Spg035646 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg035646
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRetrotrans_gag domain-containing protein
Locationscaffold3: 241281 .. 244047 (+)
RNA-Seq ExpressionSpg035646
SyntenySpg035646
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCGGCTTCGTGTTGCCCTGGGCCCGGCCTCCCTTCGGAAAGTGTTTGCATGGGTCAATAACAAGGTGAATGGGGAGAATGTCTGTAGTAAGTGGGAGTAGGACACGTGTCAACGTACCCTACGGTCTCCACCCCTAGGTCGCACCGTGAGATTCTTATGATCTGCCTGCGTGTCGTCCTGGAGCGACCATCCCTTCGGAGGGCCTGATCATAGGTTTCGGAACACCGCGAACTCCAGAAATGGATAGGGTCACTTAGGTTAATTACCAACAGTTGTCTTTCGTTTTGGTAGCCTGTTGGTCCGTACCTCTGGGATCTGAAAGTGGGAGGGTTACTGTAAACCCGAGACTTTCCTTGGATAATTTCCCTTGTCATCTATTGTGGAAGTTGGACTTAAGTTATGTTGTTTCATTATTGAAGGAAAATGGCAAGCTTTTAGTTCAAGTTGTTTTTGTTTATAACTTTGGAGTTTATTTAAAGAAAAGGAAAATGAAAGGAAAGGAAAAGAAGAAAAGAAAAGAAATGGGAAAGGAAAGAAAACCAAAAAAGAAAAAAAAAGAAGAAAAGAAGAAAAAGAAAAGAAAGGAAATGGGAAATGACAGCAAACCTTCCACCACAGACAATGATCCTTTTCAGCTCATTTTCAGCTCATTTCTCATCATTTTCCATCCCAATTTTCAGAGCACTTGAGGGAGAGAGTGAAGGAAGAAGAAGAAGAAGAATTTCGGGGTTTTGAGGTTGAAGAAGATAGAAAAAGCTCATGCAAAGCCAGCCATAGCAGAATCTGAGTTCAACCTGTCAACCTCTGGTTTTTTATTCGGTTTGAGCTACACATAAGGAACTAAGAGGTGAGGTGAGGTTTTCTTGAAATTTAGTTCATTTTCTAACAAATTTCATGAAAGAAGCTTGAGCTAAATCGTATGAGAAGTTAATGGTTTTTGGAATGGAATACAGAGCACAGGATTTTCGAGCAAAGCAGGAGGATCTCTGGTTTTCTCTGGTTTTTCGAGCATTCTGGGGTGTACAAGTTGAATCAGAAGCTATATTTGGTATTGTTGGAAAGCTTATTCAATTTCCTACAACTTTCATGAAGAATTCGTGAGCCAAAAATGACTTTAAGTAGGTTTAATTTCAGAAGATAGATAAAGGGTAGCAGAGAACCACGAATCTGTGTTGTCTGTGTTCGGGGCTTTTCTGGACAAATCAGTTGGGAATCTCGTTTTCATTTCTTCAGGTAAGTTGTAGAGTGTTCGAAAATTAAGAGTTTGATGTATAGTACATTAATTTTGGTTTAAGTATTGAGGAAGTTATGTGTGTTGGACGTTAGGGTATAGAATCTGGAAAATCTGGAAAAGTAGAATGTGAAGTGTGATTTTTAATTCAAGTGTTTAGAATTCATGAGCATGGAAGAATAAGACCATCTATGGTTTTAATGCTCTGTTTTGGTTGTTTGACAGGCCAAGAGGAAATAGGTCGAGTCTACAGACCAGGAAACTAGCTAAGACTGTGAGTGACAAAACGAATTTCGAAAATTGTTTCCAAACGGTTGCACATGTTTAGATGTTTTACATGCTTTTCTATGAATGATACATGGTTTTTATGAATGGTTTCAAGCTTAGGTTATGAGCTTAGATTTTCTAATAAGGTTTATAAATGAGCACGTGATTATTGAGTTTCTGAAAAATAGTTGAAACGACATCCTTTTAAGCCAAGCATGTATTAGCAAGAATTATTTAAAGATTTTCAGTTTTTCTTCCACAAGCAAGCTGAGTAAGCTTGGGTTTTATGAAAGATAACGATAAGCAGGCATGTTCTAATGTTTTCATGTGATTTTCTAAAATTTCTATTGACTATATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCCCACTACGACAAAGACGATGGGAGTGCTGGGCGGGCCCCACTACATCGTAGGAATAGTAAACGTTGGTTGTACTGGGCGTGCCCTACACAATGTAGATCGGTCATGTTAGTTAATATGTTTGATGTACTTGATATGCCTTGCTAGATTTTTAATGACGAACCTGCAATGTTTTTGAGAAAGCTTTTATCATTGCACGTTTATGTTTAACGCCTTGGTTGAGCAGCTTTATACGTTTAAAGTTTTATCACGTGTTCTGATTTCCGAATGTACAGTTTTAAATTTAGTCACTCACTGGGCTTCGTAGCTCATTCTTTCAAAATTTTTTCCCACTTTTCAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTTCAGATCAAGCTCCAGTACGTGGGTGGAGCTGTATATTGAGTCTGTCCATATTGTAAATGTTTTGTAAGGATTGGATAATTTAGTCCTAGAGGTCGTGGTTGTATAAACCCCTATTGTATATGTTTTTGGTTGGGATATCTATATCAGGTTATTTTCCAAACCGAGTTATGTCAGGGCGGCACCTCGTGTATGTTGAAAGGTTTTCAGGGATTCCGTTGTGTTGTGTTTCACGTAGAATATCTTATATCTAAGATTAGTATGTTTATCAGGTTCAACGTTTCAGGAGGGTCAATGGGTATCGTTAGAGGAGGACGATGTCCGTTGGCTTCACGCCATCTTTCGGACTAAGCTAGCAGGTGGTTCGGGAGGGGGTGTGACAATTACACTTACGAGGATGAGTTAA

mRNA sequence

ATGCTCGGCTTCGTGTTGCCCTGGGCCCGGCCTCCCTTCGGAAAGTGTTTGCATGGGTCAATAACAAGAGCACAGGATTTTCGAGCAAAGCAGGAGGATCTCTGGTTTTCTCTGGTTTTTCGAGCATTCTGGGGTGTACAAGTTGAATCAGAAGCTATATTTGGTTATATTCCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTTCAGATCAAGCTCCAGTTCAACGTTTCAGGAGGGTCAATGGGTATCGTTAGAGGAGGACGATGTCCGTTGGCTTCACGCCATCTTTCGGACTAAGCTAGCAGGTGGTTCGGGAGGGGGTGTGACAATTACACTTACGAGGATGAGTTAA

Coding sequence (CDS)

ATGCTCGGCTTCGTGTTGCCCTGGGCCCGGCCTCCCTTCGGAAAGTGTTTGCATGGGTCAATAACAAGAGCACAGGATTTTCGAGCAAAGCAGGAGGATCTCTGGTTTTCTCTGGTTTTTCGAGCATTCTGGGGTGTACAAGTTGAATCAGAAGCTATATTTGGTTATATTCCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTTCAGATCAAGCTCCAGTTCAACGTTTCAGGAGGGTCAATGGGTATCGTTAGAGGAGGACGATGTCCGTTGGCTTCACGCCATCTTTCGGACTAAGCTAGCAGGTGGTTCGGGAGGGGGTGTGACAATTACACTTACGAGGATGAGTTAA

Protein sequence

MLGFVLPWARPPFGKCLHGSITRAQDFRAKQEDLWFSLVFRAFWGVQVESEAIFGYIPLLTLSVLRDNDAVVEIELPVPDTLPTSAESFRSSSSSTFQEGQWVSLEEDDVRWLHAIFRTKLAGGSGGGVTITLTRMS
Homology
[Close]
BLAST of Spg035646 vs. NCBI nr
Match: KAA0060199.1 (uncharacterized protein E6C27_scaffold386G00120 [Cucumis melo var. makuwa] >TYJ95571.1 uncharacterized protein E5676_scaffold767G00770 [Cucumis melo var. makuwa])

HSP 1 Score: 97.4 bits (241), Expect = 9.9e-17
Identity = 53/79 (67.09%), Postives = 58/79 (73.42%), Query Frame = 0 

Query: 59  LLTLSVLRDNDAVVEIELPVPDTLPTSAESFRSSSSSTFQEGQWVSLEEDDVRWLHAIFR 118
           L TLSVLRDNDAVVEIELPVPDTLPTSAESF S+S ST        +  DDV WLHA+FR
Sbjct: 75  LSTLSVLRDNDAVVEIELPVPDTLPTSAESFESNSRST------GIVRGDDVCWLHAVFR 134

Query: 119 TKLAGGSGGGVTITLTRMS 138
            K AGG GGGVT  + + S
Sbjct: 135 AKTAGGPGGGVTFNIAKGS 147

[Close]
BLAST of Spg035646 vs. NCBI nr
Match: TYK27998.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 95.5 bits (236), Expect = 3.8e-16
Identity = 53/82 (64.63%), Postives = 60/82 (73.17%), Query Frame = 0 

Query: 58  PLLTLSVLRDNDAVVEIELPVPDTLPTSAESFRSSSSSTFQ--------EGQWVS--LEE 117
           PL TLSVLRDNDAVVEIELPVPDTLPTSAES  S+SS+  +        E  W++  + E
Sbjct: 20  PLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLELYVEFVHVEMFWLAGIVRE 79

Query: 118 DDVRWLHAIFRTKLAGGSGGGV 130
           DDV WLHA+FR K AGG GGGV
Sbjct: 80  DDVCWLHAVFRAKTAGGPGGGV 101

[Close]
BLAST of Spg035646 vs. NCBI nr
Match: KAA0066851.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 95.5 bits (236), Expect = 3.8e-16
Identity = 53/82 (64.63%), Postives = 60/82 (73.17%), Query Frame = 0 

Query: 58  PLLTLSVLRDNDAVVEIELPVPDTLPTSAESFRSSSSSTFQ--------EGQWVS--LEE 117
           PL TLSVLRDNDAVVEIELPVPDTLPTSAES  S+SS+  +        E  W++  + E
Sbjct: 20  PLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLELYVEFVHVEMFWLAGIVRE 79

Query: 118 DDVRWLHAIFRTKLAGGSGGGV 130
           DDV WLHA+FR K AGG GGGV
Sbjct: 80  DDVCWLHAVFRAKTAGGPGGGV 101

[Close]
BLAST of Spg035646 vs. NCBI nr
Match: TYK00845.1 (zf-CCHC domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 88.6 bits (218), Expect = 4.6e-14
Identity = 55/102 (53.92%), Postives = 65/102 (63.73%), Query Frame = 0 

Query: 37  SLVFRAFWGVQ--------VESEAIFGYIPLLTLSVLRDNDAVVEIELPVPDTLPTSAES 96
           +L+ + F G++        V ++ IF   PL TLSVLRDNDAV EIELPVPDTL TSAES
Sbjct: 25  NLIVKKFMGMEGQKLSMGTVCTQVIF---PLSTLSVLRDNDAVAEIELPVPDTLSTSAES 84

Query: 97  FRSSSSSTFQEGQWVSLEEDDVRWLHAIFRTKLAGGSGGGVT 131
             S+S S         +  DDV WLHAIFR K+ GG GGGVT
Sbjct: 85  SGSNSRSA------GIVRGDDVCWLHAIFRAKIVGGPGGGVT 117

[Close]
BLAST of Spg035646 vs. NCBI nr
Match: TYK25935.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 87.8 bits (216), Expect = 7.8e-14
Identity = 53/99 (53.54%), Postives = 58/99 (58.59%), Query Frame = 0 

Query: 58  PLLTLSVLRDNDAVVEIELPVPDTLPTSAESFRSSSSSTFQ------------------- 117
           PL TLSVLRDNDAVVEIELP+PDTLPTSAES  S+SS+  +                   
Sbjct: 322 PLSTLSVLRDNDAVVEIELPLPDTLPTSAESSGSNSSTWLELYFESVHVEMLFYKPSDAR 381

Query: 118 -------EGQWVSLEEDDVRWLHAIFRTKLAGGSGGGVT 131
                  EG    +  DDV WLHAIFR K AGG GGGVT
Sbjct: 382 AASYVCWEGSAGIVRGDDVCWLHAIFRAKTAGGPGGGVT 420

[Close]
BLAST of Spg035646 vs. ExPASy TrEMBL
Match: A0A5D3BAD1 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold767G00770 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 4.8e-17
Identity = 53/79 (67.09%), Postives = 58/79 (73.42%), Query Frame = 0 

Query: 59  LLTLSVLRDNDAVVEIELPVPDTLPTSAESFRSSSSSTFQEGQWVSLEEDDVRWLHAIFR 118
           L TLSVLRDNDAVVEIELPVPDTLPTSAESF S+S ST        +  DDV WLHA+FR
Sbjct: 75  LSTLSVLRDNDAVVEIELPVPDTLPTSAESFESNSRST------GIVRGDDVCWLHAVFR 134

Query: 119 TKLAGGSGGGVTITLTRMS 138
            K AGG GGGVT  + + S
Sbjct: 135 AKTAGGPGGGVTFNIAKGS 147

[Close]
BLAST of Spg035646 vs. ExPASy TrEMBL
Match: A0A5D3DX39 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G002140 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.8e-16
Identity = 53/82 (64.63%), Postives = 60/82 (73.17%), Query Frame = 0 

Query: 58  PLLTLSVLRDNDAVVEIELPVPDTLPTSAESFRSSSSSTFQ--------EGQWVS--LEE 117
           PL TLSVLRDNDAVVEIELPVPDTLPTSAES  S+SS+  +        E  W++  + E
Sbjct: 20  PLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLELYVEFVHVEMFWLAGIVRE 79

Query: 118 DDVRWLHAIFRTKLAGGSGGGV 130
           DDV WLHA+FR K AGG GGGV
Sbjct: 80  DDVCWLHAVFRAKTAGGPGGGV 101

[Close]
BLAST of Spg035646 vs. ExPASy TrEMBL
Match: A0A5A7VMG9 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold271G002220 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.8e-16
Identity = 53/82 (64.63%), Postives = 60/82 (73.17%), Query Frame = 0 

Query: 58  PLLTLSVLRDNDAVVEIELPVPDTLPTSAESFRSSSSSTFQ--------EGQWVS--LEE 117
           PL TLSVLRDNDAVVEIELPVPDTLPTSAES  S+SS+  +        E  W++  + E
Sbjct: 20  PLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLELYVEFVHVEMFWLAGIVRE 79

Query: 118 DDVRWLHAIFRTKLAGGSGGGV 130
           DDV WLHA+FR K AGG GGGV
Sbjct: 80  DDVCWLHAVFRAKTAGGPGGGV 101

[Close]
BLAST of Spg035646 vs. ExPASy TrEMBL
Match: A0A5D3BNX1 (Zf-CCHC domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold509G00060 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 2.2e-14
Identity = 55/102 (53.92%), Postives = 65/102 (63.73%), Query Frame = 0 

Query: 37  SLVFRAFWGVQ--------VESEAIFGYIPLLTLSVLRDNDAVVEIELPVPDTLPTSAES 96
           +L+ + F G++        V ++ IF   PL TLSVLRDNDAV EIELPVPDTL TSAES
Sbjct: 25  NLIVKKFMGMEGQKLSMGTVCTQVIF---PLSTLSVLRDNDAVAEIELPVPDTLSTSAES 84

Query: 97  FRSSSSSTFQEGQWVSLEEDDVRWLHAIFRTKLAGGSGGGVT 131
             S+S S         +  DDV WLHAIFR K+ GG GGGVT
Sbjct: 85  SGSNSRSA------GIVRGDDVCWLHAIFRAKIVGGPGGGVT 117

[Close]
BLAST of Spg035646 vs. ExPASy TrEMBL
Match: A0A5A7T9P7 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1164G00060 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 3.8e-14
Identity = 53/99 (53.54%), Postives = 58/99 (58.59%), Query Frame = 0 

Query: 58  PLLTLSVLRDNDAVVEIELPVPDTLPTSAESFRSSSSSTFQ------------------- 117
           PL TLSVLRDNDAVVEIELP+PDTLPTSAES  S+SS+  +                   
Sbjct: 322 PLSTLSVLRDNDAVVEIELPLPDTLPTSAESSGSNSSTWLELYFESVHVEMLFYKPSDAR 381

Query: 118 -------EGQWVSLEEDDVRWLHAIFRTKLAGGSGGGVT 131
                  EG    +  DDV WLHAIFR K AGG GGGVT
Sbjct: 382 AASYVCWEGSAGIVRGDDVCWLHAIFRAKTAGGPGGGVT 420

The following BLAST results are available for this feature:
BLAST of Spg035646 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0060199.19.9e-1767.09uncharacterized protein E6C27_scaffold386G00120 [Cucumis melo var. makuwa] >TYJ9... [more]
TYK27998.13.8e-1664.63reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0066851.13.8e-1664.63reverse transcriptase [Cucumis melo var. makuwa][more]
TYK00845.14.6e-1453.92zf-CCHC domain-containing protein [Cucumis melo var. makuwa][more]
TYK25935.17.8e-1453.54gag/pol protein [Cucumis melo var. makuwa][more]
BLAST of Spg035646 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Spg035646 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3BAD14.8e-1767.09Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5D3DX391.8e-1664.63Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38... [more]
A0A5A7VMG91.8e-1664.63Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27... [more]
A0A5D3BNX12.2e-1453.92Zf-CCHC domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A5A7T9P73.8e-1453.54Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1164G000... [more]
BLAST of Spg035646 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg035646.1Spg035646.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
molecular_function GO:0005488 binding
molecular_function GO:0016779 nucleotidyltransferase activity