Spg035864 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg035864
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	gibberellin 2-beta-dioxygenase-like
Location	scaffold5: 39876319 .. 39878481 (-)
RNA-Seq Expression	Spg035864
Synteny	Spg035864

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCTTTTCTATTTGAATCAAAAAGATAAACATCGATTTGAAAAAAATAAAAATAATAATAATAATGAAAAGAATTTAAAAAAGAAAAAAAAAAACCAACTCAGCCCTGATGGTATAAATACCCAACGGAAGCAAAGGCTAATGCCTCCTCATCCACTTCCTCATTCAAAATTCAAAACCATTTCTTCTTCTTCTGTTCTTGAAATCTTCTTCTTCTTCAATCATGGTGGTTCAATCCCAGCTCGTGTTGGACCATTTTGCTCCGATCAAATCCTGCAAACGCACCGCCCTGTTCACCGGAATTCCGGTGATCGACCTTTCGGACCCAGAAGCCAAAACCCATATGGTAAAAGCCTGTGAGGATTTCGGTTTCTTCAAGGTCGTCAACCACGGCGTCTCCGACGACCTCATCGCCGCCCTGGAAGCCCAAGCTCTCAAATTCTTCCAACTCCCTCAATCGGAGAAGGAAAAAGCCGGGCCACCCGACCCATTTGGATACGGCAGCAAGAGAATTGGCCGAAACGGAGATGTGGGTTGGATCGAATACCTCCTCCTCAACACCAATCACGAACTCATTTCCGAAAAATCCCTCTTCATTCTCCGGGAAAATCCCGAGATTTTCCGGTAAGACTGAATAAAACAAAAAAAAAAAAAACAGAGCACGACAGAGCCAGTGCTCTGTTTCTTTTGCTCTGTTTTTCTGATTTGTTTATCATGGTTTGTTTGTTGTAGGTGTGCGGTGGAGGAGTACATATTGGCAGTGAAGGAGATGGCTTGTGAAGTGCTGGAAGTGATGGCGGAAGGGTTGGGGATAGAGAAAAGCGCTTTGAGCAGGCTGTTGAAGGACGAGAGAAGTGACTGTTGTTTCAGGCTGAACCATTATCCGCCATGCCCGGAGCTTGAAGCTTTGAGTGGCAGGAATTTGATTGGGTTTGGGGAACACACAGACCCACAAATAATATCTGTCTTGAGATCCAATAACACAAGCGGACTCCATATCTGTTTGAGAGATGGGAGTTGGGTTTCAGTCCCACCTGATCAGACCTCCTTTTTCATCAATGTTGGTGATTCATTGCAGGTACAATCCCCTCTCTCTTTCTCTTGAACAAATGAAAATGAAGCTCTTTGATTTTCATTGATGTTTTGTTTTCAAAAACTAGCTACCCTTTTTTTCTTGGAAACTTCTTAAGATGGGTAGATGGAGGATCATTTTCTTTTATGTCAAAACAAAACAGGGTTTATTTTAAGGGGGAAATTGTGGGTTTTGATTATGTGTGTTTTGTGTTGGTGTGCAGGTGATGACTAATGGGAGGTTTAAGAGTGTAAAGCACAAGGTTTTAGCTGATCCAATAAAATCAAGGTTTTCAATGATCTACTTTGGTGGGCCACCTTTGAATGAAAAGATTGCACCTTTACCATCACTATTGGAGGAGGGAGAGCAAAGTTTGTACAGAGAATTCACTTGGTGGGATTACAAAATCTCTGCATATAAATCAAGACTTGCTGATTGTAGGTTAACCCCTTTTGAGATTAAAAATCTCACCCCTTTGTTGACCTAAGATTAGAAACCCCACAAAAGAAAAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAACACTTCCTTTCAAAATTATGGATATTTTGAAAATATGTGAAATTCTTCTGTCATTTCTCTGTTATTCTTTTATGTGGTTGTGGTTATGGTTTGGAGAGGGAGAAAAAAAAGATGTGTAAATCATAATCATATGATAAAATAATGGAGGAATAATAGGGGAATAATATTTGGCTTAAAAAAGAAAGAAAGCAAAAAAGAGTTTAAATTAGATTAGAGAGGAAAGGTAGAGATAGAGATGGTAGGGATTTGGGAAGGTTAGGAAACAAATAGAATAGGGTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTCTGTTTCTCACAAAGTTTGTTCTTTTTGTTAAATATTTTGCTTTTGAGTTTAAATGTAAGAGTGTGAGTGTGAGGACAGCCACCTTCCATCCTCCATTCCCATTAAATACCCCTTTGTAGTTTCCCCAAAATACCCCTCACTTGTAAATATACTTGTCACATGACTTCCCCTTACCTTTTTGTATCAAAGCTTGTGGATTTCAATGTTATCCATGGTTCTTACAATTTCCCATATCTCAAAAT

mRNA sequence

ATGGTGGTTCAATCCCAGCTCGTGTTGGACCATTTTGCTCCGATCAAATCCTGCAAACGCACCGCCCTGTTCACCGGAATTCCGGTGATCGACCTTTCGGACCCAGAAGCCAAAACCCATATGGTAAAAGCCTGTGAGGATTTCGGTTTCTTCAAGGTCGTCAACCACGGCGTCTCCGACGACCTCATCGCCGCCCTGGAAGCCCAAGCTCTCAAATTCTTCCAACTCCCTCAATCGGAGAAGGAAAAAGCCGGGCCACCCGACCCATTTGGATACGGCAGCAAGAGAATTGGCCGAAACGGAGATGTGGGTTGGATCGAATACCTCCTCCTCAACACCAATCACGAACTCATTTCCGAAAAATCCCTCTTCATTCTCCGGGAAAATCCCGAGATTTTCCGGTGTGCGGTGGAGGAGTACATATTGGCAGTGAAGGAGATGGCTTGTGAAGTGCTGGAAGTGATGGCGGAAGGGTTGGGGATAGAGAAAAGCGCTTTGAGCAGGCTGTTGAAGGACGAGAGAAGTGACTGTTGTTTCAGGCTGAACCATTATCCGCCATGCCCGGAGCTTGAAGCTTTGAGTGGCAGGAATTTGATTGGGTTTGGGGAACACACAGACCCACAAATAATATCTGTCTTGAGATCCAATAACACAAGCGGACTCCATATCTGTTTGAGAGATGGGAGTTGGGTTTCAGTCCCACCTGATCAGACCTCCTTTTTCATCAATGTTGGTGATTCATTGCAGGTGATGACTAATGGGAGGTTTAAGAGTGTAAAGCACAAGGTTTTAGCTGATCCAATAAAATCAAGGTTTTCAATGATCTACTTTGGTGGGCCACCTTTGAATGAAAAGATTGCACCTTTACCATCACTATTGGAGGAGGGAGAGCAAAGTTTGTACAGAGAATTCACTTGGTGGGATTACAAAATCTCTGCATATAAATCAAGACTTGCTGATTGTAGGTTAACCCCTTTTGAGATTAAAAATCTCACCCCTTTGTTGACCTAA

Coding sequence (CDS)

ATGGTGGTTCAATCCCAGCTCGTGTTGGACCATTTTGCTCCGATCAAATCCTGCAAACGCACCGCCCTGTTCACCGGAATTCCGGTGATCGACCTTTCGGACCCAGAAGCCAAAACCCATATGGTAAAAGCCTGTGAGGATTTCGGTTTCTTCAAGGTCGTCAACCACGGCGTCTCCGACGACCTCATCGCCGCCCTGGAAGCCCAAGCTCTCAAATTCTTCCAACTCCCTCAATCGGAGAAGGAAAAAGCCGGGCCACCCGACCCATTTGGATACGGCAGCAAGAGAATTGGCCGAAACGGAGATGTGGGTTGGATCGAATACCTCCTCCTCAACACCAATCACGAACTCATTTCCGAAAAATCCCTCTTCATTCTCCGGGAAAATCCCGAGATTTTCCGGTGTGCGGTGGAGGAGTACATATTGGCAGTGAAGGAGATGGCTTGTGAAGTGCTGGAAGTGATGGCGGAAGGGTTGGGGATAGAGAAAAGCGCTTTGAGCAGGCTGTTGAAGGACGAGAGAAGTGACTGTTGTTTCAGGCTGAACCATTATCCGCCATGCCCGGAGCTTGAAGCTTTGAGTGGCAGGAATTTGATTGGGTTTGGGGAACACACAGACCCACAAATAATATCTGTCTTGAGATCCAATAACACAAGCGGACTCCATATCTGTTTGAGAGATGGGAGTTGGGTTTCAGTCCCACCTGATCAGACCTCCTTTTTCATCAATGTTGGTGATTCATTGCAGGTGATGACTAATGGGAGGTTTAAGAGTGTAAAGCACAAGGTTTTAGCTGATCCAATAAAATCAAGGTTTTCAATGATCTACTTTGGTGGGCCACCTTTGAATGAAAAGATTGCACCTTTACCATCACTATTGGAGGAGGGAGAGCAAAGTTTGTACAGAGAATTCACTTGGTGGGATTACAAAATCTCTGCATATAAATCAAGACTTGCTGATTGTAGGTTAACCCCTTTTGAGATTAAAAATCTCACCCCTTTGTTGACCTAA

Protein sequence

MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSDDLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIEKSALSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSFFINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKISAYKSRLADCRLTPFEIKNLTPLLT

Homology

[Close]

BLAST of Spg035864 vs. NCBI nr
Match: XP_038893915.1 (gibberellin 2-beta-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 652.9 bits (1683), Expect = 1.5e-183
Identity = 315/336 (93.75%), Postives = 321/336 (95.54%), Query Frame = 0


Query: 1   MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSD 60
           MVVQSQLV DHFAPIKSCKRTALFTGIPVIDL DPEAKTH+VKACEDFGFFKV NHGVSD
Sbjct: 32  MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSD 91

Query: 61  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 120
           DLI ALEAQALKFF LPQSEKEKAGPPDPFGYGSKRIG NGDVGWIEY+LLNTN +LISE
Sbjct: 92  DLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISE 151

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIEKSALSRLLKDERSDCCFR 180
           KS  I RENPEIFRC VEEYILAVKEMACEVLEVMAEGL IEKSALSRLLKDERSDCCFR
Sbjct: 152 KSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIEKSALSRLLKDERSDCCFR 211

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNT+GLHICLRDG+WVSVPPD TSF
Sbjct: 212 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 271

Query: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLE+GEQSL
Sbjct: 272 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSL 331

Query: 301 YREFTWWDYKISAYKSRLADCRLTPFEIKNLTPLLT 337
           YREFTWWDYK SAYKSRLADCRL PFEIKNLTPLLT
Sbjct: 332 YREFTWWDYKNSAYKSRLADCRLGPFEIKNLTPLLT 367

[Close]

BLAST of Spg035864 vs. NCBI nr
Match: XP_023001443.1 (gibberellin 2-beta-dioxygenase-like [Cucurbita maxima] >CBL59328.1 Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 649.8 bits (1675), Expect = 1.3e-182
Identity = 313/336 (93.15%), Postives = 320/336 (95.24%), Query Frame = 0


Query: 1   MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSD 60
           MVVQSQLV DHFAPIKSCKRTALFTGIPVIDL DPEAKT +VKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 120
            LI  LEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIG NGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVKEMACEVLE MA GL IEKSALSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT+GLHICLRDG+WVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FINVGDSLQVMTNGRFKSVKHKVLADPIKSR SMIYFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKISAYKSRLADCRLTPFEIKNLTPLLT 337
           YREFTWWDYK SAYKSRLADCRL PFEIK LTPLLT
Sbjct: 301 YREFTWWDYKTSAYKSRLADCRLGPFEIKTLTPLLT 336

[Close]

BLAST of Spg035864 vs. NCBI nr
Match: KAG7019518.1 (Gibberellin 2-beta-dioxygenase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 646.7 bits (1667), Expect = 1.1e-181
Identity = 311/336 (92.56%), Postives = 320/336 (95.24%), Query Frame = 0


Query: 1   MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSD 60
           MVVQSQLV DHFAPIKSCKRTALFTGIPVI+L DPEAKT +VKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 120
            LI  LEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIG NGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPE+FRCAVEEYILAVKEMACEVLE MA GL IEKSALSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEVFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT+GLHICLRDG+WVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FINVGDSLQVMTNGRFKSVKHKVLADPIKSR SMIYFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKISAYKSRLADCRLTPFEIKNLTPLLT 337
           YREFTWWDYK SAYKSRLADCRL PFEIK LTPLLT
Sbjct: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIKTLTPLLT 336

[Close]

BLAST of Spg035864 vs. NCBI nr
Match: XP_022927050.1 (gibberellin 2-beta-dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 645.6 bits (1664), Expect = 2.4e-181
Identity = 311/336 (92.56%), Postives = 319/336 (94.94%), Query Frame = 0


Query: 1   MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSD 60
           MVVQSQLV DHFAPIKSCKRTALFTGIPVIDL DPEAKT +VKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 120
            LI  LEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIG NGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPE+FRCAVEEYILAVKEMACEVLE MA GL IEKSALS LLKDERSDCCFR
Sbjct: 121 KSLFILRENPEVFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSWLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT+GLHICLRDG+WVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FINVGDSLQVMTNGRFKSVKHKVLADPIKSR SMIYFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKISAYKSRLADCRLTPFEIKNLTPLLT 337
           YREFTWWDYK SAYKSRLADCRL PFEIK LTPLLT
Sbjct: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIKTLTPLLT 336

[Close]

BLAST of Spg035864 vs. NCBI nr
Match: XP_023542402.1 (gibberellin 2-beta-dioxygenase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645.6 bits (1664), Expect = 2.4e-181
Identity = 312/336 (92.86%), Postives = 317/336 (94.35%), Query Frame = 0


Query: 1   MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSD 60
           MVVQSQLV DHFAPIKSCK TALFTGIPVIDLSDPEAKTH+VKACEDFGFFKVVNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60

Query: 61  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 120
           DLI ALEAQALKFF LPQSEKEKAGP DPFGYGSKRIG NGDVGWIEYLLLNTNHELISE
Sbjct: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVK+MACEVLE MAEGLGIEK  LSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNT+GLHICLRDGSWV VPPDQ SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FINVGDSLQVMTNGRF SVKHKVLADP K RFSMIYFGGPPLNEKIAPL SLLEEGEQ L
Sbjct: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300

Query: 301 YREFTWWDYKISAYKSRLADCRLTPFEIKNLTPLLT 337
           Y+EFTWWDYK SAYKSRLA CRL PFE+KNLTPLLT
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336

[Close]

BLAST of Spg035864 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.6e-135
Identity = 235/328 (71.65%), Postives = 274/328 (83.54%), Query Frame = 0


Query: 1   MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSD 60
           MVV SQ  L+ F  +K  K T LFTGIPV+DL+ P+AK  +V AC DFGFFK+VNHGV  
Sbjct: 1   MVVLSQPALNQFFLLKPFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGVPL 60

Query: 61  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 120
           +L+A LE +AL+FF+  QSEK++AGPPDPFGYGSKRIG NGDVGW+EYLLLNTN ++IS 
Sbjct: 61  ELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISP 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGI-EKSALSRLLKDERSDCCF 180
           KSL I RENP  FR  VE YI AVK M   VLE+MAEGLGI +++ LSRLLKDE+SD CF
Sbjct: 121 KSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCF 180

Query: 181 RLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTS 240
           RLNHYPPCPE++AL+ RNL+GFGEHTDPQIISVLRSN+TSGL ICL DG+WVSVPPDQTS
Sbjct: 181 RLNHYPPCPEVQALN-RNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTS 240

Query: 241 FFINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQS 300
           FFINVGD+LQVMTNGRFKSVKH+VLAD  KSR SMIYFGGP L+E IAPLPS++ +GE+ 
Sbjct: 241 FFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKGEEC 300

Query: 301 LYREFTWWDYKISAYKSRLADCRLTPFE 328
           LY+EFTW +YK +AY SRLAD RL PF+
Sbjct: 301 LYKEFTWCEYKKAAYTSRLADNRLAPFQ 327

[Close]

BLAST of Spg035864 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.1e-115
Identity = 202/328 (61.59%), Postives = 260/328 (79.27%), Query Frame = 0


Query: 1   MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSD 60
           MV+ S+   + +  +++       + IP++DLS P+AKT +VKACEDFGFFKV+NHG+  
Sbjct: 1   MVLLSKPTSEQYTYVRNNMPITFSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGIPL 60

Query: 61  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 120
           D I+ LE++A KFF LPQ+EKEKAGP +PFGYG+KRIG NGD+GWIEYLLL TN     +
Sbjct: 61  DAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTN----QD 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIE-KSALSRLLKDERSDCCF 180
            +  +  E+ + FR  +++Y  A++ MACE+L++MAEGL I+ K+  S+L+ D++SDC F
Sbjct: 121 HNFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLF 180

Query: 181 RLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTS 240
           R+NHYP CPEL A++G NLIGFGEHTDPQIIS+LRSNNTSG  I LRDGSW+SVPPD +S
Sbjct: 181 RVNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSS 240

Query: 241 FFINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQS 300
           FFINVGDSLQVMTNGRFKSV+H+VLA+ I  R SMIYF GPPL+EKIAPLPSL+ +G++S
Sbjct: 241 FFINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPSLM-KGKES 300

Query: 301 LYREFTWWDYKISAYKSRLADCRLTPFE 328
           LY+EFTW++YK S Y SRLAD RL  +E
Sbjct: 301 LYKEFTWFEYKSSTYGSRLADNRLGNYE 322

[Close]

BLAST of Spg035864 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 3.1e-115
Identity = 207/332 (62.35%), Postives = 258/332 (77.71%), Query Frame = 0


Query: 1   MVVQSQLVL--DHFAPIKSCKRTALFT--GIPVIDLSDPEAKTHMVKACEDFGFFKVVNH 60
           MVV  Q V   +H + I + K   + T   IPV++L+DPEAKT +VKACE+FGFFKVVNH
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60

Query: 61  GVSDDLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHE 120
           GV  +L+  LE +A+ FF LPQS K +AGPP+P+GYG+KRIG NGDVGWIEYLLLN N +
Sbjct: 61  GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120

Query: 121 LISEKSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIE-KSALSRLLKDERS 180
           L S K+  + R+ P+IFR +VEEY+  +KE++ +VLE++AE LGIE +  LS++L+DE+S
Sbjct: 121 LSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKS 180

Query: 181 DCCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPP 240
           D C RLNHYP   E      +  +GFGEHTDPQIISVLRSNNT+GL IC++DGSWV+VPP
Sbjct: 181 DSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPP 240

Query: 241 DQTSFFINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEE 300
           D +SFFINVGD+LQVMTNGRFKSVKH+VLAD  +SR SMIYFGGPPL++KIAPLP L+ E
Sbjct: 241 DHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPE 300

Query: 301 GEQSLYREFTWWDYKISAYKSRLADCRLTPFE 328
            +  LY+EFTW  YK SAYKS+L D RL  FE
Sbjct: 301 QDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFE 330

[Close]

BLAST of Spg035864 vs. ExPASy Swiss-Prot
Match: Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 385.2 bits (988), Expect = 7.6e-106
Identity = 189/315 (60.00%), Postives = 238/315 (75.56%), Query Frame = 0


Query: 24  FTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSDDLIAALEAQALKFFQLPQSEKEK 83
           F+ IPVID+SDPE+K  +VKACEDFGFFKV+NHGVS +L++ LE + + FF LP+SEK +
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 84  AGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILA 143
                PFGYG+ +IGRNGDVGW+EYLL+N NH+  S      L ++P  FR A+EEY  +
Sbjct: 75  VA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTS 134

Query: 144 VKEMACEVLEVMAEGLGIE-KSALSRLLKDERSDCCFRLNHYPPCP--ELEALSGRNLIG 203
           V++M  +VLE + +GLGI+ ++ LS+L+ D+ +D   RLNHYPPCP    +   G+N+IG
Sbjct: 135 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIG 194

Query: 204 FGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSFFINVGDSLQVMTNGRFKSVK 263
           FGEHTDPQIISVLRSNNTSGL I L DGSW+SVPPD TSFF NVGDSLQVMTNGRFKSV+
Sbjct: 195 FGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVR 254

Query: 264 HKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKISAYKSRLAD 323
           H+VLA+  KSR SMIYF GP L ++IAPL  L++  ++ LY EFTW +YK S Y SRL+D
Sbjct: 255 HRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSD 314

Query: 324 CRLTPFEIKNLTPLL 336
            RL  FE K +  LL
Sbjct: 315 NRLQQFERKTIKNLL 328

[Close]

BLAST of Spg035864 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 1.6e-103
Identity = 185/302 (61.26%), Postives = 228/302 (75.50%), Query Frame = 0


Query: 27  IPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSDDLIAALEAQALKFFQLPQSEKEKAGP 86
           IPVIDL+D +AKT +VKACE+FGFFKV+NHGV  DL+  LE +A+ FF L  S K+KAGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 87  PDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILAVKE 146
           PDPFGYG+KRIG NGD+GW+EY+LLN N  L S K+  I R  P IFR AVEEYI  +K 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 147 MACEVLEVMAEGLGIE-KSALSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGEHT 206
           M+ + LE++ E L IE K  LSRL+K + SD C R+NHY   PE E    +  IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHY---PEKEETPVKEEIGFGEHT 206

Query: 207 DPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSFFINVGDSLQVMTNGRFKSVKHKVLA 266
           DPQ+IS+LRSN+T GL IC++DG+WV V PD +SFF+ VGD+LQVMTNGRFKSVKH+V+ 
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 267 DPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKISAYKSRLADCRLTP 326
           +  +SR SMIYF GPPL+EKIAPL  L+ + +  LY EFTW  YK+SAYK++L D RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 325

Query: 327 FE 328
           FE
Sbjct: 327 FE 325

[Close]

BLAST of Spg035864 vs. ExPASy TrEMBL
Match: I0J1I9 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox3 PE=2 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.1e-183
Identity = 313/336 (93.15%), Postives = 320/336 (95.24%), Query Frame = 0


Query: 1   MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSD 60
           MVVQSQLV DHFAPIKSCKRTALFTGIPVIDL DPEAKT +VKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 120
            LI  LEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIG NGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVKEMACEVLE MA GL IEKSALSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT+GLHICLRDG+WVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FINVGDSLQVMTNGRFKSVKHKVLADPIKSR SMIYFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKISAYKSRLADCRLTPFEIKNLTPLLT 337
           YREFTWWDYK SAYKSRLADCRL PFEIK LTPLLT
Sbjct: 301 YREFTWWDYKTSAYKSRLADCRLGPFEIKTLTPLLT 336

[Close]

BLAST of Spg035864 vs. ExPASy TrEMBL
Match: A0A6J1EMS7 (gibberellin 2-beta-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111433993 PE=3 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 1.2e-181
Identity = 311/336 (92.56%), Postives = 319/336 (94.94%), Query Frame = 0


Query: 1   MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSD 60
           MVVQSQLV DHFAPIKSCKRTALFTGIPVIDL DPEAKT +VKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 120
            LI  LEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIG NGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPE+FRCAVEEYILAVKEMACEVLE MA GL IEKSALS LLKDERSDCCFR
Sbjct: 121 KSLFILRENPEVFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSWLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT+GLHICLRDG+WVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FINVGDSLQVMTNGRFKSVKHKVLADPIKSR SMIYFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKISAYKSRLADCRLTPFEIKNLTPLLT 337
           YREFTWWDYK SAYKSRLADCRL PFEIK LTPLLT
Sbjct: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIKTLTPLLT 336

[Close]

BLAST of Spg035864 vs. ExPASy TrEMBL
Match: A0A6J1K680 (gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490445 PE=3 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.0e-181
Identity = 311/336 (92.56%), Postives = 317/336 (94.35%), Query Frame = 0


Query: 1   MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSD 60
           MVVQSQLV DHFAPIKSCK TALFTGIPVIDLSDPEAKTH+VKACEDFGFFKVVNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60

Query: 61  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 120
           DLI ALEAQALKFF LPQSEKEKAGP DPFGYGSKRIG NGDVGWIEYLLLNTNHELISE
Sbjct: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVK+MACEVLE MAEGLGIEK  LSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNT+GLHICLRDGSWV VPPDQ SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FINVGDSLQVMTNGRF SVKHKVLADP K RFSMIYFGGPPLNEKIAPL S+LEEGEQ L
Sbjct: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASILEEGEQRL 300

Query: 301 YREFTWWDYKISAYKSRLADCRLTPFEIKNLTPLLT 337
           Y+EFTWWDYK SAYKSRLA CRL PFE+KNLTPLLT
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336

[Close]

BLAST of Spg035864 vs. ExPASy TrEMBL
Match: A0A6J1GTD6 (gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457257 PE=3 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 8.3e-180
Identity = 309/336 (91.96%), Postives = 316/336 (94.05%), Query Frame = 0


Query: 1   MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSD 60
           MVVQSQLV DHFAPIKSCK TALFTGIPVIDLSDPEAKTH+VKACEDFGFFKVVNHGVS+
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSN 60

Query: 61  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 120
           +LI ALEAQALKFF LPQSEKEKAGP DPFGYGSKRIG NGDVGWIEYLLLNTNHELISE
Sbjct: 61  ELIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIEKSALSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVK+MACEVLE MAEGLGIEK  LSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNT+GLHICLRDGSWV VPPDQ SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FINVGDSLQVMTNGRF SVKHKVLADP K RFSMIYFGGPPLNEKIAPL SLL EGEQ L
Sbjct: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLVEGEQRL 300

Query: 301 YREFTWWDYKISAYKSRLADCRLTPFEIKNLTPLLT 337
           Y+EFTWWDYK SAYKSRLA CRL PFE+KNLTPLLT
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336

[Close]

BLAST of Spg035864 vs. ExPASy TrEMBL
Match: S0BE08 (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox3 PE=2 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.4e-179
Identity = 305/336 (90.77%), Postives = 320/336 (95.24%), Query Frame = 0


Query: 1   MVVQSQLVLDHFAPIKSCKRTALFTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSD 60
           MVVQSQLV DHFAPIKSCKRTALFTGIPVI+L DPEAK  +VKACEDFGFFK+VNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINLKDPEAKIQIVKACEDFGFFKLVNHGVSD 60

Query: 61  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 120
           +LIAALE+Q LKFF LPQSEKEKAGPPDPFGYGSKRIG NGDVGWIEYLLLNTN +LIS+
Sbjct: 61  ELIAALESQTLKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIEKSALSRLLKDERSDCCFR 180
           KS+ I  ENPEIFRC VEEYI A+KEMAC+VLE+MAEGL IEKSALS+LLKDERSDCCFR
Sbjct: 121 KSISIFHENPEIFRCLVEEYISAMKEMACQVLELMAEGLKIEKSALSKLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNT+GLHICLRDG+WVSVPPD TSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 240

Query: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGE+SL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEKSL 300

Query: 301 YREFTWWDYKISAYKSRLADCRLTPFEIKNLTPLLT 337
           YREFTWWDYK SAYKSRLADCRLTPFEIKNLTPLLT
Sbjct: 301 YREFTWWDYKSSAYKSRLADCRLTPFEIKNLTPLLT 336

[Close]

BLAST of Spg035864 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 416.4 bits (1069), Expect = 2.2e-116
Identity = 207/332 (62.35%), Postives = 258/332 (77.71%), Query Frame = 0


Query: 1   MVVQSQLVL--DHFAPIKSCKRTALFT--GIPVIDLSDPEAKTHMVKACEDFGFFKVVNH 60
           MVV  Q V   +H + I + K   + T   IPV++L+DPEAKT +VKACE+FGFFKVVNH
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60

Query: 61  GVSDDLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHE 120
           GV  +L+  LE +A+ FF LPQS K +AGPP+P+GYG+KRIG NGDVGWIEYLLLN N +
Sbjct: 61  GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120

Query: 121 LISEKSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIE-KSALSRLLKDERS 180
           L S K+  + R+ P+IFR +VEEY+  +KE++ +VLE++AE LGIE +  LS++L+DE+S
Sbjct: 121 LSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKS 180

Query: 181 DCCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPP 240
           D C RLNHYP   E      +  +GFGEHTDPQIISVLRSNNT+GL IC++DGSWV+VPP
Sbjct: 181 DSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPP 240

Query: 241 DQTSFFINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEE 300
           D +SFFINVGD+LQVMTNGRFKSVKH+VLAD  +SR SMIYFGGPPL++KIAPLP L+ E
Sbjct: 241 DHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPE 300

Query: 301 GEQSLYREFTWWDYKISAYKSRLADCRLTPFE 328
            +  LY+EFTW  YK SAYKS+L D RL  FE
Sbjct: 301 QDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFE 330

[Close]

BLAST of Spg035864 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 385.2 bits (988), Expect = 5.4e-107
Identity = 189/315 (60.00%), Postives = 238/315 (75.56%), Query Frame = 0


Query: 24  FTGIPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSDDLIAALEAQALKFFQLPQSEKEK 83
           F+ IPVID+SDPE+K  +VKACEDFGFFKV+NHGVS +L++ LE + + FF LP+SEK +
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 84  AGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILA 143
                PFGYG+ +IGRNGDVGW+EYLL+N NH+  S      L ++P  FR A+EEY  +
Sbjct: 75  VA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTS 134

Query: 144 VKEMACEVLEVMAEGLGIE-KSALSRLLKDERSDCCFRLNHYPPCP--ELEALSGRNLIG 203
           V++M  +VLE + +GLGI+ ++ LS+L+ D+ +D   RLNHYPPCP    +   G+N+IG
Sbjct: 135 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIG 194

Query: 204 FGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSFFINVGDSLQVMTNGRFKSVK 263
           FGEHTDPQIISVLRSNNTSGL I L DGSW+SVPPD TSFF NVGDSLQVMTNGRFKSV+
Sbjct: 195 FGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVR 254

Query: 264 HKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKISAYKSRLAD 323
           H+VLA+  KSR SMIYF GP L ++IAPL  L++  ++ LY EFTW +YK S Y SRL+D
Sbjct: 255 HRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSD 314

Query: 324 CRLTPFEIKNLTPLL 336
            RL  FE K +  LL
Sbjct: 315 NRLQQFERKTIKNLL 328

[Close]

BLAST of Spg035864 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 377.5 bits (968), Expect = 1.1e-104
Identity = 185/302 (61.26%), Postives = 228/302 (75.50%), Query Frame = 0


Query: 27  IPVIDLSDPEAKTHMVKACEDFGFFKVVNHGVSDDLIAALEAQALKFFQLPQSEKEKAGP 86
           IPVIDL+D +AKT +VKACE+FGFFKV+NHGV  DL+  LE +A+ FF L  S K+KAGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 87  PDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILAVKE 146
           PDPFGYG+KRIG NGD+GW+EY+LLN N  L S K+  I R  P IFR AVEEYI  +K 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 147 MACEVLEVMAEGLGIE-KSALSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGEHT 206
           M+ + LE++ E L IE K  LSRL+K + SD C R+NHY   PE E    +  IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHY---PEKEETPVKEEIGFGEHT 206

Query: 207 DPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTSFFINVGDSLQVMTNGRFKSVKHKVLA 266
           DPQ+IS+LRSN+T GL IC++DG+WV V PD +SFF+ VGD+LQVMTNGRFKSVKH+V+ 
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 267 DPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKISAYKSRLADCRLTP 326
           +  +SR SMIYF GPPL+EKIAPL  L+ + +  LY EFTW  YK+SAYK++L D RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 325

Query: 327 FE 328
           FE
Sbjct: 327 FE 325

[Close]

BLAST of Spg035864 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 306.6 bits (784), Expect = 2.5e-83
Identity = 153/255 (60.00%), Postives = 196/255 (76.86%), Query Frame = 0


Query: 1   MVVQSQLVL--DHFAPIKSCKRTALFT--GIPVIDLSDPEAKTHMVKACEDFGFFKVVNH 60
           MVV  Q V   +H + I + K   + T   IPV++L+DPEAKT +VKACE+FGFFKVVNH
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60

Query: 61  GVSDDLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHE 120
           GV  +L+  LE +A+ FF LPQS K +AGPP+P+GYG+KRIG NGDVGWIEYLLLN N +
Sbjct: 61  GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120

Query: 121 LISEKSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGLGIE-KSALSRLLKDERS 180
           L S K+  + R+ P+IFR +VEEY+  +KE++ +VLE++AE LGIE +  LS++L+DE+S
Sbjct: 121 LSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKS 180

Query: 181 DCCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPP 240
           D C RLNHYP   E      +  +GFGEHTDPQIISVLRSNNT+GL IC++DGSWV+VPP
Sbjct: 181 DSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPP 240

Query: 241 DQTSFFINVGDSLQV 251
           D +SFFINVGD+LQV
Sbjct: 241 DHSSFFINVGDALQV 253

[Close]

BLAST of Spg035864 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 300.8 bits (769), Expect = 1.3e-81
Identity = 154/329 (46.81%), Postives = 208/329 (63.22%), Query Frame = 0


Query: 7   LVLDHFAPIKSCKRTALFT---GIPVIDLS---DPEAKTHMVKACEDFGFFKVVNHGVSD 66
           +VL    P+++  +  + +     PVID S     +    +VKACE  GFFKV+NHGV  
Sbjct: 1   MVLPSSTPLQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKP 60

Query: 67  DLIAALEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGRNGDVGWIEYLLLNTNHELISE 126
           ++I   E +  +FF  P+S+K +AGP  PFGYG K IG NGD+G +EYLLL+ N   +++
Sbjct: 61  EIIKRFEHEGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVAD 120

Query: 127 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEVMAEGL-GIEKSALSRLLKDERSDCCF 186
           KS  I  ++P  F  A  +YI  V+++ACE++++  E L G + S +S L++D RSD   
Sbjct: 121 KSETISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSIL 180

Query: 187 RLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTSGLHICLRDGSWVSVPPDQTS 246
           RLNHYPP P   ALSG   IGFGEH+DPQI++VLRSN+  GL IC RDG W+ +P D T 
Sbjct: 181 RLNHYPPAP--YALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTC 240

Query: 247 FFINVGDSLQVMTNGRFKSVKHKVLADPIKS-RFSMIYFGGPPLNEKIAPLPSLLEEGEQ 306
           FF+ VGD LQ +TNGRF SV+H+VLA+  K  R S +YF  PPL  KI+PLP ++     
Sbjct: 241 FFVLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENP 300

Query: 307 SLYREFTWWDYKISAYKSRLADCRLTPFE 328
             Y  FTW DYK + Y  RL   RL  F+
Sbjct: 301 RRYNSFTWGDYKKATYSLRLDVPRLEFFK 327

The following BLAST results are available for this feature:

BLAST of Spg035864 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893915.1	1.5e-183	93.75	gibberellin 2-beta-dioxygenase-like [Benincasa hispida]	[more]
XP_023001443.1	1.3e-182	93.15	gibberellin 2-beta-dioxygenase-like [Cucurbita maxima] >CBL59328.1 Gibberellin 2...	[more]
KAG7019518.1	1.1e-181	92.56	Gibberellin 2-beta-dioxygenase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022927050.1	2.4e-181	92.56	gibberellin 2-beta-dioxygenase-like [Cucurbita moschata]	[more]
XP_023542402.1	2.4e-181	92.86	gibberellin 2-beta-dioxygenase-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg035864 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XG83	4.6e-135	71.65	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
Q9SQ80	1.1e-115	61.59	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
Q9XFR9	3.1e-115	62.35	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q8LEA2	7.6e-106	60.00	Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ...	[more]
O64692	1.6e-103	61.26	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]

BLAST of Spg035864 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
I0J1I9	6.1e-183	93.15	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox3 PE=2 SV=1	[more]
A0A6J1EMS7	1.2e-181	92.56	gibberellin 2-beta-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC11143399...	[more]
A0A6J1K680	2.0e-181	92.56	gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1GTD6	8.3e-180	91.96	gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=...	[more]
S0BE08	2.4e-179	90.77	GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox3 PE=2 SV=1	[more]

BLAST of Spg035864 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30040.1	2.2e-116	62.35	gibberellin 2-oxidase	[more]
AT1G78440.1	5.4e-107	60.00	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT2G34555.1	1.1e-104	61.26	gibberellin 2-oxidase 3	[more]
AT1G30040.2	2.5e-83	60.00	gibberellin 2-oxidase	[more]
AT1G02400.1	1.3e-81	46.81	gibberellin 2-oxidase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 41..58 score: 50.51 coord: 204..225 score: 27.69 coord: 240..266 score: 29.29
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 6..330
None	No IPR available	PANTHER	PTHR47990:SF121	GIBBERELLIN 2-BETA-DIOXYGENASE 2	coord: 6..330
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 12..319
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 27..113 e-value: 7.8E-17 score: 62.1
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 4..319 e-value: 7.2E-83 score: 280.6
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 176..278 e-value: 6.3E-26 score: 90.8
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 174..280 score: 10.900123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg035864.1	Spg035864.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045487	gibberellin catabolic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0052634	C-19 gibberellin 2-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding