Spg035763 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg035763 vs. NCBI nr
Match: XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 843.2 bits (2177), Expect = 1.2e-240
Identity = 411/533 (77.11%), Postives = 463/533 (86.87%), Query Frame = 0

Query: 7   ISLALAFIVLSSSSSWVTASTNKHQYFLQCLSDHSSSSS-SISKVVYTPINSSYSSVLNF 66
           +S +L FI LSSSS W  AS +KH+ FL CL DHS  S+ SISKVVYTP+NSSYSSVL+F
Sbjct: 10  LSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSYSSVLDF 69

Query: 67  SIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAHLP 126
           SIRNLRFSTA TPKPL IITPLH+SH+QAA++CSR H LQIRTRSGGHDFEGLS V+HLP
Sbjct: 70  SIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLSCVSHLP 129

Query: 127 FIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGHFS 186
           FIIIDLINLRSI +D  NNTAWVQSGATLGELYY IAEKSRTLAFPAG CPTVG+GGHFS
Sbjct: 130 FIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVGIGGHFS 189

Query: 187 GGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 246
           GGGYGL+LRK+GLAADNVIDAYLVDANGKF DRE MGEDLFWAIRGGGGGSFGIVVAWKV
Sbjct: 190 GGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGIVVAWKV 249

Query: 247 KLVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVY-----HGGNISTQGG-KT 306
           KLV VPATVT CYYNRTLEE GAI+L+HRWQYV NKLD+N++      GGN + QGG KT
Sbjct: 250 KLVPVPATVTSCYYNRTLEE-GAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQGGDKT 309

Query: 307 SPRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAGGFQIGEPLEALL 366
           +P A+FFSLFLGK DE  AIL +TFPELGL ++ C+E+SWIESAL++ GF+IG+ LEALL
Sbjct: 310 NPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSAGFKIGDSLEALL 369

Query: 367 NRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESET 426
           NRT  T+GSIKIKSDYVKEPIS+A+IEGIWERLKS+D+ + Q++FVPYGGRM +ISESE 
Sbjct: 370 NRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGGRMSQISESEI 429

Query: 427 PFSHRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLDI 486
           PFSHR GNLYKIGY + W++  +D EKRHL WIR +Y YM PFVSKSPRSAYVNYRDLDI
Sbjct: 430 PFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYVNYRDLDI 489

Query: 487 GSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPPLIP 533
           GSNNKYGKT +K+ASVWG KYFGNNFNRLV VK+KVDPY+ F HEQSIP + P
Sbjct: 490 GSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTISP 541

BLAST of Spg035763 vs. NCBI nr
Match: XP_022142587.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 822.4 bits (2123), Expect = 2.3e-234
Identity = 407/534 (76.22%), Postives = 463/534 (86.70%), Query Frame = 0

Query: 6   LISLALAFIVLSSSSSWVTASTNKHQYFLQCLSDHS--SSSSSISKVVYTPINSSYSSVL 65
           LISL+LAFI LSSSS W +A  NKH+ FLQCL DHS   S+ SISKV++TPINSSYSSVL
Sbjct: 7   LISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSSVL 66

Query: 66  NFSIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAH 125
           +FSIRNLRFST +TPKPL+I+TP H+SHIQAA+ICS+++  QIRTRSGGHDFEGLSYVAH
Sbjct: 67  DFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYVAH 126

Query: 126 LPFIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGH 185
           LPFII+DLINLRSI +D+++NTAWVQSGATLGELYY IA+KSRTLAFPAG+CPTVGVGGH
Sbjct: 127 LPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVGGH 186

Query: 186 FSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAW 245
           FSGGGYGLLLRKYGLAADNVIDAYLVDA+GKFH+RESMGEDLFWAIRGGGGGSFGIVVAW
Sbjct: 187 FSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAW 246

Query: 246 KVKLVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVY-----HGGNISTQ-GG 305
           KVKLV VPATVT+C  NRTLEE GA+++ HRWQYV NKLD+N++      GG I++Q GG
Sbjct: 247 KVKLVPVPATVTLCSANRTLEE-GAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGG 306

Query: 306 KTSPRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAG-GFQIGEPLE 365
           KT+P A FFSLFLGK DEL++IL + FPEL L +K CIETSWIES ++ G GF     LE
Sbjct: 307 KTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTLE 366

Query: 366 ALLNRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISE 425
           ALL+RT  T+ S KIKSDYVKEP+S+A ++GIW+RL SQDIE   +IFVPYGGRM +ISE
Sbjct: 367 ALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQISE 426

Query: 426 SETPFSHRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRD 485
           SETPFSHR G LYKIGY  GW+E G+DAEKRHL WIR +YDYM PFVSKSPR+AY NYRD
Sbjct: 427 SETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANYRD 486

Query: 486 LDIGSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPPL 531
           LDIGSNNK+GKT YKQASVWGLKYFGNNFNRLV VK+KVDPY+ F HEQSIP L
Sbjct: 487 LDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of Spg035763 vs. NCBI nr
Match: XP_022152518.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 815.5 bits (2105), Expect = 2.8e-232
Identity = 404/538 (75.09%), Postives = 456/538 (84.76%), Query Frame = 0

Query: 1   MKTFSLISLALAFIVLSSSSSWVTASTNKHQYFLQCLSDHSSSSSSISKVVYTPINSSYS 60
           MK   L  L+LAFI+LSSSSSW  AS  KH+ FLQCLS HS+ SSSISKV+YTPIN SYS
Sbjct: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60

Query: 61  SVLNFSIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSY 120
           SVLNFS+RNLRFS+A+TPKPL+IITP ++SH+QAA+ICS+ H LQIR RSGGHD EGLSY
Sbjct: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120

Query: 121 VAHLPFIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGV 180
           VAHLPFIIIDLINL+SI V++EN+TAW+QSGATLGELYY IA+KSRTLAFPAGVCPTVGV
Sbjct: 121 VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV 180

Query: 181 GGHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIV 240
           GGHFSGGGYGL+LRKYGLAADNVIDAYL+DANGKF+DRESMGEDLFWAIRGGGGGSFGIV
Sbjct: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240

Query: 241 VAWKVKLVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVYH-----GGNISTQ 300
           VAWKVKLV VPA VT C  +R L+ED  I L+HRWQ V  KLD+N+Y      GGN ST+
Sbjct: 241 VAWKVKLVPVPAIVTTCSSDRNLDED-TINLIHRWQSVAYKLDENLYFGIILAGGNASTE 300

Query: 301 GG--KTSPRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAG-GFQIG 360
           GG  K +P A FFSLFLGK DEL+AIL + FPEL L +K C+ETSWIES L+A    Q  
Sbjct: 301 GGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTI 360

Query: 361 EPLEALLNRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMG 420
           E LE+LLNRT  T  + K+KSDYVKEPI +A+IEGIWERLK+QDIE  Q++FVPYGGRM 
Sbjct: 361 ESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMS 420

Query: 421 RISESETPFSHRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYV 480
           RIS+SETPFSHR  NLYKIGY +GWKE  + AE+RHLSWIR +YDYM PFVSKSPR+ YV
Sbjct: 421 RISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYV 480

Query: 481 NYRDLDIGSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPPL 531
           NYRDLDIG+NNKYGKT YKQASVWG KYFGNNFNRLV +KSKVDP N F +EQSIPPL
Sbjct: 481 NYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537

BLAST of Spg035763 vs. NCBI nr
Match: KAG6583829.1 (Berberine bridge enzyme-like 26, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 810.8 bits (2093), Expect = 6.8e-231
Identity = 397/529 (75.05%), Postives = 455/529 (86.01%), Query Frame = 0

Query: 7   ISLALAF-IVLSSSSSWVTASTNKHQYFLQCLSDHSSSSSSISKVVYTPINSSYSSVLNF 66
           I L L F IVLSSS  W + S + HQ+FL CLS HS  +  ISK+VYTPINSSYS VLNF
Sbjct: 4   IPLTLIFIIVLSSSPLWASPSLHIHQHFLHCLSHHSPHTYPISKLVYTPINSSYSYVLNF 63

Query: 67  SIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAHLP 126
           SIRNL FS  +TPKPL+IITP HISHIQAA+ICS+ HDLQIRTRSGGHDFEGLSYVA  P
Sbjct: 64  SIRNLIFSNPQTPKPLLIITPSHISHIQAAVICSKAHDLQIRTRSGGHDFEGLSYVADQP 123

Query: 127 FIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGHFS 186
           FII+DLINLRSI+VDV+ NTAWVQSGATLGELYYRI EKSR+LAFPAG+CPTVGVGGHFS
Sbjct: 124 FIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGEKSRSLAFPAGICPTVGVGGHFS 183

Query: 187 GGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 246
           GGGYGL+LRKYGLAADNV+DAYLVDANG+ HDRESMGEDLFWAIRGGGGGSFGIVVAWKV
Sbjct: 184 GGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 243

Query: 247 KLVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVY-----HGGNISTQGGKTS 306
           KLV VPA VT+C  NR +E+D AI+L+HRWQYV N+LDD+++      GGN ST+ G T+
Sbjct: 244 KLVVVPAVVTMCSTNRDVEDD-AIKLIHRWQYVANQLDDDLFLGILLTGGNFSTREGITN 303

Query: 307 PRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAG-GFQIGEPLEALL 366
           P A FFSL+LGK+DEL+AILK+TFPELGLT+K C+E+SWI+S LIA  G Q  EPLEALL
Sbjct: 304 PTATFFSLYLGKLDELIAILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALL 363

Query: 367 NRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESET 426
           NRT  T  S K KSDYVK+PI +A+IEGIW+RLK+QDIE  Q++F+PYGGRM +ISESET
Sbjct: 364 NRTPPTIESTKFKSDYVKDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESET 423

Query: 427 PFSHRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLDI 486
           PFSHR GNLYKIGY + WKE  +DA+ RH++WIR VY+YMAPFVSKSPR+AYVNYRDLDI
Sbjct: 424 PFSHRAGNLYKIGYAVSWKEQSLDAKNRHINWIREVYEYMAPFVSKSPRAAYVNYRDLDI 483

Query: 487 GSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIP 529
           G+NNKYGKT Y+QASVWG+KYFGNNFNRLV VK+ VDP++ F HEQSIP
Sbjct: 484 GTNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTMVDPHDFFRHEQSIP 531

BLAST of Spg035763 vs. NCBI nr
Match: XP_023520318.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 810.4 bits (2092), Expect = 8.9e-231
Identity = 397/529 (75.05%), Postives = 455/529 (86.01%), Query Frame = 0

Query: 7   ISLALAF-IVLSSSSSWVTASTNKHQYFLQCLSDHSSSSSSISKVVYTPINSSYSSVLNF 66
           I L L F IVLSSS  W + S + HQ+FL CLS HS  +  ISK+VYTPINSSYSSVLNF
Sbjct: 4   IPLTLIFIIVLSSSPLWASPSLHIHQHFLHCLSHHSPHTYPISKLVYTPINSSYSSVLNF 63

Query: 67  SIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAHLP 126
           SIRNL FS  +TPKPL+IITP HISHIQAA+ICS+ HDLQIRTRSGGHDFEGLSYVA  P
Sbjct: 64  SIRNLIFSNPQTPKPLLIITPSHISHIQAAVICSKAHDLQIRTRSGGHDFEGLSYVADQP 123

Query: 127 FIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGHFS 186
           FII+DLINLRSI+VDV+ NTAWVQSGATLGELYYRI EKSR+LAFPAG+CPTVGVGGHFS
Sbjct: 124 FIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGEKSRSLAFPAGICPTVGVGGHFS 183

Query: 187 GGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 246
           GGGYGL+LRKYGLAADNV+DAYLVDANG+ HDRESMGEDLFWAIRGGGGGSFGIVVAWKV
Sbjct: 184 GGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 243

Query: 247 KLVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVY-----HGGNISTQGGKTS 306
           KLV VPA VT+C  NR +E+D AI+L+HRWQYV N+LDDN++      GGN ST+ G T+
Sbjct: 244 KLVVVPAVVTMCSTNRDVEDD-AIKLIHRWQYVANQLDDNLFLGILLTGGNFSTRQGITN 303

Query: 307 PRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAG-GFQIGEPLEALL 366
           P A FFSL+LGK+DEL+AILK+TFPELGLT+K C+E+SWI+S LIA  G Q  EPLEALL
Sbjct: 304 PTATFFSLYLGKLDELIAILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALL 363

Query: 367 NRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESET 426
           NRT  T  S K KSDYVK+PI +A+IEGIW+RLK+QDIE  Q++F+PYGGRM +ISESET
Sbjct: 364 NRTPPTIESTKFKSDYVKDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESET 423

Query: 427 PFSHRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLDI 486
           PFSHR GNLYKIGY + WKE  +DA+ RH++WIR V ++M PFVSKSPR+AYVNYRDLDI
Sbjct: 424 PFSHRAGNLYKIGYAVSWKEQSLDAKNRHINWIREVNEHMTPFVSKSPRAAYVNYRDLDI 483

Query: 487 GSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIP 529
           G+NNKYGKT Y+QASVWG+KYFGNNFNRLV VK+KVDP++ F HEQSIP
Sbjct: 484 GTNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

BLAST of Spg035763 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 2.1e-150
Identity = 272/535 (50.84%), Postives = 373/535 (69.72%), Query Frame = 0

Query: 1   MKTFSLISLALAFI----VLSSSSSWVTASTNKHQYFLQCLSDHSSSSSSISKVVYTPIN 60
           MK  S  S  L F     +L S    V+A+ +    FLQCLS   + S+ +SKV++TP +
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPND 60

Query: 61  SSYSSVLNFSIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFE 120
           +S+SSVL  SI+N RFS    PKP++I+TP+  S +Q+A+ C+R   + IRTRSGGHD+E
Sbjct: 61  TSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYE 120

Query: 121 GLSYVAHLPFIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCP 180
           GLSYV H PF+I+DL NLRSI VDV+N + WVQ+GAT+GELYY I +K+RTLAFPAGVCP
Sbjct: 121 GLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCP 180

Query: 181 TVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGS 240
           TVGVGGHFSGGGYG LLRK+GLAAD+VIDA +VDA G+  +R  MGED FWAIRGGGG S
Sbjct: 181 TVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSS 240

Query: 241 FGIVVAWKVKLVAVPATVTVCYYNRT-LEEDGAIELVHRWQYVGNKLDDNVYHGGNISTQ 300
           F +V++WK+ L+ VP+TVTV  +N T   E  A++++HRWQ+V +K+ D+++    +  Q
Sbjct: 241 FCVVLSWKIGLINVPSTVTV--FNVTKFSEQSALKIIHRWQFVADKVSDDLFI--RVMLQ 300

Query: 301 GGKTSPRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAGGFQIG-EP 360
             K   RA+F  L+LG V  L+ ++   FPELGL E  C E SWIES +     ++G EP
Sbjct: 301 RYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA--ELGEEP 360

Query: 361 LEALLNRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRI 420
           +  L  RT +   + K KSD+V+EP+ + +I  +W RL+  + E +Q+IF P+GG+M  I
Sbjct: 361 INVLTKRT-RASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEI 420

Query: 421 SESETPFSHRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNY 480
           ++ ETPF HR GN+Y+I Y   W+    D +++++ W+  VYD M+ FV+KSPR AY+N 
Sbjct: 421 ADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINL 480

Query: 481 RDLDIGSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPP 530
           RDLD+G      +++Y++   WG+KYF NNF RLV+VK+ VDP + F  EQSIPP
Sbjct: 481 RDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of Spg035763 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 256/516 (49.61%), Postives = 353/516 (68.41%), Query Frame = 0

Query: 28  NKHQYFLQCLSDHSSSSSSISKVVYTPINSSYSSVLNFSIRNLRFSTAKTPKPLIIITPL 87
           N  + FL+C S H  ++ +  K+VYT  +  Y S+LN +I+NLRF +  TPKPL+I+TP 
Sbjct: 29  NPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPS 88

Query: 88  HISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAHLPFIIIDLINLRSIDVDVENNTAW 147
           + SHIQA I+CS+   LQIRTRSGGHD EG+SY++ +PF+++DL N+ SI +DV + TAW
Sbjct: 89  NNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAW 148

Query: 148 VQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGGGYGLLLRKYGLAADNVIDAY 207
           V++GATLGE+YY I EK+  L+FP G CPTVGVGGHFSGGGYG L+R YGLAADN+IDA+
Sbjct: 149 VEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAH 208

Query: 208 LVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVAVPATVTVCYYNRTLEEDG 267
           LV+ +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLVAVP+  T+    + +E  G
Sbjct: 209 LVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHG 268

Query: 268 AIELVHRWQYVGNKLDDNV-----YHGGNISTQGG--KTSPRAAFFSLFLGKVDELVAIL 327
            ++L ++WQ +  K D ++     +   NI+   G  KT+    F S+F G VD LV ++
Sbjct: 269 LVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLM 328

Query: 328 KSTFPELGLTEKACIETSWIESALIAGGF----QIGEPLEALLNRTLQTHGSIKIKSDYV 387
             +FPELG+ +  C E SWI++ +   G           E LL+R+     +  IK DYV
Sbjct: 329 NKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYV 388

Query: 388 KEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESETPFSHRVGNLYKIGYTIG 447
           K+PI + ++  I E+L  +D+ +   +  PYGG M  ISES  PF HR G +Y++ YT  
Sbjct: 389 KKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTAS 448

Query: 448 WKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLDIGSNNKYGKTRYKQASVW 507
           W++   +  ++H++W+R VY++  P+VS++PR AY+NYRDLD+G  N      Y QA +W
Sbjct: 449 WEK--QEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIW 508

Query: 508 GLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPPLIP 533
           G KYFG NFNRLV+VK+KVDP N F +EQSIPPL P
Sbjct: 509 GEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPP 542

BLAST of Spg035763 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 8.3e-147
Identity = 255/513 (49.71%), Postives = 362/513 (70.57%), Query Frame = 0

Query: 20  SSWVTASTNKHQYFLQCLSDHSSSSSSISKVVYTPINSSYSSVLNFSIRNLRFSTAKTPK 79
           S  V+A  + H+ FL+CLS   + ++   KV++T  +SS+ S+L+ SI+N RFS ++TPK
Sbjct: 18  SPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPK 77

Query: 80  PLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAH-LPFIIIDLINLRSID 139
           P+ IITP+  S +Q  I C++ H + +RTRS GH +EGLSY+A+  PF +IDL NLRSI 
Sbjct: 78  PVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSIS 137

Query: 140 VDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGGGYGLLLRKYGL 199
           +DV+N T WVQ+GAT GELYY I + +++LAFPAG+ PTVGVGG FSGGGYG LLRKYGL
Sbjct: 138 LDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGL 197

Query: 200 AADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVAVPATVTVCY 259
           AADN+IDA +VDA+G+  DR++MGED FWAIRGGGG SFG++++WKVKLV VP+T+TV  
Sbjct: 198 AADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFK 257

Query: 260 YNRTLEEDGAIELVHRWQYVGNKLDDNVYHGGNISTQGGKTSPRAAFFSLFLGKVDELVA 319
             +T +++ A+ ++ +WQY  +K+ D+++    +  +  K +  A F  L++G V+ L+A
Sbjct: 258 VQKTSKKE-AVRIIKKWQYAADKVPDDLFIRTTLE-RSNKNAVHALFTGLYIGPVNNLLA 317

Query: 320 ILKSTFPELGLTEKACIETSWIESALIAGGFQIGEPLEALLNRTLQTHGSIKIKSDYVKE 379
           +++  FPELGL ++ C E SWIES L    F  GE L  L NR  +T  S K K D+V+E
Sbjct: 318 LMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLTNRE-RTSLSFKGKDDFVQE 377

Query: 380 PISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESETPFSHRVGNLYKIGYTIGWK 439
           PI +A+I+ IW RL++ +    ++I  P+GG+M  ++E ETPF HR GNLY+I Y   W+
Sbjct: 378 PIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWR 437

Query: 440 E---PGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLDIGSNNKYGKTRYKQASV 499
           E          ++L W+  VY++M P+VSKSPR AYVN++D+D+G      KT+Y++   
Sbjct: 438 EEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKKTKYEEGKS 497

Query: 500 WGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIP 529
           WG+KYF NNF RLV+VK++VDP + F  EQSIP
Sbjct: 498 WGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Spg035763 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 5.4e-146
Identity = 250/516 (48.45%), Postives = 350/516 (67.83%), Query Frame = 0

Query: 28  NKHQYFLQCLSDHSSSSSSISKVVYTPINSSYSSVLNFSIRNLRFSTAKTPKPLIIITPL 87
           N  + FL+C S++  ++ +  K +YT  +  Y SVLN +I+NLRF++  TPKPL+I+TP 
Sbjct: 29  NPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPS 88

Query: 88  HISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAHLPFIIIDLINLRSIDVDVENNTAW 147
           ++SHIQA+I+CS+   LQIRTRSGGHD EGLSY++ +PF I+DL N+ ++ VD+ + TAW
Sbjct: 89  NVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAW 148

Query: 148 VQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGGGYGLLLRKYGLAADNVIDAY 207
           V++GATLGE+YY I E +   +FP G CPTVGVGGHFSGGGYG L+R YGLAADN+IDA+
Sbjct: 149 VEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAH 208

Query: 208 LVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVAVPATVTVCYYNRTLEEDG 267
           LV+ +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T+    + +E  G
Sbjct: 209 LVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHG 268

Query: 268 AIELVHRWQYVGNKLDDNV-----YHGGNISTQGG--KTSPRAAFFSLFLGKVDELVAIL 327
            ++L ++WQ +  K D ++     +   NI+   G  KT+    F S+FLG VD LV ++
Sbjct: 269 LVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLM 328

Query: 328 KSTFPELGLTEKACIETSWIESALIAGGF----QIGEPLEALLNRTLQTHGSIKIKSDYV 387
             +FPELG+ +  C E SWI++ +   G           E LL+R+     +  IK DYV
Sbjct: 329 NKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYV 388

Query: 388 KEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESETPFSHRVGNLYKIGYTIG 447
           K+ I + ++  I E+L  +++     +  PYGG M  ISES  PF HR G +Y++ YT  
Sbjct: 389 KKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTAT 448

Query: 448 WKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLDIGSNNKYGKTRYKQASVW 507
           W++   +  ++H++W+R VY++  P+VS++PR AY+NYRDLD+G  N      Y QA +W
Sbjct: 449 WEK--QEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQARIW 508

Query: 508 GLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPPLIP 533
           G KYFG NFNRLV+VK+K DP N F +EQSIPPL P
Sbjct: 509 GEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLPP 542

BLAST of Spg035763 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 7.0e-146
Identity = 252/514 (49.03%), Postives = 352/514 (68.48%), Query Frame = 0

Query: 28  NKHQYFLQCLSDHSSSSSSISKVVYTPINSSYSSVLNFSIRNLRFSTAKTPKPLIIITPL 87
           N  + FL+C S +  ++ + +K+VYT  +  Y S+LN +I+NLRF++  TPKPL+IITPL
Sbjct: 29  NPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPL 88

Query: 88  HISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAHLPFIIIDLINLRSIDVDVENNTAW 147
           ++SHIQ  I+CS+   LQIRTRSGGHD EG+SY++ +PF+I+DL N+ S+ +DV + TAW
Sbjct: 89  NVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAW 148

Query: 148 VQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGGGYGLLLRKYGLAADNVIDAY 207
           V++GATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG L+R YGLAADN+IDA+
Sbjct: 149 VEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAH 208

Query: 208 LVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVAVPATVTVCYYNRTLEEDG 267
           LV+ +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LVAVP+  T+    + +E   
Sbjct: 209 LVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHE 268

Query: 268 AIELVHRWQYVGNKLDDNV-----YHGGNISTQGG--KTSPRAAFFSLFLGKVDELVAIL 327
            ++LV++WQ +    +  +     +   NI+   G  KT+  + F S+F G VD LV ++
Sbjct: 269 LVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLM 328

Query: 328 KSTFPELGLTEKACIETSWIESALIAGGFQIGEPL----EALLNRTLQTHGSIKIKSDYV 387
             +FPELG+ +  C + SWI++ +   G           E LL+R+     +  IK DYV
Sbjct: 329 NKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYV 388

Query: 388 KEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESETPFSHRVGNLYKIGYTIG 447
           K+PI + ++  I E+L  +D+     +F PYGG M  ISES  PF HR G +Y+I Y   
Sbjct: 389 KKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIAS 448

Query: 448 WKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLDIGSNNKYGKTRYKQASVW 507
           W++   +  ++H++WIR VY++  P+VS++PR AY+NYRDLD+G  N      Y QA +W
Sbjct: 449 WEK--QEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIW 508

Query: 508 GLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPPL 531
           G KYFG NFNRLV+VK+KVDP N F +EQSIPPL
Sbjct: 509 GEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Spg035763 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 6.0e-241
Identity = 411/533 (77.11%), Postives = 463/533 (86.87%), Query Frame = 0

Query: 7   ISLALAFIVLSSSSSWVTASTNKHQYFLQCLSDHSSSSS-SISKVVYTPINSSYSSVLNF 66
           +S +L FI LSSSS W  AS +KH+ FL CL DHS  S+ SISKVVYTP+NSSYSSVL+F
Sbjct: 10  LSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSYSSVLDF 69

Query: 67  SIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAHLP 126
           SIRNLRFSTA TPKPL IITPLH+SH+QAA++CSR H LQIRTRSGGHDFEGLS V+HLP
Sbjct: 70  SIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLSCVSHLP 129

Query: 127 FIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGHFS 186
           FIIIDLINLRSI +D  NNTAWVQSGATLGELYY IAEKSRTLAFPAG CPTVG+GGHFS
Sbjct: 130 FIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVGIGGHFS 189

Query: 187 GGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 246
           GGGYGL+LRK+GLAADNVIDAYLVDANGKF DRE MGEDLFWAIRGGGGGSFGIVVAWKV
Sbjct: 190 GGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGIVVAWKV 249

Query: 247 KLVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVY-----HGGNISTQGG-KT 306
           KLV VPATVT CYYNRTLEE GAI+L+HRWQYV NKLD+N++      GGN + QGG KT
Sbjct: 250 KLVPVPATVTSCYYNRTLEE-GAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQGGDKT 309

Query: 307 SPRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAGGFQIGEPLEALL 366
           +P A+FFSLFLGK DE  AIL +TFPELGL ++ C+E+SWIESAL++ GF+IG+ LEALL
Sbjct: 310 NPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSAGFKIGDSLEALL 369

Query: 367 NRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESET 426
           NRT  T+GSIKIKSDYVKEPIS+A+IEGIWERLKS+D+ + Q++FVPYGGRM +ISESE 
Sbjct: 370 NRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGGRMSQISESEI 429

Query: 427 PFSHRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLDI 486
           PFSHR GNLYKIGY + W++  +D EKRHL WIR +Y YM PFVSKSPRSAYVNYRDLDI
Sbjct: 430 PFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYVNYRDLDI 489

Query: 487 GSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPPLIP 533
           GSNNKYGKT +K+ASVWG KYFGNNFNRLV VK+KVDPY+ F HEQSIP + P
Sbjct: 490 GSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTISP 541

BLAST of Spg035763 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 1.1e-234
Identity = 407/534 (76.22%), Postives = 463/534 (86.70%), Query Frame = 0

Query: 6   LISLALAFIVLSSSSSWVTASTNKHQYFLQCLSDHS--SSSSSISKVVYTPINSSYSSVL 65
           LISL+LAFI LSSSS W +A  NKH+ FLQCL DHS   S+ SISKV++TPINSSYSSVL
Sbjct: 7   LISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSSVL 66

Query: 66  NFSIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAH 125
           +FSIRNLRFST +TPKPL+I+TP H+SHIQAA+ICS+++  QIRTRSGGHDFEGLSYVAH
Sbjct: 67  DFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYVAH 126

Query: 126 LPFIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGH 185
           LPFII+DLINLRSI +D+++NTAWVQSGATLGELYY IA+KSRTLAFPAG+CPTVGVGGH
Sbjct: 127 LPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVGGH 186

Query: 186 FSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAW 245
           FSGGGYGLLLRKYGLAADNVIDAYLVDA+GKFH+RESMGEDLFWAIRGGGGGSFGIVVAW
Sbjct: 187 FSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAW 246

Query: 246 KVKLVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVY-----HGGNISTQ-GG 305
           KVKLV VPATVT+C  NRTLEE GA+++ HRWQYV NKLD+N++      GG I++Q GG
Sbjct: 247 KVKLVPVPATVTLCSANRTLEE-GAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGG 306

Query: 306 KTSPRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAG-GFQIGEPLE 365
           KT+P A FFSLFLGK DEL++IL + FPEL L +K CIETSWIES ++ G GF     LE
Sbjct: 307 KTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTLE 366

Query: 366 ALLNRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISE 425
           ALL+RT  T+ S KIKSDYVKEP+S+A ++GIW+RL SQDIE   +IFVPYGGRM +ISE
Sbjct: 367 ALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQISE 426

Query: 426 SETPFSHRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRD 485
           SETPFSHR G LYKIGY  GW+E G+DAEKRHL WIR +YDYM PFVSKSPR+AY NYRD
Sbjct: 427 SETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANYRD 486

Query: 486 LDIGSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPPL 531
           LDIGSNNK+GKT YKQASVWGLKYFGNNFNRLV VK+KVDPY+ F HEQSIP L
Sbjct: 487 LDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of Spg035763 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.3e-232
Identity = 404/538 (75.09%), Postives = 456/538 (84.76%), Query Frame = 0

Query: 1   MKTFSLISLALAFIVLSSSSSWVTASTNKHQYFLQCLSDHSSSSSSISKVVYTPINSSYS 60
           MK   L  L+LAFI+LSSSSSW  AS  KH+ FLQCLS HS+ SSSISKV+YTPIN SYS
Sbjct: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60

Query: 61  SVLNFSIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSY 120
           SVLNFS+RNLRFS+A+TPKPL+IITP ++SH+QAA+ICS+ H LQIR RSGGHD EGLSY
Sbjct: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120

Query: 121 VAHLPFIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGV 180
           VAHLPFIIIDLINL+SI V++EN+TAW+QSGATLGELYY IA+KSRTLAFPAGVCPTVGV
Sbjct: 121 VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV 180

Query: 181 GGHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIV 240
           GGHFSGGGYGL+LRKYGLAADNVIDAYL+DANGKF+DRESMGEDLFWAIRGGGGGSFGIV
Sbjct: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240

Query: 241 VAWKVKLVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVYH-----GGNISTQ 300
           VAWKVKLV VPA VT C  +R L+ED  I L+HRWQ V  KLD+N+Y      GGN ST+
Sbjct: 241 VAWKVKLVPVPAIVTTCSSDRNLDED-TINLIHRWQSVAYKLDENLYFGIILAGGNASTE 300

Query: 301 GG--KTSPRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAG-GFQIG 360
           GG  K +P A FFSLFLGK DEL+AIL + FPEL L +K C+ETSWIES L+A    Q  
Sbjct: 301 GGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTI 360

Query: 361 EPLEALLNRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMG 420
           E LE+LLNRT  T  + K+KSDYVKEPI +A+IEGIWERLK+QDIE  Q++FVPYGGRM 
Sbjct: 361 ESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMS 420

Query: 421 RISESETPFSHRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYV 480
           RIS+SETPFSHR  NLYKIGY +GWKE  + AE+RHLSWIR +YDYM PFVSKSPR+ YV
Sbjct: 421 RISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYV 480

Query: 481 NYRDLDIGSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPPL 531
           NYRDLDIG+NNKYGKT YKQASVWG KYFGNNFNRLV +KSKVDP N F +EQSIPPL
Sbjct: 481 NYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537

BLAST of Spg035763 vs. ExPASy TrEMBL
Match: A0A6J1KHF6 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111495284 PE=3 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 1.1e-229
Identity = 393/529 (74.29%), Postives = 452/529 (85.44%), Query Frame = 0

Query: 7   ISLALAF-IVLSSSSSWVTASTNKHQYFLQCLSDHSSSSSSISKVVYTPINSSYSSVLNF 66
           I L L F IVLSSS  W + S +KHQ+FL CLS HS  +  ISK+VYTPINSSYS VLNF
Sbjct: 4   IPLTLIFIIVLSSSPLWASPSLHKHQHFLHCLSHHSPHTYPISKLVYTPINSSYSPVLNF 63

Query: 67  SIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAHLP 126
           SIRNL FS  +TPKPL+IITP HISHIQAAI+CS+ HDLQIRTRSGGHDFEGLSYVA+ P
Sbjct: 64  SIRNLIFSNPQTPKPLLIITPSHISHIQAAIVCSKAHDLQIRTRSGGHDFEGLSYVANQP 123

Query: 127 FIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGHFS 186
           FII+DLINLRSI+VDV+ NTAWVQSGATLGELYYRI E SR+LAFPAG+CPTVGVGGHFS
Sbjct: 124 FIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGETSRSLAFPAGICPTVGVGGHFS 183

Query: 187 GGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 246
           GGGYGL+LRKYGLAADNV+DAYLVDANG+ HDRESMGEDLFWAIRGGGGGSFGIVVAWKV
Sbjct: 184 GGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 243

Query: 247 KLVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVY-----HGGNISTQGGKTS 306
           KLV VPA VT+C  NR +E+D AI+L+HRWQYV N+LDDN++      GGN ST+ G T+
Sbjct: 244 KLVMVPAVVTMCSTNRDVEDD-AIKLIHRWQYVANQLDDNLFLGILLTGGNFSTREGITN 303

Query: 307 PRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAG-GFQIGEPLEALL 366
           P A FFSL+LGK+DEL+ ILK+ F ELGLT+K C+E+SWI+S LIA  G Q  EPLEALL
Sbjct: 304 PTATFFSLYLGKLDELITILKNNFSELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALL 363

Query: 367 NRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESET 426
           NRT  T  S K KSDYVK+PI +A+IEGIW+RLK+QDIE  Q++F+PYGGRM +ISESET
Sbjct: 364 NRTPPTIESTKFKSDYVKDPIPEATIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESET 423

Query: 427 PFSHRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLDI 486
           PFSHR GNLYKIGY + WKE  +DA+ +H++WIR VY+YM PFVSKSPR+AY NYRDLDI
Sbjct: 424 PFSHRAGNLYKIGYAVSWKEQSLDAKNKHINWIREVYEYMTPFVSKSPRAAYANYRDLDI 483

Query: 487 GSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIP 529
           G+NNKYGKT Y+QASVWG+KYFGNNFNRLV VK+KVDP++ F HEQSIP
Sbjct: 484 GTNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

BLAST of Spg035763 vs. ExPASy TrEMBL
Match: A0A5A7TU57 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00190 PE=3 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 2.0e-228
Identity = 394/532 (74.06%), Postives = 454/532 (85.34%), Query Frame = 0

Query: 5   SLISLALAFIVLSSSSSWVTASTNKHQYFLQCLSDHSSSSSSISKVVYTPINSSYSSVLN 64
           +LI L+L FIV+SS+S   +AS  K+Q FLQCLS+HSS S  ISKVVYTPINSSYSSVL+
Sbjct: 6   TLIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSYSSVLD 65

Query: 65  FSIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAHL 124
           FSIRNL FS  +TPKPL+IITP H+SHIQAA+ICS++H LQIRTRSGGHDFEGLSYVA+ 
Sbjct: 66  FSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYR 125

Query: 125 PFIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGHF 184
           PFI++DLINLRS+ V+VENNTAWV+SGATLGELYYRI EKSRTLAFPAGVCPTVG+GGHF
Sbjct: 126 PFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHF 185

Query: 185 SGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWK 244
           SGGGYGL+LRK+GLAADNVIDAYLVDANGK  D+ESMGEDLFWAIRGGGGGSFGIVVAWK
Sbjct: 186 SGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVAWK 245

Query: 245 VKLVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVY-----HGGNISTQGGKT 304
           +KLV VP TVT+C  +R LEED AI+L+HRWQYVGNKLD+N+Y      GGN STQ G T
Sbjct: 246 IKLVPVPETVTICSTDRNLEED-AIKLIHRWQYVGNKLDENIYLGIILTGGNASTQAGIT 305

Query: 305 SPRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAG-GFQIGEPLEAL 364
           +P A FFSLFLG+ DELV  L + FPELGL ++ C+E SW+ES LI   G Q  E LE L
Sbjct: 306 NPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEPL 365

Query: 365 LNRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESE 424
           LNRTL T  S KIKSDY+ EPI  A+I+ IW+RLK+QDIE+SQ++FVPYGGRM +ISESE
Sbjct: 366 LNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQISESE 425

Query: 425 TPFSHRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLD 484
           TPFSHR   L+KIGY +GWK+  + A+KRH+SW+R VY+YMAPFVSKSPR+AY NYRDLD
Sbjct: 426 TPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNYRDLD 485

Query: 485 IGSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPPL 531
           IGSNNKYGKT YK+ASVWGLKYFGNNF+RLV VK+KVDPY+ F HEQSIP L
Sbjct: 486 IGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Spg035763 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 533.9 bits (1374), Expect = 1.5e-151
Identity = 272/535 (50.84%), Postives = 373/535 (69.72%), Query Frame = 0

Query: 1   MKTFSLISLALAFI----VLSSSSSWVTASTNKHQYFLQCLSDHSSSSSSISKVVYTPIN 60
           MK  S  S  L F     +L S    V+A+ +    FLQCLS   + S+ +SKV++TP +
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPND 60

Query: 61  SSYSSVLNFSIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFE 120
           +S+SSVL  SI+N RFS    PKP++I+TP+  S +Q+A+ C+R   + IRTRSGGHD+E
Sbjct: 61  TSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYE 120

Query: 121 GLSYVAHLPFIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCP 180
           GLSYV H PF+I+DL NLRSI VDV+N + WVQ+GAT+GELYY I +K+RTLAFPAGVCP
Sbjct: 121 GLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCP 180

Query: 181 TVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGS 240
           TVGVGGHFSGGGYG LLRK+GLAAD+VIDA +VDA G+  +R  MGED FWAIRGGGG S
Sbjct: 181 TVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSS 240

Query: 241 FGIVVAWKVKLVAVPATVTVCYYNRT-LEEDGAIELVHRWQYVGNKLDDNVYHGGNISTQ 300
           F +V++WK+ L+ VP+TVTV  +N T   E  A++++HRWQ+V +K+ D+++    +  Q
Sbjct: 241 FCVVLSWKIGLINVPSTVTV--FNVTKFSEQSALKIIHRWQFVADKVSDDLFI--RVMLQ 300

Query: 301 GGKTSPRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAGGFQIG-EP 360
             K   RA+F  L+LG V  L+ ++   FPELGL E  C E SWIES +     ++G EP
Sbjct: 301 RYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA--ELGEEP 360

Query: 361 LEALLNRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRI 420
           +  L  RT +   + K KSD+V+EP+ + +I  +W RL+  + E +Q+IF P+GG+M  I
Sbjct: 361 INVLTKRT-RASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEI 420

Query: 421 SESETPFSHRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNY 480
           ++ ETPF HR GN+Y+I Y   W+    D +++++ W+  VYD M+ FV+KSPR AY+N 
Sbjct: 421 ADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINL 480

Query: 481 RDLDIGSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPP 530
           RDLD+G      +++Y++   WG+KYF NNF RLV+VK+ VDP + F  EQSIPP
Sbjct: 481 RDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of Spg035763 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 521.9 bits (1343), Expect = 5.9e-148
Identity = 255/513 (49.71%), Postives = 362/513 (70.57%), Query Frame = 0

Query: 20  SSWVTASTNKHQYFLQCLSDHSSSSSSISKVVYTPINSSYSSVLNFSIRNLRFSTAKTPK 79
           S  V+A  + H+ FL+CLS   + ++   KV++T  +SS+ S+L+ SI+N RFS ++TPK
Sbjct: 18  SPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPK 77

Query: 80  PLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAH-LPFIIIDLINLRSID 139
           P+ IITP+  S +Q  I C++ H + +RTRS GH +EGLSY+A+  PF +IDL NLRSI 
Sbjct: 78  PVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSIS 137

Query: 140 VDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSGGGYGLLLRKYGL 199
           +DV+N T WVQ+GAT GELYY I + +++LAFPAG+ PTVGVGG FSGGGYG LLRKYGL
Sbjct: 138 LDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGL 197

Query: 200 AADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVAVPATVTVCY 259
           AADN+IDA +VDA+G+  DR++MGED FWAIRGGGG SFG++++WKVKLV VP+T+TV  
Sbjct: 198 AADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFK 257

Query: 260 YNRTLEEDGAIELVHRWQYVGNKLDDNVYHGGNISTQGGKTSPRAAFFSLFLGKVDELVA 319
             +T +++ A+ ++ +WQY  +K+ D+++    +  +  K +  A F  L++G V+ L+A
Sbjct: 258 VQKTSKKE-AVRIIKKWQYAADKVPDDLFIRTTLE-RSNKNAVHALFTGLYIGPVNNLLA 317

Query: 320 ILKSTFPELGLTEKACIETSWIESALIAGGFQIGEPLEALLNRTLQTHGSIKIKSDYVKE 379
           +++  FPELGL ++ C E SWIES L    F  GE L  L NR  +T  S K K D+V+E
Sbjct: 318 LMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLTNRE-RTSLSFKGKDDFVQE 377

Query: 380 PISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESETPFSHRVGNLYKIGYTIGWK 439
           PI +A+I+ IW RL++ +    ++I  P+GG+M  ++E ETPF HR GNLY+I Y   W+
Sbjct: 378 PIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWR 437

Query: 440 E---PGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLDIGSNNKYGKTRYKQASV 499
           E          ++L W+  VY++M P+VSKSPR AYVN++D+D+G      KT+Y++   
Sbjct: 438 EEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKKTKYEEGKS 497

Query: 500 WGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIP 529
           WG+KYF NNF RLV+VK++VDP + F  EQSIP
Sbjct: 498 WGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Spg035763 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 516.5 bits (1329), Expect = 2.5e-146
Identity = 267/529 (50.47%), Postives = 352/529 (66.54%), Query Frame = 0

Query: 9   LALAFIVLSSSSSWVTASTNKHQYFLQCLSDHSSSSSSISKVVYTPINSSYSSVLNFSIR 68
           L L F+V    SS   +S+   + F QCL+ +S     IS  ++   N SYSSVL  +IR
Sbjct: 7   LVLFFVVFIWQSS---SSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIR 66

Query: 69  NLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVAH--LPF 128
           NLRF+T  TPKP +II   H SH+QAAI C + H+LQ++ RSGGHD++GLSYV +   PF
Sbjct: 67  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 126

Query: 129 IIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVGGHFSG 188
            ++D+ NLRS+DVDV + TAWVQ+GA LGE+YY I EKS+TLA+PAG+CPTVGVGGH SG
Sbjct: 127 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 186

Query: 189 GGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVK 248
           GGYG ++RKYGL  DN IDA +VD NGK  DR+ MGEDL+WAI GGGGGS+G+V+A+K+ 
Sbjct: 187 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 246

Query: 249 LVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVYHGGNISTQGGKTSP----R 308
           LV VP  VTV   +RTLE++ A +++HRWQ V  KL D ++    I    G  S     R
Sbjct: 247 LVEVPENVTVFRISRTLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVR 306

Query: 309 AAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAGGFQIGEPLEALLNRT 368
             F ++FLG    L++IL   FPELGL    C ETSWI+S L     Q+G     LL R 
Sbjct: 307 TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN 366

Query: 369 LQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESETPFS 428
            Q    +K KSDYV+EPIS+  +E IW+  K  ++E   + F PYGG MGRIS + TPF 
Sbjct: 367 -QPVNYLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTVTPFP 426

Query: 429 HRVGNLYKIGYTIGWKEPGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLDIGSN 488
           +R GNL+KI Y   W++  +    R++   R +Y +M PFVSK+PR ++ NYRD+D+G N
Sbjct: 427 YRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGIN 486

Query: 489 NKYGK-TRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPPL 531
           +  GK + Y +   +G KYF  NF RLV++K++VD  N F +EQSIP L
Sbjct: 487 SHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Spg035763 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.6e-145
Identity = 259/531 (48.78%), Postives = 370/531 (69.68%), Query Frame = 0

Query: 6   LISLALAFIVLSSSSSW---VTASTNKHQYFLQCLSDHSSSSSSISKVVYTPINSSYSSV 65
           L+SL + F++L+ S S    ++A    H+ FL+CLS   +     S++++T  + SY S+
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDD--SRIIHTSKDPSYFSI 66

Query: 66  LNFSIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYVA 125
           LN SI+N RF   +TPKP+ IITP+  + +Q+ I C+R H + IRTRSGGHD+EGLSY+A
Sbjct: 67  LNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMA 126

Query: 126 -HLPFIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVGVG 185
              PF++IDL NLRSI +DV+N T WVQSGAT+GELYY I + S++LAFPAG+ PTVG+G
Sbjct: 127 KSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIG 186

Query: 186 GHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVV 245
           G F GGGYG L+RKYGL+ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V+
Sbjct: 187 GQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVL 246

Query: 246 AWKVKLVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVYHGGNISTQGGKTSP 305
           +WK++L+ VP+ VTV    +T E++ A+ ++++WQY+ +K+ ++++    +  +   T  
Sbjct: 247 SWKIRLLDVPSVVTVFKVVKTSEKE-AVSIINKWQYIADKVPNDLFIRAMLQKE---TEV 306

Query: 306 RAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAGGFQIGEPLEALLNR 365
            A+F  L+LG V +L+A++K  FPELGL    C E SWIES L    F  GE +E L  R
Sbjct: 307 YASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL---WFIKGESMEILAKR 366

Query: 366 TLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISESETPF 425
             +T  S K K D+++EPI + +I+ +W R ++ +   +++I  P+GG+M  I+++E PF
Sbjct: 367 K-RTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPF 426

Query: 426 SHRVGNLYKIGYTIGWKE---PGMDAEKRHLSWIRGVYDYMAPFVSKSPRSAYVNYRDLD 485
            HR GNLY+I Y   W E         +++L W+  VY++M P+VSKSPR AYVN+RD+D
Sbjct: 427 PHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDID 486

Query: 486 IGSNNKYG-KTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIP 529
           +G       KT+Y++A VWG+KYF NNF+RLV+VK+ VDP + F  EQSIP
Sbjct: 487 LGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of Spg035763 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 512.7 bits (1319), Expect = 3.6e-145
Identity = 257/538 (47.77%), Postives = 362/538 (67.29%), Query Frame = 0

Query: 4   FSLISLALAFIVLSSSSSWVTASTNKHQYFLQCLSDHSSSSSSISKVVYTPIN--SSYSS 63
           FS++ L  +   ++ +SS  +        F+ C+  ++     + K  + P    S +S 
Sbjct: 10  FSILVLYFSLYTITPTSSLASL----QDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQ 69

Query: 64  VLNFSIRNLRFSTAKTPKPLIIITPLHISHIQAAIICSRNHDLQIRTRSGGHDFEGLSYV 123
           VL  + +NLRF     PKP  I +P+H SH+QA+IICS+   + +R RSGGHD+EGLSYV
Sbjct: 70  VLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYV 129

Query: 124 AHL--PFIIIDLINLRSIDVDVENNTAWVQSGATLGELYYRIAEKSRTLAFPAGVCPTVG 183
           + +  PFI++DL  +R +++++++N+AWVQSGAT+GELYYRIAEKS+   FPAG+C ++G
Sbjct: 130 SQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLG 189

Query: 184 VGGHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGI 243
           +GGH +GG YG ++RKYGL ADNV+DA +VDANGK  DR +MGED FWAIRGG GGSFGI
Sbjct: 190 IGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGI 249

Query: 244 VVAWKVKLVAVPATVTVCYYNRTLEEDGAIELVHRWQYVGNKLDDNVYHG--GNISTQGG 303
           ++AWK+KLV VP TVTV    +TL++D   +++ +WQ V +KL + ++     N++  GG
Sbjct: 250 ILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGG 309

Query: 304 KTSPRAAFFSLFLGKVDELVAILKSTFPELGLTEKACIETSWIESALIAGGFQIGEPLEA 363
             +   ++ +LFLG    L+ ++K +FPELGLT K CIE SW+ES     GF    P   
Sbjct: 310 NKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNV 369

Query: 364 LL-NRTLQTHGSIKIKSDYVKEPISQASIEGIWERLKSQDIESSQVIFVPYGGRMGRISE 423
           LL  ++     S K KSD+VK PI ++ ++GI+++L  +DI    +I+ PYGG M +I E
Sbjct: 370 LLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGMMAKIPE 429

Query: 424 SETPFSHRVGNLYKIGYTIGWKEPGMDAEK---RHLSWIRGVYDYMAPFVSKSPRSAYVN 483
           S+ PF HR G L+K+ Y   W    +D++K   RH++WIR +Y YM P+VS +PR AYVN
Sbjct: 430 SQIPFPHRKGVLFKVQYVTSW----LDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVN 489

Query: 484 YRDLDIGSNNKYGKTRYKQASVWGLKYFGNNFNRLVQVKSKVDPYNIFTHEQSIPPLI 532
           YRDLD+G N K  KT  KQA VWG  YF NNFNRL+ +K+KVDP N F HEQSIPP++
Sbjct: 490 YRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPMM 537