Spg035822 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg035822
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Endochitinase
Location	scaffold5: 39574365 .. 39576340 (-)
RNA-Seq Expression	Spg035822
Synteny	Spg035822

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTACTCCGATTCCGCAAATTAGGGCGAAATTTCTCCCCACACTTCTTCTCCTGATCGGACTCCTCGCCGGAACCGCCGTGGTGGCTCAGAATTGCGGCTGCAAAGCCGGCTCTCTTTGCTGCAGCCGATCTAGCTTCTGCGGCTCCGGCAACAAATTATGCGGATTCGGCTGCAGAGAGTTTCTGAATGCGTGGAAGCCTCTCATCTCTGTTACCGAGAATGAGATTGCTGGCGCCCCTAAGTGCGGCTGCTCCGCCGGACTCTGCTGCAGCCGGCACGGCTTCTGTGGCGTTGGCAACGGCTACTGCGGAGCCGGATGTCGAGAAGGACCGTGTAAAGCGGCTCCGGCGCCGAGTCCCGGCATGAACGGCACGGTGCTGGGCGACATCGTGACGGAAGTTTTCTTCGAGAACATTACTGGTGGAGGCGATGAGCGGTGTGCCGGAAAAAGGTTTTATAGCAGAAAGGCGTTTGTGGAAGCTGTGGAATCGTTCGATGAATTTGGTAGAATTGGTTCTGTCGATGATTCGAAGAGGGAAATTGCTGCGTTTTTCAGTCACGTCACTTATGAGTCTGGAAGTAAGATCGACTCTCTCCTTCGTACGGATTCATTTCAAATGAATTAAAAAAATAATGCTTAAATTAATTATAAAAAAAAAGTCTGAAATTTGAATTATACCTCTTAGCTTTTAAAAGTGTTTCAAAACAATTTTTTTTTTTAGACATTTTGCATTAAATTTATACATGAAAAATTATGTATTTTTATGCGTTTTTATTGTTTTTTAAGTACATCTTCACGTGCCAAAATGAGCTTAGCTCAGCGGTAATTGGCATGCATCTTCGACCATGAGCTCAGCGTTTAACCATTTTTGTGTTTATTAGATTCTGAATTTAAAAAAAAAAAAAAAAATCCTAGTCTCTAACAATTTTGCTTTCTTGAACTTAAAAATGTTAGATAAGTTTCTATGTCTTTTTTATGACCTTTTGGTTTTTAGTTTTTGAAAATTAAGCTTATAAATACTACTTTCACTTATATGTTTATTTGTTTTGTTATTTACCTTTTTTACATATTTTCAAAAATCAAGTCAAGTTTTAAAAACTAAAAAAACTAATTTTTTAAATTTAGCCATAAATTTCATCGAAGATGAAAATCATGGTAAGAAAATTTGAAAGAAAAAAAAAAAAGAAAGTTTAATTTTTTTTTAAAAGAAAAAAATCAAATGATTATCAAACGGGGCCTAAACTTTTAAGTTGGCATGGTTGATAACATATACAATATTTTTTCAAAATTAATTAATCTATTAGACATTAAATTGAAGTTTGGATAATTATGGAGCTTTATTTAACCGTCATTCTGTTTTTAAGAGTAGATTTTAAAAAATAAACATTACTTTTGTGCATAGACTTGTGCTTGATTAAAAAAGAGAGAGAGTAGTTTTTGTTTTTTATACTTGATTAACAATTGAAATGTTACATTAACGAAGAGAAAAACCATGCTAAAAAAAATAGAGAAAAACAAACATAATAATGAAAATTCCAAGTGTATTTTCTTTGTTTGTGCATAGACTTGTACTTCGTAGAAGAAATAGACGGGGAAACGGGTGACTACTGCGACAAGGAAAAGACCCAGTACCCGTGCAACCCAAGCAAGAGGTACTTCGGGCGAGGCCCAATCCAACTATCGTGGAACTTCAACTACGGCCCAGCAGGCGAGGCCATCGGCTTCGACGGCCTCAACAACCCTGAAATCGTCGCCTCCGACCCGGTGGTGTCCTTCAAGACGGCGCTCTGGTACTGGATGAACAATGTTCACTCTGTCGTCGGCCAAGGTTTTGGGGCAACCATTCGAGCCGTTAATGGTGCTGTTGAATGCGACGGTGCTAACCCGGGTGCGGTGCAACGACGAGTGATGCTCTACACTCAATACTGTGACCAACTCGGTGTTGATCCGGGCCATAATCTTACATGTTGA

mRNA sequence

ATGTCTACTCCGATTCCGCAAATTAGGGCGAAATTTCTCCCCACACTTCTTCTCCTGATCGGACTCCTCGCCGGAACCGCCGTGGTGGCTCAGAATTGCGGCTGCAAAGCCGGCTCTCTTTGCTGCAGCCGATCTAGCTTCTGCGGCTCCGGCAACAAATTATGCGGATTCGGCTGCAGAGAGTTTCTGAATGCGTGGAAGCCTCTCATCTCTGTTACCGAGAATGAGATTGCTGGCGCCCCTAAGTGCGGCTGCTCCGCCGGACTCTGCTGCAGCCGGCACGGCTTCTGTGGCGTTGGCAACGGCTACTGCGGAGCCGGATGTCGAGAAGGACCGTGTAAAGCGGCTCCGGCGCCGAGTCCCGGCATGAACGGCACGGTGCTGGGCGACATCGTGACGGAAGTTTTCTTCGAGAACATTACTGGTGGAGGCGATGAGCGGTGTGCCGGAAAAAGGTTTTATAGCAGAAAGGCGTTTGTGGAAGCTGTGGAATCGTTCGATGAATTTGGTAGAATTGGTTCTGTCGATGATTCGAAGAGGGAAATTGCTGCGTTTTTCAGTCACGTCACTTATGAGTCTGGAAACTTGTACTTCGTAGAAGAAATAGACGGGGAAACGGGTGACTACTGCGACAAGGAAAAGACCCAGTACCCGTGCAACCCAAGCAAGAGGTACTTCGGGCGAGGCCCAATCCAACTATCGTGGAACTTCAACTACGGCCCAGCAGGCGAGGCCATCGGCTTCGACGGCCTCAACAACCCTGAAATCGTCGCCTCCGACCCGGTGGTGTCCTTCAAGACGGCGCTCTGGTACTGGATGAACAATGTTCACTCTGTCGTCGGCCAAGGTTTTGGGGCAACCATTCGAGCCGTTAATGGTGCTGTTGAATGCGACGGTGCTAACCCGGGTGCGGTGCAACGACGAGTGATGCTCTACACTCAATACTGTGACCAACTCGGTGTTGATCCGGGCCATAATCTTACATGTTGA

Coding sequence (CDS)

ATGTCTACTCCGATTCCGCAAATTAGGGCGAAATTTCTCCCCACACTTCTTCTCCTGATCGGACTCCTCGCCGGAACCGCCGTGGTGGCTCAGAATTGCGGCTGCAAAGCCGGCTCTCTTTGCTGCAGCCGATCTAGCTTCTGCGGCTCCGGCAACAAATTATGCGGATTCGGCTGCAGAGAGTTTCTGAATGCGTGGAAGCCTCTCATCTCTGTTACCGAGAATGAGATTGCTGGCGCCCCTAAGTGCGGCTGCTCCGCCGGACTCTGCTGCAGCCGGCACGGCTTCTGTGGCGTTGGCAACGGCTACTGCGGAGCCGGATGTCGAGAAGGACCGTGTAAAGCGGCTCCGGCGCCGAGTCCCGGCATGAACGGCACGGTGCTGGGCGACATCGTGACGGAAGTTTTCTTCGAGAACATTACTGGTGGAGGCGATGAGCGGTGTGCCGGAAAAAGGTTTTATAGCAGAAAGGCGTTTGTGGAAGCTGTGGAATCGTTCGATGAATTTGGTAGAATTGGTTCTGTCGATGATTCGAAGAGGGAAATTGCTGCGTTTTTCAGTCACGTCACTTATGAGTCTGGAAACTTGTACTTCGTAGAAGAAATAGACGGGGAAACGGGTGACTACTGCGACAAGGAAAAGACCCAGTACCCGTGCAACCCAAGCAAGAGGTACTTCGGGCGAGGCCCAATCCAACTATCGTGGAACTTCAACTACGGCCCAGCAGGCGAGGCCATCGGCTTCGACGGCCTCAACAACCCTGAAATCGTCGCCTCCGACCCGGTGGTGTCCTTCAAGACGGCGCTCTGGTACTGGATGAACAATGTTCACTCTGTCGTCGGCCAAGGTTTTGGGGCAACCATTCGAGCCGTTAATGGTGCTGTTGAATGCGACGGTGCTAACCCGGGTGCGGTGCAACGACGAGTGATGCTCTACACTCAATACTGTGACCAACTCGGTGTTGATCCGGGCCATAATCTTACATGTTGA

Protein sequence

MSTPIPQIRAKFLPTLLLLIGLLAGTAVVAQNCGCKAGSLCCSRSSFCGSGNKLCGFGCREFLNAWKPLISVTENEIAGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFFENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLYFVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCDQLGVDPGHNLTC

Homology

[Close]

BLAST of Spg035822 vs. NCBI nr
Match: XP_038894874.1 (endochitinase EP3-like [Benincasa hispida])

HSP 1 Score: 400.6 bits (1028), Expect = 1.3e-107
Identity = 185/252 (73.41%), Postives = 203/252 (80.56%), Query Frame = 0


Query: 78  AGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFF 137
           A A  CGC+AGLCCSR GFCG G  +CG GCREGPC   P  +P +N  V+ D VTE FF
Sbjct: 25  AAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 84

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLY 197
             I    D  CAG+ FYSR  F+EA+ SFD FGRIGSVDDSKREIAAFF HVT+E+G+L 
Sbjct: 85  NGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 144

Query: 198 FVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG T DYCD+E TQYPCNPSK YFGRGPIQLSWNFNYGPAGE IGFDGLNNPEIV
Sbjct: 145 FIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGENIGFDGLNNPEIV 204

Query: 258 ASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCD 317
           A+DPVVSFKTALWYWMN V  V+ QGFGATIRA+NGA+ECDGAN   VQRRV  YTQYCD
Sbjct: 205 ATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANNATVQRRVQFYTQYCD 264

Query: 318 QLGVDPGHNLTC 330
           QLGV PG+NLTC
Sbjct: 265 QLGVAPGNNLTC 275

[Close]

BLAST of Spg035822 vs. NCBI nr
Match: KAG7019501.1 (Endochitinase EP3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 399.1 bits (1024), Expect = 3.8e-107
Identity = 182/252 (72.22%), Postives = 205/252 (81.35%), Query Frame = 0


Query: 78  AGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFF 137
           A A  CGC+AGLCCSR GFCG GN +CG GCREGPC   P  +P +N  V+ D VTE FF
Sbjct: 18  AAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 77

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLY 197
             I    D  CAG+ FYSR  F+EA++SFD FGRIGSVDDSKREIAAFF HVT+E+G+L 
Sbjct: 78  NGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 137

Query: 198 FVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG T DYCD+E TQYPCNP+K YFGRGPIQLSWNFNYGPAGE+IGFDGLNNPEIV
Sbjct: 138 FIEEIDGATKDYCDEENTQYPCNPNKGYFGRGPIQLSWNFNYGPAGESIGFDGLNNPEIV 197

Query: 258 ASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCD 317
           A++P+VSFKTALWYWMN V  V+ QGFGATIRA+NGA+ECDGAN   VQRRV  YTQYC+
Sbjct: 198 ATNPLVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANSATVQRRVQYYTQYCN 257

Query: 318 QLGVDPGHNLTC 330
           QLGV PG NLTC
Sbjct: 258 QLGVAPGDNLTC 268

[Close]

BLAST of Spg035822 vs. NCBI nr
Match: XP_023001456.1 (endochitinase EP3 [Cucurbita maxima])

HSP 1 Score: 399.1 bits (1024), Expect = 3.8e-107
Identity = 183/252 (72.62%), Postives = 204/252 (80.95%), Query Frame = 0


Query: 78  AGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFF 137
           A A  CGC+AGLCCSR GFCG GN +CG GCREGPC   P  +P +N  V+ D VTE FF
Sbjct: 18  AAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 77

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLY 197
             I    D  CAG+ FYSR  F+EA++SFD FGRIGSVDDSKREIAAFF HVT+E+G+L 
Sbjct: 78  NGILNQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 137

Query: 198 FVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG T DYCD+E TQYPCNPSK YFGRGPIQLSWNFNYGPAGE+IGFDGLNNPEIV
Sbjct: 138 FIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGESIGFDGLNNPEIV 197

Query: 258 ASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCD 317
           A++PVVSFKTALWYWMN V  V+ QGFGATIRA+NGA+ECDG N   VQRRV  YTQYC+
Sbjct: 198 ATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGGNSATVQRRVEYYTQYCN 257

Query: 318 QLGVDPGHNLTC 330
           QLGV PG NLTC
Sbjct: 258 QLGVAPGDNLTC 268

[Close]

BLAST of Spg035822 vs. NCBI nr
Match: XP_022927074.1 (endochitinase EP3 [Cucurbita moschata])

HSP 1 Score: 398.3 bits (1022), Expect = 6.5e-107
Identity = 182/252 (72.22%), Postives = 205/252 (81.35%), Query Frame = 0


Query: 78  AGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFF 137
           A A  CGC+AGLCCSR GFCG GN +CG GCREGPC   P  +P +N  V+ D VTE FF
Sbjct: 18  AAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 77

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLY 197
             I    D  CAG+ FYSR  F+EA++SFD FGRIGSVDDSKREIAAFF HVT+E+G+L 
Sbjct: 78  NGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 137

Query: 198 FVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG T DYCD++ TQYPCNPSK YFGRGPIQLSWNFNYGPAGE+IGFDGLNNPEIV
Sbjct: 138 FIEEIDGATKDYCDEDNTQYPCNPSKGYFGRGPIQLSWNFNYGPAGESIGFDGLNNPEIV 197

Query: 258 ASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCD 317
           A++P+VSFKTALWYWMN V  V+ QGFGATIRA+NGA+ECDGAN   VQRRV  YTQYC+
Sbjct: 198 ATNPLVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANSATVQRRVEYYTQYCN 257

Query: 318 QLGVDPGHNLTC 330
           QLGV PG NLTC
Sbjct: 258 QLGVAPGDNLTC 268

[Close]

BLAST of Spg035822 vs. NCBI nr
Match: KAG6583882.1 (Endochitinase EP3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 398.3 bits (1022), Expect = 6.5e-107
Identity = 182/252 (72.22%), Postives = 205/252 (81.35%), Query Frame = 0


Query: 78  AGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFF 137
           A A  CGC+AGLCCSR GFCG GN +CG GCREGPC   P  +P +N  V+ D VTE FF
Sbjct: 18  AAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 77

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLY 197
             I    D  CAG+ FYSR  F+EA++SFD FGRIGSVDDSKREIAAFF HVT+E+G+L 
Sbjct: 78  NGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 137

Query: 198 FVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG T DYCD+E TQYPCNP+K YFGRGPIQLSWNFNYGPAGE+IGFDGLNNPEIV
Sbjct: 138 FIEEIDGATKDYCDEENTQYPCNPNKGYFGRGPIQLSWNFNYGPAGESIGFDGLNNPEIV 197

Query: 258 ASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCD 317
           A++P+VSFKTALWYWMN V  V+ QGFGATIRA+NGA+ECDGAN   VQRRV  YTQYC+
Sbjct: 198 ATNPLVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANSATVQRRVEYYTQYCN 257

Query: 318 QLGVDPGHNLTC 330
           QLGV PG NLTC
Sbjct: 258 QLGVAPGDNLTC 268

[Close]

BLAST of Spg035822 vs. ExPASy Swiss-Prot
Match: Q9M2U5 (Endochitinase EP3 OS=Arabidopsis thaliana OX=3702 GN=EP3 PE=1 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.2e-98
Identity = 163/250 (65.20%), Postives = 193/250 (77.20%), Query Frame = 0


Query: 80  APKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFFEN 139
           A  CGCS+ LCCS+ GFCG  + YCG GC++GPC A P      NG  + +IVT+ FF  
Sbjct: 28  AQNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFAPPP----ANGVSVAEIVTQEFFNG 87

Query: 140 ITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLYFV 199
           I       CAG RFYSR AF+EA++S+  FGR+GS DDS+REIAAFF+HVT+E+G+  ++
Sbjct: 88  IISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFFAHVTHETGHFCYI 147

Query: 200 EEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVAS 259
           EEIDG + DYCD+  TQYPCNP+K Y+GRGPIQLSWNFNYGPAG AIGFDGLN PE VA+
Sbjct: 148 EEIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVAT 207

Query: 260 DPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCDQL 319
           DPV+SFKTALWYW N V  V+ QGFGATIRA+NGA+ECDGAN   VQ RV  YT YC QL
Sbjct: 208 DPVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTATVQARVRYYTDYCRQL 267

Query: 320 GVDPGHNLTC 330
           GVDPG+NLTC
Sbjct: 268 GVDPGNNLTC 273

[Close]

BLAST of Spg035822 vs. ExPASy Swiss-Prot
Match: P27054 (Endochitinase PR4 OS=Phaseolus vulgaris OX=3885 GN=CHI4 PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 9.8e-90
Identity = 155/252 (61.51%), Postives = 186/252 (73.81%), Query Frame = 0


Query: 80  APKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPC-KAAPAPSPGMNGTVLGDIVTEVFFE 139
           A  CGC+ GLCCS++G+CG G  YCG GC++GPC  A+P PS  +N     DI+T  F  
Sbjct: 23  AQNCGCAEGLCCSQYGYCGTGEDYCGTGCQQGPCTTASPPPSNNVN----ADILTADFLN 82

Query: 140 NITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLYF 199
            I    D  CAGK FY+R AF+ A+ S+ +FGR+GS DDSKREIAA F+H T+E+G+  +
Sbjct: 83  GIIDQADSGCAGKNFYTRDAFLSALNSYTDFGRVGSEDDSKREIAAAFAHFTHETGHFCY 142

Query: 200 VEEIDGETGDYCDKEK-TQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 259
           +EEIDG + DYCD+E   QYPC+ SK Y GRGPIQLSWNFNYGPAG A  FDGL  PE V
Sbjct: 143 IEEIDGASKDYCDEESIAQYPCSSSKGYHGRGPIQLSWNFNYGPAGSANNFDGLGAPETV 202

Query: 260 ASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCD 319
           ++D VVSFKTALWYWM +V  V+ QGFGATIRA+NGA+ECDGANP  VQ RV  YT+YC 
Sbjct: 203 SNDVVVSFKTALWYWMQHVRPVINQGFGATIRAINGALECDGANPTTVQARVNYYTEYCR 262

Query: 320 QLGVDPGHNLTC 330
           QLGV  G NLTC
Sbjct: 263 QLGVATGDNLTC 270

[Close]

BLAST of Spg035822 vs. ExPASy Swiss-Prot
Match: Q7Y1Z0 (Chitinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht5 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.6e-87
Identity = 144/262 (54.96%), Postives = 181/262 (69.08%), Query Frame = 0


Query: 78  AGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPC----------KAAPAPSPGMNGTV 137
           A A  CGC + +CCS+ G+CG G  YCG GCR GPC                  G +G  
Sbjct: 27  ASAQNCGCQSNMCCSKWGYCGTGKDYCGDGCRSGPCYGGGGGGGGGGGGGGGGGGGSGVS 86

Query: 138 LGDIVTEVFFENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFS 197
           +  +VTE FF  I       CAGK FY+R++F+ A  S+  F R  + DDSKREIAAFF+
Sbjct: 87  VESVVTEAFFNGIKNQAPNGCAGKNFYTRQSFLNAAHSYSGFARDRTNDDSKREIAAFFA 146

Query: 198 HVTYESGNLYFVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIG 257
           HVT+E+G++ ++ EI+G + DYCDK   Q+PC P K+Y+GRGP+Q+SWN+NYGPAG+ IG
Sbjct: 147 HVTHETGHMCYINEINGASMDYCDKNNKQWPCQPGKKYYGRGPLQISWNYNYGPAGQNIG 206

Query: 258 FDGLNNPEIVASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQR 317
           FDGL +P+ VA DP +SFKTALW+WMNNVH V+ QGFGATIRA+NGA+EC+G NPGAV  
Sbjct: 207 FDGLRDPDRVAQDPTISFKTALWFWMNNVHQVMLQGFGATIRAINGALECNGKNPGAVNA 266

Query: 318 RVMLYTQYCDQLGVDPGHNLTC 330
           RV  Y  YC Q GVDPG NL C
Sbjct: 267 RVNYYKDYCRQFGVDPGGNLYC 288

[Close]

BLAST of Spg035822 vs. ExPASy Swiss-Prot
Match: Q6K8R2 (Chitinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht6 PE=2 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 6.0e-87
Identity = 145/263 (55.13%), Postives = 181/263 (68.82%), Query Frame = 0


Query: 69  LISVTENEIAGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVL 128
           +++      A A  CGC++  CCS+ GFCG G+ YCG GC+ GPC       P  N   +
Sbjct: 14  MVAAVSASTAAAQSCGCASDQCCSKWGFCGTGSDYCGTGCQAGPCDV-----PATNDVSV 73

Query: 129 GDIVTEVFFENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSH 188
             IVT  FF  +    D+ CA K FY+R AF+ A   +  FGR GSVDDSKREIAAFF+H
Sbjct: 74  ASIVTPEFFAALVAQADDGCAAKGFYTRDAFLTAAGGYPSFGRTGSVDDSKREIAAFFAH 133

Query: 189 VTYESGNLYFVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGF 248
             +E+    ++EEIDG + +YCD+  TQ+PC   K Y+GRGP+Q+SWNFNYGPAG++IGF
Sbjct: 134 ANHETIKFCYIEEIDGPSKNYCDETSTQWPCMAGKGYYGRGPLQISWNFNYGPAGQSIGF 193

Query: 249 DGLNNPEIVASDPVVSFKTALWYWMNNVHS--VVGQGFGATIRAVNGAVECDGANPGAVQ 308
           DGL +P+ VA  PV++F+TALWYW NNVH   V GQGFGATIRA+NGA+ECDG NP AV 
Sbjct: 194 DGLGDPDAVARSPVLAFQTALWYWTNNVHDAFVSGQGFGATIRAINGALECDGKNPTAVS 253

Query: 309 RRVMLYTQYCDQLGVDPGHNLTC 330
            RV  Y Q+C Q GVDPG NLTC
Sbjct: 254 NRVAYYQQFCQQFGVDPGSNLTC 271

[Close]

BLAST of Spg035822 vs. ExPASy Swiss-Prot
Match: O04138 (Chitinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht4 PE=2 SV=2)

HSP 1 Score: 317.0 bits (811), Expect = 2.5e-85
Identity = 144/261 (55.17%), Postives = 178/261 (68.20%), Query Frame = 0


Query: 78  AGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPC-KAAPAPSPGMNGTVLG------- 137
           A A  CGC  G CCS+ G+CG    YCG GC+ GPC  +    + GM G   G       
Sbjct: 25  AAAQNCGCQDGYCCSQWGYCGTTEAYCGQGCQSGPCWGSGGEAAAGMAGRKAGAGAGVSV 84

Query: 138 -DIVTEVFFENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSH 197
             +VTE FF  I       CAGK FY+R++F+ A  S+  F    + DDSKREIAAFF+H
Sbjct: 85  ESVVTEAFFNGIKNQAPNGCAGKSFYTRQSFLNAARSYSGFANDRTNDDSKREIAAFFAH 144

Query: 198 VTYESGNLYFVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGF 257
           VT+E+G++ ++ EI+G   DYCDK   Q+PC P K+Y+GRGP+Q+SWNFNYGPAG+ IGF
Sbjct: 145 VTHETGHMCYINEINGANMDYCDKSNKQWPCQPGKKYYGRGPLQISWNFNYGPAGKNIGF 204

Query: 258 DGLNNPEIVASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRR 317
           DGL +P+ VA DP +SFKTALW+WMNNVH V+ QGFGATIRA+NGA+EC+G NPGAV  R
Sbjct: 205 DGLRDPDKVAQDPTISFKTALWFWMNNVHQVMSQGFGATIRAINGALECNGKNPGAVNAR 264

Query: 318 VMLYTQYCDQLGVDPGHNLTC 330
           V  Y  YC Q GV PG NL C
Sbjct: 265 VNYYKDYCRQFGVSPGGNLYC 285

[Close]

BLAST of Spg035822 vs. ExPASy TrEMBL
Match: A0A6J1KIN5 (endochitinase EP3 OS=Cucurbita maxima OX=3661 GN=LOC111495584 PE=4 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.8e-107
Identity = 183/252 (72.62%), Postives = 204/252 (80.95%), Query Frame = 0


Query: 78  AGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFF 137
           A A  CGC+AGLCCSR GFCG GN +CG GCREGPC   P  +P +N  V+ D VTE FF
Sbjct: 18  AAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 77

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLY 197
             I    D  CAG+ FYSR  F+EA++SFD FGRIGSVDDSKREIAAFF HVT+E+G+L 
Sbjct: 78  NGILNQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 137

Query: 198 FVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG T DYCD+E TQYPCNPSK YFGRGPIQLSWNFNYGPAGE+IGFDGLNNPEIV
Sbjct: 138 FIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGESIGFDGLNNPEIV 197

Query: 258 ASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCD 317
           A++PVVSFKTALWYWMN V  V+ QGFGATIRA+NGA+ECDG N   VQRRV  YTQYC+
Sbjct: 198 ATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGGNSATVQRRVEYYTQYCN 257

Query: 318 QLGVDPGHNLTC 330
           QLGV PG NLTC
Sbjct: 258 QLGVAPGDNLTC 268

[Close]

BLAST of Spg035822 vs. ExPASy TrEMBL
Match: A0A6J1EGZ6 (endochitinase EP3 OS=Cucurbita moschata OX=3662 GN=LOC111434008 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 3.2e-107
Identity = 182/252 (72.22%), Postives = 205/252 (81.35%), Query Frame = 0


Query: 78  AGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFF 137
           A A  CGC+AGLCCSR GFCG GN +CG GCREGPC   P  +P +N  V+ D VTE FF
Sbjct: 18  AAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPL-TPSVNDVVVADFVTEEFF 77

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLY 197
             I    D  CAG+ FYSR  F+EA++SFD FGRIGSVDDSKREIAAFF HVT+E+G+L 
Sbjct: 78  NGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHLC 137

Query: 198 FVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG T DYCD++ TQYPCNPSK YFGRGPIQLSWNFNYGPAGE+IGFDGLNNPEIV
Sbjct: 138 FIEEIDGATKDYCDEDNTQYPCNPSKGYFGRGPIQLSWNFNYGPAGESIGFDGLNNPEIV 197

Query: 258 ASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCD 317
           A++P+VSFKTALWYWMN V  V+ QGFGATIRA+NGA+ECDGAN   VQRRV  YTQYC+
Sbjct: 198 ATNPLVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANSATVQRRVEYYTQYCN 257

Query: 318 QLGVDPGHNLTC 330
           QLGV PG NLTC
Sbjct: 258 QLGVAPGDNLTC 268

[Close]

BLAST of Spg035822 vs. ExPASy TrEMBL
Match: A0A6J1CFV8 (endochitinase EP3-like OS=Momordica charantia OX=3673 GN=LOC111010839 PE=4 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.6e-106
Identity = 179/252 (71.03%), Postives = 204/252 (80.95%), Query Frame = 0


Query: 78  AGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFF 137
           A A  CGC+AGLCCSR GFCG G  +CG+GCREGPC   P  SP +N  V+ D VTE FF
Sbjct: 24  AAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPL-SPSVNDVVVADFVTEEFF 83

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLY 197
             I    D  CAG+ FYSR  F++A++SFD FGRIGSVDDSKREIAAFF HVT+E+G+  
Sbjct: 84  NGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGHFC 143

Query: 198 FVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEI+G +GDYCD+E TQYPCNPSK Y+GRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV
Sbjct: 144 FIEEINGPSGDYCDEENTQYPCNPSKGYYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 203

Query: 258 ASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCD 317
           A+DPVVSFKTALWYWM+ V  V+ QGFGATIRA+NGA+ECDG N   VQRR+  YTQYC+
Sbjct: 204 ATDPVVSFKTALWYWMSFVRPVINQGFGATIRAINGALECDGGNSATVQRRIQFYTQYCN 263

Query: 318 QLGVDPGHNLTC 330
           QLGV PG NLTC
Sbjct: 264 QLGVAPGDNLTC 274

[Close]

BLAST of Spg035822 vs. ExPASy TrEMBL
Match: A0A5A7UFI0 (Endochitinase EP3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G00900 PE=4 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 3.0e-105
Identity = 178/252 (70.63%), Postives = 202/252 (80.16%), Query Frame = 0


Query: 78  AGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFF 137
           A A  CGC+AGLCCSR GFCG G  +CG GCREGPC   P  +P +N   + + VTE FF
Sbjct: 25  AAAQNCGCAAGLCCSRFGFCGTGEDFCGTGCREGPCNIPPL-TPSVNDVNVAEFVTEEFF 84

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLY 197
             I    D  CAG+ FYSR  F+EA++SFD FGRIG+VDDSKREIAAFF+HVT+E+G+  
Sbjct: 85  NGIINQADASCAGRGFYSRATFLEALQSFDRFGRIGTVDDSKREIAAFFAHVTHETGHFC 144

Query: 198 FVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG T DYCD+E TQYPCNPSK YFGRGPIQLSWNFNYGPAGE IGFDGLNNPEIV
Sbjct: 145 FIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGENIGFDGLNNPEIV 204

Query: 258 ASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCD 317
           A++PVVSFKTALWYWMN V  V+ QGFGATIRA+NGA+ECDGAN   VQRRV  Y QYC+
Sbjct: 205 ATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANTATVQRRVQFYNQYCN 264

Query: 318 QLGVDPGHNLTC 330
           QL VDPG+NLTC
Sbjct: 265 QLNVDPGNNLTC 275

[Close]

BLAST of Spg035822 vs. ExPASy TrEMBL
Match: A0A1S3BAS2 (endochitinase EP3-like OS=Cucumis melo OX=3656 GN=LOC103487878 PE=4 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 3.0e-105
Identity = 178/252 (70.63%), Postives = 202/252 (80.16%), Query Frame = 0


Query: 78  AGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFF 137
           A A  CGC+AGLCCSR GFCG G  +CG GCREGPC   P  +P +N   + + VTE FF
Sbjct: 25  AAAQNCGCAAGLCCSRFGFCGTGEDFCGTGCREGPCNIPPL-TPSVNDVNVAEFVTEEFF 84

Query: 138 ENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLY 197
             I    D  CAG+ FYSR  F+EA++SFD FGRIG+VDDSKREIAAFF+HVT+E+G+  
Sbjct: 85  NGIINQADASCAGRGFYSRATFLEALQSFDRFGRIGTVDDSKREIAAFFAHVTHETGHFC 144

Query: 198 FVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIV 257
           F+EEIDG T DYCD+E TQYPCNPSK YFGRGPIQLSWNFNYGPAGE IGFDGLNNPEIV
Sbjct: 145 FIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGENIGFDGLNNPEIV 204

Query: 258 ASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCD 317
           A++PVVSFKTALWYWMN V  V+ QGFGATIRA+NGA+ECDGAN   VQRRV  Y QYC+
Sbjct: 205 ATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANTATVQRRVQFYNQYCN 264

Query: 318 QLGVDPGHNLTC 330
           QL VDPG+NLTC
Sbjct: 265 QLNVDPGNNLTC 275

[Close]

BLAST of Spg035822 vs. TAIR 10
Match: AT3G54420.1 (homolog of carrot EP3-3 chitinase )

HSP 1 Score: 361.3 bits (926), Expect = 8.2e-100
Identity = 163/250 (65.20%), Postives = 193/250 (77.20%), Query Frame = 0


Query: 80  APKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTEVFFEN 139
           A  CGCS+ LCCS+ GFCG  + YCG GC++GPC A P      NG  + +IVT+ FF  
Sbjct: 28  AQNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFAPPP----ANGVSVAEIVTQEFFNG 87

Query: 140 ITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESGNLYFV 199
           I       CAG RFYSR AF+EA++S+  FGR+GS DDS+REIAAFF+HVT+E+G+  ++
Sbjct: 88  IISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFFAHVTHETGHFCYI 147

Query: 200 EEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVAS 259
           EEIDG + DYCD+  TQYPCNP+K Y+GRGPIQLSWNFNYGPAG AIGFDGLN PE VA+
Sbjct: 148 EEIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVAT 207

Query: 260 DPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQYCDQL 319
           DPV+SFKTALWYW N V  V+ QGFGATIRA+NGA+ECDGAN   VQ RV  YT YC QL
Sbjct: 208 DPVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTATVQARVRYYTDYCRQL 267

Query: 320 GVDPGHNLTC 330
           GVDPG+NLTC
Sbjct: 268 GVDPGNNLTC 273

[Close]

BLAST of Spg035822 vs. TAIR 10
Match: AT2G43590.1 (Chitinase family protein )

HSP 1 Score: 293.5 bits (750), Expect = 2.1e-79
Identity = 132/255 (51.76%), Postives = 172/255 (67.45%), Query Frame = 0


Query: 75  NEIAGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTE 134
           +E   +  CGC+  LCCS+ G+CG  + YCG GCR GPC+ +  P+ G     +G IVT+
Sbjct: 19  SETVKSQNCGCAPNLCCSQFGYCGTDDAYCGVGCRSGPCRGSGTPTGGS----VGSIVTQ 78

Query: 135 VFFENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESG 194
            FF NI       CAGKRFY+R +FV A  +F  F        ++REIA  F+H T+E+G
Sbjct: 79  GFFNNIINQAGNGCAGKRFYTRDSFVNAANTFPNFAN----SVTRREIATMFAHFTHETG 138

Query: 195 NLYFVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNP 254
           +  ++EEI+G T +YC    TQYPC P K YFGRGPIQLSWN+NYG  G+++G D L  P
Sbjct: 139 HFCYIEEINGATRNYCQSSNTQYPCAPGKGYFGRGPIQLSWNYNYGACGQSLGLDLLRQP 198

Query: 255 EIVASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQ 314
           E+V S+P V+F+T LW+WMN+V  V+ QGFGATIRA+NG +EC+G N GAV  R+  Y  
Sbjct: 199 ELVGSNPTVAFRTGLWFWMNSVRPVLNQGFGATIRAING-MECNGGNSGAVNARIGYYRD 258

Query: 315 YCDQLGVDPGHNLTC 330
           YC QLGVDPG NL+C
Sbjct: 259 YCGQLGVDPGPNLSC 264

[Close]

BLAST of Spg035822 vs. TAIR 10
Match: AT2G43580.1 (Chitinase family protein )

HSP 1 Score: 270.4 bits (690), Expect = 1.9e-72
Identity = 121/255 (47.45%), Postives = 168/255 (65.88%), Query Frame = 0


Query: 75  NEIAGAPKCGCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGTVLGDIVTE 134
           +E   +  C C+  LCCS+ G+CG    YCG+ C+ GPC+    P+      ++G+IVT+
Sbjct: 19  SETVKSQNCDCAPNLCCSQFGYCGTTADYCGSTCQSGPCRVGGPPT---GAGLVGNIVTQ 78

Query: 135 VFFENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESG 194
           +FF NI       CAGK FY+R +F+ A  +F  F        ++REIA  F+H TYE+G
Sbjct: 79  IFFNNIINQAGNGCAGKSFYTRDSFINATNTFPSFAN----TVTRREIATMFAHFTYETG 138

Query: 195 NLYFVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNP 254
           +  ++EEI+G +   CD+   QYPC P+K Y GRGP+ LSWNFNYG  G+++G D L  P
Sbjct: 139 HFCYIEEINGASRVMCDQNNRQYPCAPAKSYHGRGPLLLSWNFNYGACGQSLGLDLLRQP 198

Query: 255 EIVASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQ 314
           E+V+S+PVV+F+TALW+WM +V  V+ QGFGATIRA++G  +CDG N G V  R+  Y  
Sbjct: 199 ELVSSNPVVAFRTALWFWMKSVRPVLNQGFGATIRAISG-FDCDGRNLGGVNARIGYYRD 258

Query: 315 YCDQLGVDPGHNLTC 330
           YC QLG+DPG N+TC
Sbjct: 259 YCGQLGLDPGANITC 265

[Close]

BLAST of Spg035822 vs. TAIR 10
Match: AT2G43620.1 (Chitinase family protein )

HSP 1 Score: 240.4 bits (612), Expect = 2.1e-63
Identity = 119/257 (46.30%), Postives = 161/257 (62.65%), Query Frame = 0


Query: 84  GCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAA-----PAPSPGMNG------TVLGDIV 143
           GC+A LCCSR+G+CG  + YCG GCR GPC ++     P PS G  G        + ++V
Sbjct: 35  GCAANLCCSRYGYCGTTDAYCGTGCRSGPCSSSTTPIPPTPSGGAGGLNADPRDTIENVV 94

Query: 144 TEVFFENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYE 203
           T  FF+ I       C  K FY+R+AF+ A +SFD +   G+V  +KREIAA  +  ++E
Sbjct: 95  TPAFFDGIMSKVGNGCPAKGFYTRQAFIAAAQSFDAYK--GTV--AKREIAAMLAQFSHE 154

Query: 204 SGNLYFVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLN 263
           SG+  + EEI    G YC    T YPC P K Y+GRGPIQ++WN+NYG AG+ +G   L 
Sbjct: 155 SGSFCYKEEI--ARGKYC-SPSTAYPCTPGKDYYGRGPIQITWNYNYGAAGKFLGLPLLT 214

Query: 264 NPEIVASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLY 323
           +P++VA  P V+F+ A+W+W  NV  V+ QGFGAT R +NG  EC+G  P AVQ RV  Y
Sbjct: 215 DPDMVARSPQVAFQCAMWFWNLNVRPVLDQGFGATTRKINGG-ECNGRRPAAVQSRVNYY 274

Query: 324 TQYCDQLGVDPGHNLTC 330
            ++C  LG+ PG NL+C
Sbjct: 275 LEFCRTLGITPGANLSC 283

[Close]

BLAST of Spg035822 vs. TAIR 10
Match: AT2G43610.1 (Chitinase family protein )

HSP 1 Score: 238.8 bits (608), Expect = 6.2e-63
Identity = 115/255 (45.10%), Postives = 156/255 (61.18%), Query Frame = 0


Query: 84  GCSAGLCCSRHGFCGVGNGYCGAGCREGPCKAAPAPSPGMNGT---------VLGDIVTE 143
           GC   +CCSR G+CG    YCG GC+ GPC + P P+P  +G+          +  ++T 
Sbjct: 35  GCKGNMCCSRWGYCGTTKAYCGTGCQSGPCNSKPKPTPTPSGSGGLNAGPRGTIASVITP 94

Query: 144 VFFENITGGGDERCAGKRFYSRKAFVEAVESFDEFGRIGSVDDSKREIAAFFSHVTYESG 203
            FF +I       C  K FY+R+AF+ A ESF  +   G+V  +KREIAA  +  ++ESG
Sbjct: 95  AFFNSIMSKVGSGCPAKGFYTRQAFIAAAESFAAYK--GTV--AKREIAAMLAQFSHESG 154

Query: 204 NLYFVEEIDGETGDYCDKEKTQYPCNPSKRYFGRGPIQLSWNFNYGPAGEAIGFDGLNNP 263
           +  + EEI    G YC    T YPC P K Y+GRGPIQ++WN+NYG AG+ +G   L +P
Sbjct: 155 SFCYKEEI--ARGRYC-SPSTTYPCQPGKNYYGRGPIQITWNYNYGAAGKFLGLPLLKDP 214

Query: 264 EIVASDPVVSFKTALWYWMNNVHSVVGQGFGATIRAVNGAVECDGANPGAVQRRVMLYTQ 323
           ++VA  P V+F+ A+W+W  NV  V+ QGFGAT R +NG  EC+G  P AVQ RV  Y  
Sbjct: 215 DMVARSPTVAFQCAMWFWNKNVRPVLSQGFGATTRRINGG-ECNGGRPAAVQSRVNHYLD 274

Query: 324 YCDQLGVDPGHNLTC 330
           +C +LGV PG NL+C
Sbjct: 275 FCKKLGVTPGTNLSC 281

The following BLAST results are available for this feature:

BLAST of Spg035822 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038894874.1	1.3e-107	73.41	endochitinase EP3-like [Benincasa hispida]	[more]
KAG7019501.1	3.8e-107	72.22	Endochitinase EP3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023001456.1	3.8e-107	72.62	endochitinase EP3 [Cucurbita maxima]	[more]
XP_022927074.1	6.5e-107	72.22	endochitinase EP3 [Cucurbita moschata]	[more]
KAG6583882.1	6.5e-107	72.22	Endochitinase EP3, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Spg035822 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2U5	1.2e-98	65.20	Endochitinase EP3 OS=Arabidopsis thaliana OX=3702 GN=EP3 PE=1 SV=1	[more]
P27054	9.8e-90	61.51	Endochitinase PR4 OS=Phaseolus vulgaris OX=3885 GN=CHI4 PE=2 SV=1	[more]
Q7Y1Z0	4.6e-87	54.96	Chitinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht5 PE=2 SV=1	[more]
Q6K8R2	6.0e-87	55.13	Chitinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht6 PE=2 SV=1	[more]
O04138	2.5e-85	55.17	Chitinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht4 PE=2 SV=2	[more]

BLAST of Spg035822 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KIN5	1.8e-107	72.62	endochitinase EP3 OS=Cucurbita maxima OX=3661 GN=LOC111495584 PE=4 SV=1	[more]
A0A6J1EGZ6	3.2e-107	72.22	endochitinase EP3 OS=Cucurbita moschata OX=3662 GN=LOC111434008 PE=4 SV=1	[more]
A0A6J1CFV8	1.6e-106	71.03	endochitinase EP3-like OS=Momordica charantia OX=3673 GN=LOC111010839 PE=4 SV=1	[more]
A0A5A7UFI0	3.0e-105	70.63	Endochitinase EP3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4...	[more]
A0A1S3BAS2	3.0e-105	70.63	endochitinase EP3-like OS=Cucumis melo OX=3656 GN=LOC103487878 PE=4 SV=1	[more]

BLAST of Spg035822 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G54420.1	8.2e-100	65.20	homolog of carrot EP3-3 chitinase	[more]
AT2G43590.1	2.1e-79	51.76	Chitinase family protein	[more]
AT2G43580.1	1.9e-72	47.45	Chitinase family protein	[more]
AT2G43620.1	2.1e-63	46.30	Chitinase family protein	[more]
AT2G43610.1	6.2e-63	45.10	Chitinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001002	Chitin-binding, type 1	SMART	SM00270	ChitinBD_3	coord: 32..61 e-value: 1.2 score: 10.9 coord: 82..113 e-value: 1.9E-7 score: 40.7
IPR001002	Chitin-binding, type 1	PROSITE	PS50941	CHIT_BIND_I_2	coord: 80..115 score: 8.941624
IPR000726	Glycoside hydrolase, family 19, catalytic	PFAM	PF00182	Glyco_hydro_19	coord: 282..329 e-value: 4.3E-7 score: 29.9 coord: 132..275 e-value: 5.2E-41 score: 140.9
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00773	CHITINASE_19_1	coord: 148..170
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00774	CHITINASE_19_2	coord: 263..273
IPR016283	Glycoside hydrolase, family 19	PIRSF	PIRSF001060	Endochitinase	coord: 7..76 e-value: 0.012 score: 13.0 coord: 62..329 e-value: 5.6E-93 score: 309.6
None	No IPR available	GENE3D	1.10.530.10		coord: 127..329 e-value: 5.2E-52 score: 177.8
None	No IPR available	GENE3D	3.30.20.10	Endochitinase, domain 2	coord: 200..257 e-value: 1.1E-26 score: 94.4
None	No IPR available	PANTHER	PTHR22595:SF131	ENDOCHITINASE EP3	coord: 74..329
None	No IPR available	PANTHER	PTHR22595	CHITINASE-RELATED	coord: 74..329
None	No IPR available	PANTHER	PTHR22595	CHITINASE-RELATED	coord: 18..61
None	No IPR available	PANTHER	PTHR22595:SF131	ENDOCHITINASE EP3	coord: 18..61
None	No IPR available	CDD	cd00035	ChtBD1	coord: 85..108 e-value: 1.56868E-5 score: 39.6762
None	No IPR available	CDD	cd00325	chitinase_GH19	coord: 132..329 e-value: 7.49363E-80 score: 240.795
IPR036861	Endochitinase-like superfamily	GENE3D	3.30.60.10		coord: 77..126 e-value: 5.9E-8 score: 34.7
IPR036861	Endochitinase-like superfamily	SUPERFAMILY	57016	Plant lectins/antimicrobial peptides	coord: 33..66
IPR036861	Endochitinase-like superfamily	SUPERFAMILY	57016	Plant lectins/antimicrobial peptides	coord: 80..116
IPR018371	Chitin-binding, type 1, conserved site	PROSITE	PS00026	CHIT_BIND_I_1	coord: 85..104
IPR023346	Lysozyme-like domain superfamily	SUPERFAMILY	53955	Lysozyme-like	coord: 129..329

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg035822.1	Spg035822.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0016998	cell wall macromolecule catabolic process
biological_process	GO:0006032	chitin catabolic process
biological_process	GO:0006952	defense response
molecular_function	GO:0004568	chitinase activity
molecular_function	GO:0008061	chitin binding