Spg035332 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg035332
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Thiamine-phosphate pyrophosphorylase
Location	scaffold7: 22296727 .. 22302630 (+)
RNA-Seq Expression	Spg035332
Synteny	Spg035332

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTAAGGTAGACATGTAGAAAATAGGAACTCTAGACAAATGCCACAAATCCATGAGGAAGGGTATCGGAACTCCAATGGAAGGAGAGAGTTCTTCATTTGGCAAGTTTTCCCAAGGTCATTTGCTCAACCAATTTCTAGAAATTAGAAAATTTTGTATAGTGATTAGAAAATTAAAACTAATTTAATGCCTATTCTCTAAAAATGTGAATGTTCACCTATGTAAATCCTAATAACATCATGATCTACATTCATGTTTTAGATGATGTCCATACATCAAAGTGAGGACTGTAGGCAACTCCCAACAATTTGATATAGTTTTTTGTGGTATTACTACTCTTTTCACTTTCACATGTTAGATTGGAAGGCTTTTACGAAAGTCCTCTGCTTGGAAAAGGGACATCTTGTCCCTTTGTCCGTCAGCTGCTTTGGAAGCTTTCCTGAATGGAATTTCTGGCATGTTTATTATAAGAAGAGAAGAGAGGGAGAGAGAGAGAAAAAAAGAAAACATCTTGGTTGTAGCTTCCCTTGATAGTTACTGGGGTCTTATTTTGTTAGATTGGAAACCCTTCATTTAGTTGGGTTCTCCTTTGGGGCTTGGTTTTTTGTATGCCCTTGTATTCTTTCATTTTTTTTCTCAATGAAAGCTCGGTTTCTTATCAAAAAAAAAGTTGCTGGTTTCTCTTGTTGAGAGAGGCTTTAGTTTTGACTTGGCAAAACTTGCTAGTCTTTATTTGGCTACATGTTGCTCCGACAAAATTGCCTCTTCTTGGCCCCTAAGTGCCCTTGTTGGTAAGAAATTACAATCTTAGAACCCCTCCCCAAGTCTTTAATTCCAACCGTGGGTCCTAGGGGTCAAGAGAATTAACTTCCCTACCCATATGGGTAGGGACCCATTGAGATCTACTAGAACTGGAGCAGGTGGGTTTCCCTGGGGATTAGAGGGGGTGGGACCCATATGCCCCAGTGTTTGGATGAAAATAGTGCTTTTCCATTATTCCAATTTCATGTTTGGTCTTAATATGTTAGTTTTTCATAAATCTATGACACGTCTCTTGCTTGCAGGGAAGAACTTCTACCTATGGCTGACTTGGTAACCCCAAATGTGAAGGAAGCATCTGCCTTACTTGGTGGTATGCCACTTAAAACAATTTCTGACATGCGTCATGCTGCAACATTAATCCATCAGATGGGGTCGAAGTAAGTTTTGAAATTGCAATTTGCTGTTTACCTCTAGCCACAGAGACTGACCTAGCACTTGTTTTTCCTCATTGTTGCTTGCCTCTTACCAATAATTTTATACCATTCTATTTAGGATGCATTCAAAATTGTTAGTATGGTTTCTGCAAGAATTTTGTTGAGAATATTATCAGGGTTATGTCAGAAATTCTATTAGGACACTAAGGGTATATTGTTGGTCATTAGCTAAGGAATTTGTTAGTAAGGGGGGTTATAAATAAACCATCATGGATGGTTTGTTATTAAAGCTCTGGAGGAATAGGTTCAAACTCCATGGGTATCAAAAGAAGGAAGGAGAAAGAGATAGGTGGCTATAAAACGAGTGAGGGAGAGAGGTGGAGGTAAGCAATTGTTTAGTTGTTTAGGCTTGGATTTACTCAAAGATAAGCATCTAAGGAGCCGTTTGGTTTAAGGTTTTATAGATGCCTAGGAATAAAGGATGCCTGAGAATACTATGCCTGGAAATAAGATTGCTGGGAACTGTGTTTGGTTGTGCATGGGAATGCCATCAGGATTTTATGAGAACGAATTATGTCTGTATTTATTTTTTAGAATTAATCAGGTAAACTTTATGTAATTTTAATAAATTAATCAATAAAATAATCAAAATTAATTCAAACTATTAATAAAAGTTAATGAATTAATAATCATGAAATAATTTTAATTGGATAAACTATGTTAATTTAACTAATTAAAATTATTTTATTAATTTTTTTTAATAAATATCCTTAACTACAATAATTTAATATATTTGTATCCATAAAAATTAATTAGATAAATATTTTAATAATAATATGATTATTTATTTATTATAAAGAAATCCAAAAAAATAATTAATTGATTATAAATAATCAATAAGTAATTAATTATAATTATTGAAAAATGAATTTTTATACTAAAATCATTTAAATAATTTTAATTCAATAATTAAATATATTTTCGATAATGTTAACTAATTGATTATAATTTTAGTAAATATGTAATTACATCCCTTAATAAACTTGGACTAAGTATTTGTTTAAAAAAAACTTGAAATTCTTGACAAATTATTATTTAATATAATTAAATAATTTATTATATTATTTTTTAATTTAGTATCCATTAGTTAATAAAATTGCATATAAAAATAAAAAAGAAGAGATGAGAAAAGAGAAAACCCATGCTTTTGCAAGGGTATAGAAAACCCTTGCTTATGGGCATAAATGATGTATGGGAATCCGTTAATTCCTGGGCCCATCACAGAAACCTTGAAACAATCAAGGGTATCAGAAACCCTTGCACCAAACAGCTCCTAAGTTCCTCTAATTACTTAGACATTTTCTTTTTGTAATTTCTTATTGGGCAAGTTTTTTATTTTTATTTATTTATTTATTATTATTATTATTTTAATAAGAAACCATGCATTCATTAAAGAAAATGAAAGGTTATTCGGAAAGTTATTAACTATTAAGATAATCTGGATTTCAACTCTCTACCCAATTAAAATCGACCTTGTACATTTTGATGCTTCAGCTTCATACTGCCATTTTTAAGTTGTAGAAGGTTTTGTTGTGAAGGCTGGACCGCAATATCCACAGTTGACATTTGATTGAATGGTTTTATATTTATCAAACCATTCCTGACTGATGTGATAGAACCCAGACAAAACCAAAATGATATTGAATTATAAAAGATAAAAGAATTATATGATAACCAAGTAAGTTGAGTTAATTGGACCATTCCTCTCTTAATGTCACTTGAGTCTAAACCAACTGAATCAATGCCTACCTTCAACATTTTTCACCCTCTATTTATAACCAAACCCTCCTAACGAGCTCCTTAGCTAAATAATATTCCCACTTCTATCCCAATAGCATTCCTATTATGTTGCTGAAGAGTGTATATTTGTTTTCAGAAATGTACTTGTCAAAGGTGGGGATCTTCACGATTCATTGGATGCCGTTGATATATTCTTTGATGGTGAGTATAGATTGCAAAATTTTGACCATTTGATATTTTTTTTATGAAGGAAACAGATCCATTGTACCCAAATTTTGAACTAATTTTCACAGGCAAGGACTTACATGAGCTACGTTCTCCACGCATAAAGACTCTCAACACTCATGGTACTGGATGCAGCTTAGCATCATGCATTGCAGCTGAACTCGCAAAAGGGTCTTCAATGTTCTCAGCTGTCAAGGTAATCTAGTTATCAGGGACCTGATCTTTTAATCTCACGAATAATGACTTCAGCAATAAGAAGATTAAGGAAGTTTAAATGGCTCGGAATAGAACTTGCTAGTGAATAGTTAAATTTATAATAAAATAAAATCAAATTGTGAACCACTTTCAATTTATTGTAGGATAATAATTGCTCAAAATAATGCTATATTTCACCCGTTTCTCTTTGGCTTGTTATTTGTATATCCTTGTATTCTTTCATCTATTCAATGAAATTGTGGTTTCTTACCACCCCCACTATATTCACTATGTATGTAGGTTCTCTTACCTTGGTAAAGACTTCAGATGTTTGGAGATACCTTCCAAGGTCTTGAGTTCAAGCTTGCAAGTTGAAAGCTTAATAACTTTGGGGTGTTTAGTTAGTGATCTAAAATTAGGAATTTGAAAATTGGGATTTAAGGGAATAGAGTTGTATTTCATATTTTCATTGTTTTTAATGTGTTTGGTAGTGGATTTAGAATATGAAAACACTGAAAATATGTAATTATTGTTTTCAAAATCCAATTTTTGGGTAGTAGAATTCAAATACAACTAGAAATAAACCCACCAAAAAGAAAAGTCGCTAAATTTATTTCACTCCGTGATTCTAAAAACTAGAAATTGAATTTAGATAAGACATCAAATAGGCCATTAAAACTTTAGTTATTTGTCAAAGCTAGCCTTAGGATGGGAAGCCGCTCAGATATTAGTTAGGGATGGGCTATGAGCATTATGTAAGGAATGCTGGCCCCGACACCTTAGTTATCAAAAAGGAAAATGGAAAATAAAAATCCCTTCACTTCGAATTAAAATCTTGTTTTGGTGGTGTTTTTCAATGCGCTAGCCAAAAGAAAAAATAATAGTTACATTATGGTTGTCCCTTAATCCAATTTGGAGTTTTGAATTCTTTGATTATTTTTATCTACTTTCTTTTTGGATGAAAATTTTTCACTCCATGTCATTTAACTTGATCAATTCAAATCACAATAGGCTTGGTTAGACTTGACTGTCCACAACTAAGGTTTTATTTATCTGGGGATCTTTACAGACAAGCAAACAGTTTATTGAAAGAGCCTTGAATTACAGCAAGGACATCAGCATTGGAAATGGACCTCAAGGCCCATTCGATCATCTATGTTGCCTTAAGGGTCGCGACCGAAGTTCCTATAGACAGGGGGGTTTTAGTCCAGCTGACTTATTCTTGTATGCAGTTACGGACTCGGGTATGAATAAGCGTTGGGACCGTCCTATCACAGATGCTGTTAAAGCTGCAGTAGAAGGAGGTGCTACTATTGTTCAAATAAGGTTTGTCAAATGTCTTCCATCAATCAATCTGTTATTTAACATTTTGTTCGTTAAAAATGTATTCACAAGTTGATATATATTAAAGTCATTACCTCAAATATACGGAATTCTTGTTGCATTTTAACGTCCTCTTTAAGACCTCTTCAGTTTAAAGGTTAATTGGCATTAGACCCACGAAACCTAGGCTAAATGCTCCTTAGGTGATCGATAGTGAATTAGTTCTTGCAGGTGAAAGGAACCCGTCAGTAAGATTTGAATATCTTAATTAACTCGTCGCATTCTTTTTGAGAGAAATTAAATACTTAGCATTGTTTTAGAACTTGTATTCGGTTTTTAATTTTCTTCCAATGATTAGTGTTCCCTTATTTCTATGAACAGGTATTTAATTTTCTTCCAATGATTAGTGTTCCCTTATTTCTATGAACAGGGAAAAAGAAGCCAAAACTCGCGATTTCTTGGAAGCAGCCAAGTCATGCCTGAATATTTGTCATGCGCATGGAGTTCCATTGTTGATTAATGATCGTATTGACGTTGCACTTGCATGCGATGCTGATGGTGTACACATTGGTCAATCCGACATTCCTACTCACGTTGTTCGCAGCCTTCTCGGCCCTGATAAGATCATCGGTGTCTCGTGCAAGACACCAGAGCAAGCAGAACAGGCATGGCTTGATGGTGCTGATTACATAGGGTGTGGTGGAGTTTATCCCACAAATACAAAAGCAAACAATCTGACTATTGGGCTTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAGTTGCCTGTGGTTGCAATTGGTGGGATTAACCACACAAATGCAGCAGCTGTGATGGAAATTGGTATTCCAAATCTTAAAGGTGTTGCAGTTGTGTCAGCTCTTTTTGATCAGCAATGTGTTTTAGAGGAGACCTTAAAGTTACATGCAACTTTAGTGGAGGCTACAACATTAGCTGTGAAATGAATGAGAGACCATATTGTTATGATGGCTCCTTTGGTCTTTGGACTTTGATGAAATCAATCAAATGTATGATTGTAATGCTTAGAATCTTTTTGGAATAAAAATAATTGTTGATAATATGATTATTAAAGTAACAAAATGTGTGTATATTTAGTCATAGTTAAGTAAAAGAACAATTACTTAGTCATAATTAGATAAAAG

mRNA sequence

ATGAGTAAGATTGGAAGGCTTTTACGAAAGTCCTCTGCTTGGAAAAGGGACATCTTGTCCCTTTGTCCGTCAGCTGCTTTGGAAGCTTTCCTGAATGGAATTTCTGGCATGGAAGAACTTCTACCTATGGCTGACTTGGTAACCCCAAATGTGAAGGAAGCATCTGCCTTACTTGGTGGTATGCCACTTAAAACAATTTCTGACATGCGTCATGCTGCAACATTAATCCATCAGATGGGGTCGAAAAATGTACTTGTCAAAGGTGGGGATCTTCACGATTCATTGGATGCCGTTGATATATTCTTTGATGGCAAGGACTTACATGAGCTACGTTCTCCACGCATAAAGACTCTCAACACTCATGGTACTGGATGCAGCTTAGCATCATGCATTGCAGCTGAACTCGCAAAAGGGTCTTCAATGTTCTCAGCTGTCAAGACAAGCAAACAGTTTATTGAAAGAGCCTTGAATTACAGCAAGGACATCAGCATTGGAAATGGACCTCAAGGCCCATTCGATCATCTATGTTGCCTTAAGGGTCGCGACCGAAGTTCCTATAGACAGGGGGGTTTTAGTCCAGCTGACTTATTCTTGTATGCAGTTACGGACTCGGGTATGAATAAGCGTTGGGACCGTCCTATCACAGATGCTGTTAAAGCTGCAGTAGAAGGAGGTGCTACTATTGTTCAAATAAGGGAAAAAGAAGCCAAAACTCGCGATTTCTTGGAAGCAGCCAAGTCATGCCTGAATATTTGTCATGCGCATGGAGTTCCATTGTTGATTAATGATCGTATTGACGTTGCACTTGCATGCGATGCTGATGGTGTACACATTGGTCAATCCGACATTCCTACTCACGTTGTTCGCAGCCTTCTCGGCCCTGATAAGATCATCGGTGTCTCGTGCAAGACACCAGAGCAAGCAGAACAGGCATGGCTTGATGGTGCTGATTACATAGGGTGTGGTGGAGTTTATCCCACAAATACAAAAGCAAACAATCTGACTATTGGGCTTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAGTTGCCTGTGGTTGCAATTGGTGGGATTAACCACACAAATGCAGCAGCTGTGATGGAAATTGGTATTCCAAATCTTAAAGGTGTTGCAGTTGTGTCAGCTCTTTTTGATCAGCAATGTGTTTTAGAGGAGACCTTAAAGTTACATGCAACTTTAGTGGAGGCTACAACATTAGCTGTGAAATGA

Coding sequence (CDS)

ATGAGTAAGATTGGAAGGCTTTTACGAAAGTCCTCTGCTTGGAAAAGGGACATCTTGTCCCTTTGTCCGTCAGCTGCTTTGGAAGCTTTCCTGAATGGAATTTCTGGCATGGAAGAACTTCTACCTATGGCTGACTTGGTAACCCCAAATGTGAAGGAAGCATCTGCCTTACTTGGTGGTATGCCACTTAAAACAATTTCTGACATGCGTCATGCTGCAACATTAATCCATCAGATGGGGTCGAAAAATGTACTTGTCAAAGGTGGGGATCTTCACGATTCATTGGATGCCGTTGATATATTCTTTGATGGCAAGGACTTACATGAGCTACGTTCTCCACGCATAAAGACTCTCAACACTCATGGTACTGGATGCAGCTTAGCATCATGCATTGCAGCTGAACTCGCAAAAGGGTCTTCAATGTTCTCAGCTGTCAAGACAAGCAAACAGTTTATTGAAAGAGCCTTGAATTACAGCAAGGACATCAGCATTGGAAATGGACCTCAAGGCCCATTCGATCATCTATGTTGCCTTAAGGGTCGCGACCGAAGTTCCTATAGACAGGGGGGTTTTAGTCCAGCTGACTTATTCTTGTATGCAGTTACGGACTCGGGTATGAATAAGCGTTGGGACCGTCCTATCACAGATGCTGTTAAAGCTGCAGTAGAAGGAGGTGCTACTATTGTTCAAATAAGGGAAAAAGAAGCCAAAACTCGCGATTTCTTGGAAGCAGCCAAGTCATGCCTGAATATTTGTCATGCGCATGGAGTTCCATTGTTGATTAATGATCGTATTGACGTTGCACTTGCATGCGATGCTGATGGTGTACACATTGGTCAATCCGACATTCCTACTCACGTTGTTCGCAGCCTTCTCGGCCCTGATAAGATCATCGGTGTCTCGTGCAAGACACCAGAGCAAGCAGAACAGGCATGGCTTGATGGTGCTGATTACATAGGGTGTGGTGGAGTTTATCCCACAAATACAAAAGCAAACAATCTGACTATTGGGCTTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAGTTGCCTGTGGTTGCAATTGGTGGGATTAACCACACAAATGCAGCAGCTGTGATGGAAATTGGTATTCCAAATCTTAAAGGTGTTGCAGTTGTGTCAGCTCTTTTTGATCAGCAATGTGTTTTAGAGGAGACCTTAAAGTTACATGCAACTTTAGTGGAGGCTACAACATTAGCTGTGAAATGA

Protein sequence

MSKIGRLLRKSSAWKRDILSLCPSAALEAFLNGISGMEELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDAVDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALNYSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDAVKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVHIGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIGLDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLHATLVEATTLAVK

Homology

[Close]

BLAST of Spg035332 vs. NCBI nr
Match: XP_038876926.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Benincasa hispida])

HSP 1 Score: 686.0 bits (1769), Expect = 1.9e-193
Identity = 337/368 (91.58%), Postives = 354/368 (96.20%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           +ELLPMADLVTPN+KEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDL DSLDA
Sbjct: 165 KELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDA 224

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VDIFFDGKDLHELRS RIKT NTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERAL 
Sbjct: 225 VDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALK 284

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDI IGNGPQGPFDHLC LK R++SSYRQG F+PADLFLYAVTDSGMNKRWDR I+DA
Sbjct: 285 YSKDIGIGNGPQGPFDHLCRLKSREQSSYRQGCFNPADLFLYAVTDSGMNKRWDRSISDA 344

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           VKAAVEGGATI+QIREKEAKTRDFLEAAKSC+ ICHAHGVPLLINDRID+ALACDADGVH
Sbjct: 345 VKAAVEGGATIIQIREKEAKTRDFLEAAKSCIKICHAHGVPLLINDRIDIALACDADGVH 404

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           +GQSDIP H VR LLGP+KIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLT+G
Sbjct: 405 VGQSDIPAHEVRRLLGPNKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVG 464

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           ++GLKRVCLASKLPVVAIGGINH+NAAAVMEIG+PNLKGVAVVSALFD+QCVLEE LKLH
Sbjct: 465 IEGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEEALKLH 524

Query: 398 ATLVEATT 406
           ATLVEATT
Sbjct: 525 ATLVEATT 532

[Close]

BLAST of Spg035332 vs. NCBI nr
Match: XP_022957338.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 682.6 bits (1760), Expect = 2.2e-192
Identity = 337/370 (91.08%), Postives = 352/370 (95.14%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           +ELLPMADLVTPN+KEASALLGGMPL TISDMRHAATLIHQMGSKNVLVKGGDL DSLDA
Sbjct: 145 DELLPMADLVTPNLKEASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDA 204

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VDIFFDGKDLHELRS RI T NTHGTGCSLASCIAAELAKGSSMFSAVK SKQFIERAL+
Sbjct: 205 VDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALS 264

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDISIGNGPQGPFDHLC LK R++SSYRQG F+ +DLFLYAVTDSGMNKRWDR ITDA
Sbjct: 265 YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDA 324

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           VKAAVEGGATI+QIREK+AKTRDFLEAAKSC+ ICH HGVPLLINDRIDVALAC ADGVH
Sbjct: 325 VKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVH 384

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           IGQSDIP H  RSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLT+G
Sbjct: 385 IGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVG 444

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           LDGLKRVCLASKLPVVAIGGINH+NAAAVMEIG+PNLKGVAVVSALFD+QCVLEETLKLH
Sbjct: 445 LDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLH 504

Query: 398 ATLVEATTLA 408
           ATLVEAT L+
Sbjct: 505 ATLVEATALS 514

[Close]

BLAST of Spg035332 vs. NCBI nr
Match: XP_022957337.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 682.6 bits (1760), Expect = 2.2e-192
Identity = 337/370 (91.08%), Postives = 352/370 (95.14%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           +ELLPMADLVTPN+KEASALLGGMPL TISDMRHAATLIHQMGSKNVLVKGGDL DSLDA
Sbjct: 165 DELLPMADLVTPNLKEASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDA 224

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VDIFFDGKDLHELRS RI T NTHGTGCSLASCIAAELAKGSSMFSAVK SKQFIERAL+
Sbjct: 225 VDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALS 284

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDISIGNGPQGPFDHLC LK R++SSYRQG F+ +DLFLYAVTDSGMNKRWDR ITDA
Sbjct: 285 YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDA 344

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           VKAAVEGGATI+QIREK+AKTRDFLEAAKSC+ ICH HGVPLLINDRIDVALAC ADGVH
Sbjct: 345 VKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVH 404

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           IGQSDIP H  RSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLT+G
Sbjct: 405 IGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVG 464

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           LDGLKRVCLASKLPVVAIGGINH+NAAAVMEIG+PNLKGVAVVSALFD+QCVLEETLKLH
Sbjct: 465 LDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLH 524

Query: 398 ATLVEATTLA 408
           ATLVEAT L+
Sbjct: 525 ATLVEATALS 534

[Close]

BLAST of Spg035332 vs. NCBI nr
Match: XP_022997729.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 681.8 bits (1758), Expect = 3.7e-192
Identity = 336/370 (90.81%), Postives = 351/370 (94.86%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           +ELLPM DLVTPN+KEASALLGGMPL TISDMRHAATLIHQMGSKNVLVKGGDL DSLDA
Sbjct: 165 DELLPMVDLVTPNLKEASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDA 224

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VDIFFDGKDLHELRS RI T NTHGTGCSLASCIAAELAKGSSMFSAVK SKQFIERALN
Sbjct: 225 VDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALN 284

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDISIGNGPQGPFDHLC LK R++SSYRQG F+ +DLFLYAVTDSGMNKRWDR ITDA
Sbjct: 285 YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDA 344

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           VKAAVEGGATI+QIREK+AKTRDFLEAAKSC+ ICH HGVPLLINDRIDVALAC ADGVH
Sbjct: 345 VKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVH 404

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           IGQSDIP H  RSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLT+G
Sbjct: 405 IGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVG 464

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           LDGLK+VCLASKLPVVAIGGINH+NAAAVMEIG+PNLKGVAVVSALFD+QCVLEETLKLH
Sbjct: 465 LDGLKKVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLH 524

Query: 398 ATLVEATTLA 408
           ATLVEAT L+
Sbjct: 525 ATLVEATALS 534

[Close]

BLAST of Spg035332 vs. NCBI nr
Match: XP_022997737.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 681.8 bits (1758), Expect = 3.7e-192
Identity = 336/370 (90.81%), Postives = 351/370 (94.86%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           +ELLPM DLVTPN+KEASALLGGMPL TISDMRHAATLIHQMGSKNVLVKGGDL DSLDA
Sbjct: 145 DELLPMVDLVTPNLKEASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDA 204

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VDIFFDGKDLHELRS RI T NTHGTGCSLASCIAAELAKGSSMFSAVK SKQFIERALN
Sbjct: 205 VDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALN 264

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDISIGNGPQGPFDHLC LK R++SSYRQG F+ +DLFLYAVTDSGMNKRWDR ITDA
Sbjct: 265 YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDA 324

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           VKAAVEGGATI+QIREK+AKTRDFLEAAKSC+ ICH HGVPLLINDRIDVALAC ADGVH
Sbjct: 325 VKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVH 384

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           IGQSDIP H  RSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLT+G
Sbjct: 385 IGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVG 444

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           LDGLK+VCLASKLPVVAIGGINH+NAAAVMEIG+PNLKGVAVVSALFD+QCVLEETLKLH
Sbjct: 445 LDGLKKVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLH 504

Query: 398 ATLVEATTLA 408
           ATLVEAT L+
Sbjct: 505 ATLVEATALS 514

[Close]

BLAST of Spg035332 vs. ExPASy Swiss-Prot
Match: Q5M731 (Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TH1 PE=1 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 1.7e-152
Identity = 265/366 (72.40%), Postives = 311/366 (84.97%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           E LLP+AD++TPNVKEASALL G  ++T+++MR AA  +H+MG + VLVKGGDL DS D+
Sbjct: 154 ERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLPDSSDS 213

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VD++FDGK+ HELRSPRI T NTHGTGC+LASCIAAELAKGSSM SAVK +K+F++ AL+
Sbjct: 214 VDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFVDNALD 273

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDI IG+G QGPFDH   LK +D  S R   F+P DLFLYAVTDS MNK+W+R I DA
Sbjct: 274 YSKDIVIGSGMQGPFDHFFGLK-KDPQSSRCSIFNPDDLFLYAVTDSRMNKKWNRSIVDA 333

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           +KAA+EGGATI+Q+REKEA+TR+FLE AK+C++IC +HGV LLINDRID+ALACDADGVH
Sbjct: 334 LKAAIEGGATIIQLREKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACDADGVH 393

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           +GQSD+P  +VRSLLGPDKIIGVSCKTPEQA QAW DGADYIG GGV+PTNTKANN TIG
Sbjct: 394 VGQSDMPVDLVRSLLGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKANNRTIG 453

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           LDGLK VC ASKLPVVAIGGI  +NA +VM+I  PNLKGVAVVSALFDQ CVL +  KLH
Sbjct: 454 LDGLKEVCEASKLPVVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQAKKLH 513

Query: 398 ATLVEA 404
            TL E+
Sbjct: 514 KTLKES 518

[Close]

BLAST of Spg035332 vs. ExPASy Swiss-Prot
Match: O48881 (Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus OX=3708 GN=BTH1 PE=1 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 5.0e-152
Identity = 264/366 (72.13%), Postives = 309/366 (84.43%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           E LLP+AD++TPNVKEASALLGG+ ++T+++MR AA  +HQMG + VLVKGGDL DS D+
Sbjct: 156 ERLLPLADIITPNVKEASALLGGVRIQTVAEMRSAAKSLHQMGPRFVLVKGGDLPDSSDS 215

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VD++FDG + HEL SPRI T NTHGTGC+LASCIAAELAKGS+M SAVK +K+F++ ALN
Sbjct: 216 VDVYFDGNEFHELHSPRIATRNTHGTGCTLASCIAAELAKGSNMLSAVKVAKRFVDSALN 275

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDI IG+G QGPFDH   LK  D  SYRQ  F P DLFLYAVTDS MNK+W+R I DA
Sbjct: 276 YSKDIVIGSGMQGPFDHFLSLK--DPQSYRQSTFKPDDLFLYAVTDSRMNKKWNRSIVDA 335

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           VKAA+EGGATI+Q+REKEA+TR+FLE AKSC++IC ++GV LLINDR D+A+A DADGVH
Sbjct: 336 VKAAIEGGATIIQLREKEAETREFLEEAKSCVDICRSNGVCLLINDRFDIAIALDADGVH 395

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           +GQSD+P  +VRSLLGPDKIIGVSCKT EQA QAW DGADYIG GGV+PTNTKANN TIG
Sbjct: 396 VGQSDMPVDLVRSLLGPDKIIGVSCKTQEQAHQAWKDGADYIGSGGVFPTNTKANNRTIG 455

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           LDGL+ VC ASKLPVVAIGGI  +NA +VM IG PNLKGVAVVSALFDQ+CVL +  KLH
Sbjct: 456 LDGLREVCKASKLPVVAIGGIGISNAESVMRIGEPNLKGVAVVSALFDQECVLTQAKKLH 515

Query: 398 ATLVEA 404
            TL E+
Sbjct: 516 KTLTES 519

[Close]

BLAST of Spg035332 vs. ExPASy Swiss-Prot
Match: Q2QWK9 (Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0192500 PE=2 SV=2)

HSP 1 Score: 508.4 bits (1308), Expect = 7.3e-143
Identity = 246/367 (67.03%), Postives = 302/367 (82.29%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           +EL  MAD+VTPNVKEAS LLGG+ L+T+SDMR+AA  I++ G K+VLVKGGD+ +S DA
Sbjct: 180 DELFAMADIVTPNVKEASRLLGGVSLRTVSDMRNAAESIYKFGPKHVLVKGGDMLESSDA 239

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
            D+FFDGK+  EL + RIKT NTHGTGC+LASCIA+ELAKG++M  AV+ +K F+E AL+
Sbjct: 240 TDVFFDGKEFIELHAHRIKTHNTHGTGCTLASCIASELAKGATMLHAVQVAKNFVESALH 299

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           +SKD+ +GNGPQGPFDHL  LK    +   Q  F P  LFLYAVTDSGMNK+W R I +A
Sbjct: 300 HSKDLVVGNGPQGPFDHLFKLKCPPYNVGSQPSFKPDQLFLYAVTDSGMNKKWGRSIKEA 359

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           V+AA+EGGATIVQ+REK+++TR+FLEAAK+C+ IC + GVPLLINDR+D+ALAC+ADGVH
Sbjct: 360 VQAAIEGGATIVQLREKDSETREFLEAAKACMEICKSSGVPLLINDRVDIALACNADGVH 419

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           +GQ D+  H VR LLGP KIIGVSCKTP QA+QAW DGADYIGCGGV+PT+TKANN T+G
Sbjct: 420 VGQLDMSAHEVRELLGPGKIIGVSCKTPAQAQQAWNDGADYIGCGGVFPTSTKANNPTLG 479

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
            DGLK VCLASKLPVVAIGGIN +NA +VME+G+PNLKGVAVVSALFD+  V+ ET  + 
Sbjct: 480 FDGLKTVCLASKLPVVAIGGINASNAGSVMELGLPNLKGVAVVSALFDRPSVVAETRNMK 539

Query: 398 ATLVEAT 405
           + L   +
Sbjct: 540 SILTNTS 546

[Close]

BLAST of Spg035332 vs. ExPASy Swiss-Prot
Match: A8MK92 (Thiamine-phosphate synthase OS=Alkaliphilus oremlandii (strain OhILAs) OX=350688 GN=thiE PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 5.0e-43
Identity = 93/206 (45.15%), Postives = 135/206 (65.53%), Query Frame = 0


Query: 195 DLFLYAVTDSGMNKRWDRPITDAVKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHA 254
           D  LY V+D  + K   R    +++ A+ GGATIVQ+REKEA + +F + A     +   
Sbjct: 8   DYGLYLVSDRDVLK--GRDFIKSLEEAILGGATIVQLREKEASSLEFYQLALKAKALTEK 67

Query: 255 HGVPLLINDRIDVALACDADGVHIGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLD 314
           + VPL+INDR+D+ALA DADGVH+GQSD+P H+VRS++G +KI+GVS  T E++++A  D
Sbjct: 68  YNVPLIINDRVDIALAVDADGVHVGQSDLPAHIVRSMIGQNKILGVSTATLEESKKAAED 127

Query: 315 GADYIGCGGVYPTNTKANNLTIGLDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNL 374
           GADYIG G ++PT TK +   + LD L+ +     +PVV IGGI   N   +ME+GI   
Sbjct: 128 GADYIGVGALFPTGTKTDANPVTLDQLRYIKENMDIPVVGIGGICEDNIKTIMEVGI--- 187

Query: 375 KGVAVVSALFDQQCVLEETLKLHATL 401
            GVA+VSA+  ++ + E    L A++
Sbjct: 188 DGVAIVSAILGKENIKEAAESLKASI 208

[Close]

BLAST of Spg035332 vs. ExPASy Swiss-Prot
Match: Q97LQ9 (Thiamine-phosphate synthase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=thiE PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.8e-41
Identity = 90/198 (45.45%), Postives = 129/198 (65.15%), Query Frame = 0


Query: 195 DLFLYAVTDSGMNKRWDRPITDAVKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHA 254
           D  LY VTD  + K  +R +  +++ A++GG T+VQ+REKE  T DF E+A     I   
Sbjct: 5   DYKLYLVTDRKVLK--ERDLYKSIEEAIKGGVTLVQLREKEMSTLDFYESALKLKKITET 64

Query: 255 HGVPLLINDRIDVALACDADGVHIGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLD 314
           + +PL+INDRID+ALA +ADGVHIGQSD+P    R LLG DKIIGVS  + E+A +A  +
Sbjct: 65  YKIPLIINDRIDIALAINADGVHIGQSDMPLIKARELLGKDKIIGVSAHSIEEALEAERN 124

Query: 315 GADYIGCGGVYPTNTKANNLTIGLDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNL 374
           GA Y+G G +Y T+TK +   + L+ LK +  + K+PVV IGGIN  NA  V+E G+   
Sbjct: 125 GATYLGVGAIYNTSTKGDAQAVSLEELKNIKNSVKIPVVGIGGINEENANKVIETGV--- 184

Query: 375 KGVAVVSALFDQQCVLEE 393
            G++V+S +   Q + ++
Sbjct: 185 DGISVISGILSAQKIKDK 197

[Close]

BLAST of Spg035332 vs. ExPASy TrEMBL
Match: A0A6J1GZX4 (Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC111458768 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.0e-192
Identity = 337/370 (91.08%), Postives = 352/370 (95.14%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           +ELLPMADLVTPN+KEASALLGGMPL TISDMRHAATLIHQMGSKNVLVKGGDL DSLDA
Sbjct: 145 DELLPMADLVTPNLKEASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDA 204

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VDIFFDGKDLHELRS RI T NTHGTGCSLASCIAAELAKGSSMFSAVK SKQFIERAL+
Sbjct: 205 VDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALS 264

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDISIGNGPQGPFDHLC LK R++SSYRQG F+ +DLFLYAVTDSGMNKRWDR ITDA
Sbjct: 265 YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDA 324

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           VKAAVEGGATI+QIREK+AKTRDFLEAAKSC+ ICH HGVPLLINDRIDVALAC ADGVH
Sbjct: 325 VKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVH 384

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           IGQSDIP H  RSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLT+G
Sbjct: 385 IGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVG 444

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           LDGLKRVCLASKLPVVAIGGINH+NAAAVMEIG+PNLKGVAVVSALFD+QCVLEETLKLH
Sbjct: 445 LDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLH 504

Query: 398 ATLVEATTLA 408
           ATLVEAT L+
Sbjct: 505 ATLVEATALS 514

[Close]

BLAST of Spg035332 vs. ExPASy TrEMBL
Match: A0A6J1GYV1 (Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC111458768 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.0e-192
Identity = 337/370 (91.08%), Postives = 352/370 (95.14%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           +ELLPMADLVTPN+KEASALLGGMPL TISDMRHAATLIHQMGSKNVLVKGGDL DSLDA
Sbjct: 165 DELLPMADLVTPNLKEASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDA 224

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VDIFFDGKDLHELRS RI T NTHGTGCSLASCIAAELAKGSSMFSAVK SKQFIERAL+
Sbjct: 225 VDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALS 284

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDISIGNGPQGPFDHLC LK R++SSYRQG F+ +DLFLYAVTDSGMNKRWDR ITDA
Sbjct: 285 YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDA 344

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           VKAAVEGGATI+QIREK+AKTRDFLEAAKSC+ ICH HGVPLLINDRIDVALAC ADGVH
Sbjct: 345 VKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVH 404

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           IGQSDIP H  RSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLT+G
Sbjct: 405 IGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVG 464

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           LDGLKRVCLASKLPVVAIGGINH+NAAAVMEIG+PNLKGVAVVSALFD+QCVLEETLKLH
Sbjct: 465 LDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLH 524

Query: 398 ATLVEATTLA 408
           ATLVEAT L+
Sbjct: 525 ATLVEATALS 534

[Close]

BLAST of Spg035332 vs. ExPASy TrEMBL
Match: A0A6J1KCE1 (Thiamine-phosphate pyrophosphorylase OS=Cucurbita maxima OX=3661 GN=LOC111492599 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.8e-192
Identity = 336/370 (90.81%), Postives = 351/370 (94.86%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           +ELLPM DLVTPN+KEASALLGGMPL TISDMRHAATLIHQMGSKNVLVKGGDL DSLDA
Sbjct: 165 DELLPMVDLVTPNLKEASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDA 224

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VDIFFDGKDLHELRS RI T NTHGTGCSLASCIAAELAKGSSMFSAVK SKQFIERALN
Sbjct: 225 VDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALN 284

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDISIGNGPQGPFDHLC LK R++SSYRQG F+ +DLFLYAVTDSGMNKRWDR ITDA
Sbjct: 285 YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDA 344

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           VKAAVEGGATI+QIREK+AKTRDFLEAAKSC+ ICH HGVPLLINDRIDVALAC ADGVH
Sbjct: 345 VKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVH 404

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           IGQSDIP H  RSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLT+G
Sbjct: 405 IGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVG 464

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           LDGLK+VCLASKLPVVAIGGINH+NAAAVMEIG+PNLKGVAVVSALFD+QCVLEETLKLH
Sbjct: 465 LDGLKKVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLH 524

Query: 398 ATLVEATTLA 408
           ATLVEAT L+
Sbjct: 525 ATLVEATALS 534

[Close]

BLAST of Spg035332 vs. ExPASy TrEMBL
Match: A0A6J1K5X2 (Thiamine-phosphate pyrophosphorylase OS=Cucurbita maxima OX=3661 GN=LOC111492599 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.8e-192
Identity = 336/370 (90.81%), Postives = 351/370 (94.86%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           +ELLPM DLVTPN+KEASALLGGMPL TISDMRHAATLIHQMGSKNVLVKGGDL DSLDA
Sbjct: 145 DELLPMVDLVTPNLKEASALLGGMPLNTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDA 204

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VDIFFDGKDLHELRS RI T NTHGTGCSLASCIAAELAKGSSMFSAVK SKQFIERALN
Sbjct: 205 VDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSMFSAVKISKQFIERALN 264

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDISIGNGPQGPFDHLC LK R++SSYRQG F+ +DLFLYAVTDSGMNKRWDR ITDA
Sbjct: 265 YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAVTDSGMNKRWDRSITDA 324

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           VKAAVEGGATI+QIREK+AKTRDFLEAAKSC+ ICH HGVPLLINDRIDVALAC ADGVH
Sbjct: 325 VKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVH 384

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           IGQSDIP H  RSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLT+G
Sbjct: 385 IGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVG 444

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           LDGLK+VCLASKLPVVAIGGINH+NAAAVMEIG+PNLKGVAVVSALFD+QCVLEETLKLH
Sbjct: 445 LDGLKKVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLH 504

Query: 398 ATLVEATTLA 408
           ATLVEAT L+
Sbjct: 505 ATLVEATALS 514

[Close]

BLAST of Spg035332 vs. ExPASy TrEMBL
Match: A0A6J1DTL9 (Thiamine-phosphate pyrophosphorylase OS=Momordica charantia OX=3673 GN=LOC111023892 PE=3 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 1.4e-189
Identity = 336/372 (90.32%), Postives = 354/372 (95.16%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           EELLPMADLVTPN+KEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDL DSLDA
Sbjct: 179 EELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDA 238

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VDIFFDGKDLHEL S RIKT NTHGTGC+LASCIAAELAKGSSMFSAVKTSK FIERAL+
Sbjct: 239 VDIFFDGKDLHELCSSRIKTRNTHGTGCTLASCIAAELAKGSSMFSAVKTSKWFIERALD 298

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDISIGNGPQGPFDHLC LK RDRSS+ Q GF+PADLFLYAVTDSGMNKRWDR ITDA
Sbjct: 299 YSKDISIGNGPQGPFDHLCQLKTRDRSSHSQRGFNPADLFLYAVTDSGMNKRWDRSITDA 358

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           VKAAVEGGATIVQIREK+A+T +FLEAAKSCL IC AHGVPLLINDR+DVALACDADGVH
Sbjct: 359 VKAAVEGGATIVQIREKDAETCNFLEAAKSCLEICRAHGVPLLINDRVDVALACDADGVH 418

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           +GQSDIP H VRSLLGP+KIIGVSCKTPEQAE+AWLDGADYIGCGGVYPTNTKANNLT+G
Sbjct: 419 VGQSDIPAHAVRSLLGPNKIIGVSCKTPEQAERAWLDGADYIGCGGVYPTNTKANNLTVG 478

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           +DGLKRVCL SKLPVVAIGGINHTNAAAVMEIG+PNL+GVAVVSALFD+QCVLEETLKLH
Sbjct: 479 IDGLKRVCLGSKLPVVAIGGINHTNAAAVMEIGVPNLEGVAVVSALFDRQCVLEETLKLH 538

Query: 398 ATLVEATTLAVK 410
           A LVEAT+ AVK
Sbjct: 539 ARLVEATS-AVK 549

[Close]

BLAST of Spg035332 vs. TAIR 10
Match: AT1G22940.1 (thiamin biosynthesis protein, putative )

HSP 1 Score: 540.4 bits (1391), Expect = 1.2e-153
Identity = 265/366 (72.40%), Postives = 311/366 (84.97%), Query Frame = 0


Query: 38  EELLPMADLVTPNVKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLHDSLDA 97
           E LLP+AD++TPNVKEASALL G  ++T+++MR AA  +H+MG + VLVKGGDL DS D+
Sbjct: 154 ERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLPDSSDS 213

Query: 98  VDIFFDGKDLHELRSPRIKTLNTHGTGCSLASCIAAELAKGSSMFSAVKTSKQFIERALN 157
           VD++FDGK+ HELRSPRI T NTHGTGC+LASCIAAELAKGSSM SAVK +K+F++ AL+
Sbjct: 214 VDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFVDNALD 273

Query: 158 YSKDISIGNGPQGPFDHLCCLKGRDRSSYRQGGFSPADLFLYAVTDSGMNKRWDRPITDA 217
           YSKDI IG+G QGPFDH   LK +D  S R   F+P DLFLYAVTDS MNK+W+R I DA
Sbjct: 274 YSKDIVIGSGMQGPFDHFFGLK-KDPQSSRCSIFNPDDLFLYAVTDSRMNKKWNRSIVDA 333

Query: 218 VKAAVEGGATIVQIREKEAKTRDFLEAAKSCLNICHAHGVPLLINDRIDVALACDADGVH 277
           +KAA+EGGATI+Q+REKEA+TR+FLE AK+C++IC +HGV LLINDRID+ALACDADGVH
Sbjct: 334 LKAAIEGGATIIQLREKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACDADGVH 393

Query: 278 IGQSDIPTHVVRSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTIG 337
           +GQSD+P  +VRSLLGPDKIIGVSCKTPEQA QAW DGADYIG GGV+PTNTKANN TIG
Sbjct: 394 VGQSDMPVDLVRSLLGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKANNRTIG 453

Query: 338 LDGLKRVCLASKLPVVAIGGINHTNAAAVMEIGIPNLKGVAVVSALFDQQCVLEETLKLH 397
           LDGLK VC ASKLPVVAIGGI  +NA +VM+I  PNLKGVAVVSALFDQ CVL +  KLH
Sbjct: 454 LDGLKEVCEASKLPVVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQAKKLH 513

Query: 398 ATLVEA 404
            TL E+
Sbjct: 514 KTLKES 518

The following BLAST results are available for this feature:

BLAST of Spg035332 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876926.1	1.9e-193	91.58	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Benincasa hispida]	[more]
XP_022957338.1	2.2e-192	91.08	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucurbi...	[more]
XP_022957337.1	2.2e-192	91.08	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucurbi...	[more]
XP_022997729.1	3.7e-192	90.81	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucurbi...	[more]
XP_022997737.1	3.7e-192	90.81	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucurbi...	[more]

BLAST of Spg035332 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5M731	1.7e-152	72.40	Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thal...	[more]
O48881	5.0e-152	72.13	Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus ...	[more]
Q2QWK9	7.3e-143	67.03	Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativ...	[more]
A8MK92	5.0e-43	45.15	Thiamine-phosphate synthase OS=Alkaliphilus oremlandii (strain OhILAs) OX=350688...	[more]
Q97LQ9	2.8e-41	45.45	Thiamine-phosphate synthase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM...	[more]

BLAST of Spg035332 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GZX4	1.0e-192	91.08	Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC1114587...	[more]
A0A6J1GYV1	1.0e-192	91.08	Thiamine-phosphate pyrophosphorylase OS=Cucurbita moschata OX=3662 GN=LOC1114587...	[more]
A0A6J1KCE1	1.8e-192	90.81	Thiamine-phosphate pyrophosphorylase OS=Cucurbita maxima OX=3661 GN=LOC111492599...	[more]
A0A6J1K5X2	1.8e-192	90.81	Thiamine-phosphate pyrophosphorylase OS=Cucurbita maxima OX=3661 GN=LOC111492599...	[more]
A0A6J1DTL9	1.4e-189	90.32	Thiamine-phosphate pyrophosphorylase OS=Momordica charantia OX=3673 GN=LOC111023...	[more]

BLAST of Spg035332 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G22940.1	1.2e-153	72.40	thiamin biosynthesis protein, putative	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029056	Ribokinase-like	GENE3D	3.40.1190.20		coord: 21..189 e-value: 1.7E-41 score: 144.2
IPR029056	Ribokinase-like	SUPERFAMILY	53613	Ribokinase-like	coord: 37..171
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 190..403 e-value: 1.7E-72 score: 244.9
IPR022998	Thiamine phosphate synthase/TenI	PFAM	PF02581	TMP-TENI	coord: 198..383 e-value: 2.7E-63 score: 212.5
IPR022998	Thiamine phosphate synthase/TenI	CDD	cd00564	TMP_TenI	coord: 198..398 e-value: 5.94164E-83 score: 250.513
IPR034291	Thiamine phosphate synthase	TIGRFAM	TIGR00693	TIGR00693	coord: 198..387 e-value: 6.1E-62 score: 206.4
IPR034291	Thiamine phosphate synthase	HAMAP	MF_00097	TMP_synthase	coord: 195..402 score: 26.608158
IPR013749	Pyridoxamine kinase/Phosphomethylpyrimidine kinase	PFAM	PF08543	Phos_pyr_kin	coord: 38..168 e-value: 8.6E-40 score: 136.7
IPR045029	Hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	PANTHER	PTHR20858	PHOSPHOMETHYLPYRIMIDINE KINASE	coord: 37..399
IPR036206	Thiamin phosphate synthase superfamily	SUPERFAMILY	51391	Thiamin phosphate synthase	coord: 194..396

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg035332.1	Spg035332.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009228	thiamine biosynthetic process
biological_process	GO:0009229	thiamine diphosphate biosynthetic process
molecular_function	GO:0008972	phosphomethylpyrimidine kinase activity
molecular_function	GO:0004789	thiamine-phosphate diphosphorylase activity
molecular_function	GO:0003824	catalytic activity