Spg035430 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg035430
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionpyridoxal reductase, chloroplastic
Locationscaffold3: 1686375 .. 1688776 (-)
RNA-Seq ExpressionSpg035430
SyntenySpg035430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CACCACATAACCCAATCAATCAAATCTCTTCAACAGTCTTTCTCCATCTCCTTCCTTTTACTTGTATCTAGCGTTCTTTCTCTGTAGCTGAAGCTGAAGCCCAAGCTCCAAAACAATGGCAGGAATGGCTTCCTCAACATTTAGCCCCTCCTTCTGCTTCAGATCTTTCGCCGCATCGGAGAAGACTTGTTGTTGTTCTTCTTCTTCTCCGGCGGCTTTCAAGCCACTCAAGCTTCCTCTCTTTTGGCCATGGCAGAAGGTCTCTATTTATTATCCCAATTGAATGGAAATACAATACAATACCCTGCTCTGTGCCTTGAATGGCGTCTCCTTTTGATTGCAGGTGAGGATGGGCCCTCTGAGTGTTTCCCCTATGGGATTCGGAACCTGGGCGTGGGGGAACCAGCTCCTGTGGGGCTACCAGGAATCCATGGACGCTGAGCTTCAACACATTTTCAATTTGGCTGTGAAGAATGGAATCAATCTCTTCGACACTGCGGATTCCTATGGTACTGGAAGACTAAATGGCCAAAGCGAAAAGCTACTTGGAAAATTTATCCGGGAATCTCAAGGTCCATTTTGCTCTTTAATATATTTCTTCATTACTCATCTGTATCCAAGACCTGGTTTCTTTTGCTATTCCTTCTCAAGGAATAATGTTAATTCTCCAGAGAAAAATGAGATTGTGATAGCCACCAAGTTTGCAGCGTATCCATGGCGCCTAACACCTGGCCAGTTTGTCAAAGCTTGCAAGTAATGCTATACATTTTAACCATATTCGTTTGTGAAAGTTTCTACCACACATTTTAGATGACAATTAGCTTTTATTTAGGGCGTCTTTGGATCGAATGCAGATTGAGCAAATTGGTATAGGACAATTACATTGGTCAACAGCAAACTATGCTCCTTTGCAGGAGATGGCCCTTTGGGATGGACTTGTGGCCATGTATGATAAGGTTACTATAATTTGTGATCAAATGCTTCAACTTTCTCAAATTTGCTTCTCTTCATTAAACTAACCCATTAGTTTTCATTTGACGCTATACTCATAAAATTTGTGAATTATAGGGCTTGGTTCAAGCAGTTGGAGTGAGCAATTATGGACCAAAGCAACTGGTAAAGATATATGACTACCTGAAAGACAGAGGAGTCCCCTTATGCTCTGCTCAGGTATTTAGTAAGAACAAGCTATATCAATGTTTTCTATTATGTACTCCATACTTGATAGTGCAACACAAAATGGAATACATGTCCATTTTATGGAATACCTGCTGAGTGCTAACTCTTTCATTTTACTTTTAGGTGCAATTTTCTTTACTAAGCATAGGAGATGACCAGTTGGAGATTAAGAAAATATGTGATTCTTTGGGTATCCGCTTAATTGCTTACAGTCCTTTGGGACTTGGAATGCTCACAGGAAAATATTCACCCAGCAAGCTTCCATCTGGACCTCGGTAAAATAAACAAGAATCCGATGGCATTAATTCTTTCTCGATCTCAAGCAATTTATATTTGCGTTTTCAGGGGTCTGCTCTTCAGGCAAATTCTTCCAGGATTGGAACCTTTGTTGACTACACTTGGCGACATTGCAGAGAAAAGGCGCAAAACGATACCACAGGCAATTCATTTACTTCCCTTTGAGCAATTTAGAAAGCATTGTCTTTCACATTATCTCAGTTTCCAGTGCTTTCCATTTCAAGTTCTTTCTTCATTCTTTTGCCAAATTGACGTGATCCGAGTGGTAACAGGTTGCCATAAACTGGTGCATATCCAAGGGGACCATACCCATACCTGGAGCTAAGACATTGAAACAAGCTCAAGAAAATTTGGGTGCTCTTGGCTGGCGACTTACCCAAGATGAACTGGACCTGTTGGAAGATGGAGCTCGGGAGTCTCCTCGGAGGATGATACAGAATATTTTCCAGACCAGGTGCTTTAAGTTAACTTCATTCTTACATTTTTAAATTCTATTGACCATGTTTTCTTTCAATATTGACCCCTCATTCAAAATCAGGTAAACTTGGCTGCACCAAAAGGCACCCGGGAGAAGCTGCTGAACGTTCATAGCTCACAGGGGGTGTAGTTGAGTTGAAAATGATTCAATTCGGAATATGATGTAACAATAATGAACACTCATTTCATTAATAAAATAGATTCAAGGAGAGAAACCACACAATTACTCATAATTAACGTAAACCAACCAAAATTTGTCAGCAGCCAAACTACAAAAATTGAATACTTAATTAACGAGTGCATAATCTAAGGCTCTACGAGACAGCAAGTGAATCAAGTGACATATCAGGTAACCATATTGCCTTCTCAGGATCAGCAGTCTACCAGCAAGTGAATCAAGTGACATATCAGGTACCATATTGCCTTCTCAGGATCAGCAGTCTACTTGA

mRNA sequence

ATGGCAGGAATGGCTTCCTCAACATTTAGCCCCTCCTTCTGCTTCAGATCTTTCGCCGCATCGGAGAAGACTTGTTGTTGTTCTTCTTCTTCTCCGGCGGCTTTCAAGCCACTCAAGCTTCCTCTCTTTTGGCCATGGCAGAAGGTGAGGATGGGCCCTCTGAGTGTTTCCCCTATGGGATTCGGAACCTGGGCGTGGGGGAACCAGCTCCTGTGGGGCTACCAGGAATCCATGGACGCTGAGCTTCAACACATTTTCAATTTGGCTGTGAAGAATGGAATCAATCTCTTCGACACTGCGGATTCCTATGGTACTGGAAGACTAAATGGCCAAAGCGAAAAGCTACTTGGAAAATTTATCCGGGAATCTCAAGGTCCATTTTGCTCTTTAATATATTTCTTCATTACTCATCTGTATCCAAGACCTGGTTTCTTTTGCTATTCCTTCTCAAGGAATAATGTTAATTCTCCAGAGAAAAATGAGATTGTGATAGCCACCAAGTTTGCAGCGTATCCATGGCGCCTAACACCTGGCCAGTTTGTCAAAGCTTGCAAGGCGTCTTTGGATCGAATGCAGATTGAGCAAATTGGTATAGGACAATTACATTGGTCAACAGCAAACTATGCTCCTTTGCAGGAGATGGCCCTTTGGGATGGACTTGTGGCCATGTATGATAAGGGCTTGGTTCAAGCAGTTGGAGTGAGCAATTATGGACCAAAGCAACTGGTAAAGATATATGACTACCTGAAAGACAGAGGAGTCCCCTTATGCTCTGCTCAGGTGCAATTTTCTTTACTAAGCATAGGAGATGACCAGTTGGAGATTAAGAAAATATGTGATTCTTTGGGTATCCGCTTAATTGCTTACAGTCCTTTGGGACTTGGAATGCTCACAGGAAAATATTCACCCAGCAAGCTTCCATCTGGACCTCGGGGTCTGCTCTTCAGGCAAATTCTTCCAGGATTGGAACCTTTGTTGACTACACTTGGCGACATTGCAGAGAAAAGGCGCAAAACGATACCACAGGCAATTCATTTACTTCCCTTTGAGCAATTTAGAAAGCATTGTCTTTCACATTATCTCAGTTTCCAGTGCTTTCCATTTCAAGTTGCCATAAACTGGTGCATATCCAAGGGGACCATACCCATACCTGGAGCTAAGACATTGAAACAAGCTCAAGAAAATTTGGGTGCTCTTGGCTGGCGACTTACCCAAGATGAACTGGACCTGTTGGAAGATGGAGCTCGGGAGTCTCCTCGGAGGATGATACAGAATATTTTCCAGACCAGGATCAGCAGTCTACCAGCAAGTGAATCAAGTGACATATCAGGTACCATATTGCCTTCTCAGGATCAGCAGTCTACTTGA

Coding sequence (CDS)

ATGGCAGGAATGGCTTCCTCAACATTTAGCCCCTCCTTCTGCTTCAGATCTTTCGCCGCATCGGAGAAGACTTGTTGTTGTTCTTCTTCTTCTCCGGCGGCTTTCAAGCCACTCAAGCTTCCTCTCTTTTGGCCATGGCAGAAGGTGAGGATGGGCCCTCTGAGTGTTTCCCCTATGGGATTCGGAACCTGGGCGTGGGGGAACCAGCTCCTGTGGGGCTACCAGGAATCCATGGACGCTGAGCTTCAACACATTTTCAATTTGGCTGTGAAGAATGGAATCAATCTCTTCGACACTGCGGATTCCTATGGTACTGGAAGACTAAATGGCCAAAGCGAAAAGCTACTTGGAAAATTTATCCGGGAATCTCAAGGTCCATTTTGCTCTTTAATATATTTCTTCATTACTCATCTGTATCCAAGACCTGGTTTCTTTTGCTATTCCTTCTCAAGGAATAATGTTAATTCTCCAGAGAAAAATGAGATTGTGATAGCCACCAAGTTTGCAGCGTATCCATGGCGCCTAACACCTGGCCAGTTTGTCAAAGCTTGCAAGGCGTCTTTGGATCGAATGCAGATTGAGCAAATTGGTATAGGACAATTACATTGGTCAACAGCAAACTATGCTCCTTTGCAGGAGATGGCCCTTTGGGATGGACTTGTGGCCATGTATGATAAGGGCTTGGTTCAAGCAGTTGGAGTGAGCAATTATGGACCAAAGCAACTGGTAAAGATATATGACTACCTGAAAGACAGAGGAGTCCCCTTATGCTCTGCTCAGGTGCAATTTTCTTTACTAAGCATAGGAGATGACCAGTTGGAGATTAAGAAAATATGTGATTCTTTGGGTATCCGCTTAATTGCTTACAGTCCTTTGGGACTTGGAATGCTCACAGGAAAATATTCACCCAGCAAGCTTCCATCTGGACCTCGGGGTCTGCTCTTCAGGCAAATTCTTCCAGGATTGGAACCTTTGTTGACTACACTTGGCGACATTGCAGAGAAAAGGCGCAAAACGATACCACAGGCAATTCATTTACTTCCCTTTGAGCAATTTAGAAAGCATTGTCTTTCACATTATCTCAGTTTCCAGTGCTTTCCATTTCAAGTTGCCATAAACTGGTGCATATCCAAGGGGACCATACCCATACCTGGAGCTAAGACATTGAAACAAGCTCAAGAAAATTTGGGTGCTCTTGGCTGGCGACTTACCCAAGATGAACTGGACCTGTTGGAAGATGGAGCTCGGGAGTCTCCTCGGAGGATGATACAGAATATTTTCCAGACCAGGATCAGCAGTCTACCAGCAAGTGAATCAAGTGACATATCAGGTACCATATTGCCTTCTCAGGATCAGCAGTCTACTTGA

Protein sequence

MAGMASSTFSPSFCFRSFAASEKTCCCSSSSPAAFKPLKLPLFWPWQKVRMGPLSVSPMGFGTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEKLLGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWRLTPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGLGMLTGKYSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFEQFRKHCLSHYLSFQCFPFQVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQDELDLLEDGARESPRRMIQNIFQTRISSLPASESSDISGTILPSQDQQST
Homology
[Close]
BLAST of Spg035430 vs. NCBI nr
Match: XP_022922137.1 (pyridoxal reductase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 672.5 bits (1734), Expect = 2.5e-189
Identity = 345/432 (79.86%), Postives = 359/432 (83.10%), Query Frame = 0 

Query: 1   MAGMASSTFSPSFCFRSF--AASEKTCCCSSSSPAAFKPLKLPLFWPWQKVRMGPLSVSP 60
           MAGMASSTF+PSFCFRSF  AAS K    SSSSPAAFKPLKLPLFWPWQKV+MGPLS+SP
Sbjct: 1   MAGMASSTFTPSFCFRSFAVAASNKASSSSSSSPAAFKPLKLPLFWPWQKVKMGPLSLSP 60

Query: 61  MGFGTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEKLLGK 120
           MGFGTWAWGNQLLWGYQESMDAELQ IFNLAV+NGINLFDTADSYGTGRLNGQSEKLLGK
Sbjct: 61  MGFGTWAWGNQLLWGYQESMDAELQQIFNLAVENGINLFDTADSYGTGRLNGQSEKLLGK 120

Query: 121 FIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWRLTPG 180
           FIRESQ                                 EKN+IVIATKFAAYPWRLTPG
Sbjct: 121 FIRESQ---------------------------------EKNDIVIATKFAAYPWRLTPG 180

Query: 181 QFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYG 240
           QFVKACKASLDRMQIE+IGIGQLHWSTANYAPLQEMALWDGLVAMYD+GLVQAVGVSNYG
Sbjct: 181 QFVKACKASLDRMQIEKIGIGQLHWSTANYAPLQEMALWDGLVAMYDQGLVQAVGVSNYG 240

Query: 241 PKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGLGMLT 300
           PKQLVKI+DYLKDRGVPLCSAQVQFSLLSIGD+QLE KKICDSLGIRLIAYSPLGLGMLT
Sbjct: 241 PKQLVKIHDYLKDRGVPLCSAQVQFSLLSIGDEQLETKKICDSLGIRLIAYSPLGLGMLT 300

Query: 301 GKYSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFEQFRKHCLS 360
           GKYSPSKLPSGPRGLLFRQILPGLEPLL+TLG+IAEKRRKTIP                 
Sbjct: 301 GKYSPSKLPSGPRGLLFRQILPGLEPLLSTLGNIAEKRRKTIP----------------- 360

Query: 361 HYLSFQCFPFQVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQDELDLLEDGARES 420
                     QVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQ+E +LLEDGAR S
Sbjct: 361 ----------QVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQEESNLLEDGARAS 372

Query: 421 PRRMIQNIFQTR 431
             RMIQN+FQTR
Sbjct: 421 TGRMIQNVFQTR 372

[Close]
BLAST of Spg035430 vs. NCBI nr
Match: KAG6579301.1 (Pyridoxal reductase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 668.3 bits (1723), Expect = 4.7e-188
Identity = 343/436 (78.67%), Postives = 358/436 (82.11%), Query Frame = 0 

Query: 1   MAGMASSTFSPSFCFRSFA------ASEKTCCCSSSSPAAFKPLKLPLFWPWQKVRMGPL 60
           MAGMASSTF+PSFCFRSF       AS  +   SSSSPAAFKPLKLPLFWPWQKV+MGPL
Sbjct: 1   MAGMASSTFTPSFCFRSFTVAASNKASSSSSSSSSSSPAAFKPLKLPLFWPWQKVKMGPL 60

Query: 61  SVSPMGFGTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEK 120
           SVSPMGFGTWAWGNQLLWGYQESMDAELQ IFNLAV+NGINLFDTADSYGTGRLNGQSEK
Sbjct: 61  SVSPMGFGTWAWGNQLLWGYQESMDAELQQIFNLAVENGINLFDTADSYGTGRLNGQSEK 120

Query: 121 LLGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWR 180
           LLGKFIRESQ                                 EKN+IVIATKFAAYPWR
Sbjct: 121 LLGKFIRESQ---------------------------------EKNDIVIATKFAAYPWR 180

Query: 181 LTPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGV 240
           LTPGQFVKACKASLDRMQIE+IGIGQLHWSTANYAPLQEMALWDGLVAMYD+GLVQAVGV
Sbjct: 181 LTPGQFVKACKASLDRMQIEKIGIGQLHWSTANYAPLQEMALWDGLVAMYDQGLVQAVGV 240

Query: 241 SNYGPKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGL 300
           SNYGPKQLVKI+DYLKDRGVPLCSAQVQFSLLS+GD+QLE KKICDSLGIRLIAYSPLGL
Sbjct: 241 SNYGPKQLVKIHDYLKDRGVPLCSAQVQFSLLSLGDEQLETKKICDSLGIRLIAYSPLGL 300

Query: 301 GMLTGKYSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFEQFRK 360
           GMLTGKYSPSKLPSGPRGLLFRQILPGLEPLL+TLG+IAEKRRKTIP             
Sbjct: 301 GMLTGKYSPSKLPSGPRGLLFRQILPGLEPLLSTLGNIAEKRRKTIP------------- 360

Query: 361 HCLSHYLSFQCFPFQVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQDELDLLEDG 420
                         QVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQ+E +LLEDG
Sbjct: 361 --------------QVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQEESNLLEDG 376

Query: 421 ARESPRRMIQNIFQTR 431
           AR S  RMIQN+FQTR
Sbjct: 421 ARASTGRMIQNVFQTR 376

[Close]
BLAST of Spg035430 vs. NCBI nr
Match: XP_023549646.1 (pyridoxal reductase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 667.5 bits (1721), Expect = 8.0e-188
Identity = 346/435 (79.54%), Postives = 359/435 (82.53%), Query Frame = 0 

Query: 1   MAGMASSTFSPSFCFRSF--AASEK---TCCCSSSSPAAFKPLKLPLFWPWQKVRMGPLS 60
           MAGMASSTFSPSFCFRSF  AAS K   +   SSSSPAAFKPLKLPLFWPWQKV+MGPLS
Sbjct: 1   MAGMASSTFSPSFCFRSFAVAASNKASSSSSSSSSSPAAFKPLKLPLFWPWQKVKMGPLS 60

Query: 61  VSPMGFGTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEKL 120
           VSPMGFGTWAWGNQLLWGYQESMDAELQ IFNLAV+NGINLFDTADSYGTGRLNGQSEKL
Sbjct: 61  VSPMGFGTWAWGNQLLWGYQESMDAELQQIFNLAVENGINLFDTADSYGTGRLNGQSEKL 120

Query: 121 LGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWRL 180
           LGKFIRESQ                                 EKN+IVIATKFAAYPWRL
Sbjct: 121 LGKFIRESQ---------------------------------EKNDIVIATKFAAYPWRL 180

Query: 181 TPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVS 240
           TP QFVKACKASLDRMQIE+IGIGQLHWSTANYAPLQEMALWDGLVAMYD+GLVQAVGVS
Sbjct: 181 TPAQFVKACKASLDRMQIEKIGIGQLHWSTANYAPLQEMALWDGLVAMYDQGLVQAVGVS 240

Query: 241 NYGPKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGLG 300
           NYGPKQLVKI+DYLKDRGVPLCSAQVQFSLLSIGD+QLE KKICDSLGIRLIAYSPLGLG
Sbjct: 241 NYGPKQLVKIHDYLKDRGVPLCSAQVQFSLLSIGDEQLETKKICDSLGIRLIAYSPLGLG 300

Query: 301 MLTGKYSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFEQFRKH 360
           MLTGKYSPSKLPSGPRGLLFRQILPGLEPLL+TLG+IAEKRRKTIP              
Sbjct: 301 MLTGKYSPSKLPSGPRGLLFRQILPGLEPLLSTLGNIAEKRRKTIP-------------- 360

Query: 361 CLSHYLSFQCFPFQVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQDELDLLEDGA 420
                        QVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQ+E +LLEDGA
Sbjct: 361 -------------QVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQEESNLLEDGA 375

Query: 421 RESPRRMIQNIFQTR 431
           R S  RMIQN+FQTR
Sbjct: 421 RASTGRMIQNVFQTR 375

[Close]
BLAST of Spg035430 vs. NCBI nr
Match: XP_022974350.1 (pyridoxal reductase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 665.6 bits (1716), Expect = 3.0e-187
Identity = 345/436 (79.13%), Postives = 357/436 (81.88%), Query Frame = 0 

Query: 1   MAGMASSTFSPSFCFRSF--AASEKT----CCCSSSSPAAFKPLKLPLFWPWQKVRMGPL 60
           MAGMASSTFSPSFCFRSF  AAS KT       SSSS AAFKPLKLPLFWPWQKV+MGPL
Sbjct: 41  MAGMASSTFSPSFCFRSFATAASNKTSSSSSSSSSSSQAAFKPLKLPLFWPWQKVKMGPL 100

Query: 61  SVSPMGFGTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEK 120
           SVSPMGFGTWAWGNQLLWGYQESMDAELQ IFNLAV+NGINLFDTADSYGTGRLNGQSEK
Sbjct: 101 SVSPMGFGTWAWGNQLLWGYQESMDAELQQIFNLAVENGINLFDTADSYGTGRLNGQSEK 160

Query: 121 LLGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWR 180
           LLGKFIRESQ                                 EKN+IV+ATKFAAYPWR
Sbjct: 161 LLGKFIRESQ---------------------------------EKNDIVVATKFAAYPWR 220

Query: 181 LTPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGV 240
           LTPGQFVKACKASLDRMQIE+IGIGQLHWSTANYAPLQEMALWDGLVAMYD+GLVQAVGV
Sbjct: 221 LTPGQFVKACKASLDRMQIEKIGIGQLHWSTANYAPLQEMALWDGLVAMYDQGLVQAVGV 280

Query: 241 SNYGPKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGL 300
           SNYGPKQLVKI+DYLKDRGVPLCSAQVQFSLLSIGD+QLE KKICDSLGIRLIAYSPLGL
Sbjct: 281 SNYGPKQLVKIHDYLKDRGVPLCSAQVQFSLLSIGDEQLETKKICDSLGIRLIAYSPLGL 340

Query: 301 GMLTGKYSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFEQFRK 360
           GMLTGKYSPSKLPSGPRGLLFRQILPGLEPLL+TLG IAEKRRKTIP             
Sbjct: 341 GMLTGKYSPSKLPSGPRGLLFRQILPGLEPLLSTLGSIAEKRRKTIP------------- 400

Query: 361 HCLSHYLSFQCFPFQVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQDELDLLEDG 420
                         QVAINWCISKGTIPIPG KTLKQAQENLGALGWRLTQ+E +LLEDG
Sbjct: 401 --------------QVAINWCISKGTIPIPGVKTLKQAQENLGALGWRLTQEESNLLEDG 416

Query: 421 ARESPRRMIQNIFQTR 431
           AR S  RMIQN+FQTR
Sbjct: 461 ARASTGRMIQNVFQTR 416

[Close]
BLAST of Spg035430 vs. NCBI nr
Match: XP_008437647.1 (PREDICTED: pyridoxal reductase, chloroplastic [Cucumis melo])

HSP 1 Score: 656.8 bits (1693), Expect = 1.4e-184
Identity = 335/430 (77.91%), Postives = 352/430 (81.86%), Query Frame = 0 

Query: 1   MAGMASSTFSPSFCFRSFAASEKTCCCSSSSPAAFKPLKLPLFWPWQKVRMGPLSVSPMG 60
           MA MAS   SPS CF SF A+      SSSSPAAFKPLKLPLFWPWQKV+MGPLSVSPMG
Sbjct: 26  MAQMASFALSPS-CFGSFTAAASNKASSSSSPAAFKPLKLPLFWPWQKVKMGPLSVSPMG 85

Query: 61  FGTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEKLLGKFI 120
           FGTWAWGNQLLWGYQESMD+ELQHIFNLAV NGINLFDTADSYGTGRLNGQSEKLLGKFI
Sbjct: 86  FGTWAWGNQLLWGYQESMDSELQHIFNLAVDNGINLFDTADSYGTGRLNGQSEKLLGKFI 145

Query: 121 RESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWRLTPGQF 180
           R+SQ                                  KNEIVIATKFAAYPWRLTP QF
Sbjct: 146 RQSQ---------------------------------VKNEIVIATKFAAYPWRLTPAQF 205

Query: 181 VKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPK 240
           VKAC+ SL+RMQ+E+IGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPK
Sbjct: 206 VKACQGSLERMQMEKIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPK 265

Query: 241 QLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGLGMLTGK 300
           QLV+IYDYLKDRGVPLC+AQVQFSLLSIGDDQLE+KKICDSLGIRLIAYSPLGLGMLTGK
Sbjct: 266 QLVRIYDYLKDRGVPLCTAQVQFSLLSIGDDQLEVKKICDSLGIRLIAYSPLGLGMLTGK 325

Query: 301 YSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFEQFRKHCLSHY 360
           YSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIP                   
Sbjct: 326 YSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIP------------------- 385

Query: 361 LSFQCFPFQVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQDELDLLEDGARESPR 420
                   QVAINWCISKGTIPIPGAKTLKQAQENLGALGW LTQ+EL+LL++GA+ESPR
Sbjct: 386 --------QVAINWCISKGTIPIPGAKTLKQAQENLGALGWNLTQEELNLLDNGAQESPR 394

Query: 421 RMIQNIFQTR 431
           +MIQNIFQTR
Sbjct: 446 KMIQNIFQTR 394

[Close]
BLAST of Spg035430 vs. ExPASy Swiss-Prot
Match: Q56Y42 (Pyridoxal reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLR1 PE=1 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 9.9e-157
Identity = 273/406 (67.24%), Postives = 312/406 (76.85%), Query Frame = 0 

Query: 25  CCCSSSSPAAFKPLKLPLFWPWQKVRMGPLSVSPMGFGTWAWGNQLLWGYQESMDAELQH 84
           C  ++S+   FKPLKLPLFWPWQKV+MGPLSVSPMGFGTWAWGNQLLWGYQ SMD +LQ 
Sbjct: 16  CSNNTSNITTFKPLKLPLFWPWQKVKMGPLSVSPMGFGTWAWGNQLLWGYQTSMDDQLQQ 75

Query: 85  IFNLAVKNGINLFDTADSYGTGRLNGQSEKLLGKFIRESQGPFCSLIYFFITHLYPRPGF 144
            F LA++NGINLFDTADSYGTGRLNGQSE+LLGKFI+ESQG                   
Sbjct: 76  AFELALENGINLFDTADSYGTGRLNGQSERLLGKFIKESQG------------------- 135

Query: 145 FCYSFSRNNVNSPEKNEIVIATKFAAYPWRLTPGQFVKACKASLDRMQIEQIGIGQLHWS 204
                        ++NE+V+ATKFAAYPWRLT GQFV AC+ASLDR+QI+Q+GIGQLHWS
Sbjct: 136 ----------LKGKQNEVVVATKFAAYPWRLTSGQFVNACRASLDRLQIDQLGIGQLHWS 195

Query: 205 TANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPKQLVKIYDYLKDRGVPLCSAQVQFS 264
           TA+YAPLQE+ LWDGLV MY+KGLV+AVGVSNYGP+QLVKI+DYLK RGVPLCSAQVQFS
Sbjct: 196 TASYAPLQELVLWDGLVQMYEKGLVRAVGVSNYGPQQLVKIHDYLKTRGVPLCSAQVQFS 255

Query: 265 LLSIGDDQLEIKKICDSLGIRLIAYSPLGLGMLTGKYSPSKLPSGPRGLLFRQILPGLEP 324
           LLS+G +QLEIK ICD LGIRLI+YSPLGLGMLTGKYS SKLP+GPR LLFRQILPGLEP
Sbjct: 256 LLSMGKEQLEIKSICDELGIRLISYSPLGLGMLTGKYSSSKLPTGPRSLLFRQILPGLEP 315

Query: 325 LLTTLGDIAEKRRKTIPQAIHLLPFEQFRKHCLSHYLSFQCFPFQVAINWCISKGTIPIP 384
           LL  L +IA+KR KT+P                           QVAINWCI KGT+PIP
Sbjct: 316 LLLALSEIAKKRGKTMP---------------------------QVAINWCICKGTVPIP 365

Query: 385 GAKTLKQAQENLGALGWRLTQDELDLLEDGARESPRRMIQNIFQTR 431
           G K+++  ++NLGALGW+LT DE   LE  A+ESP+ MIQNIFQTR
Sbjct: 376 GIKSVRHVEDNLGALGWKLTNDEQLQLEYAAKESPKSMIQNIFQTR 365

[Close]
BLAST of Spg035430 vs. ExPASy Swiss-Prot
Match: Q94A68 (Uncharacterized oxidoreductase At1g06690, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g06690 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 5.0e-60
Identity = 145/385 (37.66%), Postives = 198/385 (51.43%), Query Frame = 0 

Query: 48  KVRMG--PLSVSPMGFGTWAWGNQLLWGYQESMDAEL---QHIFNLAVKNGINLFDTADS 107
           KV++G   L V+ +G G W+WG+   W   +  D +L   +  F+ ++ NGI+ FDTA+ 
Sbjct: 48  KVKLGGSDLKVTKLGIGVWSWGDNSYWNDFQWDDRKLKAAKGAFDTSLDNGIDFFDTAEV 107

Query: 108 YGTGRLNG--QSEKLLGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKN 167
           YG+    G   SE LLG+FIRE +  +              PG                 
Sbjct: 108 YGSKFSLGAISSETLLGRFIRERKERY--------------PG----------------A 167

Query: 168 EIVIATKFAAYPWRLTPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALW--- 227
           E+ +ATKFAA PWR      V A K SL R+++  + + QLHW            LW   
Sbjct: 168 EVSVATKFAALPWRFGRESVVTALKDSLSRLELSSVDLYQLHWP----------GLWGNE 227

Query: 228 ---DGLVAMYDKGLVQAVGVSNYGPKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLE 287
              DGL    ++GLV+AVGVSNY  K+L   Y+ LK RG+PL S QV +SL+    +Q  
Sbjct: 228 GYLDGLGDAVEQGLVKAVGVSNYSEKRLRDAYERLKKRGIPLASNQVNYSLIYRAPEQTG 287

Query: 288 IKKICDSLGIRLIAYSPLGLGMLTGKYSPSKLPSGPRGLLF-RQILPGLEPLLTTLGDIA 347
           +K  CD LG+ LIAYSP+  G LTGKY+P   PSGPRG ++ R+ L  L+PLL  +  I 
Sbjct: 288 VKAACDELGVTLIAYSPIAQGALTGKYTPENPPSGPRGRIYTREFLTKLQPLLNRIKQIG 347

Query: 348 EKRRKTIPQAIHLLPFEQFRKHCLSHYLSFQCFPFQVAINWCISKG-TIPIPGAKTLKQA 407
           E   KT                           P Q+A+NW +++G  IPIPGAK  +QA
Sbjct: 348 ENYSKT---------------------------PTQIALNWLVAQGNVIPIPGAKNAEQA 365

Query: 408 QENLGALGWRLTQDELDLLEDGARE 418
           +E  GA+GW LT +E+  L   A E
Sbjct: 408 KEFAGAIGWSLTDNEVSELRSLASE 365

[Close]
BLAST of Spg035430 vs. ExPASy Swiss-Prot
Match: P9WQA6 (Uncharacterized oxidoreductase MT2355 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2355 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.3e-23
Identity = 112/387 (28.94%), Postives = 165/387 (42.64%), Query Frame = 0 

Query: 56  VSPMGFGTWAWGNQLLWGYQES-MDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEK 115
           VS +G GTW +G++  WGY +       + I   A   G+ LFDTA+ YG G+    SE+
Sbjct: 12  VSRIGLGTWQFGSR-EWGYGDRYATGAARDIVKRARALGVTLFDTAEIYGLGK----SER 71

Query: 116 LLGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWR 175
           +LG+ + +                                   ++ E+V+A+K   +P  
Sbjct: 72  ILGEALGD-----------------------------------DRTEVVVASK--VFPVA 131

Query: 176 LTPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGV 235
             P       +AS  R+Q+ +I + Q+H        + +  +  G+  + D G + A GV
Sbjct: 132 PFPAVIKNRERASARRLQLNRIPLYQIHQPN---PVVPDSVIMPGMRDLLDSGDIGAAGV 191

Query: 236 SNYGPKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRL-IAYSPLG 295
           SNY   +  K    L   G P+ S QV FSL     D LE       L  R+ IAYSPL 
Sbjct: 192 SNYSLARWRKADAAL---GRPVVSNQVHFSLAH--PDALEDLVPFAELENRIVIAYSPLA 251

Query: 296 LGMLTGKYSPSKLPSGPRGL--LF-RQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFE 355
            G+L GKY     P G R L  LF  + L  +EPLL TL  IA                 
Sbjct: 252 QGLLGGKYGLENRPGGVRALNPLFGTENLRRIEPLLATLRAIA----------------- 311

Query: 356 QFRKHCLSHYLSFQCFPFQVAINWCIS-KGTIPIPGAKTLKQAQENLGALGWRLTQDELD 415
                     +     P QVA+ W IS  G + IPGA +++Q + N+ A    L+    D
Sbjct: 312 ----------VDVDAKPAQVALAWLISLPGVVAIPGASSVEQLEFNVAAADIELSAQSRD 321

Query: 416 LLEDGARE----SPRRMIQNIFQTRIS 433
            L D AR     S  R + ++ + ++S
Sbjct: 372 ALTDAARAFRPVSTGRFLTDMVREKVS 321

[Close]
BLAST of Spg035430 vs. ExPASy Swiss-Prot
Match: P9WQA7 (Uncharacterized oxidoreductase Rv2298 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2298 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.3e-23
Identity = 112/387 (28.94%), Postives = 165/387 (42.64%), Query Frame = 0 

Query: 56  VSPMGFGTWAWGNQLLWGYQES-MDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEK 115
           VS +G GTW +G++  WGY +       + I   A   G+ LFDTA+ YG G+    SE+
Sbjct: 12  VSRIGLGTWQFGSR-EWGYGDRYATGAARDIVKRARALGVTLFDTAEIYGLGK----SER 71

Query: 116 LLGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWR 175
           +LG+ + +                                   ++ E+V+A+K   +P  
Sbjct: 72  ILGEALGD-----------------------------------DRTEVVVASK--VFPVA 131

Query: 176 LTPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGV 235
             P       +AS  R+Q+ +I + Q+H        + +  +  G+  + D G + A GV
Sbjct: 132 PFPAVIKNRERASARRLQLNRIPLYQIHQPN---PVVPDSVIMPGMRDLLDSGDIGAAGV 191

Query: 236 SNYGPKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRL-IAYSPLG 295
           SNY   +  K    L   G P+ S QV FSL     D LE       L  R+ IAYSPL 
Sbjct: 192 SNYSLARWRKADAAL---GRPVVSNQVHFSLAH--PDALEDLVPFAELENRIVIAYSPLA 251

Query: 296 LGMLTGKYSPSKLPSGPRGL--LF-RQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFE 355
            G+L GKY     P G R L  LF  + L  +EPLL TL  IA                 
Sbjct: 252 QGLLGGKYGLENRPGGVRALNPLFGTENLRRIEPLLATLRAIA----------------- 311

Query: 356 QFRKHCLSHYLSFQCFPFQVAINWCIS-KGTIPIPGAKTLKQAQENLGALGWRLTQDELD 415
                     +     P QVA+ W IS  G + IPGA +++Q + N+ A    L+    D
Sbjct: 312 ----------VDVDAKPAQVALAWLISLPGVVAIPGASSVEQLEFNVAAADIELSAQSRD 321

Query: 416 LLEDGARE----SPRRMIQNIFQTRIS 433
            L D AR     S  R + ++ + ++S
Sbjct: 372 ALTDAARAFRPVSTGRFLTDMVREKVS 321

[Close]
BLAST of Spg035430 vs. ExPASy Swiss-Prot
Match: P63485 (Uncharacterized oxidoreductase Mb2320 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2320 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.3e-23
Identity = 112/387 (28.94%), Postives = 165/387 (42.64%), Query Frame = 0 

Query: 56  VSPMGFGTWAWGNQLLWGYQES-MDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEK 115
           VS +G GTW +G++  WGY +       + I   A   G+ LFDTA+ YG G+    SE+
Sbjct: 12  VSRIGLGTWQFGSR-EWGYGDRYATGAARDIVKRARALGVTLFDTAEIYGLGK----SER 71

Query: 116 LLGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWR 175
           +LG+ + +                                   ++ E+V+A+K   +P  
Sbjct: 72  ILGEALGD-----------------------------------DRTEVVVASK--VFPVA 131

Query: 176 LTPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGV 235
             P       +AS  R+Q+ +I + Q+H        + +  +  G+  + D G + A GV
Sbjct: 132 PFPAVIKNRERASARRLQLNRIPLYQIHQPN---PVVPDSVIMPGMRDLLDSGDIGAAGV 191

Query: 236 SNYGPKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRL-IAYSPLG 295
           SNY   +  K    L   G P+ S QV FSL     D LE       L  R+ IAYSPL 
Sbjct: 192 SNYSLARWRKADAAL---GRPVVSNQVHFSLAH--PDALEDLVPFAELENRIVIAYSPLA 251

Query: 296 LGMLTGKYSPSKLPSGPRGL--LF-RQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFE 355
            G+L GKY     P G R L  LF  + L  +EPLL TL  IA                 
Sbjct: 252 QGLLGGKYGLENRPGGVRALNPLFGTENLRRIEPLLATLRAIA----------------- 311

Query: 356 QFRKHCLSHYLSFQCFPFQVAINWCIS-KGTIPIPGAKTLKQAQENLGALGWRLTQDELD 415
                     +     P QVA+ W IS  G + IPGA +++Q + N+ A    L+    D
Sbjct: 312 ----------VDVDAKPAQVALAWLISLPGVVAIPGASSVEQLEFNVAAADIELSAQSRD 321

Query: 416 LLEDGARE----SPRRMIQNIFQTRIS 433
            L D AR     S  R + ++ + ++S
Sbjct: 372 ALTDAARAFRPVSTGRFLTDMVREKVS 321

[Close]
BLAST of Spg035430 vs. ExPASy TrEMBL
Match: A0A6J1E2D6 (pyridoxal reductase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430205 PE=4 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 1.2e-189
Identity = 345/432 (79.86%), Postives = 359/432 (83.10%), Query Frame = 0 

Query: 1   MAGMASSTFSPSFCFRSF--AASEKTCCCSSSSPAAFKPLKLPLFWPWQKVRMGPLSVSP 60
           MAGMASSTF+PSFCFRSF  AAS K    SSSSPAAFKPLKLPLFWPWQKV+MGPLS+SP
Sbjct: 1   MAGMASSTFTPSFCFRSFAVAASNKASSSSSSSPAAFKPLKLPLFWPWQKVKMGPLSLSP 60

Query: 61  MGFGTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEKLLGK 120
           MGFGTWAWGNQLLWGYQESMDAELQ IFNLAV+NGINLFDTADSYGTGRLNGQSEKLLGK
Sbjct: 61  MGFGTWAWGNQLLWGYQESMDAELQQIFNLAVENGINLFDTADSYGTGRLNGQSEKLLGK 120

Query: 121 FIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWRLTPG 180
           FIRESQ                                 EKN+IVIATKFAAYPWRLTPG
Sbjct: 121 FIRESQ---------------------------------EKNDIVIATKFAAYPWRLTPG 180

Query: 181 QFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYG 240
           QFVKACKASLDRMQIE+IGIGQLHWSTANYAPLQEMALWDGLVAMYD+GLVQAVGVSNYG
Sbjct: 181 QFVKACKASLDRMQIEKIGIGQLHWSTANYAPLQEMALWDGLVAMYDQGLVQAVGVSNYG 240

Query: 241 PKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGLGMLT 300
           PKQLVKI+DYLKDRGVPLCSAQVQFSLLSIGD+QLE KKICDSLGIRLIAYSPLGLGMLT
Sbjct: 241 PKQLVKIHDYLKDRGVPLCSAQVQFSLLSIGDEQLETKKICDSLGIRLIAYSPLGLGMLT 300

Query: 301 GKYSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFEQFRKHCLS 360
           GKYSPSKLPSGPRGLLFRQILPGLEPLL+TLG+IAEKRRKTIP                 
Sbjct: 301 GKYSPSKLPSGPRGLLFRQILPGLEPLLSTLGNIAEKRRKTIP----------------- 360

Query: 361 HYLSFQCFPFQVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQDELDLLEDGARES 420
                     QVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQ+E +LLEDGAR S
Sbjct: 361 ----------QVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQEESNLLEDGARAS 372

Query: 421 PRRMIQNIFQTR 431
             RMIQN+FQTR
Sbjct: 421 TGRMIQNVFQTR 372

[Close]
BLAST of Spg035430 vs. ExPASy TrEMBL
Match: A0A6J1IDQ8 (pyridoxal reductase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472975 PE=4 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.5e-187
Identity = 345/436 (79.13%), Postives = 357/436 (81.88%), Query Frame = 0 

Query: 1   MAGMASSTFSPSFCFRSF--AASEKT----CCCSSSSPAAFKPLKLPLFWPWQKVRMGPL 60
           MAGMASSTFSPSFCFRSF  AAS KT       SSSS AAFKPLKLPLFWPWQKV+MGPL
Sbjct: 41  MAGMASSTFSPSFCFRSFATAASNKTSSSSSSSSSSSQAAFKPLKLPLFWPWQKVKMGPL 100

Query: 61  SVSPMGFGTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEK 120
           SVSPMGFGTWAWGNQLLWGYQESMDAELQ IFNLAV+NGINLFDTADSYGTGRLNGQSEK
Sbjct: 101 SVSPMGFGTWAWGNQLLWGYQESMDAELQQIFNLAVENGINLFDTADSYGTGRLNGQSEK 160

Query: 121 LLGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWR 180
           LLGKFIRESQ                                 EKN+IV+ATKFAAYPWR
Sbjct: 161 LLGKFIRESQ---------------------------------EKNDIVVATKFAAYPWR 220

Query: 181 LTPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGV 240
           LTPGQFVKACKASLDRMQIE+IGIGQLHWSTANYAPLQEMALWDGLVAMYD+GLVQAVGV
Sbjct: 221 LTPGQFVKACKASLDRMQIEKIGIGQLHWSTANYAPLQEMALWDGLVAMYDQGLVQAVGV 280

Query: 241 SNYGPKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGL 300
           SNYGPKQLVKI+DYLKDRGVPLCSAQVQFSLLSIGD+QLE KKICDSLGIRLIAYSPLGL
Sbjct: 281 SNYGPKQLVKIHDYLKDRGVPLCSAQVQFSLLSIGDEQLETKKICDSLGIRLIAYSPLGL 340

Query: 301 GMLTGKYSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFEQFRK 360
           GMLTGKYSPSKLPSGPRGLLFRQILPGLEPLL+TLG IAEKRRKTIP             
Sbjct: 341 GMLTGKYSPSKLPSGPRGLLFRQILPGLEPLLSTLGSIAEKRRKTIP------------- 400

Query: 361 HCLSHYLSFQCFPFQVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQDELDLLEDG 420
                         QVAINWCISKGTIPIPG KTLKQAQENLGALGWRLTQ+E +LLEDG
Sbjct: 401 --------------QVAINWCISKGTIPIPGVKTLKQAQENLGALGWRLTQEESNLLEDG 416

Query: 421 ARESPRRMIQNIFQTR 431
           AR S  RMIQN+FQTR
Sbjct: 461 ARASTGRMIQNVFQTR 416

[Close]
BLAST of Spg035430 vs. ExPASy TrEMBL
Match: A0A5D3C6C2 (Pyridoxal reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00210 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 6.8e-185
Identity = 335/430 (77.91%), Postives = 352/430 (81.86%), Query Frame = 0 

Query: 1   MAGMASSTFSPSFCFRSFAASEKTCCCSSSSPAAFKPLKLPLFWPWQKVRMGPLSVSPMG 60
           MA MAS   SPS CF SF A+      SSSSPAAFKPLKLPLFWPWQKV+MGPLSVSPMG
Sbjct: 1   MAQMASFALSPS-CFGSFTAAASNKASSSSSPAAFKPLKLPLFWPWQKVKMGPLSVSPMG 60

Query: 61  FGTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEKLLGKFI 120
           FGTWAWGNQLLWGYQESMD+ELQHIFNLAV NGINLFDTADSYGTGRLNGQSEKLLGKFI
Sbjct: 61  FGTWAWGNQLLWGYQESMDSELQHIFNLAVDNGINLFDTADSYGTGRLNGQSEKLLGKFI 120

Query: 121 RESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWRLTPGQF 180
           R+SQ                                  KNEIVIATKFAAYPWRLTP QF
Sbjct: 121 RQSQ---------------------------------VKNEIVIATKFAAYPWRLTPAQF 180

Query: 181 VKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPK 240
           VKAC+ SL+RMQ+E+IGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPK
Sbjct: 181 VKACQGSLERMQMEKIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPK 240

Query: 241 QLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGLGMLTGK 300
           QLV+IYDYLKDRGVPLC+AQVQFSLLSIGDDQLE+KKICDSLGIRLIAYSPLGLGMLTGK
Sbjct: 241 QLVRIYDYLKDRGVPLCTAQVQFSLLSIGDDQLEVKKICDSLGIRLIAYSPLGLGMLTGK 300

Query: 301 YSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFEQFRKHCLSHY 360
           YSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIP                   
Sbjct: 301 YSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIP------------------- 360

Query: 361 LSFQCFPFQVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQDELDLLEDGARESPR 420
                   QVAINWCISKGTIPIPGAKTLKQAQENLGALGW LTQ+EL+LL++GA+ESPR
Sbjct: 361 --------QVAINWCISKGTIPIPGAKTLKQAQENLGALGWNLTQEELNLLDNGAQESPR 369

Query: 421 RMIQNIFQTR 431
           +MIQNIFQTR
Sbjct: 421 KMIQNIFQTR 369

[Close]
BLAST of Spg035430 vs. ExPASy TrEMBL
Match: A0A1S3AU73 (pyridoxal reductase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103482991 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 6.8e-185
Identity = 335/430 (77.91%), Postives = 352/430 (81.86%), Query Frame = 0 

Query: 1   MAGMASSTFSPSFCFRSFAASEKTCCCSSSSPAAFKPLKLPLFWPWQKVRMGPLSVSPMG 60
           MA MAS   SPS CF SF A+      SSSSPAAFKPLKLPLFWPWQKV+MGPLSVSPMG
Sbjct: 26  MAQMASFALSPS-CFGSFTAAASNKASSSSSPAAFKPLKLPLFWPWQKVKMGPLSVSPMG 85

Query: 61  FGTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEKLLGKFI 120
           FGTWAWGNQLLWGYQESMD+ELQHIFNLAV NGINLFDTADSYGTGRLNGQSEKLLGKFI
Sbjct: 86  FGTWAWGNQLLWGYQESMDSELQHIFNLAVDNGINLFDTADSYGTGRLNGQSEKLLGKFI 145

Query: 121 RESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWRLTPGQF 180
           R+SQ                                  KNEIVIATKFAAYPWRLTP QF
Sbjct: 146 RQSQ---------------------------------VKNEIVIATKFAAYPWRLTPAQF 205

Query: 181 VKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPK 240
           VKAC+ SL+RMQ+E+IGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPK
Sbjct: 206 VKACQGSLERMQMEKIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPK 265

Query: 241 QLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGLGMLTGK 300
           QLV+IYDYLKDRGVPLC+AQVQFSLLSIGDDQLE+KKICDSLGIRLIAYSPLGLGMLTGK
Sbjct: 266 QLVRIYDYLKDRGVPLCTAQVQFSLLSIGDDQLEVKKICDSLGIRLIAYSPLGLGMLTGK 325

Query: 301 YSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFEQFRKHCLSHY 360
           YSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIP                   
Sbjct: 326 YSPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIP------------------- 385

Query: 361 LSFQCFPFQVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQDELDLLEDGARESPR 420
                   QVAINWCISKGTIPIPGAKTLKQAQENLGALGW LTQ+EL+LL++GA+ESPR
Sbjct: 386 --------QVAINWCISKGTIPIPGAKTLKQAQENLGALGWNLTQEELNLLDNGAQESPR 394

Query: 421 RMIQNIFQTR 431
           +MIQNIFQTR
Sbjct: 446 KMIQNIFQTR 394

[Close]
BLAST of Spg035430 vs. ExPASy TrEMBL
Match: A0A0A0KJR5 (Aldo_ket_red domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139120 PE=4 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 4.4e-184
Identity = 338/429 (78.79%), Postives = 353/429 (82.28%), Query Frame = 0 

Query: 4   MASSTFSPSFCFRSF--AASEKTCCCSSSSPAAFKPLKLPLFWPWQKVRMGPLSVSPMGF 63
           MASST +PS CF SF  AAS KT   S SSPAAFKPLKLPLFWPWQKV+MGPLSVSPMGF
Sbjct: 1   MASSTLTPS-CFGSFAAAASNKT---SYSSPAAFKPLKLPLFWPWQKVKMGPLSVSPMGF 60

Query: 64  GTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSEKLLGKFIR 123
           GTWAWGNQLLWGYQESMD+ELQHIFNLAV NGINLFDTADSYGTGRLNGQSEKLLGKFIR
Sbjct: 61  GTWAWGNQLLWGYQESMDSELQHIFNLAVDNGINLFDTADSYGTGRLNGQSEKLLGKFIR 120

Query: 124 ESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPWRLTPGQFV 183
           +SQ                                  KN+IVIATKFAAYPWRLTP QFV
Sbjct: 121 QSQ---------------------------------VKNDIVIATKFAAYPWRLTPAQFV 180

Query: 184 KACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPKQ 243
           KAC+ SL+RMQIE+IGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPKQ
Sbjct: 181 KACQGSLERMQIEKIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPKQ 240

Query: 244 LVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGLGMLTGKY 303
           LV+I+DYLKDRGVPLC+AQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGLGMLTGKY
Sbjct: 241 LVRIHDYLKDRGVPLCTAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLGLGMLTGKY 300

Query: 304 SPSKLPSGPRGLLFRQILPGLEPLLTTLGDIAEKRRKTIPQAIHLLPFEQFRKHCLSHYL 363
           SPSKLPSGPRGLLFRQ LPGLEPLLTTLGDIAEKRRKTIP                    
Sbjct: 301 SPSKLPSGPRGLLFRQTLPGLEPLLTTLGDIAEKRRKTIP-------------------- 360

Query: 364 SFQCFPFQVAINWCISKGTIPIPGAKTLKQAQENLGALGWRLTQDELDLLEDGARESPRR 423
                  QVAINWCISKGTIPIPGAKTLKQAQENLGALGW LTQ+EL+LL+DGARESPR+
Sbjct: 361 -------QVAINWCISKGTIPIPGAKTLKQAQENLGALGWNLTQEELNLLDDGARESPRK 365

Query: 424 MIQNIFQTR 431
           MIQNIFQTR
Sbjct: 421 MIQNIFQTR 365

[Close]
BLAST of Spg035430 vs. TAIR 10
Match: AT5G53580.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 554.7 bits (1428), Expect = 7.0e-158
Identity = 273/406 (67.24%), Postives = 312/406 (76.85%), Query Frame = 0 

Query: 25  CCCSSSSPAAFKPLKLPLFWPWQKVRMGPLSVSPMGFGTWAWGNQLLWGYQESMDAELQH 84
           C  ++S+   FKPLKLPLFWPWQKV+MGPLSVSPMGFGTWAWGNQLLWGYQ SMD +LQ 
Sbjct: 16  CSNNTSNITTFKPLKLPLFWPWQKVKMGPLSVSPMGFGTWAWGNQLLWGYQTSMDDQLQQ 75

Query: 85  IFNLAVKNGINLFDTADSYGTGRLNGQSEKLLGKFIRESQGPFCSLIYFFITHLYPRPGF 144
            F LA++NGINLFDTADSYGTGRLNGQSE+LLGKFI+ESQG                   
Sbjct: 76  AFELALENGINLFDTADSYGTGRLNGQSERLLGKFIKESQG------------------- 135

Query: 145 FCYSFSRNNVNSPEKNEIVIATKFAAYPWRLTPGQFVKACKASLDRMQIEQIGIGQLHWS 204
                        ++NE+V+ATKFAAYPWRLT GQFV AC+ASLDR+QI+Q+GIGQLHWS
Sbjct: 136 ----------LKGKQNEVVVATKFAAYPWRLTSGQFVNACRASLDRLQIDQLGIGQLHWS 195

Query: 205 TANYAPLQEMALWDGLVAMYDKGLVQAVGVSNYGPKQLVKIYDYLKDRGVPLCSAQVQFS 264
           TA+YAPLQE+ LWDGLV MY+KGLV+AVGVSNYGP+QLVKI+DYLK RGVPLCSAQVQFS
Sbjct: 196 TASYAPLQELVLWDGLVQMYEKGLVRAVGVSNYGPQQLVKIHDYLKTRGVPLCSAQVQFS 255

Query: 265 LLSIGDDQLEIKKICDSLGIRLIAYSPLGLGMLTGKYSPSKLPSGPRGLLFRQILPGLEP 324
           LLS+G +QLEIK ICD LGIRLI+YSPLGLGMLTGKYS SKLP+GPR LLFRQILPGLEP
Sbjct: 256 LLSMGKEQLEIKSICDELGIRLISYSPLGLGMLTGKYSSSKLPTGPRSLLFRQILPGLEP 315

Query: 325 LLTTLGDIAEKRRKTIPQAIHLLPFEQFRKHCLSHYLSFQCFPFQVAINWCISKGTIPIP 384
           LL  L +IA+KR KT+P                           QVAINWCI KGT+PIP
Sbjct: 316 LLLALSEIAKKRGKTMP---------------------------QVAINWCICKGTVPIP 365

Query: 385 GAKTLKQAQENLGALGWRLTQDELDLLEDGARESPRRMIQNIFQTR 431
           G K+++  ++NLGALGW+LT DE   LE  A+ESP+ MIQNIFQTR
Sbjct: 376 GIKSVRHVEDNLGALGWKLTNDEQLQLEYAAKESPKSMIQNIFQTR 365

[Close]
BLAST of Spg035430 vs. TAIR 10
Match: AT1G06690.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 3.6e-61
Identity = 145/385 (37.66%), Postives = 198/385 (51.43%), Query Frame = 0 

Query: 48  KVRMG--PLSVSPMGFGTWAWGNQLLWGYQESMDAEL---QHIFNLAVKNGINLFDTADS 107
           KV++G   L V+ +G G W+WG+   W   +  D +L   +  F+ ++ NGI+ FDTA+ 
Sbjct: 48  KVKLGGSDLKVTKLGIGVWSWGDNSYWNDFQWDDRKLKAAKGAFDTSLDNGIDFFDTAEV 107

Query: 108 YGTGRLNG--QSEKLLGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKN 167
           YG+    G   SE LLG+FIRE +  +              PG                 
Sbjct: 108 YGSKFSLGAISSETLLGRFIRERKERY--------------PG----------------A 167

Query: 168 EIVIATKFAAYPWRLTPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALW--- 227
           E+ +ATKFAA PWR      V A K SL R+++  + + QLHW            LW   
Sbjct: 168 EVSVATKFAALPWRFGRESVVTALKDSLSRLELSSVDLYQLHWP----------GLWGNE 227

Query: 228 ---DGLVAMYDKGLVQAVGVSNYGPKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLE 287
              DGL    ++GLV+AVGVSNY  K+L   Y+ LK RG+PL S QV +SL+    +Q  
Sbjct: 228 GYLDGLGDAVEQGLVKAVGVSNYSEKRLRDAYERLKKRGIPLASNQVNYSLIYRAPEQTG 287

Query: 288 IKKICDSLGIRLIAYSPLGLGMLTGKYSPSKLPSGPRGLLF-RQILPGLEPLLTTLGDIA 347
           +K  CD LG+ LIAYSP+  G LTGKY+P   PSGPRG ++ R+ L  L+PLL  +  I 
Sbjct: 288 VKAACDELGVTLIAYSPIAQGALTGKYTPENPPSGPRGRIYTREFLTKLQPLLNRIKQIG 347

Query: 348 EKRRKTIPQAIHLLPFEQFRKHCLSHYLSFQCFPFQVAINWCISKG-TIPIPGAKTLKQA 407
           E   KT                           P Q+A+NW +++G  IPIPGAK  +QA
Sbjct: 348 ENYSKT---------------------------PTQIALNWLVAQGNVIPIPGAKNAEQA 365

Query: 408 QENLGALGWRLTQDELDLLEDGARE 418
           +E  GA+GW LT +E+  L   A E
Sbjct: 408 KEFAGAIGWSLTDNEVSELRSLASE 365

[Close]
BLAST of Spg035430 vs. TAIR 10
Match: AT1G04690.1 (potassium channel beta subunit 1 )

HSP 1 Score: 80.5 bits (197), Expect = 3.9e-15
Identity = 97/388 (25.00%), Postives = 158/388 (40.72%), Query Frame = 0 

Query: 54  LSVSPMGFGTW-AWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQS 113
           L VS + FG W  +GNQL          E + I      +G+N FD A+ Y     NG++
Sbjct: 11  LKVSTLSFGAWVTFGNQL-------DVKEAKSILQCCRDHGVNFFDNAEVYA----NGRA 70

Query: 114 EKLLGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATK-FAAY 173
           E+++G+ IRE                                    +++IVI+TK F   
Sbjct: 71  EEIMGQAIREL--------------------------------GWRRSDIVISTKIFWGG 130

Query: 174 PW----RLTPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKG 233
           P      L+    V+  KASL R+ ++ + +   H   A+  P++E      +  + DKG
Sbjct: 131 PGPNDKGLSRKHIVEGTKASLKRLDMDYVDVLYCHRPDAS-TPIEETV--RAMNYVIDKG 190

Query: 234 LVQAVGVSNYGPKQLVKIY---DYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGI 293
                G S +  +Q+ + +   D L D   P+   Q ++++ +    + E   +  + GI
Sbjct: 191 WAFYWGTSEWSAQQITEAWGAADRL-DLVGPIVE-QPEYNMFARHKVETEFLPLYTNHGI 250

Query: 294 RLIAYSPLGLGMLTGKYSPSKLPSGPR-------GLLFRQILPGLEPLLTTLGDIAEKRR 353
            L  +SPL  G+LTGKY+   +PS  R        L  R ++  +   ++ L  IA++  
Sbjct: 251 GLTTWSPLASGVLTGKYNKGAIPSDSRFALENYKNLANRSLVDDVLRKVSGLKPIADELG 310

Query: 354 KTIPQAIHLLPFEQFRKHCLSHYLSFQCFPFQVAINWCISKGTIP--IPGAKTLKQAQEN 413
            T+                            Q+AI WC S   +   I GA    Q QEN
Sbjct: 311 VTLA---------------------------QLAIAWCASNPNVSSVITGATRESQIQEN 323

Query: 414 LGALGW--RLTQDELDLLEDGARESPRR 422
           + A+     LT   LD +E   +  P+R
Sbjct: 371 MKAVDVIPLLTPIVLDKIEQVIQSKPKR 323

[Close]
BLAST of Spg035430 vs. TAIR 10
Match: AT1G60680.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 2.5e-14
Identity = 94/373 (25.20%), Postives = 151/373 (40.48%), Query Frame = 0 

Query: 54  LSVSPMGFGTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYGTGRLNGQSE 113
           L VS  G G  A   +      E+    L H    A+ +G+  FDT+D YG       +E
Sbjct: 17  LEVSAQGLGCMALSARYGAPKPETDAIALLH---HAINSGVTFFDTSDMYGP----ETNE 76

Query: 114 KLLGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVIATKFAAYPW 173
            LLGK +++       L   F        GFF        +   E +E+           
Sbjct: 77  LLLGKALKDGVKEKVELATKF--------GFF--------IVEGEISEV----------- 136

Query: 174 RLTPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWDGLVAMYDKGLVQAVG 233
           R  P     AC+ASL R+ I  I +   H       P+ E+ + + L  + ++G ++ +G
Sbjct: 137 RGDPEYVRAACEASLKRLDIACIDLYYQH-RIDTRVPI-EITMRE-LKKLVEEGKIKYIG 196

Query: 234 VSNYGPKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKICDSLGIRLIAYSPLG 293
           +S      + + +        P+ + Q+++SL S  D + +I  IC  LGI ++AYSPLG
Sbjct: 197 LSEASASTIRRAHAV-----HPITAVQIEWSLWS-RDAEEDIIPICRELGIGIVAYSPLG 256

Query: 294 LGMLTGKYSPSKLPSGPRGLLFRQILPGLEP--------LLTTLGDIAEKRRKTIPQAIH 353
            G L    +  KL        FR+ LP  +         L   +  +AEK+         
Sbjct: 257 RGFLA---AGPKLAENLENDDFRKTLPRFQQENVDHNKILFEKVSAMAEKK--------- 316

Query: 354 LLPFEQFRKHCLSHYLSFQCFPFQVAINWCISKG--TIPIPGAKTLKQAQENLGALGWRL 413
                              C P Q+A+ W   +G    PIPG   ++   +N+ AL  +L
Sbjct: 317 ------------------GCTPAQLALAWVHHQGDDVCPIPGTTKIENLNQNIRALSVKL 316

Query: 414 TQDELDLLEDGAR 417
           T +E+  L+  A+
Sbjct: 377 TPEEISELDSLAK 316

[Close]
BLAST of Spg035430 vs. TAIR 10
Match: AT1G10810.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 77.0 bits (188), Expect = 4.3e-14
Identity = 88/384 (22.92%), Postives = 157/384 (40.89%), Query Frame = 0 

Query: 47  QKVRMGP--LSVSPMGFGTWAWGNQLLWGYQESMDAELQHIFNLAVKNGINLFDTADSYG 106
           +++++G   L VS  G G        ++     ++ +L  + + A+ +GI L DT+D YG
Sbjct: 8   RRIKLGSQGLEVSAQGLGCMGLS---IFDGTTKVETDLIALIHHAINSGITLLDTSDIYG 67

Query: 107 TGRLNGQSEKLLGKFIRESQGPFCSLIYFFITHLYPRPGFFCYSFSRNNVNSPEKNEIVI 166
                  +E LLG+ +++                                    + ++ +
Sbjct: 68  P----ETNELLLGQALKDGM----------------------------------REKVEL 127

Query: 167 ATKFA------AYPWRLTPGQFVKACKASLDRMQIEQIGIGQLHWSTANYAPLQEMALWD 226
           ATKF          +R  P     AC+ASL R+ +  I +   H       P+ E+ + +
Sbjct: 128 ATKFGLLLKDQKLGYRGDPAYVRAACEASLRRLGVSCIDLYYQH-RIDTTVPI-EVTIGE 187

Query: 227 GLVAMYDKGLVQAVGVSNYGPKQLVKIYDYLKDRGVPLCSAQVQFSLLSIGDDQLEIKKI 286
            L  + ++G ++ +G+S      + + +        PL + Q+++SL S  D + +I   
Sbjct: 188 -LKKLVEEGKIKYIGLSEACASTIRRAHAV-----HPLTAVQLEWSLWS-RDVEEDIIPT 247

Query: 287 CDSLGIRLIAYSPLGLGMLTGKYSPSKLPSGPRGLLFRQILPGLEP--------LLTTLG 346
           C  LGI ++AYSPLGLG       P  + S   G  +R+ LP  +         L   + 
Sbjct: 248 CRELGIGIVAYSPLGLGFFAA--GPKFIESMDNG-DYRKGLPRFQQENLDHNKILYEKVN 307

Query: 347 DIAEKRRKTIPQAIHLLPFEQFRKHCLSHYLSFQCFPFQVAINWCISKG--TIPIPGAKT 406
            +AEK+                            C P Q+A+ W   +G    PIPG   
Sbjct: 308 AMAEKK---------------------------SCTPAQLALAWVHHQGNDVCPIPGTSK 311

Query: 407 LKQAQENLGALGWRLTQDELDLLE 413
           +K   +N+GAL  +L+ +E+  L+
Sbjct: 368 IKNLNQNIGALSVKLSIEEMAELD 311

The following BLAST results are available for this feature:
BLAST of Spg035430 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022922137.12.5e-18979.86pyridoxal reductase, chloroplastic [Cucurbita moschata][more]
KAG6579301.14.7e-18878.67Pyridoxal reductase, chloroplastic, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023549646.18.0e-18879.54pyridoxal reductase, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022974350.13.0e-18779.13pyridoxal reductase, chloroplastic [Cucurbita maxima][more]
XP_008437647.11.4e-18477.91PREDICTED: pyridoxal reductase, chloroplastic [Cucumis melo][more]
BLAST of Spg035430 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q56Y429.9e-15767.24Pyridoxal reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PLR1 PE=1 ... [more]
Q94A685.0e-6037.66Uncharacterized oxidoreductase At1g06690, chloroplastic OS=Arabidopsis thaliana ... [more]
P9WQA61.3e-2328.94Uncharacterized oxidoreductase MT2355 OS=Mycobacterium tuberculosis (strain CDC ... [more]
P9WQA71.3e-2328.94Uncharacterized oxidoreductase Rv2298 OS=Mycobacterium tuberculosis (strain ATCC... [more]
P634851.3e-2328.94Uncharacterized oxidoreductase Mb2320 OS=Mycobacterium bovis (strain ATCC BAA-93... [more]
BLAST of Spg035430 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1E2D61.2e-18979.86pyridoxal reductase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430205... [more]
A0A6J1IDQ81.5e-18779.13pyridoxal reductase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472975 P... [more]
A0A5D3C6C26.8e-18577.91Pyridoxal reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G... [more]
A0A1S3AU736.8e-18577.91pyridoxal reductase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103482991 PE=4 ... [more]
A0A0A0KJR54.4e-18478.79Aldo_ket_red domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G13912... [more]
BLAST of Spg035430 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G53580.17.0e-15867.24NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G06690.13.6e-6137.66NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G04690.13.9e-1525.00potassium channel beta subunit 1 [more]
AT1G60680.12.5e-1425.20NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G10810.14.3e-1422.92NAD(P)-linked oxidoreductase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020471Aldo-keto reductasePRINTSPR00069ALDKETRDTASEcoord: 220..237
score: 41.44
coord: 89..113
score: 30.87
IPR036812NADP-dependent oxidoreductase domain superfamilyGENE3D3.20.20.100coord: 50..433
e-value: 3.6E-67
score: 228.6
IPR036812NADP-dependent oxidoreductase domain superfamilySUPERFAMILY51430NAD(P)-linked oxidoreductasecoord: 54..418
IPR023210NADP-dependent oxidoreductase domainPFAMPF00248Aldo_ket_redcoord: 58..343
e-value: 1.2E-43
score: 149.4
coord: 367..412
e-value: 9.7E-7
score: 28.2
NoneNo IPR availablePIRSRPIRSR000097-1PIRSR000097-1coord: 59..112
e-value: 0.0028
score: 14.6
NoneNo IPR availablePANTHERPTHR43625AFLATOXIN B1 ALDEHYDE REDUCTASEcoord: 157..342
NoneNo IPR availablePANTHERPTHR43625AFLATOXIN B1 ALDEHYDE REDUCTASEcoord: 37..125
coord: 369..423
NoneNo IPR availablePANTHERPTHR43625:SF5PYRIDOXAL REDUCTASE, CHLOROPLASTICcoord: 37..125
coord: 369..423
coord: 157..342
NoneNo IPR availableCDDcd19093AKR_AtPLR-likecoord: 56..412
e-value: 2.03262E-149
score: 425.103
IPR018170Aldo/keto reductase, conserved sitePROSITEPS00062ALDOKETO_REDUCTASE_2coord: 220..237

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg035430.1Spg035430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004033 aldo-keto reductase (NADP) activity
molecular_function GO:0047834 D-threo-aldose 1-dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity