Spg035470 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg035470
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase domain-containing protein
Locationscaffold3: 2918101 .. 2921076 (-)
RNA-Seq ExpressionSpg035470
SyntenySpg035470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGCAAACCCGAGACTTTCCTTGGATCATTTACTCTTGTCATCTATTGTGGTTTCATTGTTGAATTTAAGTTGTTGGGATTTCCTAATTTAAAAGAAGAAAGGGAGTGAAGAAAAGAATGGAAATAAGGAAGAAAAGAAAAGAAAAGAAAAGAAAAGGAAAAGGAAAAGGGAATAAGGAAAAAAAAAAAAGAAAGAAAATGGGAAAACCACTTTCGGTGCTTAGAAACCCACCACCGACTAGCATTCTTATCAGCCCATTTTCAGCCCAATTTCTCATCATTTTCCATCCAAAGATTCAGAGCACTTGAGGGAGAGAGTGAAGGAAGAATAAGAAGAAGAATTTCGTGGTTTTGAGGTTGAAGAAAATAGGAAAAGCTCATGCAAAGCCAGCCATAGCAGAATCTGAGTTCAACCTGTCAACCTCTGCCTTTTTATTCGGTTTGAGCTACACAGAAGGAATTAAGAGGTGAGGTTTAATTTTCATGAAAGTTAATCCATTTTCTAACCAATTTCATGAAGGAAGCTTGAGCTAAATCTGATGAGAAGTTTATGGTTTTTGAGATGGAAAACAGAGCACAGGATTTTCGTGCAAACCAAGAGGATCTCTAGTGTTCTCTGGTGTTTTGAGCATTCTGGGGTGTACAAGTTGAATCAGAAGCTCTATTTGGTATGGTTGGAAAGCTTATTCAATTTCCTACAACTTTCATGAAGAGGTCGTGAGCCAAAAATGACTTGAAGTAGGTTTAATTTCAGGAGATAGATAAAGGGCAGCAGAGAACCTCGAATCTGTGTTGTCTGTGTTCGGGGCGTTTCTGGACAAATTGGTTGAGAATCTCGTTTTCATTTATTCAGGTACGTTGTAGAGTGTTCGCAAATGAAGAGTTTGATGTATAGTACATTAATATTGGTTAAGTATTGAGGAAGTTATGGGTATTGGAAGTTAGGGTATAGAATCTGGAAAATCTGGAAACTAGAATGTGGATGTGATTCTTAATTCAATTGTTTAGAAATTCATGAGCATGGAAAGACAAAACTAATTATGGTTCTGATGCTCGGTTTTGGTTGGTTGACAGGCCAAGAGGAAATAGGTCGGGTCTATAAACCAGGGAACTAGCTAAGACGGTGAGTGACAAAATAAATTTCGAAAATTGTTTCCAACGGTTGTTCATGTTTAGATGTTTTACATGCTTTTCTATAAATGATACATGGTTTTTATGAATGGTTTCAAGCTTAAGTTATGAGCTTAGATTTTCTATTGAGATTTATAAATGAGCACGTGATTATTGAGTTTGAGAAAAATAGTTGAAACAACATCCTTTTAAGTCAAGCATGTATTAGCAAGAATTATTTAAAGATTTTCGGTTTTTCCACGAGCAAGCTGAATAAGCTTGAGTTTTACGAGAGATAACGATAAGCAGGCATGTTCTAATGTTTTCATGGGATTTTCTGAGATTTCTATTGACTACATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCTGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCCCACTACGACAAAGACGATGGGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCGTGCCCTACACAACGTAGATCGGTCATGTTAGTTAAAACGTTTGATGTACTTGATATGTCTTGCTAGATTTTTAATGACGAGCCTGCGATGTTTTTAAGAAAGCTTTTATCGTTGCACGTTTATGTTTAACGCCTTGGTCGAGTATCTTTATACGTTTAAAGTTTTATCACGTGTTCTGATTTCCGAATGTACAGTTTTAAAGTTAGTCACTCACTGGGCTTCGTAGCTCATTCTTTCAAAATGTTTTTCCACTTTCAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCAGATCAAGCTCCAGTACGTGGGTGGAGTTGTATATTGAGTCCGTCTATATTGTAAATGTTTTGTAAGGATTGGACAATTTAGTCCTAGAGGTCGTGGTTGTATAAACCCCATTTGTATATGTTTTTTGTTGTGAAATCTATATCAGGTTATTTTCCAAATCGAGTTATGTCAGGGCTGCACCTCGTGTATATTAGAAGGTTTTCAGGGATTCCGCTGTGTTGTGTTTCATGTGGAATGTTTTATATCTAAGATTAGTATGTTTATCAGGTTCTACGTTTCAGGAGGGTCAACGGGTATCGTTAGAGGAGGACGATATTCGTTGGCTTCACGCCATCTTCCGGACTAAGCTAGCAGGTGGTCTGGGAGGGGGTGTGACAATGACTGTTATTTTTGGGCGCCCTTTTTTCCTTCTTGGACTTTCCCTCTCTTTAATGAGGGTAGGTGTAATGTGGTTGGTCTTTAATTAATCTAATTTCTTTTCAAAAAAAAAAAGAACGGAAAAGTGAAAATATTGGCAAAAGACACTAGTGTCCATAGATATTCTTGAAATTAATCCTTAAATGTTTTTTAAAAAGTTATAAAGTTTGTTGTATTTTGCCAATCTAAACTGTTGATCATGGTCGGAGTTAAAACATGTGCATGTTGTGAGTGATTTTGCGGAAGAGATTGACGATCTTAGGCACCGATTTTCATTAATTTCTCAAGAACAAGCATGCTGAAATTTTCAAATAAAAACAGAGGATTGAAAAACTTAACTTTGAAGCTTCAATCCGTGAGTTCCTTTGGTTGTTTCTTCACACGATCTTCACGTCGACTACCGCTAGAGCCTTCCCCGCTATTCTCCGGCCTTAGAACGGGCTTGTGGGAACCCGAGCATGATGAATTTGATGAAGAACACATGGGTTTTGTGCCTTGGGAGCTATGGTGTTTGAGAGAGTTTTCTTTTCAGTTTTGCATGAGATTTCTCCAGCAACTTCAACTAAATTCCATACCCCTT

mRNA sequence

ATGACTGCAAACCCGAGACTTTCCTTGGATCATTTACTCTTGTCATCTATTGTGGTTTCATTGTTGAATTTAACAGAGAACCTCGAATCTGTGTTGTCTGTGTTCGGGGCGTTTCTGGACAAATTGGTTGAGAATCTCGTTTTCATTTATTCAGATAACGATAAGCAGGCATGTTCTAATGTTTTCATGGGATTTTCTGAGATTTCTATTGACTACATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCAGATCAAGCTCCAGTTCTACGTTTCAGGAGGGTCAACGGGTATCGTTAGAGGAGGACGATATTCGTTGGCTTCACGCCATCTTCCGGACTAAGCTAGCAGGTGGTCTGGGAGGGGAGCCTTCCCCGCTATTCTCCGGCCTTAGAACGGGCTTGTGGGAACCCGAGCATGATGAATTTGATGAAGAACACATGGGTTTTGTGCCTTGGGAGCTATGGTGTTTGAGAGAGTTTTCTTTTCAGTTTTGCATGAGATTTCTCCAGCAACTTCAACTAAATTCCATACCCCTT

Coding sequence (CDS)

ATGACTGCAAACCCGAGACTTTCCTTGGATCATTTACTCTTGTCATCTATTGTGGTTTCATTGTTGAATTTAACAGAGAACCTCGAATCTGTGTTGTCTGTGTTCGGGGCGTTTCTGGACAAATTGGTTGAGAATCTCGTTTTCATTTATTCAGATAACGATAAGCAGGCATGTTCTAATGTTTTCATGGGATTTTCTGAGATTTCTATTGACTACATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCAGATCAAGCTCCAGTTCTACGTTTCAGGAGGGTCAACGGGTATCGTTAGAGGAGGACGATATTCGTTGGCTTCACGCCATCTTCCGGACTAAGCTAGCAGGTGGTCTGGGAGGGGAGCCTTCCCCGCTATTCTCCGGCCTTAGAACGGGCTTGTGGGAACCCGAGCATGATGAATTTGATGAAGAACACATGGGTTTTGTGCCTTGGGAGCTATGGTGTTTGAGAGAGTTTTCTTTTCAGTTTTGCATGAGATTTCTCCAGCAACTTCAACTAAATTCCATACCCCTT

Protein sequence

MTANPRLSLDHLLLSSIVVSLLNLTENLESVLSVFGAFLDKLVENLVFIYSDNDKQACSNVFMGFSEISIDYMTEILSPRLYGTVCTQVEIELPVPDTLPTSAESSRSSSSSTFQEGQRVSLEEDDIRWLHAIFRTKLAGGLGGEPSPLFSGLRTGLWEPEHDEFDEEHMGFVPWELWCLREFSFQFCMRFLQQLQLNSIPL
Homology
[Close]
BLAST of Spg035470 vs. NCBI nr
Match: TYK27998.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 88.6 bits (218), Expect = 6.8e-14
Identity = 55/103 (53.40%), Postives = 61/103 (59.22%), Query Frame = 0 

Query: 73  MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRSSSSS--- 132
           MTEILSPRLYGTVCTQ                 VEIELPVPDTLPTSAESS S+SS+   
Sbjct: 1   MTEILSPRLYGTVCTQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLE 60

Query: 133 TFQEGQRVSL-------EEDDIRWLHAIFRTKLAGGLGGEPSP 149
            + E   V +        EDD+ WLHA+FR K AGG GG   P
Sbjct: 61  LYVEFVHVEMFWLAGIVREDDVCWLHAVFRAKTAGGPGGGVMP 103

[Close]
BLAST of Spg035470 vs. NCBI nr
Match: KAA0066851.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 88.6 bits (218), Expect = 6.8e-14
Identity = 55/103 (53.40%), Postives = 61/103 (59.22%), Query Frame = 0 

Query: 73  MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRSSSSS--- 132
           MTEILSPRLYGTVCTQ                 VEIELPVPDTLPTSAESS S+SS+   
Sbjct: 1   MTEILSPRLYGTVCTQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLE 60

Query: 133 TFQEGQRVSL-------EEDDIRWLHAIFRTKLAGGLGGEPSP 149
            + E   V +        EDD+ WLHA+FR K AGG GG   P
Sbjct: 61  LYVEFVHVEMFWLAGIVREDDVCWLHAVFRAKTAGGPGGGVMP 103

[Close]
BLAST of Spg035470 vs. NCBI nr
Match: TYK00848.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 87.0 bits (214), Expect = 2.0e-13
Identity = 54/98 (55.10%), Postives = 60/98 (61.22%), Query Frame = 0 

Query: 65  FSEISIDYMTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSR 124
           F+E+SI YMTEILS RLYG VCTQ                 VEIEL VPDTLPTSAES R
Sbjct: 6   FAELSIAYMTEILSLRLYGIVCTQVIFSLSTLSVLHDNDAVVEIELLVPDTLPTSAESFR 65

Query: 125 SSSSSTFQEGQRVSLEEDDIRWLHAIFRTKLAGGLGGE 146
           S+S S         +  DD+ WLHAIFR K AGG GG+
Sbjct: 66  SNSRSA------GIVRGDDVCWLHAIFRAKTAGGPGGD 97

[Close]
BLAST of Spg035470 vs. NCBI nr
Match: TYK11693.1 (gag protease polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 84.3 bits (207), Expect = 1.3e-12
Identity = 50/94 (53.19%), Postives = 58/94 (61.70%), Query Frame = 0 

Query: 65  FSEISIDYMTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSR 124
           F+E+SIDY+TEILS RLYGTVCTQ                 VEIELPVPDTLPTSAESS+
Sbjct: 6   FAELSIDYVTEILSLRLYGTVCTQVIFLLSTLSVLHDNDAVVEIELPVPDTLPTSAESSK 65

Query: 125 SSSSSTFQEGQRVSLEEDDIRWLHAIFRTKLAGG 142
           S+S S         +  DD+ WLH +FR K   G
Sbjct: 66  SNSRSA------GIVRGDDVCWLHVVFRAKTDKG 93

[Close]
BLAST of Spg035470 vs. NCBI nr
Match: TYK01784.1 (hypothetical protein E5676_scaffold113G00120 [Cucumis melo var. makuwa])

HSP 1 Score: 79.7 bits (195), Expect = 3.1e-11
Identity = 49/93 (52.69%), Postives = 54/93 (58.06%), Query Frame = 0 

Query: 68  ISIDYMTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRSSS 127
           +SIDY+TEILS RLYGTVCTQ                 VEIEL VPDTLPTSAESS S S
Sbjct: 655 LSIDYVTEILSLRLYGTVCTQVIFSLSTLSVLRDNDVVVEIELLVPDTLPTSAESSESKS 714

Query: 128 SSTFQEGQRVSLEEDDIRWLHAIFRTKLAGGLG 144
            S         +  DD+ WLH +FR K  GG G
Sbjct: 715 RSV------GIVRGDDVCWLHVVFRAKTVGGPG 741

[Close]
BLAST of Spg035470 vs. ExPASy TrEMBL
Match: A0A5D3DX39 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G002140 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 3.3e-14
Identity = 55/103 (53.40%), Postives = 61/103 (59.22%), Query Frame = 0 

Query: 73  MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRSSSSS--- 132
           MTEILSPRLYGTVCTQ                 VEIELPVPDTLPTSAESS S+SS+   
Sbjct: 1   MTEILSPRLYGTVCTQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLE 60

Query: 133 TFQEGQRVSL-------EEDDIRWLHAIFRTKLAGGLGGEPSP 149
            + E   V +        EDD+ WLHA+FR K AGG GG   P
Sbjct: 61  LYVEFVHVEMFWLAGIVREDDVCWLHAVFRAKTAGGPGGGVMP 103

[Close]
BLAST of Spg035470 vs. ExPASy TrEMBL
Match: A0A5A7VMG9 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold271G002220 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 3.3e-14
Identity = 55/103 (53.40%), Postives = 61/103 (59.22%), Query Frame = 0 

Query: 73  MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRSSSSS--- 132
           MTEILSPRLYGTVCTQ                 VEIELPVPDTLPTSAESS S+SS+   
Sbjct: 1   MTEILSPRLYGTVCTQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLE 60

Query: 133 TFQEGQRVSL-------EEDDIRWLHAIFRTKLAGGLGGEPSP 149
            + E   V +        EDD+ WLHA+FR K AGG GG   P
Sbjct: 61  LYVEFVHVEMFWLAGIVREDDVCWLHAVFRAKTAGGPGGGVMP 103

[Close]
BLAST of Spg035470 vs. ExPASy TrEMBL
Match: A0A5D3BP54 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold509G00090 PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 9.6e-14
Identity = 54/98 (55.10%), Postives = 60/98 (61.22%), Query Frame = 0 

Query: 65  FSEISIDYMTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSR 124
           F+E+SI YMTEILS RLYG VCTQ                 VEIEL VPDTLPTSAES R
Sbjct: 6   FAELSIAYMTEILSLRLYGIVCTQVIFSLSTLSVLHDNDAVVEIELLVPDTLPTSAESFR 65

Query: 125 SSSSSTFQEGQRVSLEEDDIRWLHAIFRTKLAGGLGGE 146
           S+S S         +  DD+ WLHAIFR K AGG GG+
Sbjct: 66  SNSRSA------GIVRGDDVCWLHAIFRAKTAGGPGGD 97

[Close]
BLAST of Spg035470 vs. ExPASy TrEMBL
Match: A0A5D3CIF4 (Gag protease polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold304G00010 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 6.2e-13
Identity = 50/94 (53.19%), Postives = 58/94 (61.70%), Query Frame = 0 

Query: 65  FSEISIDYMTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSR 124
           F+E+SIDY+TEILS RLYGTVCTQ                 VEIELPVPDTLPTSAESS+
Sbjct: 6   FAELSIDYVTEILSLRLYGTVCTQVIFLLSTLSVLHDNDAVVEIELPVPDTLPTSAESSK 65

Query: 125 SSSSSTFQEGQRVSLEEDDIRWLHAIFRTKLAGG 142
           S+S S         +  DD+ WLH +FR K   G
Sbjct: 66  SNSRSA------GIVRGDDVCWLHVVFRAKTDKG 93

[Close]
BLAST of Spg035470 vs. ExPASy TrEMBL
Match: A0A5A7UCL6 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold207G00280 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.5e-11
Identity = 49/93 (52.69%), Postives = 54/93 (58.06%), Query Frame = 0 

Query: 68  ISIDYMTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRSSS 127
           +SIDY+TEILS RLYGTVCTQ                 VEIEL VPDTLPTSAESS S S
Sbjct: 655 LSIDYVTEILSLRLYGTVCTQVIFSLSTLSVLRDNDVVVEIELLVPDTLPTSAESSESKS 714

Query: 128 SSTFQEGQRVSLEEDDIRWLHAIFRTKLAGGLG 144
            S         +  DD+ WLH +FR K  GG G
Sbjct: 715 RSV------GIVRGDDVCWLHVVFRAKTVGGPG 741

The following BLAST results are available for this feature:
BLAST of Spg035470 vs. NCBI nr
Match NameE-valueIdentityDescription
TYK27998.16.8e-1453.40reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0066851.16.8e-1453.40reverse transcriptase [Cucumis melo var. makuwa][more]
TYK00848.12.0e-1355.10gag/pol protein [Cucumis melo var. makuwa][more]
TYK11693.11.3e-1253.19gag protease polyprotein [Cucumis melo var. makuwa][more]
TYK01784.13.1e-1152.69hypothetical protein E5676_scaffold113G00120 [Cucumis melo var. makuwa][more]
BLAST of Spg035470 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Spg035470 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3DX393.3e-1453.40Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38... [more]
A0A5A7VMG93.3e-1453.40Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27... [more]
A0A5D3BP549.6e-1455.10Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold509G0009... [more]
A0A5D3CIF46.2e-1353.19Gag protease polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A5A7UCL61.5e-1152.69Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1... [more]
BLAST of Spg035470 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 99..118
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..119

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg035470.1Spg035470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090304 nucleic acid metabolic process
molecular_function GO:0016740 transferase activity