Spg035626 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg035626
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cytochrome b5
Location	scaffold3: 1833861 .. 1836573 (-)
RNA-Seq Expression	Spg035626
Synteny	Spg035626

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAATGCAAAACCCATCGTTTTGCAGAAGAGTTGAATTGCCCTTACAGCCACTTGTTTTGAAGTTTCGGGAGATGTTTCAATTGGGAAATTCCCCATTAAATTACCAGTTAAATGCTTGAGTAATTCCTTTGTGTGTCATTCATGGATTCAAACAATTTCAATGATAAATATGTATTGACTACTCTGCCCTCTATTACAGTAGCTTTTCACCTTTTGACTTCGTAAATAACTTGCAACTCAAGGGGCTTTTTGGCGGCCTCGCTCTTCTTCTTTCATCGTTATTATAATACGATTTGTTTCTTGTCAAATCAGGAACTGATCTGTAATCGGGTTTTGCCGCAAACAAGGATCCGAACAGTACGTTGCCTCTTCAACCCTTTTCCATCTGTAAGTGAGCTTGTTTCTTACCGACCCATTTCGATTTCAAAGGTTTTGGTTATTTCTGTTGGGTTTTTCTCCCCTTTTGAATGTATCTAGACTTGGTTGCTGTTCGGTAATTCTACTTTGGCTTTGTTTTTGGATGTGGAACTTCAAGTTCTTGGCCTCTGCTCTTTGTGGGGTTTGTGAATTTCGTTTGTTTCTTGATTGTTGCAGTGATAAATGGCTTCGGATCCGAAGGTTCATCTATTCGAAGAAGTGGCGAAGCACAACAAAACCAAAGATTGTTGGCTTGTAATCTCTGGGAAGGTTGGATCTCTATTAGTTGGTTGTTCGTATCATTCTATTTAGTACAGAACATCAGTTTTCGGGTTTAATTGCTGGGTATTTCTTGTTTGGTTGGCATGAAAAGCATATGGCTATTGTTTTTGGAGAAGGAAAACAAAACAACTCTACCTAAAGAAAAGCTTATGGCTAATGCTTTTGGAAAAGGAAATTGAAAAACTCTCGCTAACTCTGGATTCTCATCGAATATCCGCTAAAAGAGTTTGATATTAGATGCCTTGGCTTTGGGGGTTTCACTGTTTTACATGCATGTTCTCATACATTTGGTTTCAGATTCTCAAATTCAAAACCATCTTCCTTTTTCTTTATTGTTTTGAATCATACCCGAGATTCGATGTATACCTTTAGTAAAGAAGATAATTTAATCTCACTTCTTTTTGTTCTGTTTCTAGTATCCGGATTTGTTTGTATGACTGAAACACGTTTTACTTATCTCTAGTGTCTTAATTTCACTGTTTTGTAGTCATCTCAAATGGGTTGATAACAGTGGAGAGCTGTTGTGATGTTCATATATGGAGTCTGTATAAAGGACTGGCATTGTATTTATATACAATCACATTGACCAAAGAGTCTGTCCAACAAATTTTGAGCTTTGGAAACAACATTTGTTGGTTGAAAATTGTTAGAATGTGTCCTAAACCTCCCTCTTTACCATGAAGTTTCAGAAAATTATTTCCTTTCTTTATAAGAAATCATACTTTCATTGAATTGCTTGTTATAAAATGCTTAGAAGTAATACCCCAACTCCAAGGGTTGCCATGGTTGTGAAGACATGGGTCAACCAGAGTGGTAAAGATATTGAATATGTTATACTCATGGTGAATATTGAGAGCTTTGGGGATCTAGCTAGAAGTTTATAATGCTGATAAATAGAGATGAGGTAAATTAGAAATTCTTGACCTGCCCTCGCTAAGATGTTTGGAGCCCCAGCCACCAGGGGTTGACTCAATAGTAAAAAAGCAACTTAAATTTAGATGCCTTTTAAATCAAGTGTTCAAATCATGGTAGTTACATTCACAGAATATTAACTATTCTGTAAGTTTTCCAACACCAAGTGCCTTAGAAATCATGTGTTCAAAATGAGATGCTACTAAGCTGGCTTGTCGTGTATCATAAGTAATTTATGTTCTGTGGATTTAAGATGTCTTTTTCTCATGTTTGATTGTTTGTGGTCTTGGCTTTAGGTTTATGATGTAACTCCTTTCATGGACGATCATCCTGGAGGTGATGAAGTACTATTGTCTGCAACTGGTAAGCCTTAGAGTTCCCTTGTTGAAACACTTGATTCTGTTTCTTTCAGGTCTCTTGCAAATGATGATTAGGATGGATATGACAACAGTTATTCATTGGAATGTTTTGCAGGGAAAGATGCCACGAACGATTTCGAAGATGTTGGGCACAGCGATTCGGCAAGAGAAATGATGGACAAGTATTTCATCGGAGAAATTGATCCTTCGACGGTTCCTTTGAAGAAGATTTTCATTCCATCACAACAAACCCAGTACAACCCAGATAAGACACCTGAGTTTGTGATTAAGATCTTGCAGTTTCTGGTTCCCATATTGATTTTGGGTTTGGCATTTGCTGTTCGACACTACACCAAGAATGAATGAATAAACCCTGTGGAGGGTAAAGTGCTTACTCACATCGATTATGTTTGGTAGAAAGGACGTTCCCTTTTTCTATTTTAGCTTCTTTTGGCTTGTGTTTTCATATGGAAGGTTAGTCGTAGACTAGAGTTGTCTGCATCGGATGTTGTATTCTACTTGATAATAAGCTAAGGGCTATCTTTTTTTTTTCCCCATCTTGATTTGCTTTGCTTTTCTTTTTCCATTCTGCATCTTTGAAAGTGTTAGCAAATTTGGGTTACATCCAAAAATTGTGAACTCAGGAAATGACTATTTGTAATCTTTAACGATGCCATGACTTGTATGTTTCTAAATCGACTTGTGACAAATATTGGTATTTAAACAAATAAGAGAATAT

mRNA sequence

ATGGTTGTGAAGACATGGGTCAACCAGAGTGGTAAAGATATTGAATATGTTATACTCATGGTTTATGATGTAACTCCTTTCATGGACGATCATCCTGGAGGTGATGAAGTACTATTGTCTGCAACTGGGAAAGATGCCACGAACGATTTCGAAGATGTTGGGCACAGCGATTCGGCAAGAGAAATGATGGACAAGTATTTCATCGGAGAAATTGATCCTTCGACGGTTCCTTTGAAGAAGATTTTCATTCCATCACAACAAACCCAGTACAACCCAGATAAGACACCTGAGTTTGTGATTAAGATCTTGCAGTTTCTGGTTCCCATATTGATTTTGGGTTTGGCATTTGCTGTTCGACACTACACCAAGAATGAATGA

Coding sequence (CDS)

ATGGTTGTGAAGACATGGGTCAACCAGAGTGGTAAAGATATTGAATATGTTATACTCATGGTTTATGATGTAACTCCTTTCATGGACGATCATCCTGGAGGTGATGAAGTACTATTGTCTGCAACTGGGAAAGATGCCACGAACGATTTCGAAGATGTTGGGCACAGCGATTCGGCAAGAGAAATGATGGACAAGTATTTCATCGGAGAAATTGATCCTTCGACGGTTCCTTTGAAGAAGATTTTCATTCCATCACAACAAACCCAGTACAACCCAGATAAGACACCTGAGTTTGTGATTAAGATCTTGCAGTTTCTGGTTCCCATATTGATTTTGGGTTTGGCATTTGCTGTTCGACACTACACCAAGAATGAATGA

Protein sequence

MVVKTWVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE

Homology

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BLAST of Spg035626 vs. NCBI nr
Match: XP_011654591.1 (cytochrome b5 [Cucumis sativus] >KGN49837.1 hypothetical protein Csa_000069 [Cucumis sativus])

HSP 1 Score: 211.8 bits (538), Expect = 3.3e-51
Identity = 105/120 (87.50%), Postives = 110/120 (91.67%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  SGK        VYDVTPFM+DHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK
Sbjct: 23  WLVISGK--------VYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 125
           Y+IGEIDPSTVPLKKIFIPSQQ+Q+NPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE
Sbjct: 83  YYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 134

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BLAST of Spg035626 vs. NCBI nr
Match: XP_008437642.1 (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 211.1 bits (536), Expect = 5.6e-51
Identity = 104/120 (86.67%), Postives = 110/120 (91.67%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  SGK        VYDVTPFM+DHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK
Sbjct: 23  WLVISGK--------VYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 125
           Y+IGEIDPSTVPLKKI+IPSQQTQ+NPDKTPEFVIKILQFLVP+LILGLAFAVRHYTKNE
Sbjct: 83  YYIGEIDPSTVPLKKIYIPSQQTQHNPDKTPEFVIKILQFLVPMLILGLAFAVRHYTKNE 134

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BLAST of Spg035626 vs. NCBI nr
Match: KAA0042530.1 (glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa] >TYK05933.1 glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa])

HSP 1 Score: 209.9 bits (533), Expect = 1.2e-50
Identity = 100/105 (95.24%), Postives = 105/105 (100.00%), Query Frame = 0


Query: 21  VYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYFIGEIDPSTVPLKK 80
           VYDVTPFM+DHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKY+IGEIDPSTVPLKK
Sbjct: 565 VYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKK 624

Query: 81  IFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 126
           I+IPSQQTQ+NPDKTPEFVIKILQFLVP+LILGLAFAVRHYTKNE
Sbjct: 625 IYIPSQQTQHNPDKTPEFVIKILQFLVPMLILGLAFAVRHYTKNE 669

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BLAST of Spg035626 vs. NCBI nr
Match: XP_038875650.1 (cytochrome b5 [Benincasa hispida])

HSP 1 Score: 208.8 bits (530), Expect = 2.8e-50
Identity = 103/120 (85.83%), Postives = 109/120 (90.83%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  SGK        VYDVTPFM+DHPGGDEVLLSATGKDATNDFEDVGHSD+AREMMDK
Sbjct: 23  WLVISGK--------VYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDAAREMMDK 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 125
           Y+IGEIDPSTVP KKI+IPSQQTQ+NPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE
Sbjct: 83  YYIGEIDPSTVPSKKIYIPSQQTQHNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 134

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BLAST of Spg035626 vs. NCBI nr
Match: XP_022922141.1 (cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_022922142.1 cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_023549751.1 cytochrome b5-like [Cucurbita pepo subsp. pepo] >KAG6579306.1 Cytochrome b5 isoform E, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016804.1 Cytochrome b5 isoform E [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 204.9 bits (520), Expect = 4.0e-49
Identity = 101/120 (84.17%), Postives = 107/120 (89.17%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  SGK        VYDVTPFM+DHPGGDEVLL+ATGKDATNDFEDVGHSDSAREMMDK
Sbjct: 23  WLVISGK--------VYDVTPFMEDHPGGDEVLLAATGKDATNDFEDVGHSDSAREMMDK 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 125
           Y+IGEIDPSTVPL+KI+IPSQQTQYNPDKT EFVIKILQ LVPI ILGLAFAVRHYTKNE
Sbjct: 83  YYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFILGLAFAVRHYTKNE 134

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BLAST of Spg035626 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 180.6 bits (457), Expect = 1.1e-44
Identity = 88/120 (73.33%), Postives = 98/120 (81.67%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  SGK        VYDVTPFMDDHPGGDEVLLS+TGKDATNDFEDVGHSD+AR+MMDK
Sbjct: 23  WLIISGK--------VYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHSDTARDMMDK 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 125
           YFIGEID S+VP  + ++  QQ  YN DKTPEF+IKILQFLVPILILGLA  VRHYTK +
Sbjct: 83  YFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILILGLALVVRHYTKKD 134

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BLAST of Spg035626 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.0e-42
Identity = 86/120 (71.67%), Postives = 96/120 (80.00%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  SGK        VYDVTPFMDDHPGGDEVLLS+TGKDATNDFEDVGHSD+AR+MM+K
Sbjct: 23  WLIISGK--------VYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHSDTARDMMEK 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 125
           Y+IGEID STVP  + ++   Q  YN DKTPEF+IKILQFLVPILILGLA  VR YTK E
Sbjct: 83  YYIGEIDSSTVPATRTYVAPVQPAYNQDKTPEFMIKILQFLVPILILGLALVVRQYTKKE 134

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BLAST of Spg035626 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 3.3e-38
Identity = 75/118 (63.56%), Postives = 92/118 (77.97%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  SGK        VYDVT F+DDHPGGDEVLLSATGKDAT+DFEDVGHS SAR M+D+
Sbjct: 23  WLVISGK--------VYDVTKFLDDHPGGDEVLLSATGKDATDDFEDVGHSSSARAMLDE 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTK 124
           Y++G+ID +T+P K  + P  Q  YN DKT EFV+K+LQFLVP++ILG+AF +R YTK
Sbjct: 83  YYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLIILGVAFGIRFYTK 132

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BLAST of Spg035626 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 156.8 bits (395), Expect = 1.6e-37
Identity = 70/105 (66.67%), Postives = 91/105 (86.67%), Query Frame = 0


Query: 21  VYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYFIGEIDPSTVPLKK 80
           VY+V+ F++DHPGGD+VLLS+TGKDAT+DFEDVGHS +AR MMD+Y++G+ID ST+P + 
Sbjct: 31  VYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSTTARAMMDEYYVGDIDTSTIPART 90

Query: 81  IFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 126
            ++P +Q  YN DKTPEF+IKILQFLVP+ ILGLA A+R YTK+E
Sbjct: 91  KYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAVAIRIYTKSE 135

[Close]

BLAST of Spg035626 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 2.1e-37
Identity = 73/119 (61.34%), Postives = 95/119 (79.83%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  +GK        VY+VT F++DHPGGD+VLLS+TGKDAT+DFEDVGHS+SAREMM++
Sbjct: 23  WIVINGK--------VYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSESAREMMEQ 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKN 125
           Y++GEIDP+T+P K  + P +Q  YN DKT EF+IK+LQFLVP+ ILGLA  +R YTK+
Sbjct: 83  YYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLAVGIRIYTKS 133

[Close]

BLAST of Spg035626 vs. ExPASy TrEMBL
Match: A0A0A0KN46 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139160 PE=3 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.6e-51
Identity = 105/120 (87.50%), Postives = 110/120 (91.67%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  SGK        VYDVTPFM+DHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK
Sbjct: 23  WLVISGK--------VYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 125
           Y+IGEIDPSTVPLKKIFIPSQQ+Q+NPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE
Sbjct: 83  YYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 134

[Close]

BLAST of Spg035626 vs. ExPASy TrEMBL
Match: A0A1S3AU68 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103482987 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.7e-51
Identity = 104/120 (86.67%), Postives = 110/120 (91.67%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  SGK        VYDVTPFM+DHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK
Sbjct: 23  WLVISGK--------VYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 125
           Y+IGEIDPSTVPLKKI+IPSQQTQ+NPDKTPEFVIKILQFLVP+LILGLAFAVRHYTKNE
Sbjct: 83  YYIGEIDPSTVPLKKIYIPSQQTQHNPDKTPEFVIKILQFLVPMLILGLAFAVRHYTKNE 134

[Close]

BLAST of Spg035626 vs. ExPASy TrEMBL
Match: A0A5A7TGS6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00250 PE=3 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 6.0e-51
Identity = 100/105 (95.24%), Postives = 105/105 (100.00%), Query Frame = 0


Query: 21  VYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYFIGEIDPSTVPLKK 80
           VYDVTPFM+DHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKY+IGEIDPSTVPLKK
Sbjct: 565 VYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKK 624

Query: 81  IFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 126
           I+IPSQQTQ+NPDKTPEFVIKILQFLVP+LILGLAFAVRHYTKNE
Sbjct: 625 IYIPSQQTQHNPDKTPEFVIKILQFLVPMLILGLAFAVRHYTKNE 669

[Close]

BLAST of Spg035626 vs. ExPASy TrEMBL
Match: A0A6J1E3B3 (cytochrome b5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430208 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.9e-49
Identity = 101/120 (84.17%), Postives = 107/120 (89.17%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  SGK        VYDVTPFM+DHPGGDEVLL+ATGKDATNDFEDVGHSDSAREMMDK
Sbjct: 23  WLVISGK--------VYDVTPFMEDHPGGDEVLLAATGKDATNDFEDVGHSDSAREMMDK 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 125
           Y+IGEIDPSTVPL+KI+IPSQQTQYNPDKT EFVIKILQ LVPI ILGLAFAVRHYTKNE
Sbjct: 83  YYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFILGLAFAVRHYTKNE 134

[Close]

BLAST of Spg035626 vs. ExPASy TrEMBL
Match: A0A6J1CBE4 (cytochrome b5 OS=Momordica charantia OX=3673 GN=LOC111009154 PE=3 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 4.3e-49
Identity = 100/120 (83.33%), Postives = 107/120 (89.17%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  SGK        VYDVTPFM+DHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK
Sbjct: 23  WLVISGK--------VYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 125
           Y+IGEIDPSTVPLK+++IP+QQTQYN DKTPEFVIKILQ LVPI ILGLAFAVRHYTKNE
Sbjct: 83  YYIGEIDPSTVPLKRMYIPAQQTQYNSDKTPEFVIKILQILVPIFILGLAFAVRHYTKNE 134

[Close]

BLAST of Spg035626 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 180.6 bits (457), Expect = 7.6e-46
Identity = 88/120 (73.33%), Postives = 98/120 (81.67%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  SGK        VYDVTPFMDDHPGGDEVLLS+TGKDATNDFEDVGHSD+AR+MMDK
Sbjct: 23  WLIISGK--------VYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHSDTARDMMDK 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKNE 125
           YFIGEID S+VP  + ++  QQ  YN DKTPEF+IKILQFLVPILILGLA  VRHYTK +
Sbjct: 83  YFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILILGLALVVRHYTKKD 134

[Close]

BLAST of Spg035626 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 156.4 bits (394), Expect = 1.5e-38
Identity = 73/119 (61.34%), Postives = 95/119 (79.83%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           W+  +GK        VY+VT F++DHPGGD+VLLS+TGKDAT+DFEDVGHS+SAREMM++
Sbjct: 23  WIVINGK--------VYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSESAREMMEQ 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKN 125
           Y++GEIDP+T+P K  + P +Q  YN DKT EF+IK+LQFLVP+ ILGLA  +R YTK+
Sbjct: 83  YYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLAVGIRIYTKS 133

[Close]

BLAST of Spg035626 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 148.7 bits (374), Expect = 3.2e-36
Identity = 72/121 (59.50%), Postives = 96/121 (79.34%), Query Frame = 0


Query: 8   NQSGKDIEYVI-LMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKY 67
           + S KD   VI   VYDVT F+DDHPGGDEV+L++TGKDAT+DFEDVGHS +A+ M+D+Y
Sbjct: 16  HSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSSTAKAMLDEY 75

Query: 68  FIGEIDPSTVPLKKIFIPSQQTQ--YNPDKTPEFVIKILQFLVPILILGLAFAVRHYTKN 126
           ++G+ID +TVP+K  F+P   T+     DK+ +FVIK+LQFLVP+LILGLAF +R+YTK 
Sbjct: 76  YVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPLLILGLAFGIRYYTKT 135

[Close]

BLAST of Spg035626 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 112.1 bits (279), Expect = 3.3e-25
Identity = 55/103 (53.40%), Postives = 76/103 (73.79%), Query Frame = 0


Query: 21  VYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYFIGEIDPSTVPLKK 80
           VYD++ FMD+HPGGD VLL+ TGKDA+ DFEDV HS  A+E+M KY IG++D STVP+ +
Sbjct: 27  VYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAKELMKKYCIGDVDQSTVPVTQ 86

Query: 81  IFIPSQQTQYNPDKT--PEFVIKILQFLVPILILGLAFAVRHY 122
            +IP  + +    +T   E   K+L +L+P+LILG+AFA+R Y
Sbjct: 87  QYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVAFALRFY 129

[Close]

BLAST of Spg035626 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 95.9 bits (237), Expect = 2.4e-20
Identity = 54/118 (45.76%), Postives = 75/118 (63.56%), Query Frame = 0


Query: 6   WVNQSGKDIEYVILMVYDVTPFMDDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDK 65
           WV   GK        VYDV+ +MD+HPGGD+VLL+  GKDAT+DFED GHS  ARE+M+K
Sbjct: 23  WVVIDGK--------VYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKDARELMEK 82

Query: 66  YFIGEIDPSTVPLKKIFIPSQQTQYNPDKTPEFVIKILQ-----FLVPILILGLAFAV 119
           YFIGE+D S++P     IP  +  Y  D+  + V K+       ++VP+ I+ ++ AV
Sbjct: 83  YFIGELDESSLP----EIPELKI-YKKDQPQDSVQKLFDLTKQYWVVPVSIITISVAV 127

The following BLAST results are available for this feature:

BLAST of Spg035626 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654591.1	3.3e-51	87.50	cytochrome b5 [Cucumis sativus] >KGN49837.1 hypothetical protein Csa_000069 [Cuc...	[more]
XP_008437642.1	5.6e-51	86.67	PREDICTED: cytochrome b5 [Cucumis melo]	[more]
KAA0042530.1	1.2e-50	95.24	glucan endo-1,3-beta-glucosidase [Cucumis melo var. makuwa] >TYK05933.1 glucan e...	[more]
XP_038875650.1	2.8e-50	85.83	cytochrome b5 [Benincasa hispida]	[more]
XP_022922141.1	4.0e-49	84.17	cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_022922142.1 cytochrome b5...	[more]

BLAST of Spg035626 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42342	1.1e-44	73.33	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P40934	1.0e-42	71.67	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
P49098	3.3e-38	63.56	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49100	1.6e-37	66.67	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]
O48845	2.1e-37	61.34	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]

BLAST of Spg035626 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KN46	1.6e-51	87.50	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A1S3AU68	2.7e-51	86.67	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103482987 PE=3 SV=1	[more]
A0A5A7TGS6	6.0e-51	95.24	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A6J1E3B3	1.9e-49	84.17	cytochrome b5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430208 PE=3...	[more]
A0A6J1CBE4	4.3e-49	83.33	cytochrome b5 OS=Momordica charantia OX=3673 GN=LOC111009154 PE=3 SV=1	[more]

BLAST of Spg035626 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53560.1	7.6e-46	73.33	cytochrome B5 isoform E	[more]
AT2G32720.1	1.5e-38	61.34	cytochrome B5 isoform B	[more]
AT5G48810.1	3.2e-36	59.50	cytochrome B5 isoform D	[more]
AT2G46650.1	3.3e-25	53.40	cytochrome B5 isoform C	[more]
AT1G26340.1	2.4e-20	45.76	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 46..53 score: 45.27 coord: 31..45 score: 54.65 coord: 21..31 score: 58.82 coord: 59..71 score: 43.94
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 9..72 e-value: 2.5E-15 score: 66.9
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 21..71 e-value: 6.6E-17 score: 61.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 1..72 score: 23.002768
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 4..75 e-value: 4.1E-23 score: 83.2
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 20..78
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 12..123
None	No IPR available	PANTHER	PTHR19359:SF103	CYTOCHROME B5-LIKE	coord: 12..123
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 27..34

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg035626.1	Spg035626.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0035672	oligopeptide transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0035673	oligopeptide transmembrane transporter activity