Spg035936 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg035936 vs. NCBI nr
Match: XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 677.9 bits (1748), Expect = 7.9e-191
Identity = 328/454 (72.25%), Postives = 379/454 (83.48%), Query Frame = 0

Query: 145 IITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSLPFIVIDLINLTTISIDHK 204
           IITPL VSH+Q AV+CS+ + LQIR RSGGHD+EGLS +S LPFI+IDLINL +I+ID  
Sbjct: 87  IITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLSCVSHLPFIIIDLINLRSITIDAN 146

Query: 205 NNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAADN 264
           NNTAWVQSGAT+GELYY I + S TLAFPAG CPTVG+GG FSGGG G + RK+GLAADN
Sbjct: 147 NNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVGIGGHFSGGGYGLMLRKFGLAADN 206

Query: 265 VIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNVT 324
           VIDAYLVDANGKF DRE MGEDLFWAIRGGGGGSFGIVVAWKVKLV VP  VTSC  N T
Sbjct: 207 VIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTSCYYNRT 266

Query: 325 LEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTL----GAKTNPTASFTSLFLGKANELM 384
           LEE A+KLIHRWQYVANK+DENLFL I+L GG      G KTNPTASF SLFLGKA+E  
Sbjct: 267 LEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQGGDKTNPTASFFSLFLGKADEFF 326

Query: 385 AILNTTFPELSLAKKDCIESSWINSTLTIAGFTPGEALETLLNRKSPTTKSTKVKSDYVK 444
           AILNTTFPEL LAK++C+ESSWI S L  AGF  G++LE LLNR   T  S K+KSDYVK
Sbjct: 327 AILNTTFPELGLAKENCLESSWIESALLSAGFKIGDSLEALLNRTPLTNGSIKIKSDYVK 386

Query: 445 EPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIKW 504
           EPISEA I+G+W+RLK+RD+   +LLF+PYGGRMSQI ESE P+SHR G LY+IGY + W
Sbjct: 387 EPISEATIEGIWERLKSRDVGARQLLFVPYGGRMSQISESEIPFSHRAGNLYKIGYIVAW 446

Query: 505 QEESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASVWG 564
           Q++++D EKR + W+R+L+ YMTPFVSK PR++Y NYRDLD+G+NNKYG TS+K+ASVWG
Sbjct: 447 QKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYVNYRDLDIGSNNKYGKTSFKRASVWG 506

Query: 565 LNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPILS 595
             YFGNNF+RLVHVKTKVDPY+FF HEQSIP +S
Sbjct: 507 WKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTIS 540

BLAST of Spg035936 vs. NCBI nr
Match: XP_022142587.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 675.6 bits (1742), Expect = 3.9e-190
Identity = 329/455 (72.31%), Postives = 381/455 (83.74%), Query Frame = 0

Query: 144 VIITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSLPFIVIDLINLTTISIDH 203
           +I+TP  VSHIQ AVICSK    QIR RSGGHD+EGLSY++ LPFI++DLINL +ISID 
Sbjct: 85  LIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYVAHLPFIIVDLINLRSISIDI 144

Query: 204 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 263
           K+NTAWVQSGAT+GELYY I + S TLAFPAG+CPTVGVGG FSGGG G L RKYGLAAD
Sbjct: 145 KDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVGGHFSGGGYGLLLRKYGLAAD 204

Query: 264 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 323
           NVIDAYLVDA+GKFH+RESMGEDLFWAIRGGGGGSFGIVVAWKVKLV VP  VT C  N 
Sbjct: 205 NVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCSANR 264

Query: 324 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTL----GAKTNPTASFTSLFLGKANEL 383
           TLEE AVK+ HRWQYVANK+DENLFL I+  GG +    G KTNP A+F SLFLGKA+EL
Sbjct: 265 TLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGGKTNPVATFFSLFLGKADEL 324

Query: 384 MAILNTTFPELSLAKKDCIESSWINSTLTI-AGFTPGEALETLLNRKSPTTKSTKVKSDY 443
           ++ILNT FPEL LAKKDCIE+SWI ST+ +  GF     LE LL+R   T  S+K+KSDY
Sbjct: 325 LSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTLEALLSRTPLTNTSSKIKSDY 384

Query: 444 VKEPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKI 503
           VKEP+SEA ++G+WKRL ++DIE   ++F+PYGGRMSQI ESETP+SHR GYLY+IGY  
Sbjct: 385 VKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQISESETPFSHRAGYLYKIGYIA 444

Query: 504 KWQEESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASV 563
            W+E+ IDAEKR + W+R+L+DYMTPFVSK PRA+Y+NYRDLD+G+NNK+G TSYKQASV
Sbjct: 445 GWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANYRDLDIGSNNKFGKTSYKQASV 504

Query: 564 WGLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPIL 594
           WGL YFGNNF+RLVHVKTKVDPY+FF HEQSIP L
Sbjct: 505 WGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of Spg035936 vs. NCBI nr
Match: XP_023520318.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 669.8 bits (1727), Expect = 2.1e-188
Identity = 320/453 (70.64%), Postives = 385/453 (84.99%), Query Frame = 0

Query: 144 VIITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSLPFIVIDLINLTTISIDH 203
           +IITP  +SHIQ AVICSK + LQIR RSGGHD+EGLSY++  PFI++DLINL +I++D 
Sbjct: 80  LIITPSHISHIQAAVICSKAHDLQIRTRSGGHDFEGLSYVADQPFIIVDLINLRSINVDV 139

Query: 204 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 263
           + NTAWVQSGAT+GELYY+I + S +LAFPAG+CPTVGVGG FSGGG G + RKYGLAAD
Sbjct: 140 QTNTAWVQSGATLGELYYRIGEKSRSLAFPAGICPTVGVGGHFSGGGYGLMLRKYGLAAD 199

Query: 264 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 323
           NV+DAYLVDANG+ HDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV VP +VT C  N 
Sbjct: 200 NVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVVVPAVVTMCSTNR 259

Query: 324 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTLGAK---TNPTASFTSLFLGKANELM 383
            +E+DA+KLIHRWQYVAN++D+NLFL I+LTGG    +   TNPTA+F SL+LGK +EL+
Sbjct: 260 DVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTRQGITNPTATFFSLYLGKLDELI 319

Query: 384 AILNTTFPELSLAKKDCIESSWINSTLTIA-GFTPGEALETLLNRKSPTTKSTKVKSDYV 443
           AIL TTFPEL L KKDC+ESSWI STL  + G    E LE LLNR  PT +STK KSDYV
Sbjct: 320 AILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLNRTPPTIESTKFKSDYV 379

Query: 444 KEPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIK 503
           K+PI EAAI+G+W+RLKA+DIEV ++LFIPYGGRMSQI ESETP+SHR G LY+IGY + 
Sbjct: 380 KDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETPFSHRAGNLYKIGYAVS 439

Query: 504 WQEESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASVW 563
           W+E+S+DA+ R +NW+R++ ++MTPFVSK PRA+Y NYRDLD+GTNNKYG TSY+QASVW
Sbjct: 440 WKEQSLDAKNRHINWIREVNEHMTPFVSKSPRAAYVNYRDLDIGTNNKYGKTSYEQASVW 499

Query: 564 GLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPI 593
           G+ YFGNNF+RLV+VKTKVDP++FF HEQSIP+
Sbjct: 500 GMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIPV 532

BLAST of Spg035936 vs. NCBI nr
Match: KAG6583829.1 (Berberine bridge enzyme-like 26, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 667.9 bits (1722), Expect = 8.1e-188
Identity = 318/453 (70.20%), Postives = 384/453 (84.77%), Query Frame = 0

Query: 144 VIITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSLPFIVIDLINLTTISIDH 203
           +IITP  +SHIQ AVICSK + LQIR RSGGHD+EGLSY++  PFI++DLINL +I++D 
Sbjct: 80  LIITPSHISHIQAAVICSKAHDLQIRTRSGGHDFEGLSYVADQPFIIVDLINLRSINVDV 139

Query: 204 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 263
           + NTAWVQSGAT+GELYY+I + S +LAFPAG+CPTVGVGG FSGGG G + RKYGLAAD
Sbjct: 140 QTNTAWVQSGATLGELYYRIGEKSRSLAFPAGICPTVGVGGHFSGGGYGLMLRKYGLAAD 199

Query: 264 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 323
           NV+DAYLVDANG+ HDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV VP +VT C  N 
Sbjct: 200 NVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVVVPAVVTMCSTNR 259

Query: 324 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTLGAK---TNPTASFTSLFLGKANELM 383
            +E+DA+KLIHRWQYVAN++D++LFL I+LTGG    +   TNPTA+F SL+LGK +EL+
Sbjct: 260 DVEDDAIKLIHRWQYVANQLDDDLFLGILLTGGNFSTREGITNPTATFFSLYLGKLDELI 319

Query: 384 AILNTTFPELSLAKKDCIESSWINSTLTIA-GFTPGEALETLLNRKSPTTKSTKVKSDYV 443
           AIL TTFPEL L KKDC+ESSWI STL  + G    E LE LLNR  PT +STK KSDYV
Sbjct: 320 AILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLNRTPPTIESTKFKSDYV 379

Query: 444 KEPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIK 503
           K+PI EAAI+G+W+RLKA+DIEV ++LFIPYGGRMSQI ESETP+SHR G LY+IGY + 
Sbjct: 380 KDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETPFSHRAGNLYKIGYAVS 439

Query: 504 WQEESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASVW 563
           W+E+S+DA+ R +NW+R++++YM PFVSK PRA+Y NYRDLD+GTNNKYG TSY+QASVW
Sbjct: 440 WKEQSLDAKNRHINWIREVYEYMAPFVSKSPRAAYVNYRDLDIGTNNKYGKTSYEQASVW 499

Query: 564 GLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPI 593
           G+ YFGNNF+RLV+VKT VDP++FF HEQSIP+
Sbjct: 500 GMKYFGNNFNRLVYVKTMVDPHDFFRHEQSIPV 532

BLAST of Spg035936 vs. NCBI nr
Match: XP_023001021.1 (berberine bridge enzyme-like 18 [Cucurbita maxima])

HSP 1 Score: 666.8 bits (1719), Expect = 1.8e-187
Identity = 314/453 (69.32%), Postives = 384/453 (84.77%), Query Frame = 0

Query: 144 VIITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSLPFIVIDLINLTTISIDH 203
           +IITP  +SHIQ A++CSK + LQIR RSGGHD+EGLSY+++ PFI++DLINL +I++D 
Sbjct: 80  LIITPSHISHIQAAIVCSKAHDLQIRTRSGGHDFEGLSYVANQPFIIVDLINLRSINVDV 139

Query: 204 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 263
           + NTAWVQSGAT+GELYY+I +TS +LAFPAG+CPTVGVGG FSGGG G + RKYGLAAD
Sbjct: 140 QTNTAWVQSGATLGELYYRIGETSRSLAFPAGICPTVGVGGHFSGGGYGLMLRKYGLAAD 199

Query: 264 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 323
           NV+DAYLVDANG+ HDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV VP +VT C  N 
Sbjct: 200 NVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVMVPAVVTMCSTNR 259

Query: 324 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTLGAK---TNPTASFTSLFLGKANELM 383
            +E+DA+KLIHRWQYVAN++D+NLFL I+LTGG    +   TNPTA+F SL+LGK +EL+
Sbjct: 260 DVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTREGITNPTATFFSLYLGKLDELI 319

Query: 384 AILNTTFPELSLAKKDCIESSWINSTLTIA-GFTPGEALETLLNRKSPTTKSTKVKSDYV 443
            IL   F EL L KKDC+ESSWI STL  + G    E LE LLNR  PT +STK KSDYV
Sbjct: 320 TILKNNFSELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLNRTPPTIESTKFKSDYV 379

Query: 444 KEPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIK 503
           K+PI EA I+G+W+RLKA+DIEV ++LFIPYGGRMSQI ESETP+SHR G LY+IGY + 
Sbjct: 380 KDPIPEATIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETPFSHRAGNLYKIGYAVS 439

Query: 504 WQEESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASVW 563
           W+E+S+DA+ + +NW+R++++YMTPFVSK PRA+Y+NYRDLD+GTNNKYG TSY+QASVW
Sbjct: 440 WKEQSLDAKNKHINWIREVYEYMTPFVSKSPRAAYANYRDLDIGTNNKYGKTSYEQASVW 499

Query: 564 GLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPI 593
           G+ YFGNNF+RLV+VKTKVDP++FF HEQSIP+
Sbjct: 500 GMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIPV 532

BLAST of Spg035936 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 3.3e-131
Identity = 234/454 (51.54%), Postives = 315/454 (69.38%), Query Frame = 0

Query: 145 IITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSL--PFIVIDLINLTTISID 204
           I +P+  SH+Q ++ICSKK  + +R+RSGGHDYEGLSY+S +  PFI++DL  +  ++I+
Sbjct: 87  IFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNIN 146

Query: 205 HKNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAA 264
            ++N+AWVQSGATVGELYY+I + S    FPAG+C ++G+GG  +GG  G++ RKYGL A
Sbjct: 147 IQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGA 206

Query: 265 DNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLN 324
           DNV+DA +VDANGK  DR +MGED FWAIRGG GGSFGI++AWK+KLV VP+ VT   + 
Sbjct: 207 DNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVT 266

Query: 325 VTLEED-AVKLIHRWQYVANKVDENLFLAIVLTGGTLGAKTNPTASFTSLFLGKANELMA 384
            TL++D   K+I +WQ VA+K+ E LF+ ++      G     T S+ +LFLG    LM 
Sbjct: 267 KTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNKTVTTSYNALFLGGKGTLMN 326

Query: 385 ILNTTFPELSLAKKDCIESSWINSTLTIAGFTPGEALETLLNRKSPTTK-STKVKSDYVK 444
           ++  +FPEL L  KDCIE SW+ S   I+GF        LL  KSP  K S K KSD+VK
Sbjct: 327 VMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVK 386

Query: 445 EPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIKW 504
            PI E+ ++G++K+L   DI +  +++ PYGG M++I ES+ P+ HRKG L+++ Y   W
Sbjct: 387 TPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSW 446

Query: 505 QEESIDAEK---RQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQAS 564
               +D++K   R +NW+R L+ YMTP+VS  PR +Y NYRDLDLG N K   T  KQA 
Sbjct: 447 ----LDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDVKTCIKQAQ 506

Query: 565 VWGLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIP 592
           VWG NYF NNF+RL+ +K KVDP NFF HEQSIP
Sbjct: 507 VWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534

BLAST of Spg035936 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 6.2e-130
Identity = 235/458 (51.31%), Postives = 322/458 (70.31%), Query Frame = 0

Query: 145 IITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLS-SLPFIVIDLINLTTISIDH 204
           IITP+Q + +Q  + C++ + + IR RSGGHDYEGLSY++ S PF+VIDL NL +I++D 
Sbjct: 85  IITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDV 144

Query: 205 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 264
            N T WVQSGAT+GELYY+I K S +LAFPAG+ PTVG+GG F GGG GTL RKYGL+AD
Sbjct: 145 DNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSAD 204

Query: 265 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 324
           NVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V++WK++L+ VP +VT   +  
Sbjct: 205 NVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVK 264

Query: 325 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTLGAKTNPTASFTSLFLGKANELMAIL 384
           T E++AV +I++WQY+A+KV  +LF+  +L       +T   ASF  L+LG  ++L+A++
Sbjct: 265 TSEKEAVSIINKWQYIADKVPNDLFIRAMLQ-----KETEVYASFPGLYLGPVSDLLALM 324

Query: 385 NTTFPELSLAKKDCIESSWINSTLTIAGFTPGEALETLLNRKSPTTKSTKVKSDYVKEPI 444
              FPEL L   +C E SWI S L    F  GE++E L  RK  T++S K K D+++EPI
Sbjct: 325 KDKFPELGLEIGNCREMSWIESVL---WFIKGESMEILAKRKR-TSRSFKGKDDFIEEPI 384

Query: 445 SEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIKWQEE 504
            + AI+ LW+R +A +  + K++  P+GG+MS+I ++E P+ HR+G LY I Y   W EE
Sbjct: 385 PKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEE 444

Query: 505 SIDAEK----RQVNWMRKLFDYMTPFVSKLPRASYSNYRDLD----LGTNNKYGATSYKQ 564
             D  K    + + W+  ++++MTP+VSK PR +Y N+RD+D    LG N K   T Y++
Sbjct: 445 E-DKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMK---TKYEE 504

Query: 565 ASVWGLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPIL 594
           A VWG+ YF NNFDRLV VKT VDP +FF  EQSIPI+
Sbjct: 505 AKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529

BLAST of Spg035936 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 4.0e-129
Identity = 232/458 (50.66%), Postives = 319/458 (69.65%), Query Frame = 0

Query: 145 IITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLS-SLPFIVIDLINLTTISIDH 204
           IITP++ S +Q  + C++ + + +R RS GH YEGLSY++ + PF VIDL NL +IS+D 
Sbjct: 81  IITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDV 140

Query: 205 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 264
            N T WVQ+GAT GELYY+I KT+ +LAFPAG+ PTVGVGG FSGGG GTL RKYGLAAD
Sbjct: 141 DNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAAD 200

Query: 265 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 324
           N+IDA +VDA+G+  DR++MGED FWAIRGGGG SFG++++WKVKLV VP  +T   +  
Sbjct: 201 NIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQK 260

Query: 325 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTLGAKTNPTASFTSLFLGKANELMAIL 384
           T +++AV++I +WQY A+KV ++LF+   L       K    A FT L++G  N L+A++
Sbjct: 261 TSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN---KNAVHALFTGLYIGPVNNLLALM 320

Query: 385 NTTFPELSLAKKDCIESSWINSTLTIAGFTPGEALETLLNRKSPTTKSTKVKSDYVKEPI 444
              FPEL L K+ C E SWI S L  A F  GE+L  L NR+  T+ S K K D+V+EPI
Sbjct: 321 EEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLTNRER-TSLSFKGKDDFVQEPI 380

Query: 445 SEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIKWQEE 504
            EAAI+ +W+RL+A +  + K++  P+GG+MS++ E ETP+ HR G LY I Y   W+EE
Sbjct: 381 PEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREE 440

Query: 505 SIDAEK----RQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASVW 564
             D  K    + + W+  ++++MTP+VSK PR +Y N++D+DLG       T Y++   W
Sbjct: 441 E-DKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKKTKYEEGKSW 500

Query: 565 GLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPILSSNT 598
           G+ YF NNF+RLV VKT+VDP +FF  EQSIP+++  T
Sbjct: 501 GVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVNKVT 533

BLAST of Spg035936 vs. ExPASy Swiss-Prot
Match: Q9SUC6 (Berberine bridge enzyme-like 22 OS=Arabidopsis thaliana OX=3702 GN=FAD-OXR PE=2 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 8.3e-127
Identity = 226/451 (50.11%), Postives = 308/451 (68.29%), Query Frame = 0

Query: 144 VIITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSLPFIVIDLINLTTISIDH 203
           +I+ P   S I+ +++CS+K  +Q+R  SGGHDYEGLSYLS  PFI++DL+NL +ISI+ 
Sbjct: 83  LIVKPKSESEIKQSILCSRKLGVQVRTMSGGHDYEGLSYLSLSPFIIVDLVNLRSISINL 142

Query: 204 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 263
            + TAW+QSGAT+GE+YYKI KTS   AF AG+CP+VGVGG  SGGG GT+ RKYGLA+D
Sbjct: 143 TDETAWIQSGATLGEVYYKIAKTSKIHAFAAGICPSVGVGGHISGGGFGTIMRKYGLASD 202

Query: 264 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 323
           NV+DA L+D NGK  DR++MGEDLFWA+RGGG  SFG+V++WKVKL RVP  VT  +   
Sbjct: 203 NVVDARLMDVNGKTLDRKTMGEDLFWALRGGGAASFGVVLSWKVKLARVPEKVTCFISQH 262

Query: 324 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTLGAKTNPTASFTSLFLGKANELMAIL 383
            +     KL+HRWQ + +++DE+LF+ +++     G +    ++F +LFLG  + L+ ++
Sbjct: 263 PMGPSMNKLVHRWQSIGSELDEDLFIRVIIDNSLEGNQRKVKSTFQTLFLGGIDRLIPLM 322

Query: 384 NTTFPELSLAKKDCIESSWINSTLTIAGFTPGEALETLLNRKSP-TTKSTKVKSDYVKEP 443
           N  FPEL L  +DC E SWI S +    +  G+ LE LLNR      +  K KSDYV++P
Sbjct: 323 NQKFPELGLRSQDCSEMSWIES-IMFFNWRSGQPLEILLNRDLRFEDQYFKAKSDYVQKP 382

Query: 444 ISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIKWQE 503
           + E   + + KR   +D  +  ++F P GG++S+I E+E+PY HR+G LY I Y +KW+ 
Sbjct: 383 VPENVFEEVTKRFLEQDTPL--MIFEPLGGKISKISETESPYPHRRGNLYNIQYMVKWKV 442

Query: 504 ESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASVWGLN 563
             ++   + V WMR L DYMTP+VSK PR +Y NYRDLDLG+      TS++ A  WG  
Sbjct: 443 NEVEEMNKHVRWMRSLHDYMTPYVSKSPRGAYLNYRDLDLGSTKGIN-TSFEDARKWGET 502

Query: 564 YFGNNFDRLVHVKTKVDPYNFFMHEQSIPIL 594
           YF  NF RL  VK K+DP NFF +EQSIP L
Sbjct: 503 YFKGNFKRLGLVKGKIDPTNFFRNEQSIPPL 529

BLAST of Spg035936 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 1.6e-125
Identity = 223/454 (49.12%), Postives = 312/454 (68.72%), Query Frame = 0

Query: 143 EVIITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSL--PFIVIDLINLTTIS 202
           E I  PL  +H+Q AV+C+KK  L +R+RSGGHDYEGLSY+S +   F+++DL  L  IS
Sbjct: 85  EFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVDLSKLRQIS 144

Query: 203 IDHKNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGL 262
           +D ++N+AWV +GA++GE+YY+I + S    FPAG+C ++G+GG   GG  G++ RK+GL
Sbjct: 145 VDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMMRKFGL 204

Query: 263 AADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCL 322
            ADNV+DA +VDA+GK  +R +MGED+FWAIRGGGGGSFG+++AWK+KLV VP IVT   
Sbjct: 205 GADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEIVTVFT 264

Query: 323 LNVTLEEDAVKLIHRWQYVANKVDENLFLAIVL--TGGTLGAKTNP-TASFTSLFLGKAN 382
           +  TLE+D  KL+++WQ VA+K+DE+LF+ +++  T  T  +K    + S+   FLG AN
Sbjct: 265 VTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTISTSYQGQFLGDAN 324

Query: 383 ELMAILNTTFPELSLAKKDCIESSWINSTLTIAGFTPGEALETLLNRKSPTTKSTKVKSD 442
            L+ ++  +FP+L L KKDC+E+SWI S + IAGF      E LL+ KS      K KSD
Sbjct: 325 RLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKSLFKNYFKAKSD 384

Query: 443 YVKEPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYK 502
           YV+EPI    ++GLW++L   D  +   ++ PYGG M++I E+ETP+ HR G L++I + 
Sbjct: 385 YVEEPIPVEGLEGLWEKLLEEDSPL--TIWNPYGGMMAKIPETETPFPHRSGTLFKIQWL 444

Query: 503 IKWQEESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQAS 562
             WQ+    +E + + WMR+++ YM  +VSK PR++Y NYRDLDLG N K        A 
Sbjct: 445 TLWQDGK-TSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGK-----GSDAR 504

Query: 563 VWGLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIP 592
            WG  YF  NF+RLV +K K DP NFF HEQSIP
Sbjct: 505 EWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Spg035936 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 3.8e-191
Identity = 328/454 (72.25%), Postives = 379/454 (83.48%), Query Frame = 0

Query: 145 IITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSLPFIVIDLINLTTISIDHK 204
           IITPL VSH+Q AV+CS+ + LQIR RSGGHD+EGLS +S LPFI+IDLINL +I+ID  
Sbjct: 87  IITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLSCVSHLPFIIIDLINLRSITIDAN 146

Query: 205 NNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAADN 264
           NNTAWVQSGAT+GELYY I + S TLAFPAG CPTVG+GG FSGGG G + RK+GLAADN
Sbjct: 147 NNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVGIGGHFSGGGYGLMLRKFGLAADN 206

Query: 265 VIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNVT 324
           VIDAYLVDANGKF DRE MGEDLFWAIRGGGGGSFGIVVAWKVKLV VP  VTSC  N T
Sbjct: 207 VIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTSCYYNRT 266

Query: 325 LEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTL----GAKTNPTASFTSLFLGKANELM 384
           LEE A+KLIHRWQYVANK+DENLFL I+L GG      G KTNPTASF SLFLGKA+E  
Sbjct: 267 LEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQGGDKTNPTASFFSLFLGKADEFF 326

Query: 385 AILNTTFPELSLAKKDCIESSWINSTLTIAGFTPGEALETLLNRKSPTTKSTKVKSDYVK 444
           AILNTTFPEL LAK++C+ESSWI S L  AGF  G++LE LLNR   T  S K+KSDYVK
Sbjct: 327 AILNTTFPELGLAKENCLESSWIESALLSAGFKIGDSLEALLNRTPLTNGSIKIKSDYVK 386

Query: 445 EPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIKW 504
           EPISEA I+G+W+RLK+RD+   +LLF+PYGGRMSQI ESE P+SHR G LY+IGY + W
Sbjct: 387 EPISEATIEGIWERLKSRDVGARQLLFVPYGGRMSQISESEIPFSHRAGNLYKIGYIVAW 446

Query: 505 QEESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASVWG 564
           Q++++D EKR + W+R+L+ YMTPFVSK PR++Y NYRDLD+G+NNKYG TS+K+ASVWG
Sbjct: 447 QKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYVNYRDLDIGSNNKYGKTSFKRASVWG 506

Query: 565 LNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPILS 595
             YFGNNF+RLVHVKTKVDPY+FF HEQSIP +S
Sbjct: 507 WKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTIS 540

BLAST of Spg035936 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.9e-190
Identity = 329/455 (72.31%), Postives = 381/455 (83.74%), Query Frame = 0

Query: 144 VIITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSLPFIVIDLINLTTISIDH 203
           +I+TP  VSHIQ AVICSK    QIR RSGGHD+EGLSY++ LPFI++DLINL +ISID 
Sbjct: 85  LIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYVAHLPFIIVDLINLRSISIDI 144

Query: 204 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 263
           K+NTAWVQSGAT+GELYY I + S TLAFPAG+CPTVGVGG FSGGG G L RKYGLAAD
Sbjct: 145 KDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVGGHFSGGGYGLLLRKYGLAAD 204

Query: 264 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 323
           NVIDAYLVDA+GKFH+RESMGEDLFWAIRGGGGGSFGIVVAWKVKLV VP  VT C  N 
Sbjct: 205 NVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCSANR 264

Query: 324 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTL----GAKTNPTASFTSLFLGKANEL 383
           TLEE AVK+ HRWQYVANK+DENLFL I+  GG +    G KTNP A+F SLFLGKA+EL
Sbjct: 265 TLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGGKTNPVATFFSLFLGKADEL 324

Query: 384 MAILNTTFPELSLAKKDCIESSWINSTLTI-AGFTPGEALETLLNRKSPTTKSTKVKSDY 443
           ++ILNT FPEL LAKKDCIE+SWI ST+ +  GF     LE LL+R   T  S+K+KSDY
Sbjct: 325 LSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTLEALLSRTPLTNTSSKIKSDY 384

Query: 444 VKEPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKI 503
           VKEP+SEA ++G+WKRL ++DIE   ++F+PYGGRMSQI ESETP+SHR GYLY+IGY  
Sbjct: 385 VKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQISESETPFSHRAGYLYKIGYIA 444

Query: 504 KWQEESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASV 563
            W+E+ IDAEKR + W+R+L+DYMTPFVSK PRA+Y+NYRDLD+G+NNK+G TSYKQASV
Sbjct: 445 GWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANYRDLDIGSNNKFGKTSYKQASV 504

Query: 564 WGLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPIL 594
           WGL YFGNNF+RLVHVKTKVDPY+FF HEQSIP L
Sbjct: 505 WGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of Spg035936 vs. ExPASy TrEMBL
Match: A0A6J1KHF6 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111495284 PE=3 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 8.8e-188
Identity = 314/453 (69.32%), Postives = 384/453 (84.77%), Query Frame = 0

Query: 144 VIITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSLPFIVIDLINLTTISIDH 203
           +IITP  +SHIQ A++CSK + LQIR RSGGHD+EGLSY+++ PFI++DLINL +I++D 
Sbjct: 80  LIITPSHISHIQAAIVCSKAHDLQIRTRSGGHDFEGLSYVANQPFIIVDLINLRSINVDV 139

Query: 204 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 263
           + NTAWVQSGAT+GELYY+I +TS +LAFPAG+CPTVGVGG FSGGG G + RKYGLAAD
Sbjct: 140 QTNTAWVQSGATLGELYYRIGETSRSLAFPAGICPTVGVGGHFSGGGYGLMLRKYGLAAD 199

Query: 264 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 323
           NV+DAYLVDANG+ HDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV VP +VT C  N 
Sbjct: 200 NVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVMVPAVVTMCSTNR 259

Query: 324 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTLGAK---TNPTASFTSLFLGKANELM 383
            +E+DA+KLIHRWQYVAN++D+NLFL I+LTGG    +   TNPTA+F SL+LGK +EL+
Sbjct: 260 DVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTREGITNPTATFFSLYLGKLDELI 319

Query: 384 AILNTTFPELSLAKKDCIESSWINSTLTIA-GFTPGEALETLLNRKSPTTKSTKVKSDYV 443
            IL   F EL L KKDC+ESSWI STL  + G    E LE LLNR  PT +STK KSDYV
Sbjct: 320 TILKNNFSELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLNRTPPTIESTKFKSDYV 379

Query: 444 KEPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIK 503
           K+PI EA I+G+W+RLKA+DIEV ++LFIPYGGRMSQI ESETP+SHR G LY+IGY + 
Sbjct: 380 KDPIPEATIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETPFSHRAGNLYKIGYAVS 439

Query: 504 WQEESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASVW 563
           W+E+S+DA+ + +NW+R++++YMTPFVSK PRA+Y+NYRDLD+GTNNKYG TSY+QASVW
Sbjct: 440 WKEQSLDAKNKHINWIREVYEYMTPFVSKSPRAAYANYRDLDIGTNNKYGKTSYEQASVW 499

Query: 564 GLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPI 593
           G+ YFGNNF+RLV+VKTKVDP++FF HEQSIP+
Sbjct: 500 GMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIPV 532

BLAST of Spg035936 vs. ExPASy TrEMBL
Match: A0A6J1CKW8 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 PE=3 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 8.2e-186
Identity = 326/455 (71.65%), Postives = 371/455 (81.54%), Query Frame = 0

Query: 144 VIITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSLPFIVIDLINLTTISIDH 203
           +IITP  VSHIQ AV+CSK+   QIR RSGGHD+E LSY++ L FI++DLINL +IS+D 
Sbjct: 86  LIITPSHVSHIQAAVVCSKRYGFQIRTRSGGHDFERLSYVAHLSFIIVDLINLRSISVDV 145

Query: 204 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 263
           KNN AWV SGAT+GELYY I + S T AFPAGVCPTVGVGG FSGGG G L RKYGLAAD
Sbjct: 146 KNNNAWVHSGATLGELYYSIAQKSRTSAFPAGVCPTVGVGGHFSGGGYGLLLRKYGLAAD 205

Query: 264 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 323
           NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV VP  VT C  N 
Sbjct: 206 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCQTNR 265

Query: 324 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTL----GAKTNPTASFTSLFLGKANEL 383
           TLEE AVKL HRWQYVANK+DENLFL I+  GG +    G KTNP A+F SLFLG+A+EL
Sbjct: 266 TLEEGAVKLTHRWQYVANKLDENLFLGILWLGGNVTSQGGGKTNPVATFFSLFLGQADEL 325

Query: 384 MAILNTTFPELSLAKKDCIESSWINSTLTIA-GFTPGEALETLLNRKSPTTKSTKVKSDY 443
           +AILNT FPEL LAKKDCIE+SWI ST+ +  GF     LE LL+R   T  STK+KSDY
Sbjct: 326 LAILNTKFPELDLAKKDCIETSWIESTVLMGIGFQTKVTLEALLSRTPLTNISTKIKSDY 385

Query: 444 VKEPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKI 503
           VKEPISEA I+G+ +RL A+DIE   L+F+PYGGRMSQIFESETP+SHR GYLY+IGY  
Sbjct: 386 VKEPISEATIQGIGERLNAQDIESGNLIFVPYGGRMSQIFESETPFSHRAGYLYKIGYIT 445

Query: 504 KWQEESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASV 563
            W ++SID EKR ++W+R+L+ YM PFVSK PRA Y+NYRDLD+G+N +YG TSYKQAS 
Sbjct: 446 SWLDQSIDTEKRHLSWIRELYSYMAPFVSKSPRAVYANYRDLDIGSNKRYGKTSYKQAST 505

Query: 564 WGLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPIL 594
           WG  YFGNNF+RLVHVKTKVDPY+FF HEQSIP L
Sbjct: 506 WGFKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 540

BLAST of Spg035936 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 2.2e-183
Identity = 317/456 (69.52%), Postives = 382/456 (83.77%), Query Frame = 0

Query: 144 VIITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSLPFIVIDLINLTTISIDH 203
           +IITP  VSH+Q AVICSK + LQIRIRSGGHD EGLSY++ LPFI+IDLINL +I+++ 
Sbjct: 82  LIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSYVAHLPFIIIDLINLKSITVNI 141

Query: 204 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 263
           +N+TAW+QSGAT+GELYY I + S TLAFPAGVCPTVGVGG FSGGG G + RKYGLAAD
Sbjct: 142 ENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGVGGHFSGGGYGLMLRKYGLAAD 201

Query: 264 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 323
           NVIDAYL+DANGKF+DRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV VP IVT+C  + 
Sbjct: 202 NVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPAIVTTCSSDR 261

Query: 324 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTLGA-----KTNPTASFTSLFLGKANE 383
            L+ED + LIHRWQ VA K+DENL+  I+L GG         K NPTA+F SLFLGK++E
Sbjct: 262 NLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGGVKKPNPTATFFSLFLGKSDE 321

Query: 384 LMAILNTTFPELSLAKKDCIESSWINSTLTIAGFTPG-EALETLLNRKSPTTKSTKVKSD 443
           LMAILNT FPEL+LAKKDC+E+SWI STL  A      E+LE+LLNR   T ++TK+KSD
Sbjct: 322 LMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIESLESLLNRTPLTIENTKLKSD 381

Query: 444 YVKEPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYK 503
           YVKEPI +A I+G+W+RLKA+DIEV +L+F+PYGGRMS+I +SETP+SHR   LY+IGY 
Sbjct: 382 YVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSRISDSETPFSHRARNLYKIGYI 441

Query: 504 IKWQEESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQAS 563
           + W+E+S+ AE+R ++W+R+++DYMTPFVSK PRA+Y NYRDLD+GTNNKYG TSYKQAS
Sbjct: 442 VGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVNYRDLDIGTNNKYGKTSYKQAS 501

Query: 564 VWGLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPIL 594
           VWG  YFGNNF+RLVH+K+KVDP NFF +EQSIP L
Sbjct: 502 VWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537

BLAST of Spg035936 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 470.3 bits (1209), Expect = 2.3e-132
Identity = 234/454 (51.54%), Postives = 315/454 (69.38%), Query Frame = 0

Query: 145 IITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSL--PFIVIDLINLTTISID 204
           I +P+  SH+Q ++ICSKK  + +R+RSGGHDYEGLSY+S +  PFI++DL  +  ++I+
Sbjct: 87  IFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNIN 146

Query: 205 HKNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAA 264
            ++N+AWVQSGATVGELYY+I + S    FPAG+C ++G+GG  +GG  G++ RKYGL A
Sbjct: 147 IQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGA 206

Query: 265 DNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLN 324
           DNV+DA +VDANGK  DR +MGED FWAIRGG GGSFGI++AWK+KLV VP+ VT   + 
Sbjct: 207 DNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVT 266

Query: 325 VTLEED-AVKLIHRWQYVANKVDENLFLAIVLTGGTLGAKTNPTASFTSLFLGKANELMA 384
            TL++D   K+I +WQ VA+K+ E LF+ ++      G     T S+ +LFLG    LM 
Sbjct: 267 KTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNKTVTTSYNALFLGGKGTLMN 326

Query: 385 ILNTTFPELSLAKKDCIESSWINSTLTIAGFTPGEALETLLNRKSPTTK-STKVKSDYVK 444
           ++  +FPEL L  KDCIE SW+ S   I+GF        LL  KSP  K S K KSD+VK
Sbjct: 327 VMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVK 386

Query: 445 EPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIKW 504
            PI E+ ++G++K+L   DI +  +++ PYGG M++I ES+ P+ HRKG L+++ Y   W
Sbjct: 387 TPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSW 446

Query: 505 QEESIDAEK---RQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQAS 564
               +D++K   R +NW+R L+ YMTP+VS  PR +Y NYRDLDLG N K   T  KQA 
Sbjct: 447 ----LDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDVKTCIKQAQ 506

Query: 565 VWGLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIP 592
           VWG NYF NNF+RL+ +K KVDP NFF HEQSIP
Sbjct: 507 VWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534

BLAST of Spg035936 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 466.1 bits (1198), Expect = 4.4e-131
Identity = 235/458 (51.31%), Postives = 322/458 (70.31%), Query Frame = 0

Query: 145 IITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLS-SLPFIVIDLINLTTISIDH 204
           IITP+Q + +Q  + C++ + + IR RSGGHDYEGLSY++ S PF+VIDL NL +I++D 
Sbjct: 85  IITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDV 144

Query: 205 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 264
            N T WVQSGAT+GELYY+I K S +LAFPAG+ PTVG+GG F GGG GTL RKYGL+AD
Sbjct: 145 DNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSAD 204

Query: 265 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 324
           NVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V++WK++L+ VP +VT   +  
Sbjct: 205 NVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVK 264

Query: 325 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTLGAKTNPTASFTSLFLGKANELMAIL 384
           T E++AV +I++WQY+A+KV  +LF+  +L       +T   ASF  L+LG  ++L+A++
Sbjct: 265 TSEKEAVSIINKWQYIADKVPNDLFIRAMLQ-----KETEVYASFPGLYLGPVSDLLALM 324

Query: 385 NTTFPELSLAKKDCIESSWINSTLTIAGFTPGEALETLLNRKSPTTKSTKVKSDYVKEPI 444
              FPEL L   +C E SWI S L    F  GE++E L  RK  T++S K K D+++EPI
Sbjct: 325 KDKFPELGLEIGNCREMSWIESVL---WFIKGESMEILAKRKR-TSRSFKGKDDFIEEPI 384

Query: 445 SEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIKWQEE 504
            + AI+ LW+R +A +  + K++  P+GG+MS+I ++E P+ HR+G LY I Y   W EE
Sbjct: 385 PKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEE 444

Query: 505 SIDAEK----RQVNWMRKLFDYMTPFVSKLPRASYSNYRDLD----LGTNNKYGATSYKQ 564
             D  K    + + W+  ++++MTP+VSK PR +Y N+RD+D    LG N K   T Y++
Sbjct: 445 E-DKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMK---TKYEE 504

Query: 565 ASVWGLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPIL 594
           A VWG+ YF NNFDRLV VKT VDP +FF  EQSIPI+
Sbjct: 505 AKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529

BLAST of Spg035936 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 463.4 bits (1191), Expect = 2.8e-130
Identity = 232/458 (50.66%), Postives = 319/458 (69.65%), Query Frame = 0

Query: 145 IITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLS-SLPFIVIDLINLTTISIDH 204
           IITP++ S +Q  + C++ + + +R RS GH YEGLSY++ + PF VIDL NL +IS+D 
Sbjct: 81  IITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDV 140

Query: 205 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 264
            N T WVQ+GAT GELYY+I KT+ +LAFPAG+ PTVGVGG FSGGG GTL RKYGLAAD
Sbjct: 141 DNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAAD 200

Query: 265 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 324
           N+IDA +VDA+G+  DR++MGED FWAIRGGGG SFG++++WKVKLV VP  +T   +  
Sbjct: 201 NIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQK 260

Query: 325 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTLGAKTNPTASFTSLFLGKANELMAIL 384
           T +++AV++I +WQY A+KV ++LF+   L       K    A FT L++G  N L+A++
Sbjct: 261 TSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN---KNAVHALFTGLYIGPVNNLLALM 320

Query: 385 NTTFPELSLAKKDCIESSWINSTLTIAGFTPGEALETLLNRKSPTTKSTKVKSDYVKEPI 444
              FPEL L K+ C E SWI S L  A F  GE+L  L NR+  T+ S K K D+V+EPI
Sbjct: 321 EEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLTNRER-TSLSFKGKDDFVQEPI 380

Query: 445 SEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIKWQEE 504
            EAAI+ +W+RL+A +  + K++  P+GG+MS++ E ETP+ HR G LY I Y   W+EE
Sbjct: 381 PEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREE 440

Query: 505 SIDAEK----RQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASVW 564
             D  K    + + W+  ++++MTP+VSK PR +Y N++D+DLG       T Y++   W
Sbjct: 441 E-DKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKKTKYEEGKSW 500

Query: 565 GLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIPILSSNT 598
           G+ YF NNF+RLV VKT+VDP +FF  EQSIP+++  T
Sbjct: 501 GVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVNKVT 533

BLAST of Spg035936 vs. TAIR 10
Match: AT4G20860.1 (FAD-binding Berberine family protein )

HSP 1 Score: 455.7 bits (1171), Expect = 5.9e-128
Identity = 226/451 (50.11%), Postives = 308/451 (68.29%), Query Frame = 0

Query: 144 VIITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSLPFIVIDLINLTTISIDH 203
           +I+ P   S I+ +++CS+K  +Q+R  SGGHDYEGLSYLS  PFI++DL+NL +ISI+ 
Sbjct: 83  LIVKPKSESEIKQSILCSRKLGVQVRTMSGGHDYEGLSYLSLSPFIIVDLVNLRSISINL 142

Query: 204 KNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGLAAD 263
            + TAW+QSGAT+GE+YYKI KTS   AF AG+CP+VGVGG  SGGG GT+ RKYGLA+D
Sbjct: 143 TDETAWIQSGATLGEVYYKIAKTSKIHAFAAGICPSVGVGGHISGGGFGTIMRKYGLASD 202

Query: 264 NVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCLLNV 323
           NV+DA L+D NGK  DR++MGEDLFWA+RGGG  SFG+V++WKVKL RVP  VT  +   
Sbjct: 203 NVVDARLMDVNGKTLDRKTMGEDLFWALRGGGAASFGVVLSWKVKLARVPEKVTCFISQH 262

Query: 324 TLEEDAVKLIHRWQYVANKVDENLFLAIVLTGGTLGAKTNPTASFTSLFLGKANELMAIL 383
            +     KL+HRWQ + +++DE+LF+ +++     G +    ++F +LFLG  + L+ ++
Sbjct: 263 PMGPSMNKLVHRWQSIGSELDEDLFIRVIIDNSLEGNQRKVKSTFQTLFLGGIDRLIPLM 322

Query: 384 NTTFPELSLAKKDCIESSWINSTLTIAGFTPGEALETLLNRKSP-TTKSTKVKSDYVKEP 443
           N  FPEL L  +DC E SWI S +    +  G+ LE LLNR      +  K KSDYV++P
Sbjct: 323 NQKFPELGLRSQDCSEMSWIES-IMFFNWRSGQPLEILLNRDLRFEDQYFKAKSDYVQKP 382

Query: 444 ISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYKIKWQE 503
           + E   + + KR   +D  +  ++F P GG++S+I E+E+PY HR+G LY I Y +KW+ 
Sbjct: 383 VPENVFEEVTKRFLEQDTPL--MIFEPLGGKISKISETESPYPHRRGNLYNIQYMVKWKV 442

Query: 504 ESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQASVWGLN 563
             ++   + V WMR L DYMTP+VSK PR +Y NYRDLDLG+      TS++ A  WG  
Sbjct: 443 NEVEEMNKHVRWMRSLHDYMTPYVSKSPRGAYLNYRDLDLGSTKGIN-TSFEDARKWGET 502

Query: 564 YFGNNFDRLVHVKTKVDPYNFFMHEQSIPIL 594
           YF  NF RL  VK K+DP NFF +EQSIP L
Sbjct: 503 YFKGNFKRLGLVKGKIDPTNFFRNEQSIPPL 529

BLAST of Spg035936 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 451.4 bits (1160), Expect = 1.1e-126
Identity = 223/454 (49.12%), Postives = 312/454 (68.72%), Query Frame = 0

Query: 143 EVIITPLQVSHIQVAVICSKKNALQIRIRSGGHDYEGLSYLSSL--PFIVIDLINLTTIS 202
           E I  PL  +H+Q AV+C+KK  L +R+RSGGHDYEGLSY+S +   F+++DL  L  IS
Sbjct: 85  EFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVDLSKLRQIS 144

Query: 203 IDHKNNTAWVQSGATVGELYYKIFKTSPTLAFPAGVCPTVGVGGLFSGGGSGTLQRKYGL 262
           +D ++N+AWV +GA++GE+YY+I + S    FPAG+C ++G+GG   GG  G++ RK+GL
Sbjct: 145 VDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMMRKFGL 204

Query: 263 AADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPRIVTSCL 322
            ADNV+DA +VDA+GK  +R +MGED+FWAIRGGGGGSFG+++AWK+KLV VP IVT   
Sbjct: 205 GADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEIVTVFT 264

Query: 323 LNVTLEEDAVKLIHRWQYVANKVDENLFLAIVL--TGGTLGAKTNP-TASFTSLFLGKAN 382
           +  TLE+D  KL+++WQ VA+K+DE+LF+ +++  T  T  +K    + S+   FLG AN
Sbjct: 265 VTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTISTSYQGQFLGDAN 324

Query: 383 ELMAILNTTFPELSLAKKDCIESSWINSTLTIAGFTPGEALETLLNRKSPTTKSTKVKSD 442
            L+ ++  +FP+L L KKDC+E+SWI S + IAGF      E LL+ KS      K KSD
Sbjct: 325 RLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKSLFKNYFKAKSD 384

Query: 443 YVKEPISEAAIKGLWKRLKARDIEVIKLLFIPYGGRMSQIFESETPYSHRKGYLYRIGYK 502
           YV+EPI    ++GLW++L   D  +   ++ PYGG M++I E+ETP+ HR G L++I + 
Sbjct: 385 YVEEPIPVEGLEGLWEKLLEEDSPL--TIWNPYGGMMAKIPETETPFPHRSGTLFKIQWL 444

Query: 503 IKWQEESIDAEKRQVNWMRKLFDYMTPFVSKLPRASYSNYRDLDLGTNNKYGATSYKQAS 562
             WQ+    +E + + WMR+++ YM  +VSK PR++Y NYRDLDLG N K        A 
Sbjct: 445 TLWQDGK-TSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGK-----GSDAR 504

Query: 563 VWGLNYFGNNFDRLVHVKTKVDPYNFFMHEQSIP 592
            WG  YF  NF+RLV +K K DP NFF HEQSIP
Sbjct: 505 EWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530