Spg036053 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg036053 vs. NCBI nr
Match: XP_023002177.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002870.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002871.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 463.0 bits (1190), Expect = 1.8e-126
Identity = 218/279 (78.14%), Postives = 245/279 (87.81%), Query Frame = 0

Query: 1   MMLFGPSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRL 60
           M+LF PSLS AT+P NIFILAGQSNMAGRGGVEN++ G KL WDG VP  CQ DPSILRL
Sbjct: 12  MILFHPSLSWATSPTNIFILAGQSNMAGRGGVENNQKG-KLEWDGKVPLECQSDPSILRL 71

Query: 61  NPQCQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIE 120
           NP  QWEIA+EP+H GID+  KTPGIGP +  A Q + K GQKAG+VGLVPCA+GGTLIE
Sbjct: 72  NPARQWEIAQEPLHLGIDI-GKTPGIGPGIPFAHQFKAKAGQKAGIVGLVPCARGGTLIE 131

Query: 121 QWMKNPSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFF 180
           QW+KNPSNP+ TFYQNFIERIK S+KEGGVVRALFW+ GESDAAMSDTAHRYKDNLKKF 
Sbjct: 132 QWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTAHRYKDNLKKFI 191

Query: 181 TDVRNDIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPI 240
           TD+RNDIKPRFLP+IIVK ++YDFFMKHDT+DLP VRAA++A+Q ELPD++TIDSW+LPI
Sbjct: 192 TDIRNDIKPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPDIITIDSWELPI 251

Query: 241 NLTTHEGFNLDHGHFNTTTEIALGKWLADTYLSHYGHLL 280
           N TT EGF LDHGHFNT TEIALGKWLADTYL+HY HLL
Sbjct: 252 NFTTFEGFCLDHGHFNTATEIALGKWLADTYLAHYSHLL 288

BLAST of Spg036053 vs. NCBI nr
Match: XP_038886442.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 459.5 bits (1181), Expect = 2.0e-125
Identity = 217/278 (78.06%), Postives = 243/278 (87.41%), Query Frame = 0

Query: 2   MLFGPSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRLN 61
           MLFG SLSRA +PNNIFILAGQSNMAGRGGVEN++  ++L WDG +PP CQ DPSILRLN
Sbjct: 13  MLFGSSLSRAASPNNIFILAGQSNMAGRGGVENNQV-RELEWDGLIPPECQSDPSILRLN 72

Query: 62  PQCQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIEQ 121
           P  QWEIAREP+H GID+ +KT GIGP M  A Q+  KVG +AG VGLVPCA+GGT+IEQ
Sbjct: 73  PALQWEIAREPLHEGIDI-NKTVGIGPGMPFAHQLLTKVGPRAGTVGLVPCARGGTIIEQ 132

Query: 122 WMKNPSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFFT 181
           W+KNPSNP+ TFY+NFIERIKASDKEGGVVRALFW  GESDAAMSDTA+RYKDNLK FFT
Sbjct: 133 WIKNPSNPDATFYKNFIERIKASDKEGGVVRALFWFQGESDAAMSDTANRYKDNLKNFFT 192

Query: 182 DVRNDIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPIN 241
           D+RNDIKPRFLPII+VK  LYDF MKHDT+DLP VRAAQ+A+  ELPD+VTID+ KLPIN
Sbjct: 193 DIRNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAQDAVSKELPDIVTIDALKLPIN 252

Query: 242 LTTHEGFNLDHGHFNTTTEIALGKWLADTYLSHYGHLL 280
           + THEGFN DHGHFNTTT+I LGKWLADTYLSHYGHLL
Sbjct: 253 VDTHEGFNQDHGHFNTTTQITLGKWLADTYLSHYGHLL 288

BLAST of Spg036053 vs. NCBI nr
Match: XP_023536885.1 (probable carbohydrate esterase At4g34215 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023536886.1 probable carbohydrate esterase At4g34215 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 458.0 bits (1177), Expect = 5.9e-125
Identity = 215/279 (77.06%), Postives = 244/279 (87.46%), Query Frame = 0

Query: 1   MMLFGPSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRL 60
           M+LF PSLS AT+P NIFILAGQSNMAGRGGVE  ++G KLVWDG VP  CQ DPSILRL
Sbjct: 12  MILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNG-KLVWDGKVPLECQSDPSILRL 71

Query: 61  NPQCQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIE 120
           NP+ QWEIA EP+H GID+   TPGIGP +  A Q +EK GQKAG VGLVPCA+GGTLIE
Sbjct: 72  NPERQWEIAHEPLHLGIDI-SHTPGIGPGIPFAHQFKEKAGQKAGTVGLVPCARGGTLIE 131

Query: 121 QWMKNPSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFF 180
           QW+KNPSNP+ TFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DTA RYKDNLKKF 
Sbjct: 132 QWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFI 191

Query: 181 TDVRNDIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPI 240
           TD+RNDIKPRFLP+IIVK ++YDFFMKHDT++LP VRAA++A+Q ELPD++TIDSW+LPI
Sbjct: 192 TDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPI 251

Query: 241 NLTTHEGFNLDHGHFNTTTEIALGKWLADTYLSHYGHLL 280
           NLTT EGF+ DHGHFNT TEI LGKWLADTYL+HYGHLL
Sbjct: 252 NLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288

BLAST of Spg036053 vs. NCBI nr
Match: KAG6585835.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 457.6 bits (1176), Expect = 7.7e-125
Identity = 215/279 (77.06%), Postives = 244/279 (87.46%), Query Frame = 0

Query: 1   MMLFGPSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRL 60
           M+LF PSLS AT+P NIFILAGQSNMAGRGGVE +++G +LVWDG VP  CQ DPSILRL
Sbjct: 1   MILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKNQNG-ELVWDGKVPLECQSDPSILRL 60

Query: 61  NPQCQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIE 120
           NP+CQWEIA EP+H GID+   TPGIG  +  A Q++EK GQKAG VGLVPCA+GGTLIE
Sbjct: 61  NPECQWEIAHEPLHLGIDI-GHTPGIGLGIPFAHQLKEKAGQKAGTVGLVPCARGGTLIE 120

Query: 121 QWMKNPSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFF 180
           QW+KNPSNP+ TFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DTA RYKDNLKKF 
Sbjct: 121 QWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFI 180

Query: 181 TDVRNDIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPI 240
           TD+RNDIKPRFLP+IIVK  LYDFFMKHDT++LP VR A++A+Q ELPDV+TIDSW+LPI
Sbjct: 181 TDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVREAEDAVQKELPDVITIDSWELPI 240

Query: 241 NLTTHEGFNLDHGHFNTTTEIALGKWLADTYLSHYGHLL 280
           NLTT EGF+ DHGH NT TEIALGKWLADTYL+HYGHLL
Sbjct: 241 NLTTFEGFSWDHGHLNTATEIALGKWLADTYLAHYGHLL 277

BLAST of Spg036053 vs. NCBI nr
Match: XP_022973316.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_022973317.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 455.7 bits (1171), Expect = 2.9e-124
Identity = 213/279 (76.34%), Postives = 243/279 (87.10%), Query Frame = 0

Query: 2   MLFGPSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRLN 61
           +L  PSLS AT+P NIFILAGQSNMAGRGGVE   +G +LVWDG VP  CQ DPSILR N
Sbjct: 13  ILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPTG-ELVWDGKVPSECQSDPSILRFN 72

Query: 62  PQCQWEIAREPIHAGIDL-PDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIE 121
           P+ QWEIA EP+H GID+   KTPGIGP +  A Q++EK GQKAG+VGLVPCA+GGTLIE
Sbjct: 73  PERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIE 132

Query: 122 QWMKNPSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFF 181
           QW+KNPSNP+ TFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DTA RYKDNLKKF 
Sbjct: 133 QWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFI 192

Query: 182 TDVRNDIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPI 241
           TD+RNDIKPRFLP+IIVK  LYDFFMKHDT++LP VRAA++A+Q ELPD++TIDSW+LP+
Sbjct: 193 TDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPM 252

Query: 242 NLTTHEGFNLDHGHFNTTTEIALGKWLADTYLSHYGHLL 280
           NLTT EGF+ DHGHFNT TEIALGKWLADTYL+HYGHLL
Sbjct: 253 NLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 290

BLAST of Spg036053 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 180.3 bits (456), Expect = 3.1e-44
Identity = 101/270 (37.41%), Postives = 146/270 (54.07%), Query Frame = 0

Query: 6   PSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRLNPQCQ 65
           P +     PN IFIL+GQSNMAGRGGV  D    + VWD  +PP C P+ SILRL+   +
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 66  WEIAREPIHAGIDLPDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIEQWMKN 125
           WE A EP+H  ID   K  G+GP MA A  ++ ++   + V+GLVPCA GGT I++W + 
Sbjct: 73  WEEAHEPLHVDID-TGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER- 132

Query: 126 PSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFFTDVRN 185
                   Y+  ++R + S K GG ++A+ W+ GESD      A  Y +N+ +   ++R+
Sbjct: 133 ----GSHLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRH 192

Query: 186 DIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPINLTTH 245
           D+    LPII V        +      +  VR AQ  L  +L +VV +D+  LP+     
Sbjct: 193 DLNLPSLPIIQVA-------IASGGGYIDKVREAQLGL--KLSNVVCVDAKGLPL----- 252

Query: 246 EGFNLDHGHFNTTTEIALGKWLADTYLSHY 276
                D+ H  T  ++ LG  LA  YLS++
Sbjct: 253 ---KSDNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of Spg036053 vs. ExPASy TrEMBL
Match: A0A6J1KIR8 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496116 PE=4 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 8.8e-127
Identity = 218/279 (78.14%), Postives = 245/279 (87.81%), Query Frame = 0

Query: 1   MMLFGPSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRL 60
           M+LF PSLS AT+P NIFILAGQSNMAGRGGVEN++ G KL WDG VP  CQ DPSILRL
Sbjct: 12  MILFHPSLSWATSPTNIFILAGQSNMAGRGGVENNQKG-KLEWDGKVPLECQSDPSILRL 71

Query: 61  NPQCQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIE 120
           NP  QWEIA+EP+H GID+  KTPGIGP +  A Q + K GQKAG+VGLVPCA+GGTLIE
Sbjct: 72  NPARQWEIAQEPLHLGIDI-GKTPGIGPGIPFAHQFKAKAGQKAGIVGLVPCARGGTLIE 131

Query: 121 QWMKNPSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFF 180
           QW+KNPSNP+ TFYQNFIERIK S+KEGGVVRALFW+ GESDAAMSDTAHRYKDNLKKF 
Sbjct: 132 QWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTAHRYKDNLKKFI 191

Query: 181 TDVRNDIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPI 240
           TD+RNDIKPRFLP+IIVK ++YDFFMKHDT+DLP VRAA++A+Q ELPD++TIDSW+LPI
Sbjct: 192 TDIRNDIKPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPDIITIDSWELPI 251

Query: 241 NLTTHEGFNLDHGHFNTTTEIALGKWLADTYLSHYGHLL 280
           N TT EGF LDHGHFNT TEIALGKWLADTYL+HY HLL
Sbjct: 252 NFTTFEGFCLDHGHFNTATEIALGKWLADTYLAHYSHLL 288

BLAST of Spg036053 vs. ExPASy TrEMBL
Match: A0A6J1I774 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111471873 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.4e-124
Identity = 213/279 (76.34%), Postives = 243/279 (87.10%), Query Frame = 0

Query: 2   MLFGPSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRLN 61
           +L  PSLS AT+P NIFILAGQSNMAGRGGVE   +G +LVWDG VP  CQ DPSILR N
Sbjct: 13  ILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPTG-ELVWDGKVPSECQSDPSILRFN 72

Query: 62  PQCQWEIAREPIHAGIDL-PDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIE 121
           P+ QWEIA EP+H GID+   KTPGIGP +  A Q++EK GQKAG+VGLVPCA+GGTLIE
Sbjct: 73  PERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIE 132

Query: 122 QWMKNPSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFF 181
           QW+KNPSNP+ TFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DTA RYKDNLKKF 
Sbjct: 133 QWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFI 192

Query: 182 TDVRNDIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPI 241
           TD+RNDIKPRFLP+IIVK  LYDFFMKHDT++LP VRAA++A+Q ELPD++TIDSW+LP+
Sbjct: 193 TDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPM 252

Query: 242 NLTTHEGFNLDHGHFNTTTEIALGKWLADTYLSHYGHLL 280
           NLTT EGF+ DHGHFNT TEIALGKWLADTYL+HYGHLL
Sbjct: 253 NLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 290

BLAST of Spg036053 vs. ExPASy TrEMBL
Match: A0A6J1GJF6 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454406 PE=4 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 1.0e-122
Identity = 212/279 (75.99%), Postives = 243/279 (87.10%), Query Frame = 0

Query: 1   MMLFGPSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRL 60
           M+LF PSLS AT+P NIFILAGQSNMAGRGGVE  ++G KLVWDG VP  CQ DPSILRL
Sbjct: 12  MILFNPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNG-KLVWDGKVPLECQFDPSILRL 71

Query: 61  NPQCQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIE 120
           NP+ QWEIA EP+H GID+   TPGIGP +  A Q +EK GQKAG+VGLVPCA+GGTLIE
Sbjct: 72  NPERQWEIAHEPLHLGIDI-SHTPGIGPGIPFAHQFKEKAGQKAGIVGLVPCARGGTLIE 131

Query: 121 QWMKNPSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFF 180
           QW+KNPSNP+ TFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DTA RYKDNLKKF 
Sbjct: 132 QWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFI 191

Query: 181 TDVRNDIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPI 240
           TD+RNDIKPRFLP+IIVK +LYDFFMKHDT++LP VRAA++A+Q ELPD++TIDS +LP+
Sbjct: 192 TDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPV 251

Query: 241 NLTTHEGFNLDHGHFNTTTEIALGKWLADTYLSHYGHLL 280
           N TT EGF+ DHGHFNT TEI LGKWLA+TYL+HYGHLL
Sbjct: 252 NFTTFEGFSWDHGHFNTETEIVLGKWLANTYLAHYGHLL 288

BLAST of Spg036053 vs. ExPASy TrEMBL
Match: A0A6J1GIT2 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454649 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 8.6e-122
Identity = 211/279 (75.63%), Postives = 241/279 (86.38%), Query Frame = 0

Query: 1   MMLFGPSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRL 60
           M+LF PSLS AT+P NIFILAGQSNMAGRGGVE  ++G KLVWDG VP  C  +PSILRL
Sbjct: 12  MILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNG-KLVWDGKVPLECLSNPSILRL 71

Query: 61  NPQCQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIE 120
           NP+ QWEIA EP+H GID+   TPGIGP +  A Q +EK GQKAG+VGLVPCA+GGTLIE
Sbjct: 72  NPERQWEIAHEPLHLGIDI-SHTPGIGPGIPFAHQFKEKAGQKAGIVGLVPCARGGTLIE 131

Query: 121 QWMKNPSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFF 180
           QWMKNPSNP+ TFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DTA RYKDNLKKF 
Sbjct: 132 QWMKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFI 191

Query: 181 TDVRNDIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPI 240
           TD+RNDIKPRFLP+IIVK +LYDFFMKHDT++LP VRAA++A+Q ELPD++TIDS +LP+
Sbjct: 192 TDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPV 251

Query: 241 NLTTHEGFNLDHGHFNTTTEIALGKWLADTYLSHYGHLL 280
           N TT EGF+ D GHFNT TEI LGKWLADTYL+HYGHLL
Sbjct: 252 NFTTFEGFSWDRGHFNTETEIVLGKWLADTYLAHYGHLL 288

BLAST of Spg036053 vs. ExPASy TrEMBL
Match: A0A6J1DUH7 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111024143 PE=4 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.5e-121
Identity = 216/279 (77.42%), Postives = 243/279 (87.10%), Query Frame = 0

Query: 1   MMLFGPSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRL 60
           MML GPSLSRAT+P NIFILAGQSNMAGRGGVEND+ G  LVWDGYVPP  QPDPSILRL
Sbjct: 12  MMLCGPSLSRATSPKNIFILAGQSNMAGRGGVENDRPG-HLVWDGYVPPEAQPDPSILRL 71

Query: 61  NPQCQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIE 120
           NP+ QWE+AREP+H GID+ +KT G+GPA+A ARQ+Q K G K G VGLVPCA+GGTLIE
Sbjct: 72  NPERQWEVAREPVHEGIDI-NKTVGVGPAIAFARQLQAKGGSKVGSVGLVPCARGGTLIE 131

Query: 121 QWMKNPSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFF 180
           QW+KNPSN + TFY+NFIERI+ASD+EGGVVRALFW  GESDAA  DTA+RYK+NLKKFF
Sbjct: 132 QWVKNPSNTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTANRYKNNLKKFF 191

Query: 181 TDVRNDIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPI 240
           TD+RNDIKPRFLPII+VK  +YD FMKHDT+DLP VRAA++A+Q ELP+VVTIDS KL +
Sbjct: 192 TDIRNDIKPRFLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVVTIDSLKL-V 251

Query: 241 NLTTHEGFNLDHGHFNTTTEIALGKWLADTYLSHYGHLL 280
           N TT EGFNLD GHFN  TEIALGKWLADTYLS YGHLL
Sbjct: 252 NTTTVEGFNLDRGHFNIKTEIALGKWLADTYLSQYGHLL 287

BLAST of Spg036053 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 184.5 bits (467), Expect = 1.2e-46
Identity = 109/273 (39.93%), Postives = 155/273 (56.78%), Query Frame = 0

Query: 6   PSLSRATAPNN--IFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRLNPQ 65
           P L   T   N  IFILAGQSNMAGRGGV ND +    VWDG +PP C+ +PSILRL  +
Sbjct: 18  PHLQSQTITRNISIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSK 77

Query: 66  CQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIEQWM 125
            +W+ A+EP+H  ID+ +KT G+GP M  A ++  + GQ    VGLVPC+ GGT + QW 
Sbjct: 78  LEWKEAKEPLHVDIDI-NKTNGVGPGMPFANRVVNRFGQ----VGLVPCSIGGTKLSQWQ 137

Query: 126 KNPSNPNKTFYQNFIERIKA--SDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFFT 185
           K      +  Y+  ++R KA  +   GG  RA+ W+ GESD      A  YK  L KFF+
Sbjct: 138 K-----GEFLYEETVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFS 197

Query: 186 DVRNDIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEA-LQNELPDVVTIDSWKLPI 245
           D+RND++   LPII V            T   P + A ++A L+ +L +V  +D+  LP+
Sbjct: 198 DLRNDLQHPNLPIIQVALA---------TGAGPYLDAVRKAQLKTDLENVYCVDARGLPL 257

Query: 246 NLTTHEGFNLDHGHFNTTTEIALGKWLADTYLS 274
                     D  H  T++++ LG  +A+++L+
Sbjct: 258 --------EPDGLHLTTSSQVQLGHMIAESFLA 263

BLAST of Spg036053 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 180.3 bits (456), Expect = 2.2e-45
Identity = 101/270 (37.41%), Postives = 146/270 (54.07%), Query Frame = 0

Query: 6   PSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRLNPQCQ 65
           P +     PN IFIL+GQSNMAGRGGV  D    + VWD  +PP C P+ SILRL+   +
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 66  WEIAREPIHAGIDLPDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIEQWMKN 125
           WE A EP+H  ID   K  G+GP MA A  ++ ++   + V+GLVPCA GGT I++W + 
Sbjct: 73  WEEAHEPLHVDID-TGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER- 132

Query: 126 PSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFFTDVRN 185
                   Y+  ++R + S K GG ++A+ W+ GESD      A  Y +N+ +   ++R+
Sbjct: 133 ----GSHLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRH 192

Query: 186 DIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPINLTTH 245
           D+    LPII V        +      +  VR AQ  L  +L +VV +D+  LP+     
Sbjct: 193 DLNLPSLPIIQVA-------IASGGGYIDKVREAQLGL--KLSNVVCVDAKGLPL----- 252

Query: 246 EGFNLDHGHFNTTTEIALGKWLADTYLSHY 276
                D+ H  T  ++ LG  LA  YLS++
Sbjct: 253 ---KSDNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of Spg036053 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 180.3 bits (456), Expect = 2.2e-45
Identity = 101/270 (37.41%), Postives = 146/270 (54.07%), Query Frame = 0

Query: 6   PSLSRATAPNNIFILAGQSNMAGRGGVENDKSGKKLVWDGYVPPVCQPDPSILRLNPQCQ 65
           P +     PN IFIL+GQSNMAGRGGV  D    + VWD  +PP C P+ SILRL+   +
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 66  WEIAREPIHAGIDLPDKTPGIGPAMAIARQIQEKVGQKAGVVGLVPCAKGGTLIEQWMKN 125
           WE A EP+H  ID   K  G+GP MA A  ++ ++   + V+GLVPCA GGT I++W + 
Sbjct: 73  WEEAHEPLHVDID-TGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER- 132

Query: 126 PSNPNKTFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHRYKDNLKKFFTDVRN 185
                   Y+  ++R + S K GG ++A+ W+ GESD      A  Y +N+ +   ++R+
Sbjct: 133 ----GSHLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRH 192

Query: 186 DIKPRFLPIIIVKATLYDFFMKHDTNDLPTVRAAQEALQNELPDVVTIDSWKLPINLTTH 245
           D+    LPII V        +      +  VR AQ  L  +L +VV +D+  LP+     
Sbjct: 193 DLNLPSLPIIQVA-------IASGGGYIDKVREAQLGL--KLSNVVCVDAKGLPL----- 252

Query: 246 EGFNLDHGHFNTTTEIALGKWLADTYLSHY 276
                D+ H  T  ++ LG  LA  YLS++
Sbjct: 253 ---KSDNLHLTTEAQVQLGLSLAQAYLSNF 259