Spg035891 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg035891 vs. NCBI nr
Match: XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 792.7 bits (2046), Expect = 1.8e-225
Identity = 398/538 (73.98%), Postives = 443/538 (82.34%), Query Frame = 0

Query: 3   SSPLVPL--SLAFILLSSLS----------------------SVNSSISKVVYTQINSSY 62
           SSPL+PL  SL FI LSS S                        N SISKVVYT +NSSY
Sbjct: 4   SSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSY 63

Query: 63  SSVLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFEGLS 122
           SSVL+FSIRNLRFSTA+TPKPL IITP HVSH+QAAV+CS+TH LQIRTRSGGHDFEGLS
Sbjct: 64  SSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLS 123

Query: 123 YVAHLPFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGVCPTVG 182
            V+HLPFII+DLINL SIT+D  NNTAWVQSGATLGELYY IAEK+RTLAF AG CPTVG
Sbjct: 124 CVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVG 183

Query: 183 VGGHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGGGSFGI 242
           +GGHFSGGGYGLMLRK+GLAAD+VIDAYLVDA+GKF DRE MGEDLFWA+RGGGGGSFGI
Sbjct: 184 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGI 243

Query: 243 VVAWKVKLVPVPATVTICSTGRNLEEDVIELIYRWQHVANKLDENLFLGIILTGG----- 302
           VVAWKVKLVPVPATVT C   R LEE  I+LI+RWQ+VANKLDENLFLGI+L GG     
Sbjct: 244 VVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQ 303

Query: 303 ---KTKPTATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESALI-LGIEIGQP 362
              KT PTA+FFSLFLGK DE   I  TTFP LGLAK++C+E+SWIESAL+  G +IG  
Sbjct: 304 GGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSAGFKIGDS 363

Query: 363 LEALLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGRMDQI 422
           LEALLNRTP T+G  KIKSDYVKEPIS A I+GIWERLKS D+   QL+FVPYGGRM QI
Sbjct: 364 LEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGGRMSQI 423

Query: 423 SESEIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWIRELYNYMAPFVSKLPRGAYANY 482
           SESEIPF HRAGNLYKI Y V W++ ++DV+K+HL+WIRELY+YM PFVSK PR AY NY
Sbjct: 424 SESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYVNY 483

Query: 483 RDLDIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIPALSP 508
           RDLDIGSNNKYGKT ++RASVWG+KYFG NFNRLV+VKTKVDP DFF+HEQSIP +SP
Sbjct: 484 RDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTISP 541

BLAST of Spg035891 vs. NCBI nr
Match: KAG6583829.1 (Berberine bridge enzyme-like 26, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 779.2 bits (2011), Expect = 2.1e-221
Identity = 383/528 (72.54%), Postives = 437/528 (82.77%), Query Frame = 0

Query: 7   VPLSLAFILLSSLSSVNSS----------------------ISKVVYTQINSSYSSVLNF 66
           +PL+L FI++ S S + +S                      ISK+VYT INSSYS VLNF
Sbjct: 4   IPLTLIFIIVLSSSPLWASPSLHIHQHFLHCLSHHSPHTYPISKLVYTPINSSYSYVLNF 63

Query: 67  SIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFEGLSYVAHLP 126
           SIRNL FS   TPKPL+IITPSH+SHIQAAVICSK H+LQIRTRSGGHDFEGLSYVA  P
Sbjct: 64  SIRNLIFSNPQTPKPLLIITPSHISHIQAAVICSKAHDLQIRTRSGGHDFEGLSYVADQP 123

Query: 127 FIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGVCPTVGVGGHFS 186
           FIIVDLINL SI VDV+ NTAWVQSGATLGELYYRI EK+R+LAF AG+CPTVGVGGHFS
Sbjct: 124 FIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGEKSRSLAFPAGICPTVGVGGHFS 183

Query: 187 GGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGGGSFGIVVAWKV 246
           GGGYGLMLRKYGLAAD+V+DAYLVDA+G+ HDRESMGEDLFWA+RGGGGGSFGIVVAWKV
Sbjct: 184 GGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 243

Query: 247 KLVPVPATVTICSTGRNLEEDVIELIYRWQHVANKLDENLFLGIILTGGK-------TKP 306
           KLV VPA VT+CST R++E+D I+LI+RWQ+VAN+LD++LFLGI+LTGG        T P
Sbjct: 244 KLVVVPAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDDLFLGILLTGGNFSTREGITNP 303

Query: 307 TATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESALI--LGIEIGQPLEALLN 366
           TATFFSL+LGK+DEL+ I KTTFP LGL KKDC+E+SWI+S LI   G++  +PLEALLN
Sbjct: 304 TATFFSLYLGKLDELIAILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLN 363

Query: 367 RTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGRMDQISESEIP 426
           RTPPT   TK KSDYVK+PI  AAI+GIW+RLK+ DIEVPQ++F+PYGGRM QISESE P
Sbjct: 364 RTPPTIESTKFKSDYVKDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETP 423

Query: 427 FPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWIRELYNYMAPFVSKLPRGAYANYRDLDIG 486
           F HRAGNLYKI YAV WKE S+D   +H+ WIRE+Y YMAPFVSK PR AY NYRDLDIG
Sbjct: 424 FSHRAGNLYKIGYAVSWKEQSLDAKNRHINWIREVYEYMAPFVSKSPRAAYVNYRDLDIG 483

Query: 487 SNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIP 504
           +NNKYGKT Y +ASVWG KYFG NFNRLVYVKT VDP+DFF+HEQSIP
Sbjct: 484 TNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTMVDPHDFFRHEQSIP 531

BLAST of Spg035891 vs. NCBI nr
Match: XP_038895543.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 778.9 bits (2010), Expect = 2.7e-221
Identity = 389/533 (72.98%), Postives = 439/533 (82.36%), Query Frame = 0

Query: 3   SSPLVPLSLAFIL---------------------LSSLSSVNSSISKVVYTQINSSYSSV 62
           SSPL+PL+L  I+                     LS  SS +  ISKVVY+ INSSYSSV
Sbjct: 4   SSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSYSSV 63

Query: 63  LNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFEGLSYVA 122
           L+FSI+NLRFS   TPKPL+IITPSHVSHIQAAVICSK+H LQIRTRSGGHDFEGLSYVA
Sbjct: 64  LDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVA 123

Query: 123 HLPFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGVCPTVGVGG 182
           + PFI+VDLINL SI+VD ENNTAWV+SGATLGELYYRI EK+RTLAF AGVCPTVG+GG
Sbjct: 124 YRPFIVVDLINLRSISVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGG 183

Query: 183 HFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGGGSFGIVVA 242
           HFSGGGYGLMLRK+GLAAD+VIDA+LVDA+GK HDRESMGEDLFWA+RGGGGGSFGIVVA
Sbjct: 184 HFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVA 243

Query: 243 WKVKLVPVPATVTICSTGRNLEEDVIELIYRWQHVANKLDENLFLGIILTGGK------- 302
           WK+KLVPVPATVTICST RNLEED I+L++RWQ+VANKLDENL+LGIILTGGK       
Sbjct: 244 WKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKASTQGGI 303

Query: 303 TKPTATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESALIL--GIEIGQPLEA 362
           T PTATFFSLFLG+IDEL+    TTFP LGL K+DC+E +W+ES LI+  G++  + LE 
Sbjct: 304 TNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIESLEP 363

Query: 363 LLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGRMDQISES 422
           LLNRTPPT   TKIKSDYVKEP+  AAI+GI +RLK+ DIE  QLIFVPYGG+M QISES
Sbjct: 364 LLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQISES 423

Query: 423 EIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWIRELYNYMAPFVSKLPRGAYANYRDL 482
           E PF HRAGNLYKI Y VGWKE S+   K+H+ WIRE+Y YM PFVSK PR AYANYRDL
Sbjct: 424 ETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANYRDL 483

Query: 483 DIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIPAL 506
           DIG+NNKYG+T   +ASVWG KYFG NF +LVYVKTKVDP DFF+HEQSIP L
Sbjct: 484 DIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Spg035891 vs. NCBI nr
Match: XP_023520318.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 778.9 bits (2010), Expect = 2.7e-221
Identity = 383/528 (72.54%), Postives = 437/528 (82.77%), Query Frame = 0

Query: 7   VPLSLAFILLSSLSSVNSS----------------------ISKVVYTQINSSYSSVLNF 66
           +PL+L FI++ S S + +S                      ISK+VYT INSSYSSVLNF
Sbjct: 4   IPLTLIFIIVLSSSPLWASPSLHIHQHFLHCLSHHSPHTYPISKLVYTPINSSYSSVLNF 63

Query: 67  SIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFEGLSYVAHLP 126
           SIRNL FS   TPKPL+IITPSH+SHIQAAVICSK H+LQIRTRSGGHDFEGLSYVA  P
Sbjct: 64  SIRNLIFSNPQTPKPLLIITPSHISHIQAAVICSKAHDLQIRTRSGGHDFEGLSYVADQP 123

Query: 127 FIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGVCPTVGVGGHFS 186
           FIIVDLINL SI VDV+ NTAWVQSGATLGELYYRI EK+R+LAF AG+CPTVGVGGHFS
Sbjct: 124 FIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGEKSRSLAFPAGICPTVGVGGHFS 183

Query: 187 GGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGGGSFGIVVAWKV 246
           GGGYGLMLRKYGLAAD+V+DAYLVDA+G+ HDRESMGEDLFWA+RGGGGGSFGIVVAWKV
Sbjct: 184 GGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 243

Query: 247 KLVPVPATVTICSTGRNLEEDVIELIYRWQHVANKLDENLFLGIILTGGK-------TKP 306
           KLV VPA VT+CST R++E+D I+LI+RWQ+VAN+LD+NLFLGI+LTGG        T P
Sbjct: 244 KLVVVPAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTRQGITNP 303

Query: 307 TATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESALI--LGIEIGQPLEALLN 366
           TATFFSL+LGK+DEL+ I KTTFP LGL KKDC+E+SWI+S LI   G++  +PLEALLN
Sbjct: 304 TATFFSLYLGKLDELIAILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLN 363

Query: 367 RTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGRMDQISESEIP 426
           RTPPT   TK KSDYVK+PI  AAI+GIW+RLK+ DIEVPQ++F+PYGGRM QISESE P
Sbjct: 364 RTPPTIESTKFKSDYVKDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETP 423

Query: 427 FPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWIRELYNYMAPFVSKLPRGAYANYRDLDIG 486
           F HRAGNLYKI YAV WKE S+D   +H+ WIRE+  +M PFVSK PR AY NYRDLDIG
Sbjct: 424 FSHRAGNLYKIGYAVSWKEQSLDAKNRHINWIREVNEHMTPFVSKSPRAAYVNYRDLDIG 483

Query: 487 SNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIP 504
           +NNKYGKT Y +ASVWG KYFG NFNRLVYVKTKVDP+DFF+HEQSIP
Sbjct: 484 TNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

BLAST of Spg035891 vs. NCBI nr
Match: XP_022152518.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 776.9 bits (2005), Expect = 1.0e-220
Identity = 388/537 (72.25%), Postives = 439/537 (81.75%), Query Frame = 0

Query: 1   MKSSPLVPLSLAFILLSSLSS---------------------VNSSISKVVYTQINSSYS 60
           MK SPL  LSLAFILLSS SS                      +SSISKV+YT IN SYS
Sbjct: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60

Query: 61  SVLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFEGLSY 120
           SVLNFS+RNLRFS+A TPKPL+IITPS+VSH+QAAVICSKTH LQIR RSGGHD EGLSY
Sbjct: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120

Query: 121 VAHLPFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGVCPTVGV 180
           VAHLPFII+DLINL SITV++EN+TAW+QSGATLGELYY IA+K+RTLAF AGVCPTVGV
Sbjct: 121 VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV 180

Query: 181 GGHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGGGSFGIV 240
           GGHFSGGGYGLMLRKYGLAAD+VIDAYL+DA+GKF+DRESMGEDLFWA+RGGGGGSFGIV
Sbjct: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240

Query: 241 VAWKVKLVPVPATVTICSTGRNLEEDVIELIYRWQHVANKLDENLFLGIILTGG------ 300
           VAWKVKLVPVPA VT CS+ RNL+ED I LI+RWQ VA KLDENL+ GIIL GG      
Sbjct: 241 VAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEG 300

Query: 301 ---KTKPTATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESALILGI--EIGQ 360
              K  PTATFFSLFLGK DEL+ I  T FP L LAKKDC+ETSWIES L+     +  +
Sbjct: 301 GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE 360

Query: 361 PLEALLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGRMDQ 420
            LE+LLNRTP T   TK+KSDYVKEPI  A I+GIWERLK+ DIEVPQL+FVPYGGRM +
Sbjct: 361 SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR 420

Query: 421 ISESEIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWIRELYNYMAPFVSKLPRGAYAN 480
           IS+SE PF HRA NLYKI Y VGWKE S+  +++HL WIRE+Y+YM PFVSK PR  Y N
Sbjct: 421 ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN 480

Query: 481 YRDLDIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIPAL 506
           YRDLDIG+NNKYGKT Y++ASVWG KYFG NFNRLV++K+KVDP++FF++EQSIP L
Sbjct: 481 YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537

BLAST of Spg035891 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 9.4e-148
Identity = 261/503 (51.89%), Postives = 347/503 (68.99%), Query Frame = 0

Query: 23  NSSISKVVYTQINSSYSSVLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHN 82
           N +  K+VYTQ +  Y S+LN +I+NLRF +  TPKPLVI+TPS+ SHIQA ++CSK   
Sbjct: 45  NVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVG 104

Query: 83  LQIRTRSGGHDFEGLSYVAHLPFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAE 142
           LQIRTRSGGHD EG+SY++ +PF++VDL N+ SI +DV + TAWV++GATLGE+YY I E
Sbjct: 105 LQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINE 164

Query: 143 KNRTLAFLAGVCPTVGVGGHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGE 202
           KN  L+F  G CPTVGVGGHFSGGGYG ++R YGLAAD++IDA+LV+ DGK  DR+SMGE
Sbjct: 165 KNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGE 224

Query: 203 DLFWAVRGGGGGSFGIVVAWKVKLVPVPATVTICSTGRNLE-EDVIELIYRWQHVANKLD 262
           DLFWA+RGGGG +FGI+ AWK+KLV VP+  TI S  +N+E   +++L  +WQ++A K D
Sbjct: 225 DLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYD 284

Query: 263 ENLFL-------GIILTGGKTKPT--ATFFSLFLGKIDELVTIFKTTFPALGLAKKDCME 322
           ++L L        I    GK K T    F S+F G +D LV +   +FP LG+ K DC E
Sbjct: 285 KDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKE 344

Query: 323 TSWIESALILGIEI-----GQPLEALLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERL 382
            SWI++ +     +         E LL+R+        IK DYVK+PI   A+  I E+L
Sbjct: 345 FSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKL 404

Query: 383 KSHDIEVPQLIFVPYGGRMDQISESEIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWI 442
              D+     +  PYGG M++ISES IPFPHRAG +Y++ Y   W++   + ++KH+ W+
Sbjct: 405 YEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWV 464

Query: 443 RELYNYMAPFVSKLPRGAYANYRDLDIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVK 502
           R +YN+  P+VS+ PR AY NYRDLD+G  N      Y +A +WG KYFGKNFNRLV VK
Sbjct: 465 RSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVK 524

Query: 503 TKVDPNDFFQHEQSIPALSPTYN 511
           TKVDPN+FF++EQSIP L P ++
Sbjct: 525 TKVDPNNFFRNEQSIPPLPPHHH 545

BLAST of Spg035891 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 2.1e-147
Identity = 259/498 (52.01%), Postives = 347/498 (69.68%), Query Frame = 0

Query: 23  NSSISKVVYTQINSSYSSVLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHN 82
           N + +K+VYTQ +  Y S+LN +I+NLRF++  TPKPLVIITP +VSHIQ  ++CSK   
Sbjct: 45  NVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVSHIQGTILCSKKVG 104

Query: 83  LQIRTRSGGHDFEGLSYVAHLPFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAE 142
           LQIRTRSGGHD EG+SY++ +PF+IVDL N+ S+ +DV + TAWV++GATLGE+YY I E
Sbjct: 105 LQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEAGATLGEVYYWINE 164

Query: 143 KNRTLAFLAGVCPTVGVGGHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGE 202
            N  L+F AG CPTVG GGHFSGGGYG ++R YGLAAD++IDA+LV+ DGK  DR+SMGE
Sbjct: 165 NNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGE 224

Query: 203 DLFWAVRGGGGGSFGIVVAWKVKLVPVPATVTICSTGRNLE-EDVIELIYRWQHVANKLD 262
           DLFWA+RGGGG +FGI+ AWK++LV VP+  TI S  +N+E  ++++L+ +WQ++A   +
Sbjct: 225 DLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYE 284

Query: 263 ENLFL-------GIILTGGKTKPT--ATFFSLFLGKIDELVTIFKTTFPALGLAKKDCME 322
           + L L        I    GK K T  + F S+F G +D LV +   +FP LG+ K DC +
Sbjct: 285 KELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQ 344

Query: 323 TSWIESALILGIEIGQ-----PLEALLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERL 382
            SWI++ +     +         E LL+R+        IK DYVK+PI   A+  I E+L
Sbjct: 345 LSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKL 404

Query: 383 KSHDIEVPQLIFVPYGGRMDQISESEIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWI 442
              D+ V   +F PYGG MD+ISES IPFPHRAG +Y+I Y   W++   + ++KH+ WI
Sbjct: 405 YEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEKQ--EDNEKHINWI 464

Query: 443 RELYNYMAPFVSKLPRGAYANYRDLDIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVK 502
           R +YN+  P+VS+ PR AY NYRDLD+G  N      Y +A +WG KYFGKNFNRLV VK
Sbjct: 465 RNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVK 524

Query: 503 TKVDPNDFFQHEQSIPAL 506
           TKVDP++FF++EQSIP L
Sbjct: 525 TKVDPDNFFRNEQSIPPL 540

BLAST of Spg035891 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 3.6e-147
Identity = 260/503 (51.69%), Postives = 346/503 (68.79%), Query Frame = 0

Query: 23  NSSISKVVYTQINSSYSSVLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHN 82
           N +  K +YTQ +  Y SVLN +I+NLRF++  TPKPLVI+TPS+VSHIQA+++CSK   
Sbjct: 45  NPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVG 104

Query: 83  LQIRTRSGGHDFEGLSYVAHLPFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAE 142
           LQIRTRSGGHD EGLSY++ +PF IVDL N+ ++ VD+ + TAWV++GATLGE+YY I E
Sbjct: 105 LQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINE 164

Query: 143 KNRTLAFLAGVCPTVGVGGHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGE 202
            N   +F  G CPTVGVGGHFSGGGYG ++R YGLAAD++IDA+LV+ DGK  DR+SMGE
Sbjct: 165 MNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGE 224

Query: 203 DLFWAVRGGGGGSFGIVVAWKVKLVPVPATVTICSTGRNLE-EDVIELIYRWQHVANKLD 262
           DLFWA+RGGGG +FGI+ AWK+KLV VP+  TI S  +N+E   +++L  +WQ++A K D
Sbjct: 225 DLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYD 284

Query: 263 ENLFL-------GIILTGGKTKPT--ATFFSLFLGKIDELVTIFKTTFPALGLAKKDCME 322
           ++L L        I    GK K T    F S+FLG +D LV +   +FP LG+ K DC E
Sbjct: 285 KDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKE 344

Query: 323 TSWIESALILGIEIGQ-----PLEALLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERL 382
            SWI++ +     +         E LL+R+        IK DYVK+ I   A+  I E+L
Sbjct: 345 LSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKL 404

Query: 383 KSHDIEVPQLIFVPYGGRMDQISESEIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWI 442
              ++ V   +  PYGG MD+ISES IPFPHRAG +Y++ Y   W++   + ++KH+ W+
Sbjct: 405 YEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATWEKQ--EDNEKHINWV 464

Query: 443 RELYNYMAPFVSKLPRGAYANYRDLDIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVK 502
           R +YN+  P+VS+ PR AY NYRDLD+G  N      Y +A +WG KYFGKNFNRLV VK
Sbjct: 465 RSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVK 524

Query: 503 TKVDPNDFFQHEQSIPALSPTYN 511
           TK DPN+FF++EQSIP L P ++
Sbjct: 525 TKADPNNFFRNEQSIPPLPPHHH 545

BLAST of Spg035891 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.4e-146
Identity = 260/505 (51.49%), Postives = 347/505 (68.71%), Query Frame = 0

Query: 16  LSSLSSVNSSISKVVYTQINSSYSSVLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAV 75
           LS     N + +K+VYTQ +  Y S+LN +++NLRF++  TPKPLVI TP +VSHIQ  +
Sbjct: 38  LSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVSHIQGTI 97

Query: 76  ICSKTHNLQIRTRSGGHDFEGLSYVAHLPFIIVDLINLSSITVDVENNTAWVQSGATLGE 135
           +CSK   LQIRTRSGGHD EG+SY++ +PF+IVDL N+ S+ +DV + TAWV+SGATLGE
Sbjct: 98  LCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVESGATLGE 157

Query: 136 LYYRIAEKNRTLAFLAGVCPTVGVGGHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFH 195
           +YY I E N  L+F AG CPTVG GGHFSGGGYG ++R YGLAAD++IDA+LV+ DGK  
Sbjct: 158 VYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVL 217

Query: 196 DRESMGEDLFWAVRGGGGGSFGIVVAWKVKLVPVPATVTICSTGRNLE-EDVIELIYRWQ 255
           DR+SMGEDLFWA+RGGGG +FGI+ AWK++LV VP+  TI S  +N+E  ++++L+ +WQ
Sbjct: 218 DRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQ 277

Query: 256 HVANKLDENLFL-------GIILTGGKTKPT--ATFFSLFLGKIDELVTIFKTTFPALGL 315
           ++A   ++ L L        I    GK K T  + F S+F G +D LV +   +FP LG+
Sbjct: 278 NIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGI 337

Query: 316 AKKDCMETSWIESALILGIEIGQ-----PLEALLNRTPPTSGRTKIKSDYVKEPISMAAI 375
            K DC + SWI++ +     +         E LL+R+        IK DYVK+PI   A+
Sbjct: 338 KKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKPIPETAM 397

Query: 376 KGIWERLKSHDIEVPQLIFVPYGGRMDQISESEIPFPHRAGNLYKIAYAVGWKEGSIDVD 435
             I E+L   D+ V   +F PYGG MD+ISES IPFPHRAG  Y+I Y   W++   + +
Sbjct: 398 VTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEKQ--EDN 457

Query: 436 KKHLRWIRELYNYMAPFVSKLPRGAYANYRDLDIGSNNKYGKTGYRRASVWGFKYFGKNF 495
           +KH+ WIR +YN+  P+VS+ PR AY NYRDLD+G  N      Y +A +WG KYFGKNF
Sbjct: 458 EKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEKYFGKNF 517

Query: 496 NRLVYVKTKVDPNDFFQHEQSIPAL 506
           NRLV VKTKVDP++FF++EQSIP L
Sbjct: 518 NRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Spg035891 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 8.8e-146
Identity = 263/498 (52.81%), Postives = 344/498 (69.08%), Query Frame = 0

Query: 23  NSSISKVVYTQINSSYSSVLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHN 82
           N++  K+VYTQ N  Y SVLN +I NLRF++  TPKPLVI+TPSHVSHIQ  ++CSK   
Sbjct: 45  NATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVG 104

Query: 83  LQIRTRSGGHDFEGLSYVAHLPFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAE 142
           LQIRTRSGGHD EG+SY++ +PF+IVDL N+ SI +DV + TAWV++GATLGE+YY + E
Sbjct: 105 LQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNE 164

Query: 143 KNRTLAFLAGVCPTVGVGGHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGE 202
           KN  L+  AG CPTV  GGHF GGGYG ++R YGLAAD++IDA+LV+  GK  DR+SMGE
Sbjct: 165 KNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGE 224

Query: 203 DLFWAVRGGGGGSFGIVVAWKVKLVPVPATVTICSTGRNLE-EDVIELIYRWQHVANKLD 262
           DLFWA+RGGG  SFGI+VAWK++LV VP + T+ S  + +E  ++++L+ +WQ++A K D
Sbjct: 225 DLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKKIMEIHELVKLVNKWQNIAYKYD 284

Query: 263 ENLFL-------GIILTGGKTKPT--ATFFSLFLGKIDELVTIFKTTFPALGLAKKDCME 322
           ++L L        I    GK K      F S+FLG +D LV +   +FP LG+ K DC +
Sbjct: 285 KDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQ 344

Query: 323 TSWIESALILGIEIGQPL-----EALLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERL 382
            SWI++ +     +         E LL+R+   +G  KIK DYVK+PI  +    I E+L
Sbjct: 345 LSWIDTIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKL 404

Query: 383 KSHDIEVPQLIFVPYGGRMDQISESEIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWI 442
              DI        PYGG MD+ISES IPFPHRAG LY++ Y   W++   + ++KHL WI
Sbjct: 405 YEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWI 464

Query: 443 RELYNYMAPFVSKLPRGAYANYRDLDIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVK 502
           R +YN+M P+VSK PR AY NYRDLDIG N+      Y +A +WG KYFGKNF+RLV VK
Sbjct: 465 RNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVK 524

Query: 503 TKVDPNDFFQHEQSIPAL 506
           T VDPN+FF++EQSIP L
Sbjct: 525 TLVDPNNFFRNEQSIPPL 539

BLAST of Spg035891 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 8.9e-226
Identity = 398/538 (73.98%), Postives = 443/538 (82.34%), Query Frame = 0

Query: 3   SSPLVPL--SLAFILLSSLS----------------------SVNSSISKVVYTQINSSY 62
           SSPL+PL  SL FI LSS S                        N SISKVVYT +NSSY
Sbjct: 4   SSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSY 63

Query: 63  SSVLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFEGLS 122
           SSVL+FSIRNLRFSTA+TPKPL IITP HVSH+QAAV+CS+TH LQIRTRSGGHDFEGLS
Sbjct: 64  SSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLS 123

Query: 123 YVAHLPFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGVCPTVG 182
            V+HLPFII+DLINL SIT+D  NNTAWVQSGATLGELYY IAEK+RTLAF AG CPTVG
Sbjct: 124 CVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVG 183

Query: 183 VGGHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGGGSFGI 242
           +GGHFSGGGYGLMLRK+GLAAD+VIDAYLVDA+GKF DRE MGEDLFWA+RGGGGGSFGI
Sbjct: 184 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGI 243

Query: 243 VVAWKVKLVPVPATVTICSTGRNLEEDVIELIYRWQHVANKLDENLFLGIILTGG----- 302
           VVAWKVKLVPVPATVT C   R LEE  I+LI+RWQ+VANKLDENLFLGI+L GG     
Sbjct: 244 VVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQ 303

Query: 303 ---KTKPTATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESALI-LGIEIGQP 362
              KT PTA+FFSLFLGK DE   I  TTFP LGLAK++C+E+SWIESAL+  G +IG  
Sbjct: 304 GGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSAGFKIGDS 363

Query: 363 LEALLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGRMDQI 422
           LEALLNRTP T+G  KIKSDYVKEPIS A I+GIWERLKS D+   QL+FVPYGGRM QI
Sbjct: 364 LEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGGRMSQI 423

Query: 423 SESEIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWIRELYNYMAPFVSKLPRGAYANY 482
           SESEIPF HRAGNLYKI Y V W++ ++DV+K+HL+WIRELY+YM PFVSK PR AY NY
Sbjct: 424 SESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYVNY 483

Query: 483 RDLDIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIPALSP 508
           RDLDIGSNNKYGKT ++RASVWG+KYFG NFNRLV+VKTKVDP DFF+HEQSIP +SP
Sbjct: 484 RDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTISP 541

BLAST of Spg035891 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 5.1e-221
Identity = 388/537 (72.25%), Postives = 439/537 (81.75%), Query Frame = 0

Query: 1   MKSSPLVPLSLAFILLSSLSS---------------------VNSSISKVVYTQINSSYS 60
           MK SPL  LSLAFILLSS SS                      +SSISKV+YT IN SYS
Sbjct: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60

Query: 61  SVLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFEGLSY 120
           SVLNFS+RNLRFS+A TPKPL+IITPS+VSH+QAAVICSKTH LQIR RSGGHD EGLSY
Sbjct: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120

Query: 121 VAHLPFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGVCPTVGV 180
           VAHLPFII+DLINL SITV++EN+TAW+QSGATLGELYY IA+K+RTLAF AGVCPTVGV
Sbjct: 121 VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV 180

Query: 181 GGHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGGGSFGIV 240
           GGHFSGGGYGLMLRKYGLAAD+VIDAYL+DA+GKF+DRESMGEDLFWA+RGGGGGSFGIV
Sbjct: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240

Query: 241 VAWKVKLVPVPATVTICSTGRNLEEDVIELIYRWQHVANKLDENLFLGIILTGG------ 300
           VAWKVKLVPVPA VT CS+ RNL+ED I LI+RWQ VA KLDENL+ GIIL GG      
Sbjct: 241 VAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEG 300

Query: 301 ---KTKPTATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESALILGI--EIGQ 360
              K  PTATFFSLFLGK DEL+ I  T FP L LAKKDC+ETSWIES L+     +  +
Sbjct: 301 GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE 360

Query: 361 PLEALLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGRMDQ 420
            LE+LLNRTP T   TK+KSDYVKEPI  A I+GIWERLK+ DIEVPQL+FVPYGGRM +
Sbjct: 361 SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR 420

Query: 421 ISESEIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWIRELYNYMAPFVSKLPRGAYAN 480
           IS+SE PF HRA NLYKI Y VGWKE S+  +++HL WIRE+Y+YM PFVSK PR  Y N
Sbjct: 421 ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN 480

Query: 481 YRDLDIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIPAL 506
           YRDLDIG+NNKYGKT Y++ASVWG KYFG NFNRLV++K+KVDP++FF++EQSIP L
Sbjct: 481 YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537

BLAST of Spg035891 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 5.1e-221
Identity = 389/537 (72.44%), Postives = 440/537 (81.94%), Query Frame = 0

Query: 4   SPLVPLSLAFILLS-----------------------SLSSVNSSISKVVYTQINSSYSS 63
           SPL+ LSLAFI LS                       SL   N SISKV++T INSSYSS
Sbjct: 5   SPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSS 64

Query: 64  VLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFEGLSYV 123
           VL+FSIRNLRFST  TPKPL+I+TPSHVSHIQAAVICSK++  QIRTRSGGHDFEGLSYV
Sbjct: 65  VLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYV 124

Query: 124 AHLPFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGVCPTVGVG 183
           AHLPFIIVDLINL SI++D+++NTAWVQSGATLGELYY IA+K+RTLAF AG+CPTVGVG
Sbjct: 125 AHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVG 184

Query: 184 GHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGGGSFGIVV 243
           GHFSGGGYGL+LRKYGLAAD+VIDAYLVDA GKFH+RESMGEDLFWA+RGGGGGSFGIVV
Sbjct: 185 GHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVV 244

Query: 244 AWKVKLVPVPATVTICSTGRNLEEDVIELIYRWQHVANKLDENLFLGII--------LTG 303
           AWKVKLVPVPATVT+CS  R LEE  +++ +RWQ+VANKLDENLFLGI+          G
Sbjct: 245 AWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEG 304

Query: 304 GKTKPTATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESALILGIEIGQP--- 363
           GKT P ATFFSLFLGK DEL++I  T FP L LAKKDC+ETSWIES +++G  +G P   
Sbjct: 305 GKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMG--VGFPTEL 364

Query: 364 -LEALLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGRMDQ 423
            LEALL+RTP T+  +KIKSDYVKEP+S A ++GIW+RL S DIE   +IFVPYGGRM Q
Sbjct: 365 TLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQ 424

Query: 424 ISESEIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWIRELYNYMAPFVSKLPRGAYAN 483
           ISESE PF HRAG LYKI Y  GW+E  ID +K+HLRWIRELY+YM PFVSK PR AYAN
Sbjct: 425 ISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYAN 484

Query: 484 YRDLDIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIPAL 506
           YRDLDIGSNNK+GKT Y++ASVWG KYFG NFNRLV+VKTKVDP DFF+HEQSIPAL
Sbjct: 485 YRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of Spg035891 vs. ExPASy TrEMBL
Match: A0A6J1KHF6 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111495284 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 8.6e-221
Identity = 380/528 (71.97%), Postives = 435/528 (82.39%), Query Frame = 0

Query: 7   VPLSLAFILLSSLSSVNSS----------------------ISKVVYTQINSSYSSVLNF 66
           +PL+L FI++ S S + +S                      ISK+VYT INSSYS VLNF
Sbjct: 4   IPLTLIFIIVLSSSPLWASPSLHKHQHFLHCLSHHSPHTYPISKLVYTPINSSYSPVLNF 63

Query: 67  SIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFEGLSYVAHLP 126
           SIRNL FS   TPKPL+IITPSH+SHIQAA++CSK H+LQIRTRSGGHDFEGLSYVA+ P
Sbjct: 64  SIRNLIFSNPQTPKPLLIITPSHISHIQAAIVCSKAHDLQIRTRSGGHDFEGLSYVANQP 123

Query: 127 FIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGVCPTVGVGGHFS 186
           FIIVDLINL SI VDV+ NTAWVQSGATLGELYYRI E +R+LAF AG+CPTVGVGGHFS
Sbjct: 124 FIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGETSRSLAFPAGICPTVGVGGHFS 183

Query: 187 GGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGGGSFGIVVAWKV 246
           GGGYGLMLRKYGLAAD+V+DAYLVDA+G+ HDRESMGEDLFWA+RGGGGGSFGIVVAWKV
Sbjct: 184 GGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 243

Query: 247 KLVPVPATVTICSTGRNLEEDVIELIYRWQHVANKLDENLFLGIILTGGK-------TKP 306
           KLV VPA VT+CST R++E+D I+LI+RWQ+VAN+LD+NLFLGI+LTGG        T P
Sbjct: 244 KLVMVPAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTREGITNP 303

Query: 307 TATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESALI--LGIEIGQPLEALLN 366
           TATFFSL+LGK+DEL+TI K  F  LGL KKDC+E+SWI+S LI   G++  +PLEALLN
Sbjct: 304 TATFFSLYLGKLDELITILKNNFSELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLN 363

Query: 367 RTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGRMDQISESEIP 426
           RTPPT   TK KSDYVK+PI  A I+GIW+RLK+ DIEVPQ++F+PYGGRM QISESE P
Sbjct: 364 RTPPTIESTKFKSDYVKDPIPEATIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETP 423

Query: 427 FPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWIRELYNYMAPFVSKLPRGAYANYRDLDIG 486
           F HRAGNLYKI YAV WKE S+D   KH+ WIRE+Y YM PFVSK PR AYANYRDLDIG
Sbjct: 424 FSHRAGNLYKIGYAVSWKEQSLDAKNKHINWIREVYEYMTPFVSKSPRAAYANYRDLDIG 483

Query: 487 SNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIP 504
           +NNKYGKT Y +ASVWG KYFG NFNRLVYVKTKVDP+DFF+HEQSIP
Sbjct: 484 TNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

BLAST of Spg035891 vs. ExPASy TrEMBL
Match: A0A5A7TU57 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00190 PE=3 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 6.8e-218
Identity = 384/533 (72.05%), Postives = 432/533 (81.05%), Query Frame = 0

Query: 3   SSPLVPLSLAFILLSSL---------------------SSVNSSISKVVYTQINSSYSSV 62
           SS L+PLSL FI++SS                      SS +  ISKVVYT INSSYSSV
Sbjct: 4   SSTLIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSYSSV 63

Query: 63  LNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFEGLSYVA 122
           L+FSIRNL FS   TPKPL+IITPSHVSHIQAAVICSK+H LQIRTRSGGHDFEGLSYVA
Sbjct: 64  LDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVA 123

Query: 123 HLPFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGVCPTVGVGG 182
           + PFI+VDLINL S+ V+VENNTAWV+SGATLGELYYRI EK+RTLAF AGVCPTVG+GG
Sbjct: 124 YRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGG 183

Query: 183 HFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGGGSFGIVVA 242
           HFSGGGYGLMLRK+GLAAD+VIDAYLVDA+GK  D+ESMGEDLFWA+RGGGGGSFGIVVA
Sbjct: 184 HFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVA 243

Query: 243 WKVKLVPVPATVTICSTGRNLEEDVIELIYRWQHVANKLDENLFLGIILTGGK------- 302
           WK+KLVPVP TVTICST RNLEED I+LI+RWQ+V NKLDEN++LGIILTGG        
Sbjct: 244 WKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQAGI 303

Query: 303 TKPTATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESALI--LGIEIGQPLEA 362
           T PTA FFSLFLG+ DELVT   T FP LGL K+DC+E SW+ES LI  +G++  + LE 
Sbjct: 304 TNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEP 363

Query: 363 LLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGRMDQISES 422
           LLNRT  T   TKIKSDY+ EPI +AAIK IW+RLK+ DIE  QL+FVPYGGRM QISES
Sbjct: 364 LLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQISES 423

Query: 423 EIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWIRELYNYMAPFVSKLPRGAYANYRDL 482
           E PF HRA  L+KI Y VGWK+ S+   K+H+ W+RE+Y YMAPFVSK PR AY NYRDL
Sbjct: 424 ETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNYRDL 483

Query: 483 DIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIPAL 506
           DIGSNNKYGKT Y+RASVWG KYFG NF+RLVYVKTKVDP DFF+HEQSIP L
Sbjct: 484 DIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Spg035891 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 515.8 bits (1327), Expect = 4.0e-146
Identity = 259/494 (52.43%), Postives = 349/494 (70.65%), Query Frame = 0

Query: 12  AFILLSSLSSVNSSI-SKVVYTQINSSYSSVLNFSIRNLRFSTANTPKPLVIITPSHVSH 71
           +F+   SL   +S+I SKV++T  ++S+SSVL  SI+N RFS  + PKP++I+TP   S 
Sbjct: 36  SFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSD 95

Query: 72  IQAAVICSKTHNLQIRTRSGGHDFEGLSYVAHLPFIIVDLINLSSITVDVENNTAWVQSG 131
           +Q+AV C++   + IRTRSGGHD+EGLSYV H PF+I+DL NL SITVDV+N + WVQ+G
Sbjct: 96  VQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTG 155

Query: 132 ATLGELYYRIAEKNRTLAFLAGVCPTVGVGGHFSGGGYGLMLRKYGLAADHVIDAYLVDA 191
           AT+GELYY I +KNRTLAF AGVCPTVGVGGHFSGGGYG +LRK+GLAADHVIDA +VDA
Sbjct: 156 ATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDA 215

Query: 192 DGKFHDRESMGEDLFWAVRGGGGGSFGIVVAWKVKLVPVPATVTICSTGRNLEEDVIELI 251
            G+  +R  MGED FWA+RGGGG SF +V++WK+ L+ VP+TVT+ +  +  E+  +++I
Sbjct: 216 RGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKII 275

Query: 252 YRWQHVANKLDENLFLGIILTGGKTKPTATFFSLFLGKIDELVTIFKTTFPALGLAKKDC 311
           +RWQ VA+K+ ++LF+ ++L   K    A+F  L+LG +  L+ +    FP LGL + DC
Sbjct: 276 HRWQFVADKVSDDLFIRVMLQRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDC 335

Query: 312 METSWIESALILGIEIG-QPLEALLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKS 371
            E SWIES +I   E+G +P+  L  RT   S   K KSD+V+EP+   AI  +W RL+ 
Sbjct: 336 TEMSWIES-VIWFAELGEEPINVLTKRT-RASLAFKAKSDFVQEPMPKTAISKLWRRLQE 395

Query: 372 HDIEVPQLIFVPYGGRMDQISESEIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWIRE 431
            + E  QLIF P+GG+M +I++ E PFPHR GN+Y+I Y   W+    DV +K++RW+  
Sbjct: 396 PEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVER 455

Query: 432 LYNYMAPFVSKLPRGAYANYRDLDIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTK 491
           +Y+ M+ FV+K PRGAY N RDLD+G      ++ Y     WG KYF  NF RLV VKT 
Sbjct: 456 VYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTS 515

Query: 492 VDPNDFFQHEQSIP 504
           VDP+DFF  EQSIP
Sbjct: 516 VDPSDFFCDEQSIP 524

BLAST of Spg035891 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 501.1 bits (1289), Expect = 1.0e-141
Identity = 247/482 (51.24%), Postives = 337/482 (69.92%), Query Frame = 0

Query: 36  SSYSSVLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFE 95
           S +S VL  + +NLRF   + PKP  I +P H SH+QA++ICSK   + +R RSGGHD+E
Sbjct: 61  SMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYE 120

Query: 96  GLSYVAHL--PFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGV 155
           GLSYV+ +  PFI++DL  +  + +++++N+AWVQSGAT+GELYYRIAEK++   F AG+
Sbjct: 121 GLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGL 180

Query: 156 CPTVGVGGHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGG 215
           C ++G+GGH +GG YG M+RKYGL AD+V+DA +VDA+GK  DR +MGED FWA+RGG G
Sbjct: 181 CSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAG 240

Query: 216 GSFGIVVAWKVKLVPVPATVTICSTGRNLEEDV-IELIYRWQHVANKLDENLFLGIIL-- 275
           GSFGI++AWK+KLVPVP TVT+ +  + L++DV  ++I +WQ VA+KL E LF+ ++   
Sbjct: 241 GSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNV 300

Query: 276 --TGGKTKPTATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIES-ALILGIEIG 335
             TGG    T ++ +LFLG    L+ + K +FP LGL  KDC+E SW+ES A I G    
Sbjct: 301 AGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTH 360

Query: 336 QPLEALL-NRTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGRM 395
            P   LL  ++P      K KSD+VK PI  + ++GI+++L   DI  P +I+ PYGG M
Sbjct: 361 TPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGMM 420

Query: 396 DQISESEIPFPHRAGNLYKIAYAVGWKEGSIDVDK---KHLRWIRELYNYMAPFVSKLPR 455
            +I ES+IPFPHR G L+K+ Y   W    +D DK   +H+ WIR+LY+YM P+VS  PR
Sbjct: 421 AKIPESQIPFPHRKGVLFKVQYVTSW----LDSDKRPSRHINWIRDLYSYMTPYVSSNPR 480

Query: 456 GAYANYRDLDIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIP 506
            AY NYRDLD+G N K  KT  ++A VWG  YF  NFNRL+ +K KVDP +FF+HEQSIP
Sbjct: 481 EAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 536

BLAST of Spg035891 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 497.7 bits (1280), Expect = 1.1e-140
Identity = 258/526 (49.05%), Postives = 349/526 (66.35%), Query Frame = 0

Query: 6   LVPLSLAFILLSSLSSVNSS---------------ISKVVYTQINSSYSSVLNFSIRNLR 65
           LV   + FI  SS SS NS                IS  ++   N SYSSVL  +IRNLR
Sbjct: 7   LVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLR 66

Query: 66  FSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFEGLSYVAH--LPFIIV 125
           F+T +TPKP +II  +H SH+QAA+ C K HNLQ++ RSGGHD++GLSYV +   PF ++
Sbjct: 67  FNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVL 126

Query: 126 DLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGVCPTVGVGGHFSGGGY 185
           D+ NL S+ VDV + TAWVQ+GA LGE+YY I EK++TLA+ AG+CPTVGVGGH SGGGY
Sbjct: 127 DMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGY 186

Query: 186 GLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGGGSFGIVVAWKVKLVP 245
           G M+RKYGL  D+ IDA +VD +GK  DR+ MGEDL+WA+ GGGGGS+G+V+A+K+ LV 
Sbjct: 187 GNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVE 246

Query: 246 VPATVTICSTGRNLEEDVIELIYRWQHVANKLDENLFLGIIL-------TGGKTKPTATF 305
           VP  VT+    R LE++  ++I+RWQ VA KL + LF+  ++       +  KT  T TF
Sbjct: 247 VPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRT-TF 306

Query: 306 FSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESALI-LGIEIGQPLEALLNRTPPT 365
            ++FLG    L++I    FP LGL + DC ETSWI+S L    I++G     LL R  P 
Sbjct: 307 IAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRNQPV 366

Query: 366 SGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGRMDQISESEIPFPHRA 425
           +   K KSDYV+EPIS   ++ IW+  K  ++E+P + F PYGG M +IS +  PFP+RA
Sbjct: 367 N-YLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTVTPFPYRA 426

Query: 426 GNLYKIAYAVGWKEGSIDVDKKHLRWIRELYNYMAPFVSKLPRGAYANYRDLDIGSNNKY 485
           GNL+KI Y   W++ ++    +++   R+LY +M PFVSK PR ++ NYRD+D+G N+  
Sbjct: 427 GNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHN 486

Query: 486 GK-TGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIPAL 506
           GK + Y     +G KYF  NF RLV +KT+VD  +FF++EQSIP L
Sbjct: 487 GKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Spg035891 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 493.0 bits (1268), Expect = 2.8e-139
Identity = 239/490 (48.78%), Postives = 340/490 (69.39%), Query Frame = 0

Query: 22  VNSSISKVVYTQINSSYSSVLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTH 81
           +N   S++++T  + SY S+LN SI+N RF    TPKP+ IITP   + +Q+ + C++ H
Sbjct: 45  INEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLH 104

Query: 82  NLQIRTRSGGHDFEGLSYVA-HLPFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRI 141
            + IRTRSGGHD+EGLSY+A   PF+++DL NL SIT+DV+N T WVQSGAT+GELYY I
Sbjct: 105 GIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEI 164

Query: 142 AEKNRTLAFLAGVCPTVGVGGHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESM 201
            + +++LAF AG+ PTVG+GG F GGGYG ++RKYGL+AD+VIDA++VDA+G F DR+ M
Sbjct: 165 GKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGM 224

Query: 202 GEDLFWAVRGGGGGSFGIVVAWKVKLVPVPATVTICSTGRNLEEDVIELIYRWQHVANKL 261
           GED FWA+RGGGG SF +V++WK++L+ VP+ VT+    +  E++ + +I +WQ++A+K+
Sbjct: 225 GEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKV 284

Query: 262 DENLFLGIILTGGKTKPTATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESAL 321
             +LF+  +L   +T+  A+F  L+LG + +L+ + K  FP LGL   +C E SWIES  
Sbjct: 285 PNDLFIRAMLQ-KETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIES-- 344

Query: 322 ILGIEIGQPLEALLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFV 381
           +L    G+ +E L  R   TS   K K D+++EPI   AI+ +W R ++ +  + ++I  
Sbjct: 345 VLWFIKGESMEILAKR-KRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILT 404

Query: 382 PYGGRMDQISESEIPFPHRAGNLYKIAYAVGWKEGSIDVDK----KHLRWIRELYNYMAP 441
           P+GG+M +I+++EIPFPHR GNLY+I Y   W E   D +K    K+LRW+  +Y +M P
Sbjct: 405 PFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEE-DKNKTNTEKYLRWVESVYEFMTP 464

Query: 442 FVSKLPRGAYANYRDLDIGSNNKYG-KTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDF 501
           +VSK PR AY N+RD+D+G       KT Y  A VWG KYF  NF+RLV VKT VDP DF
Sbjct: 465 YVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDF 524

Query: 502 FQHEQSIPAL 506
           F  EQSIP +
Sbjct: 525 FCDEQSIPIM 529

BLAST of Spg035891 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 488.8 bits (1257), Expect = 5.3e-138
Identity = 238/478 (49.79%), Postives = 327/478 (68.41%), Query Frame = 0

Query: 36  SSYSSVLNFSIRNLRFSTANTPKPLVIITPSHVSHIQAAVICSKTHNLQIRTRSGGHDFE 95
           SS+   L  S +NLR+   + PKP  I  P + +H+QAAV+C+K   L +R RSGGHD+E
Sbjct: 61  SSFKLALETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYE 120

Query: 96  GLSYVAHL--PFIIVDLINLSSITVDVENNTAWVQSGATLGELYYRIAEKNRTLAFLAGV 155
           GLSYV+ +   F+IVDL  L  I+VD+E+N+AWV +GA++GE+YYRI EK++   F AG+
Sbjct: 121 GLSYVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGL 180

Query: 156 CPTVGVGGHFSGGGYGLMLRKYGLAADHVIDAYLVDADGKFHDRESMGEDLFWAVRGGGG 215
           C ++G+GGH  GG YG M+RK+GL AD+V+DA +VDADGK  +R +MGED+FWA+RGGGG
Sbjct: 181 CTSLGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGG 240

Query: 216 GSFGIVVAWKVKLVPVPATVTICSTGRNLEEDVIELIYRWQHVANKLDENLFLGIILTGG 275
           GSFG+++AWK+KLVPVP  VT+ +  R LE+D  +L+Y+WQ VA+KLDE+LF+ +I+   
Sbjct: 241 GSFGVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPT 300

Query: 276 KTKP-------TATFFSLFLGKIDELVTIFKTTFPALGLAKKDCMETSWIESAL-ILGIE 335
              P       + ++   FLG  + L+ + + +FP LGL KKDC+ETSWI+S + I G  
Sbjct: 301 SKTPKSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFP 360

Query: 336 IGQPLEALLNRTPPTSGRTKIKSDYVKEPISMAAIKGIWERLKSHDIEVPQLIFVPYGGR 395
              P EALL+         K KSDYV+EPI +  ++G+WE+L   D   P  I+ PYGG 
Sbjct: 361 STAPSEALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEED--SPLTIWNPYGGM 420

Query: 396 MDQISESEIPFPHRAGNLYKIAYAVGWKEGSIDVDKKHLRWIRELYNYMAPFVSKLPRGA 455
           M +I E+E PFPHR+G L+KI +   W++G    + KH+ W+RE+Y+YM  +VSK PR A
Sbjct: 421 MAKIPETETPFPHRSGTLFKIQWLTLWQDGKTS-EAKHMGWMREMYSYMEQYVSKSPRSA 480

Query: 456 YANYRDLDIGSNNKYGKTGYRRASVWGFKYFGKNFNRLVYVKTKVDPNDFFQHEQSIP 504
           Y NYRDLD+G N K        A  WG +YF  NF RLV +K K DP +FF+HEQSIP
Sbjct: 481 YVNYRDLDLGMNGKGSD-----AREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530