Spg034899 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg034899
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold3: 25988680 .. 25989852 (+)
RNA-Seq Expression	Spg034899
Synteny	Spg034899

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAACACTTCTATGACTACTTCAATCCTCTTCTTCTTCTTCTATTTGTTCATTCAAACATCAACTGCAGCCAATTATAATGTGCTTAGTTTCGGTGCCAAACCCGATGGAAAAACCGACTCGACCAAACCATTTCTCAAAGCATGGACGTCAGCCTGTAGCTCCTCGACACCATCCACCATCAACGTCCCCAAAGGAAGGTTCTTGCTTACTCCCATCACATTTAGAGGTCCGTGTAAAAACAAAATCACATTTCGCTTAAATGGAACGCTTGTCGCACCTCTAGATTATCGTGCCCTTGGAAATTCTGGGTATTGGATTTTGTTCATCAAAGTTGATGGAGTTTCTGTCTTTGGTGGCAACATTGATGCAAAGGGTGCTGGGTATTGGGCTTGCAAAAAATTTGGAAGAAATTGCCCAGTTGGAGCTAGGGTGAGTAATTAACATATATACTTAGACTTTATACAATTTCTTTTTGTTTGTTTTGGATCCATTTATATGTATGTCCTAATGGTCTCTCTTCGTATTAATGAAATGTAGTCAATGACATTTAATTGGGCAAACAATATCCTGGTTAGTGGATTGACCTCGGTCAACAGCCAACAGACTCATCTTGTGATCAATGGTTGCAACAATGTATGGGTAAAAAATGTGAAGGCGATGGCTCCAGATCAAAGCCCTAATACAGATGGCATTCACGTTCAATCATCTACTGGTGTTATGATCAGTGGAAGCACCCTCCAAACAGGAGATGATTGCATATCCATTGGAGATGGAACTAAAAATTTGTTTATGAGTAACATCAAATGTGGGCCTGGACATGGTGTAAGGTAATGTGTATATAGAAAGACAGTTTACCATATTTATATATTGGTGTTTAATTTTGAGGTAAAACAATATGATCTAACTTCAATGAATGCATGCAGTATTGGTAGTTTGGGAAAAGAGTTTAATGAAGATGGAGTCGAGAATGTAACTTTGACAAATGCAGTGTTTATTGGATCTGATAATGGTGTGAGGATAAAGACATGGCCGACACCTAGCAAAGGTTTTGTGAGAAACGTTCTCTTTCAAAACATCATTATGAACAATGTTAAGAATCCCATCGTAATCGATCAAAACTATTGTCCGAACAAAGGTTGTCCTCCCAAGGTAATTATTAAAATCTAG

mRNA sequence

ATGGCTAACACTTCTATGACTACTTCAATCCTCTTCTTCTTCTTCTATTTGTTCATTCAAACATCAACTGCAGCCAATTATAATGTGCTTAGTTTCGGTGCCAAACCCGATGGAAAAACCGACTCGACCAAACCATTTCTCAAAGCATGGACGTCAGCCTGTAGCTCCTCGACACCATCCACCATCAACGTCCCCAAAGGAAGGTTCTTGCTTACTCCCATCACATTTAGAGGTCCGTGTAAAAACAAAATCACATTTCGCTTAAATGGAACGCTTGTCGCACCTCTAGATTATCGTGCCCTTGGAAATTCTGGGTATTGGATTTTGTTCATCAAAGTTGATGGAGTTTCTGTCTTTGGTGGCAACATTGATGCAAAGGGTGCTGGGTATTGGGCTTGCAAAAAATTTGGAAGAAATTGCCCAGTTGGAGCTAGGTCAATGACATTTAATTGGGCAAACAATATCCTGGTTAGTGGATTGACCTCGGTCAACAGCCAACAGACTCATCTTGTGATCAATGGTTGCAACAATGTATGGGTAAAAAATGTGAAGGCGATGGCTCCAGATCAAAGCCCTAATACAGATGGCATTCACGTTCAATCATCTACTGGTGTTATGATCAGTGGAAGCACCCTCCAAACAGGAGATGATTGCATATCCATTGGAGATGGAACTAAAAATTTGTTTATGAGTAACATCAAATGTGGGCCTGGACATGGTGTAAGTATTGGTAGTTTGGGAAAAGAGTTTAATGAAGATGGAGTCGAGAATGTAACTTTGACAAATGCAGTGTTTATTGGATCTGATAATGGTGTGAGGATAAAGACATGGCCGACACCTAGCAAAGGTTTTGTGAGAAACGTTCTCTTTCAAAACATCATTATGAACAATGTTAAGAATCCCATCGTAATCGATCAAAACTATTGTCCGAACAAAGGTTGTCCTCCCAAGGTAATTATTAAAATCTAG

Coding sequence (CDS)

ATGGCTAACACTTCTATGACTACTTCAATCCTCTTCTTCTTCTTCTATTTGTTCATTCAAACATCAACTGCAGCCAATTATAATGTGCTTAGTTTCGGTGCCAAACCCGATGGAAAAACCGACTCGACCAAACCATTTCTCAAAGCATGGACGTCAGCCTGTAGCTCCTCGACACCATCCACCATCAACGTCCCCAAAGGAAGGTTCTTGCTTACTCCCATCACATTTAGAGGTCCGTGTAAAAACAAAATCACATTTCGCTTAAATGGAACGCTTGTCGCACCTCTAGATTATCGTGCCCTTGGAAATTCTGGGTATTGGATTTTGTTCATCAAAGTTGATGGAGTTTCTGTCTTTGGTGGCAACATTGATGCAAAGGGTGCTGGGTATTGGGCTTGCAAAAAATTTGGAAGAAATTGCCCAGTTGGAGCTAGGTCAATGACATTTAATTGGGCAAACAATATCCTGGTTAGTGGATTGACCTCGGTCAACAGCCAACAGACTCATCTTGTGATCAATGGTTGCAACAATGTATGGGTAAAAAATGTGAAGGCGATGGCTCCAGATCAAAGCCCTAATACAGATGGCATTCACGTTCAATCATCTACTGGTGTTATGATCAGTGGAAGCACCCTCCAAACAGGAGATGATTGCATATCCATTGGAGATGGAACTAAAAATTTGTTTATGAGTAACATCAAATGTGGGCCTGGACATGGTGTAAGTATTGGTAGTTTGGGAAAAGAGTTTAATGAAGATGGAGTCGAGAATGTAACTTTGACAAATGCAGTGTTTATTGGATCTGATAATGGTGTGAGGATAAAGACATGGCCGACACCTAGCAAAGGTTTTGTGAGAAACGTTCTCTTTCAAAACATCATTATGAACAATGTTAAGAATCCCATCGTAATCGATCAAAACTATTGTCCGAACAAAGGTTGTCCTCCCAAGGTAATTATTAAAATCTAG

Protein sequence

MANTSMTTSILFFFFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPSTINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFGGNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKNPIVIDQNYCPNKGCPPKVIIKI

Homology

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BLAST of Spg034899 vs. NCBI nr
Match: XP_038888873.1 (polygalacturonase-like [Benincasa hispida] >XP_038888876.1 polygalacturonase-like [Benincasa hispida])

HSP 1 Score: 560.5 bits (1443), Expect = 9.7e-156
Identity = 274/317 (86.44%), Postives = 289/317 (91.17%), Query Frame = 0


Query: 1   MANTSMTTSILFFFF-YLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTP 60
           MANTS  T   FFFF YL IQTS+A ++NV+SFGAK DGKTDSTK FLKAWTSACSSSTP
Sbjct: 1   MANTSSITFFFFFFFLYLLIQTSSAGSFNVISFGAKGDGKTDSTKSFLKAWTSACSSSTP 60

Query: 61  STINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVF 120
           STINVPKGRFLLTPITFRGPC N ITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVS F
Sbjct: 61  STINVPKGRFLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFF 120

Query: 121 GGNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVW 180
           GGNIDAKGAGYWACKK G+ CPVGARS+TFNWANNI VSG+TS+NSQQTHLVIN C NV 
Sbjct: 121 GGNIDAKGAGYWACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVM 180

Query: 181 VKNVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGH 240
           VKNVK MAPDQSPNTDGIHVQSS  V I+GSTLQTGDDCISIGDGTK+L MS++KCGPGH
Sbjct: 181 VKNVKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGH 240

Query: 241 GVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVK 300
           GVSIGSLGKEFNEDGVENVTLTNA+FIGSDNGVRIKTWPT S GFV+NVLFQNIIM NVK
Sbjct: 241 GVSIGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVK 300

Query: 301 NPIVIDQNYCP-NKGCP 316
           NPI+IDQNYCP NKGCP
Sbjct: 301 NPILIDQNYCPNNKGCP 317

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BLAST of Spg034899 vs. NCBI nr
Match: XP_022156860.1 (polygalacturonase-like [Momordica charantia])

HSP 1 Score: 552.7 bits (1423), Expect = 2.0e-153
Identity = 267/305 (87.54%), Postives = 282/305 (92.46%), Query Frame = 0


Query: 12  FFFFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPSTINVPKGRFLL 71
           FFFF+LF Q S+A +YNVLSFGAKPDGKTDST+PFLKAW SAC+S T STI VPKGRFLL
Sbjct: 10  FFFFHLFTQRSSAGSYNVLSFGAKPDGKTDSTQPFLKAWKSACTSPTASTIIVPKGRFLL 69

Query: 72  TPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFGGNIDAKGAGYW 131
           T  TFRGPC + ITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSV GGNIDAKGAGYW
Sbjct: 70  TSTTFRGPCNSHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVVGGNIDAKGAGYW 129

Query: 132 ACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVKAMAPDQS 191
           ACKK G NCP GARSMTFNWANNI++SGLTS+NSQQTHLVIN CNNV VKNVK MAPDQS
Sbjct: 130 ACKKSGNNCPAGARSMTFNWANNIVISGLTSINSQQTHLVINSCNNVKVKNVKVMAPDQS 189

Query: 192 PNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEFN 251
           PNTDGIHVQSST VMI+GSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKE  
Sbjct: 190 PNTDGIHVQSSTNVMITGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKELE 249

Query: 252 EDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKNPIVIDQNYCP- 311
           E+GVENVTLTNAVFIGSDNGVRIKTWP PSKGFV+NVLFQNIIMNNV+NPI+IDQNYCP 
Sbjct: 250 ENGVENVTLTNAVFIGSDNGVRIKTWPRPSKGFVKNVLFQNIIMNNVQNPIIIDQNYCPN 309

Query: 312 NKGCP 316
           N+GCP
Sbjct: 310 NQGCP 314

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BLAST of Spg034899 vs. NCBI nr
Match: XP_004142576.2 (polygalacturonase [Cucumis sativus] >XP_031745382.1 polygalacturonase-like [Cucumis sativus] >KGN66774.1 hypothetical protein Csa_007167 [Cucumis sativus])

HSP 1 Score: 528.9 bits (1361), Expect = 3.1e-146
Identity = 260/312 (83.33%), Postives = 281/312 (90.06%), Query Frame = 0


Query: 1   MANTSMTTSILFF-FFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTP 60
           MAN   T S++F  FF+LFIQ STAANYNV+SFGAKPDGKTDST+ FLKAWTSACSSST 
Sbjct: 1   MANP--TKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTR 60

Query: 61  STINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVF 120
           STINVPKGRFLLTPITFRGPCKNKITF LNGTLVAPLDYRALG+SGYWILFIKVDGVS  
Sbjct: 61  STINVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFV 120

Query: 121 GGNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVW 180
           GG ID KG  YWACK   +NCP GARS+TFNWANNI++SGLTS+NSQQTH+VIN CNNV 
Sbjct: 121 GGTIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVV 180

Query: 181 VKNVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGH 240
           VKNVK MAPDQSPNTDGIHVQSST V I+GST++TGDDCISIGDGTKNLFMS+IKCGPGH
Sbjct: 181 VKNVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMSDIKCGPGH 240

Query: 241 GVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVK 300
           GVSIGSLGKE NE+GVENVTL NAVF  SDNGVRIKTWPTPS GFVR+V+FQNI+M NVK
Sbjct: 241 GVSIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVK 300

Query: 301 NPIVIDQNYCPN 312
           NPI+IDQNYCPN
Sbjct: 301 NPILIDQNYCPN 310

[Close]

BLAST of Spg034899 vs. NCBI nr
Match: XP_031736043.1 (polygalacturonase-like [Cucumis sativus])

HSP 1 Score: 528.9 bits (1361), Expect = 3.1e-146
Identity = 260/312 (83.33%), Postives = 281/312 (90.06%), Query Frame = 0


Query: 1   MANTSMTTSILFF-FFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTP 60
           MAN   T S++F  FF+LFIQ STAANYNV+SFGAKPDGKTDST+ FLKAWTSACSSST 
Sbjct: 1   MANP--TKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTR 60

Query: 61  STINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVF 120
           STINVPKGRFLLTPITFRGPCKNKITF LNGTLVAPLDYRALG+SGYWILFIKVDGVS  
Sbjct: 61  STINVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFV 120

Query: 121 GGNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVW 180
           GG ID KG  YWACK   +NCP GARS+TFNWANNI++SGLTS+NSQQTH+VIN CNNV 
Sbjct: 121 GGTIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVV 180

Query: 181 VKNVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGH 240
           VKNVK MAPDQSPNTDGIHVQSST V I+GST++TGDDCISIGDGTKNLFMS+IKCGPGH
Sbjct: 181 VKNVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMSDIKCGPGH 240

Query: 241 GVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVK 300
           GVSIGSLGKE NE+GVENVTL NAVF  SDNGVRIKTWPTPS GFVR+V+FQNI+M NVK
Sbjct: 241 GVSIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVK 300

Query: 301 NPIVIDQNYCPN 312
           NPI+IDQNYCPN
Sbjct: 301 NPILIDQNYCPN 310

[Close]

BLAST of Spg034899 vs. NCBI nr
Match: XP_022989666.1 (polygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 525.8 bits (1353), Expect = 2.6e-145
Identity = 251/316 (79.43%), Postives = 282/316 (89.24%), Query Frame = 0


Query: 1   MANTSMTTSILFFFFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPS 60
           MANT  T  ++   FY FIQ S+AA YNV+++GAKPDGKTDST+PFLKAWTSAC+SSTPS
Sbjct: 1   MANTIKTLLLIISSFYFFIQLSSAATYNVITYGAKPDGKTDSTRPFLKAWTSACTSSTPS 60

Query: 61  TINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFG 120
           TINVPKGRFLL PITFRGPCKNKITFRLNGTLVAPLD+RALG+SGYWILFIKV+ VS FG
Sbjct: 61  TINVPKGRFLLKPITFRGPCKNKITFRLNGTLVAPLDFRALGSSGYWILFIKVNDVSFFG 120

Query: 121 GNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWV 180
           GNIDAKGA YWACK  G++CP GARS+TFNWANNI +SGLTS+NS+Q+H+ IN CNNV V
Sbjct: 121 GNIDAKGASYWACKDSGKDCPPGARSLTFNWANNIYLSGLTSINSRQSHITINSCNNVLV 180

Query: 181 KNVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHG 240
           KNVK MAPDQSPNTDGIHVQSST V I+G+T+QTGDDCISIGDGTKNLFM++IKCGPGHG
Sbjct: 181 KNVKIMAPDQSPNTDGIHVQSSTDVTITGTTIQTGDDCISIGDGTKNLFMAHIKCGPGHG 240

Query: 241 VSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKN 300
           VSIGSLGKEF+E GV+NVTL NAVF  SDNGVRIKTW  P  GFV++V+F+NIIMNNVKN
Sbjct: 241 VSIGSLGKEFDEQGVQNVTLMNAVFTKSDNGVRIKTWARPGNGFVKHVVFENIIMNNVKN 300

Query: 301 PIVIDQNYCP-NKGCP 316
           PI+IDQNYCP N GCP
Sbjct: 301 PIIIDQNYCPDNVGCP 316

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BLAST of Spg034899 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 2.0e-95
Identity = 178/322 (55.28%), Postives = 221/322 (68.63%), Query Frame = 0


Query: 1   MAN-TSMTTSILFFFFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTP 60
           MAN  S+ +  L F F +    ++   YNV S GAK DGKTDSTK FL AW  AC+S  P
Sbjct: 1   MANRRSLFSLSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNP 60

Query: 61  STINVPKGRFLLTPITFRGPCKNK-ITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSV 120
             I VP G F L  + F GPCKN  ITFR+ GTLVAP DYR +GN+  WI F  V+GV++
Sbjct: 61  GVIYVPAGTFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTI 120

Query: 121 FGGNIDAKGAGYWACKK-FGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNN 180
            GG +D +G   WACK   G +CP GA ++ F+ +NNI+VSGL S+NSQ  H+VIN   N
Sbjct: 121 SGGILDGQGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQN 180

Query: 181 VWVKNVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGP 240
           V ++ V+      SPNTDGIHVQ S+GV I  S + TGDDC+SIG GT NL++  + CGP
Sbjct: 181 VQMQGVRVSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGP 240

Query: 241 GHGVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNN 300
           GHG+SIGSLGKE  E GV+NVT+    F G+ NG+RIK+W  PS GF RN+LFQ+  M N
Sbjct: 241 GHGISIGSLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVN 300

Query: 301 VKNPIVIDQNYCP-NKGCPPKV 319
           V+NPIVIDQ+YCP NKGCP +V
Sbjct: 301 VENPIVIDQHYCPDNKGCPGQV 322

[Close]

BLAST of Spg034899 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 6.7e-91
Identity = 163/291 (56.01%), Postives = 213/291 (73.20%), Query Frame = 0


Query: 28  NVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPSTINVPKGRFLLTPITFRG-PCKN-KIT 87
           NVLS+GAKPDG  DSTK FL AW  AC+S+ P+TI VPKGRFL+  + F G  CK   I+
Sbjct: 34  NVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQAPIS 93

Query: 88  FRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFGGNIDAKGAGYWACK-KFGRNCPVGA 147
            R+ G++VAP D+R + +S +WI F  V  VS++GG +DA+G   W CK   G NCP GA
Sbjct: 94  IRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCPTGA 153

Query: 148 RSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVKAMAPDQSPNTDGIHVQSSTG 207
           +S+ F+ +NNI +SGLTS+NSQ+ H+VI+  NNV +  VK  A + SPNTDGIHV+SS  
Sbjct: 154 KSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVESSHS 213

Query: 208 VMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEFNEDGVENVTLTNAV 267
           V I+ S + TGDDCISIG G+ N+F+  I+CGPGHG+SIGSLG+   E GV+NVT++N  
Sbjct: 214 VHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAEEEQGVDNVTVSNVD 273

Query: 268 FIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKNPIVIDQNYCPNKGCP 316
           F+G++NGVRIKTW   S  F RN++FQ+I M  VKNPI+IDQ+YC +K CP
Sbjct: 274 FMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYCLHKPCP 324

[Close]

BLAST of Spg034899 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-70
Identity = 143/313 (45.69%), Postives = 191/313 (61.02%), Query Frame = 0


Query: 11  LFFFFYLFIQTSTA---ANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPSTINVPKG 70
           +F    LFI  S     A   V  FGAK DGKTD +KPFL AW  AC+S TPST+ +PKG
Sbjct: 11  MFVLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKG 70

Query: 71  RFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFGGNI-DAK 130
            +LL+ +   GPCK  I   + GT+ AP D  A  +   W+ F  V+   +FGG I D +
Sbjct: 71  TYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPN-WVRFYSVENFKMFGGGIFDGQ 130

Query: 131 GAGYW---AC--KKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVK 190
           G+  +    C  ++F    PV  R   F++  N L+  +TS +S+  H+ +  C N+ ++
Sbjct: 131 GSIAYEKNTCENREFRSKLPVNIR---FDFVTNALIQDITSKDSKLFHINVFACKNITLE 190

Query: 191 NVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGV 250
            +K  APD+SPNTDGIH+  S GV I  S ++TGDDCISIGDGTKN+ +  I CGPGHG+
Sbjct: 191 RLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGI 250

Query: 251 SIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKNP 310
           SIGSLGK  NE+ VE + ++N     + NG RIKTWP    G V  + F++I MNNV +P
Sbjct: 251 SIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSP 310

Query: 311 IVIDQNYCPNKGC 315
           I+IDQ YCP   C
Sbjct: 311 ILIDQQYCPWNKC 319

[Close]

BLAST of Spg034899 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-70
Identity = 142/313 (45.37%), Postives = 195/313 (62.30%), Query Frame = 0


Query: 11  LFFFFYLFIQTSTAAN--YNVLS-FGAKPDGKTDSTKPFLKAWTSACSSSTPSTINVPKG 70
           +F    LFI  S   +  ++V++ FGAK DGKTD +KPFL AW  AC+S TPST+ +PKG
Sbjct: 11  MFVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKG 70

Query: 71  RFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFGGNI-DAK 130
            +LL+ +   GPCK  I   + GT+ AP D  A  +   W+ F  V+   +FGG I D +
Sbjct: 71  TYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPN-WVRFYSVENFKMFGGGIFDGQ 130

Query: 131 GAGYW---AC--KKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVK 190
           G+  +    C  ++F    PV  R   F++  N L+  +TS +S+  H+ +  C N+ ++
Sbjct: 131 GSIAYEKNTCENREFRSKLPVNIR---FDFLTNALIQDITSKDSKLFHINVFACKNITLE 190

Query: 191 NVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGV 250
            +K  APD+SPNTDGIH+  S GV I  S ++TGDDCISIGDGTKN+ +  I CGPGHG+
Sbjct: 191 RLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGI 250

Query: 251 SIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKNP 310
           SIGSLGK  NE+ VE + ++N     + NG RIKTWP    G V  + F++I MNNV +P
Sbjct: 251 SIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSP 310

Query: 311 IVIDQNYCPNKGC 315
           I+IDQ YCP   C
Sbjct: 311 ILIDQQYCPWNKC 319

[Close]

BLAST of Spg034899 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.8e-64
Identity = 123/298 (41.28%), Postives = 189/298 (63.42%), Query Frame = 0


Query: 22  STAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPSTINVPKGRFLLTPITFRGPCK 81
           S+ + +NV  +GAK  G  D ++  +KAW +AC+S  PST+ +PKG + +  +  +GPCK
Sbjct: 5   SSGSVFNVNDYGAK--GAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCK 64

Query: 82  -NKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFG-GNIDAKGAGYWA---CKKF 141
            +KI F+++G + AP D     + G W+ F ++DG++V G G +D +G   WA   C K 
Sbjct: 65  GSKIGFQIDGVVKAPADPSKFKSDG-WVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDK- 124

Query: 142 GRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVKAMAPDQSPNTDG 201
             NC   A ++ F++  + +V  +TS+NS+  H+ +  C ++  ++V   AP  S NTDG
Sbjct: 125 NPNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDG 184

Query: 202 IHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEFNEDGVE 261
           IHV  S GV I+ + + TGDDCISIG G++N+ ++ + CGPGHG+SIGSLG+  NE  V 
Sbjct: 185 IHVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVR 244

Query: 262 NVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKNPIVIDQNYCPNKGC 315
            +T+    F G+ NGVR+KTWP    G   ++ FQ++ MNNV+NP+++DQ YCP   C
Sbjct: 245 GITVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQC 298

[Close]

BLAST of Spg034899 vs. ExPASy TrEMBL
Match: A0A6J1DRI6 (polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111023701 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 9.8e-154
Identity = 267/305 (87.54%), Postives = 282/305 (92.46%), Query Frame = 0


Query: 12  FFFFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPSTINVPKGRFLL 71
           FFFF+LF Q S+A +YNVLSFGAKPDGKTDST+PFLKAW SAC+S T STI VPKGRFLL
Sbjct: 10  FFFFHLFTQRSSAGSYNVLSFGAKPDGKTDSTQPFLKAWKSACTSPTASTIIVPKGRFLL 69

Query: 72  TPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFGGNIDAKGAGYW 131
           T  TFRGPC + ITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSV GGNIDAKGAGYW
Sbjct: 70  TSTTFRGPCNSHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVVGGNIDAKGAGYW 129

Query: 132 ACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVKAMAPDQS 191
           ACKK G NCP GARSMTFNWANNI++SGLTS+NSQQTHLVIN CNNV VKNVK MAPDQS
Sbjct: 130 ACKKSGNNCPAGARSMTFNWANNIVISGLTSINSQQTHLVINSCNNVKVKNVKVMAPDQS 189

Query: 192 PNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEFN 251
           PNTDGIHVQSST VMI+GSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKE  
Sbjct: 190 PNTDGIHVQSSTNVMITGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKELE 249

Query: 252 EDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKNPIVIDQNYCP- 311
           E+GVENVTLTNAVFIGSDNGVRIKTWP PSKGFV+NVLFQNIIMNNV+NPI+IDQNYCP 
Sbjct: 250 ENGVENVTLTNAVFIGSDNGVRIKTWPRPSKGFVKNVLFQNIIMNNVQNPIIIDQNYCPN 309

Query: 312 NKGCP 316
           N+GCP
Sbjct: 310 NQGCP 314

[Close]

BLAST of Spg034899 vs. ExPASy TrEMBL
Match: A0A0A0M0F3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G689640 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.5e-146
Identity = 260/312 (83.33%), Postives = 281/312 (90.06%), Query Frame = 0


Query: 1   MANTSMTTSILFF-FFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTP 60
           MAN   T S++F  FF+LFIQ STAANYNV+SFGAKPDGKTDST+ FLKAWTSACSSST 
Sbjct: 1   MANP--TKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTR 60

Query: 61  STINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVF 120
           STINVPKGRFLLTPITFRGPCKNKITF LNGTLVAPLDYRALG+SGYWILFIKVDGVS  
Sbjct: 61  STINVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFV 120

Query: 121 GGNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVW 180
           GG ID KG  YWACK   +NCP GARS+TFNWANNI++SGLTS+NSQQTH+VIN CNNV 
Sbjct: 121 GGTIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVV 180

Query: 181 VKNVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGH 240
           VKNVK MAPDQSPNTDGIHVQSST V I+GST++TGDDCISIGDGTKNLFMS+IKCGPGH
Sbjct: 181 VKNVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMSDIKCGPGH 240

Query: 241 GVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVK 300
           GVSIGSLGKE NE+GVENVTL NAVF  SDNGVRIKTWPTPS GFVR+V+FQNI+M NVK
Sbjct: 241 GVSIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVK 300

Query: 301 NPIVIDQNYCPN 312
           NPI+IDQNYCPN
Sbjct: 301 NPILIDQNYCPN 310

[Close]

BLAST of Spg034899 vs. ExPASy TrEMBL
Match: A0A6J1JKS6 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486681 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 251/316 (79.43%), Postives = 282/316 (89.24%), Query Frame = 0


Query: 1   MANTSMTTSILFFFFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPS 60
           MANT  T  ++   FY FIQ S+AA YNV+++GAKPDGKTDST+PFLKAWTSAC+SSTPS
Sbjct: 1   MANTIKTLLLIISSFYFFIQLSSAATYNVITYGAKPDGKTDSTRPFLKAWTSACTSSTPS 60

Query: 61  TINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFG 120
           TINVPKGRFLL PITFRGPCKNKITFRLNGTLVAPLD+RALG+SGYWILFIKV+ VS FG
Sbjct: 61  TINVPKGRFLLKPITFRGPCKNKITFRLNGTLVAPLDFRALGSSGYWILFIKVNDVSFFG 120

Query: 121 GNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWV 180
           GNIDAKGA YWACK  G++CP GARS+TFNWANNI +SGLTS+NS+Q+H+ IN CNNV V
Sbjct: 121 GNIDAKGASYWACKDSGKDCPPGARSLTFNWANNIYLSGLTSINSRQSHITINSCNNVLV 180

Query: 181 KNVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHG 240
           KNVK MAPDQSPNTDGIHVQSST V I+G+T+QTGDDCISIGDGTKNLFM++IKCGPGHG
Sbjct: 181 KNVKIMAPDQSPNTDGIHVQSSTDVTITGTTIQTGDDCISIGDGTKNLFMAHIKCGPGHG 240

Query: 241 VSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKN 300
           VSIGSLGKEF+E GV+NVTL NAVF  SDNGVRIKTW  P  GFV++V+F+NIIMNNVKN
Sbjct: 241 VSIGSLGKEFDEQGVQNVTLMNAVFTKSDNGVRIKTWARPGNGFVKHVVFENIIMNNVKN 300

Query: 301 PIVIDQNYCP-NKGCP 316
           PI+IDQNYCP N GCP
Sbjct: 301 PIIIDQNYCPDNVGCP 316

[Close]

BLAST of Spg034899 vs. ExPASy TrEMBL
Match: A0A6J1JGH0 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486682 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 4.1e-144
Identity = 247/316 (78.16%), Postives = 282/316 (89.24%), Query Frame = 0


Query: 1   MANTSMTTSILFFFFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPS 60
           MANT     ++   FY FIQ S+AA YNV+++GAKPDGKTDST+PFLKAWTSAC+SSTPS
Sbjct: 1   MANTIKALLLIISSFYFFIQLSSAATYNVITYGAKPDGKTDSTRPFLKAWTSACTSSTPS 60

Query: 61  TINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFG 120
           TI+VPKGRFLL PITFRGPCKNKITFRLNGTLVAPLD+RALG+SGYWILFIKV+ VSVFG
Sbjct: 61  TISVPKGRFLLKPITFRGPCKNKITFRLNGTLVAPLDFRALGSSGYWILFIKVNDVSVFG 120

Query: 121 GNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWV 180
           GNIDAKGA YWAC+  G++CP GARS+TFNWANNI +SGLTS+NS+Q+H+ IN CNNV V
Sbjct: 121 GNIDAKGASYWACRDSGKDCPPGARSLTFNWANNIYLSGLTSINSRQSHITINSCNNVLV 180

Query: 181 KNVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHG 240
           KNVK MAPDQSPNTDGIHVQSST V I+G+T+QTGDDCISIGDGTKNLFM++IKCGPGHG
Sbjct: 181 KNVKIMAPDQSPNTDGIHVQSSTDVTITGTTIQTGDDCISIGDGTKNLFMTHIKCGPGHG 240

Query: 241 VSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKN 300
           VSIGSLGKEF+E GV+NVTL NAVF  SDNGVRIKTW  P  G+V++V+F+NI+MNNVKN
Sbjct: 241 VSIGSLGKEFDEQGVQNVTLMNAVFTKSDNGVRIKTWARPGNGYVKHVVFENIVMNNVKN 300

Query: 301 PIVIDQNYCP-NKGCP 316
           PI+IDQNYCP N GCP
Sbjct: 301 PIIIDQNYCPDNIGCP 316

[Close]

BLAST of Spg034899 vs. ExPASy TrEMBL
Match: A0A6J1JN08 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486680 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.0e-144
Identity = 247/316 (78.16%), Postives = 279/316 (88.29%), Query Frame = 0


Query: 1   MANTSMTTSILFFFFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPS 60
           MANT     ++   FY FIQ S+AA YNV+++GAKPDGKTDST+PFLKAWTSAC+SSTPS
Sbjct: 1   MANTIKALLLIISSFYFFIQLSSAATYNVITYGAKPDGKTDSTRPFLKAWTSACTSSTPS 60

Query: 61  TINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFG 120
           TINVPKGRFLL PITFRGPCKNKITFRLNGTLVAPLD+RALG+SGYWILFIKV  VS FG
Sbjct: 61  TINVPKGRFLLKPITFRGPCKNKITFRLNGTLVAPLDFRALGSSGYWILFIKVTDVSFFG 120

Query: 121 GNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWV 180
           GNIDAKGA YWAC+  G++CP GARS+TFNWANNI +SGLTS+NS+Q+H+ IN CNNV V
Sbjct: 121 GNIDAKGASYWACRDSGKDCPPGARSLTFNWANNIYLSGLTSINSRQSHITINSCNNVLV 180

Query: 181 KNVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHG 240
           KNVK MAPDQSPNTDGIHVQSST V I+G+T+QTGDDCISIGDGTKNLFM++IKCGPGHG
Sbjct: 181 KNVKIMAPDQSPNTDGIHVQSSTDVTITGTTIQTGDDCISIGDGTKNLFMAHIKCGPGHG 240

Query: 241 VSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKN 300
           VSIGSLGKEF+E GV+NVTL N VF  SDNGVRIKTW  P  GFV++V+F+NI+MNNVKN
Sbjct: 241 VSIGSLGKEFDEQGVQNVTLMNTVFTKSDNGVRIKTWARPGNGFVKHVVFENIVMNNVKN 300

Query: 301 PIVIDQNYCP-NKGCP 316
           PI+IDQNYCP N GCP
Sbjct: 301 PIIIDQNYCPDNVGCP 316

[Close]

BLAST of Spg034899 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 457.6 bits (1176), Expect = 8.3e-129
Identity = 210/315 (66.67%), Postives = 262/315 (83.17%), Query Frame = 0


Query: 3   NTSMTTSILFFFFYLFIQTSTAA-NYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPST 62
           N  +   ++FF  +L +++STAA NYNV+SFGAKPDG+TDSTK FL AW +AC S+   T
Sbjct: 4   NKLVAVLLMFFSSFLLMKSSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAAAVT 63

Query: 63  INVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFGG 122
           + VP+G FLL P+ FRGPC+++ITF++ GT+VAP DYR LGNSGYWILF+KV+ +S+ GG
Sbjct: 64  VTVPRGSFLLKPVEFRGPCRSRITFQIYGTIVAPSDYRGLGNSGYWILFVKVNRISIIGG 123

Query: 123 NIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVK 182
            +DA+GA +WAC+K G++CPVGARSMTFNWAN+++VSGLTS+NSQ THLVIN CNNV V+
Sbjct: 124 TLDARGASFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLVINSCNNVIVR 183

Query: 183 NVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGV 242
            VK +APDQSPNTDG+HVQ S GV ++  T  TGDDCISIG GT+NL+MS + CGPGHG+
Sbjct: 184 KVKLVAPDQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKLNCGPGHGI 243

Query: 243 SIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKNP 302
           SIGSLG++ NE GVEN+TL N+VF GSDNGVRIKTW   S GFVRNVLFQN+IM NV+NP
Sbjct: 244 SIGSLGRDANEAGVENITLINSVFSGSDNGVRIKTWARQSTGFVRNVLFQNLIMKNVQNP 303

Query: 303 IVIDQNYCP-NKGCP 316
           I++DQNYCP N+GCP
Sbjct: 304 IIVDQNYCPSNQGCP 318

[Close]

BLAST of Spg034899 vs. TAIR 10
Match: AT1G05650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 404.4 bits (1038), Expect = 8.3e-113
Identity = 191/314 (60.83%), Postives = 244/314 (77.71%), Query Frame = 0


Query: 5   SMTTSILFFFFYLFIQTSTAAN--YNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPSTI 64
           S  T  L F    FI  S++A+  +NV+SFGAKPDG TDST  FLKAW  AC S+  +T+
Sbjct: 4   SAITFPLIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASATV 63

Query: 65  NVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFGGN 124
            VP G FLL  ITF GPCK+KITF++ GT+VAP DYR  GNSG WILF KV+  S+ GG 
Sbjct: 64  VVPTGTFLLKVITFGGPCKSKITFQVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLVGGT 123

Query: 125 IDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKN 184
            DA+G+G+W+C+K G+NCP G RS++FN A ++++SG+ S+NSQ +H+ +NGC NV V+N
Sbjct: 124 FDARGSGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVSHMTLNGCTNVAVRN 183

Query: 185 VKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVS 244
           ++ +AP  SPNTDG  VQ STGV ++GST+QTGDDC++IG GT+N  +S + CGPGHGVS
Sbjct: 184 IRLVAPGDSPNTDGFTVQFSTGVTLTGSTVQTGDDCVAIGQGTRNFLISKLACGPGHGVS 243

Query: 245 IGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKNPI 304
           IGSL K+ NEDGVENVT++++VF GS NGVRIK+W  PS GFVRNV FQN+IM NV+NPI
Sbjct: 244 IGSLAKQLNEDGVENVTVSSSVFTGSQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQNPI 303

Query: 305 VIDQNYCP-NKGCP 316
           +IDQNYCP N+GCP
Sbjct: 304 IIDQNYCPSNQGCP 317

[Close]

BLAST of Spg034899 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 401.4 bits (1030), Expect = 7.0e-112
Identity = 191/317 (60.25%), Postives = 245/317 (77.29%), Query Frame = 0


Query: 6   MTTSI----LFFFFYLFIQTSTAAN--YNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTP 65
           MT S+    L F    FI  S +A+  +NV+SFGAKPDG TDST  FLKAW  AC S++ 
Sbjct: 1   MTKSVIRFSLLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASS 60

Query: 66  STINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVF 125
           +T+ VPKG FLL  ITF GPCK+KITF++ GT++AP DYR  GNSG+WILF KV+  S+ 
Sbjct: 61  ATVVVPKGTFLLKVITFGGPCKSKITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLV 120

Query: 126 GGNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVW 185
           GG  DA+  G+W+C+K G+NCP G RS++FN A ++++SG+ S+NSQ TH+ +NGC NV 
Sbjct: 121 GGTFDARANGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVV 180

Query: 186 VKNVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGH 245
           V+NVK +AP  SPNTDG HVQ STGV  +GST+QTGDDC++IG GT+NL ++ + CGPGH
Sbjct: 181 VRNVKLVAPGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPGH 240

Query: 246 GVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVK 305
           GVSIGSL KE  EDGVENVT++++VF GS NGVRIK+W  PS GFVR V FQ+++M NV+
Sbjct: 241 GVSIGSLAKELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNVE 300

Query: 306 NPIVIDQNYCP-NKGCP 316
           NPI+IDQNYCP ++GCP
Sbjct: 301 NPIIIDQNYCPTHEGCP 317

[Close]

BLAST of Spg034899 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 395.6 bits (1015), Expect = 3.9e-110
Identity = 185/312 (59.29%), Postives = 238/312 (76.28%), Query Frame = 0


Query: 9   SILFFFFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTPSTINVPKGR 68
           S+L  F + FI + +A +YNVLSFGAKPDGKTD+TK F+  W +AC+SS P TI VPKGR
Sbjct: 3   SLLVLFVFFFISSCSAQSYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGR 62

Query: 69  FLLTPITFRG-PCKNK-ITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVFGGNIDAK 128
           FLL  +TF G  CK K +TFR++GTLVAP DYR +GN  YWI F  +DG++V+GG +DA+
Sbjct: 63  FLLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDAR 122

Query: 129 GAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVKAM 188
           GA  W CKK G+NCP GA ++ F  ++N++VSGLTS+NSQ  H+VINGCNNV ++ VK +
Sbjct: 123 GASLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKVL 182

Query: 189 APDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSL 248
           A   SPNTDGIHVQSS+ V I  + + TGDDC+SIG GT  L++ N+ CGPGHG+SIGSL
Sbjct: 183 AAGNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSL 242

Query: 249 GKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVKNPIVIDQ 308
           GK+  E GV+NVT+    F G+DNGVRIK+W  PS GF +N+ FQ+ +MNNV+NPI+IDQ
Sbjct: 243 GKDSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIIDQ 302

Query: 309 NYCPNKGCPPKV 319
           NYCP+  CP +V
Sbjct: 303 NYCPDHDCPRQV 314

[Close]

BLAST of Spg034899 vs. TAIR 10
Match: AT2G43880.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 387.9 bits (995), Expect = 8.0e-108
Identity = 178/317 (56.15%), Postives = 243/317 (76.66%), Query Frame = 0


Query: 3   NTSMTTSILFF---FFYLFIQTSTAANYNVLSFGAKPDGKTDSTKPFLKAWTSACSSSTP 62
           N S   +I+FF    F+L   +    ++NV  +GA+ DG+ D+TK FL AW+ AC S   
Sbjct: 4   NISFPCAIIFFSINIFFLIKSSHAMPSFNVQRYGARGDGRADATKSFLTAWSLACGSRAR 63

Query: 63  STINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVF 122
           + + VP+G +L+  + F GPCKN ITF+ +GTLVAP +Y  +GNSGYWILF KV+ +SV+
Sbjct: 64  AMVYVPRGTYLVKNLVFWGPCKNIITFKNDGTLVAPANYWDIGNSGYWILFAKVNRISVY 123

Query: 123 GGNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVW 182
           GG IDA+GAGYW+C+K G +CP GARS++F+W NN+L+SGL+S NSQ  H+ ++  +NV 
Sbjct: 124 GGTIDARGAGYWSCRKKGSHCPQGARSISFSWCNNVLLSGLSSFNSQNMHVTVHHSSNVR 183

Query: 183 VKNVKAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGH 242
           ++NV+  AP  SPNTDGIHVQSS+GV ISG T+ TGDDCI++  G++N+++  + CGPGH
Sbjct: 184 IENVRIRAPSGSPNTDGIHVQSSSGVTISGGTIATGDDCIALSQGSRNIWIERVNCGPGH 243

Query: 243 GVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGFVRNVLFQNIIMNNVK 302
           G+SIGSLG   NE+GV+NVT+T++VF  + NGVRIKTW  PS+GFV NV+F+N+IMNNV+
Sbjct: 244 GISIGSLGDYANEEGVQNVTVTSSVFTKTQNGVRIKTWARPSRGFVNNVVFRNLIMNNVE 303

Query: 303 NPIVIDQNYCPN-KGCP 316
           NP++IDQNYCPN KGCP
Sbjct: 304 NPVIIDQNYCPNGKGCP 320

The following BLAST results are available for this feature:

BLAST of Spg034899 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888873.1	9.7e-156	86.44	polygalacturonase-like [Benincasa hispida] >XP_038888876.1 polygalacturonase-lik...	[more]
XP_022156860.1	2.0e-153	87.54	polygalacturonase-like [Momordica charantia]	[more]
XP_004142576.2	3.1e-146	83.33	polygalacturonase [Cucumis sativus] >XP_031745382.1 polygalacturonase-like [Cucu...	[more]
XP_031736043.1	3.1e-146	83.33	polygalacturonase-like [Cucumis sativus]	[more]
XP_022989666.1	2.6e-145	79.43	polygalacturonase-like [Cucurbita maxima]	[more]

BLAST of Spg034899 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P48979	2.0e-95	55.28	Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1	[more]
O22818	6.7e-91	56.01	Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386...	[more]
Q39766	1.3e-70	45.69	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]
Q39786	1.3e-70	45.37	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]
Q6H9K0	1.8e-64	41.28	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]

BLAST of Spg034899 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DRI6	9.8e-154	87.54	polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111023701 PE=3 SV=1	[more]
A0A0A0M0F3	1.5e-146	83.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G689640 PE=3 SV=1	[more]
A0A6J1JKS6	1.3e-145	79.43	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486681 PE=3 SV=1	[more]
A0A6J1JGH0	4.1e-144	78.16	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486682 PE=3 SV=1	[more]
A0A6J1JN08	7.0e-144	78.16	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486680 PE=3 SV=1	[more]

BLAST of Spg034899 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G43890.1	8.3e-129	66.67	Pectin lyase-like superfamily protein	[more]
AT1G05650.1	8.3e-113	60.83	Pectin lyase-like superfamily protein	[more]
AT1G05660.1	7.0e-112	60.25	Pectin lyase-like superfamily protein	[more]
AT2G43870.1	3.9e-110	59.29	Pectin lyase-like superfamily protein	[more]
AT2G43880.1	8.0e-108	56.15	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 202..223 e-value: 200.0 score: 10.8 coord: 175..201 e-value: 230.0 score: 10.6 coord: 255..276 e-value: 16.0 score: 14.4 coord: 285..306 e-value: 3500.0 score: 3.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 17..322 e-value: 8.7E-97 score: 326.7
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 54..315 e-value: 3.5E-75 score: 253.1
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 232..245
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 9..317
None	No IPR available	PANTHER	PTHR31375:SF31	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 9..317
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 12..318

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg034899.1	Spg034899.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity