Spg035427 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg035427 vs. NCBI nr
Match: XP_023551404.1 (E3 ubiquitin-protein ligase SHPRH isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 877.9 bits (2267), Expect = 5.8e-251
Identity = 524/844 (62.09%), Postives = 555/844 (65.76%), Query Frame = 0

Query: 1    MDDSENYDNHITKKQKVSETLYASCSDTEKIQDNPLEPTRKDTNTKKEENYEPHWSSSFL 60
            MDDSE  DNHI KKQKV+ TLY  CSDTEKI D+PLE T +D N KKEENYEPH SSS+ 
Sbjct: 879  MDDSEKSDNHIIKKQKVNGTLYVPCSDTEKIIDSPLELTGQDENAKKEENYEPHRSSSYF 938

Query: 61   NDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLVE 120
            ND+ LRK CEAMRQKYLAVFSSKLSIAQQEFTKSYVQVC                    E
Sbjct: 939  NDNYLRKACEAMRQKYLAVFSSKLSIAQQEFTKSYVQVC-------------------NE 998

Query: 121  LKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSGN-NSKSSRVGSR---INALKY 180
            LK RENLNE+WWL+AVHHAE+NKDFSHELIRKIEE VSGN N+  SRVGSR   I+ALKY
Sbjct: 999  LKSRENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSIDALKY 1058

Query: 181  HVQTGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNVPPCVLCELDE 240
            HVQTGLDLLEASRK+VL RLLEIDQTMENPK+EDIERVRYCQNCQADSN PPCVLCELDE
Sbjct: 1059 HVQTGLDLLEASRKLVLGRLLEIDQTMENPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1118

Query: 241  LFQ--------------------------------------------------------- 300
            LFQ                                                         
Sbjct: 1119 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1178

Query: 301  ----------VSKNPSELEVVLGVIKNFCKAQLGKETIAAANKQLHLLEYMRKEYGHARS 360
                      VSK+PSELEVVLGVIKNFCK QLGKE+IAAANKQLHLLEYMRKEYGHARS
Sbjct: 1179 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1238

Query: 361  LAIAQAQVLNAHDEIKMATTRLCLKDNDDDSSADAISEHELPAASVQYSSDKFMSLAMLS 420
            LAIAQAQVLNAHDEIKMATTRLCL+ NDDD SA AISEHELPAASVQYSSDKF+SLAMLS
Sbjct: 1239 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSDKFVSLAMLS 1298

Query: 421  RVKGKLRYLKVKKFPDSFCALKFFIFRTVMSIPCLGLPSVVIIFPLDCVAILHPTYNFYY 480
            RVKGKLRYLK                                                  
Sbjct: 1299 RVKGKLRYLK-------------------------------------------------- 1358

Query: 481  FQGLVQSKQNIPVGSSSNLTLTQEPAITSTSMEQESENTLKADEESCPV----------- 540
              GLVQSKQNIP+GSSSN   TQ P ITSTS+EQ+SENTLKADEESCPV           
Sbjct: 1359 --GLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQEALSNQKMV 1418

Query: 541  ------------FPVLE------------------------------------------- 600
                        F + E                                           
Sbjct: 1419 FQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTL 1478

Query: 601  -----EHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 660
                 E E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN
Sbjct: 1479 HKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAAN 1538

Query: 661  SITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLN 676
            +ITF+RMKGGRKSHTAI EFRGQKIN KE+HKKQ   +VPELRSAQVLLLLIQHGANGLN
Sbjct: 1539 NITFVRMKGGRKSHTAISEFRGQKINTKENHKKQPLAEVPELRSAQVLLLLIQHGANGLN 1598

BLAST of Spg035427 vs. NCBI nr
Match: XP_023551410.1 (E3 ubiquitin-protein ligase SHPRH isoform X8 [Cucurbita pepo subsp. pepo] >XP_023551411.1 E3 ubiquitin-protein ligase SHPRH isoform X8 [Cucurbita pepo subsp. pepo] >XP_023551412.1 E3 ubiquitin-protein ligase SHPRH isoform X8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 877.9 bits (2267), Expect = 5.8e-251
Identity = 524/844 (62.09%), Postives = 555/844 (65.76%), Query Frame = 0

Query: 1    MDDSENYDNHITKKQKVSETLYASCSDTEKIQDNPLEPTRKDTNTKKEENYEPHWSSSFL 60
            MDDSE  DNHI KKQKV+ TLY  CSDTEKI D+PLE T +D N KKEENYEPH SSS+ 
Sbjct: 614  MDDSEKSDNHIIKKQKVNGTLYVPCSDTEKIIDSPLELTGQDENAKKEENYEPHRSSSYF 673

Query: 61   NDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLVE 120
            ND+ LRK CEAMRQKYLAVFSSKLSIAQQEFTKSYVQVC                    E
Sbjct: 674  NDNYLRKACEAMRQKYLAVFSSKLSIAQQEFTKSYVQVC-------------------NE 733

Query: 121  LKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSGN-NSKSSRVGSR---INALKY 180
            LK RENLNE+WWL+AVHHAE+NKDFSHELIRKIEE VSGN N+  SRVGSR   I+ALKY
Sbjct: 734  LKSRENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSIDALKY 793

Query: 181  HVQTGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNVPPCVLCELDE 240
            HVQTGLDLLEASRK+VL RLLEIDQTMENPK+EDIERVRYCQNCQADSN PPCVLCELDE
Sbjct: 794  HVQTGLDLLEASRKLVLGRLLEIDQTMENPKDEDIERVRYCQNCQADSNGPPCVLCELDE 853

Query: 241  LFQ--------------------------------------------------------- 300
            LFQ                                                         
Sbjct: 854  LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 913

Query: 301  ----------VSKNPSELEVVLGVIKNFCKAQLGKETIAAANKQLHLLEYMRKEYGHARS 360
                      VSK+PSELEVVLGVIKNFCK QLGKE+IAAANKQLHLLEYMRKEYGHARS
Sbjct: 914  NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 973

Query: 361  LAIAQAQVLNAHDEIKMATTRLCLKDNDDDSSADAISEHELPAASVQYSSDKFMSLAMLS 420
            LAIAQAQVLNAHDEIKMATTRLCL+ NDDD SA AISEHELPAASVQYSSDKF+SLAMLS
Sbjct: 974  LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSDKFVSLAMLS 1033

Query: 421  RVKGKLRYLKVKKFPDSFCALKFFIFRTVMSIPCLGLPSVVIIFPLDCVAILHPTYNFYY 480
            RVKGKLRYLK                                                  
Sbjct: 1034 RVKGKLRYLK-------------------------------------------------- 1093

Query: 481  FQGLVQSKQNIPVGSSSNLTLTQEPAITSTSMEQESENTLKADEESCPV----------- 540
              GLVQSKQNIP+GSSSN   TQ P ITSTS+EQ+SENTLKADEESCPV           
Sbjct: 1094 --GLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQEALSNQKMV 1153

Query: 541  ------------FPVLE------------------------------------------- 600
                        F + E                                           
Sbjct: 1154 FQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTL 1213

Query: 601  -----EHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 660
                 E E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN
Sbjct: 1214 HKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAAN 1273

Query: 661  SITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLN 676
            +ITF+RMKGGRKSHTAI EFRGQKIN KE+HKKQ   +VPELRSAQVLLLLIQHGANGLN
Sbjct: 1274 NITFVRMKGGRKSHTAISEFRGQKINTKENHKKQPLAEVPELRSAQVLLLLIQHGANGLN 1333

BLAST of Spg035427 vs. NCBI nr
Match: XP_023551406.1 (E3 ubiquitin-protein ligase SHPRH isoform X5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 877.9 bits (2267), Expect = 5.8e-251
Identity = 524/844 (62.09%), Postives = 555/844 (65.76%), Query Frame = 0

Query: 1    MDDSENYDNHITKKQKVSETLYASCSDTEKIQDNPLEPTRKDTNTKKEENYEPHWSSSFL 60
            MDDSE  DNHI KKQKV+ TLY  CSDTEKI D+PLE T +D N KKEENYEPH SSS+ 
Sbjct: 855  MDDSEKSDNHIIKKQKVNGTLYVPCSDTEKIIDSPLELTGQDENAKKEENYEPHRSSSYF 914

Query: 61   NDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLVE 120
            ND+ LRK CEAMRQKYLAVFSSKLSIAQQEFTKSYVQVC                    E
Sbjct: 915  NDNYLRKACEAMRQKYLAVFSSKLSIAQQEFTKSYVQVC-------------------NE 974

Query: 121  LKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSGN-NSKSSRVGSR---INALKY 180
            LK RENLNE+WWL+AVHHAE+NKDFSHELIRKIEE VSGN N+  SRVGSR   I+ALKY
Sbjct: 975  LKSRENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSIDALKY 1034

Query: 181  HVQTGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNVPPCVLCELDE 240
            HVQTGLDLLEASRK+VL RLLEIDQTMENPK+EDIERVRYCQNCQADSN PPCVLCELDE
Sbjct: 1035 HVQTGLDLLEASRKLVLGRLLEIDQTMENPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1094

Query: 241  LFQ--------------------------------------------------------- 300
            LFQ                                                         
Sbjct: 1095 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1154

Query: 301  ----------VSKNPSELEVVLGVIKNFCKAQLGKETIAAANKQLHLLEYMRKEYGHARS 360
                      VSK+PSELEVVLGVIKNFCK QLGKE+IAAANKQLHLLEYMRKEYGHARS
Sbjct: 1155 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1214

Query: 361  LAIAQAQVLNAHDEIKMATTRLCLKDNDDDSSADAISEHELPAASVQYSSDKFMSLAMLS 420
            LAIAQAQVLNAHDEIKMATTRLCL+ NDDD SA AISEHELPAASVQYSSDKF+SLAMLS
Sbjct: 1215 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSDKFVSLAMLS 1274

Query: 421  RVKGKLRYLKVKKFPDSFCALKFFIFRTVMSIPCLGLPSVVIIFPLDCVAILHPTYNFYY 480
            RVKGKLRYLK                                                  
Sbjct: 1275 RVKGKLRYLK-------------------------------------------------- 1334

Query: 481  FQGLVQSKQNIPVGSSSNLTLTQEPAITSTSMEQESENTLKADEESCPV----------- 540
              GLVQSKQNIP+GSSSN   TQ P ITSTS+EQ+SENTLKADEESCPV           
Sbjct: 1335 --GLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQEALSNQKMV 1394

Query: 541  ------------FPVLE------------------------------------------- 600
                        F + E                                           
Sbjct: 1395 FQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTL 1454

Query: 601  -----EHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 660
                 E E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN
Sbjct: 1455 HKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAAN 1514

Query: 661  SITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLN 676
            +ITF+RMKGGRKSHTAI EFRGQKIN KE+HKKQ   +VPELRSAQVLLLLIQHGANGLN
Sbjct: 1515 NITFVRMKGGRKSHTAISEFRGQKINTKENHKKQPLAEVPELRSAQVLLLLIQHGANGLN 1574

BLAST of Spg035427 vs. NCBI nr
Match: XP_023551408.1 (E3 ubiquitin-protein ligase SHPRH isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 877.9 bits (2267), Expect = 5.8e-251
Identity = 524/844 (62.09%), Postives = 555/844 (65.76%), Query Frame = 0

Query: 1    MDDSENYDNHITKKQKVSETLYASCSDTEKIQDNPLEPTRKDTNTKKEENYEPHWSSSFL 60
            MDDSE  DNHI KKQKV+ TLY  CSDTEKI D+PLE T +D N KKEENYEPH SSS+ 
Sbjct: 626  MDDSEKSDNHIIKKQKVNGTLYVPCSDTEKIIDSPLELTGQDENAKKEENYEPHRSSSYF 685

Query: 61   NDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLVE 120
            ND+ LRK CEAMRQKYLAVFSSKLSIAQQEFTKSYVQVC                    E
Sbjct: 686  NDNYLRKACEAMRQKYLAVFSSKLSIAQQEFTKSYVQVC-------------------NE 745

Query: 121  LKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSGN-NSKSSRVGSR---INALKY 180
            LK RENLNE+WWL+AVHHAE+NKDFSHELIRKIEE VSGN N+  SRVGSR   I+ALKY
Sbjct: 746  LKSRENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSIDALKY 805

Query: 181  HVQTGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNVPPCVLCELDE 240
            HVQTGLDLLEASRK+VL RLLEIDQTMENPK+EDIERVRYCQNCQADSN PPCVLCELDE
Sbjct: 806  HVQTGLDLLEASRKLVLGRLLEIDQTMENPKDEDIERVRYCQNCQADSNGPPCVLCELDE 865

Query: 241  LFQ--------------------------------------------------------- 300
            LFQ                                                         
Sbjct: 866  LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 925

Query: 301  ----------VSKNPSELEVVLGVIKNFCKAQLGKETIAAANKQLHLLEYMRKEYGHARS 360
                      VSK+PSELEVVLGVIKNFCK QLGKE+IAAANKQLHLLEYMRKEYGHARS
Sbjct: 926  NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 985

Query: 361  LAIAQAQVLNAHDEIKMATTRLCLKDNDDDSSADAISEHELPAASVQYSSDKFMSLAMLS 420
            LAIAQAQVLNAHDEIKMATTRLCL+ NDDD SA AISEHELPAASVQYSSDKF+SLAMLS
Sbjct: 986  LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSDKFVSLAMLS 1045

Query: 421  RVKGKLRYLKVKKFPDSFCALKFFIFRTVMSIPCLGLPSVVIIFPLDCVAILHPTYNFYY 480
            RVKGKLRYLK                                                  
Sbjct: 1046 RVKGKLRYLK-------------------------------------------------- 1105

Query: 481  FQGLVQSKQNIPVGSSSNLTLTQEPAITSTSMEQESENTLKADEESCPV----------- 540
              GLVQSKQNIP+GSSSN   TQ P ITSTS+EQ+SENTLKADEESCPV           
Sbjct: 1106 --GLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQEALSNQKMV 1165

Query: 541  ------------FPVLE------------------------------------------- 600
                        F + E                                           
Sbjct: 1166 FQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTL 1225

Query: 601  -----EHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 660
                 E E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN
Sbjct: 1226 HKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAAN 1285

Query: 661  SITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLN 676
            +ITF+RMKGGRKSHTAI EFRGQKIN KE+HKKQ   +VPELRSAQVLLLLIQHGANGLN
Sbjct: 1286 NITFVRMKGGRKSHTAISEFRGQKINTKENHKKQPLAEVPELRSAQVLLLLIQHGANGLN 1345

BLAST of Spg035427 vs. NCBI nr
Match: XP_023551409.1 (E3 ubiquitin-protein ligase SHPRH isoform X7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 877.9 bits (2267), Expect = 5.8e-251
Identity = 524/844 (62.09%), Postives = 555/844 (65.76%), Query Frame = 0

Query: 1    MDDSENYDNHITKKQKVSETLYASCSDTEKIQDNPLEPTRKDTNTKKEENYEPHWSSSFL 60
            MDDSE  DNHI KKQKV+ TLY  CSDTEKI D+PLE T +D N KKEENYEPH SSS+ 
Sbjct: 624  MDDSEKSDNHIIKKQKVNGTLYVPCSDTEKIIDSPLELTGQDENAKKEENYEPHRSSSYF 683

Query: 61   NDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLVE 120
            ND+ LRK CEAMRQKYLAVFSSKLSIAQQEFTKSYVQVC                    E
Sbjct: 684  NDNYLRKACEAMRQKYLAVFSSKLSIAQQEFTKSYVQVC-------------------NE 743

Query: 121  LKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSGN-NSKSSRVGSR---INALKY 180
            LK RENLNE+WWL+AVHHAE+NKDFSHELIRKIEE VSGN N+  SRVGSR   I+ALKY
Sbjct: 744  LKSRENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSIDALKY 803

Query: 181  HVQTGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNVPPCVLCELDE 240
            HVQTGLDLLEASRK+VL RLLEIDQTMENPK+EDIERVRYCQNCQADSN PPCVLCELDE
Sbjct: 804  HVQTGLDLLEASRKLVLGRLLEIDQTMENPKDEDIERVRYCQNCQADSNGPPCVLCELDE 863

Query: 241  LFQ--------------------------------------------------------- 300
            LFQ                                                         
Sbjct: 864  LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 923

Query: 301  ----------VSKNPSELEVVLGVIKNFCKAQLGKETIAAANKQLHLLEYMRKEYGHARS 360
                      VSK+PSELEVVLGVIKNFCK QLGKE+IAAANKQLHLLEYMRKEYGHARS
Sbjct: 924  NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 983

Query: 361  LAIAQAQVLNAHDEIKMATTRLCLKDNDDDSSADAISEHELPAASVQYSSDKFMSLAMLS 420
            LAIAQAQVLNAHDEIKMATTRLCL+ NDDD SA AISEHELPAASVQYSSDKF+SLAMLS
Sbjct: 984  LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSDKFVSLAMLS 1043

Query: 421  RVKGKLRYLKVKKFPDSFCALKFFIFRTVMSIPCLGLPSVVIIFPLDCVAILHPTYNFYY 480
            RVKGKLRYLK                                                  
Sbjct: 1044 RVKGKLRYLK-------------------------------------------------- 1103

Query: 481  FQGLVQSKQNIPVGSSSNLTLTQEPAITSTSMEQESENTLKADEESCPV----------- 540
              GLVQSKQNIP+GSSSN   TQ P ITSTS+EQ+SENTLKADEESCPV           
Sbjct: 1104 --GLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQEALSNQKMV 1163

Query: 541  ------------FPVLE------------------------------------------- 600
                        F + E                                           
Sbjct: 1164 FQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTL 1223

Query: 601  -----EHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 660
                 E E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN
Sbjct: 1224 HKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAAN 1283

Query: 661  SITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLN 676
            +ITF+RMKGGRKSHTAI EFRGQKIN KE+HKKQ   +VPELRSAQVLLLLIQHGANGLN
Sbjct: 1284 NITFVRMKGGRKSHTAISEFRGQKINTKENHKKQPLAEVPELRSAQVLLLLIQHGANGLN 1343

BLAST of Spg035427 vs. ExPASy Swiss-Prot
Match: Q149N8 (E3 ubiquitin-protein ligase SHPRH OS=Homo sapiens OX=9606 GN=SHPRH PE=1 SV=2)

HSP 1 Score: 142.5 bits (358), Expect = 1.7e-32
Identity = 159/620 (25.65%), Postives = 262/620 (42.26%), Query Frame = 0

Query: 60   LNDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLV 119
            L D  L +E + +R+ Y++  +++++ AQQ                      +  Q ++ 
Sbjct: 1090 LRDGRLEEEAKQLREHYMSKCNTEVAEAQQ--------------------ALYPVQQTIH 1149

Query: 120  ELKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSGNNSKSSRVGSRINALKYHVQ 179
            EL+ + + N  WWL  +H A E      EL++++   ++ N  +  + G    + K+   
Sbjct: 1150 ELQRKIHSNSPWWLNVIHRAIE-FTIDEELVQRVRNEITSNYKQ--QTGKLSMSEKFRDC 1209

Query: 180  TGLDLLEASRKVVLDR----LLEIDQTMENPKEED-IERVRYCQNCQADSNVPPCVLCEL 239
             GL  L  ++   L++    + E  + +E P   + IE    C    A   +  CV C+ 
Sbjct: 1210 RGLQFLLTTQMEELNKCQKLVREAVKNLEGPPSRNVIESATVCHLRPARLPLNCCVFCKA 1269

Query: 240  DELFQVSKNP--------------------------------------SELEVVLGVIKN 299
            DELF   ++                                       SE E  +  I +
Sbjct: 1270 DELFTEYESKLFSNTVKGQTAIFEEMIEDEEGLVDDRAPTTTRGLWAISETERSMKAILS 1329

Query: 300  FCKA-QLGKETIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLCLKD 359
            F K+ +   E +   +  + L E  +KEY       +A    ++A DE+ MAT RL ++D
Sbjct: 1330 FAKSHRFDVEFVDEGSTSMDLFEAWKKEYKLLHEYWMALRNRVSAVDELAMATERLRVRD 1389

Query: 360  ----NDDDSSADAISEHELPAASVQYSSDKFMSLAMLSRVKGKLRYL-KVKKFPDSFCAL 419
                  +      I  HE+    ++  +DK ++ + L +  G+L YL  ++K  D     
Sbjct: 1390 PREPKPNPPVLHIIEPHEVEQNRIKLLNDKAVATSQLQKKLGQLLYLTNLEKSQDKTSG- 1449

Query: 420  KFFIFRTVMSIPC------LGLPSVVI----IFPLDCVAILHPTYNFYYFQGLVQSKQNI 479
                   V   PC      LG    V+     F  +C++I+   Y+      +   + +I
Sbjct: 1450 ------GVNPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYS------VGSHRSSI 1509

Query: 480  PVGSSSNLTLTQEPAITSTSMEQESENTLKADEESCPVFPVLEEHETSITVQGSYGTKIE 539
                    T  +E +   TS         KA++E              I V+GS+ TK+E
Sbjct: 1510 KCAICRQTTSHKEISYVFTSE--------KANQEE------------DIPVKGSHSTKVE 1569

Query: 540  AVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANSITFIRMKGGRKSHTAIGEF-R 599
            AVVR ++ I+  D  AK LVFS+W DVLD++  A   N++ F ++   +     +  F R
Sbjct: 1570 AVVRTLMKIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKR 1629

Query: 600  GQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQ 620
              +IN                    +LLL +  G+NGL ++EA HV+LVEP+LNPA E Q
Sbjct: 1630 DPQIN--------------------ILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQ 1633

BLAST of Spg035427 vs. ExPASy Swiss-Prot
Match: Q7TPQ3 (E3 ubiquitin-protein ligase SHPRH OS=Mus musculus OX=10090 GN=Shprh PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 4.3e-31
Identity = 154/620 (24.84%), Postives = 258/620 (41.61%), Query Frame = 0

Query: 60   LNDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLV 119
            L D  L +E + +R+ Y++  +++++ AQQ                         Q S+ 
Sbjct: 1081 LRDGRLEEEAKQLREHYMSKCNTEVAEAQQ--------------------ALQPVQQSIR 1140

Query: 120  ELKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSGNNSKS------SRVGSRINA 179
            EL+ + + N  WWL  +H A E      EL++++   +S N  +       S        
Sbjct: 1141 ELQRKIHSNSPWWLNVIHRAME-FSVDEELVQRVRNEISSNYKQQTDKLSMSEKFRDCRG 1200

Query: 180  LKYHVQTGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNVPPCVLCE 239
            L++ + T ++ L   +K+V + + ++++    P  E IE    C    A   +  CV C+
Sbjct: 1201 LQFLLTTQMEELHKFQKLVREAVKKLEKP---PSREVIESATVCHLRPARLPLNCCVFCK 1260

Query: 240  LDELFQVSKNP--------------------------------------SELEVVLGVIK 299
             DELF   ++                                       SE E  +  I 
Sbjct: 1261 ADELFTEYESKLFFNTVKGQTAIFEEMIEDEEGLVDDRVPTTTRGLWAVSETERSMKAIL 1320

Query: 300  NFCKA-QLGKETIAAANKQLHLLEYMRKEYGHARSLAIAQAQVLNAHDEIKMATTRLCLK 359
            +F ++ +   E +   +  + L E  +KEY       +     ++A DE+ MAT RL ++
Sbjct: 1321 SFARSHRFDVEYVDEGSVSMDLFEAWKKEYKLLHEYWMTLRNRVSAVDELAMATERLRVR 1380

Query: 360  DNDDDSS----ADAISEHELPAASVQYSSDKFMSLAMLSRVKGKLRYL-KVKKFPDSFCA 419
               +          I  HE+    ++  +DK ++ + L +  G+L YL  ++K  D    
Sbjct: 1381 HPKEPKPNPPVHHIIEPHEVEQNRIKLVNDKAVATSQLQKKLGQLLYLTNLEKSQDKTSG 1440

Query: 420  LKFFIFRTVMSIPC------LGLPSVVI----IFPLDCVAILHPTYNFYYFQGLVQSKQN 479
                    +   PC      LG    V+     F  +C +I+   Y+      +   + +
Sbjct: 1441 -------GINPEPCPICARQLGKQWAVLTCGHCFCNECTSIIIEQYS------VGSHRSS 1500

Query: 480  IPVGSSSNLTLTQEPAITSTSMEQESENTLKADEESCPVFPVLEEHETSITVQGSYGTKI 539
            I        T  +E +   TS         KA++E              I V+GS+ TK+
Sbjct: 1501 IKCAICRQTTSHKEVSYVFTSE--------KANQED------------DIPVKGSHSTKV 1560

Query: 540  EAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANSITFIRMKGGRKSHTAIGEFR 599
            EAVVR ++ I+  D  AK LVFS+W DVLD++  A   N++ F ++   +     +  F+
Sbjct: 1561 EAVVRTLMKIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFTQISRIKTFQENLSAFK 1620

Query: 600  GQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQ 620
                   + H               +LLL +  G+NGL ++EA HV+LVEP+LNPA E Q
Sbjct: 1621 ------YDPH-------------INILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQ 1624

BLAST of Spg035427 vs. ExPASy Swiss-Prot
Match: Q9UTL9 (Uncharacterized ATP-dependent helicase C144.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC144.05 PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.8e-21
Identity = 62/156 (39.74%), Postives = 90/156 (57.69%), Query Frame = 0

Query: 467  TSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANSITFIRMK 526
            +++ ++ S+G+KI+ + + +L++K+ +   KV+VFS W DVLDVL  +F AN I FIR  
Sbjct: 1176 SNMELKESFGSKIDTISKHLLYLKHNELYPKVVVFSQWLDVLDVLHKSFEANGIVFIRF- 1235

Query: 527  GGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLNLLEAQHVV 586
             G+  +T +  F+                   E RS QVL L  +  ++GL L  A HV 
Sbjct: 1236 DGKSKNTCLKRFK-------------------EERSLQVLTLHARSQSSGLTLTNATHVF 1295

Query: 587  LVEPLLNPAAEAQAISRVHGIGQENKTFVHRFINQD 623
            + EPLLN   E QAISRVH IGQ   TFV+ +I +D
Sbjct: 1296 MCEPLLNSGIEMQAISRVHRIGQTRPTFVYYYIVED 1311

BLAST of Spg035427 vs. ExPASy Swiss-Prot
Match: Q9FIY7 (DNA repair protein RAD5B OS=Arabidopsis thaliana OX=3702 GN=RAD5B PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.0e-13
Identity = 50/179 (27.93%), Postives = 82/179 (45.81%), Query Frame = 0

Query: 455  SCPVFPVLEEHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA 514
            SCP   +       +       +K+  +++ +  IK + S  K +VFS W   LD+L+  
Sbjct: 1090 SCPTDSIF---RVDVVKNWKESSKVSELLKCLEKIKKSGSGEKSIVFSQWTSFLDLLEIP 1149

Query: 515  FAANSITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGA 574
                   F+R  G                   +  +++   +  E +   +LL+ ++ G 
Sbjct: 1150 LRRRGFEFLRFDG----------------KLAQKGREKVLKEFNETKQKTILLMSLKAGG 1209

Query: 575  NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHGIGQENKTFVHRFINQDQSFLTLQEVES 634
             GLNL  A  V L++P  NPA E QAI R+H IGQ+   FV RFI +D     +Q+V++
Sbjct: 1210 VGLNLTAASSVFLMDPWWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQVQA 1249

BLAST of Spg035427 vs. ExPASy Swiss-Prot
Match: P0CQ67 (DNA repair protein RAD5 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=RAD5 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 3.8e-11
Identity = 51/176 (28.98%), Postives = 88/176 (50.00%), Query Frame = 0

Query: 477  TKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANSITFIRMKGGRKSHTAIG 536
            TK+ A++R++  I+  D KAK LVFS +   LD+++       I ++R  G         
Sbjct: 1017 TKLRALLRQLEEIRQEDPKAKALVFSQFTSFLDLIEATLTKQGIRWLRFDGTMS------ 1076

Query: 537  EFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAA 596
              + Q+ N  E   ++ +  +       +LL+ ++ G  GLNL  A +V L++   N A 
Sbjct: 1077 --QAQRANTIEEFGRKTNEPL-------ILLISLKAGGVGLNLTMANYVFLMDTWWNEAI 1136

Query: 597  EAQAISRVHGIGQENKTFVHRFINQDQSFLTLQEVESLFASKPSPLGKSDENGSET 653
            E QAI RVH +GQ    +V R+I +      + +++    SK + +  S  NG++T
Sbjct: 1137 EQQAIDRVHRLGQNKPVYVTRYIIKGTVEKRIMKIQ---RSKTALVNASLSNGAKT 1174

BLAST of Spg035427 vs. ExPASy TrEMBL
Match: A0A6J1FKQ5 (E3 ubiquitin-protein ligase SHPRH isoform X9 OS=Cucurbita moschata OX=3662 GN=LOC111444885 PE=3 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 5.8e-249
Identity = 520/844 (61.61%), Postives = 554/844 (65.64%), Query Frame = 0

Query: 1    MDDSENYDNHITKKQKVSETLYASCSDTEKIQDNPLEPTRKDTNTKKEENYEPHWSSSFL 60
            MDDSE  DNHI KKQKV+ TLYA CSDTEKI D+PLE T  D N KKEENYEPH SSS+ 
Sbjct: 614  MDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAKKEENYEPHRSSSYF 673

Query: 61   NDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLVE 120
            ND+ LRK CEAMRQKYLAVFSSKL IAQQEFTKSYVQVC                    E
Sbjct: 674  NDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVC-------------------NE 733

Query: 121  LKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSGN-NSKSSRVGSR---INALKY 180
            LK RENLNE+WWL+AVHHAE+NKDFSHELIRKIEE VSGN N+  SRVGSR   I+ALKY
Sbjct: 734  LKSRENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSIDALKY 793

Query: 181  HVQTGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNVPPCVLCELDE 240
            HVQTGLDLLEASRK+V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSN PPCVLCELDE
Sbjct: 794  HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 853

Query: 241  LFQ--------------------------------------------------------- 300
            LFQ                                                         
Sbjct: 854  LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 913

Query: 301  ----------VSKNPSELEVVLGVIKNFCKAQLGKETIAAANKQLHLLEYMRKEYGHARS 360
                      VSK+PSELEVVLGVIKNFCK QLGKE+IAAANKQLHLLEYMRKEYGHARS
Sbjct: 914  NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 973

Query: 361  LAIAQAQVLNAHDEIKMATTRLCLKDNDDDSSADAISEHELPAASVQYSSDKFMSLAMLS 420
            LAIAQAQVLNAHDEIKMATTRLCL+ NDDD SA AISEHELPAASVQYSS+KF+SLA LS
Sbjct: 974  LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLS 1033

Query: 421  RVKGKLRYLKVKKFPDSFCALKFFIFRTVMSIPCLGLPSVVIIFPLDCVAILHPTYNFYY 480
            RVKGKLRYLK                                                  
Sbjct: 1034 RVKGKLRYLK-------------------------------------------------- 1093

Query: 481  FQGLVQSKQNIPVGSSSNLTLTQEPAITSTSMEQESENTLKADEESCPV----------- 540
              GLVQSKQNIP+GSSSN   TQ P ITSTS+EQ+SENTLKADEESCPV           
Sbjct: 1094 --GLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQETLSNQKMV 1153

Query: 541  ------------FPVLE------------------------------------------- 600
                        F + E                                           
Sbjct: 1154 FQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTL 1213

Query: 601  -----EHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 660
                 E E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN
Sbjct: 1214 HKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAAN 1273

Query: 661  SITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLN 676
            +ITF+RMKGGRKSHTAI EFRGQKINAKE+HKKQ   +VPE+RSAQVLLLLIQHGANGLN
Sbjct: 1274 NITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLIQHGANGLN 1333

BLAST of Spg035427 vs. ExPASy TrEMBL
Match: A0A6J1FF06 (E3 ubiquitin-protein ligase SHPRH isoform X7 OS=Cucurbita moschata OX=3662 GN=LOC111444885 PE=3 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 5.8e-249
Identity = 520/844 (61.61%), Postives = 554/844 (65.64%), Query Frame = 0

Query: 1    MDDSENYDNHITKKQKVSETLYASCSDTEKIQDNPLEPTRKDTNTKKEENYEPHWSSSFL 60
            MDDSE  DNHI KKQKV+ TLYA CSDTEKI D+PLE T  D N KKEENYEPH SSS+ 
Sbjct: 626  MDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAKKEENYEPHRSSSYF 685

Query: 61   NDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLVE 120
            ND+ LRK CEAMRQKYLAVFSSKL IAQQEFTKSYVQVC                    E
Sbjct: 686  NDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVC-------------------NE 745

Query: 121  LKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSGN-NSKSSRVGSR---INALKY 180
            LK RENLNE+WWL+AVHHAE+NKDFSHELIRKIEE VSGN N+  SRVGSR   I+ALKY
Sbjct: 746  LKSRENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSIDALKY 805

Query: 181  HVQTGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNVPPCVLCELDE 240
            HVQTGLDLLEASRK+V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSN PPCVLCELDE
Sbjct: 806  HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 865

Query: 241  LFQ--------------------------------------------------------- 300
            LFQ                                                         
Sbjct: 866  LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 925

Query: 301  ----------VSKNPSELEVVLGVIKNFCKAQLGKETIAAANKQLHLLEYMRKEYGHARS 360
                      VSK+PSELEVVLGVIKNFCK QLGKE+IAAANKQLHLLEYMRKEYGHARS
Sbjct: 926  NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 985

Query: 361  LAIAQAQVLNAHDEIKMATTRLCLKDNDDDSSADAISEHELPAASVQYSSDKFMSLAMLS 420
            LAIAQAQVLNAHDEIKMATTRLCL+ NDDD SA AISEHELPAASVQYSS+KF+SLA LS
Sbjct: 986  LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLS 1045

Query: 421  RVKGKLRYLKVKKFPDSFCALKFFIFRTVMSIPCLGLPSVVIIFPLDCVAILHPTYNFYY 480
            RVKGKLRYLK                                                  
Sbjct: 1046 RVKGKLRYLK-------------------------------------------------- 1105

Query: 481  FQGLVQSKQNIPVGSSSNLTLTQEPAITSTSMEQESENTLKADEESCPV----------- 540
              GLVQSKQNIP+GSSSN   TQ P ITSTS+EQ+SENTLKADEESCPV           
Sbjct: 1106 --GLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQETLSNQKMV 1165

Query: 541  ------------FPVLE------------------------------------------- 600
                        F + E                                           
Sbjct: 1166 FQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTL 1225

Query: 601  -----EHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 660
                 E E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN
Sbjct: 1226 HKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAAN 1285

Query: 661  SITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLN 676
            +ITF+RMKGGRKSHTAI EFRGQKINAKE+HKKQ   +VPE+RSAQVLLLLIQHGANGLN
Sbjct: 1286 NITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLIQHGANGLN 1345

BLAST of Spg035427 vs. ExPASy TrEMBL
Match: A0A6J1FE16 (E3 ubiquitin-protein ligase SHPRH isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444885 PE=3 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 5.8e-249
Identity = 520/844 (61.61%), Postives = 554/844 (65.64%), Query Frame = 0

Query: 1    MDDSENYDNHITKKQKVSETLYASCSDTEKIQDNPLEPTRKDTNTKKEENYEPHWSSSFL 60
            MDDSE  DNHI KKQKV+ TLYA CSDTEKI D+PLE T  D N KKEENYEPH SSS+ 
Sbjct: 915  MDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAKKEENYEPHRSSSYF 974

Query: 61   NDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLVE 120
            ND+ LRK CEAMRQKYLAVFSSKL IAQQEFTKSYVQVC                    E
Sbjct: 975  NDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVC-------------------NE 1034

Query: 121  LKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSGN-NSKSSRVGSR---INALKY 180
            LK RENLNE+WWL+AVHHAE+NKDFSHELIRKIEE VSGN N+  SRVGSR   I+ALKY
Sbjct: 1035 LKSRENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSIDALKY 1094

Query: 181  HVQTGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNVPPCVLCELDE 240
            HVQTGLDLLEASRK+V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSN PPCVLCELDE
Sbjct: 1095 HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 1154

Query: 241  LFQ--------------------------------------------------------- 300
            LFQ                                                         
Sbjct: 1155 LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 1214

Query: 301  ----------VSKNPSELEVVLGVIKNFCKAQLGKETIAAANKQLHLLEYMRKEYGHARS 360
                      VSK+PSELEVVLGVIKNFCK QLGKE+IAAANKQLHLLEYMRKEYGHARS
Sbjct: 1215 NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1274

Query: 361  LAIAQAQVLNAHDEIKMATTRLCLKDNDDDSSADAISEHELPAASVQYSSDKFMSLAMLS 420
            LAIAQAQVLNAHDEIKMATTRLCL+ NDDD SA AISEHELPAASVQYSS+KF+SLA LS
Sbjct: 1275 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLS 1334

Query: 421  RVKGKLRYLKVKKFPDSFCALKFFIFRTVMSIPCLGLPSVVIIFPLDCVAILHPTYNFYY 480
            RVKGKLRYLK                                                  
Sbjct: 1335 RVKGKLRYLK-------------------------------------------------- 1394

Query: 481  FQGLVQSKQNIPVGSSSNLTLTQEPAITSTSMEQESENTLKADEESCPV----------- 540
              GLVQSKQNIP+GSSSN   TQ P ITSTS+EQ+SENTLKADEESCPV           
Sbjct: 1395 --GLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQETLSNQKMV 1454

Query: 541  ------------FPVLE------------------------------------------- 600
                        F + E                                           
Sbjct: 1455 FQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTL 1514

Query: 601  -----EHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 660
                 E E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN
Sbjct: 1515 HKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAAN 1574

Query: 661  SITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLN 676
            +ITF+RMKGGRKSHTAI EFRGQKINAKE+HKKQ   +VPE+RSAQVLLLLIQHGANGLN
Sbjct: 1575 NITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLIQHGANGLN 1634

BLAST of Spg035427 vs. ExPASy TrEMBL
Match: A0A6J1FJU8 (E3 ubiquitin-protein ligase SHPRH isoform X8 OS=Cucurbita moschata OX=3662 GN=LOC111444885 PE=3 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 5.8e-249
Identity = 520/844 (61.61%), Postives = 554/844 (65.64%), Query Frame = 0

Query: 1    MDDSENYDNHITKKQKVSETLYASCSDTEKIQDNPLEPTRKDTNTKKEENYEPHWSSSFL 60
            MDDSE  DNHI KKQKV+ TLYA CSDTEKI D+PLE T  D N KKEENYEPH SSS+ 
Sbjct: 624  MDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAKKEENYEPHRSSSYF 683

Query: 61   NDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLVE 120
            ND+ LRK CEAMRQKYLAVFSSKL IAQQEFTKSYVQVC                    E
Sbjct: 684  NDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVC-------------------NE 743

Query: 121  LKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSGN-NSKSSRVGSR---INALKY 180
            LK RENLNE+WWL+AVHHAE+NKDFSHELIRKIEE VSGN N+  SRVGSR   I+ALKY
Sbjct: 744  LKSRENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSIDALKY 803

Query: 181  HVQTGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNVPPCVLCELDE 240
            HVQTGLDLLEASRK+V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSN PPCVLCELDE
Sbjct: 804  HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 863

Query: 241  LFQ--------------------------------------------------------- 300
            LFQ                                                         
Sbjct: 864  LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 923

Query: 301  ----------VSKNPSELEVVLGVIKNFCKAQLGKETIAAANKQLHLLEYMRKEYGHARS 360
                      VSK+PSELEVVLGVIKNFCK QLGKE+IAAANKQLHLLEYMRKEYGHARS
Sbjct: 924  NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 983

Query: 361  LAIAQAQVLNAHDEIKMATTRLCLKDNDDDSSADAISEHELPAASVQYSSDKFMSLAMLS 420
            LAIAQAQVLNAHDEIKMATTRLCL+ NDDD SA AISEHELPAASVQYSS+KF+SLA LS
Sbjct: 984  LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLS 1043

Query: 421  RVKGKLRYLKVKKFPDSFCALKFFIFRTVMSIPCLGLPSVVIIFPLDCVAILHPTYNFYY 480
            RVKGKLRYLK                                                  
Sbjct: 1044 RVKGKLRYLK-------------------------------------------------- 1103

Query: 481  FQGLVQSKQNIPVGSSSNLTLTQEPAITSTSMEQESENTLKADEESCPV----------- 540
              GLVQSKQNIP+GSSSN   TQ P ITSTS+EQ+SENTLKADEESCPV           
Sbjct: 1104 --GLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQETLSNQKMV 1163

Query: 541  ------------FPVLE------------------------------------------- 600
                        F + E                                           
Sbjct: 1164 FQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTL 1223

Query: 601  -----EHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 660
                 E E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN
Sbjct: 1224 HKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAAN 1283

Query: 661  SITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLN 676
            +ITF+RMKGGRKSHTAI EFRGQKINAKE+HKKQ   +VPE+RSAQVLLLLIQHGANGLN
Sbjct: 1284 NITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLIQHGANGLN 1343

BLAST of Spg035427 vs. ExPASy TrEMBL
Match: A0A6J1FKQ1 (E3 ubiquitin-protein ligase SHPRH isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111444885 PE=3 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 5.8e-249
Identity = 520/844 (61.61%), Postives = 554/844 (65.64%), Query Frame = 0

Query: 1    MDDSENYDNHITKKQKVSETLYASCSDTEKIQDNPLEPTRKDTNTKKEENYEPHWSSSFL 60
            MDDSE  DNHI KKQKV+ TLYA CSDTEKI D+PLE T  D N KKEENYEPH SSS+ 
Sbjct: 667  MDDSEKSDNHIIKKQKVNGTLYAPCSDTEKIIDSPLELTGLDENAKKEENYEPHRSSSYF 726

Query: 61   NDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLVE 120
            ND+ LRK CEAMRQKYLAVFSSKL IAQQEFTKSYVQVC                    E
Sbjct: 727  NDNYLRKACEAMRQKYLAVFSSKLCIAQQEFTKSYVQVC-------------------NE 786

Query: 121  LKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSGN-NSKSSRVGSR---INALKY 180
            LK RENLNE+WWL+AVHHAE+NKDFSHELIRKIEE VSGN N+  SRVGSR   I+ALKY
Sbjct: 787  LKSRENLNEVWWLQAVHHAEQNKDFSHELIRKIEEAVSGNLNNSKSRVGSRFRSIDALKY 846

Query: 181  HVQTGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNVPPCVLCELDE 240
            HVQTGLDLLEASRK+V+ RLLEIDQTME+PK+EDIERVRYCQNCQADSN PPCVLCELDE
Sbjct: 847  HVQTGLDLLEASRKLVIGRLLEIDQTMESPKDEDIERVRYCQNCQADSNGPPCVLCELDE 906

Query: 241  LFQ--------------------------------------------------------- 300
            LFQ                                                         
Sbjct: 907  LFQEYEARLFRLNTVQGGMVTSVEEAVEAQKKKSALNRFYWSLLQQNKNLSSSRDGYEES 966

Query: 301  ----------VSKNPSELEVVLGVIKNFCKAQLGKETIAAANKQLHLLEYMRKEYGHARS 360
                      VSK+PSELEVVLGVIKNFCK QLGKE+IAAANKQLHLLEYMRKEYGHARS
Sbjct: 967  NKRDAGERVVVSKHPSELEVVLGVIKNFCKTQLGKESIAAANKQLHLLEYMRKEYGHARS 1026

Query: 361  LAIAQAQVLNAHDEIKMATTRLCLKDNDDDSSADAISEHELPAASVQYSSDKFMSLAMLS 420
            LAIAQAQVLNAHDEIKMATTRLCL+ NDDD SA AISEHELPAASVQYSS+KF+SLA LS
Sbjct: 1027 LAIAQAQVLNAHDEIKMATTRLCLRGNDDDDSAYAISEHELPAASVQYSSNKFVSLATLS 1086

Query: 421  RVKGKLRYLKVKKFPDSFCALKFFIFRTVMSIPCLGLPSVVIIFPLDCVAILHPTYNFYY 480
            RVKGKLRYLK                                                  
Sbjct: 1087 RVKGKLRYLK-------------------------------------------------- 1146

Query: 481  FQGLVQSKQNIPVGSSSNLTLTQEPAITSTSMEQESENTLKADEESCPV----------- 540
              GLVQSKQNIP+GSSSN   TQ P ITSTS+EQ+SENTLKADEESCPV           
Sbjct: 1147 --GLVQSKQNIPLGSSSNPASTQVPDITSTSVEQKSENTLKADEESCPVCQETLSNQKMV 1206

Query: 541  ------------FPVLE------------------------------------------- 600
                        F + E                                           
Sbjct: 1207 FQCGHMTCCKCLFAMTEKKLRDSTIQTKWVMCPTCRQHTDFGNIAYADDSKNETLDPSTL 1266

Query: 601  -----EHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAAN 660
                 E E SI VQGSYGTKIEAVVRRILWIKYTDS+AKVLVFSSWNDVLDVLQYAFAAN
Sbjct: 1267 HKTSREDERSIRVQGSYGTKIEAVVRRILWIKYTDSEAKVLVFSSWNDVLDVLQYAFAAN 1326

Query: 661  SITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLN 676
            +ITF+RMKGGRKSHTAI EFRGQKINAKE+HKKQ   +VPE+RSAQVLLLLIQHGANGLN
Sbjct: 1327 NITFVRMKGGRKSHTAISEFRGQKINAKENHKKQPLAEVPEIRSAQVLLLLIQHGANGLN 1386

BLAST of Spg035427 vs. TAIR 10
Match: AT2G40770.1 (zinc ion binding;DNA binding;helicases;ATP binding;nucleic acid binding )

HSP 1 Score: 450.3 bits (1157), Expect = 2.8e-126
Identity = 322/798 (40.35%), Postives = 423/798 (53.01%), Query Frame = 0

Query: 3    DSENYDNH-ITKKQKVSETLYASCSDTEKIQDNPLEPTRKDTNTKKE-ENYEPHWSSSFL 62
            D ++ D+H  +K+Q+++E    +   +E +     E    D   KK+ E +E        
Sbjct: 897  DVKDDDHHRASKRQRINELESLTHDSSETVHQR--EAIAPDNGLKKDGECHE-------- 956

Query: 63   NDSSLRKECEAMRQKYLAVFSSKLSIAQQEFTKSYVQVCFYVLLLGGYNVYHHSQYSLVE 122
               +L   C+ ++ KYL+ F+SKLS AQ EF KSY QV                      
Sbjct: 957  ECKTLDIVCDTLKVKYLSAFNSKLSAAQHEFKKSYNQVS-------------------ES 1016

Query: 123  LKDRENLNEIWWLEAVHHAEENKDFSHELIRKIEEVVSG--NNSKSSRVGSR---INALK 182
            L +      +WWL+A+   E+NKDFS EL RKIEE + G  NNS SSR  SR   I+ +K
Sbjct: 1017 LSNMGKQRSVWWLDALQLTEQNKDFSSELTRKIEEALHGNLNNSSSSRESSRFRTIHGMK 1076

Query: 183  YHVQTGLDLLEASRKVVLDRLLEIDQTMENPKEEDIERVRYCQNCQADSNVPPCVLCELD 242
             H+QT +D+LE SRK V+DR+LEIDQTME PK EDIER+  C+ C  +S+ PPC+ CELD
Sbjct: 1077 LHLQTCMDMLERSRKKVIDRILEIDQTMEKPKLEDIERISNCKYCNKNSDGPPCIHCELD 1136

Query: 243  ELFQ-------------------------------------------------------- 302
            ELFQ                                                        
Sbjct: 1137 ELFQEYEARLFRLNKSRRGVMEIAAAEETVHLQKKRDARNLFLFGLSSRSKDLNASRGDD 1196

Query: 303  -------------VSKNPSELEVVLGVIKNFCKAQLGKETIAAANKQLHLLEYMRKEYGH 362
                         +SK+PSE E+VLGVI+N CK  L +E+  AA K LH LE MRKEY H
Sbjct: 1197 EEPTKRNAGDIVVLSKSPSETEIVLGVIRNHCKTHLDRESKLAATKHLHTLEVMRKEYVH 1256

Query: 363  ARSLAIAQAQVLNAHDEIKMATTRLCLKDNDDDSSADAISEHELPAASVQYSSDKFMSLA 422
            AR LA  QAQ+L A+DEI M+T RL L++++DD+S  A+   EL  ASV  ++DKFM+ +
Sbjct: 1257 ARVLARDQAQLLRAYDEINMSTMRLQLRESEDDTSIYALGRDELDVASVLNTNDKFMAQS 1316

Query: 423  MLSRVKGKLRYLK-----VKKFPDSFCALKFFIFRTVMSIPCLGLPSVVIIFPLDCVAIL 482
             L  +KGKLRYLK      +K       L   I  TV +       S  ++   +   I 
Sbjct: 1317 SLLSIKGKLRYLKGLMKSKQKQESESPDLSSPIHETVDASDPAEQESENLLKRDEACPIC 1376

Query: 483  HPTY---------------NFYYFQGLVQSKQNIPVGSSSNLTLTQEPAITSTSMEQESE 542
            H                  N ++     +S Q             Q   + + +   +  
Sbjct: 1377 HEILRNQKMVFQCGHSTCCNCFFAMTERKSVQETLQKWVMCPICRQHTDVRNIAYADDRR 1436

Query: 543  NTLKADEESCPVFPVLEEHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWND 602
            N+  +D++        ++ E S+ VQGSYGTKIEAV RRILWIK +D + KVLVFSSWND
Sbjct: 1437 NSSSSDQDH-------KDSEASLVVQGSYGTKIEAVTRRILWIKSSDPQTKVLVFSSWND 1496

Query: 603  VLDVLQYAFAANSITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVL 662
            VLDVL++AFAANSIT IRMKGGRKS TAI +F+G      E   ++ ++   E +S QVL
Sbjct: 1497 VLDVLEHAFAANSITCIRMKGGRKSQTAISKFKG-----SEKETQKTNSHQKEEKSIQVL 1556

Query: 663  LLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHGIGQENKTFVHRFI------- 673
            LLL+QHGANGLNLLEAQHV+LVEPLLNPAAEAQA+ RVH IGQE  T VHRF+       
Sbjct: 1557 LLLVQHGANGLNLLEAQHVILVEPLLNPAAEAQAVGRVHRIGQEKPTLVHRFLVSGTVEE 1616

BLAST of Spg035427 vs. TAIR 10
Match: AT5G43530.1 (Helicase protein with RING/U-box domain )

HSP 1 Score: 78.2 bits (191), Expect = 2.9e-14
Identity = 50/179 (27.93%), Postives = 82/179 (45.81%), Query Frame = 0

Query: 455  SCPVFPVLEEHETSITVQGSYGTKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYA 514
            SCP   +       +       +K+  +++ +  IK + S  K +VFS W   LD+L+  
Sbjct: 1090 SCPTDSIF---RVDVVKNWKESSKVSELLKCLEKIKKSGSGEKSIVFSQWTSFLDLLEIP 1149

Query: 515  FAANSITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGA 574
                   F+R  G                   +  +++   +  E +   +LL+ ++ G 
Sbjct: 1150 LRRRGFEFLRFDG----------------KLAQKGREKVLKEFNETKQKTILLMSLKAGG 1209

Query: 575  NGLNLLEAQHVVLVEPLLNPAAEAQAISRVHGIGQENKTFVHRFINQDQSFLTLQEVES 634
             GLNL  A  V L++P  NPA E QAI R+H IGQ+   FV RFI +D     +Q+V++
Sbjct: 1210 VGLNLTAASSVFLMDPWWNPAVEEQAIMRIHRIGQKRTVFVRRFIVKDTVEERMQQVQA 1249

BLAST of Spg035427 vs. TAIR 10
Match: AT1G02670.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 1.4e-13
Identity = 68/215 (31.63%), Postives = 104/215 (48.37%), Query Frame = 0

Query: 438 STSMEQESENTLKADEESCPVFPV-LEEHETSITVQGSYGTKIEAVVRRILWIKYTDSKA 497
           ++S  Q S+  LK    S  +  + L++ +TS        TKIEA+   I ++   D  A
Sbjct: 475 TSSEHQASKTKLKGFRASSILNRINLDDFKTS--------TKIEALREEIRFMVERDWSA 534

Query: 498 KVLVFSSWNDVLDVLQYAFAANSITFIRMKGGRKSHTAIGEFRGQKINAKESHKKQASTQ 557
           K +VFS +   LD++ YA   + ++ +++ G                +  ++ K  A   
Sbjct: 535 KAIVFSQFTSFLDLISYALGKSGVSCVQLVG----------------SMSKAAKDAALKN 594

Query: 558 VPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVHGIGQENKTFVH 617
             E    +VLL+ +Q G   LNL  A HV +++P  NPA E QA  R+H IGQ     V 
Sbjct: 595 FKEEPDCRVLLMSLQAGGVALNLTAASHVFMMDPWWNPAVERQAQDRIHRIGQCKPVRVV 654

Query: 618 RFINQ---DQSFLTLQ-EVESLFASKPSPLGKSDE 648
           RFI +   ++  LTLQ + E LF    S LG S+E
Sbjct: 655 RFIMEKTVEEKILTLQKKKEDLF---ESTLGDSEE 662

BLAST of Spg035427 vs. TAIR 10
Match: AT5G22750.1 (DNA/RNA helicase protein )

HSP 1 Score: 69.3 bits (168), Expect = 1.3e-11
Identity = 41/143 (28.67%), Postives = 74/143 (51.75%), Query Frame = 0

Query: 477 TKIEAVVRRILWIKYTDSKAKVLVFSSWNDVLDVLQYAFAANSITFIRMKGGRKSHTAIG 536
           +KI A++  +  ++ + SK+  ++FS W   LD+LQ   + N+ +F+R+ G         
Sbjct: 863 SKITALLEELEGLRSSGSKS--ILFSQWTAFLDLLQIPLSRNNFSFVRLDG--------- 922

Query: 537 EFRGQKINAKESHKKQASTQVPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAA 596
                     +  +++   +  E  S  VLL+ ++ G  G+NL  A +  +++P  NPA 
Sbjct: 923 -------TLSQQQREKVLKEFSEDGSILVLLMSLKAGGVGINLTAASNAFVMDPWWNPAV 982

Query: 597 EAQAISRVHGIGQENKTFVHRFI 620
           E QA+ R+H IGQ  +  + RFI
Sbjct: 983 EEQAVMRIHRIGQTKEVKIRRFI 987

BLAST of Spg035427 vs. TAIR 10
Match: AT1G05120.1 (Helicase protein with RING/U-box domain )

HSP 1 Score: 66.2 bits (160), Expect = 1.1e-10
Identity = 61/212 (28.77%), Postives = 99/212 (46.70%), Query Frame = 0

Query: 430 LTQEPAITSTSMEQESENTLKADEESCPVFPV-LEEHETSITVQGSYGTKIEAVVRRILW 489
           LT +    + +  + S+ TLK    S  +  + L++ +TS        TKIEA+   I +
Sbjct: 622 LTVDWTTKADTEHKASKTTLKGFRASSILNRIKLDDFQTS--------TKIEALREEIRF 681

Query: 490 IKYTDSKAKVLVFSSWNDVLDVLQYAFAANSITFIRMKGG---RKSHTAIGEFRGQKINA 549
           +   D  AK +VFS +   LD++ Y      ++ +++ G        TAI +F+      
Sbjct: 682 MVERDGSAKAIVFSQFTSFLDLINYTLGKCGVSCVQLVGSMTMAARDTAINKFK------ 741

Query: 550 KESHKKQASTQVPELRSAQVLLLLIQHGANGLNLLEAQHVVLVEPLLNPAAEAQAISRVH 609
                     + P+ R   V L+ ++ G   LNL  A HV +++P  NPA E QA  R+H
Sbjct: 742 ----------EDPDCR---VFLMSLKAGGVALNLTVASHVFMMDPWWNPAVERQAQDRIH 801

Query: 610 GIGQENKTFVHRFINQ---DQSFLTLQEVESL 635
            IGQ     V RFI +   ++  L LQ+ + L
Sbjct: 802 RIGQYKPIRVVRFIIENTVEERILRLQKKKEL 806