Spg035556 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg035556
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein kinase family protein
Locationscaffold3: 3700277 .. 3701341 (+)
RNA-Seq ExpressionSpg035556
SyntenySpg035556
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCATATACATCCGAAGAAGACGTAGACTATCAAGCAAAACATTATCAACATTCAAGAAGAAAATGCTGACTATGGAATTAGTAATGAATTCCAAACCGAAGCGATATCATTATTCAGAGATCAAGAAAATGACCAACTCATTTTCAGACAAACTAGGAGAAGGGGGATACGGTGTTGTGTATCGAGGTAAACTAGATAATGGTACCCTCATAGCTATAAAAGTTCTAAAGGTGTCGAAAAGTGATGGTGAAGATTTCATAAATGAAGTGATGAGTATTAGCAAGACTTCACATGTTAATATAGTTAGCCTTTTAGGAGTTTCTTACAACTCAAAGGAAGTAGCGCTTGTGTATGAATTTATGTCTAATGGCTCACTCGAGAGCTTCATCTCTAGAACTCAAAACCTCGAGATGAAAATCGTTCATAGAATCGTTGTAGGTGTGGCAAAAGGATTAGAATATCTACATTGTGGTTGTAGTACAAGGATAGTCCATTTCGACATAAAGCCTCAAAACATTCTATTAGATGAAGATTTCAATACTAAAATTTCTGACTTTGGCCTTGCAAAGCTTTGCAAAAGAAAGGATAGTGCGATTTCAATGTTAGGTTCAAGAGGAACAGTTGGATTCATGGCACCAGAAGTTTATAGTCGTGCATTTGGAATTGTCTCATACAAATCTGATGTGTATAGCTATGGGATGCTAGTTCTTGATCTTGTATTGGGAGGAAGAAGAAACGTTAGTTCAAAATTTTTATCTGATGATAGTGAAATGTACTTTCCAAATTGGGTTCTTAAAAATTTTGAAGCAGGTAAAACTTTAAGAAGTAATCAGAATTTGACAGAGGAAGAAGAAGAAATGGAGAAGAAAATGGTTATAGTAGGCTTGTGGTGCATTCAAACATCTCCACTTGATAGGCCTTCAATGACTAAAGTATTGGAGATGTTGGGTGGAACCAGTCACTCTTTGCAAATGCCTCCAAGATCTTCATTGGTTCTCCCTGCTGAAATGTCAACTCAACAATCTGTCTCTCAATCATTATCACCAGAAGAGGTTGTTTAA

mRNA sequence

ATGATCATATACATCCGAAGAAGACGTAGACTATCAAGCAAAACATTATCAACATTCAAGAAGAAAATGCTGACTATGGAATTAGTAATGAATTCCAAACCGAAGCGATATCATTATTCAGAGATCAAGAAAATGACCAACTCATTTTCAGACAAACTAGGAGAAGGGGGATACGGTGTTGTGTATCGAGGTAAACTAGATAATGGTACCCTCATAGCTATAAAAGTTCTAAAGGTGTCGAAAAGTGATGGTGAAGATTTCATAAATGAAGTGATGAGTATTAGCAAGACTTCACATGTTAATATAGTTAGCCTTTTAGGAGTTTCTTACAACTCAAAGGAAGTAGCGCTTGTGTATGAATTTATGTCTAATGGCTCACTCGAGAGCTTCATCTCTAGAACTCAAAACCTCGAGATGAAAATCGTTCATAGAATCGTTGTAGGTGTGGCAAAAGGATTAGAATATCTACATTGTGGTTGTAGTACAAGGATAGTCCATTTCGACATAAAGCCTCAAAACATTCTATTAGATGAAGATTTCAATACTAAAATTTCTGACTTTGGCCTTGCAAAGCTTTGCAAAAGAAAGGATAGTGCGATTTCAATGTTAGGTTCAAGAGGAACAGTTGGATTCATGGCACCAGAAGTTTATAGTCGTGCATTTGGAATTGTCTCATACAAATCTGATGTGTATAGCTATGGGATGCTAGTTCTTGATCTTGTATTGGGAGGAAGAAGAAACGTTAGTTCAAAATTTTTATCTGATGATAGTGAAATGTACTTTCCAAATTGGGTTCTTAAAAATTTTGAAGCAGGTAAAACTTTAAGAAGTAATCAGAATTTGACAGAGGAAGAAGAAGAAATGGAGAAGAAAATGGTTATAGTAGGCTTGTGGTGCATTCAAACATCTCCACTTGATAGGCCTTCAATGACTAAAGTATTGGAGATGTTGGGTGGAACCAGTCACTCTTTGCAAATGCCTCCAAGATCTTCATTGGTTCTCCCTGCTGAAATGTCAACTCAACAATCTGTCTCTCAATCATTATCACCAGAAGAGGTTGTTTAA

Coding sequence (CDS)

ATGATCATATACATCCGAAGAAGACGTAGACTATCAAGCAAAACATTATCAACATTCAAGAAGAAAATGCTGACTATGGAATTAGTAATGAATTCCAAACCGAAGCGATATCATTATTCAGAGATCAAGAAAATGACCAACTCATTTTCAGACAAACTAGGAGAAGGGGGATACGGTGTTGTGTATCGAGGTAAACTAGATAATGGTACCCTCATAGCTATAAAAGTTCTAAAGGTGTCGAAAAGTGATGGTGAAGATTTCATAAATGAAGTGATGAGTATTAGCAAGACTTCACATGTTAATATAGTTAGCCTTTTAGGAGTTTCTTACAACTCAAAGGAAGTAGCGCTTGTGTATGAATTTATGTCTAATGGCTCACTCGAGAGCTTCATCTCTAGAACTCAAAACCTCGAGATGAAAATCGTTCATAGAATCGTTGTAGGTGTGGCAAAAGGATTAGAATATCTACATTGTGGTTGTAGTACAAGGATAGTCCATTTCGACATAAAGCCTCAAAACATTCTATTAGATGAAGATTTCAATACTAAAATTTCTGACTTTGGCCTTGCAAAGCTTTGCAAAAGAAAGGATAGTGCGATTTCAATGTTAGGTTCAAGAGGAACAGTTGGATTCATGGCACCAGAAGTTTATAGTCGTGCATTTGGAATTGTCTCATACAAATCTGATGTGTATAGCTATGGGATGCTAGTTCTTGATCTTGTATTGGGAGGAAGAAGAAACGTTAGTTCAAAATTTTTATCTGATGATAGTGAAATGTACTTTCCAAATTGGGTTCTTAAAAATTTTGAAGCAGGTAAAACTTTAAGAAGTAATCAGAATTTGACAGAGGAAGAAGAAGAAATGGAGAAGAAAATGGTTATAGTAGGCTTGTGGTGCATTCAAACATCTCCACTTGATAGGCCTTCAATGACTAAAGTATTGGAGATGTTGGGTGGAACCAGTCACTCTTTGCAAATGCCTCCAAGATCTTCATTGGTTCTCCCTGCTGAAATGTCAACTCAACAATCTGTCTCTCAATCATTATCACCAGAAGAGGTTGTTTAA

Protein sequence

MIIYIRRRRRLSSKTLSTFKKKMLTMELVMNSKPKRYHYSEIKKMTNSFSDKLGEGGYGVVYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMSISKTSHVNIVSLLGVSYNSKEVALVYEFMSNGSLESFISRTQNLEMKIVHRIVVGVAKGLEYLHCGCSTRIVHFDIKPQNILLDEDFNTKISDFGLAKLCKRKDSAISMLGSRGTVGFMAPEVYSRAFGIVSYKSDVYSYGMLVLDLVLGGRRNVSSKFLSDDSEMYFPNWVLKNFEAGKTLRSNQNLTEEEEEMEKKMVIVGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPPRSSLVLPAEMSTQQSVSQSLSPEEVV
Homology
BLAST of Spg035556 vs. NCBI nr
Match: QKK82668.1 (LRK10L2 [Cucumis sativus])

HSP 1 Score: 504.6 bits (1298), Expect = 6.9e-139
Identity = 261/353 (73.94%), Postives = 304/353 (86.12%), Query Frame = 0

Query: 1   MIIYIRRRRRLSSKTLSTFKKKMLTMELVMNSKPKRYHYSEIKKMTNSFSDKLGEGGYGV 60
           MIIYIRRR+RLS+K+ S   +K   ++ ++N  PKRYHYSEIKKMTNSFS K+GEGGYG+
Sbjct: 281 MIIYIRRRKRLSTKSSSISNRKKYGIDSIINYGPKRYHYSEIKKMTNSFSTKIGEGGYGI 340

Query: 61  VYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMSISKTSHVNIVSLLGVSYNSKEVALVYE 120
           VY+GKL +GTL+AIKVLK+SK++GEDFINEVMSIS+TSHVNIV LLG  Y S++ AL+YE
Sbjct: 341 VYQGKLLDGTLVAIKVLKLSKANGEDFINEVMSISRTSHVNIVGLLGFCYTSRKAALIYE 400

Query: 121 FMSNGSLESFISRTQNLEMKIVHRIVVGVAKGLEYLHCGCSTRIVHFDIKPQNILLDEDF 180
           FM+NGSL+ F+SR+ N EMK++HRIV GVA+GLEYLHCGCSTRIVHFDIKPQNILLDED 
Sbjct: 401 FMANGSLDRFMSRSHNHEMKMLHRIVTGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDS 460

Query: 181 NTKISDFGLAKLCKRKDSAISMLGSRGTVGFMAPEVYSRAFGIVSYKSDVYSYGMLVLDL 240
           N KISDFGLAKLCKRK SAISMLG+RGT GF+APEV+S AFGIVSYKSDVYSYGMLVLDL
Sbjct: 461 NPKISDFGLAKLCKRKVSAISMLGTRGTAGFIAPEVFSPAFGIVSYKSDVYSYGMLVLDL 520

Query: 241 VLGGRRN--VSSKFLS-DDSEMYFPNWVLKNFEAGKTLRSNQNL-TEEEEEMEKKMVIVG 300
           VLGG RN    S+ LS DDSEMYFPNWV KN E GK++R  Q+L  EEEEEMEKKM ++G
Sbjct: 521 VLGGIRNNPNHSRLLSDDDSEMYFPNWVFKNIEMGKSIRMRQSLMEEEEEEMEKKMTMIG 580

Query: 301 LWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPPRSSLVLPAEMSTQQSVSQSLS 350
           LWCIQTSP+DRP+M++VLEML G+ HSLQMPPR  LV P  M+TQQS S+SLS
Sbjct: 581 LWCIQTSPIDRPTMSRVLEMLEGSIHSLQMPPRPLLVAP-NMATQQSTSESLS 632

BLAST of Spg035556 vs. NCBI nr
Match: XP_011654383.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 [Cucumis sativus])

HSP 1 Score: 502.3 bits (1292), Expect = 3.4e-138
Identity = 260/353 (73.65%), Postives = 303/353 (85.84%), Query Frame = 0

Query: 1   MIIYIRRRRRLSSKTLSTFKKKMLTMELVMNSKPKRYHYSEIKKMTNSFSDKLGEGGYGV 60
           MIIYIRRR+RLS+K+ S   +K   ++ ++N  PKRYHYSEIKKMTNSFS K+GEGGYG+
Sbjct: 1   MIIYIRRRKRLSTKSSSISNRKKYGIDSIINYGPKRYHYSEIKKMTNSFSTKIGEGGYGI 60

Query: 61  VYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMSISKTSHVNIVSLLGVSYNSKEVALVYE 120
           VY+GKL +GTL+AIKVLK+SK++GEDFINEVMSIS+TSHVNIV LLG  Y S++ AL+YE
Sbjct: 61  VYQGKLLDGTLVAIKVLKLSKANGEDFINEVMSISRTSHVNIVGLLGFCYTSRKAALIYE 120

Query: 121 FMSNGSLESFISRTQNLEMKIVHRIVVGVAKGLEYLHCGCSTRIVHFDIKPQNILLDEDF 180
           FM+NGSL+ F+SR+ N EMK++HRIV GVA+GLEYLHCGCSTRIVHFDIKPQNILLDED 
Sbjct: 121 FMANGSLDRFMSRSHNHEMKMLHRIVTGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDS 180

Query: 181 NTKISDFGLAKLCKRKDSAISMLGSRGTVGFMAPEVYSRAFGIVSYKSDVYSYGMLVLDL 240
           N KISDFGLAKLCKRK SAISMLG+RGT GF+APEV+S AFGIVSYKSDVYSYGMLVLDL
Sbjct: 181 NPKISDFGLAKLCKRKVSAISMLGTRGTAGFIAPEVFSPAFGIVSYKSDVYSYGMLVLDL 240

Query: 241 VLGGRRN--VSSKFLS-DDSEMYFPNWVLKNFEAGKTLRSNQNL-TEEEEEMEKKMVIVG 300
           VLGG RN    S+ LS DDSEMYFPNWV KN E  K++R  Q+L  EEEEEMEKKM ++G
Sbjct: 241 VLGGIRNNPNRSRLLSDDDSEMYFPNWVFKNIEMSKSIRMRQSLMEEEEEEMEKKMTMIG 300

Query: 301 LWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPPRSSLVLPAEMSTQQSVSQSLS 350
           LWCIQTSP+DRP+M++VLEML G+ HSLQMPPR  LV P  M+TQQS S+SLS
Sbjct: 301 LWCIQTSPIDRPTMSRVLEMLEGSIHSLQMPPRPLLVAP-NMATQQSTSESLS 352

BLAST of Spg035556 vs. NCBI nr
Match: KAE8649647.1 (hypothetical protein Csa_012280 [Cucumis sativus])

HSP 1 Score: 443.4 bits (1139), Expect = 1.9e-120
Identity = 227/301 (75.42%), Postives = 262/301 (87.04%), Query Frame = 0

Query: 53  LGEGGYGVVYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMSISKTSHVNIVSLLGVSYNS 112
           +GEGGYG+VY+GKL +GTL+AIKVLK+SK++GEDFINEVMSIS+TSHVNIV LLG  Y S
Sbjct: 93  IGEGGYGIVYQGKLLDGTLVAIKVLKLSKANGEDFINEVMSISRTSHVNIVGLLGFCYTS 152

Query: 113 KEVALVYEFMSNGSLESFISRTQNLEMKIVHRIVVGVAKGLEYLHCGCSTRIVHFDIKPQ 172
           ++ AL+YEFM+NGSL+ F+SR+ N EMK++HRIV GVA+GLEYLHCGCSTRIVHFDIKPQ
Sbjct: 153 RKAALIYEFMANGSLDRFMSRSHNHEMKMLHRIVTGVARGLEYLHCGCSTRIVHFDIKPQ 212

Query: 173 NILLDEDFNTKISDFGLAKLCKRKDSAISMLGSRGTVGFMAPEVYSRAFGIVSYKSDVYS 232
           NILLDED N KISDFGLAKLCKRK SAISMLG+RGT GF+APEV+S AFGIVSYKSDVYS
Sbjct: 213 NILLDEDSNPKISDFGLAKLCKRKVSAISMLGTRGTAGFIAPEVFSPAFGIVSYKSDVYS 272

Query: 233 YGMLVLDLVLGGRRN--VSSKFLS-DDSEMYFPNWVLKNFEAGKTLRSNQNL-TEEEEEM 292
           YGMLVLDLVLGG RN    S+ LS DDSEMYFPNWV KN E  K++R  Q+L  EEEEEM
Sbjct: 273 YGMLVLDLVLGGIRNNPNRSRLLSDDDSEMYFPNWVFKNIEMSKSIRMRQSLMEEEEEEM 332

Query: 293 EKKMVIVGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPPRSSLVLPAEMSTQQSVSQSL 350
           EKKM ++GLWCIQTSP+DRP+M++VLEML G+ HSLQMPPR  LV P  M+TQQS S+SL
Sbjct: 333 EKKMTMIGLWCIQTSPIDRPTMSRVLEMLEGSIHSLQMPPRPLLVAP-NMATQQSTSESL 392

BLAST of Spg035556 vs. NCBI nr
Match: XP_021676752.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.1 [Hevea brasiliensis])

HSP 1 Score: 384.0 bits (985), Expect = 1.4e-102
Identity = 189/320 (59.06%), Postives = 244/320 (76.25%), Query Frame = 0

Query: 34  PKRYHYSEIKKMTNSFSDKLGEGGYGVVYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMS 93
           PKRY YSEIKKMT SF+DKLGEGG+G VY+GKL +G   A+K+L  S  DGE+FINEV S
Sbjct: 344 PKRYSYSEIKKMTKSFNDKLGEGGFGGVYKGKLPDGRFAAVKLLNKSMGDGEEFINEVAS 403

Query: 94  ISKTSHVNIVSLLGVSYNSKEVALVYEFMSNGSLESFI------SRTQNLEMKIVHRIVV 153
           IS+TSHVNIV+LLG  Y   + ALVYE+M NGSL+ FI       R  +LE K ++ I +
Sbjct: 404 ISRTSHVNIVTLLGFCYERAKKALVYEYMPNGSLDKFIYNQSSEHRNCHLEWKTLYDIAI 463

Query: 154 GVAKGLEYLHCGCSTRIVHFDIKPQNILLDEDFNTKISDFGLAKLCKRKDSAISMLGSRG 213
           G+A+GLEYLHCGC+TRIVHFDIKPQN+LLD+DF  KISDFGLAKLCK K+S +S+LG++G
Sbjct: 464 GIARGLEYLHCGCNTRIVHFDIKPQNVLLDDDFCPKISDFGLAKLCKGKESRVSILGAKG 523

Query: 214 TVGFMAPEVYSRAFGIVSYKSDVYSYGMLVLDLVLGGRRNVSSKFLSDDSEMYFPNWVLK 273
           T G++APEV+ R++G VSYKSDVYSYGM++L+L  GGR ++++   S  SE+YFP+W+ K
Sbjct: 524 TAGYIAPEVFMRSYGGVSYKSDVYSYGMMILEL-FGGRNDINAGGASQSSELYFPDWIYK 583

Query: 274 NFEAGKTLRSNQNLTEEEEEMEKKMVIVGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMP 333
           + E  +    N+N+T EEEE  ++M+ VGLWCIQT+P DRPSMTKV+EML G   SLQ+P
Sbjct: 584 HIELRQNFVLNENITVEEEETVRRMITVGLWCIQTNPSDRPSMTKVIEMLEGNPQSLQIP 643

Query: 334 PRSSLVLPAEMSTQQSVSQS 348
           P+  L  P+      +VS +
Sbjct: 644 PKPLLFSPSTPPEYSAVSST 662

BLAST of Spg035556 vs. NCBI nr
Match: XP_023894689.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.1 [Quercus suber] >XP_023894690.1 LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.1 [Quercus suber])

HSP 1 Score: 383.3 bits (983), Expect = 2.3e-102
Identity = 194/308 (62.99%), Postives = 240/308 (77.92%), Query Frame = 0

Query: 34  PKRYHYSEIKKMTNSFSDKLGEGGYGVVYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMS 93
           PK Y Y+EIKKMTNSF +KLG+GGY  VY+GKL +G ++A+KVL  SK +GEDFINEV S
Sbjct: 333 PKHYSYAEIKKMTNSFINKLGQGGYSSVYKGKLPDGKIVAVKVLNESKGNGEDFINEVAS 392

Query: 94  ISKTSHVNIVSLLGVSYNSKEVALVYEFMSNGSLESFISRTQN------LEMKIVHRIVV 153
           I +TSHVNIV+LLG SY   + AL+YEFM NGSL+ FI   ++      LE KI+++I V
Sbjct: 393 ICRTSHVNIVTLLGFSYEKNKQALIYEFMPNGSLDKFIYHRESLATNCCLEWKILYKIAV 452

Query: 154 GVAKGLEYLHCGCSTRIVHFDIKPQNILLDEDFNTKISDFGLAKLCKRKDSAISMLGSRG 213
            +A+GLEYLH GCSTRI+HFDIKPQNILLDEDFN KISDFGLAKLC+RKDS +SM+G RG
Sbjct: 453 DIARGLEYLHQGCSTRILHFDIKPQNILLDEDFNPKISDFGLAKLCQRKDSIVSMIGMRG 512

Query: 214 TVGFMAPEVYSRAFGIVSYKSDVYSYGMLVLDLVLGGRRNVSSKFLSDDSEMYFPNWVLK 273
           T G++APEV+SR FG VS+KSDVYSYGMLVL++V GGR+N  +  +S+ SE YFP+W+ K
Sbjct: 513 TAGYIAPEVFSRNFGGVSHKSDVYSYGMLVLEMV-GGRKNFDAG-VSNSSEKYFPDWIYK 572

Query: 274 NFEAGKTLRSNQNLTEEEEEMEKKMVIVGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMP 333
           + E G   R N  +  EE+E   KM++V LWCIQT+P DRPSM KV+EML GT HSLQ+P
Sbjct: 573 DLELGLDGR-NCGVITEEKETSSKMILVSLWCIQTNPTDRPSMNKVVEMLEGTLHSLQIP 632

Query: 334 PRSSLVLP 336
           P+  L  P
Sbjct: 633 PKPFLCSP 637

BLAST of Spg035556 vs. ExPASy Swiss-Prot
Match: Q9FF29 (PR5-like receptor kinase OS=Arabidopsis thaliana OX=3702 GN=PR5K PE=1 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 6.0e-93
Identity = 173/309 (55.99%), Postives = 232/309 (75.08%), Query Frame = 0

Query: 35  KRYHYSEIKKMTNSFSDKLGEGGYGVVYRGKL-DNGTLIAIKVLKVSKSDGEDFINEVMS 94
           KRY Y+ +KKMTNSF+  LG+GG+G VY+GKL D+G  +A+K+LKVS+ +GE+FINEV S
Sbjct: 319 KRYSYTRVKKMTNSFAHVLGKGGFGTVYKGKLADSGRDVAVKILKVSEGNGEEFINEVAS 378

Query: 95  ISKTSHVNIVSLLGVSYNSKEVALVYEFMSNGSLESFISRTQN--LEMKIVHRIVVGVAK 154
           +S+TSHVNIVSLLG  Y   + A++YEFM NGSL+ +IS   +  +E + ++ + VG+++
Sbjct: 379 MSRTSHVNIVSLLGFCYEKNKRAIIYEFMPNGSLDKYISANMSTKMEWERLYDVAVGISR 438

Query: 155 GLEYLHCGCSTRIVHFDIKPQNILLDEDFNTKISDFGLAKLCKRKDSAISMLGSRGTVGF 214
           GLEYLH  C TRIVHFDIKPQNIL+DE+   KISDFGLAKLCK K+S ISML  RGT G+
Sbjct: 439 GLEYLHNRCVTRIVHFDIKPQNILMDENLCPKISDFGLAKLCKNKESIISMLHMRGTFGY 498

Query: 215 MAPEVYSRAFGIVSYKSDVYSYGMLVLDLVLGGRRNVSSKFL--SDDSEMYFPNWVLKNF 274
           +APE++S+ FG VS+KSDVYSYGM+VL+++  G +N+       S++  MYFP WV K+F
Sbjct: 499 IAPEMFSKNFGAVSHKSDVYSYGMVVLEMI--GAKNIEKVEYSGSNNGSMYFPEWVYKDF 558

Query: 275 EAGKTLR-SNQNLTEEEEEMEKKMVIVGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPP 334
           E G+  R    ++T+EEE++ KK+V+V LWCIQ +P DRP M KV+EML G   +LQ+PP
Sbjct: 559 EKGEITRIFGDSITDEEEKIAKKLVLVALWCIQMNPSDRPPMIKVIEMLEGNLEALQVPP 618

Query: 335 RSSLVLPAE 338
              L  P E
Sbjct: 619 NPLLFSPEE 625

BLAST of Spg035556 vs. ExPASy Swiss-Prot
Match: Q9FF31 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.1 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.1 PE=3 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 7.8e-93
Identity = 165/312 (52.88%), Postives = 234/312 (75.00%), Query Frame = 0

Query: 35  KRYHYSEIKKMTNSFSDKLGEGGYGVVYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMSI 94
           K+Y Y+E++K+T  FS  LG+GG+G VY G L +G  +A+K+LK  KS+GEDFINEV S+
Sbjct: 309 KQYSYAEVRKITKLFSHTLGKGGFGTVYGGNLCDGRKVAVKILKDFKSNGEDFINEVASM 368

Query: 95  SKTSHVNIVSLLGVSYNSKEVALVYEFMSNGSLESFIS--RTQNLEMKIVHRIVVGVAKG 154
           S+TSHVNIVSLLG  Y   + A+VYEF+ NGSL+ F+S  ++ NL++  ++RI +GVA+G
Sbjct: 369 SQTSHVNIVSLLGFCYEGSKRAIVYEFLENGSLDQFLSEKKSLNLDVSTLYRIALGVARG 428

Query: 155 LEYLHCGCSTRIVHFDIKPQNILLDEDFNTKISDFGLAKLCKRKDSAISMLGSRGTVGFM 214
           L+YLH GC TRIVHFDIKPQNILLD+ F  K+SDFGLAKLC++++S +S+L +RGT+G++
Sbjct: 429 LDYLHHGCKTRIVHFDIKPQNILLDDTFCPKVSDFGLAKLCEKRESILSLLDARGTIGYI 488

Query: 215 APEVYSRAFGIVSYKSDVYSYGMLVLDLVLGGRRNVSSKFLSDDSEMYFPNWVLKNFEAG 274
           APEV+S  +G VS+KSDVYSYGMLVL+++    + +     S+ S  YFP+W+ KN E G
Sbjct: 489 APEVFSGMYGRVSHKSDVYSYGMLVLEMIGAKNKEIEETAASNSSSAYFPDWIYKNLENG 548

Query: 275 K-TLRSNQNLTEEEEEMEKKMVIVGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPPRSS 334
           + T +    ++ E++E+ KKM +VGLWCIQ SPL+RP M +++EM+ G+   L++PP+ S
Sbjct: 549 EDTWKFGDEISREDKEVAKKMTLVGLWCIQPSPLNRPPMNRIVEMMEGSLDVLEVPPKPS 608

Query: 335 LVLPAEMSTQQS 344
           +   AE   Q S
Sbjct: 609 IHYSAEPLPQLS 620

BLAST of Spg035556 vs. ExPASy Swiss-Prot
Match: F4HQ22 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.4 PE=3 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.6e-90
Identity = 175/358 (48.88%), Postives = 255/358 (71.23%), Query Frame = 0

Query: 1   MIIYIRRRRRLSS-KTLSTFKKKMLTMELVMNSKPKRYHYSEIKKMTNSFSDKLGEGGYG 60
           ++++I R+++ S+ K     K+ +    +    + K+Y Y ++K++TNSF++ +G GG+G
Sbjct: 260 LVVHIIRKQKTSNDKGQQDLKEHIPKPRIKALIQLKQYSYEQVKRITNSFAEVVGRGGFG 319

Query: 61  VVYRGKLDNGTLIAIKVLKVSK-SDGEDFINEVMSISKTSHVNIVSLLGVSYNSKEVALV 120
           +VYRG L +G ++A+KVLK  K ++GEDFINEV S+S+TSHVNIV+LLG      + A++
Sbjct: 320 IVYRGTLSDGRMVAVKVLKDLKGNNGEDFINEVASMSQTSHVNIVTLLGFCSEGYKRAII 379

Query: 121 YEFMSNGSLESFIS--RTQNLEMKIVHRIVVGVAKGLEYLHCGCSTRIVHFDIKPQNILL 180
           YEFM NGSL+ FIS  ++  ++ + ++ I +GVA+GLEYLH GC TRIVHFDIKPQN+LL
Sbjct: 380 YEFMENGSLDKFISSKKSSTMDWRELYGIALGVARGLEYLHHGCRTRIVHFDIKPQNVLL 439

Query: 181 DEDFNTKISDFGLAKLCKRKDSAISMLGSRGTVGFMAPEVYSRAFGIVSYKSDVYSYGML 240
           D++ + K+SDFGLAKLC+RK+S +S++ +RGT+G++APEV+SR +G VS+KSDVYSYGML
Sbjct: 440 DDNLSPKVSDFGLAKLCERKESILSLMDTRGTIGYIAPEVFSRVYGSVSHKSDVYSYGML 499

Query: 241 VLDLVLGGRRNVSSKFLSDDSEMYFPNWVLKNFEAGKTLRSNQNLTEEEEEMEKKMVIVG 300
           VLD++    +  +    S  S MYFP W+ K+ E G   R   N   EE+E+ KKM +VG
Sbjct: 500 VLDIIGARNKTSTEDTTSSTSSMYFPEWIYKDLEKGDNGRLIVN-RSEEDEIAKKMTLVG 559

Query: 301 LWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPPRSSL---VLPAEMSTQQSVSQSLSPE 352
           LWCIQ  PLDRP+M +V+EM+ G   +L++PPR  L   V+P   S+  S   S+S E
Sbjct: 560 LWCIQPWPLDRPAMNRVVEMMEGNLDALEVPPRPVLQCSVVPHLDSSWISEENSISSE 616

BLAST of Spg035556 vs. ExPASy Swiss-Prot
Match: Q9FID5 (Probable receptor-like protein kinase At5g39030 OS=Arabidopsis thaliana OX=3702 GN=At5g39030 PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 6.2e-90
Identity = 166/346 (47.98%), Postives = 251/346 (72.54%), Query Frame = 0

Query: 1   MIIYIRRRRRLSSKTLSTFKKKMLTMELVMNSKPKRYHYSEIKKMTNSFSDKLGEGGYGV 60
           +++++R+ +R + K     +++++  + ++N     Y Y+E+KK+T SFS  +G+GG+G 
Sbjct: 461 LMLWMRQMKRKNRK-----EERVVMFKKLLN----MYTYAELKKITKSFSYIIGKGGFGT 520

Query: 61  VYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMSISKTSHVNIVSLLGVSYNSKEVALVYE 120
           VY G L NG  +A+KVLK  K   EDFINEV S+S+TSHVNIVSLLG  +   + A+VYE
Sbjct: 521 VYGGNLSNGRKVAVKVLKDLKGSAEDFINEVASMSQTSHVNIVSLLGFCFEGSKRAIVYE 580

Query: 121 FMSNGSLESFISRTQNL--EMKIVHRIVVGVAKGLEYLHCGCSTRIVHFDIKPQNILLDE 180
           F+ NGSL+ F+SR ++L  ++  ++ I +G+A+GLEYLH GC TRIVHFDIKPQNILLD 
Sbjct: 581 FLENGSLDQFMSRNKSLTQDVTTLYGIALGIARGLEYLHYGCKTRIVHFDIKPQNILLDG 640

Query: 181 DFNTKISDFGLAKLCKRKDSAISMLGSRGTVGFMAPEVYSRAFGIVSYKSDVYSYGMLVL 240
           +   K+SDFGLAKLC++++S +S++ +RGT+G++APEV+SR +G VS+KSDVYS+GMLV+
Sbjct: 641 NLCPKVSDFGLAKLCEKRESVLSLMDTRGTIGYIAPEVFSRMYGRVSHKSDVYSFGMLVI 700

Query: 241 DLVLGGRRNVSSKFLSDDSEMYFPNWVLKNFEAG-KTLRSNQNLTEEEEEMEKKMVIVGL 300
           D++    + +     S  S  YFP+W+ K+ E G +T      +T+EE+E+ KKM++VGL
Sbjct: 701 DMIGARSKEIVETVDSAASSTYFPDWIYKDLEDGEQTWIFGDEITKEEKEIAKKMIVVGL 760

Query: 301 WCIQTSPLDRPSMTKVLEMLGGTSHSLQMPPRSSLVLPAEMSTQQS 344
           WCIQ  P DRPSM +V+EM+ G+  +L++PP+ S+ +  E+ T+ S
Sbjct: 761 WCIQPCPSDRPSMNRVVEMMEGSLDALEIPPKPSMHISTEVITESS 797

BLAST of Spg035556 vs. ExPASy Swiss-Prot
Match: D7SFH9 (Protein SUPPRESSOR OF NPR1-1 CONSTITUTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.6 PE=1 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.1e-89
Identity = 160/304 (52.63%), Postives = 229/304 (75.33%), Query Frame = 0

Query: 35   KRYHYSEIKKMTNSFSDKLGEGGYGVVYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMSI 94
            + Y Y+++K++T SF++ +G GG+G+VY+G L +G ++A+KVLK +K +GEDFINEV ++
Sbjct: 793  EHYTYAQVKRITKSFAEVVGRGGFGIVYKGTLSDGRVVAVKVLKDTKGNGEDFINEVATM 852

Query: 95   SKTSHVNIVSLLGVSYNSKEVALVYEFMSNGSLESFI--SRTQNLEMKIVHRIVVGVAKG 154
            S+TSH+NIVSLLG      + A++YEF+ NGSL+ FI    + N++   ++RI +GVA G
Sbjct: 853  SRTSHLNIVSLLGFCSEGSKRAIIYEFLENGSLDKFILGKTSVNMDWTALYRIALGVAHG 912

Query: 155  LEYLHCGCSTRIVHFDIKPQNILLDEDFNTKISDFGLAKLCKRKDSAISMLGSRGTVGFM 214
            LEYLH  C TRIVHFDIKPQN+LLD+ F  K+SDFGLAKLC++K+S +SML +RGT+G++
Sbjct: 913  LEYLHHSCKTRIVHFDIKPQNVLLDDSFCPKVSDFGLAKLCEKKESILSMLDTRGTIGYI 972

Query: 215  APEVYSRAFGIVSYKSDVYSYGMLVLDLVLGGRRN---VSSKFLSDDSEMYFPNWVLKNF 274
            APE+ SR +G VS+KSDVYSYGMLVL+++  G RN    +    S+ S MYFP WV ++ 
Sbjct: 973  APEMISRVYGNVSHKSDVYSYGMLVLEII--GARNKEKANQACASNTSSMYFPEWVYRDL 1032

Query: 275  EAGKTLRS-NQNLTEEEEEMEKKMVIVGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPP 333
            E+ K+ R     +  EE+E+ KKM +VGLWCIQ SP+DRP+M +V+EM+ G+  +L++PP
Sbjct: 1033 ESCKSGRHIEDGINSEEDELAKKMTLVGLWCIQPSPVDRPAMNRVVEMMEGSLEALEVPP 1092

BLAST of Spg035556 vs. ExPASy TrEMBL
Match: A0A6M9BN16 (LRK10L2 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 3.4e-139
Identity = 261/353 (73.94%), Postives = 304/353 (86.12%), Query Frame = 0

Query: 1   MIIYIRRRRRLSSKTLSTFKKKMLTMELVMNSKPKRYHYSEIKKMTNSFSDKLGEGGYGV 60
           MIIYIRRR+RLS+K+ S   +K   ++ ++N  PKRYHYSEIKKMTNSFS K+GEGGYG+
Sbjct: 281 MIIYIRRRKRLSTKSSSISNRKKYGIDSIINYGPKRYHYSEIKKMTNSFSTKIGEGGYGI 340

Query: 61  VYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMSISKTSHVNIVSLLGVSYNSKEVALVYE 120
           VY+GKL +GTL+AIKVLK+SK++GEDFINEVMSIS+TSHVNIV LLG  Y S++ AL+YE
Sbjct: 341 VYQGKLLDGTLVAIKVLKLSKANGEDFINEVMSISRTSHVNIVGLLGFCYTSRKAALIYE 400

Query: 121 FMSNGSLESFISRTQNLEMKIVHRIVVGVAKGLEYLHCGCSTRIVHFDIKPQNILLDEDF 180
           FM+NGSL+ F+SR+ N EMK++HRIV GVA+GLEYLHCGCSTRIVHFDIKPQNILLDED 
Sbjct: 401 FMANGSLDRFMSRSHNHEMKMLHRIVTGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDS 460

Query: 181 NTKISDFGLAKLCKRKDSAISMLGSRGTVGFMAPEVYSRAFGIVSYKSDVYSYGMLVLDL 240
           N KISDFGLAKLCKRK SAISMLG+RGT GF+APEV+S AFGIVSYKSDVYSYGMLVLDL
Sbjct: 461 NPKISDFGLAKLCKRKVSAISMLGTRGTAGFIAPEVFSPAFGIVSYKSDVYSYGMLVLDL 520

Query: 241 VLGGRRN--VSSKFLS-DDSEMYFPNWVLKNFEAGKTLRSNQNL-TEEEEEMEKKMVIVG 300
           VLGG RN    S+ LS DDSEMYFPNWV KN E GK++R  Q+L  EEEEEMEKKM ++G
Sbjct: 521 VLGGIRNNPNHSRLLSDDDSEMYFPNWVFKNIEMGKSIRMRQSLMEEEEEEMEKKMTMIG 580

Query: 301 LWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPPRSSLVLPAEMSTQQSVSQSLS 350
           LWCIQTSP+DRP+M++VLEML G+ HSLQMPPR  LV P  M+TQQS S+SLS
Sbjct: 581 LWCIQTSPIDRPTMSRVLEMLEGSIHSLQMPPRPLLVAP-NMATQQSTSESLS 632

BLAST of Spg035556 vs. ExPASy TrEMBL
Match: A0A5C7INK8 (Protein kinase domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_004981 PE=4 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 3.3e-102
Identity = 194/321 (60.44%), Postives = 244/321 (76.01%), Query Frame = 0

Query: 35  KRYHYSEIKKMTNSFSDKLGEGGYGVVYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMSI 94
           +R+ YS +KKMT SFS KLG+GGYG VY+GKL +G L A+KVLK SK +GE+FINEV SI
Sbjct: 267 QRFDYSFVKKMTKSFSTKLGQGGYGDVYKGKLPDGRLAAVKVLKESKGNGEEFINEVASI 326

Query: 95  SKTSHVNIVSLLGVSYNSKEVALVYEFMSNGSLESFI------SRTQNLEMKIVHRIVVG 154
            +TSHVN+V+LLG  YN  + AL+YEFMSNGSL+ +I      S   NLE K  ++I +G
Sbjct: 327 CRTSHVNVVALLGFCYNKNKRALIYEFMSNGSLDKYIFDKESSSTKCNLEWKTRYQIAIG 386

Query: 155 VAKGLEYLHCGCSTRIVHFDIKPQNILLDEDFNTKISDFGLAKLCKRKDSAISMLGSRGT 214
           +A+GLEYLH GC+TRIVHFDIKP NILLDEDF  KISDFGLAKLCK K+S ISMLG+RGT
Sbjct: 387 IARGLEYLHRGCNTRIVHFDIKPHNILLDEDFCPKISDFGLAKLCKNKESIISMLGARGT 446

Query: 215 VGFMAPEVYSRAFGIVSYKSDVYSYGMLVLDLVLGGRRNVSSKFLSDDSEMYFPNWVLKN 274
           VG++APEV+ R+FG VS+KSD+YSYGM++L++V GGR N+    +S  SE+YFPNW+ K 
Sbjct: 447 VGYIAPEVFCRSFGEVSHKSDIYSYGMMILEMV-GGRNNIVVS-VSHTSEIYFPNWIYKQ 506

Query: 275 FEAGKTLRSNQNLTEEEEEMEKKMVIVGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPP 334
            E GK    +  +TEEE+E+ KKM+IV +WCIQT P DRPSM KV+EML G+  SL++PP
Sbjct: 507 LEPGKDFEFSGAVTEEEKEVVKKMIIVSMWCIQTIPSDRPSMNKVVEMLEGSLESLEIPP 566

Query: 335 RSSLVLPAEMSTQQSVSQSLS 350
           + SL LP       + S S S
Sbjct: 567 KPSLSLPTRSPKHSAPSSSTS 585

BLAST of Spg035556 vs. ExPASy TrEMBL
Match: A0A2N9I4J7 (Protein kinase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS48869 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.6e-101
Identity = 205/355 (57.75%), Postives = 259/355 (72.96%), Query Frame = 0

Query: 7    RRRRLSSKTL---STFKKKMLTMELVMNS---KPKRYHYSEIKKMTNSFSDKLGEGGYGV 66
            RR +LSS  +      K        +MN     PKRY YSEIKKMTNSF +KLG+GGYG 
Sbjct: 1430 RREQLSSMVVMKNKNMKDDQYVKSFIMNHGSFAPKRYTYSEIKKMTNSFKEKLGQGGYGS 1489

Query: 67   VYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMSISKTSHVNIVSLLGVSYNSKEVALVYE 126
            VY+GKL +G L+A+KV+   K  GE+FINEV SIS+TSHVNIV+LLG SY + + ALVYE
Sbjct: 1490 VYKGKLLDGRLVAVKVINELKGSGEEFINEVASISRTSHVNIVTLLGYSYETTKRALVYE 1549

Query: 127  FMSNGSLESFI------SRTQNLEMKIVHRIVVGVAKGLEYLHCGCSTRIVHFDIKPQNI 186
            FM NGSL+ FI      S    LE + + +IVVG+A+GLEYLH GCSTRI+HFDIKPQNI
Sbjct: 1550 FMPNGSLDKFIYNHEPSSTNCQLEREKLFQIVVGIARGLEYLHRGCSTRILHFDIKPQNI 1609

Query: 187  LLDEDFNTKISDFGLAKLCKRKDSAISMLGSRGTVGFMAPEVYSRAFGIVSYKSDVYSYG 246
            LLDEDF  KISDFGLA+LC++K+S ISMLG+RGT+G++APEV+SR FG VSYK+DVYSYG
Sbjct: 1610 LLDEDFCPKISDFGLARLCQKKESIISMLGTRGTIGYIAPEVFSRNFGGVSYKADVYSYG 1669

Query: 247  MLVLDLVLGGRRNVSSKFLSDDSEMYFPNWVLKNFEAGKTLRSNQNLTEEEEEMEKKMVI 306
            MLVL++V  GRR  S+  +S  SE YFP+W+ +       + ++ ++ EE EEME+KM++
Sbjct: 1670 MLVLEMV--GRRKNSNVVMSHSSENYFPHWIYQELNLNVNVGNHMDMMEEGEEMERKMIL 1729

Query: 307  VGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPPRSSLVLPAEMSTQQSVSQSLS 350
            V LWCIQT P DRPSMTKV+EML G   S+++PP+  L  P + S Q S + SLS
Sbjct: 1730 VSLWCIQTVPADRPSMTKVVEMLEGGLQSIEIPPKPFLSSP-KRSIQNSSTTSLS 1781

BLAST of Spg035556 vs. ExPASy TrEMBL
Match: A0A2N9FST5 (Protein kinase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS18087 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.6e-101
Identity = 196/322 (60.87%), Postives = 248/322 (77.02%), Query Frame = 0

Query: 34  PKRYHYSEIKKMTNSFSDKLGEGGYGVVYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMS 93
           PKRY YSEIKKMTNSF +KLG+GGYG VY+GKL +G L+A+KV+   K  GE+FINEV S
Sbjct: 27  PKRYTYSEIKKMTNSFKEKLGQGGYGSVYKGKLLDGRLVAVKVINELKGSGEEFINEVAS 86

Query: 94  ISKTSHVNIVSLLGVSYNSKEVALVYEFMSNGSLESFI------SRTQNLEMKIVHRIVV 153
           IS+TSHVNIV+LLG SY + + ALVYEFM NGSL+ FI      S    LE + + +IVV
Sbjct: 87  ISRTSHVNIVTLLGYSYETTKRALVYEFMPNGSLDKFIYNHEPSSTNCQLEREKLFQIVV 146

Query: 154 GVAKGLEYLHCGCSTRIVHFDIKPQNILLDEDFNTKISDFGLAKLCKRKDSAISMLGSRG 213
           G+A+GLEYLH GCSTRI+HFDIKPQNILLDEDF  KISDFGLA+LC++K+S +SMLG+RG
Sbjct: 147 GIARGLEYLHRGCSTRILHFDIKPQNILLDEDFCPKISDFGLARLCQKKESIVSMLGTRG 206

Query: 214 TVGFMAPEVYSRAFGIVSYKSDVYSYGMLVLDLVLGGRRNVSSKFLSDDSEMYFPNWVLK 273
           T+G++APEV+SR FG VSYK+DVYSYGMLVL++V  GRR  S+  +S  SE YFP+W+ +
Sbjct: 207 TIGYIAPEVFSRNFGGVSYKADVYSYGMLVLEMV--GRRKNSNVVMSHSSENYFPHWIYQ 266

Query: 274 NFEAGKTLRSNQNLTEEEEEMEKKMVIVGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMP 333
                  + ++ ++ EE EEME+KM++V LWCIQT P DRPSMTKV+EML G   S+++P
Sbjct: 267 ELNLNVNVGNHMDMMEEGEEMERKMILVSLWCIQTVPPDRPSMTKVVEMLEGGLQSIEIP 326

Query: 334 PRSSLVLPAEMSTQQSVSQSLS 350
           P+  L  P + S Q S + SLS
Sbjct: 327 PKPFLSSP-KRSIQNSSTTSLS 345

BLAST of Spg035556 vs. ExPASy TrEMBL
Match: A0A1R3ICL4 (Protein kinase domain-containing protein OS=Corchorus capsularis OX=210143 GN=CCACVL1_13091 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 6.2e-101
Identity = 188/328 (57.32%), Postives = 249/328 (75.91%), Query Frame = 0

Query: 26  MELVMNSKPKRYHYSEIKKMTNSFSDKLGEGGYGVVYRGKLDNGTLIAIKVLKVSKSDGE 85
           ME   +  PKRY +S++KKMT+SF DKLG+GGYG VY+GKL NG L+A+KVL  SK +GE
Sbjct: 284 MENYGSLAPKRYSHSDVKKMTSSFKDKLGQGGYGSVYKGKLPNGQLVAVKVLNESKGNGE 343

Query: 86  DFINEVMSISKTSHVNIVSLLGVSYNSKEVALVYEFMSNGSLESFI------SRTQNLEM 145
           +FINEV SIS+TSHVN+V+LLG  Y S + AL+YEFM NGSL+ FI      + + NLE 
Sbjct: 344 EFINEVASISRTSHVNVVTLLGFCYESSKRALIYEFMPNGSLDRFIYDKKHQNTSTNLEW 403

Query: 146 KIVHRIVVGVAKGLEYLHCGCSTRIVHFDIKPQNILLDEDFNTKISDFGLAKLCKRKDSA 205
           + ++ I +G+A+GLEYLH GC+TRI+HFDIKP NILLDE F  +ISDFGLAKLC+RK+S 
Sbjct: 404 RTLYEITLGIARGLEYLHQGCNTRILHFDIKPHNILLDEQFRPRISDFGLAKLCQRKESI 463

Query: 206 ISMLGSRGTVGFMAPEVYSRAFGIVSYKSDVYSYGMLVLDLVLGGRRNVSSKFLSDDSEM 265
           +SMLG+RGT G+MAPEVY+R+FG VS+KSDVYS+GM+VL++  GGRRN+    +S  SE+
Sbjct: 464 VSMLGARGTAGYMAPEVYNRSFGEVSHKSDVYSFGMMVLEMA-GGRRNIDVD-ISHTSEI 523

Query: 266 YFPNWVLKNFEAGKTLRSNQNLTEEEEEMEKKMVIVGLWCIQTSPLDRPSMTKVLEMLGG 325
           YFP+W+ K  E+G+ L+ ++ +TE++E++ +KM++V LWCIQ  P DRPSM KV+EML G
Sbjct: 524 YFPHWIYKQLESGQDLKLHRVVTEDDEKLARKMILVSLWCIQIKPSDRPSMKKVVEMLEG 583

Query: 326 TSHSLQMPPRSSLVLPAEMSTQQSVSQS 348
           +  SL  PP+  L  P       S S S
Sbjct: 584 SLESLPTPPQPYLSSPVREQLLSSPSSS 609

BLAST of Spg035556 vs. TAIR 10
Match: AT5G38280.1 (PR5-like receptor kinase )

HSP 1 Score: 342.4 bits (877), Expect = 4.3e-94
Identity = 173/309 (55.99%), Postives = 232/309 (75.08%), Query Frame = 0

Query: 35  KRYHYSEIKKMTNSFSDKLGEGGYGVVYRGKL-DNGTLIAIKVLKVSKSDGEDFINEVMS 94
           KRY Y+ +KKMTNSF+  LG+GG+G VY+GKL D+G  +A+K+LKVS+ +GE+FINEV S
Sbjct: 319 KRYSYTRVKKMTNSFAHVLGKGGFGTVYKGKLADSGRDVAVKILKVSEGNGEEFINEVAS 378

Query: 95  ISKTSHVNIVSLLGVSYNSKEVALVYEFMSNGSLESFISRTQN--LEMKIVHRIVVGVAK 154
           +S+TSHVNIVSLLG  Y   + A++YEFM NGSL+ +IS   +  +E + ++ + VG+++
Sbjct: 379 MSRTSHVNIVSLLGFCYEKNKRAIIYEFMPNGSLDKYISANMSTKMEWERLYDVAVGISR 438

Query: 155 GLEYLHCGCSTRIVHFDIKPQNILLDEDFNTKISDFGLAKLCKRKDSAISMLGSRGTVGF 214
           GLEYLH  C TRIVHFDIKPQNIL+DE+   KISDFGLAKLCK K+S ISML  RGT G+
Sbjct: 439 GLEYLHNRCVTRIVHFDIKPQNILMDENLCPKISDFGLAKLCKNKESIISMLHMRGTFGY 498

Query: 215 MAPEVYSRAFGIVSYKSDVYSYGMLVLDLVLGGRRNVSSKFL--SDDSEMYFPNWVLKNF 274
           +APE++S+ FG VS+KSDVYSYGM+VL+++  G +N+       S++  MYFP WV K+F
Sbjct: 499 IAPEMFSKNFGAVSHKSDVYSYGMVVLEMI--GAKNIEKVEYSGSNNGSMYFPEWVYKDF 558

Query: 275 EAGKTLR-SNQNLTEEEEEMEKKMVIVGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPP 334
           E G+  R    ++T+EEE++ KK+V+V LWCIQ +P DRP M KV+EML G   +LQ+PP
Sbjct: 559 EKGEITRIFGDSITDEEEKIAKKLVLVALWCIQMNPSDRPPMIKVIEMLEGNLEALQVPP 618

Query: 335 RSSLVLPAE 338
              L  P E
Sbjct: 619 NPLLFSPEE 625

BLAST of Spg035556 vs. TAIR 10
Match: AT5G38260.1 (Protein kinase superfamily protein )

HSP 1 Score: 342.0 bits (876), Expect = 5.6e-94
Identity = 165/312 (52.88%), Postives = 234/312 (75.00%), Query Frame = 0

Query: 35  KRYHYSEIKKMTNSFSDKLGEGGYGVVYRGKLDNGTLIAIKVLKVSKSDGEDFINEVMSI 94
           K+Y Y+E++K+T  FS  LG+GG+G VY G L +G  +A+K+LK  KS+GEDFINEV S+
Sbjct: 309 KQYSYAEVRKITKLFSHTLGKGGFGTVYGGNLCDGRKVAVKILKDFKSNGEDFINEVASM 368

Query: 95  SKTSHVNIVSLLGVSYNSKEVALVYEFMSNGSLESFIS--RTQNLEMKIVHRIVVGVAKG 154
           S+TSHVNIVSLLG  Y   + A+VYEF+ NGSL+ F+S  ++ NL++  ++RI +GVA+G
Sbjct: 369 SQTSHVNIVSLLGFCYEGSKRAIVYEFLENGSLDQFLSEKKSLNLDVSTLYRIALGVARG 428

Query: 155 LEYLHCGCSTRIVHFDIKPQNILLDEDFNTKISDFGLAKLCKRKDSAISMLGSRGTVGFM 214
           L+YLH GC TRIVHFDIKPQNILLD+ F  K+SDFGLAKLC++++S +S+L +RGT+G++
Sbjct: 429 LDYLHHGCKTRIVHFDIKPQNILLDDTFCPKVSDFGLAKLCEKRESILSLLDARGTIGYI 488

Query: 215 APEVYSRAFGIVSYKSDVYSYGMLVLDLVLGGRRNVSSKFLSDDSEMYFPNWVLKNFEAG 274
           APEV+S  +G VS+KSDVYSYGMLVL+++    + +     S+ S  YFP+W+ KN E G
Sbjct: 489 APEVFSGMYGRVSHKSDVYSYGMLVLEMIGAKNKEIEETAASNSSSAYFPDWIYKNLENG 548

Query: 275 K-TLRSNQNLTEEEEEMEKKMVIVGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPPRSS 334
           + T +    ++ E++E+ KKM +VGLWCIQ SPL+RP M +++EM+ G+   L++PP+ S
Sbjct: 549 EDTWKFGDEISREDKEVAKKMTLVGLWCIQPSPLNRPPMNRIVEMMEGSLDVLEVPPKPS 608

Query: 335 LVLPAEMSTQQS 344
           +   AE   Q S
Sbjct: 609 IHYSAEPLPQLS 620

BLAST of Spg035556 vs. TAIR 10
Match: AT1G70250.1 (receptor serine/threonine kinase, putative )

HSP 1 Score: 341.3 bits (874), Expect = 9.5e-94
Identity = 179/343 (52.19%), Postives = 250/343 (72.89%), Query Frame = 0

Query: 1   MIIYIRRRRRLSSKTLSTFKKKMLTMELVMNSKPKRYHYSEIKKMTNSFSDKLGEGGYGV 60
           +II I  + R ++   S   +K +   +++    KR+ Y ++KKMT SF + LG+GG+G 
Sbjct: 417 IIIVIVGKVRANNMRKSDLNEKNMEAVVML----KRFSYVQVKKMTKSFENVLGKGGFGT 476

Query: 61  VYRGKLDNGTL-IAIKVLKVSKSDGEDFINEVMSISKTSHVNIVSLLGVSYNSKEVALVY 120
           VY+GKL +G+  +A+K+LK S  DGEDFINE+ S+S+TSH NIVSLLG  Y  ++ A++Y
Sbjct: 477 VYKGKLPDGSRDVAVKILKESNEDGEDFINEIASMSRTSHANIVSLLGFCYEGRKKAIIY 536

Query: 121 EFMSNGSLESFISRTQN--LEMKIVHRIVVGVAKGLEYLHCGCSTRIVHFDIKPQNILLD 180
           E M NGSL+ FIS+  +  +E K ++ I VGV+ GLEYLH  C +RIVHFDIKPQNIL+D
Sbjct: 537 ELMPNGSLDKFISKNMSAKMEWKTLYNIAVGVSHGLEYLHSHCVSRIVHFDIKPQNILID 596

Query: 181 EDFNTKISDFGLAKLCKRKDSAISMLGSRGTVGFMAPEVYSRAFGIVSYKSDVYSYGMLV 240
            D   KISDFGLAKLCK  +S ISML +RGT+G++APEV+S+ FG VS+KSDVYSYGM+V
Sbjct: 597 GDLCPKISDFGLAKLCKNNESIISMLHARGTIGYIAPEVFSQNFGGVSHKSDVYSYGMVV 656

Query: 241 LDLVLGGRRNV--SSKFLSDDSEMYFPNWVLKNFEAGKTLR--SNQNLTEEEEEMEKKMV 300
           L+++  G RN+  +    S ++ MYFP+W+ K+ E G+ +   ++Q   EE+E++ KKMV
Sbjct: 657 LEMI--GARNIGRAQNAGSSNTSMYFPDWIYKDLEKGEIMSFLADQITEEEDEKIVKKMV 716

Query: 301 IVGLWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPPRSSLVLPA 337
           +VGLWCIQT+P DRP M+KV+EML G+  +LQ+PP+  L LPA
Sbjct: 717 LVGLWCIQTNPYDRPPMSKVVEMLEGSLEALQIPPKPLLCLPA 753

BLAST of Spg035556 vs. TAIR 10
Match: AT4G18250.1 (receptor serine/threonine kinase, putative )

HSP 1 Score: 335.1 bits (858), Expect = 6.8e-92
Identity = 170/308 (55.19%), Postives = 231/308 (75.00%), Query Frame = 0

Query: 35  KRYHYSEIKKMTNSFSDKLGEGGYGVVYRGKLD--NGTLIAIKVLKVSKSDGEDFINEVM 94
           KRY + ++KKMTNSF   +G+GG+G VY+GKL   +G  IA+K+LK SK +GE+FINE++
Sbjct: 507 KRYSFEKVKKMTNSFDHVIGKGGFGTVYKGKLPDASGRDIALKILKESKGNGEEFINELV 566

Query: 95  SISKTSHVNIVSLLGVSYNSKEVALVYEFMSNGSLESFISRTQN--LEMKIVHRIVVGVA 154
           S+S+ SHVNIVSL G  Y   + A++YEFM NGSL+ FIS   +  +E K ++ I VGVA
Sbjct: 567 SMSRASHVNIVSLFGFCYEGSQRAIIYEFMPNGSLDKFISENMSTKIEWKTLYNIAVGVA 626

Query: 155 KGLEYLHCGCSTRIVHFDIKPQNILLDEDFNTKISDFGLAKLCKRKDSAISMLGSRGTVG 214
           +GLEYLH  C ++IVHFDIKPQNIL+DED   KISDFGLAKLCK+K+S ISML +RGTVG
Sbjct: 627 RGLEYLHNSCVSKIVHFDIKPQNILIDEDLCPKISDFGLAKLCKKKESIISMLDARGTVG 686

Query: 215 FMAPEVYSRAFGIVSYKSDVYSYGMLVLDLVLGGRRNVSSKFLSDDSEMYFPNWVLKNFE 274
           ++APE++S+ +G VS+KSDVYSYGM+VL+++   +R       +D S MYFP+WV ++ E
Sbjct: 687 YIAPEMFSKNYGGVSHKSDVYSYGMVVLEMIGATKREEVETSATDKSSMYFPDWVYEDLE 746

Query: 275 AGKTLRSNQN---LTEEEEEMEKKMVIVGLWCIQTSPLDRPSMTKVLEMLGGTS-HSLQM 334
             +T+R  ++     EEEE++ K+M +VGLWCIQT+P DRP M KV+EML G+   +LQ+
Sbjct: 747 RKETMRLLEDHIIEEEEEEKIVKRMTLVGLWCIQTNPSDRPPMRKVVEMLEGSRLEALQV 806

BLAST of Spg035556 vs. TAIR 10
Match: AT1G66920.1 (Protein kinase superfamily protein )

HSP 1 Score: 334.3 bits (856), Expect = 1.2e-91
Identity = 175/358 (48.88%), Postives = 255/358 (71.23%), Query Frame = 0

Query: 1   MIIYIRRRRRLSS-KTLSTFKKKMLTMELVMNSKPKRYHYSEIKKMTNSFSDKLGEGGYG 60
           ++++I R+++ S+ K     K+ +    +    + K+Y Y ++K++TNSF++ +G GG+G
Sbjct: 252 LVVHIIRKQKTSNDKGQQDLKEHIPKPRIKALIQLKQYSYEQVKRITNSFAEVVGRGGFG 311

Query: 61  VVYRGKLDNGTLIAIKVLKVSK-SDGEDFINEVMSISKTSHVNIVSLLGVSYNSKEVALV 120
           +VYRG L +G ++A+KVLK  K ++GEDFINEV S+S+TSHVNIV+LLG      + A++
Sbjct: 312 IVYRGTLSDGRMVAVKVLKDLKGNNGEDFINEVASMSQTSHVNIVTLLGFCSEGYKRAII 371

Query: 121 YEFMSNGSLESFIS--RTQNLEMKIVHRIVVGVAKGLEYLHCGCSTRIVHFDIKPQNILL 180
           YEFM NGSL+ FIS  ++  ++ + ++ I +GVA+GLEYLH GC TRIVHFDIKPQN+LL
Sbjct: 372 YEFMENGSLDKFISSKKSSTMDWRELYGIALGVARGLEYLHHGCRTRIVHFDIKPQNVLL 431

Query: 181 DEDFNTKISDFGLAKLCKRKDSAISMLGSRGTVGFMAPEVYSRAFGIVSYKSDVYSYGML 240
           D++ + K+SDFGLAKLC+RK+S +S++ +RGT+G++APEV+SR +G VS+KSDVYSYGML
Sbjct: 432 DDNLSPKVSDFGLAKLCERKESILSLMDTRGTIGYIAPEVFSRVYGSVSHKSDVYSYGML 491

Query: 241 VLDLVLGGRRNVSSKFLSDDSEMYFPNWVLKNFEAGKTLRSNQNLTEEEEEMEKKMVIVG 300
           VLD++    +  +    S  S MYFP W+ K+ E G   R   N   EE+E+ KKM +VG
Sbjct: 492 VLDIIGARNKTSTEDTTSSTSSMYFPEWIYKDLEKGDNGRLIVN-RSEEDEIAKKMTLVG 551

Query: 301 LWCIQTSPLDRPSMTKVLEMLGGTSHSLQMPPRSSL---VLPAEMSTQQSVSQSLSPE 352
           LWCIQ  PLDRP+M +V+EM+ G   +L++PPR  L   V+P   S+  S   S+S E
Sbjct: 552 LWCIQPWPLDRPAMNRVVEMMEGNLDALEVPPRPVLQCSVVPHLDSSWISEENSISSE 608

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
QKK82668.16.9e-13973.94LRK10L2 [Cucumis sativus][more]
XP_011654383.13.4e-13873.65LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 [Cuc... [more]
KAE8649647.11.9e-12075.42hypothetical protein Csa_012280 [Cucumis sativus][more]
XP_021676752.11.4e-10259.06LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.1 [Hev... [more]
XP_023894689.12.3e-10262.99LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.1 [Que... [more]
Match NameE-valueIdentityDescription
Q9FF296.0e-9355.99PR5-like receptor kinase OS=Arabidopsis thaliana OX=3702 GN=PR5K PE=1 SV=1[more]
Q9FF317.8e-9352.88LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.1 OS=A... [more]
F4HQ221.6e-9048.88LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 OS=A... [more]
Q9FID56.2e-9047.98Probable receptor-like protein kinase At5g39030 OS=Arabidopsis thaliana OX=3702 ... [more]
D7SFH91.1e-8952.63Protein SUPPRESSOR OF NPR1-1 CONSTITUTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=L... [more]
Match NameE-valueIdentityDescription
A0A6M9BN163.4e-13973.94LRK10L2 OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
A0A5C7INK83.3e-10260.44Protein kinase domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_... [more]
A0A2N9I4J71.6e-10157.75Protein kinase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCU... [more]
A0A2N9FST51.6e-10160.87Protein kinase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCU... [more]
A0A1R3ICL46.2e-10157.32Protein kinase domain-containing protein OS=Corchorus capsularis OX=210143 GN=CC... [more]
Match NameE-valueIdentityDescription
AT5G38280.14.3e-9455.99PR5-like receptor kinase [more]
AT5G38260.15.6e-9452.88Protein kinase superfamily protein [more]
AT1G70250.19.5e-9452.19receptor serine/threonine kinase, putative [more]
AT4G18250.16.8e-9255.19receptor serine/threonine kinase, putative [more]
AT1G66920.11.2e-9148.88Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 47..317
e-value: 1.3E-33
score: 127.7
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 50..315
e-value: 8.1E-50
score: 169.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 47..325
score: 38.237431
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 9..121
e-value: 1.6E-26
score: 94.3
NoneNo IPR availablePIRSFPIRSF000654ILKcoord: 8..328
e-value: 1.1E-35
score: 121.2
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 122..326
e-value: 8.0E-49
score: 167.9
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 53..241
e-value: 3.5E-26
score: 90.2
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 41..318
e-value: 1.2E-19
score: 68.3
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 38..243
e-value: 3.7E-25
score: 86.3
NoneNo IPR availablePIRSRPIRSR000556-1PIRSR000556-1coord: 38..243
e-value: 3.7E-25
score: 86.3
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 53..241
e-value: 3.5E-26
score: 90.2
NoneNo IPR availablePANTHERPTHR27009:SF223SUBFAMILY NOT NAMEDcoord: 25..349
NoneNo IPR availablePANTHERPTHR27009RUST RESISTANCE KINASE LR10-RELATEDcoord: 25..349
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 164..176
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 53..75
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 30..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg035556.1Spg035556.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004672 protein kinase activity