Spg035355 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg035355
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Proteinase inhibitor 1
Location	scaffold7: 22732239 .. 22732826 (+)
RNA-Seq Expression	Spg035355
Synteny	Spg035355

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTAATATTATTTTTTAAAACAGTGTGCATCGGCCAAATTGGGTATAAATATTGGTAGCTGAGACATTAACAAAGCATCCCCAATCGACCATCAGCTAAGAAAACAAAGAAGACAGTGTGAGAGTTTTGAGATGTCTCATTCTACAGGTTTGCAAACCTCTCCTTTCAAACCTCATGCATTTCAAATGCTTCTTATTTTCCATGGCAATTTTGTTTATTTATTTCTTTTACTACTTTGGATTTAATGTGTGTCATTCTATGATTCAAATTAGGAAAACGACAATGGCCAGAACTTGTCGGTGTTCCAGCCGACATTGCAAAGCTTATCATAGGAAAAGACAATCCTGCTATAGATCAAATACCGGTATTATTAGCAGGATCTCCAGTAACTCGGGATCACAGAGAGGATCGAGTTCGAATATTTGTTAATACAATCTATATTTGTGTTATTCCTCCTGTAGTTGGTTGAAATGAGATATGAATATCTATGTTTGAACGGGTTAAGATATTAATAAAATAAGGTAGAATAATCTTTTTCTACCCTTTAAATGATTTGTGTAAGATCTGTTTTTAATTTAAAATATTATT

mRNA sequence

ATGTCTCATTCTACAGGAAAACGACAATGGCCAGAACTTGTCGGTGTTCCAGCCGACATTGCAAAGCTTATCATAGGAAAAGACAATCCTGCTATAGATCAAATACCGGTATTATTAGCAGGATCTCCAGTAACTCGGGATCACAGAGAGGATCGAGTTCGAATATTTGTTAATACAATCTATATTTGTGTTATTCCTCCTGTAGTTGGTTGA

Coding sequence (CDS)

ATGTCTCATTCTACAGGAAAACGACAATGGCCAGAACTTGTCGGTGTTCCAGCCGACATTGCAAAGCTTATCATAGGAAAAGACAATCCTGCTATAGATCAAATACCGGTATTATTAGCAGGATCTCCAGTAACTCGGGATCACAGAGAGGATCGAGTTCGAATATTTGTTAATACAATCTATATTTGTGTTATTCCTCCTGTAGTTGGTTGA

Protein sequence

MSHSTGKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTIYICVIPPVVG

Homology

[Close]

BLAST of Spg035355 vs. NCBI nr
Match: XP_004247655.1 (wound-induced proteinase inhibitor 1-like [Solanum lycopersicum])

HSP 1 Score: 79.3 bits (194), Expect = 1.4e-11
Identity = 39/64 (60.94%), Postives = 48/64 (75.00%), Query Frame = 0


Query: 6   GKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTI-YICV 65
           GK+ WPEL+GVPA +AK II K+NP+I  IP+LL GSPVT D R DRVR+F N + Y+  
Sbjct: 47  GKQFWPELIGVPAKLAKEIIEKENPSISDIPILLNGSPVTMDFRCDRVRLFDNILGYVVQ 106

Query: 66  IPPV 69
           IP V
Sbjct: 107 IPTV 110

[Close]

BLAST of Spg035355 vs. NCBI nr
Match: TMW85239.1 (hypothetical protein EJD97_023458 [Solanum chilense])

HSP 1 Score: 79.3 bits (194), Expect = 1.4e-11
Identity = 40/68 (58.82%), Postives = 49/68 (72.06%), Query Frame = 0


Query: 2   SHSTGKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTIY 61
           S   GK+ WPEL+GVPA  AK II K+NP+I  IP+LL GSPVT+D R DRVR+FVN + 
Sbjct: 36  SRCKGKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILG 95

Query: 62  ICVIPPVV 70
           + V  P V
Sbjct: 96  VVVQIPRV 103

[Close]

BLAST of Spg035355 vs. NCBI nr
Match: P16231.1 (RecName: Full=Wound-induced proteinase inhibitor 1; AltName: Full=Wound-induced proteinase inhibitor I; Flags: Precursor [Solanum peruvianum] >AAA34180.1 proteinase inhibitor I precursor [Solanum peruvianum])

HSP 1 Score: 78.2 bits (191), Expect = 3.2e-11
Identity = 37/59 (62.71%), Postives = 45/59 (76.27%), Query Frame = 0


Query: 2   SHSTGKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTI 61
           S   GK+ WPEL+GVPA  AK II K+NP+I  IP+LL GSPVT+D R DRVR+FVN +
Sbjct: 43  SRCKGKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNIL 101

[Close]

BLAST of Spg035355 vs. NCBI nr
Match: NP_001315434.1 (proteinase inhibitor I precursor [Solanum lycopersicum])

HSP 1 Score: 78.2 bits (191), Expect = 3.2e-11
Identity = 36/55 (65.45%), Postives = 44/55 (80.00%), Query Frame = 0


Query: 6   GKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTI 61
           GK+ WPEL+GVPA  AK II K+NP+I  IP+LL GSPVT+D R DRVR+FVN +
Sbjct: 47  GKQFWPELIGVPAQYAKGIIEKENPSIANIPILLNGSPVTKDFRCDRVRLFVNIL 101

[Close]

BLAST of Spg035355 vs. NCBI nr
Match: AAA34198.1 (proteinase inhibitor I [Solanum peruvianum])

HSP 1 Score: 78.2 bits (191), Expect = 3.2e-11
Identity = 37/59 (62.71%), Postives = 45/59 (76.27%), Query Frame = 0


Query: 2   SHSTGKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTI 61
           S   GK+ WPEL+GVPA  AK II K+NP+I  IP+LL GSPVT+D R DRVR+FVN +
Sbjct: 43  SRCKGKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNIL 101

[Close]

BLAST of Spg035355 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.2e-14
Identity = 37/59 (62.71%), Postives = 45/59 (76.27%), Query Frame = 0


Query: 2   SHSTGKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTI 61
           S   GK+ WPEL+GVPA  AK II K+NP+I  IP+LL GSPVT+D R DRVR+FVN +
Sbjct: 43  SRCKGKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNIL 101

[Close]

BLAST of Spg035355 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 7.1e-14
Identity = 36/64 (56.25%), Postives = 47/64 (73.44%), Query Frame = 0


Query: 6   GKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTIYICVI 65
           GK+ WPEL+GVP  +AK II K+NP+I  IP+LL+GSP+T D+  DRVR+F N +   V 
Sbjct: 47  GKQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQ 106

Query: 66  PPVV 70
            PVV
Sbjct: 107 MPVV 110

[Close]

BLAST of Spg035355 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 6.6e-12
Identity = 36/65 (55.38%), Postives = 44/65 (67.69%), Query Frame = 0


Query: 6   GKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTIYICVI 65
           GK +WPEL+GVP  +AK II K+N  I  + +LL GSPVT D R DRVR+F N +   V 
Sbjct: 43  GKLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILGYVVD 102

Query: 66  PPVVG 71
            PVVG
Sbjct: 103 IPVVG 107

[Close]

BLAST of Spg035355 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.9e-11
Identity = 33/64 (51.56%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 7  KRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTIYICVIP 66
          K  WPEL+GVPA  A+ II K+N  +  +P +L GSPVT+D R +RVR+FVN +   V  
Sbjct: 31 KETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQI 90

Query: 67 PVVG 71
          P VG
Sbjct: 91 PRVG 94

[Close]

BLAST of Spg035355 vs. ExPASy Swiss-Prot
Match: P08454 (Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 7.3e-11
Identity = 30/55 (54.55%), Postives = 41/55 (74.55%), Query Frame = 0


Query: 6  GKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTI 61
          GK++WPEL+GVP  +AK II K+N  I  + +LL GSPVT D+R +RVR+F N +
Sbjct: 43 GKQRWPELIGVPTKLAKGIIEKENSLITNVQILLNGSPVTMDYRCNRVRLFDNIL 97

[Close]

BLAST of Spg035355 vs. ExPASy TrEMBL
Match: A0A3Q7J2Q4 (Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101247257 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 6.9e-12
Identity = 39/64 (60.94%), Postives = 48/64 (75.00%), Query Frame = 0


Query: 6   GKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTI-YICV 65
           GK+ WPEL+GVPA +AK II K+NP+I  IP+LL GSPVT D R DRVR+F N + Y+  
Sbjct: 47  GKQFWPELIGVPAKLAKEIIEKENPSISDIPILLNGSPVTMDFRCDRVRLFDNILGYVVQ 106

Query: 66  IPPV 69
           IP V
Sbjct: 107 IPTV 110

[Close]

BLAST of Spg035355 vs. ExPASy TrEMBL
Match: A0A6N2AU12 (Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023458 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 6.9e-12
Identity = 40/68 (58.82%), Postives = 49/68 (72.06%), Query Frame = 0


Query: 2   SHSTGKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTIY 61
           S   GK+ WPEL+GVPA  AK II K+NP+I  IP+LL GSPVT+D R DRVR+FVN + 
Sbjct: 36  SRCKGKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILG 95

Query: 62  ICVIPPVV 70
           + V  P V
Sbjct: 96  VVVQIPRV 103

[Close]

BLAST of Spg035355 vs. ExPASy TrEMBL
Match: A0A3Q7I868 (Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101247557 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.5e-11
Identity = 36/55 (65.45%), Postives = 44/55 (80.00%), Query Frame = 0


Query: 6   GKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTI 61
           GK+ WPEL+GVPA  AK II K+NP+I  IP+LL GSPVT+D R DRVR+FVN +
Sbjct: 47  GKQFWPELIGVPAQYAKGIIEKENPSIANIPILLNGSPVTKDFRCDRVRLFVNIL 101

[Close]

BLAST of Spg035355 vs. ExPASy TrEMBL
Match: A0A6N2B1Z1 (Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_018438 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.6e-11
Identity = 39/64 (60.94%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 6   GKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTIYICVI 65
           GK++WPEL+GVPA  AK II K+NP I  IP+LL GSPVT D R DRVR+F N +   V 
Sbjct: 47  GKQRWPELIGVPAQYAKGIIEKENPFIADIPILLNGSPVTMDFRCDRVRLFDNILGYVVQ 106

Query: 66  PPVV 70
            PVV
Sbjct: 107 MPVV 110

[Close]

BLAST of Spg035355 vs. ExPASy TrEMBL
Match: A0A6N2AU19 (Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023457 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 7.6e-11
Identity = 37/65 (56.92%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 6   GKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTIYICVI 65
           GK+ WPELVGVP  +AK II K+NP+I  IP+L  GSP+T D R +RVR+F N +   V 
Sbjct: 47  GKQFWPELVGVPTKLAKKIIEKENPSISDIPILFNGSPLTMDFRCNRVRLFDNILGYVVQ 106

Query: 66  PPVVG 71
            PVVG
Sbjct: 107 IPVVG 111

[Close]

BLAST of Spg035355 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 58.2 bits (139), Expect = 3.2e-09
Identity = 30/64 (46.88%), Postives = 39/64 (60.94%), Query Frame = 0


Query: 7  KRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTIYICVIP 66
          K  WPEL G   D A ++I ++NP ++   V+L GSPVT D R DRVR+FV+   I V  
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVN-AAVILDGSPVTADFRCDRVRVFVDGNRIVVKT 67

Query: 67 PVVG 71
          P  G
Sbjct: 68 PKSG 70

[Close]

BLAST of Spg035355 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 44.3 bits (103), Expect = 4.7e-05
Identity = 23/65 (35.38%), Postives = 37/65 (56.92%), Query Frame = 0


Query: 6  GKRQWPELVGVPADIAKLIIGKDNPAIDQIPVLLAGSPVTRDHREDRVRIFVNTIYICVI 65
          GK  WPEL+G   + AK +I ++NP +  + ++L G+ V       RV ++VN   I V 
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAV-IILDGTVVPEIFICSRVYVWVNDCGIVVQ 95

Query: 66 PPVVG 71
           P++G
Sbjct: 96 IPIIG 99

The following BLAST results are available for this feature:

BLAST of Spg035355 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004247655.1	1.4e-11	60.94	wound-induced proteinase inhibitor 1-like [Solanum lycopersicum]	[more]
TMW85239.1	1.4e-11	58.82	hypothetical protein EJD97_023458 [Solanum chilense]	[more]
P16231.1	3.2e-11	62.71	RecName: Full=Wound-induced proteinase inhibitor 1; AltName: Full=Wound-induced ...	[more]
NP_001315434.1	3.2e-11	65.45	proteinase inhibitor I precursor [Solanum lycopersicum]	[more]
AAA34198.1	3.2e-11	62.71	proteinase inhibitor I [Solanum peruvianum]	[more]

BLAST of Spg035355 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P16231	4.2e-14	62.71	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P05118	7.1e-14	56.25	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]
Q00783	6.6e-12	55.38	Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1	[more]
Q02214	1.9e-11	51.56	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
P08454	7.3e-11	54.55	Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2	[more]

BLAST of Spg035355 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A3Q7J2Q4	6.9e-12	60.94	Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101247257 PE=3 SV=1	[more]
A0A6N2AU12	6.9e-12	58.82	Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023458 PE=3 SV=1	[more]
A0A3Q7I868	1.5e-11	65.45	Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101247557 PE=3 SV=1	[more]
A0A6N2B1Z1	2.6e-11	60.94	Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_018438 PE=3 SV=1	[more]
A0A6N2AU19	7.6e-11	56.92	Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023457 PE=3 SV=1	[more]

BLAST of Spg035355 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	3.2e-09	46.88	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	4.7e-05	35.38	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 9..22 score: 59.94 coord: 35..46 score: 47.83 coord: 47..57 score: 56.13
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 7..70 e-value: 8.0E-20 score: 71.3
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 4..70
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 10..21
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..70 e-value: 1.4E-19 score: 72.0
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 4..70
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 3..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg035355.1	Spg035355.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity