFADKNSPLYDQVRNLNHLPPALVDLNLDDAETDEETLVKRNLRVMYRQVVSGARTPSLFFGVPYRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGGRDMGAFYSAARDPIFYAHHSNVDRLWSIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGYVYQDVDIPWIKTRPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLTLDKIVRTEVKRPKKSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTEFAGSFVNLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFGFDQSPIIIGNIKIELLIE
Homology
BLAST of Spg036083 vs. NCBI nr
Match: AKN08990.1 (polyphenol oxidase [Luffa aegyptiaca])

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 573/598 (95.82%), Postives = 576/598 (96.32%), Query Frame = 0

Query: 1   MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQKFD 60
           MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQKFD
Sbjct: 1   MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQKFD 60

Query: 61  RRDVLLGLGGLYGASTLGPDSFAFASPVVTPPLDKCHLATLPDGTKDACCPPTAVNIKDF 120
           RRDVLLGLGGLYGASTLGPDSFAFASPVVTPPLDKCHLATLPDGTKDACCPPTAVNIKDF
Sbjct: 61  RRDVLLGLGGLYGASTLGPDSFAFASPVVTPPLDKCHLATLPDGTKDACCPPTAVNIKDF 120

Query: 121 RPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCHGGYDQ 180
           RPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCHGGYDQ
Sbjct: 121 RPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCHGGYDQ 180

Query: 181 VGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRMPAIFAD 240
           VGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRMPAIFAD
Sbjct: 181 VGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRMPAIFAD 240

Query: 241 KNSPLYDQVRNLNHLPPALVDLNLDDAETDEETLVKRNLRVMYRQVVSGARTPSLFFGVP 300
           KNSPLYDQVRNLNHLPPALVDLNLDDAETDEETLVKRNLRVMYRQVVSGARTPSLFFGVP
Sbjct: 241 KNSPLYDQVRNLNHLPPALVDLNLDDAETDEETLVKRNLRVMYRQVVSGARTPSLFFGVP 300

Query: 301 YRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGGRDMGAFYSAARDPIFYAHHSNVDRLW 360
           YRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGGRDMGAFYSAARDPIFYAHHSNVDRLW
Sbjct: 301 YRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGGRDMGAFYSAARDPIFYAHHSNVDRLW 360

Query: 361 SIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGYVYQDVDIPWIKT 420
           SIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGYVYQD DIPWIKT
Sbjct: 361 SIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGYVYQDADIPWIKT 420

Query: 421 RPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLTLDKIVRTEVKRPK 480
           RPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLTLDKIVRTEVKRPK
Sbjct: 421 RPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLTLDKIVRTEVKRPK 480

Query: 481 KSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTEFAGSFVNLPHKV 540
           KSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTEFAGSFVNLPHKV
Sbjct: 481 KSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTEFAGSFVNLPHKV 540

Query: 541 SGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFGFDQSPIIIGNIKIELLIE 599
           SGKNTTTLKTSL  G    L+       ++      P  GFDQSPIIIGNIKIELLIE
Sbjct: 541 SGKNTTTLKTSLSWGSTNCLKIWRWMMMKASWSLWCPSLGFDQSPIIIGNIKIELLIE 598

BLAST of Spg036083 vs. NCBI nr
Match: APO14262.1 (polyphenol oxidase 3 [Luffa aegyptiaca])

HSP 1 Score: 746.5 bits (1926), Expect = 1.8e-211
Identity = 378/600 (63.00%), Postives = 459/600 (76.50%), Query Frame = 0

Query: 4   LSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQK-FDRR 63
           ++  PPA LSSA  TT F P   NNK P    R  K+  +KAPR+LCSASNG Q   DRR
Sbjct: 1   MATSPPA-LSSAATTTTFGPFLPNNKNPPPNLRRTKYFSSKAPRILCSASNGGQNHIDRR 60

Query: 64  DVLLGLGGLYGASTLGPDSFAFASPVVTPPLDKCHLATLPDGTKDA-CCPPTAVNIKDFR 123
           D+L+GLGGLYGASTL   S AFA+PV TP + KC    LP G     CCPP    + DFR
Sbjct: 61  DILIGLGGLYGASTLSQGSLAFAAPVQTPNISKCGQPDLPPGADPINCCPPPTTAVVDFR 120

Query: 124 PTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCHGGYDQV 183
           P  P KLRVRPAAHL D+ Y+EKYKRAV+LMK LPD+DPR+F+QQANVHCAYC+G YDQV
Sbjct: 121 PQAPGKLRVRPAAHLADQNYVEKYKRAVELMKALPDDDPRSFKQQANVHCAYCNGAYDQV 180

Query: 184 GFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRMPAIFADK 243
           GFPV+VQVHNSWLFFPFHRYYL+F+EKILGELIGDP+F LPFWNYDAP GM+MPA++AD+
Sbjct: 181 GFPVEVQVHNSWLFFPFHRYYLHFHEKILGELIGDPSFALPFWNYDAPGGMKMPAMYADE 240

Query: 244 NSPLYDQVRNLNHLPPALVDLNLD--DAETDEETLVKRNLRVMYRQVVSGARTPSLFFGV 303
            S LYD++RN NHLPP LVDL+    D    +E  ++ NL +MYRQ+VS +RTPSLFFG 
Sbjct: 241 KSSLYDELRNRNHLPPTLVDLDFGGVDPTIGDEAQIRSNLSIMYRQMVSNSRTPSLFFGN 300

Query: 304 PYRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGG-RDMGAFYSAARDPIFYAHHSNVDR 363
            Y  GD  P  GGG VEN+PH P+HIWCGD+++    +MG FYSAARDPIFYAHHSN+DR
Sbjct: 301 AYLAGD-EPSPGGGLVENIPHGPVHIWCGDNNEPNFENMGNFYSAARDPIFYAHHSNIDR 360

Query: 364 LWSIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGYVYQDVDIPWI 423
           LWS+WKTLGG+R+DL++ D+LN+SFIFY+EKA+AVRV VRDCLD +NLGYVYQDV +PW+
Sbjct: 361 LWSVWKTLGGRRQDLQNADWLNASFIFYDEKAQAVRVRVRDCLDSRNLGYVYQDVQLPWL 420

Query: 424 KTRPTKRNKPKKKPKKQVASTVPFGVGAALAE----PISFTVEKAGMAQFPLTLDKIVRT 483
           ++RPT R     + +K VA  +PFGVG ALA     P S  +  A + +FP+TL+  +  
Sbjct: 421 ESRPTPRR--ALRAQKAVAH-LPFGVGPALAAGEPGPSSSRLRPAKI-KFPITLNSPISM 480

Query: 484 EVKRPKKSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTEFAGSFV 543
           EVKRPK+SRSK+EK+EEEEI++I GIE DA K+ KFDV+INDEDD+EI PDNTEFAGSFV
Sbjct: 481 EVKRPKRSRSKKEKDEEEEIIVIYGIEFDANKAIKFDVFINDEDDKEIRPDNTEFAGSFV 540

Query: 544 NLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFGFDQSPIIIGNIKIE 595
           N+PHK   K    +KT LRLG+ ELLEDL+ DDD+S+ VTLVP+ G  +  + I  IKIE
Sbjct: 541 NVPHKHGHKK--NVKTGLRLGITELLEDLDADDDDSVRVTLVPRSG--KGLVSIAGIKIE 590

BLAST of Spg036083 vs. NCBI nr
Match: XP_038903169.1 (polyphenol oxidase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 708.0 bits (1826), Expect = 7.0e-200
Identity = 359/592 (60.64%), Postives = 440/592 (74.32%), Query Frame = 0

Query: 1   MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVL-CSASN-GDQ- 60
           MASLS PP       + TTYF          SF P+++    N +PR++ C+  N  DQ 
Sbjct: 1   MASLSHPP-------SLTTYF------PSSNSFLPKTKTLPNNISPRIISCNTVNHNDQN 60

Query: 61  ----KFDRRDVLLGLGGLYGASTLGPDS-FAFASPVVTPPLDKCHLATLPDGTKDA-CCP 120
               K DRRD+L+GL GLYGASTL  +S FA A+P+ TP + KC  A LP G     CCP
Sbjct: 61  TPKPKIDRRDILVGLSGLYGASTLYKNSNFALAAPIQTPNISKCGPADLPPGADSTNCCP 120

Query: 121 PTAVNIKDFRPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHC 180
           P A  I DF+P    KLRVRPAAHL+D+ YIEKYK+AV+LMK LP+NDPR+F QQANVHC
Sbjct: 121 PPATRILDFQPPAQEKLRVRPAAHLVDKNYIEKYKKAVELMKALPENDPRSFTQQANVHC 180

Query: 181 AYCHGGYDQVGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKG 240
           AYC+G YDQVGFPV+VQVHNSWLFFPFHR YLYF+EKILGELIGDP F LPFWNYDAP G
Sbjct: 181 AYCNGAYDQVGFPVEVQVHNSWLFFPFHRLYLYFHEKILGELIGDPNFALPFWNYDAPGG 240

Query: 241 MRMPAIFADKNSPLYDQVRNLNHLPPALVDLNLD--DAETDEETLVKRNLRVMYRQVVSG 300
           MRMP ++A++ S LYD++RN NH+PP LVDL+    D    ++  ++ NL +MYRQ+VS 
Sbjct: 241 MRMPEMYAEEKSSLYDELRNENHVPPRLVDLDFGRVDPNIGDDAQIRSNLSIMYRQMVSN 300

Query: 301 ARTPSLFFGVPYRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQ-GGRDMGAFYSAARDPI 360
           +RTPSLFFG  YR GD  P  GGGS+EN+PH P+H+WCGD+++  G +MG FYSAARDPI
Sbjct: 301 SRTPSLFFGSAYRAGD-EPSPGGGSIENIPHGPVHVWCGDNNEPNGENMGNFYSAARDPI 360

Query: 361 FYAHHSNVDRLWSIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGY 420
           FYAHHSN+DRLWS+WKTLGG+R+DL++P++LN+SFIFYNEKAEAVRV VR  LD K LGY
Sbjct: 361 FYAHHSNIDRLWSVWKTLGGRRQDLQNPEWLNTSFIFYNEKAEAVRVRVRQSLDSKILGY 420

Query: 421 VYQDVDIPWIKTRPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLTL 480
           VYQDVD+PW++TRPT R   K      +   +PF +G ALA   S +  +    +FP+T+
Sbjct: 421 VYQDVDVPWLQTRPTPRRAQKTVANNNI--ILPFSIGPALAAGPSSSRVRPANIKFPITV 480

Query: 481 DKIVRTEVKRPKKSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTE 540
              V  EVKRPKKSRSK+EKEEEEEIL+I GI  D  +  KFDV+INDEDD+EI PDNTE
Sbjct: 481 KSAVSVEVKRPKKSRSKKEKEEEEEILVIYGIGFDPNQGIKFDVFINDEDDKEIRPDNTE 540

Query: 541 FAGSFVNLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFG 581
           FAGSFVN+PHK+  K   T+KT LRLG+ E+LEDLE DDD+S+ VT+VP+ G
Sbjct: 541 FAGSFVNVPHKLGHKK--TVKTGLRLGITEILEDLEADDDDSVRVTIVPRLG 574

BLAST of Spg036083 vs. NCBI nr
Match: XP_023001713.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 707.6 bits (1825), Expect = 9.1e-200
Identity = 371/601 (61.73%), Postives = 447/601 (74.38%), Query Frame = 0

Query: 1   MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQKFD 60
           MASL  PPPA LS+AT        FR    PS  P + K+L N A RVLCSA+NG  K D
Sbjct: 1   MASL--PPPA-LSTAT--------FR----PSSRPPTSKYLPNNAARVLCSAANG--KID 60

Query: 61  RRDVLLGL--GGLYGASTLGPDS-FAFASPVVTPPLDKCHLATLPDGTKDA-CCPPTAVN 120
           RRDVL+G+  GG+YGAS    DS FA A+PV TP + KC    LP G+    CCPP + +
Sbjct: 61  RRDVLIGIGAGGIYGASAFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPS 120

Query: 121 IKDFRPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCHG 180
           I DFRP  P KLRVRPAAHL D+ YI+KYKRAV+LMK LP +DPR+F QQANVHCAYC+G
Sbjct: 121 IVDFRPPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNG 180

Query: 181 GYDQVGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRMPA 240
           GYDQVGFPV+VQVHNSWLFFPFHRYYLYFYEKILGELIGDPTF LPFWNYDAP GMRMPA
Sbjct: 181 GYDQVGFPVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPA 240

Query: 241 IFADKNSPLYDQVRNLNHLPPALVDLNLD--DAETDEETLVKRNLRVMYRQVVSGARTPS 300
           ++AD NS LYDQ+RN NHLPP L+DL+ D  D+   +   ++ NLR+MYRQ+VS +RTP 
Sbjct: 241 MYADHNSSLYDQLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPK 300

Query: 301 LFFGVPYRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGG-RDMGAFYSAARDPIFYAHH 360
           LFFG  Y  GD  P  GGGSVEN+PH  +H WCGD+++    +MG FYSAARDPIFY+HH
Sbjct: 301 LFFGSAYVAGD-EPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHH 360

Query: 361 SNVDRLWSIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGYVYQDV 420
           SNVDR+WS+WKTLGG+R DL+ P++L++SFIFYNEKA+AVRV VRDCLD KNLGY+YQDV
Sbjct: 361 SNVDRMWSVWKTLGGRRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDV 420

Query: 421 DIPWIKTRPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLTLDKIVR 480
           ++PW++TRPT R     + +K V S VP           S  V+   M + P+TL   + 
Sbjct: 421 ELPWLETRPTPRR--ALRAQKSVDSNVP-----------SRRVKPPKM-KLPVTLKSAIS 480

Query: 481 TEVKRPKKSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTEFAGSF 540
           TEVKR KKSR ++EK+EEEEIL+I+GIE D   S K D+YINDEDDREI  DNTEFAGSF
Sbjct: 481 TEVKRGKKSRGRKEKDEEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAGSF 540

Query: 541 VNLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFGFDQSPIIIGNIKI 595
           VN+PHK   K    +KT LRLG++E+LEDL+ DDD+SI+VT++P+ G     + I  IKI
Sbjct: 541 VNVPHKHGHKK--KVKTGLRLGISEVLEDLDADDDDSIIVTIIPRLG--AGLVSIAEIKI 565

BLAST of Spg036083 vs. NCBI nr
Match: XP_022139808.1 (polyphenol oxidase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 704.9 bits (1818), Expect = 5.9e-199
Identity = 370/614 (60.26%), Postives = 445/614 (72.48%), Query Frame = 0

Query: 1   MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAP---RVLCSASNGDQ 60
           MAS+ PP P +L         +P FR           RKF++NK     RV+CSASN   
Sbjct: 9   MASVCPPLPPSL---------LPSFR-----------RKFVVNKNNKHCRVVCSASNKHD 68

Query: 61  K--------FDRRDVLLGLGGLYGASTLGP--DSFAFASPVVTPPLDKCHLATLPDGTKD 120
                     DRRDVL+GLGGLYGAS+  P  DSFA A+PV TP + KC  A + D  K 
Sbjct: 69  SQSQSPGNMVDRRDVLIGLGGLYGASSFAPEXDSFAIAAPVDTPQITKCGPAQVRD-KKV 128

Query: 121 ACCPPTAVNIKDFRPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQA 180
            CCPP ++ IKDFRPTPP KLRVRPAAHL+D+ Y++KY+RAV+LM+ LPD+DPR+F QQA
Sbjct: 129 ECCPPPSIKIKDFRPTPPRKLRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQA 188

Query: 181 NVHCAYCHGGYDQVGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYD 240
           N+HCAYC+GGY Q+GFPV++QVHNSWLFFPFHR+YLYF+EKILGELIGDPTF LPFWNYD
Sbjct: 189 NIHCAYCNGGYKQLGFPVELQVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYD 248

Query: 241 APKGMRMPAIFADKNSPLYDQVRNLNHLPPALVDL--NLDDAETDEETLVKRNLRVMYRQ 300
           AP GM  PAIFA++NSPLYD+ RN NH+PP +VDL  N  D   D+E  V+ NLRVMYRQ
Sbjct: 249 APAGMTFPAIFANQNSPLYDEFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQ 308

Query: 301 VVSGARTPSLFFGVPYRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQ-GGRDMGAFYSAA 360
           +VSG+RT SLFFG PYR GD  P  G GSVENVPH PIH WCG+  Q  G DMG FYSA 
Sbjct: 309 MVSGSRTRSLFFGRPYRAGD-DPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAG 368

Query: 361 RDPIFYAHHSNVDRLWSIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLK 420
           RDP+FY+HH+NVDR+W IWKTLG +R D+++PD+LN+SF+FYNEK EAVRV VRDCLD  
Sbjct: 369 RDPVFYSHHANVDRMWCIWKTLGRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTT 428

Query: 421 NLGYVYQDVDIPWIKTRPTKRNKPKKKPKKQVASTVPF----GVGAALAEPISFTVEKAG 480
            LGYVYQDVDIPW+KT P+ R K  +K      +   F    G GAALA           
Sbjct: 429 ALGYVYQDVDIPWLKTAPSPRRKKLRKGGVANNNMPKFPSLLGTGAALAAESEL------ 488

Query: 481 MAQFPLTLDKIVRTEVKRPKKSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDR 540
             +FPL+L+ ++RT+VKRPKKSRS +EKEEEEEIL+I GIE D   + KFDV IN E+DR
Sbjct: 489 --EFPLSLESVIRTDVKRPKKSRSSKEKEEEEEILVISGIEFDPNTAVKFDVLINSEEDR 548

Query: 541 EIGPDNTEFAGSFVNLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFG 595
           EI  DNTEFAGSF ++PH+    N   LKT LRLG+ ELLEDL ++D +S+VVTLVP+  
Sbjct: 549 EIRADNTEFAGSFASVPHQ--NANMNNLKTCLRLGITELLEDLGIEDQDSVVVTLVPRIK 588

BLAST of Spg036083 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 7.0e-187
Identity = 341/607 (56.18%), Postives = 425/607 (70.02%), Query Frame = 0

Query: 1   MASLSPPPPATLSSAT-ATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQ-- 60
           M SLSPP   T +    AT    P  +NN   S   + ++   + A +V C A+N DQ  
Sbjct: 1   MTSLSPPVVTTPTVPNPATKPLSPFSQNNSQVSLLTKPKR---SFARKVSCKATNNDQND 60

Query: 61  ----KFDRRDVLLGLGGLYGASTLGPDSFAFASPVVTPPLDKCHLATLPDGTKDA-CCPP 120
               K DRR+VLLGLGGLYG + +G D FAFA P+  P + KC  A LP G     CCPP
Sbjct: 61  QAQSKLDRRNVLLGLGGLYGVAGMGTDPFAFAKPIAPPDVSKCGPADLPQGAVPTNCCPP 120

Query: 121 TAVNIKDFRPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCA 180
            +  I DF+   P+KLR+RP AH +D+ Y +KY +A++LMK LPD+DPR+F+QQA VHCA
Sbjct: 121 PSTKIIDFKLPAPAKLRIRPPAHAVDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHCA 180

Query: 181 YCHGGYDQVGFP-VQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKG 240
           YC G YDQVGFP +++Q+HNSWLFFPFHRYYLYF+EKILG+LI DPTF LPFWN+D+P G
Sbjct: 181 YCDGAYDQVGFPELELQIHNSWLFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAG 240

Query: 241 MRMPAIFADKNSPLYDQVRNLNHLPPALVDLNLDDAE--TDEETLVKRNLRVMYRQVVSG 300
           M +PAI+AD  SPLYD++R+ NH PP LVDL+ +  E    +ET +  NL++MYRQ+VS 
Sbjct: 241 MPLPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSN 300

Query: 301 ARTPSLFFGVPYRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGG-RDMGAFYSAARDPI 360
           ++   LFFG PYR GD  P+ GGGS+E  PH P+H+W GD+ Q    DMG FYSA RDPI
Sbjct: 301 SKNAKLFFGNPYRAGD-EPDPGGGSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDPI 360

Query: 361 FYAHHSNVDRLWSIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGY 420
           F+AHHSNVDR+WSIWKTLGGKR DL D D+L+S F+FYNE AE VRV VRDCL+ KNLGY
Sbjct: 361 FFAHHSNVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGY 420

Query: 421 VYQDVDIPWIKTRPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLTL 480
           VYQDVDIPW+ ++PT R        K   S V   +G A A   S +   AG  +FP++L
Sbjct: 421 VYQDVDIPWLSSKPTPRR------AKVALSKVAKKLGVAHAAVASSSKVVAG-TEFPISL 480

Query: 481 DKIVRTEVKRPK-KSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNT 540
              + T VKRPK K RSK+ KE+EEEIL+IEGIE D   + KFDVY+ND DD   GPD T
Sbjct: 481 GSKISTVVKRPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDDLPSGPDKT 540

Query: 541 EFAGSFVNLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFGFDQSPII 595
           EFAGSFV++PH  S K+   + T LRLGL +LLE++E +DD+S+VVTLVPKFG     + 
Sbjct: 541 EFAGSFVSVPH--SHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVVTLVPKFG----AVK 590

BLAST of Spg036083 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 1.9e-171
Identity = 318/587 (54.17%), Postives = 387/587 (65.93%), Query Frame = 0

Query: 30  YPSFGPRSRKFLINKAPRVLCSASNGDQK------------FDRRDVLLGLGGLYGASTL 89
           YP    + +   + K  R +  +SN +Q               RR+VL+GLGG+YG  TL
Sbjct: 27  YPFLQKQHQSSKLRKPKRQVTCSSNNNQNNPKEEQELSNIVGHRRNVLIGLGGIYG--TL 86

Query: 90  GPDSFAFASPVVTPPLDKC-HLATLPDGTKDA---CCPPTAVNIKDFRPTPPSKLRVRPA 149
             +  A ASP+  P L KC   + LP GT      CCPP +  I DF+      LRVR A
Sbjct: 87  ATNPSALASPISPPDLSKCVPPSDLPSGTTPPNINCCPPYSTKITDFKFPSNQPLRVRQA 146

Query: 150 AHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCHGGYDQVGFP-VQVQVHNS 209
           AHL+D +++EKYK+A +LMK LP NDPRNF QQAN+HCAYC G Y Q+GFP +++QVH S
Sbjct: 147 AHLVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAYCDGAYSQIGFPDLKLQVHGS 206

Query: 210 WLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRMPAIFADKNSPLYDQVRNL 269
           WLFFPFHR+YLYFYE+ILG LI DPTF LPFWNYDAP GM++P I+ADK SPLYD++RN 
Sbjct: 207 WLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLPTIYADKASPLYDELRNA 266

Query: 270 NHLPPALVDLNLDD--AETDEETLVKRNLRVMYRQVVSGARTPSLFFGVPYRGGDAAPEQ 329
           +H PP L+DLN  D  ++ D   L+K NL +MYRQV S  +T  LF G PYR GDA P Q
Sbjct: 267 SHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPYRAGDAEP-Q 326

Query: 330 GGGSVENVPHTPIHIWCGDSDQGG-RDMGAFYSAARDPIFYAHHSNVDRLWSIWKTLGGK 389
           G GS+ENVPH P+H W GD+ Q    DMG FYSAARDPIFY+HHSNVDRLW IWKTLGGK
Sbjct: 327 GAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWYIWKTLGGK 386

Query: 390 REDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGYVYQDVDIPWIKTRPTKRNKPK 449
           + D  D D+L S F+FY+E    VRV V+D LD+  LGY YQDV IPW K +P  R    
Sbjct: 387 KHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQDVPIPWEKAKPVPR---- 446

Query: 450 KKPKKQVASTVPFGVGAALAEPISFTVEKAG---MAQFPLTLDKIVRTEVKRPKKSRSKE 509
            + K Q    V    G     P  F V +        FPL L+  V   VKRPKK RSK+
Sbjct: 447 -RTKVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKVSAIVKRPKKLRSKK 506

Query: 510 EKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTEFAGSFVNLPHKVSGKNTT 569
           EKEEEEE+L+IEGIE     + KFDVYINDEDD+ +G  NTEFAGSFVN+PH   G    
Sbjct: 507 EKEEEEEVLVIEGIEFYMNIAIKFDVYINDEDDK-VGAGNTEFAGSFVNIPHSAHGHKNK 566

Query: 570 TLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFGFDQSPIIIGNIKI 594
            + TSLRLG+ +LLEDL V+ D++IVVTLVPK G  Q  + I N++I
Sbjct: 567 KIITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQ--VKINNVEI 602

BLAST of Spg036083 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.7e-161
Identity = 313/625 (50.08%), Postives = 407/625 (65.12%), Query Frame = 0

Query: 1   MASLSPPPPATLSSATATTYFV------PLF-RNNKYPSFGPRSRKFLINKAPRVLCSAS 60
           MASL  P   T S+A A T  +      PLF R +  P    RSR+F  +K   V C+++
Sbjct: 1   MASL--PWSLTTSTAIANTTNISAFPPSPLFQRASHVPVARNRSRRFAPSK---VSCNSA 60

Query: 61  NGDQ--------------KFDRRDVLLGLGGLYGAS-TLGPDS-FAFASPVVTPPLDKCH 120
           NGD               K DRR+VLLG+GGLYGA+  LG     AF +P+  P + KC 
Sbjct: 61  NGDPNSDSTSDVRETSSGKLDRRNVLLGIGGLYGAAGGLGATKPLAFGAPIQAPDISKCG 120

Query: 121 LATLPDG-TKDACCPPTAVNIKDFR-PTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCL 180
            AT+PDG T   CCPP    I DF+ P+  S +R RPAAHL+ ++Y+ KYK+A++L K L
Sbjct: 121 TATVPDGVTPTNCCPPVTTKIIDFQLPSSGSPMRTRPAAHLVSKEYLAKYKKAIELQKAL 180

Query: 181 PDNDPRNFRQQANVHCAYCHGGYDQVGF-PVQVQVHNSWLFFPFHRYYLYFYEKILGELI 240
           PD+DPR+F+QQANVHC YC G YDQVG+  +++QVH SWLF PFHRYYLYF E+IL +LI
Sbjct: 181 PDDDPRSFKQQANVHCTYCQGAYDQVGYTDLELQVHASWLFLPFHRYYLYFNERILAKLI 240

Query: 241 GDPTFGLPFWNYDAPKGMRMPAIFADKNSPLYDQVRNLNHLPPALVDLNLDDAE--TDEE 300
            DPTF LP+W +D P GM MP I+A   S LYD+ RN  HLPP ++DL+ D  E    ++
Sbjct: 241 DDPTFALPYWAWDNPDGMYMPTIYASSPSSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDD 300

Query: 301 TLVKRNLRVMYRQVVSGARTPSLFFGVPYRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQ 360
            L   NL +MY+Q+VSGA TP LF G PYR GDA  + G G++E+ PH  +H W G +D+
Sbjct: 301 ELKTDNLAIMYKQIVSGATTPKLFLGYPYRAGDAI-DPGAGTLEHAPHNIVHKWTGLADK 360

Query: 361 GGRDMGAFYSAARDPIFYAHHSNVDRLWSIWKTLGGK-REDLKDPDYLNSSFIFYNEKAE 420
              DMG FY+A RDPIF+ HH+NVDR+W+IWKT+GGK R+D  D D+L+++F+FY+E  +
Sbjct: 361 PSEDMGNFYTAGRDPIFFGHHANVDRMWNIWKTIGGKNRKDFTDTDWLDATFVFYDENKQ 420

Query: 421 AVRVYVRDCLDLKNLGYVYQDVDIPWIKTRPTKRNKPKKKPKKQVASTVPFGVGAALAEP 480
            V+V V DC+D   L Y YQD+ IPW+      + K   K  K        GV  A AE 
Sbjct: 421 LVKVKVSDCVDTSKLRYQYQDIPIPWLPKNTKAKAKTTTKSSKS-------GVAKA-AEL 480

Query: 481 ISFTVEKAGMAQFPLTLDKIVRTEVKRPKKSRSKEEKEEEEEILLIEGIEVDAGKSSKFD 540
              T+   G   FP  L+ ++R EV RPKKSRSK+EKE+EEE+LLI+GIE+D     KFD
Sbjct: 481 PKTTISSIG--DFPKALNSVIRVEVPRPKKSRSKKEKEDEEEVLLIKGIELDRENFVKFD 540

Query: 541 VYINDEDDREIGPDNTEFAGSFVNLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESI 597
           VYINDED     P N+EFAGSFVN+PHK   +  T  KT+LR  +NELLEDL  +DDES+
Sbjct: 541 VYINDEDYSVSRPKNSEFAGSFVNVPHKHMKEMKT--KTNLRFAINELLEDLGAEDDESV 600

BLAST of Spg036083 vs. ExPASy Swiss-Prot
Match: Q9MB14 (Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=2)

HSP 1 Score: 563.5 bits (1451), Expect = 2.8e-159
Identity = 301/606 (49.67%), Postives = 400/606 (66.01%), Query Frame = 0

Query: 3   SLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQ--KFD 62
           S +P  P +LSS+   +  +P   +  + +   R+  F      +V C+A NGD   K D
Sbjct: 11  SAAPKTPKSLSSSATISSPLPK-PSQIHIATAKRTHHF------KVSCNAPNGDSQPKLD 70

Query: 63  RRDVLLGLGGLYGASTLGPDSFAFASPVVTPPLDKCHLATL---PDGTKDACCPPTAVNI 122
           RRDVLLGLGGL GA++L  +  AFA P+  P + KC +      PD   D CCPP A N+
Sbjct: 71  RRDVLLGLGGLAGAASLINNPLAFAEPIHAPEISKCVVPPKDLPPDAIVDNCCPPLATNV 130

Query: 123 KDFR--PTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCH 182
             ++   T PS +++RPA H +D++YI K+++A++LMK LP +DPRNF QQA VHCAYC+
Sbjct: 131 IPYKVPKTSPSAMKIRPAIHRMDKEYIAKFEKAIRLMKELPADDPRNFYQQALVHCAYCN 190

Query: 183 GGYDQVGFP-VQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRM 242
           GGY Q  +P  ++QVHNSWLFFPFHR+YLYFYE+ILG+LIGDPTFGLPFWN+D P GM +
Sbjct: 191 GGYVQTDYPDKEIQVHNSWLFFPFHRWYLYFYERILGKLIGDPTFGLPFWNWDTPAGMLI 250

Query: 243 PAIFADKNSPLYDQVRNLNHLPPALVDLNLDDAETD--EETLVKRNLRVMYRQVVSGART 302
           P  F ++NSPLYD+ RN +HL P ++DL     +TD  ++  +  NL +MY+ +V+ A T
Sbjct: 251 PQYFRNQNSPLYDENRNQSHL-PLVMDLGYAGTDTDVTDQERISNNLALMYKSMVTNAGT 310

Query: 303 PSLFFGVPYRGGDAAPEQGGGSVENVPHTPIHIWCGD---SDQGGRDMGAFYSAARDPIF 362
             LF G PY+ GD    +GGGS+EN+PHTP+H W GD     Q G DMG FYSA RD +F
Sbjct: 311 AELFLGKPYKAGDDPVNKGGGSIENIPHTPVHRWVGDVKPRTQNGEDMGNFYSAGRDILF 370

Query: 363 YAHHSNVDRLWSIWKTLGGK--REDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLG 422
           Y HHSNVDR+W+IW+ LGGK  R D  D D+L+++FIFY+E  +AVRV V D LD + LG
Sbjct: 371 YCHHSNVDRMWTIWQQLGGKGRRRDFTDSDWLDATFIFYDENKQAVRVRVGDALDNQKLG 430

Query: 423 YVYQDVDIPWIKTRPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLT 482
           Y Y+  ++PW+ ++P      KKK      S  PF                     FPLT
Sbjct: 431 YKYEFTNLPWLNSKPL---PTKKKTGLAARSKAPFVTDV-----------------FPLT 490

Query: 483 LDKIVRTEVKRPKKSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNT 542
           LDK+V+ +V RPKKSRSKEEKE EEEIL I+GIEV   + +KFDVY+NDED+ E G +  
Sbjct: 491 LDKVVQVKVPRPKKSRSKEEKEAEEEILEIQGIEVAIDQYAKFDVYLNDEDEPEAGKEKA 550

Query: 543 EFAGSFVNLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFGFDQSPII 594
           E+AGSF +LPHK +G  +  ++TSL LGLNE LEDL  +DD++++VTL PK G     + 
Sbjct: 551 EYAGSFAHLPHKHTG--SKKIRTSLSLGLNEPLEDLGAEDDDAVLVTLAPKVG--GGVVT 584

BLAST of Spg036083 vs. ExPASy Swiss-Prot
Match: O81103 (Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PPO PE=1 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.7e-154
Identity = 294/614 (47.88%), Postives = 391/614 (63.68%), Query Frame = 0

Query: 5   SPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQ------- 64
           SP    T SS  +T  F     N    S   +S+ ++  ++  + C A+N +        
Sbjct: 6   SPTTMGTYSSLISTNSFSTFLPNKSQLSLSGKSKHYVARRS-SISCKATNNNNSNNQNEQ 65

Query: 65  ---------KFDRRDVLLGLGGLYGASTLGPDSFAFASPVVTPPLDKCHLATLPDGTKDA 124
                    K DRR++L+GLGGLYGA+TL    FAFA P+  P L  C  A +  G  + 
Sbjct: 66  QEESSRLLGKLDRRNILIGLGGLYGATTLDRKPFAFADPIAPPDLTTCKPAEITPGGSET 125

Query: 125 --CCPPTAVNIKDFRPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQ 184
             CCPP    IK F+P     LR  PAAH + ++Y+ K+K+A   M+ LPD+DPR+  QQ
Sbjct: 126 VPCCPPVTTKIKTFKPDLSIPLRTSPAAHQVTDEYLAKFKKAQAAMRALPDDDPRSMVQQ 185

Query: 185 ANVHCAYCHGGYDQVGF-PVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWN 244
           A VHCAYC+G Y QVGF    +QVH SWLFFPFHR YLYFYE+ILG+LI DPTF LP+WN
Sbjct: 186 AKVHCAYCNGAYPQVGFTDNDIQVHFSWLFFPFHRMYLYFYERILGKLIDDPTFALPYWN 245

Query: 245 YDAPKGMRMPAIFADKNSPLYDQVRNLNHLPPALVDLNL--DDAETDEETLVKRNLRVMY 304
           +D+P G  +P I+ D +SPLYDQ RN +H PP LVDL+    D + DE+T +  NL +MY
Sbjct: 246 WDSPVGFPIPDIYTDTSSPLYDQYRNADHQPPVLVDLSYGGKDDDVDEQTRIDENLAIMY 305

Query: 305 RQVVSGARTPSLFFGVPYRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGGR-DMGAFYS 364
           RQ+VSGA+TP LFFG  YR G+    +  G++EN+PH  IHIW GD  Q  + DMG FYS
Sbjct: 306 RQMVSGAKTPDLFFGHAYRAGNLNTGKYPGTIENMPHNNIHIWVGDPSQTHQEDMGNFYS 365

Query: 365 AARDPIFYAHHSNVDRLWSIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLD 424
           A RDP+FYAHH+NVDR+W+IWKTLGGKR+D+ D D+L++ F+FY+E AE VRV VRD L+
Sbjct: 366 AGRDPLFYAHHANVDRMWNIWKTLGGKRKDITDTDWLDAEFLFYDENAELVRVKVRDSLE 425

Query: 425 -LKNLGYVYQDVDIPWIKTRPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGM 484
             K L Y Y+ V +PW+ T+PT R K K K K +VA+T                      
Sbjct: 426 PEKQLRYNYEPVSLPWLFTKPTAR-KTKNKTKAKVAAT-------------------QLT 485

Query: 485 AQFPLTLDKIVRTEVKRPK-KSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDR 544
           ++FP TL ++   EV RPK + RSK+EK +EEE+L+I+ IE +  ++ KFDV+IND+ + 
Sbjct: 486 SKFPATLVEVTTVEVARPKPRKRSKKEKVDEEELLIIKDIEFEGTEAVKFDVFINDDAES 545

Query: 545 EIGPDNTEFAGSFVNLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFG 595
               D +EFAGSFV++P   + K  T  KT+L+LG+ +LLEDL  +DD S++VTLVP+  
Sbjct: 546 LSRRDKSEFAGSFVHVPQGKTTKAKT--KTNLKLGITDLLEDLGAEDDSSVLVTLVPR-- 594

BLAST of Spg036083 vs. ExPASy TrEMBL
Match: A0A0U2D7M5 (Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 573/598 (95.82%), Postives = 576/598 (96.32%), Query Frame = 0

Query: 1   MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQKFD 60
           MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQKFD
Sbjct: 1   MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQKFD 60

Query: 61  RRDVLLGLGGLYGASTLGPDSFAFASPVVTPPLDKCHLATLPDGTKDACCPPTAVNIKDF 120
           RRDVLLGLGGLYGASTLGPDSFAFASPVVTPPLDKCHLATLPDGTKDACCPPTAVNIKDF
Sbjct: 61  RRDVLLGLGGLYGASTLGPDSFAFASPVVTPPLDKCHLATLPDGTKDACCPPTAVNIKDF 120

Query: 121 RPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCHGGYDQ 180
           RPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCHGGYDQ
Sbjct: 121 RPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCHGGYDQ 180

Query: 181 VGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRMPAIFAD 240
           VGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRMPAIFAD
Sbjct: 181 VGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRMPAIFAD 240

Query: 241 KNSPLYDQVRNLNHLPPALVDLNLDDAETDEETLVKRNLRVMYRQVVSGARTPSLFFGVP 300
           KNSPLYDQVRNLNHLPPALVDLNLDDAETDEETLVKRNLRVMYRQVVSGARTPSLFFGVP
Sbjct: 241 KNSPLYDQVRNLNHLPPALVDLNLDDAETDEETLVKRNLRVMYRQVVSGARTPSLFFGVP 300

Query: 301 YRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGGRDMGAFYSAARDPIFYAHHSNVDRLW 360
           YRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGGRDMGAFYSAARDPIFYAHHSNVDRLW
Sbjct: 301 YRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGGRDMGAFYSAARDPIFYAHHSNVDRLW 360

Query: 361 SIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGYVYQDVDIPWIKT 420
           SIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGYVYQD DIPWIKT
Sbjct: 361 SIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGYVYQDADIPWIKT 420

Query: 421 RPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLTLDKIVRTEVKRPK 480
           RPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLTLDKIVRTEVKRPK
Sbjct: 421 RPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLTLDKIVRTEVKRPK 480

Query: 481 KSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTEFAGSFVNLPHKV 540
           KSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTEFAGSFVNLPHKV
Sbjct: 481 KSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTEFAGSFVNLPHKV 540

Query: 541 SGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFGFDQSPIIIGNIKIELLIE 599
           SGKNTTTLKTSL  G    L+       ++      P  GFDQSPIIIGNIKIELLIE
Sbjct: 541 SGKNTTTLKTSLSWGSTNCLKIWRWMMMKASWSLWCPSLGFDQSPIIIGNIKIELLIE 598

BLAST of Spg036083 vs. ExPASy TrEMBL
Match: A0A1L5JHU3 (Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 8.6e-212
Identity = 378/600 (63.00%), Postives = 459/600 (76.50%), Query Frame = 0

Query: 4   LSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQK-FDRR 63
           ++  PPA LSSA  TT F P   NNK P    R  K+  +KAPR+LCSASNG Q   DRR
Sbjct: 1   MATSPPA-LSSAATTTTFGPFLPNNKNPPPNLRRTKYFSSKAPRILCSASNGGQNHIDRR 60

Query: 64  DVLLGLGGLYGASTLGPDSFAFASPVVTPPLDKCHLATLPDGTKDA-CCPPTAVNIKDFR 123
           D+L+GLGGLYGASTL   S AFA+PV TP + KC    LP G     CCPP    + DFR
Sbjct: 61  DILIGLGGLYGASTLSQGSLAFAAPVQTPNISKCGQPDLPPGADPINCCPPPTTAVVDFR 120

Query: 124 PTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCHGGYDQV 183
           P  P KLRVRPAAHL D+ Y+EKYKRAV+LMK LPD+DPR+F+QQANVHCAYC+G YDQV
Sbjct: 121 PQAPGKLRVRPAAHLADQNYVEKYKRAVELMKALPDDDPRSFKQQANVHCAYCNGAYDQV 180

Query: 184 GFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRMPAIFADK 243
           GFPV+VQVHNSWLFFPFHRYYL+F+EKILGELIGDP+F LPFWNYDAP GM+MPA++AD+
Sbjct: 181 GFPVEVQVHNSWLFFPFHRYYLHFHEKILGELIGDPSFALPFWNYDAPGGMKMPAMYADE 240

Query: 244 NSPLYDQVRNLNHLPPALVDLNLD--DAETDEETLVKRNLRVMYRQVVSGARTPSLFFGV 303
            S LYD++RN NHLPP LVDL+    D    +E  ++ NL +MYRQ+VS +RTPSLFFG 
Sbjct: 241 KSSLYDELRNRNHLPPTLVDLDFGGVDPTIGDEAQIRSNLSIMYRQMVSNSRTPSLFFGN 300

Query: 304 PYRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGG-RDMGAFYSAARDPIFYAHHSNVDR 363
            Y  GD  P  GGG VEN+PH P+HIWCGD+++    +MG FYSAARDPIFYAHHSN+DR
Sbjct: 301 AYLAGD-EPSPGGGLVENIPHGPVHIWCGDNNEPNFENMGNFYSAARDPIFYAHHSNIDR 360

Query: 364 LWSIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGYVYQDVDIPWI 423
           LWS+WKTLGG+R+DL++ D+LN+SFIFY+EKA+AVRV VRDCLD +NLGYVYQDV +PW+
Sbjct: 361 LWSVWKTLGGRRQDLQNADWLNASFIFYDEKAQAVRVRVRDCLDSRNLGYVYQDVQLPWL 420

Query: 424 KTRPTKRNKPKKKPKKQVASTVPFGVGAALAE----PISFTVEKAGMAQFPLTLDKIVRT 483
           ++RPT R     + +K VA  +PFGVG ALA     P S  +  A + +FP+TL+  +  
Sbjct: 421 ESRPTPRR--ALRAQKAVAH-LPFGVGPALAAGEPGPSSSRLRPAKI-KFPITLNSPISM 480

Query: 484 EVKRPKKSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTEFAGSFV 543
           EVKRPK+SRSK+EK+EEEEI++I GIE DA K+ KFDV+INDEDD+EI PDNTEFAGSFV
Sbjct: 481 EVKRPKRSRSKKEKDEEEEIIVIYGIEFDANKAIKFDVFINDEDDKEIRPDNTEFAGSFV 540

Query: 544 NLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFGFDQSPIIIGNIKIE 595
           N+PHK   K    +KT LRLG+ ELLEDL+ DDD+S+ VTLVP+ G  +  + I  IKIE
Sbjct: 541 NVPHKHGHKK--NVKTGLRLGITELLEDLDADDDDSVRVTLVPRSG--KGLVSIAGIKIE 590

BLAST of Spg036083 vs. ExPASy TrEMBL
Match: A0A6J1KRC0 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495765 PE=3 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 4.4e-200
Identity = 371/601 (61.73%), Postives = 447/601 (74.38%), Query Frame = 0

Query: 1   MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASNGDQKFD 60
           MASL  PPPA LS+AT        FR    PS  P + K+L N A RVLCSA+NG  K D
Sbjct: 1   MASL--PPPA-LSTAT--------FR----PSSRPPTSKYLPNNAARVLCSAANG--KID 60

Query: 61  RRDVLLGL--GGLYGASTLGPDS-FAFASPVVTPPLDKCHLATLPDGTKDA-CCPPTAVN 120
           RRDVL+G+  GG+YGAS    DS FA A+PV TP + KC    LP G+    CCPP + +
Sbjct: 61  RRDVLIGIGAGGIYGASAFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPS 120

Query: 121 IKDFRPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCAYCHG 180
           I DFRP  P KLRVRPAAHL D+ YI+KYKRAV+LMK LP +DPR+F QQANVHCAYC+G
Sbjct: 121 IVDFRPPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNG 180

Query: 181 GYDQVGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGMRMPA 240
           GYDQVGFPV+VQVHNSWLFFPFHRYYLYFYEKILGELIGDPTF LPFWNYDAP GMRMPA
Sbjct: 181 GYDQVGFPVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPA 240

Query: 241 IFADKNSPLYDQVRNLNHLPPALVDLNLD--DAETDEETLVKRNLRVMYRQVVSGARTPS 300
           ++AD NS LYDQ+RN NHLPP L+DL+ D  D+   +   ++ NLR+MYRQ+VS +RTP 
Sbjct: 241 MYADHNSSLYDQLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPK 300

Query: 301 LFFGVPYRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGG-RDMGAFYSAARDPIFYAHH 360
           LFFG  Y  GD  P  GGGSVEN+PH  +H WCGD+++    +MG FYSAARDPIFY+HH
Sbjct: 301 LFFGSAYVAGD-EPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHH 360

Query: 361 SNVDRLWSIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGYVYQDV 420
           SNVDR+WS+WKTLGG+R DL+ P++L++SFIFYNEKA+AVRV VRDCLD KNLGY+YQDV
Sbjct: 361 SNVDRMWSVWKTLGGRRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDV 420

Query: 421 DIPWIKTRPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLTLDKIVR 480
           ++PW++TRPT R     + +K V S VP           S  V+   M + P+TL   + 
Sbjct: 421 ELPWLETRPTPRR--ALRAQKSVDSNVP-----------SRRVKPPKM-KLPVTLKSAIS 480

Query: 481 TEVKRPKKSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTEFAGSF 540
           TEVKR KKSR ++EK+EEEEIL+I+GIE D   S K D+YINDEDDREI  DNTEFAGSF
Sbjct: 481 TEVKRGKKSRGRKEKDEEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAGSF 540

Query: 541 VNLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFGFDQSPIIIGNIKI 595
           VN+PHK   K    +KT LRLG++E+LEDL+ DDD+SI+VT++P+ G     + I  IKI
Sbjct: 541 VNVPHKHGHKK--KVKTGLRLGISEVLEDLDADDDDSIIVTIIPRLG--AGLVSIAEIKI 565

BLAST of Spg036083 vs. ExPASy TrEMBL
Match: A0A6J1CGK8 (polyphenol oxidase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111010634 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 2.9e-199
Identity = 370/614 (60.26%), Postives = 445/614 (72.48%), Query Frame = 0

Query: 1   MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAP---RVLCSASNGDQ 60
           MAS+ PP P +L         +P FR           RKF++NK     RV+CSASN   
Sbjct: 9   MASVCPPLPPSL---------LPSFR-----------RKFVVNKNNKHCRVVCSASNKHD 68

Query: 61  K--------FDRRDVLLGLGGLYGASTLGP--DSFAFASPVVTPPLDKCHLATLPDGTKD 120
                     DRRDVL+GLGGLYGAS+  P  DSFA A+PV TP + KC  A + D  K 
Sbjct: 69  SQSQSPGNMVDRRDVLIGLGGLYGASSFAPEXDSFAIAAPVDTPQITKCGPAQVRD-KKV 128

Query: 121 ACCPPTAVNIKDFRPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQA 180
            CCPP ++ IKDFRPTPP KLRVRPAAHL+D+ Y++KY+RAV+LM+ LPD+DPR+F QQA
Sbjct: 129 ECCPPPSIKIKDFRPTPPRKLRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQA 188

Query: 181 NVHCAYCHGGYDQVGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYD 240
           N+HCAYC+GGY Q+GFPV++QVHNSWLFFPFHR+YLYF+EKILGELIGDPTF LPFWNYD
Sbjct: 189 NIHCAYCNGGYKQLGFPVELQVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYD 248

Query: 241 APKGMRMPAIFADKNSPLYDQVRNLNHLPPALVDL--NLDDAETDEETLVKRNLRVMYRQ 300
           AP GM  PAIFA++NSPLYD+ RN NH+PP +VDL  N  D   D+E  V+ NLRVMYRQ
Sbjct: 249 APAGMTFPAIFANQNSPLYDEFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQ 308

Query: 301 VVSGARTPSLFFGVPYRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQ-GGRDMGAFYSAA 360
           +VSG+RT SLFFG PYR GD  P  G GSVENVPH PIH WCG+  Q  G DMG FYSA 
Sbjct: 309 MVSGSRTRSLFFGRPYRAGD-DPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAG 368

Query: 361 RDPIFYAHHSNVDRLWSIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLK 420
           RDP+FY+HH+NVDR+W IWKTLG +R D+++PD+LN+SF+FYNEK EAVRV VRDCLD  
Sbjct: 369 RDPVFYSHHANVDRMWCIWKTLGRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTT 428

Query: 421 NLGYVYQDVDIPWIKTRPTKRNKPKKKPKKQVASTVPF----GVGAALAEPISFTVEKAG 480
            LGYVYQDVDIPW+KT P+ R K  +K      +   F    G GAALA           
Sbjct: 429 ALGYVYQDVDIPWLKTAPSPRRKKLRKGGVANNNMPKFPSLLGTGAALAAESEL------ 488

Query: 481 MAQFPLTLDKIVRTEVKRPKKSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDR 540
             +FPL+L+ ++RT+VKRPKKSRS +EKEEEEEIL+I GIE D   + KFDV IN E+DR
Sbjct: 489 --EFPLSLESVIRTDVKRPKKSRSSKEKEEEEEILVISGIEFDPNTAVKFDVLINSEEDR 548

Query: 541 EIGPDNTEFAGSFVNLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFG 595
           EI  DNTEFAGSF ++PH+    N   LKT LRLG+ ELLEDL ++D +S+VVTLVP+  
Sbjct: 549 EIRADNTEFAGSFASVPHQ--NANMNNLKTCLRLGITELLEDLGIEDQDSVVVTLVPRIK 588

BLAST of Spg036083 vs. ExPASy TrEMBL
Match: A0A5D3DS75 (Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00570 PE=3 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.9e-198
Identity = 355/607 (58.48%), Postives = 441/607 (72.65%), Query Frame = 0

Query: 1   MASLSPPPPATLSSATATTYFVPLFRNNKYPSFGPRSRKFLINKAPRVLCSASN------ 60
           MA LS PP  TL+         P     K            I+   R+ C ASN      
Sbjct: 1   MAFLSHPPSLTLT---------PFLHKTK-----------TISITSRISCKASNHNDQNT 60

Query: 61  GDQKFDRRDVLLGLGGLYGASTLGPD-SFAFASPVVTPPLDKCHLATLPDGTKDA-CCPP 120
            + K DRRD+L+GL GLYG+STL     FA A+P+ TP + KC    LP G     CCPP
Sbjct: 61  SNPKIDRRDILIGLTGLYGSSTLSQSPHFALAAPIQTPNISKCGPPDLPPGADPTNCCPP 120

Query: 121 TAVNIKDFRPTPPSKLRVRPAAHLIDEKYIEKYKRAVQLMKCLPDNDPRNFRQQANVHCA 180
            A  I DF+P PP+KLRVRPAAHL D+ YIEKYK+A++LMK LP++DPR+F QQANVHCA
Sbjct: 121 PATTILDFQPPPPTKLRVRPAAHLADQNYIEKYKKAIELMKALPEDDPRSFTQQANVHCA 180

Query: 181 YCHGGYDQVGFPVQVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFGLPFWNYDAPKGM 240
           YC+G YDQ+GFPV+VQVHNSWLFFPFHR+YLYF+EKILGELIGD +F LPFWNYDAP GM
Sbjct: 181 YCNGAYDQIGFPVEVQVHNSWLFFPFHRFYLYFHEKILGELIGDSSFALPFWNYDAPGGM 240

Query: 241 RMPAIFADKNSPLYDQVRNLNHLPPALVDLN---LDDAETDEETLVKRNLRVMYRQVVSG 300
           +MP ++A++ S LYD++RN NHLPP LVDL+   +DD    ++  ++ NL +MYRQ+VSG
Sbjct: 241 KMPRMYAEEKSSLYDELRNENHLPPRLVDLDFGGVDDPNIGDDAQIRSNLSIMYRQMVSG 300

Query: 301 ARTPSLFFGVPYRGGDAAPEQGGGSVENVPHTPIHIWCGDSDQGG-RDMGAFYSAARDPI 360
           +RTPSLFFG  Y  GD  P  GGGSVEN+PH P+H+WCGD+DQ    +MG FYSAARDPI
Sbjct: 301 SRTPSLFFGSAYVEGD-EPSPGGGSVENIPHGPVHVWCGDNDQPNFENMGNFYSAARDPI 360

Query: 361 FYAHHSNVDRLWSIWKTLGGKREDLKDPDYLNSSFIFYNEKAEAVRVYVRDCLDLKNLGY 420
           F+AHHSNVDRLWS+WKTLGG+R+DL++P++LN+SFIFYNEKA+ VRV+VRDCLD K+LGY
Sbjct: 361 FFAHHSNVDRLWSVWKTLGGRRKDLQNPEWLNASFIFYNEKAQPVRVHVRDCLDSKSLGY 420

Query: 421 VYQDVDIPWIKTRPTKRNKPKKKPKKQVASTVPFGVGAALAEPISFTVEKAGMAQFPLTL 480
            YQ VD+PW++TRPT        P++   +TV FG+ +ALA   S +  K    +FP+T+
Sbjct: 421 TYQPVDLPWLQTRPT--------PRRAQKTTVAFGIASALAAGPSSSRVKPANIKFPITV 480

Query: 481 DKIVRTEVKRPKKSRSKEEKEEEEEILLIEGIEVDAGKSSKFDVYINDEDDREIGPDNTE 540
              V  EVKRPKKSRSK+EKEEEEE+L+I GI  D     KFDV+INDEDD+EI PDNTE
Sbjct: 481 KSPVSVEVKRPKKSRSKKEKEEEEEVLVIYGIGFDPNNGIKFDVFINDEDDKEIKPDNTE 540

Query: 541 FAGSFVNLPHKVSGKNTTTLKTSLRLGLNELLEDLEVDDDESIVVTLVPKFGFDQSPIII 596
           FAGSFVN+PHK + K    +KT LR+G+ E+LEDLE D+D+SI+VT+VPK G       I
Sbjct: 541 FAGSFVNVPHKQAHKK--KVKTGLRVGITEVLEDLEADNDDSIIVTIVPKIG--AGLFSI 574

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AKN08990.10.0e+0095.82polyphenol oxidase [Luffa aegyptiaca][more]
APO14262.11.8e-21163.00polyphenol oxidase 3 [Luffa aegyptiaca][more]
XP_038903169.17.0e-20060.64polyphenol oxidase, chloroplastic-like [Benincasa hispida][more]
XP_023001713.19.1e-20061.73polyphenol oxidase, chloroplastic-like [Cucurbita maxima][more]
XP_022139808.15.9e-19960.26polyphenol oxidase, chloroplastic-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
P433097.0e-18756.18Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1[more]
Q062151.9e-17154.17Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1[more]
P433111.7e-16150.08Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
Q9MB142.8e-15949.67Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=... [more]
O811032.7e-15447.88Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PP... [more]
Match NameE-valueIdentityDescription
A0A0U2D7M50.0e+0095.82Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A1L5JHU38.6e-21263.00Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A6J1KRC04.4e-20061.73polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114957... [more]
A0A6J1CGK82.9e-19960.26polyphenol oxidase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A5D3DS751.9e-19858.48Polyphenol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G0... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002227Tyrosinase copper-binding domainPRINTSPR00092TYROSINASEcoord: 345..363
score: 58.32
coord: 190..207
score: 58.96
coord: 319..330
score: 38.1
coord: 221..226
score: 55.1
IPR002227Tyrosinase copper-binding domainPFAMPF00264Tyrosinasecoord: 160..365
e-value: 7.0E-34
score: 118.0
IPR002227Tyrosinase copper-binding domainPROSITEPS00498TYROSINASE_2coord: 346..357
IPR002227Tyrosinase copper-binding domainPROSITEPS00497TYROSINASE_1coord: 190..207
IPR016213Polyphenol oxidasePIRSFPIRSF000290PPO_plantcoord: 1..597
e-value: 1.2E-238
score: 791.7
IPR008922Uncharacterised domain, di-copper centreGENE3D1.10.1280.10coord: 86..419
e-value: 2.4E-110
score: 371.1
IPR008922Uncharacterised domain, di-copper centreSUPERFAMILY48056Di-copper centre-containing domaincoord: 102..417
IPR022739Polyphenol oxidase, central domainPFAMPF12142PPO1_DWLcoord: 372..423
e-value: 1.2E-22
score: 79.4
IPR022740Polyphenol oxidase, C-terminalPFAMPF12143PPO1_KFDVcoord: 464..595
e-value: 9.1E-49
score: 164.9
NoneNo IPR availablePIRSRPIRSR000290-1PIRSR000290-1coord: 87..595
e-value: 1.6E-213
score: 707.9
NoneNo IPR availablePANTHERPTHR11474:SF94POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKEcoord: 44..595
NoneNo IPR availablePANTHERPTHR11474TYROSINASE FAMILY MEMBERcoord: 44..595

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg036083.1Spg036083.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046148 pigment biosynthetic process
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity