Spg035353 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg035353 vs. NCBI nr
Match: XP_038876924.1 (uncharacterized protein LOC120069278 [Benincasa hispida] >XP_038876925.1 uncharacterized protein LOC120069278 [Benincasa hispida])

HSP 1 Score: 2020.7 bits (5234), Expect = 0.0e+00
Identity = 1041/1197 (86.97%), Postives = 1093/1197 (91.31%), Query Frame = 0

Query: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYL 60
            ME GGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFY+DKVQ IPESFKSVHQYLGSYL
Sbjct: 1    MEVGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYRDKVQNIPESFKSVHQYLGSYL 60

Query: 61   FPLLEETRAELSLSLKAIRRAPFAQLVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYR 120
            FPLLEETRAELS SLKAI RAPFAQLVSIE PKSSG+L LNVN+D+W+NT+NN GKEPYR
Sbjct: 61   FPLLEETRAELSSSLKAIHRAPFAQLVSIEVPKSSGKLSLNVNIDAWKNTSNNSGKEPYR 120

Query: 121  TLPGDIFLILDDKPETFMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQK 180
            TLPGDIFLILDDKPET M  Q  TRTWAFAWV +IT+ GCS+HLKLNVSKNISGE GMQK
Sbjct: 121  TLPGDIFLILDDKPETGMNLQRPTRTWAFAWVKKITDTGCSTHLKLNVSKNISGEQGMQK 180

Query: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLSTKSMGDKICNKCSLFNNAVCAEKLGS 240
            EFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLS KSMGD+IC+KCSL+NN VCAEKLG+
Sbjct: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLSLKSMGDEICSKCSLYNNVVCAEKLGT 240

Query: 241  NLSSVLNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILA 300
            +LSSVLNDSQK+AVLCSVCKTLCDHKPSVELIWGPPGTGKTKTIS LL AILEMKQR++A
Sbjct: 241  SLSSVLNDSQKAAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISILLCAILEMKQRVVA 300

Query: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLKVGSELEEIYSDYRVD 360
            CAPTNVAITELA RVVKLLRESSR GGVLCSLGD+LLFGNKDRLKV  +LEEIY DYRVD
Sbjct: 301  CAPTNVAITELAFRVVKLLRESSRVGGVLCSLGDVLLFGNKDRLKVSFKLEEIYLDYRVD 360

Query: 361  RLVECFGQSGWKYHITSLIKLLENSNSEYHM-----VNMSRRGKTTGDNEVEVTSFLGFI 420
            RL+ECFGQSGWKYHITSLIKLLE+SNSEY M     VN SRR K  GDN VE TSFL FI
Sbjct: 361  RLLECFGQSGWKYHITSLIKLLESSNSEYSMFLESNVNASRRDKKKGDNVVEATSFLEFI 420

Query: 421  REKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEILLSQDNVTSVQME 480
            REKFK+TA+ALRGCL+TLITHIPKQFILEHNFQNIEILLNL+DS  +LLSQDNVTS+QME
Sbjct: 421  REKFKTTATALRGCLQTLITHIPKQFILEHNFQNIEILLNLVDSFGMLLSQDNVTSMQME 480

Query: 481  ILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPSTANKKSVRKFCFQ 540
            ILFSS EVF MDFPNSSVEATFL+LR+QC+SILR LQASLDQLQLPSTANKKSV+KFC Q
Sbjct: 481  ILFSSLEVF-MDFPNSSVEATFLHLRNQCVSILRFLQASLDQLQLPSTANKKSVKKFCLQ 540

Query: 541  RASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGIKHAILIGDECQL 600
            RASLILCTAS+SFQLNSMKMDPVN LVIDEAAQLKECESIV LQ+PGIKHAILIGDECQL
Sbjct: 541  RASLILCTASSSFQLNSMKMDPVNFLVIDEAAQLKECESIVALQLPGIKHAILIGDECQL 600

Query: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYNNQILDAPL 660
            PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFY+N+ILDAPL
Sbjct: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYSNKILDAPL 660

Query: 661  VMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLM 720
            VMDKVHKK +IPSPMFGPY FINVSVGKEEGDDD HSKKN VEVAVVIKIIEKLY     
Sbjct: 661  VMDKVHKKHYIPSPMFGPYTFINVSVGKEEGDDDVHSKKNMVEVAVVIKIIEKLY----- 720

Query: 721  QLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYE 780
                                      KA RSAKT LSIGVISFYAAQVSAIQGRLGQKYE
Sbjct: 721  --------------------------KAWRSAKTRLSIGVISFYAAQVSAIQGRLGQKYE 780

Query: 781  KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINVALTRARHCLWIVG 840
            KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNR KNIGFISNSQRINVALTRARHCLWIVG
Sbjct: 781  KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRRKNIGFISNSQRINVALTRARHCLWIVG 840

Query: 841  DATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSLLFK 900
            DATTLG+SNSEWEAVVS+AK RQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDS LFK
Sbjct: 841  DATTLGDSNSEWEAVVSDAKDRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSALFK 900

Query: 901  MAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACGWRPETDYVSNPKCSNIIKCVKV 960
            M QWKVLLSDSFRASFQ+VVSINQKKSIIV+LLRL+CGWRPET+ VSNPKCS+IIKCVKV
Sbjct: 901  MVQWKVLLSDSFRASFQEVVSINQKKSIIVLLLRLSCGWRPETN-VSNPKCSDIIKCVKV 960

Query: 961  EGLFIIYSLDIEKDSKYKQILKIWDIKPLTDVKVLVDCLSNIHELYTDDFLNLCKANSHK 1020
            EGLFIIYSLDIEKDSKYKQ+LKIWDIKPLTDVK LVDCLSNIHELYTDDFLNLCK  S K
Sbjct: 961  EGLFIIYSLDIEKDSKYKQVLKIWDIKPLTDVKGLVDCLSNIHELYTDDFLNLCKTKSDK 1020

Query: 1021 GDLELPITWSDSHDIVVYKDHMKAELNAILSLQADTDDTKNITLKKNLLQMKFQSLSYQK 1080
            GDLELPITWS SHDIVVYKDHMKAEL+AILSLQAD+DDTKNITLKKNLLQMKFQSLSYQK
Sbjct: 1021 GDLELPITWSASHDIVVYKDHMKAELDAILSLQADSDDTKNITLKKNLLQMKFQSLSYQK 1080

Query: 1081 AKHLLSSRDSKELDLPCQVEDEQLEVILFPTSAFIMGRPGPGKTAALTIKLFMREQQQQI 1140
            AKHLLSS DSKELDLPCQVEDEQLE+IL PTSAF+MGRP  GKTAALTIKLFMREQQQQI
Sbjct: 1081 AKHLLSSHDSKELDLPCQVEDEQLEIILCPTSAFLMGRPSYGKTAALTIKLFMREQQQQI 1140

Query: 1141 HPEVRCQVTRENAQVTYTMEGGEECKKIDRTVLRQLFITVTLKQCLAVKEHLSYLKR 1193
            HPE   +VTR+NA+V    EGGEECK+I RTVLRQLFITVTLKQCLAVKEHLSYLKR
Sbjct: 1141 HPEGCSEVTRQNAEVCCRNEGGEECKRIGRTVLRQLFITVTLKQCLAVKEHLSYLKR 1164

BLAST of Spg035353 vs. NCBI nr
Match: XP_031742058.1 (uncharacterized protein LOC101214394 isoform X2 [Cucumis sativus])

HSP 1 Score: 1978.8 bits (5125), Expect = 0.0e+00
Identity = 1012/1197 (84.54%), Postives = 1083/1197 (90.48%), Query Frame = 0

Query: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYL 60
            MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFY+DKVQ IPESFKSVHQYLGSYL
Sbjct: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYRDKVQNIPESFKSVHQYLGSYL 60

Query: 61   FPLLEETRAELSLSLKAIRRAPFAQLVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYR 120
            FPLLEETRAELS  LKAI +APFA++VSIEEPKSSG+LLLNV +D W+NTANN GKEPYR
Sbjct: 61   FPLLEETRAELSSGLKAIHKAPFARMVSIEEPKSSGKLLLNVKLDVWKNTANNSGKEPYR 120

Query: 121  TLPGDIFLILDDKPETFMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQK 180
            TLPGDIFLILDDKPET M  Q STRTWAFA VN+IT+ GCS++LKLNVSKNISGEHGMQK
Sbjct: 121  TLPGDIFLILDDKPETDMNLQCSTRTWAFASVNKITDTGCSTNLKLNVSKNISGEHGMQK 180

Query: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLSTKSMGDKICNKCSLFNNAVCAEKLGS 240
            EFFIVFLMNVTTNLRIWNSLHFSEDVKI+KHVLS  SMGD+IC+KCSL+NN +CAEKL +
Sbjct: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIVKHVLSKSSMGDEICSKCSLYNNVICAEKLRT 240

Query: 241  NLSSVLNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILA 300
            +LSSVLNDSQK+AVLC VCK LC+HKPSVELIWGPPGTGKTKTISFLLWAILEMKQR+LA
Sbjct: 241  SLSSVLNDSQKAAVLCCVCKALCEHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRVLA 300

Query: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLKVGSELEEIYSDYRVD 360
            CAPTNVAITELASRVVKLLRESSREGGVLCSLGD+LLFGNKDRLKVGSELEEIYSDYRVD
Sbjct: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDVLLFGNKDRLKVGSELEEIYSDYRVD 360

Query: 361  RLVECFGQSGWKYHITSLIKLLENSNSEYHM-----VNMSRRGKTTGDNEVEVTSFLGFI 420
            RL+ECFGQSGWK HITSLI LLE++NSEYHM     VNMSRR K TGDN V  TSFL FI
Sbjct: 361  RLLECFGQSGWKSHITSLINLLESTNSEYHMFLESNVNMSRRDKKTGDNAVAATSFLRFI 420

Query: 421  REKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEILLSQDNVTSVQME 480
            REKF +TA ALRGCL+TLITHIPK FILEHNFQNI ILLNL+DS  +LLSQ+N+TS QME
Sbjct: 421  REKFNTTAVALRGCLQTLITHIPKHFILEHNFQNIVILLNLVDSFGMLLSQENITSTQME 480

Query: 481  ILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPSTANKKSVRKFCFQ 540
            +LFSS +VF M+FPNSSVEATFL+LR+QCLSILR LQASLDQLQLP+TANKKSV++FCFQ
Sbjct: 481  VLFSSLDVF-MEFPNSSVEATFLHLRNQCLSILRFLQASLDQLQLPTTANKKSVKEFCFQ 540

Query: 541  RASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGIKHAILIGDECQL 600
            RASLILCTAS+SFQLN MKMDPV LLVIDEAAQLKECES+VPLQ+PGIKHAILIGDECQL
Sbjct: 541  RASLILCTASSSFQLNFMKMDPVKLLVIDEAAQLKECESMVPLQLPGIKHAILIGDECQL 600

Query: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYNNQILDAPL 660
            PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSIS FPNSKFY+NQI DAPL
Sbjct: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISYFPNSKFYSNQITDAPL 660

Query: 661  VMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLM 720
            VMD+V+KK +IPSPMFGPY FINVSVGKEEGDDDG SKKN +EVAVVIKIIEKLY     
Sbjct: 661  VMDEVYKKRYIPSPMFGPYTFINVSVGKEEGDDDGRSKKNALEVAVVIKIIEKLY----- 720

Query: 721  QLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYE 780
                                      KA RS KT LSIGVISFYAAQV+AIQGRLGQKYE
Sbjct: 721  --------------------------KAWRSVKTRLSIGVISFYAAQVTAIQGRLGQKYE 780

Query: 781  KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINVALTRARHCLWIVG 840
            K D FTVKVKSVDGFQGGEEDVIILSTVRSNR K IGFISNSQRINVALTRARHCLWIVG
Sbjct: 781  KRDGFTVKVKSVDGFQGGEEDVIILSTVRSNRRKKIGFISNSQRINVALTRARHCLWIVG 840

Query: 841  DATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSLLFK 900
            DATTLGNSNSEWEAVVS+AK RQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDS+LFK
Sbjct: 841  DATTLGNSNSEWEAVVSDAKDRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSVLFK 900

Query: 901  MAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACGWRPETDYVSNPKCSNIIKCVKV 960
            M QWKVLLSDSFRASFQKVVS+NQKKSIIV+LLRL+CGWRPET    NPKCS+IIKCVKV
Sbjct: 901  MVQWKVLLSDSFRASFQKVVSVNQKKSIIVLLLRLSCGWRPETKNFPNPKCSDIIKCVKV 960

Query: 961  EGLFIIYSLDIEKDSKYKQILKIWDIKPLTDVKVLVDCLSNIHELYTDDFLNLCKANSHK 1020
            EGL+IIYSLDIEK SKYKQ+LKIWDIKPLTDVK +VDCLSNIHELYTD+FLNLC A+SHK
Sbjct: 961  EGLYIIYSLDIEKGSKYKQVLKIWDIKPLTDVKGVVDCLSNIHELYTDEFLNLCMASSHK 1020

Query: 1021 GDLELPITWSDSHDIVVYKDHMKAELNAILSLQADTDDTKNITLKKNLLQMKFQSLSYQK 1080
            GDLELPITWS SHDIVVYKDH+KAEL+AILS Q D+DDTKN+TLKKNLLQMKFQSLSYQK
Sbjct: 1021 GDLELPITWSASHDIVVYKDHIKAELDAILS-QDDSDDTKNVTLKKNLLQMKFQSLSYQK 1080

Query: 1081 AKHLLSSRDSKELDLPCQVEDEQLEVILFPTSAFIMGRPGPGKTAALTIKLFMREQQQQI 1140
            AK LLSS DSKELDLPCQVEDEQL++ILFPTSAF+MGRPG  KTAALTIKLFMRE+QQ I
Sbjct: 1081 AKLLLSSHDSKELDLPCQVEDEQLDIILFPTSAFVMGRPGSEKTAALTIKLFMREKQQLI 1140

Query: 1141 HPEVRCQVTRENAQVTYTMEGGEECKKIDRTVLRQLFITVTLKQCLAVKEHLSYLKR 1193
            HP+   +V R+NA+V Y  EGGEECKKIDRTVLRQLFITVTLKQCLAVKEHL YL R
Sbjct: 1141 HPKGCNEVMRQNAEVCYINEGGEECKKIDRTVLRQLFITVTLKQCLAVKEHLLYLSR 1164

BLAST of Spg035353 vs. NCBI nr
Match: XP_031742056.1 (uncharacterized protein LOC101214394 isoform X1 [Cucumis sativus] >XP_031742057.1 uncharacterized protein LOC101214394 isoform X1 [Cucumis sativus] >KAE8648090.1 hypothetical protein Csa_004704 [Cucumis sativus])

HSP 1 Score: 1978.8 bits (5125), Expect = 0.0e+00
Identity = 1012/1197 (84.54%), Postives = 1083/1197 (90.48%), Query Frame = 0

Query: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYL 60
            MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFY+DKVQ IPESFKSVHQYLGSYL
Sbjct: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYRDKVQNIPESFKSVHQYLGSYL 60

Query: 61   FPLLEETRAELSLSLKAIRRAPFAQLVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYR 120
            FPLLEETRAELS  LKAI +APFA++VSIEEPKSSG+LLLNV +D W+NTANN GKEPYR
Sbjct: 61   FPLLEETRAELSSGLKAIHKAPFARMVSIEEPKSSGKLLLNVKLDVWKNTANNSGKEPYR 120

Query: 121  TLPGDIFLILDDKPETFMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQK 180
            TLPGDIFLILDDKPET M  Q STRTWAFA VN+IT+ GCS++LKLNVSKNISGEHGMQK
Sbjct: 121  TLPGDIFLILDDKPETDMNLQCSTRTWAFASVNKITDTGCSTNLKLNVSKNISGEHGMQK 180

Query: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLSTKSMGDKICNKCSLFNNAVCAEKLGS 240
            EFFIVFLMNVTTNLRIWNSLHFSEDVKI+KHVLS  SMGD+IC+KCSL+NN +CAEKL +
Sbjct: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIVKHVLSKSSMGDEICSKCSLYNNVICAEKLRT 240

Query: 241  NLSSVLNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILA 300
            +LSSVLNDSQK+AVLC VCK LC+HKPSVELIWGPPGTGKTKTISFLLWAILEMKQR+LA
Sbjct: 241  SLSSVLNDSQKAAVLCCVCKALCEHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRVLA 300

Query: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLKVGSELEEIYSDYRVD 360
            CAPTNVAITELASRVVKLLRESSREGGVLCSLGD+LLFGNKDRLKVGSELEEIYSDYRVD
Sbjct: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDVLLFGNKDRLKVGSELEEIYSDYRVD 360

Query: 361  RLVECFGQSGWKYHITSLIKLLENSNSEYHM-----VNMSRRGKTTGDNEVEVTSFLGFI 420
            RL+ECFGQSGWK HITSLI LLE++NSEYHM     VNMSRR K TGDN V  TSFL FI
Sbjct: 361  RLLECFGQSGWKSHITSLINLLESTNSEYHMFLESNVNMSRRDKKTGDNAVAATSFLRFI 420

Query: 421  REKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEILLSQDNVTSVQME 480
            REKF +TA ALRGCL+TLITHIPK FILEHNFQNI ILLNL+DS  +LLSQ+N+TS QME
Sbjct: 421  REKFNTTAVALRGCLQTLITHIPKHFILEHNFQNIVILLNLVDSFGMLLSQENITSTQME 480

Query: 481  ILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPSTANKKSVRKFCFQ 540
            +LFSS +VF M+FPNSSVEATFL+LR+QCLSILR LQASLDQLQLP+TANKKSV++FCFQ
Sbjct: 481  VLFSSLDVF-MEFPNSSVEATFLHLRNQCLSILRFLQASLDQLQLPTTANKKSVKEFCFQ 540

Query: 541  RASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGIKHAILIGDECQL 600
            RASLILCTAS+SFQLN MKMDPV LLVIDEAAQLKECES+VPLQ+PGIKHAILIGDECQL
Sbjct: 541  RASLILCTASSSFQLNFMKMDPVKLLVIDEAAQLKECESMVPLQLPGIKHAILIGDECQL 600

Query: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYNNQILDAPL 660
            PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSIS FPNSKFY+NQI DAPL
Sbjct: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISYFPNSKFYSNQITDAPL 660

Query: 661  VMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLM 720
            VMD+V+KK +IPSPMFGPY FINVSVGKEEGDDDG SKKN +EVAVVIKIIEKLY     
Sbjct: 661  VMDEVYKKRYIPSPMFGPYTFINVSVGKEEGDDDGRSKKNALEVAVVIKIIEKLY----- 720

Query: 721  QLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYE 780
                                      KA RS KT LSIGVISFYAAQV+AIQGRLGQKYE
Sbjct: 721  --------------------------KAWRSVKTRLSIGVISFYAAQVTAIQGRLGQKYE 780

Query: 781  KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINVALTRARHCLWIVG 840
            K D FTVKVKSVDGFQGGEEDVIILSTVRSNR K IGFISNSQRINVALTRARHCLWIVG
Sbjct: 781  KRDGFTVKVKSVDGFQGGEEDVIILSTVRSNRRKKIGFISNSQRINVALTRARHCLWIVG 840

Query: 841  DATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSLLFK 900
            DATTLGNSNSEWEAVVS+AK RQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDS+LFK
Sbjct: 841  DATTLGNSNSEWEAVVSDAKDRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSVLFK 900

Query: 901  MAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACGWRPETDYVSNPKCSNIIKCVKV 960
            M QWKVLLSDSFRASFQKVVS+NQKKSIIV+LLRL+CGWRPET    NPKCS+IIKCVKV
Sbjct: 901  MVQWKVLLSDSFRASFQKVVSVNQKKSIIVLLLRLSCGWRPETKNFPNPKCSDIIKCVKV 960

Query: 961  EGLFIIYSLDIEKDSKYKQILKIWDIKPLTDVKVLVDCLSNIHELYTDDFLNLCKANSHK 1020
            EGL+IIYSLDIEK SKYKQ+LKIWDIKPLTDVK +VDCLSNIHELYTD+FLNLC A+SHK
Sbjct: 961  EGLYIIYSLDIEKGSKYKQVLKIWDIKPLTDVKGVVDCLSNIHELYTDEFLNLCMASSHK 1020

Query: 1021 GDLELPITWSDSHDIVVYKDHMKAELNAILSLQADTDDTKNITLKKNLLQMKFQSLSYQK 1080
            GDLELPITWS SHDIVVYKDH+KAEL+AILS Q D+DDTKN+TLKKNLLQMKFQSLSYQK
Sbjct: 1021 GDLELPITWSASHDIVVYKDHIKAELDAILS-QDDSDDTKNVTLKKNLLQMKFQSLSYQK 1080

Query: 1081 AKHLLSSRDSKELDLPCQVEDEQLEVILFPTSAFIMGRPGPGKTAALTIKLFMREQQQQI 1140
            AK LLSS DSKELDLPCQVEDEQL++ILFPTSAF+MGRPG  KTAALTIKLFMRE+QQ I
Sbjct: 1081 AKLLLSSHDSKELDLPCQVEDEQLDIILFPTSAFVMGRPGSEKTAALTIKLFMREKQQLI 1140

Query: 1141 HPEVRCQVTRENAQVTYTMEGGEECKKIDRTVLRQLFITVTLKQCLAVKEHLSYLKR 1193
            HP+   +V R+NA+V Y  EGGEECKKIDRTVLRQLFITVTLKQCLAVKEHL YL R
Sbjct: 1141 HPKGCNEVMRQNAEVCYINEGGEECKKIDRTVLRQLFITVTLKQCLAVKEHLLYLSR 1164

BLAST of Spg035353 vs. NCBI nr
Match: TYK30457.1 (uncharacterized protein E5676_scaffold349G00820 [Cucumis melo var. makuwa])

HSP 1 Score: 1978.4 bits (5124), Expect = 0.0e+00
Identity = 1011/1197 (84.46%), Postives = 1083/1197 (90.48%), Query Frame = 0

Query: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYL 60
            MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFY+DKVQ IPESFKSVHQYLGSY 
Sbjct: 25   MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYRDKVQNIPESFKSVHQYLGSYH 84

Query: 61   FPLLEETRAELSLSLKAIRRAPFAQLVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYR 120
            FPLLEETRAELS SLKAI +APFA++V IEEPKSSG+LLLNV +D+W+NT NN GKE YR
Sbjct: 85   FPLLEETRAELSSSLKAIHKAPFARMVYIEEPKSSGKLLLNVKLDAWKNTTNNSGKESYR 144

Query: 121  TLPGDIFLILDDKPETFMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQK 180
            TLPGDIFLILDDKP T +  Q STRTWAFAWVN+IT+ GCS++LKLNVSKNISGEHGMQK
Sbjct: 145  TLPGDIFLILDDKPGTDINLQCSTRTWAFAWVNKITDTGCSTNLKLNVSKNISGEHGMQK 204

Query: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLSTKSMGDKICNKCSLFNNAVCAEKLGS 240
            EFF VFLMNVTTNLRIWNSLHFSEDVKI+KHVLS  SMGD+IC+KCS +NN +CAEKL +
Sbjct: 205  EFFSVFLMNVTTNLRIWNSLHFSEDVKIVKHVLSKNSMGDEICSKCSSYNNVICAEKLRT 264

Query: 241  NLSSVLNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILA 300
            +LSS LNDSQK+AVLC VCKTLC+HKPSVELIWGPPGTGKTKTISFLLWAILEMKQR+LA
Sbjct: 265  SLSSALNDSQKAAVLCCVCKTLCEHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRVLA 324

Query: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLKVGSELEEIYSDYRVD 360
            CAPTNVAITELASRVVKLLRESSREGGVLCSLGD+LLFGNKDRLKVGSELEEIYSDYRVD
Sbjct: 325  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDVLLFGNKDRLKVGSELEEIYSDYRVD 384

Query: 361  RLVECFGQSGWKYHITSLIKLLENSNSEYHM-----VNMSRRGKTTGDNEVEVTSFLGFI 420
            RL+ECFGQSGWK HITSLIKLLE+SNSEYHM      N+SRR K TGD+ V  TSFL FI
Sbjct: 385  RLLECFGQSGWKSHITSLIKLLESSNSEYHMFLESNANLSRRDKKTGDDVVAATSFLRFI 444

Query: 421  REKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEILLSQDNVTSVQME 480
            REKF +TA ALRGCL+TLITHIPKQFILEHNFQNI ILLNL+DS  +LLSQDN+TS QME
Sbjct: 445  REKFNTTAVALRGCLQTLITHIPKQFILEHNFQNIVILLNLVDSFGMLLSQDNITSTQME 504

Query: 481  ILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPSTANKKSVRKFCFQ 540
            +LFSS +V +MDFPNSSVEATFL+LR+QCLSILR LQASLDQLQLP+TANKKSV+KFCFQ
Sbjct: 505  VLFSSLDV-IMDFPNSSVEATFLHLRNQCLSILRFLQASLDQLQLPTTANKKSVKKFCFQ 564

Query: 541  RASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGIKHAILIGDECQL 600
            RASLILCTAS+SFQLNSMKMDPV LLVIDEAAQLKECES+VPLQ+PGIKHAILIGDECQL
Sbjct: 565  RASLILCTASSSFQLNSMKMDPVKLLVIDEAAQLKECESVVPLQLPGIKHAILIGDECQL 624

Query: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYNNQILDAPL 660
            PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSIS FP+SKFY+NQI DAPL
Sbjct: 625  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISYFPSSKFYSNQITDAPL 684

Query: 661  VMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLM 720
            VMD+ +KK +IPSPMFGPY FINVSVGKEEGDDDGHSKKN VEVAVVIKIIEKLY     
Sbjct: 685  VMDEAYKKRYIPSPMFGPYTFINVSVGKEEGDDDGHSKKNAVEVAVVIKIIEKLY----- 744

Query: 721  QLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYE 780
                                      +A RS KT LSIGVISFYAAQVSAIQGRLGQKYE
Sbjct: 745  --------------------------RAWRSVKTRLSIGVISFYAAQVSAIQGRLGQKYE 804

Query: 781  KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINVALTRARHCLWIVG 840
            KS  FTVKVKSVDGFQGGEEDVIILSTVRSNR KNIGFISNSQRINVALTRARHCLWIVG
Sbjct: 805  KSKGFTVKVKSVDGFQGGEEDVIILSTVRSNRRKNIGFISNSQRINVALTRARHCLWIVG 864

Query: 841  DATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSLLFK 900
            DATTLGNSNSEWEAVVS+AK RQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDS+LFK
Sbjct: 865  DATTLGNSNSEWEAVVSDAKDRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSVLFK 924

Query: 901  MAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACGWRPETDYVSNPKCSNIIKCVKV 960
            M QWKVLLSDSFRASFQKVVSINQKKSIIV+LLRL+CGWRPET   SNPKCS+II C KV
Sbjct: 925  MVQWKVLLSDSFRASFQKVVSINQKKSIIVLLLRLSCGWRPETKNFSNPKCSDIINCAKV 984

Query: 961  EGLFIIYSLDIEKDSKYKQILKIWDIKPLTDVKVLVDCLSNIHELYTDDFLNLCKANSHK 1020
            EGL+IIYSLDIEKDS+YKQ+LKIWDIKPLTDVK +VDCLSNIHELYTDDFLNLC ANSHK
Sbjct: 985  EGLYIIYSLDIEKDSEYKQVLKIWDIKPLTDVKGVVDCLSNIHELYTDDFLNLCMANSHK 1044

Query: 1021 GDLELPITWSDSHDIVVYKDHMKAELNAILSLQADTDDTKNITLKKNLLQMKFQSLSYQK 1080
            GDL+LPITWS SHDIVVYKDH+KA+L+AILS Q D+DDTKN TLKKNLLQMKFQSLSYQK
Sbjct: 1045 GDLKLPITWSASHDIVVYKDHIKADLDAILS-QDDSDDTKNATLKKNLLQMKFQSLSYQK 1104

Query: 1081 AKHLLSSRDSKELDLPCQVEDEQLEVILFPTSAFIMGRPGPGKTAALTIKLFMREQQQQI 1140
            AK LLSS DSKELDLPCQVEDEQL++ILFPTSAFIMGRPG GKTAALTIKLFMRE+QQ+I
Sbjct: 1105 AKLLLSSHDSKELDLPCQVEDEQLDIILFPTSAFIMGRPGLGKTAALTIKLFMREKQQEI 1164

Query: 1141 HPEVRCQVTRENAQVTYTMEGGEECKKIDRTVLRQLFITVTLKQCLAVKEHLSYLKR 1193
            HP+   +V R+NA+V+Y  E GEECKKIDRTVLRQLFITVTLKQCLAVKEHL YL R
Sbjct: 1165 HPKGCNKVMRQNAEVSYINESGEECKKIDRTVLRQLFITVTLKQCLAVKEHLLYLSR 1188

BLAST of Spg035353 vs. NCBI nr
Match: XP_016901636.1 (PREDICTED: uncharacterized protein LOC103495157 [Cucumis melo])

HSP 1 Score: 1978.4 bits (5124), Expect = 0.0e+00
Identity = 1011/1197 (84.46%), Postives = 1083/1197 (90.48%), Query Frame = 0

Query: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYL 60
            MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFY+DKVQ IPESFKSVHQYLGSY 
Sbjct: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYRDKVQNIPESFKSVHQYLGSYH 60

Query: 61   FPLLEETRAELSLSLKAIRRAPFAQLVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYR 120
            FPLLEETRAELS SLKAI +APFA++V IEEPKSSG+LLLNV +D+W+NT NN GKE YR
Sbjct: 61   FPLLEETRAELSSSLKAIHKAPFARMVYIEEPKSSGKLLLNVKLDAWKNTTNNSGKESYR 120

Query: 121  TLPGDIFLILDDKPETFMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQK 180
            TLPGDIFLILDDKP T +  Q STRTWAFAWVN+IT+ GCS++LKLNVSKNISGEHGMQK
Sbjct: 121  TLPGDIFLILDDKPGTDINLQCSTRTWAFAWVNKITDTGCSTNLKLNVSKNISGEHGMQK 180

Query: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLSTKSMGDKICNKCSLFNNAVCAEKLGS 240
            EFF VFLMNVTTNLRIWNSLHFSEDVKI+KHVLS  SMGD+IC+KCS +NN +CAEKL +
Sbjct: 181  EFFSVFLMNVTTNLRIWNSLHFSEDVKIVKHVLSKNSMGDEICSKCSSYNNVICAEKLRT 240

Query: 241  NLSSVLNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILA 300
            +LSS LNDSQK+AVLC VCKTLC+HKPSVELIWGPPGTGKTKTISFLLWAILEMKQR+LA
Sbjct: 241  SLSSALNDSQKAAVLCCVCKTLCEHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRVLA 300

Query: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLKVGSELEEIYSDYRVD 360
            CAPTNVAITELASRVVKLLRESSREGGVLCSLGD+LLFGNKDRLKVGSELEEIYSDYRVD
Sbjct: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDVLLFGNKDRLKVGSELEEIYSDYRVD 360

Query: 361  RLVECFGQSGWKYHITSLIKLLENSNSEYHM-----VNMSRRGKTTGDNEVEVTSFLGFI 420
            RL+ECFGQSGWK HITSLIKLLE+SNSEYHM      N+SRR K TGD+ V  TSFL FI
Sbjct: 361  RLLECFGQSGWKSHITSLIKLLESSNSEYHMFLESNANLSRRDKKTGDDVVAATSFLRFI 420

Query: 421  REKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEILLSQDNVTSVQME 480
            REKF +TA ALRGCL+TLITHIPKQFILEHNFQNI ILLNL+DS  +LLSQDN+TS QME
Sbjct: 421  REKFNTTAVALRGCLQTLITHIPKQFILEHNFQNIVILLNLVDSFGMLLSQDNITSTQME 480

Query: 481  ILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPSTANKKSVRKFCFQ 540
            +LFSS +V +MDFPNSSVEATFL+LR+QCLSILR LQASLDQLQLP+TANKKSV+KFCFQ
Sbjct: 481  VLFSSLDV-IMDFPNSSVEATFLHLRNQCLSILRFLQASLDQLQLPTTANKKSVKKFCFQ 540

Query: 541  RASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGIKHAILIGDECQL 600
            RASLILCTAS+SFQLNSMKMDPV LLVIDEAAQLKECES+VPLQ+PGIKHAILIGDECQL
Sbjct: 541  RASLILCTASSSFQLNSMKMDPVKLLVIDEAAQLKECESVVPLQLPGIKHAILIGDECQL 600

Query: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYNNQILDAPL 660
            PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSIS FP+SKFY+NQI DAPL
Sbjct: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISYFPSSKFYSNQITDAPL 660

Query: 661  VMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLM 720
            VMD+ +KK +IPSPMFGPY FINVSVGKEEGDDDGHSKKN VEVAVVIKIIEKLY     
Sbjct: 661  VMDEAYKKRYIPSPMFGPYTFINVSVGKEEGDDDGHSKKNAVEVAVVIKIIEKLY----- 720

Query: 721  QLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYE 780
                                      +A RS KT LSIGVISFYAAQVSAIQGRLGQKYE
Sbjct: 721  --------------------------RAWRSVKTRLSIGVISFYAAQVSAIQGRLGQKYE 780

Query: 781  KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINVALTRARHCLWIVG 840
            KS  FTVKVKSVDGFQGGEEDVIILSTVRSNR KNIGFISNSQRINVALTRARHCLWIVG
Sbjct: 781  KSKGFTVKVKSVDGFQGGEEDVIILSTVRSNRRKNIGFISNSQRINVALTRARHCLWIVG 840

Query: 841  DATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSLLFK 900
            DATTLGNSNSEWEAVVS+AK RQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDS+LFK
Sbjct: 841  DATTLGNSNSEWEAVVSDAKDRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSVLFK 900

Query: 901  MAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACGWRPETDYVSNPKCSNIIKCVKV 960
            M QWKVLLSDSFRASFQKVVSINQKKSIIV+LLRL+CGWRPET   SNPKCS+II C KV
Sbjct: 901  MVQWKVLLSDSFRASFQKVVSINQKKSIIVLLLRLSCGWRPETKNFSNPKCSDIINCAKV 960

Query: 961  EGLFIIYSLDIEKDSKYKQILKIWDIKPLTDVKVLVDCLSNIHELYTDDFLNLCKANSHK 1020
            EGL+IIYSLDIEKDS+YKQ+LKIWDIKPLTDVK +VDCLSNIHELYTDDFLNLC ANSHK
Sbjct: 961  EGLYIIYSLDIEKDSEYKQVLKIWDIKPLTDVKGVVDCLSNIHELYTDDFLNLCMANSHK 1020

Query: 1021 GDLELPITWSDSHDIVVYKDHMKAELNAILSLQADTDDTKNITLKKNLLQMKFQSLSYQK 1080
            GDL+LPITWS SHDIVVYKDH+KA+L+AILS Q D+DDTKN TLKKNLLQMKFQSLSYQK
Sbjct: 1021 GDLKLPITWSASHDIVVYKDHIKADLDAILS-QDDSDDTKNATLKKNLLQMKFQSLSYQK 1080

Query: 1081 AKHLLSSRDSKELDLPCQVEDEQLEVILFPTSAFIMGRPGPGKTAALTIKLFMREQQQQI 1140
            AK LLSS DSKELDLPCQVEDEQL++ILFPTSAFIMGRPG GKTAALTIKLFMRE+QQ+I
Sbjct: 1081 AKLLLSSHDSKELDLPCQVEDEQLDIILFPTSAFIMGRPGLGKTAALTIKLFMREKQQEI 1140

Query: 1141 HPEVRCQVTRENAQVTYTMEGGEECKKIDRTVLRQLFITVTLKQCLAVKEHLSYLKR 1193
            HP+   +V R+NA+V+Y  E GEECKKIDRTVLRQLFITVTLKQCLAVKEHL YL R
Sbjct: 1141 HPKGCNKVMRQNAEVSYINESGEECKKIDRTVLRQLFITVTLKQCLAVKEHLLYLSR 1164

BLAST of Spg035353 vs. ExPASy Swiss-Prot
Match: B6SFA4 (Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 3.2e-37
Identity = 111/340 (32.65%), Postives = 168/340 (49.41%), Query Frame = 0

Query: 528 SVRKFCFQRASLILCTASTSFQLNSMKMD-PVNLLVIDEAAQLKECESIVPLQVPGIKHA 587
           S+R    + A+++  T S S      K +   ++++IDEAAQ  E  +++PL     K  
Sbjct: 453 SIRTAILEEAAIVFATLSFSGSALLAKSNRGFDVVIIDEAAQAVEPATLIPL-ATRCKQV 512

Query: 588 ILIGDECQLPAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFY 647
            L+GD  QLPA V S V   +GYG S+FERL   G+   +L TQYRMHP I  FP+ +FY
Sbjct: 513 FLVGDPKQLPATVISTVAQDSGYGTSMFERLQKAGYPVKMLKTQYRMHPEIRSFPSKQFY 572

Query: 648 NNQILDAPLVMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDG-HSKKNTVEVAVVIKI 707
              + D   + +    + +     FGP+ F ++  GKE        S+ N  EV  V+ I
Sbjct: 573 EGALEDGSDI-EAQTTRDWHKYRCFGPFCFFDIHEGKESQHPGATGSRVNLDEVEFVLLI 632

Query: 708 IEKLYKGMLMQLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSA 767
             +L   M  +L                              K+S  + +IS Y  QV  
Sbjct: 633 YHRLVT-MYPEL------------------------------KSSSQLAIISPYNYQVKT 692

Query: 768 IQGRLGQKYEKSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINVALT 827
            + R  + +    +  V + +VDGFQG E+DV I S VR+N    IGF+SNS+R+NV +T
Sbjct: 693 FKDRFKEMFGTEAEKVVDINTVDGFQGREKDVAIFSCVRANENGQIGFLSNSRRMNVGIT 752

Query: 828 RARHCLWIVGDATTLGNSNSEWEAVVSNAKYRQCYFNAEE 866
           RA+  + +VG A TL  S+  W+ ++ +A+ R   F   +
Sbjct: 753 RAKSSVLVVGSAATL-KSDPLWKNLIESAEQRNRLFKVSK 758

BLAST of Spg035353 vs. ExPASy Swiss-Prot
Match: O94387 (Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC29A10.10c PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 4.1e-37
Identity = 185/715 (25.87%), Postives = 297/715 (41.54%), Query Frame = 0

Query: 164  LKLNVSKNISGEHGMQKEFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLSTKSMGDKIC 223
            L++N+      E+     F    L N TT+LR + +L      K ++H+  ++ + D   
Sbjct: 1197 LRMNIESIDLQEYAPNIRFTAQKLFNATTSLREFAAL------KSLRHLPLSQRILD--A 1256

Query: 224  NKCSLFNNAVCAEKLGSNLSSVLNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKT 283
            N   L +N    +K     S  +N+ Q  A+  S             LI GPPGTGKTKT
Sbjct: 1257 NVTRLPSNFTDDKKQKIMKSYGVNEPQAYAIYAS------SVNDGFTLIQGPPGTGKTKT 1316

Query: 284  ISFLLWAIL-----------------EMKQRILACAPTNVAITELASRVVKLLRESSREG 343
            I  ++ A+L                   K +IL CAP+N AI E+  R+         + 
Sbjct: 1317 ILGMIGAVLTSSSQGLQFNVPGQTRKTSKNKILICAPSNAAIDEILLRI---------KA 1376

Query: 344  GVLCSLGDMLLFGNKDRLKVGSELEEIYSDYRVDRLVECFGQSGWKYHITSLIKLLENSN 403
            GV    G +  F    R+  G  +     ++ ++                 +IK +E +N
Sbjct: 1377 GVYDHEG-IKFFPKVIRVGFGDSISVHAKEFTLEE---------------QMIKQMELTN 1436

Query: 404  -SEYHMVNMSRRGKTTGDNEVEVTSFLGFIREKFKSTASALRGCLRTLITHIPKQFILEH 463
              +    N S   +   D+ ++    L    EKF+ST                   ILE 
Sbjct: 1437 LKKDQEANNSSDTRKKYDSIIKKRDSLREDLEKFRSTGK--------------NSSILEA 1496

Query: 464  NFQNIEILLNLID-SLEILLSQDNVTSVQMEILFSSPEVFMMDFPNSSVEATFLYLRSQC 523
              + I    N+++ SL+ +  +   T+  +++L                           
Sbjct: 1497 QLREITKQKNMLEQSLDDMRERQRSTNRNLDVL--------------------------- 1556

Query: 524  LSILRILQASLDQLQLPSTANKKSVRKFCFQRASLILCTASTS-FQLNSMKMDPVNLLVI 583
                                 KK ++    Q A ++  T S S  +L          ++I
Sbjct: 1557 ---------------------KKQIQNQLLQEADIVCATLSASGHELLLNAGLTFRTVII 1616

Query: 584  DEAAQLKECESIVPLQVPGIKHAILIGDECQLPAIVSSQVCDAAGYGRSLFERL-SLLGH 643
            DEAAQ  E  SI+PL+  G +  +++GD  QLP  V S+     GY +SL+ R+      
Sbjct: 1617 DEAAQAVELSSIIPLKY-GCESCVMVGDPNQLPPTVLSKTSAKFGYSQSLYVRMFKQHNE 1676

Query: 644  SKHLLNTQYRMHPSISCFPNSKFYNNQILDAPLVMDKVHKKSFIPSPMFGPYVFINVSVG 703
            S  LL+ QYRM+P IS FP+  FYN+++LD P  M  V  + +   P  G Y F NV   
Sbjct: 1677 SACLLSIQYRMNPEISRFPSKFFYNSKLLDGP-NMSAVTSRPWHEDPQLGIYRFFNVH-- 1736

Query: 704  KEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLMQLGHKWYFREEHGISFHQGAKPLSLNK 763
              E   +  S  N  E + ++ + E+L +  L              I F           
Sbjct: 1737 GTEAFSNSKSLYNVEEASFILLLYERLIQCYL-------------NIDFEG--------- 1774

Query: 764  ARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYEKSDKFTVKVKSVDGFQGGEEDVIILST 823
                      IGV++ Y +QV  ++ +  +KY       + + +VDGFQG E+D+II S 
Sbjct: 1797 ---------KIGVVTPYRSQVQQLRSQFQRKYGSIIFKHLDIHTVDGFQGQEKDIIIFSC 1774

Query: 824  VRSNRGKNIGFISNSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSNAKYR 858
            VRS+    IGF+ + +R+NVALTRA+  L+IVG++  L   +  + +++ +AK R
Sbjct: 1857 VRSSMSGGIGFLQDLRRLNVALTRAKSSLYIVGNSKPLMQEDI-FYSLIEDAKTR 1774

BLAST of Spg035353 vs. ExPASy Swiss-Prot
Match: Q9FJR0 (Regulator of nonsense transcripts 1 homolog OS=Arabidopsis thaliana OX=3702 GN=UPF1 PE=1 SV=2)

HSP 1 Score: 149.4 bits (376), Expect = 2.5e-34
Identity = 109/342 (31.87%), Postives = 167/342 (48.83%), Query Frame = 0

Query: 526 KKSVRKFCFQRASLILCT--ASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGI 585
           K++  +   Q A +I CT   +   +L++ +   V   +IDE+ Q  E E ++PL V G+
Sbjct: 619 KRATEREITQSADVICCTCVGAADLRLSNFRFRQV---LIDESTQATEPECLIPL-VLGV 678

Query: 586 KHAILIGDECQLPAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNS 645
           K  +L+GD CQL  ++  +    AG  +SLFERL  LG     L  QYRMHP++S FP++
Sbjct: 679 KQVVLVGDHCQLGPVIMCKKAARAGLAQSLFERLVTLGIKPIRLQVQYRMHPALSEFPSN 738

Query: 646 KFYNNQILDAPLVMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVI 705
            FY   + +   ++++       P P+    +F  V +G+EE    G S  N  E A V 
Sbjct: 739 SFYEGTLQNGVTIIERQTTGIDFPWPVPNRPMFFYVQLGQEEISASGTSYLNRTEAANVE 798

Query: 706 KIIEKLYKGMLMQLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQV 765
           K++    K                                  S      IGVI+ Y  Q 
Sbjct: 799 KLVTAFLK----------------------------------SGVVPSQIGVITPYEGQR 858

Query: 766 SAI------QGRLGQKYEKSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNS 825
           + I       G L Q+  K     ++V SVD FQG E+D IILS VRSN  + IGF+++ 
Sbjct: 859 AYIVNYMARNGSLRQQLYKE----IEVASVDSFQGREKDYIILSCVRSNEHQGIGFLNDP 917

Query: 826 QRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSNAKYRQC 860
           +R+NVALTRAR+ + I+G+   L +    W  ++++ K  +C
Sbjct: 919 RRLNVALTRARYGIVILGNPKVL-SKQPLWNGLLTHYKEHEC 917

BLAST of Spg035353 vs. ExPASy Swiss-Prot
Match: Q00416 (Helicase SEN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEN1 PE=1 SV=2)

HSP 1 Score: 149.4 bits (376), Expect = 2.5e-34
Identity = 113/384 (29.43%), Postives = 195/384 (50.78%), Query Frame = 0

Query: 489  PNSSVEATFLYLRSQCLS-ILRILQASLDQLQLPSTAN-------KKSVRKFCFQRASLI 548
            P S+ + + L L+ + LS I+  L    D+++  ++ N       +++ +      + +I
Sbjct: 1506 PMSTEDISKLQLKIRELSKIINELGRDRDEMREKNSVNYRNRDLDRRNAQAHILAVSDII 1565

Query: 549  LCTASTS----FQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGIKHAILIGDECQLP 608
              T S S         +K D V   +IDEA Q  E  SI+PL+  G K  I++GD  QLP
Sbjct: 1566 CSTLSGSAHDVLATMGIKFDTV---IIDEACQCTELSSIIPLRYGG-KRCIMVGDPNQLP 1625

Query: 609  AIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYNNQILDAPLV 668
              V S       Y +SLF R+     S +LL+ QYRMHPSIS FP+S+FY  ++ D P  
Sbjct: 1626 PTVLSGAASNFKYNQSLFVRME-KNSSPYLLDVQYRMHPSISKFPSSEFYQGRLKDGP-G 1685

Query: 669  MDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLMQ 728
            MD ++K+ +       PY F ++  G++E +    S  N  E+ V I++++ L++     
Sbjct: 1686 MDILNKRPWHQLEPLAPYKFFDIISGRQEQNAKTMSYTNMEEIRVAIELVDYLFR----- 1745

Query: 729  LGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYEK 788
                   + ++ I F                  +  IG+IS Y  Q+  ++    + +  
Sbjct: 1746 -------KFDNKIDF------------------TGKIGIISPYREQMQKMRKEFARYFGG 1805

Query: 789  SDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGK-NIGFISNSQRINVALTRARHCLWIVG 848
                ++   ++DGFQG E+++I++S VR++  K ++GF+ + +R+NVALTRA+  +W++G
Sbjct: 1806 MINKSIDFNTIDGFQGQEKEIILISCVRADDTKSSVGFLKDFRRMNVALTRAKTSIWVLG 1852

Query: 849  DATTLGNSNSEWEAVVSNAKYRQC 860
               +L  S   W  ++ +AK R C
Sbjct: 1866 HQRSLAKSKL-WRDLIEDAKDRSC 1852

BLAST of Spg035353 vs. ExPASy Swiss-Prot
Match: Q9EPU0 (Regulator of nonsense transcripts 1 OS=Mus musculus OX=10090 GN=Upf1 PE=1 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 5.6e-34
Identity = 117/338 (34.62%), Postives = 162/338 (47.93%), Query Frame = 0

Query: 524 ANKKSVRKFCFQRASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGI 583
           A K++  +     A +I CT   +      KM     ++IDE+ Q  E E +VP+ V G 
Sbjct: 603 ALKRTAERELLMNADVICCTCVGAGDPRLAKMQ-FRSILIDESTQATEPECMVPV-VLGA 662

Query: 584 KHAILIGDECQLPAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNS 643
           K  IL+GD CQL  +V  +    AG  +SLFERL +LG     L  QYRMHP++S FP++
Sbjct: 663 KQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSN 722

Query: 644 KFYNNQILDAPLVMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVI 703
            FY   + +     D+V K      P     +F  V+ G+EE    G S  N  E A V 
Sbjct: 723 IFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVE 782

Query: 704 KIIEKLYKGMLMQLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQV 763
           KI  KL K                      GAKP               IG+I+ Y  Q 
Sbjct: 783 KITTKLLKA---------------------GAKP-------------DQIGIITPYEGQR 842

Query: 764 SAI------QGRLGQKYEKSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNS 823
           S +       G L  K  +     V++ SVD FQG E+D IILS VR+N  + IGF+++ 
Sbjct: 843 SYLVQYMQFSGSLHTKLYQE----VEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDP 899

Query: 824 QRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSNAK 856
           +R+NVALTRAR+ + IVG+   L +    W  ++S  K
Sbjct: 903 RRLNVALTRARYGVIIVGNPKAL-SKQPLWNHLLSYYK 899

BLAST of Spg035353 vs. ExPASy TrEMBL
Match: A0A5D3E306 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold349G00820 PE=4 SV=1)

HSP 1 Score: 1978.4 bits (5124), Expect = 0.0e+00
Identity = 1011/1197 (84.46%), Postives = 1083/1197 (90.48%), Query Frame = 0

Query: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYL 60
            MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFY+DKVQ IPESFKSVHQYLGSY 
Sbjct: 25   MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYRDKVQNIPESFKSVHQYLGSYH 84

Query: 61   FPLLEETRAELSLSLKAIRRAPFAQLVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYR 120
            FPLLEETRAELS SLKAI +APFA++V IEEPKSSG+LLLNV +D+W+NT NN GKE YR
Sbjct: 85   FPLLEETRAELSSSLKAIHKAPFARMVYIEEPKSSGKLLLNVKLDAWKNTTNNSGKESYR 144

Query: 121  TLPGDIFLILDDKPETFMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQK 180
            TLPGDIFLILDDKP T +  Q STRTWAFAWVN+IT+ GCS++LKLNVSKNISGEHGMQK
Sbjct: 145  TLPGDIFLILDDKPGTDINLQCSTRTWAFAWVNKITDTGCSTNLKLNVSKNISGEHGMQK 204

Query: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLSTKSMGDKICNKCSLFNNAVCAEKLGS 240
            EFF VFLMNVTTNLRIWNSLHFSEDVKI+KHVLS  SMGD+IC+KCS +NN +CAEKL +
Sbjct: 205  EFFSVFLMNVTTNLRIWNSLHFSEDVKIVKHVLSKNSMGDEICSKCSSYNNVICAEKLRT 264

Query: 241  NLSSVLNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILA 300
            +LSS LNDSQK+AVLC VCKTLC+HKPSVELIWGPPGTGKTKTISFLLWAILEMKQR+LA
Sbjct: 265  SLSSALNDSQKAAVLCCVCKTLCEHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRVLA 324

Query: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLKVGSELEEIYSDYRVD 360
            CAPTNVAITELASRVVKLLRESSREGGVLCSLGD+LLFGNKDRLKVGSELEEIYSDYRVD
Sbjct: 325  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDVLLFGNKDRLKVGSELEEIYSDYRVD 384

Query: 361  RLVECFGQSGWKYHITSLIKLLENSNSEYHM-----VNMSRRGKTTGDNEVEVTSFLGFI 420
            RL+ECFGQSGWK HITSLIKLLE+SNSEYHM      N+SRR K TGD+ V  TSFL FI
Sbjct: 385  RLLECFGQSGWKSHITSLIKLLESSNSEYHMFLESNANLSRRDKKTGDDVVAATSFLRFI 444

Query: 421  REKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEILLSQDNVTSVQME 480
            REKF +TA ALRGCL+TLITHIPKQFILEHNFQNI ILLNL+DS  +LLSQDN+TS QME
Sbjct: 445  REKFNTTAVALRGCLQTLITHIPKQFILEHNFQNIVILLNLVDSFGMLLSQDNITSTQME 504

Query: 481  ILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPSTANKKSVRKFCFQ 540
            +LFSS +V +MDFPNSSVEATFL+LR+QCLSILR LQASLDQLQLP+TANKKSV+KFCFQ
Sbjct: 505  VLFSSLDV-IMDFPNSSVEATFLHLRNQCLSILRFLQASLDQLQLPTTANKKSVKKFCFQ 564

Query: 541  RASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGIKHAILIGDECQL 600
            RASLILCTAS+SFQLNSMKMDPV LLVIDEAAQLKECES+VPLQ+PGIKHAILIGDECQL
Sbjct: 565  RASLILCTASSSFQLNSMKMDPVKLLVIDEAAQLKECESVVPLQLPGIKHAILIGDECQL 624

Query: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYNNQILDAPL 660
            PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSIS FP+SKFY+NQI DAPL
Sbjct: 625  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISYFPSSKFYSNQITDAPL 684

Query: 661  VMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLM 720
            VMD+ +KK +IPSPMFGPY FINVSVGKEEGDDDGHSKKN VEVAVVIKIIEKLY     
Sbjct: 685  VMDEAYKKRYIPSPMFGPYTFINVSVGKEEGDDDGHSKKNAVEVAVVIKIIEKLY----- 744

Query: 721  QLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYE 780
                                      +A RS KT LSIGVISFYAAQVSAIQGRLGQKYE
Sbjct: 745  --------------------------RAWRSVKTRLSIGVISFYAAQVSAIQGRLGQKYE 804

Query: 781  KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINVALTRARHCLWIVG 840
            KS  FTVKVKSVDGFQGGEEDVIILSTVRSNR KNIGFISNSQRINVALTRARHCLWIVG
Sbjct: 805  KSKGFTVKVKSVDGFQGGEEDVIILSTVRSNRRKNIGFISNSQRINVALTRARHCLWIVG 864

Query: 841  DATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSLLFK 900
            DATTLGNSNSEWEAVVS+AK RQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDS+LFK
Sbjct: 865  DATTLGNSNSEWEAVVSDAKDRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSVLFK 924

Query: 901  MAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACGWRPETDYVSNPKCSNIIKCVKV 960
            M QWKVLLSDSFRASFQKVVSINQKKSIIV+LLRL+CGWRPET   SNPKCS+II C KV
Sbjct: 925  MVQWKVLLSDSFRASFQKVVSINQKKSIIVLLLRLSCGWRPETKNFSNPKCSDIINCAKV 984

Query: 961  EGLFIIYSLDIEKDSKYKQILKIWDIKPLTDVKVLVDCLSNIHELYTDDFLNLCKANSHK 1020
            EGL+IIYSLDIEKDS+YKQ+LKIWDIKPLTDVK +VDCLSNIHELYTDDFLNLC ANSHK
Sbjct: 985  EGLYIIYSLDIEKDSEYKQVLKIWDIKPLTDVKGVVDCLSNIHELYTDDFLNLCMANSHK 1044

Query: 1021 GDLELPITWSDSHDIVVYKDHMKAELNAILSLQADTDDTKNITLKKNLLQMKFQSLSYQK 1080
            GDL+LPITWS SHDIVVYKDH+KA+L+AILS Q D+DDTKN TLKKNLLQMKFQSLSYQK
Sbjct: 1045 GDLKLPITWSASHDIVVYKDHIKADLDAILS-QDDSDDTKNATLKKNLLQMKFQSLSYQK 1104

Query: 1081 AKHLLSSRDSKELDLPCQVEDEQLEVILFPTSAFIMGRPGPGKTAALTIKLFMREQQQQI 1140
            AK LLSS DSKELDLPCQVEDEQL++ILFPTSAFIMGRPG GKTAALTIKLFMRE+QQ+I
Sbjct: 1105 AKLLLSSHDSKELDLPCQVEDEQLDIILFPTSAFIMGRPGLGKTAALTIKLFMREKQQEI 1164

Query: 1141 HPEVRCQVTRENAQVTYTMEGGEECKKIDRTVLRQLFITVTLKQCLAVKEHLSYLKR 1193
            HP+   +V R+NA+V+Y  E GEECKKIDRTVLRQLFITVTLKQCLAVKEHL YL R
Sbjct: 1165 HPKGCNKVMRQNAEVSYINESGEECKKIDRTVLRQLFITVTLKQCLAVKEHLLYLSR 1188

BLAST of Spg035353 vs. ExPASy TrEMBL
Match: A0A5A7T398 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold56G001350 PE=4 SV=1)

HSP 1 Score: 1978.4 bits (5124), Expect = 0.0e+00
Identity = 1011/1197 (84.46%), Postives = 1083/1197 (90.48%), Query Frame = 0

Query: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYL 60
            MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFY+DKVQ IPESFKSVHQYLGSY 
Sbjct: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYRDKVQNIPESFKSVHQYLGSYH 60

Query: 61   FPLLEETRAELSLSLKAIRRAPFAQLVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYR 120
            FPLLEETRAELS SLKAI +APFA++V IEEPKSSG+LLLNV +D+W+NT NN GKE YR
Sbjct: 61   FPLLEETRAELSSSLKAIHKAPFARMVYIEEPKSSGKLLLNVKLDAWKNTTNNSGKESYR 120

Query: 121  TLPGDIFLILDDKPETFMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQK 180
            TLPGDIFLILDDKP T +  Q STRTWAFAWVN+IT+ GCS++LKLNVSKNISGEHGMQK
Sbjct: 121  TLPGDIFLILDDKPGTDINLQCSTRTWAFAWVNKITDTGCSTNLKLNVSKNISGEHGMQK 180

Query: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLSTKSMGDKICNKCSLFNNAVCAEKLGS 240
            EFF VFLMNVTTNLRIWNSLHFSEDVKI+KHVLS  SMGD+IC+KCS +NN +CAEKL +
Sbjct: 181  EFFSVFLMNVTTNLRIWNSLHFSEDVKIVKHVLSKNSMGDEICSKCSSYNNVICAEKLRT 240

Query: 241  NLSSVLNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILA 300
            +LSS LNDSQK+AVLC VCKTLC+HKPSVELIWGPPGTGKTKTISFLLWAILEMKQR+LA
Sbjct: 241  SLSSALNDSQKAAVLCCVCKTLCEHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRVLA 300

Query: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLKVGSELEEIYSDYRVD 360
            CAPTNVAITELASRVVKLLRESSREGGVLCSLGD+LLFGNKDRLKVGSELEEIYSDYRVD
Sbjct: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDVLLFGNKDRLKVGSELEEIYSDYRVD 360

Query: 361  RLVECFGQSGWKYHITSLIKLLENSNSEYHM-----VNMSRRGKTTGDNEVEVTSFLGFI 420
            RL+ECFGQSGWK HITSLIKLLE+SNSEYHM      N+SRR K TGD+ V  TSFL FI
Sbjct: 361  RLLECFGQSGWKSHITSLIKLLESSNSEYHMFLESNANLSRRDKKTGDDVVAATSFLRFI 420

Query: 421  REKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEILLSQDNVTSVQME 480
            REKF +TA ALRGCL+TLITHIPKQFILEHNFQNI ILLNL+DS  +LLSQDN+TS QME
Sbjct: 421  REKFNTTAVALRGCLQTLITHIPKQFILEHNFQNIVILLNLVDSFGMLLSQDNITSTQME 480

Query: 481  ILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPSTANKKSVRKFCFQ 540
            +LFSS +V +MDFPNSSVEATFL+LR+QCLSILR LQASLDQLQLP+TANKKSV+KFCFQ
Sbjct: 481  VLFSSLDV-IMDFPNSSVEATFLHLRNQCLSILRFLQASLDQLQLPTTANKKSVKKFCFQ 540

Query: 541  RASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGIKHAILIGDECQL 600
            RASLILCTAS+SFQLNSMKMDPV LLVIDEAAQLKECES+VPLQ+PGIKHAILIGDECQL
Sbjct: 541  RASLILCTASSSFQLNSMKMDPVKLLVIDEAAQLKECESVVPLQLPGIKHAILIGDECQL 600

Query: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYNNQILDAPL 660
            PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSIS FP+SKFY+NQI DAPL
Sbjct: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISYFPSSKFYSNQITDAPL 660

Query: 661  VMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLM 720
            VMD+ +KK +IPSPMFGPY FINVSVGKEEGDDDGHSKKN VEVAVVIKIIEKLY     
Sbjct: 661  VMDEAYKKRYIPSPMFGPYTFINVSVGKEEGDDDGHSKKNAVEVAVVIKIIEKLY----- 720

Query: 721  QLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYE 780
                                      +A RS KT LSIGVISFYAAQVSAIQGRLGQKYE
Sbjct: 721  --------------------------RAWRSVKTRLSIGVISFYAAQVSAIQGRLGQKYE 780

Query: 781  KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINVALTRARHCLWIVG 840
            KS  FTVKVKSVDGFQGGEEDVIILSTVRSNR KNIGFISNSQRINVALTRARHCLWIVG
Sbjct: 781  KSKGFTVKVKSVDGFQGGEEDVIILSTVRSNRRKNIGFISNSQRINVALTRARHCLWIVG 840

Query: 841  DATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSLLFK 900
            DATTLGNSNSEWEAVVS+AK RQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDS+LFK
Sbjct: 841  DATTLGNSNSEWEAVVSDAKDRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSVLFK 900

Query: 901  MAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACGWRPETDYVSNPKCSNIIKCVKV 960
            M QWKVLLSDSFRASFQKVVSINQKKSIIV+LLRL+CGWRPET   SNPKCS+II C KV
Sbjct: 901  MVQWKVLLSDSFRASFQKVVSINQKKSIIVLLLRLSCGWRPETKNFSNPKCSDIINCAKV 960

Query: 961  EGLFIIYSLDIEKDSKYKQILKIWDIKPLTDVKVLVDCLSNIHELYTDDFLNLCKANSHK 1020
            EGL+IIYSLDIEKDS+YKQ+LKIWDIKPLTDVK +VDCLSNIHELYTDDFLNLC ANSHK
Sbjct: 961  EGLYIIYSLDIEKDSEYKQVLKIWDIKPLTDVKGVVDCLSNIHELYTDDFLNLCMANSHK 1020

Query: 1021 GDLELPITWSDSHDIVVYKDHMKAELNAILSLQADTDDTKNITLKKNLLQMKFQSLSYQK 1080
            GDL+LPITWS SHDIVVYKDH+KA+L+AILS Q D+DDTKN TLKKNLLQMKFQSLSYQK
Sbjct: 1021 GDLKLPITWSASHDIVVYKDHIKADLDAILS-QDDSDDTKNATLKKNLLQMKFQSLSYQK 1080

Query: 1081 AKHLLSSRDSKELDLPCQVEDEQLEVILFPTSAFIMGRPGPGKTAALTIKLFMREQQQQI 1140
            AK LLSS DSKELDLPCQVEDEQL++ILFPTSAFIMGRPG GKTAALTIKLFMRE+QQ+I
Sbjct: 1081 AKLLLSSHDSKELDLPCQVEDEQLDIILFPTSAFIMGRPGLGKTAALTIKLFMREKQQEI 1140

Query: 1141 HPEVRCQVTRENAQVTYTMEGGEECKKIDRTVLRQLFITVTLKQCLAVKEHLSYLKR 1193
            HP+   +V R+NA+V+Y  E GEECKKIDRTVLRQLFITVTLKQCLAVKEHL YL R
Sbjct: 1141 HPKGCNKVMRQNAEVSYINESGEECKKIDRTVLRQLFITVTLKQCLAVKEHLLYLSR 1164

BLAST of Spg035353 vs. ExPASy TrEMBL
Match: A0A1S4E082 (uncharacterized protein LOC103495157 OS=Cucumis melo OX=3656 GN=LOC103495157 PE=4 SV=1)

HSP 1 Score: 1978.4 bits (5124), Expect = 0.0e+00
Identity = 1011/1197 (84.46%), Postives = 1083/1197 (90.48%), Query Frame = 0

Query: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYL 60
            MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFY+DKVQ IPESFKSVHQYLGSY 
Sbjct: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYRDKVQNIPESFKSVHQYLGSYH 60

Query: 61   FPLLEETRAELSLSLKAIRRAPFAQLVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYR 120
            FPLLEETRAELS SLKAI +APFA++V IEEPKSSG+LLLNV +D+W+NT NN GKE YR
Sbjct: 61   FPLLEETRAELSSSLKAIHKAPFARMVYIEEPKSSGKLLLNVKLDAWKNTTNNSGKESYR 120

Query: 121  TLPGDIFLILDDKPETFMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQK 180
            TLPGDIFLILDDKP T +  Q STRTWAFAWVN+IT+ GCS++LKLNVSKNISGEHGMQK
Sbjct: 121  TLPGDIFLILDDKPGTDINLQCSTRTWAFAWVNKITDTGCSTNLKLNVSKNISGEHGMQK 180

Query: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLSTKSMGDKICNKCSLFNNAVCAEKLGS 240
            EFF VFLMNVTTNLRIWNSLHFSEDVKI+KHVLS  SMGD+IC+KCS +NN +CAEKL +
Sbjct: 181  EFFSVFLMNVTTNLRIWNSLHFSEDVKIVKHVLSKNSMGDEICSKCSSYNNVICAEKLRT 240

Query: 241  NLSSVLNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILA 300
            +LSS LNDSQK+AVLC VCKTLC+HKPSVELIWGPPGTGKTKTISFLLWAILEMKQR+LA
Sbjct: 241  SLSSALNDSQKAAVLCCVCKTLCEHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRVLA 300

Query: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLKVGSELEEIYSDYRVD 360
            CAPTNVAITELASRVVKLLRESSREGGVLCSLGD+LLFGNKDRLKVGSELEEIYSDYRVD
Sbjct: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDVLLFGNKDRLKVGSELEEIYSDYRVD 360

Query: 361  RLVECFGQSGWKYHITSLIKLLENSNSEYHM-----VNMSRRGKTTGDNEVEVTSFLGFI 420
            RL+ECFGQSGWK HITSLIKLLE+SNSEYHM      N+SRR K TGD+ V  TSFL FI
Sbjct: 361  RLLECFGQSGWKSHITSLIKLLESSNSEYHMFLESNANLSRRDKKTGDDVVAATSFLRFI 420

Query: 421  REKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEILLSQDNVTSVQME 480
            REKF +TA ALRGCL+TLITHIPKQFILEHNFQNI ILLNL+DS  +LLSQDN+TS QME
Sbjct: 421  REKFNTTAVALRGCLQTLITHIPKQFILEHNFQNIVILLNLVDSFGMLLSQDNITSTQME 480

Query: 481  ILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPSTANKKSVRKFCFQ 540
            +LFSS +V +MDFPNSSVEATFL+LR+QCLSILR LQASLDQLQLP+TANKKSV+KFCFQ
Sbjct: 481  VLFSSLDV-IMDFPNSSVEATFLHLRNQCLSILRFLQASLDQLQLPTTANKKSVKKFCFQ 540

Query: 541  RASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGIKHAILIGDECQL 600
            RASLILCTAS+SFQLNSMKMDPV LLVIDEAAQLKECES+VPLQ+PGIKHAILIGDECQL
Sbjct: 541  RASLILCTASSSFQLNSMKMDPVKLLVIDEAAQLKECESVVPLQLPGIKHAILIGDECQL 600

Query: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYNNQILDAPL 660
            PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSIS FP+SKFY+NQI DAPL
Sbjct: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISYFPSSKFYSNQITDAPL 660

Query: 661  VMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLM 720
            VMD+ +KK +IPSPMFGPY FINVSVGKEEGDDDGHSKKN VEVAVVIKIIEKLY     
Sbjct: 661  VMDEAYKKRYIPSPMFGPYTFINVSVGKEEGDDDGHSKKNAVEVAVVIKIIEKLY----- 720

Query: 721  QLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYE 780
                                      +A RS KT LSIGVISFYAAQVSAIQGRLGQKYE
Sbjct: 721  --------------------------RAWRSVKTRLSIGVISFYAAQVSAIQGRLGQKYE 780

Query: 781  KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINVALTRARHCLWIVG 840
            KS  FTVKVKSVDGFQGGEEDVIILSTVRSNR KNIGFISNSQRINVALTRARHCLWIVG
Sbjct: 781  KSKGFTVKVKSVDGFQGGEEDVIILSTVRSNRRKNIGFISNSQRINVALTRARHCLWIVG 840

Query: 841  DATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSLLFK 900
            DATTLGNSNSEWEAVVS+AK RQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDS+LFK
Sbjct: 841  DATTLGNSNSEWEAVVSDAKDRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSVLFK 900

Query: 901  MAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACGWRPETDYVSNPKCSNIIKCVKV 960
            M QWKVLLSDSFRASFQKVVSINQKKSIIV+LLRL+CGWRPET   SNPKCS+II C KV
Sbjct: 901  MVQWKVLLSDSFRASFQKVVSINQKKSIIVLLLRLSCGWRPETKNFSNPKCSDIINCAKV 960

Query: 961  EGLFIIYSLDIEKDSKYKQILKIWDIKPLTDVKVLVDCLSNIHELYTDDFLNLCKANSHK 1020
            EGL+IIYSLDIEKDS+YKQ+LKIWDIKPLTDVK +VDCLSNIHELYTDDFLNLC ANSHK
Sbjct: 961  EGLYIIYSLDIEKDSEYKQVLKIWDIKPLTDVKGVVDCLSNIHELYTDDFLNLCMANSHK 1020

Query: 1021 GDLELPITWSDSHDIVVYKDHMKAELNAILSLQADTDDTKNITLKKNLLQMKFQSLSYQK 1080
            GDL+LPITWS SHDIVVYKDH+KA+L+AILS Q D+DDTKN TLKKNLLQMKFQSLSYQK
Sbjct: 1021 GDLKLPITWSASHDIVVYKDHIKADLDAILS-QDDSDDTKNATLKKNLLQMKFQSLSYQK 1080

Query: 1081 AKHLLSSRDSKELDLPCQVEDEQLEVILFPTSAFIMGRPGPGKTAALTIKLFMREQQQQI 1140
            AK LLSS DSKELDLPCQVEDEQL++ILFPTSAFIMGRPG GKTAALTIKLFMRE+QQ+I
Sbjct: 1081 AKLLLSSHDSKELDLPCQVEDEQLDIILFPTSAFIMGRPGLGKTAALTIKLFMREKQQEI 1140

Query: 1141 HPEVRCQVTRENAQVTYTMEGGEECKKIDRTVLRQLFITVTLKQCLAVKEHLSYLKR 1193
            HP+   +V R+NA+V+Y  E GEECKKIDRTVLRQLFITVTLKQCLAVKEHL YL R
Sbjct: 1141 HPKGCNKVMRQNAEVSYINESGEECKKIDRTVLRQLFITVTLKQCLAVKEHLLYLSR 1164

BLAST of Spg035353 vs. ExPASy TrEMBL
Match: A0A6J1GWV9 (uncharacterized protein LOC111458260 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458260 PE=4 SV=1)

HSP 1 Score: 1929.1 bits (4996), Expect = 0.0e+00
Identity = 992/1197 (82.87%), Postives = 1055/1197 (88.14%), Query Frame = 0

Query: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYL 60
            ME  GSSK I  KKI FNGLID LFSWTLEDI YDDFYKDKVQ IPESFKSVHQYL SYL
Sbjct: 1    MEPAGSSKMINPKKIRFNGLIDQLFSWTLEDISYDDFYKDKVQNIPESFKSVHQYLASYL 60

Query: 61   FPLLEETRAELSLSLKAIRRAPFAQLVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYR 120
            FPLLEETRAELS SLKAI RAPFA+L+S+EE KSSG+LLLNV+VD+WRN  NN  KEPYR
Sbjct: 61   FPLLEETRAELSSSLKAIHRAPFAKLISVEERKSSGKLLLNVDVDTWRNATNNSKKEPYR 120

Query: 121  TLPGDIFLILDDKPETFMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQK 180
            TLP DIFLILDDKPE  M  Q STRTWAFAWV  +T++GCS+HLKLNVSKNI GE GM K
Sbjct: 121  TLPWDIFLILDDKPENVMNLQCSTRTWAFAWVQNVTDSGCSTHLKLNVSKNIGGEQGMTK 180

Query: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLSTKSMGDKICNKCSLFNNAVCAEKLGS 240
            EFFIVFLMNVTTN+RIWN LHFSED+KIIKHVLS  SMGD+ICNKCSL NN VCAEKLG+
Sbjct: 181  EFFIVFLMNVTTNVRIWNCLHFSEDMKIIKHVLSKNSMGDEICNKCSLSNNVVCAEKLGA 240

Query: 241  NLSSVLNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILA 300
            +LSSVLNDSQK AVLC VCKTLCDHKPSVELIWGPPGTGKTKTISFLLW+ILEMKQR+LA
Sbjct: 241  SLSSVLNDSQKEAVLCCVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWSILEMKQRVLA 300

Query: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLKVGSELEEIYSDYRVD 360
            CAPTNVAITELASRVVKLLRESS+E GVLCSLGD+L+FGNKDRLK+ SELEEIY DYRV 
Sbjct: 301  CAPTNVAITELASRVVKLLRESSKEDGVLCSLGDVLIFGNKDRLKISSELEEIYLDYRVG 360

Query: 361  RLVECFGQSGWKYHITSLIKLLENSNSEYHM-----VNMSRRGKTTGDNEVEVTSFLGFI 420
            +L+ECFGQSGWK HITSLIKLLE+SNSEYH+     VN SR  K  GDN VEV+SFLGFI
Sbjct: 361  KLLECFGQSGWKCHITSLIKLLESSNSEYHIFLESNVNTSRSDKKKGDNGVEVSSFLGFI 420

Query: 421  REKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEILLSQDNVTSVQME 480
            REKFK+TA A+RGCL+TLITHIPKQFILEHNF NIEILLNL+DS   LLSQDNVTS QME
Sbjct: 421  REKFKTTALAVRGCLQTLITHIPKQFILEHNFHNIEILLNLVDSFGTLLSQDNVTSEQME 480

Query: 481  ILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPSTANKKSVRKFCFQ 540
            ILFS  EVFM  FPN S+EATFL+LRSQCLSILR LQASLDQLQLP TANKKSV++FCFQ
Sbjct: 481  ILFSCSEVFMR-FPNYSMEATFLHLRSQCLSILRFLQASLDQLQLPRTANKKSVKQFCFQ 540

Query: 541  RASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGIKHAILIGDECQL 600
            RASLILCTAS+SFQL SMKMDPVNLL+IDEAAQLKECESIVPLQ+PG+KHAILIGDE QL
Sbjct: 541  RASLILCTASSSFQLKSMKMDPVNLLIIDEAAQLKECESIVPLQLPGLKHAILIGDERQL 600

Query: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYNNQILDAPL 660
            PA+VSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFY+NQILDAPL
Sbjct: 601  PAVVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYSNQILDAPL 660

Query: 661  VMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLM 720
            V DKVHKK +I SPMFGPY FINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLY     
Sbjct: 661  VKDKVHKKRYISSPMFGPYTFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLY----- 720

Query: 721  QLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYE 780
                                      KA R AKT L++GVISFYAAQVSAIQ RLG KYE
Sbjct: 721  --------------------------KAWRKAKTRLNVGVISFYAAQVSAIQSRLGHKYE 780

Query: 781  KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINVALTRARHCLWIVG 840
            KSD FTVKVKSVDGFQGGEEDVIIL+TVRSNR  NIGFISNSQRINVALTRARHCLWIVG
Sbjct: 781  KSDNFTVKVKSVDGFQGGEEDVIILTTVRSNRRNNIGFISNSQRINVALTRARHCLWIVG 840

Query: 841  DATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSLLFK 900
            DATTLGNSNSEWE+VVSNAK RQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDS+LFK
Sbjct: 841  DATTLGNSNSEWESVVSNAKDRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSVLFK 900

Query: 901  MAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACGWRPETDYVSNPKCSNIIKCVKV 960
            + QWKVLLSDSFRASFQK+VSINQKKSIIV+LLRLACGWRPE + VSN KCSNII   KV
Sbjct: 901  LVQWKVLLSDSFRASFQKLVSINQKKSIIVLLLRLACGWRPEANSVSNTKCSNIIS-FKV 960

Query: 961  EGLFIIYSLDIEKDSKYKQILKIWDIKPLTDVKVLVDCLSNIHELYTDDFLNLCKANSHK 1020
            EGLFI+YSLDIEKDSKYKQ+LKIWDIKPL DVKVLV+CLSNIHELYTDDFLNLCKA SHK
Sbjct: 961  EGLFIVYSLDIEKDSKYKQVLKIWDIKPLADVKVLVECLSNIHELYTDDFLNLCKAKSHK 1020

Query: 1021 GDLELPITWSDSHDIVVYKDHMKAELNAILSLQADTDDTKNITLKKNLLQMKFQSLSYQK 1080
            GDLELPITWS S D+V+YKDHMKAEL+AILSLQAD+DD KN TLKKNLLQMKFQSLSY K
Sbjct: 1021 GDLELPITWSASLDVVMYKDHMKAELDAILSLQADSDDIKNSTLKKNLLQMKFQSLSYLK 1080

Query: 1081 AKHLLSSRDSKELDLPCQVEDEQLEVILFPTSAFIMGRPGPGKTAALTIKLFMREQQQQI 1140
            AK+LLS  DSKELDLPCQVEDEQLE+ILFPTSAFIMGRP  GKTAALT+KLFMREQQQQI
Sbjct: 1081 AKYLLSRHDSKELDLPCQVEDEQLEIILFPTSAFIMGRPDSGKTAALTMKLFMREQQQQI 1140

Query: 1141 HPEVRCQVTRENAQVTYTMEGGEECKKIDRTVLRQLFITVTLKQCLAVKEHLSYLKR 1193
            H     QVT ENA+V Y  +GGE CKKIDR VLRQLFIT +LK C AVKEHLSYLKR
Sbjct: 1141 HSAGCSQVTIENAEVGYRNDGGEACKKIDRIVLRQLFITASLKHCQAVKEHLSYLKR 1164

BLAST of Spg035353 vs. ExPASy TrEMBL
Match: A0A6J1KCY2 (uncharacterized protein LOC111492119 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492119 PE=4 SV=1)

HSP 1 Score: 1928.3 bits (4994), Expect = 0.0e+00
Identity = 991/1197 (82.79%), Postives = 1056/1197 (88.22%), Query Frame = 0

Query: 1    MEAGGSSKKIKAKKICFNGLIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYL 60
            ME  GSSK I  KKI FNGLID LFSWTLEDI YDDFYKDKVQ IPESF SVHQYL SYL
Sbjct: 1    MEPAGSSKMINPKKIRFNGLIDQLFSWTLEDISYDDFYKDKVQNIPESFNSVHQYLASYL 60

Query: 61   FPLLEETRAELSLSLKAIRRAPFAQLVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYR 120
            FPLLEETRAELS SLKAI RAPFA+L+S+EE KSSG+LLLNV+VD+WRNT NN  KEPYR
Sbjct: 61   FPLLEETRAELSSSLKAIHRAPFAKLISVEERKSSGKLLLNVDVDTWRNTTNNSKKEPYR 120

Query: 121  TLPGDIFLILDDKPETFMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQK 180
            TLPGDIFLILDDKPE  M  Q STRTWAFAWV  +T++GCS+HLKLNVSKNI GE GM K
Sbjct: 121  TLPGDIFLILDDKPENVMNLQCSTRTWAFAWVQNVTDSGCSTHLKLNVSKNIGGEQGMTK 180

Query: 181  EFFIVFLMNVTTNLRIWNSLHFSEDVKIIKHVLSTKSMGDKICNKCSLFNNAVCAEKLGS 240
            EFFIVFLMNVTTN+RIWN LHFSED+KIIKHVL   SMGD+ICNKCSL NN VCAEKLG+
Sbjct: 181  EFFIVFLMNVTTNVRIWNCLHFSEDLKIIKHVLGKNSMGDEICNKCSLSNNVVCAEKLGA 240

Query: 241  NLSSVLNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILA 300
            +LSSVLNDSQK AVLC VCKTLCDHKPSVELIWGPPGTGKTKTISFLLW+IL+MKQR+LA
Sbjct: 241  SLSSVLNDSQKEAVLCCVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWSILKMKQRVLA 300

Query: 301  CAPTNVAITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLKVGSELEEIYSDYRVD 360
            CAPTNVAITEL SRVVKLLRESS+E GVLCSLGD+L+FGNKDRLKV SELEEIY D+RV 
Sbjct: 301  CAPTNVAITELTSRVVKLLRESSKEDGVLCSLGDVLIFGNKDRLKVSSELEEIYLDHRVG 360

Query: 361  RLVECFGQSGWKYHITSLIKLLENSNSEYHM-----VNMSRRGKTTGDNEVEVTSFLGFI 420
            +L++CFGQSGWK HITSLIKLLE+SNSEYH+     VN SR  K  GDN VEV+SFLGFI
Sbjct: 361  KLLKCFGQSGWKCHITSLIKLLESSNSEYHIFLESNVNTSRSDKKQGDNGVEVSSFLGFI 420

Query: 421  REKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEILLSQDNVTSVQME 480
            REKFK+TA A+RGCL+TLITHIPKQFILEHNFQNIEILLNL+DS   LLSQDNVTS QME
Sbjct: 421  REKFKTTALAVRGCLQTLITHIPKQFILEHNFQNIEILLNLVDSFGTLLSQDNVTSEQME 480

Query: 481  ILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPSTANKKSVRKFCFQ 540
            ILFS  EVFM  FP+ S+EATFL+LRSQCLSILR LQASLDQLQLPSTANKKSV++FCFQ
Sbjct: 481  ILFSCSEVFMR-FPDHSMEATFLHLRSQCLSILRFLQASLDQLQLPSTANKKSVKQFCFQ 540

Query: 541  RASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGIKHAILIGDECQL 600
            RASLILCTAS+SFQL SMKMDPVNLL+IDEAAQLKECESIVPLQ+PG+KHAILIGDE QL
Sbjct: 541  RASLILCTASSSFQLKSMKMDPVNLLIIDEAAQLKECESIVPLQLPGLKHAILIGDERQL 600

Query: 601  PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYNNQILDAPL 660
            PA+VSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFY+NQILDAPL
Sbjct: 601  PAVVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYSNQILDAPL 660

Query: 661  VMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLM 720
            V DKVHKK +I SPMFGPY FINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLY     
Sbjct: 661  VKDKVHKKRYISSPMFGPYTFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLY----- 720

Query: 721  QLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYE 780
                                      KA R AKT L++GVISFYAAQVSAIQ RLG KYE
Sbjct: 721  --------------------------KAWRKAKTRLNVGVISFYAAQVSAIQSRLGHKYE 780

Query: 781  KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINVALTRARHCLWIVG 840
            KSD FTVKVKSVDGFQGGEEDVIIL+TVRSNR  NIGFISNSQRINVALTRARHCLWIVG
Sbjct: 781  KSDNFTVKVKSVDGFQGGEEDVIILTTVRSNRRNNIGFISNSQRINVALTRARHCLWIVG 840

Query: 841  DATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNKDSLLFK 900
            DATTLGNSNSEWE+VVSNAK RQCYFNAEEDKDLADAIIEVKKVLLELDDLLN+DS+LFK
Sbjct: 841  DATTLGNSNSEWESVVSNAKDRQCYFNAEEDKDLADAIIEVKKVLLELDDLLNQDSVLFK 900

Query: 901  MAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACGWRPETDYVSNPKCSNIIKCVKV 960
            + QWKVLLSDSFRASFQKVVSINQKKSIIV+LLRLACGWRPE + VSNPKCSNII  VKV
Sbjct: 901  LVQWKVLLSDSFRASFQKVVSINQKKSIIVLLLRLACGWRPEANSVSNPKCSNIIS-VKV 960

Query: 961  EGLFIIYSLDIEKDSKYKQILKIWDIKPLTDVKVLVDCLSNIHELYTDDFLNLCKANSHK 1020
            EGLFI+YSLDIEKD KYKQ+LKIWDIKPL DVKVLV+CLSNIHELYTDDFLNLCKA SHK
Sbjct: 961  EGLFIVYSLDIEKDLKYKQVLKIWDIKPLADVKVLVECLSNIHELYTDDFLNLCKAKSHK 1020

Query: 1021 GDLELPITWSDSHDIVVYKDHMKAELNAILSLQADTDDTKNITLKKNLLQMKFQSLSYQK 1080
            GDLELPITW  S D+V+YKDHMKAEL+AILSLQAD+DD KN TLKKNLLQMKFQSLSY K
Sbjct: 1021 GDLELPITWGASLDVVIYKDHMKAELDAILSLQADSDDIKNGTLKKNLLQMKFQSLSYLK 1080

Query: 1081 AKHLLSSRDSKELDLPCQVEDEQLEVILFPTSAFIMGRPGPGKTAALTIKLFMREQQQQI 1140
            AKHLLS   SKELDLPCQVEDEQLE+ILFPTSAFIMGRP   KTAALTIKLFMRE+QQQI
Sbjct: 1081 AKHLLSRHASKELDLPCQVEDEQLEIILFPTSAFIMGRPDSRKTAALTIKLFMRERQQQI 1140

Query: 1141 HPEVRCQVTRENAQVTYTMEGGEECKKIDRTVLRQLFITVTLKQCLAVKEHLSYLKR 1193
            H     QV R+NA+V Y  +GGE CKKIDRTVLRQLFIT TLKQC AVKEHLSYLKR
Sbjct: 1141 HSGGCSQVMRDNAEVGYRNDGGEACKKIDRTVLRQLFITATLKQCQAVKEHLSYLKR 1164

BLAST of Spg035353 vs. TAIR 10
Match: AT1G65810.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 606.7 bits (1563), Expect = 4.1e-173
Identity = 407/1109 (36.70%), Postives = 608/1109 (54.82%), Query Frame = 0

Query: 9    KIKAKKICFNGLIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYLFPLLEETR 68
            K K K I    L+D +FSW+L D+L  + Y+ +V KIP +F S  +Y  S++ P++EET 
Sbjct: 11   KKKEKIIKGRDLVDVVFSWSLRDVLNSNLYRGQVGKIPNTFTSTKEYFESFVKPIIEETH 70

Query: 69   AELSLSLKAIRRA---PFAQLVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYRTLPGD 128
            A+L  S+  IRRA    F ++   ++ K    L   V +          G+        D
Sbjct: 71   ADLLSSMGTIRRAQAFKFWEIKPGKDFKPPRDLYYEVTLQMTNEYMTKGGQNLLEV--ND 130

Query: 129  IFLILDDKPETFMISQYSTRTWAFAWVNQITENGCSSHL-KLNVSKNI----------SG 188
            +  + D +P      ++S   +  A V  + EN  + HL  +  SK I          S 
Sbjct: 131  LIAVTDKRPIRIDDLRFSHEPYLLALVCGVNEN--NPHLITILASKPIIFDDDDDIKTSS 190

Query: 189  EHGMQK----EFFIVFLMNVTTNLRIWNSLHFSED---VKIIKHVL-STKSMGDKICNKC 248
            + G  +     FF V L+N+ TN+RIW +LH + +   +K+I  VL S   +    C  C
Sbjct: 191  KRGKGERKSLSFFGVNLINMMTNIRIWTALHPNPEGGNLKLISRVLQSNNEVDGGSCVSC 250

Query: 249  SLFNNAVCAEKLGSNLSSV-LNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTIS 308
               + +V ++     L S  LN SQ+ A+L  +    C+H  +++LIWGPPGTGKTKT S
Sbjct: 251  KENSESVVSDYSARMLRSFKLNSSQEDAILRCLEAKSCNHSNNIKLIWGPPGTGKTKTTS 310

Query: 309  FLLWAILEMKQRILACAPTNVAITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLK 368
             LL   L+M+ R L CAPTN+A+ E+ SR+VKL+ ES R  G    LGD++LFGNK+R+K
Sbjct: 311  VLLLNFLKMRCRTLTCAPTNIAVLEVCSRLVKLVSESLRFDGY--GLGDIVLFGNKERMK 370

Query: 369  VG--SELEEIYSDYRVDRLVECF-GQSGWKYHITSLIKLLENSNSEYHMVNMSRRGKTTG 428
            +    +L +++ +YRVD L  CF   +GW+ ++  +I LL +   E+      R+ K+  
Sbjct: 371  IDDREDLFDVFLEYRVDELYRCFMALTGWRANVNRMICLLSDPKHEF------RQFKSV- 430

Query: 429  DNEVEVTSFLGFIREKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEI 488
                 + SF  F+ E+       L     TL  H+P   +     + +    NL+ ++  
Sbjct: 431  --NTTLLSFKDFVEERLSRLRYDLHHQFTTLCLHLPTSLLSFRVAEKMNQTNNLLRNI-- 490

Query: 489  LLSQDNVTSVQMEILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPS 548
                    S  M   +   +  + D  + +   T       CL +L  +  S   ++LP 
Sbjct: 491  ------AASDVMRDGYGRMKYKLKDTGDENDSRT-----QDCLEMLTSISMS---IKLPD 550

Query: 549  TANKKSVRKFCFQRASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPG 608
              +K  ++K C   A L+ CTAS+S +L+     P+ LLVIDEAAQLKECES +PLQ+ G
Sbjct: 551  FISKFELQKLCLDNAYLLFCTASSSARLH--MSSPIQLLVIDEAAQLKECESAIPLQLRG 610

Query: 609  IKHAILIGDECQLPAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPN 668
            ++HAILIGDE QLPA++ S +   A  GRSLFERL LLGH+K LLN QYRMHPSIS FPN
Sbjct: 611  LQHAILIGDEKQLPAMIKSNIASEADLGRSLFERLVLLGHNKQLLNMQYRMHPSISIFPN 670

Query: 669  SKFYNNQILDAPLVMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVV 728
             +FY+ +ILDAP V  + ++K F+P  M+GPY FIN++ G+E+   +G+S KN VEV+VV
Sbjct: 671  REFYDMKILDAPSVRLRSYEKKFLPEKMYGPYSFINIAYGREQ-FGEGYSSKNLVEVSVV 730

Query: 729  IKIIEKLYKGMLMQLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQ 788
             +I+ KLY                              + +R++ +T +S+GVIS Y AQ
Sbjct: 731  AEIVSKLY------------------------------SVSRKTGRT-ISVGVISPYKAQ 790

Query: 789  VSAIQGRLGQKYEKSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINV 848
            V AIQ R+G+KY     FTV V+SVDGFQGGEED+II+STVRSN    IGF+SN QR NV
Sbjct: 791  VFAIQERIGEKYNTEGTFTVSVRSVDGFQGGEEDIIIISTVRSNGNGAIGFLSNQQRTNV 850

Query: 849  ALTRARHCLWIVGDATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIEVKKVLLE 908
            ALTRAR+CLWI+G+  TL N+ S W  +V +AK R C+ NAEED+ LA  I      L +
Sbjct: 851  ALTRARYCLWILGNEATLTNNRSVWRQLVDDAKARNCFHNAEEDESLAQCIERSTTALDD 910

Query: 909  LDDLLNKDSLLFKMAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACG--WRPETDY 968
            L+ L NK  + F+ + WKV LS  F  S + +V     K ++  L +L+ G     E ++
Sbjct: 911  LNKLQNKKLISFENSIWKVWLSYEFLKSLETIVDSEINKRVMSFLEKLSNGKELHQEVEF 970

Query: 969  VSNPKCSNIIKCVKV-EGLFIIYSLDIEK-DSKYKQILKIWDIKPLTDVKVLVDCLSNIH 1028
             S     N+++  +  +GL +I+++DI K ++++ Q+LKIW + P TDV  + + L   +
Sbjct: 971  ES----ENLLRQHEFDDGLSLIWAIDIFKNNNQHVQVLKIWQVLPSTDVSRVTEHLEKHY 1030

Query: 1029 ELYTDDFLNLCKANSHKGDLELPITWSDSHDIVVYKDHMKAELNAILSLQADTDDTKNIT 1088
              YT   ++ C+    +GDL +P+ W    +    KD +     +   L    ++T  ++
Sbjct: 1031 RRYTKGKISRCRYICSQGDLVVPMQWPVDSNSCSKKDIVSDVSRSFALLSVVEEET--VS 1048

BLAST of Spg035353 vs. TAIR 10
Match: AT1G65780.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 578.6 bits (1490), Expect = 1.2e-164
Identity = 379/1055 (35.92%), Postives = 574/1055 (54.41%), Query Frame = 0

Query: 20   LIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYLFPLLEETRAELSLSLKAIR 79
            L+D + SW+L+++L  D YK +V+KIP  F+S   Y  +++ PL+EET A L  S++ + 
Sbjct: 11   LVDLVLSWSLDEVLNVDLYKGQVEKIPMEFESTGDYFKTFIPPLIEETHAALLSSMRKLW 70

Query: 80   RAPFAQ---LVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYRTLPGDIFLILDDKPET 139
            +AP  +   ++   E K    L   V +    N A+       + +P D+  + D +P  
Sbjct: 71   QAPVVEISYIMQTAEYKLPNDLFYKVRLSGISNEAST------KLMPRDLISLTDQRPNH 130

Query: 140  FMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQKE------FFIVFLMNV 199
                  S+  +  A V ++  +   + + +  SK +  E G +K+       F + L+N+
Sbjct: 131  VDGFNISSEPYIVALVCKVDPDR-PNDVTILASKPLFVEDGRRKKNEKKERLFGIHLVNL 190

Query: 200  TTNLRIWNSLHFSED---VKIIKHVLSTKSMGDKICNKCSLFNNAVCAEKLGSNLSSVLN 259
            TTN+RIWN+LH  ++   + +I  VL   S  +  C +C        ++ L       LN
Sbjct: 191  TTNIRIWNALHPGDEGVNLNLISRVLRRNSEDEGFCIQCLQEG----SDGLAPRRFLKLN 250

Query: 260  DSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILACAPTNVA 319
             SQ+ A+L  +    C H  +V LIWGPPGTGKTKT S LL+ +L  K R L C PTNV+
Sbjct: 251  PSQEDAILNCLDVRRCYHANTVRLIWGPPGTGKTKTTSVLLFTLLNAKCRTLTCGPTNVS 310

Query: 320  ITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLKV--GSELEEIYSDYRVDRLVEC 379
            + E+ASRV+KL+  S + G     LGD++LFGN +R+K+    +L  I+ D RVD+L  C
Sbjct: 311  VLEVASRVLKLVSGSLKIGNY--GLGDVVLFGNDERMKIKDRKDLVNIFIDERVDKLYPC 370

Query: 380  FGQ-SGWKYHITSLIKLLENSNSEY--HMVNMS---------------RRGKTTGDNEVE 439
            F    GWK  I  +I+LLE+   +Y  ++ N++               R+G    +N VE
Sbjct: 371  FMPFYGWKATIDGMIRLLEDPKGQYNLYLENLARANNVKRKDTGSVFKRKGNEQNENIVE 430

Query: 440  VT------SFLGFIREKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLE 499
                    SF  ++ EKF      L     +L TH+P   +       +   ++L+  + 
Sbjct: 431  QVSDTRPQSFQDYLPEKFSELRKDLDLHFSSLCTHLPTALLSSQAATRMYEAIDLVRDVT 490

Query: 500  ILLSQDNVTSVQMEILFSSPEVFMMDFPNSSVEATFLYLRSQCLSI----LRILQASLDQ 559
            IL   D VT   ++ +           PN      F    SQ +++    L++L++  + 
Sbjct: 491  ILAILDGVTGEGVKSVL---------IPNGEGSDRF---SSQHVTVEDDYLKLLRSIPEI 550

Query: 560  LQLPSTANKKSVRKFCFQRASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVP 619
              LP+ +++  +++ C   A L+  TAS S +L +    P+ LLVIDEAAQLKECES +P
Sbjct: 551  FPLPAVSDRHLIKELCLGHACLLFSTASCSARLYT--GTPIQLLVIDEAAQLKECESSIP 610

Query: 620  LQVPGIKHAILIGDECQLPAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSI 679
            +Q+PG++H IL+GDE QLPA+V SQ+   AG+GRSLFERL+LLGH K++LN QYRMH SI
Sbjct: 611  MQLPGLRHLILVGDERQLPAMVESQIALEAGFGRSLFERLALLGHKKYMLNIQYRMHCSI 670

Query: 680  SCFPNSKFYNNQILDAPLVMDKVHKKSFIPSPMFGPYVFINVSVGKEE-GDDDGHSKKNT 739
            S FPN + Y  +ILDAP V  + + K ++P  M+GPY FIN++ G+EE G+ +G S KN 
Sbjct: 671  SSFPNKELYGKKILDAPTVRQRNYTKQYLPGEMYGPYSFINIAYGREEYGEGEGRSLKNN 730

Query: 740  VEVAVVIKIIEKLYKGMLMQLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVI 799
            VEV VV  II       L+Q+  K                           KT +++GVI
Sbjct: 731  VEVVVVAAIIAN-----LLQVSEK--------------------------TKTRINVGVI 790

Query: 800  SFYAAQVSAIQGRLGQKY--EKSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFI 859
            S Y AQV AIQ ++ +    +    F++++++VDGFQGGEED+II+STVRSN    +GF+
Sbjct: 791  SPYKAQVIAIQEKIQETSIGDAGGLFSLRIRTVDGFQGGEEDIIIVSTVRSNGVGRVGFL 850

Query: 860  SNSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAII 919
             N +R NV LTRAR CLWI+G+  TL NS S W  ++ +AK R C+ +A ED+ LA AI 
Sbjct: 851  GNRRRTNVLLTRARFCLWILGNEATLMNSKSVWRNLIQDAKERGCFHSAGEDESLAQAIA 910

Query: 920  EVKKVLLELDDLLNKDSLLFKMAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACGW 979
                  +E   L N        ++WK+  SD F+    ++ +    + I   L RL+ GW
Sbjct: 911  STN---IEFRPLNN--------SKWKLCFSDEFKKYVGEIKNPETYRKIKNFLERLSQGW 970

Query: 980  --RPETDYVSNPKCSNIIKCVKVEG-LFIIYSLDI-EKDSKYKQILKIWDIKPLTDVKVL 1026
                ET+  +    S ++K  K++  L II+++DI ++D  Y Q+LKIWD+ P +D    
Sbjct: 971  LKEEETERENLVSSSQLLKQSKIDDVLRIIWAVDILKEDFHYDQVLKIWDVVPSSDAPEA 996

BLAST of Spg035353 vs. TAIR 10
Match: AT1G65810.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 567.0 bits (1460), Expect = 3.6e-161
Identity = 368/951 (38.70%), Postives = 530/951 (55.73%), Query Frame = 0

Query: 9   KIKAKKICFNGLIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYLFPLLEETR 68
           K K K I    L+D +FSW+L D+L  + Y+ +V KIP +F S  +Y  S++ P++EET 
Sbjct: 11  KKKEKIIKGRDLVDVVFSWSLRDVLNSNLYRGQVGKIPNTFTSTKEYFESFVKPIIEETH 70

Query: 69  AELSLSLKAIRRA---PFAQLVSIEEPKSSGRLLLNVNVDSWRNTANNIGKEPYRTLPGD 128
           A+L  S+  IRRA    F ++   ++ K    L   V +          G+        D
Sbjct: 71  ADLLSSMGTIRRAQAFKFWEIKPGKDFKPPRDLYYEVTLQMTNEYMTKGGQNLLEV--ND 130

Query: 129 IFLILDDKPETFMISQYSTRTWAFAWVNQITENGCSSHL-KLNVSKNI----------SG 188
           +  + D +P      ++S   +  A V  + EN  + HL  +  SK I          S 
Sbjct: 131 LIAVTDKRPIRIDDLRFSHEPYLLALVCGVNEN--NPHLITILASKPIIFDDDDDIKTSS 190

Query: 189 EHGMQK----EFFIVFLMNVTTNLRIWNSLHFSED---VKIIKHVL-STKSMGDKICNKC 248
           + G  +     FF V L+N+ TN+RIW +LH + +   +K+I  VL S   +    C  C
Sbjct: 191 KRGKGERKSLSFFGVNLINMMTNIRIWTALHPNPEGGNLKLISRVLQSNNEVDGGSCVSC 250

Query: 249 SLFNNAVCAEKLGSNLSSV-LNDSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTIS 308
              + +V ++     L S  LN SQ+ A+L  +    C+H  +++LIWGPPGTGKTKT S
Sbjct: 251 KENSESVVSDYSARMLRSFKLNSSQEDAILRCLEAKSCNHSNNIKLIWGPPGTGKTKTTS 310

Query: 309 FLLWAILEMKQRILACAPTNVAITELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLK 368
            LL   L+M+ R L CAPTN+A+ E+ SR+VKL+ ES R  G    LGD++LFGNK+R+K
Sbjct: 311 VLLLNFLKMRCRTLTCAPTNIAVLEVCSRLVKLVSESLRFDGY--GLGDIVLFGNKERMK 370

Query: 369 VG--SELEEIYSDYRVDRLVECF-GQSGWKYHITSLIKLLENSNSEYHMVNMSRRGKTTG 428
           +    +L +++ +YRVD L  CF   +GW+ ++  +I LL +   E+      R+ K+  
Sbjct: 371 IDDREDLFDVFLEYRVDELYRCFMALTGWRANVNRMICLLSDPKHEF------RQFKSV- 430

Query: 429 DNEVEVTSFLGFIREKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEI 488
                + SF  F+ E+       L     TL  H+P   +     + +    NL+ ++  
Sbjct: 431 --NTTLLSFKDFVEERLSRLRYDLHHQFTTLCLHLPTSLLSFRVAEKMNQTNNLLRNI-- 490

Query: 489 LLSQDNVTSVQMEILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPS 548
                   S  M   +   +  + D  + +   T       CL +L  +  S   ++LP 
Sbjct: 491 ------AASDVMRDGYGRMKYKLKDTGDENDSRT-----QDCLEMLTSISMS---IKLPD 550

Query: 549 TANKKSVRKFCFQRASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPG 608
             +K  ++K C   A L+ CTAS+S +L+     P+ LLVIDEAAQLKECES +PLQ+ G
Sbjct: 551 FISKFELQKLCLDNAYLLFCTASSSARLH--MSSPIQLLVIDEAAQLKECESAIPLQLRG 610

Query: 609 IKHAILIGDECQLPAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPN 668
           ++HAILIGDE QLPA++ S +   A  GRSLFERL LLGH+K LLN QYRMHPSIS FPN
Sbjct: 611 LQHAILIGDEKQLPAMIKSNIASEADLGRSLFERLVLLGHNKQLLNMQYRMHPSISIFPN 670

Query: 669 SKFYNNQILDAPLVMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVV 728
            +FY+ +ILDAP V  + ++K F+P  M+GPY FIN++ G+E+   +G+S KN VEV+VV
Sbjct: 671 REFYDMKILDAPSVRLRSYEKKFLPEKMYGPYSFINIAYGREQ-FGEGYSSKNLVEVSVV 730

Query: 729 IKIIEKLYKGMLMQLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQ 788
            +I+ KLY                              + +R++ +T +S+GVIS Y AQ
Sbjct: 731 AEIVSKLY------------------------------SVSRKTGRT-ISVGVISPYKAQ 790

Query: 789 VSAIQGRLGQKYEKSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINV 848
           V AIQ R+G+KY     FTV V+SVDGFQGGEED+II+STVRSN    IGF+SN QR NV
Sbjct: 791 VFAIQERIGEKYNTEGTFTVSVRSVDGFQGGEEDIIIISTVRSNGNGAIGFLSNQQRTNV 850

Query: 849 ALTRARHCLWIVGDATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIEVKKVLLE 908
           ALTRAR+CLWI+G+  TL N+ S W  +V +AK R C+ NAEED+ LA  I      L +
Sbjct: 851 ALTRARYCLWILGNEATLTNNRSVWRQLVDDAKARNCFHNAEEDESLAQCIERSTTALDD 896

Query: 909 LDDLLNKDSLLFKMAQWKVLLSDSFRASFQKVVSINQKKSIIVILLRLACG 934
           L+ L NK  + F+ + WKV LS  F  S + +V     K ++  L +L+ G
Sbjct: 911 LNKLQNKKLISFENSIWKVWLSYEFLKSLETIVDSEINKRVMSFLEKLSNG 896

BLAST of Spg035353 vs. TAIR 10
Match: AT5G37150.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 545.8 bits (1405), Expect = 8.6e-155
Identity = 333/882 (37.76%), Postives = 510/882 (57.82%), Query Frame = 0

Query: 20  LIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYLFPLLEETRAELSLSLKAIR 79
           L+D +FSW+++DIL  DFYK K   +P+ F+SV +Y   ++  LL E   EL  SLK++ 
Sbjct: 9   LVDRVFSWSIKDILNKDFYKQKT--VPDKFRSVDEYYQCFVPHLLIEAHTELFSSLKSVS 68

Query: 80  RAPFAQLVSIE------EPKSSGRLLLNVNVDSWRNTANNIGKEPYRTLPGDIFLILDDK 139
           ++PF Q+ S+E         SS +L  ++ + +  + +       Y+   GD+  +  DK
Sbjct: 69  KSPFVQIRSMETKTKQSSGSSSNKLFYDITLKATESLS-----AKYQPKCGDLIALTMDK 128

Query: 140 PETFMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQKEFFIVFLMNVTTN 199
           P    I+  +    A+     +  +     + +++S++IS    ++   F VFLM +TTN
Sbjct: 129 PR--RINDLNPLLLAY-----VFSSDGDLKISVHLSRSIS---PLENYSFGVFLMTLTTN 188

Query: 200 LRIWNSLHFSEDVK-IIKHVLSTKSMGD----KICNKCSLFNNAVCAEKLGSNLSSVLND 259
            RIWN+LH    +  + K VL   ++ +    K+    +LF + + + K        LN 
Sbjct: 189 TRIWNALHNEAAISTLTKSVLQANTVNNVFVLKMMGDLTLFLDIIRSTK--------LNS 248

Query: 260 SQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILACAPTNVAI 319
           SQ+ A+L  +    C HK SV+LIWGPPGTGKTKT++ LL+A+L+++ + + CAPTN AI
Sbjct: 249 SQEDAILGCLETRNCTHKNSVKLIWGPPGTGKTKTVATLLFALLKLRCKTVVCAPTNTAI 308

Query: 320 TELASRVVKLLRESSREGGVLCSLGDMLLFGNKDRLKVGSE---LEEIYSDYRVDRLVEC 379
            ++ASR++ L +E+S        LG+++L GN+DR+ +      L +++ D R+ +L + 
Sbjct: 309 VQVASRLLSLFKENSTSENATYRLGNIILSGNRDRMGIHKNDHVLLDVFLDERIGKLGKL 368

Query: 380 FGQ-SGWKYHITSLIKLLENSNSEY--HMVNMSRRGKTTGDNE-----VEVTSFLGFIRE 439
           F   SGW   + SLI+ LEN   +Y  H+  +    +   + E     V + +   F+++
Sbjct: 369 FSPFSGWMQRLESLIQFLENPEGKYERHVYELEEVERMEEEAERQEVVVNIPTIGEFVKK 428

Query: 440 KFKSTASALRGCLRTLITHIPKQFILEHNFQNIEILLNLIDSLEIL--LSQDNVTSVQME 499
            F S +  +  C+  L TH+PK ++    + +++I++    SL+ +    ++N + V  E
Sbjct: 429 NFNSLSEEVETCIVDLFTHLPKVYL---PYDDVKIMIASRQSLQRIRYFLRENSSRVDFE 488

Query: 500 ILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQASLDQLQLPSTANKKSVRKFCFQ 559
                   F  D         F  L   CL  LR+L     + ++P     + +RKFC Q
Sbjct: 489 -----EGNFRFD--------CFKRLSVDCLKALRLLP---KRFEIPDMLENEDIRKFCLQ 548

Query: 560 RASLILCTASTSFQLNSMKMDPVNLLVIDEAAQLKECESIVPLQVPGIKHAILIGDECQL 619
            A +ILCTAS + ++N  +   V LLV+DEAAQLKECES+  LQ+PG++HAILIGDE QL
Sbjct: 549 NADIILCTASGAAEMNVERTGNVELLVVDEAAQLKECESVAALQLPGLRHAILIGDEFQL 608

Query: 620 PAIVSSQVCDAAGYGRSLFERLSLLGHSKHLLNTQYRMHPSISCFPNSKFYNNQILDAPL 679
           PA+V +++C+ A +GRSLFERL LLGH+KHLL+ QYRMHPSIS FPN +FY  +I DA  
Sbjct: 609 PAMVHNEMCEKAKFGRSLFERLVLLGHNKHLLDVQYRMHPSISRFPNKEFYGGRIKDAEN 668

Query: 680 VMDKVHKKSFIPSPMFGPYVFINVSVGKEEGDDDGHSKKNTVEVAVVIKIIEKLYKGMLM 739
           V + +++K F+   MFG + FINV  GKEE   DGHS KN VEVAVV +II  L+K    
Sbjct: 669 VKESIYQKRFLQGNMFGSFSFINVGRGKEE-FGDGHSPKNMVEVAVVSEIISNLFK---- 728

Query: 740 QLGHKWYFREEHGISFHQGAKPLSLNKARRSAKTSLSIGVISFYAAQVSAIQGRLGQKYE 799
                        +S  +  K              +S+GV+S Y  Q+ AIQ ++G KY 
Sbjct: 729 -------------VSCERRMK--------------VSVGVVSPYKGQMRAIQEKIGDKYS 788

Query: 800 --KSDKFTVKVKSVDGFQGGEEDVIILSTVRSNRGKNIGFISNSQRINVALTRARHCLWI 859
                +F + V+SVDGFQGGEED+II+STVRSN    +GF++N QR NVALTRARHCLW+
Sbjct: 789 SLSGQQFALNVRSVDGFQGGEEDIIIISTVRSNSNGKVGFLNNRQRANVALTRARHCLWV 814

Query: 860 VGDATTLGNSNSEWEAVVSNAKYRQCYFNAEEDKDLADAIIE 876
           +G+ TTL  S S W  ++S ++ R C+++A ++ +L +A+ E
Sbjct: 849 IGNETTLALSGSIWATLISESRTRGCFYDATDEMNLRNAMNE 814

BLAST of Spg035353 vs. TAIR 10
Match: AT5G37160.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 502.7 bits (1293), Expect = 8.3e-142
Identity = 329/910 (36.15%), Postives = 485/910 (53.30%), Query Frame = 0

Query: 20  LIDHLFSWTLEDILYDDFYKDKVQKIPESFKSVHQYLGSYLFPLLEETRAELSLSLKAIR 79
           L   L SW+L+DIL +D  K+K+  IP+ F SV +Y   ++  LLEETR EL  S +++ 
Sbjct: 18  LFARLCSWSLKDILNEDLSKEKIMTIPDRFSSVDEYSQCFVPHLLEETRTELFSSFRSLS 77

Query: 80  RAPFAQLVSIE------EPKSSGRLLLNVNVDSWRNTANNIGKEPYRTLPGDIFLI--LD 139
           ++P ++++S+E        +SS +   ++ +  + +  N I    Y    GDI  +  L 
Sbjct: 78  KSPVSRILSVETKVIEYSGRSSIKWFHDIKLMDYADDKNEI----YEPKCGDIIALSPLS 137

Query: 140 DKPETFMISQYSTRTWAFAWVNQITENGCSSHLKLNVSKNISGEHGMQKEFFI--VFLMN 199
              E   I         + + +   ++  S H   ++S++       +K  F   VFL+N
Sbjct: 138 LTEERPRIDDLDPLLLGYVF-SVYGDSKISVHFSRSISQS-------EKHTFCTGVFLIN 197

Query: 200 VTTNLRIWNSLH-FSEDVKIIKHVLSTKSMGDKICNKCSLFNNAVCAEKLGSNL-SSVLN 259
           +TTN RIWN+LH  + D  +I+ VL   +   + C  C    +   ++++   + S+ LN
Sbjct: 198 ITTNTRIWNALHKDAADSTLIQSVLQEDASATEQCFSCENDVDGSDSDRVVDIIRSAKLN 257

Query: 260 DSQKSAVLCSVCKTLCDHKPSVELIWGPPGTGKTKTISFLLWAILEMKQRILACAPTNVA 319
            SQ++A+L  +    C HK SV+LIWGPPGTGKTKT++ LL  ++++K + + CAPTN  
Sbjct: 258 SSQEAAILGFLKTRNCKHKESVKLIWGPPGTGKTKTVATLLSTLMQLKCKTVVCAPTNTT 317

Query: 320 ITELASRVVKLLRESSREGGVLCS-------------------------------LGDML 379
           I  +ASR++ L +E+     ++C+                               +G+++
Sbjct: 318 IVAVASRLLSLSKET-----IVCAPTNSAIAEVVSRFEFSTLFYGTSILERTTYGMGNIV 377

Query: 380 LFGNKDRLKVGSE--LEEIYSDYRVDRLVECF-GQSGWKYHITSLIKLLENSNSEYHM-V 439
           L GN++R+ + S   L  ++ + RV +L   F    GWK  + S+I  LEN+ ++Y   V
Sbjct: 378 LSGNRERMGITSNKVLLNVFFNDRVSKLGRLFLSTCGWKKRLESIIDFLENTETKYEQHV 437

Query: 440 NMSRRGKTTGDNEVEVTSFLGFIREKFKSTASALRGCLRTLITHIPKQFILEHNFQNIEI 499
           N     + T D + +       + E+           +  L TH+PK FI   + +N+  
Sbjct: 438 NELELERMTEDEKKKEE-----VEERTMQEVD-----MADLSTHLPKSFISSKDVKNLIA 497

Query: 500 LLNLIDSLEILLSQDNVTSVQMEILFSSPEVFMMDFPNSSVEATFLYLRSQCLSILRILQ 559
               +  +   L +++                  DF            R  C + L  + 
Sbjct: 498 ACQALHRVRYFLQENSSRD---------------DFKKGG-------FRFNCFNKLISVD 557

Query: 560 ASLDQLQLP------STANKKSVRKFCFQRASLILCTASTSFQLNSMKMDPVNLLVIDEA 619
           A      LP        AN + +RKFC Q A +I CTAS+   +N  ++  V+LLV+DE 
Sbjct: 558 ALQALCLLPKCFGIFGLANNEDIRKFCLQNADIIFCTASSVANINPARIGSVDLLVVDET 617

Query: 620 AQLKECESIVPLQVPGIKHAILIGDECQLPAIVSSQVCDAAGYGRSLFERLSLLGHSKHL 679
           AQLKECES+  LQ+PG+ HA+LIGDE QLPA+V ++ CD A +GRSLFERL L+GHSKHL
Sbjct: 618 AQLKECESVAALQLPGLCHALLIGDEYQLPAMVHNEECDKAKFGRSLFERLVLIGHSKHL 677

Query: 680 LNTQYRMHPSISCFPNSKFYNNQILDAPLVMDKVHKKSFIPSPMFGPYVFINVSVGKEEG 739
           LN QYRMHPSIS FPN +FY  +I DA  V + +++K F+   MFG + FINV  GKEE 
Sbjct: 678 LNVQYRMHPSISRFPNKEFYGGRITDAANVQESIYEKRFLQGNMFGTFSFINVGRGKEE- 737

Query: 740 DDDGHSKKNTVEVAVVIKIIEKLYKGMLMQLGHKWYFREEHGISFHQGAKPLSLNKARRS 799
             DGHS KN VEVAV+ KII  L+                               K    
Sbjct: 738 FGDGHSPKNMVEVAVISKIISNLF-------------------------------KVSSQ 797

Query: 800 AKTSLSIGVISFYAAQVSAIQGRLGQKYEK---SDKFTVKVKSVDGFQGGEEDVIILSTV 859
            K  +S+GVIS Y  QV AIQ R+G KY        FT+ V+SVDGFQGGE DVII+STV
Sbjct: 798 RKQKMSVGVISPYKGQVRAIQERVGDKYNSLSVDQLFTLNVQSVDGFQGGEVDVIIISTV 846

Query: 860 RSNRGKNIGFISNSQRINVALTRARHCLWIVGDATTLGNSNSEWEAVVSNAKYRQCYFNA 874
           R N   N+GF+SN QR NVALTRARHCLW++G+ TTL  S S W  ++S ++ R C+++A
Sbjct: 858 RCNVNGNVGFLSNRQRANVALTRARHCLWVIGNGTTLALSGSIWAELISESRTRGCFYDA 846