Spg034872 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg034872
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	tRNA (Guanine(10)-N2)-methyltransferase homolog
Location	scaffold3: 24571983 .. 24573535 (+)
RNA-Seq Expression	Spg034872
Synteny	Spg034872

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTGTCGTGTGTCCGTGCTTCTTAGGTGTTCGGAGGTTGTGACGGCCTTATGGGAAGTCTCGATGCCCTATGTAAGTAAGATTTTCTGATTTAGGACTCTTTTTTGTGGCAGTTGATACATCGGCGAGGGATTACAAGCTAGGTTTCCAAATATGGTACTTGTGTGCACCATCAAATTCCCATTTACTTTTTTGCCCTGTTTTTTTTTAACCATAACTTTTGCAATTGTTCCTCTGGGGACATCCGATAAAATTGGAATGACACAGAAAATGTTCGCAATTTGGTAGAACTTGTACTTTTATGGGTTGTTTGAAAGACGTCACCAGGCACACGAGGAGAAAGATATGGTGAAAACAACATACCCTTCCGTGGGACAACATTTTAAATACCTGTTGATCCGATGTTTATTAAAAGTTGTAACATTATACTGGCTGTCCTGAGAGTCTTCGATTCTGAGGCCTGGTTATTCAGCTACTGTTACTTCTCAATGCTGATTCTGATGCATTTTCATAACTTGTTTCTTAACCCAGATATTTGATGCAATCATTTGTGACCCTCCTTATGGAGTTCGTGCCGGGGGGCGTAAATCCGGTGGTCGGAAATTGTTAAAAGGAACTGTTGCCCCTTACACCGTTCCAGATGACAAGAGAACAGACCATATACCATCAACTGCAGCTTACAGCCTTGTTGAGTGTGTGCATGATTTACTTGACCTTGCTGCCAGGATGCTTGTGATGGGTGGCAGGCTTGTATTCTTCTACCCTGTGTTGAGGGAAGAAGATTGTGTGTTTAACCAATTCCCAGAGCATCCATGTTTTGCTTTAATCAGTTCTTCCGAGCAGATACTGAGCTCTCGATACAGCCGGGTGTTGCTAACCATGGTCAAAAGAAGTTCATACACTGAAGAAATTGCGGAGGCAGCAAGATTAAAGCATGAAGAATTCAGAGAGAACCATTTGAAGTGGTTAGAGGATGGTAATCTCCATTCTGCCGTTTTCAGTCCTGCCACTGATCCCGATCTTGCAGTTTCTGGTAATGGCAATGTGAGTAAAGACCCGAAACCGAAATACAGAGGGAAGTATGTATAAATGGAAGTCAGTGTAGGCGTTGGTTTTGCTTGTTCTCTAAATTATCTAAGCCTGGATTTTGGTTCATGTGCATGCAGTTTTTTCTGGGAGTAGGAGAGTAGGAAGGACCTTTGATTAGCTTTTCGAGAAATTTTCCCATGTCTGAATTGAAATTGAGATACTGATACAAGTTCGGGAGTCTCTCCAAATCTTGAGTTTATATCATGGAGAGAAGTCTTGCATTGAATATCTCAGCATATGCATGGTTTAGTTTCAATGTTGATCACCAGTTCTCTAAACCCTACTGAAATGGTATATGAGAGATTTTTATAAAAATGTAATAATTTTAGACGAGAACTTTAGTTATTACGTGAGAATTTTACAAGTATTTATGTTTTTTTTTCCTTCTGTATGTGAATCTGTATCATTTTTTTTTTTGTAATTTAGTTTTTTAACTTAATTATTACAACAATCATTTTCCC

mRNA sequence

ATGTCGTGTCGTGTGTCCGTGCTTCTTAGGTGTTCGGAGGTTGTGACGGCCTTATGGGAAGTCTCGATGCCCTATATATTTGATGCAATCATTTGTGACCCTCCTTATGGAGTTCGTGCCGGGGGGCGTAAATCCGGTGGTCGGAAATTGTTAAAAGGAACTGTTGCCCCTTACACCGTTCCAGATGACAAGAGAACAGACCATATACCATCAACTGCAGCTTACAGCCTTGTTGAGTGTGTGCATGATTTACTTGACCTTGCTGCCAGGATGCTTGTGATGGGTGGCAGGCTTGTATTCTTCTACCCTGTGTTGAGGGAAGAAGATTGTGTGTTTAACCAATTCCCAGAGCATCCATGTTTTGCTTTAATCAGTTCTTCCGAGCAGATACTGAGCTCTCGATACAGCCGGGTGTTGCTAACCATGGTCAAAAGAAGTTCATACACTGAAGAAATTGCGGAGGCAGCAAGATTAAAGCATGAAGAATTCAGAGAGAACCATTTGAAGTGGTTAGAGGATGGTAATCTCCATTCTGCCGTTTTCAGTCCTGCCACTGATCCCGATCTTGCAGTTTCTGGTAATGGCAATGTGAGTAAAGACCCGAAACCGAAATACAGAGGGAAGTATGTATAA

Coding sequence (CDS)

ATGTCGTGTCGTGTGTCCGTGCTTCTTAGGTGTTCGGAGGTTGTGACGGCCTTATGGGAAGTCTCGATGCCCTATATATTTGATGCAATCATTTGTGACCCTCCTTATGGAGTTCGTGCCGGGGGGCGTAAATCCGGTGGTCGGAAATTGTTAAAAGGAACTGTTGCCCCTTACACCGTTCCAGATGACAAGAGAACAGACCATATACCATCAACTGCAGCTTACAGCCTTGTTGAGTGTGTGCATGATTTACTTGACCTTGCTGCCAGGATGCTTGTGATGGGTGGCAGGCTTGTATTCTTCTACCCTGTGTTGAGGGAAGAAGATTGTGTGTTTAACCAATTCCCAGAGCATCCATGTTTTGCTTTAATCAGTTCTTCCGAGCAGATACTGAGCTCTCGATACAGCCGGGTGTTGCTAACCATGGTCAAAAGAAGTTCATACACTGAAGAAATTGCGGAGGCAGCAAGATTAAAGCATGAAGAATTCAGAGAGAACCATTTGAAGTGGTTAGAGGATGGTAATCTCCATTCTGCCGTTTTCAGTCCTGCCACTGATCCCGATCTTGCAGTTTCTGGTAATGGCAATGTGAGTAAAGACCCGAAACCGAAATACAGAGGGAAGTATGTATAA

Protein sequence

MSCRVSVLLRCSEVVTALWEVSMPYIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRVLLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPATDPDLAVSGNGNVSKDPKPKYRGKYV

Homology

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BLAST of Spg034872 vs. NCBI nr
Match: XP_038888658.1 (tRNA (guanine(10)-N2)-methyltransferase homolog [Benincasa hispida])

HSP 1 Score: 364.4 bits (934), Expect = 6.7e-97
Identity = 176/192 (91.67%), Postives = 182/192 (94.79%), Query Frame = 0


Query: 19  WEVSMPYIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 78
           W   +  +FDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV
Sbjct: 289 WRPGLKEMFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 348

Query: 79  ECVHDLLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRV 138
           ECVHDLLDLAA+MLVMGGRLVFFYPVLREEDCVFNQFPEHPCF LISSSEQILSSRYSRV
Sbjct: 349 ECVHDLLDLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRV 408

Query: 139 LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPATDPDLAVSGNGNVS 198
           LLTMVKRSSYTEE+AEAARLKHEEFRENHLKWLEDGNLHSAVFSP+TD  LAV GN N+S
Sbjct: 409 LLTMVKRSSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSTDSQLAVGGNCNLS 468

Query: 199 KDPKPKYRGKYV 211
           KDPKPKYRGKYV
Sbjct: 469 KDPKPKYRGKYV 480

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BLAST of Spg034872 vs. NCBI nr
Match: XP_031744574.1 (tRNA (guanine(10)-N2)-methyltransferase homolog [Cucumis sativus])

HSP 1 Score: 361.3 bits (926), Expect = 5.6e-96
Identity = 173/192 (90.10%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 19  WEVSMPYIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 78
           W   +  IFDAIICDPPYGVRAGGRKSGGRKLLKG VAPYTVPDDKRTDHIPSTA+YSLV
Sbjct: 289 WRPGLKEIFDAIICDPPYGVRAGGRKSGGRKLLKGIVAPYTVPDDKRTDHIPSTASYSLV 348

Query: 79  ECVHDLLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRV 138
           ECVHDLLDLAA+MLVMGGRLVFFYP+LREEDCVFNQFPEHPCF  ISSSEQILSSRYSRV
Sbjct: 349 ECVHDLLDLAAKMLVMGGRLVFFYPMLREEDCVFNQFPEHPCFTSISSSEQILSSRYSRV 408

Query: 139 LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPATDPDLAVSGNGNVS 198
           LLTMVKRSSYTEE+AEAARLKHEEFRENHLKWLEDGNLHSAVFSP+ +P L V+GNGNVS
Sbjct: 409 LLTMVKRSSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSVNPQLGVAGNGNVS 468

Query: 199 KDPKPKYRGKYV 211
           KDPKPKYRGKYV
Sbjct: 469 KDPKPKYRGKYV 480

[Close]

BLAST of Spg034872 vs. NCBI nr
Match: XP_008447182.1 (PREDICTED: tRNA (guanine(10)-N2)-methyltransferase homolog [Cucumis melo])

HSP 1 Score: 361.3 bits (926), Expect = 5.6e-96
Identity = 174/192 (90.62%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 19  WEVSMPYIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 78
           W   +  IFDAIICDPPYGVRAGGRKSGGRKLLKG VAPYTVPDDKRTDHIPSTA+YSLV
Sbjct: 289 WRPGLKEIFDAIICDPPYGVRAGGRKSGGRKLLKGIVAPYTVPDDKRTDHIPSTASYSLV 348

Query: 79  ECVHDLLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRV 138
           ECVHDLLDLAA+MLVMGGRLVFFYP+LREEDCVFNQFPEHPCF LISSSEQILSSRYSRV
Sbjct: 349 ECVHDLLDLAAKMLVMGGRLVFFYPMLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRV 408

Query: 139 LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPATDPDLAVSGNGNVS 198
           LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSP+ +  L V+GNGNVS
Sbjct: 409 LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSVNRQLGVAGNGNVS 468

Query: 199 KDPKPKYRGKYV 211
           KDPKPKYRGKYV
Sbjct: 469 KDPKPKYRGKYV 480

[Close]

BLAST of Spg034872 vs. NCBI nr
Match: XP_022932935.1 (tRNA (guanine(10)-N2)-methyltransferase homolog [Cucurbita moschata] >XP_023511692.1 tRNA (guanine(10)-N2)-methyltransferase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 359.8 bits (922), Expect = 1.6e-95
Identity = 173/192 (90.10%), Postives = 180/192 (93.75%), Query Frame = 0


Query: 19  WEVSMPYIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 78
           W   +  IFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV
Sbjct: 289 WRPGLKEIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 348

Query: 79  ECVHDLLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRV 138
           ECVHDLL+LAA+MLVMGGRLVFFYPVLREEDCVFNQFPEHPCF LISSSEQILSSRYSRV
Sbjct: 349 ECVHDLLNLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRV 408

Query: 139 LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPATDPDLAVSGNGNVS 198
           LLTMVK SSYTEE+AEAA+LKHEEFRENHLKWLEDGNLHSAVFSP  DP LAV+GN N +
Sbjct: 409 LLTMVKHSSYTEEMAEAAKLKHEEFRENHLKWLEDGNLHSAVFSPTIDPQLAVAGNCNAT 468

Query: 199 KDPKPKYRGKYV 211
           KDPKPKYRGKYV
Sbjct: 469 KDPKPKYRGKYV 480

[Close]

BLAST of Spg034872 vs. NCBI nr
Match: KAG7011460.1 (tRNA (guanine(10)-N2)-methyltransferase-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 359.0 bits (920), Expect = 2.8e-95
Identity = 172/192 (89.58%), Postives = 180/192 (93.75%), Query Frame = 0


Query: 19  WEVSMPYIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 78
           W   +  IFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV
Sbjct: 289 WRPGLKEIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 348

Query: 79  ECVHDLLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRV 138
           ECVHDLL+LAA+MLVMGGRLVFFYPVLREEDCVFNQFPEHPCF L+SSSEQILSSRYSRV
Sbjct: 349 ECVHDLLNLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLLSSSEQILSSRYSRV 408

Query: 139 LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPATDPDLAVSGNGNVS 198
           LLTMVK SSYTEE+AEAA+LKHEEFRENHLKWLEDGNLHSAVFSP  DP LAV+GN N +
Sbjct: 409 LLTMVKHSSYTEEMAEAAKLKHEEFRENHLKWLEDGNLHSAVFSPTIDPQLAVAGNCNAT 468

Query: 199 KDPKPKYRGKYV 211
           KDPKPKYRGKYV
Sbjct: 469 KDPKPKYRGKYV 480

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BLAST of Spg034872 vs. ExPASy Swiss-Prot
Match: Q54QA6 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Dictyostelium discoideum OX=44689 GN=trmt11 PE=3 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 8.1e-21
Identity = 61/145 (42.07%), Postives = 86/145 (59.31%), Query Frame = 0


Query: 19  WEVSMPYIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 78
           W V+   +FD+II DPPYG+RAG RK G ++  K       VP+  R DHIP    YS+ 
Sbjct: 299 WRVNS--MFDSIITDPPYGIRAGARKIGFKENRKFV----PVPEGLRRDHIPQCIDYSVP 358

Query: 79  ECVHDLLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSS-EQILSSRYSR 138
           + + DLL+LAA+ L++GGRLV++ P     D      P HPC  LI++S  QIL++R+ R
Sbjct: 359 DVMADLLELAAKTLIVGGRLVYWLPT--TPDYKETDLPRHPCLRLITASCLQILTNRWGR 418

Query: 139 VLLTMVKRSSYTEEIAEAARLKHEE 163
            L+TM K   Y + I   + L  E+
Sbjct: 419 RLVTMEKIIEYNDSIHNKSLLVQED 435

[Close]

BLAST of Spg034872 vs. ExPASy Swiss-Prot
Match: Q6NS23 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Xenopus laevis OX=8355 GN=trmt11 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 4.9e-18
Identity = 61/156 (39.10%), Postives = 81/156 (51.92%), Query Frame = 0


Query: 26  IFDAIICDPPYGVRAGGRKSGGRK-LLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDL 85
           +FDAII DPPYG+R   RK+G +K ++K  + P          H+P    Y L +   DL
Sbjct: 305 LFDAIITDPPYGIRESTRKTGTQKEIIKTELFP--------ESHVPVQLNYHLSDIFSDL 364

Query: 86  LDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRVLLTMVK 145
              A+  LV GGRLV++ PV R E       P HPC  LIS+ EQ+LSS  SR L+TM K
Sbjct: 365 FAFASEFLVTGGRLVYWLPVYRPE-YTEEVLPRHPCLKLISNCEQMLSSHTSRRLITMEK 424

Query: 146 RSSYTEE-----IAEAARLK---HEEFRENHLKWLE 173
              + E+     + E   L    H  FRE +   L+
Sbjct: 425 VKEFEEKYQYAHLGEGQNLPYKGHNSFREKYFSGLK 451

[Close]

BLAST of Spg034872 vs. ExPASy Swiss-Prot
Match: Q6YJI5 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Gallus gallus OX=9031 GN=TRMT11 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.1e-17
Identity = 60/152 (39.47%), Postives = 83/152 (54.61%), Query Frame = 0


Query: 26  IFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRT--DHIPSTAAYSLVECVHD 85
           +FDAII DPPYG+R   R++G +K         +V   +R+  +HI  +++Y L +   D
Sbjct: 302 LFDAIITDPPYGIREATRRTGSQK--------ESVKSSERSTENHIIISSSYHLSDIFFD 361

Query: 86  LLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRVLLTMV 145
           LL  AA  LVMGGRLV++ P+ R E       P HPC  LIS+ EQ+LSS  SR L+TM 
Sbjct: 362 LLKFAAEYLVMGGRLVYWLPIYRPE-YTEEIVPRHPCLKLISNCEQMLSSHTSRRLITME 421

Query: 146 K--------RSSYTEEIAEAARLKHEEFRENH 168
           K        ++SY  +        H  FRE +
Sbjct: 422 KVKEFKDQDQNSYLSDGQYMPYKGHNSFREKY 444

[Close]

BLAST of Spg034872 vs. ExPASy Swiss-Prot
Match: Q5R962 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Pongo abelii OX=9601 GN=TRMT11 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 7.1e-17
Identity = 60/150 (40.00%), Postives = 81/150 (54.00%), Query Frame = 0


Query: 27  FDAIICDPPYGVRAGGRKSGGRK-LLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 86
           FDAII DPPYG+R   R++G +K + KG       P+     H+P + +Y L +   DLL
Sbjct: 302 FDAIITDPPYGIRESTRRTGSQKEIPKGIEKWEKCPE----SHVPVSLSYHLSDMFLDLL 361

Query: 87  DLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRVLLTMVK- 146
           + AA  LV+GGRLV++ PV   E       P HPC  LIS+ EQ LSS  SR L+TM K 
Sbjct: 362 NFAAETLVLGGRLVYWLPVYTPE-YTEEMVPWHPCLELISNCEQKLSSHTSRRLITMEKV 421

Query: 147 -----RSSYTEEIAE--AARLKHEEFRENH 168
                R  Y+  +++       H  FRE +
Sbjct: 422 KKFENRDQYSHLLSDHFLPYQGHNSFREKY 446

[Close]

BLAST of Spg034872 vs. ExPASy Swiss-Prot
Match: Q7Z4G4 (tRNA (guanine(10)-N2)-methyltransferase homolog OS=Homo sapiens OX=9606 GN=TRMT11 PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 9.2e-17
Identity = 59/150 (39.33%), Postives = 81/150 (54.00%), Query Frame = 0


Query: 27  FDAIICDPPYGVRAGGRKSGGRK-LLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 86
           FDAII DPPYG+R   R++G +K + KG       P+     H+P + +Y L +   DLL
Sbjct: 302 FDAIITDPPYGIRESTRRTGSQKEIPKGIEKWEKCPE----SHVPVSLSYHLSDMFLDLL 361

Query: 87  DLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRVLLTMVK- 146
           + AA  LV+GGRLV++ PV   E       P HPC  L+S+ EQ LSS  SR L+TM K 
Sbjct: 362 NFAAETLVLGGRLVYWLPVYTPE-YTEEMVPWHPCLELVSNCEQKLSSHTSRRLITMEKV 421

Query: 147 -----RSSYTEEIAE--AARLKHEEFRENH 168
                R  Y+  +++       H  FRE +
Sbjct: 422 KKFENRDQYSHLLSDHFLPYQGHNSFREKY 446

[Close]

BLAST of Spg034872 vs. ExPASy TrEMBL
Match: A0A1S3BG98 (tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucumis melo OX=3656 GN=LOC103489693 PE=4 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.7e-96
Identity = 174/192 (90.62%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 19  WEVSMPYIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 78
           W   +  IFDAIICDPPYGVRAGGRKSGGRKLLKG VAPYTVPDDKRTDHIPSTA+YSLV
Sbjct: 289 WRPGLKEIFDAIICDPPYGVRAGGRKSGGRKLLKGIVAPYTVPDDKRTDHIPSTASYSLV 348

Query: 79  ECVHDLLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRV 138
           ECVHDLLDLAA+MLVMGGRLVFFYP+LREEDCVFNQFPEHPCF LISSSEQILSSRYSRV
Sbjct: 349 ECVHDLLDLAAKMLVMGGRLVFFYPMLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRV 408

Query: 139 LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPATDPDLAVSGNGNVS 198
           LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSP+ +  L V+GNGNVS
Sbjct: 409 LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSVNRQLGVAGNGNVS 468

Query: 199 KDPKPKYRGKYV 211
           KDPKPKYRGKYV
Sbjct: 469 KDPKPKYRGKYV 480

[Close]

BLAST of Spg034872 vs. ExPASy TrEMBL
Match: A0A6J1F3J0 (tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucurbita moschata OX=3662 GN=LOC111439552 PE=4 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 7.9e-96
Identity = 173/192 (90.10%), Postives = 180/192 (93.75%), Query Frame = 0


Query: 19  WEVSMPYIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 78
           W   +  IFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV
Sbjct: 289 WRPGLKEIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 348

Query: 79  ECVHDLLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRV 138
           ECVHDLL+LAA+MLVMGGRLVFFYPVLREEDCVFNQFPEHPCF LISSSEQILSSRYSRV
Sbjct: 349 ECVHDLLNLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRV 408

Query: 139 LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPATDPDLAVSGNGNVS 198
           LLTMVK SSYTEE+AEAA+LKHEEFRENHLKWLEDGNLHSAVFSP  DP LAV+GN N +
Sbjct: 409 LLTMVKHSSYTEEMAEAAKLKHEEFRENHLKWLEDGNLHSAVFSPTIDPQLAVAGNCNAT 468

Query: 199 KDPKPKYRGKYV 211
           KDPKPKYRGKYV
Sbjct: 469 KDPKPKYRGKYV 480

[Close]

BLAST of Spg034872 vs. ExPASy TrEMBL
Match: A0A0A0K3X0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G229890 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.0e-95
Identity = 172/185 (92.97%), Postives = 179/185 (96.76%), Query Frame = 0


Query: 26  IFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLVECVHDLL 85
           IFDAIICDPPYGVRAGGRKSGGRKLLKG VAPYTVPDDKRTDHIPSTA+YSLVECVHDLL
Sbjct: 42  IFDAIICDPPYGVRAGGRKSGGRKLLKGIVAPYTVPDDKRTDHIPSTASYSLVECVHDLL 101

Query: 86  DLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRVLLTMVKR 145
           DLAA+MLVMGGRLVFFYP+LREEDCVFNQFPEHPCF  ISSSEQILSSRYSRVLLTMVKR
Sbjct: 102 DLAAKMLVMGGRLVFFYPMLREEDCVFNQFPEHPCFTSISSSEQILSSRYSRVLLTMVKR 161

Query: 146 SSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPATDPDLAVSGNGNVSKDPKPKY 205
           SSYTEE+AEAARLKHEEFRENHLKWLEDGNLHSAVFSP+ +P L V+GNGNVSKDPKPKY
Sbjct: 162 SSYTEEMAEAARLKHEEFRENHLKWLEDGNLHSAVFSPSVNPQLGVAGNGNVSKDPKPKY 221

Query: 206 RGKYV 211
           RGKYV
Sbjct: 222 RGKYV 226

[Close]

BLAST of Spg034872 vs. ExPASy TrEMBL
Match: A0A6J1D5Q5 (tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Momordica charantia OX=3673 GN=LOC111017553 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.0e-95
Identity = 173/192 (90.10%), Postives = 181/192 (94.27%), Query Frame = 0


Query: 19  WEVSMPYIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 78
           W   +  IFDAIICDPPYGVRAGGRKSGGRKLLKG VAPYT PDDKRTDHIPSTAAYSLV
Sbjct: 292 WRPGLKEIFDAIICDPPYGVRAGGRKSGGRKLLKGIVAPYTXPDDKRTDHIPSTAAYSLV 351

Query: 79  ECVHDLLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRV 138
           ECVHDLLDLAARMLVMGGRLVFFYPVLREEDC FNQFPEHPCF LISSSEQILSSRYSRV
Sbjct: 352 ECVHDLLDLAARMLVMGGRLVFFYPVLREEDCAFNQFPEHPCFTLISSSEQILSSRYSRV 411

Query: 139 LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPATDPDLAVSGNGNVS 198
           LLTMVKR+SYTEE+AEA+RLKHEEFRENHLKWLEDGNLHSAVFSPA DP+LAV+GNG+V 
Sbjct: 412 LLTMVKRNSYTEELAEASRLKHEEFRENHLKWLEDGNLHSAVFSPA-DPELAVAGNGSVG 471

Query: 199 KDPKPKYRGKYV 211
           +DPKPKYRGKYV
Sbjct: 472 RDPKPKYRGKYV 482

[Close]

BLAST of Spg034872 vs. ExPASy TrEMBL
Match: A0A6J1ICG5 (tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472466 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.3e-94
Identity = 170/192 (88.54%), Postives = 178/192 (92.71%), Query Frame = 0


Query: 19  WEVSMPYIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 78
           W   +  IFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV
Sbjct: 289 WRPGLKEIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 348

Query: 79  ECVHDLLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRV 138
           ECVHDLL+LAA+MLVMGGRLVFFYPVLREEDCVFNQFPEHPCF LISSSEQILSSRYSRV
Sbjct: 349 ECVHDLLNLAAKMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFTLISSSEQILSSRYSRV 408

Query: 139 LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPATDPDLAVSGNGNVS 198
           LLTMVK SSYT+E+AEAA+LKHEEFRENHLKWLEDGNLHSAVF P  DP L V+GN N +
Sbjct: 409 LLTMVKHSSYTKEMAEAAKLKHEEFRENHLKWLEDGNLHSAVFGPTIDPRLTVAGNCNAT 468

Query: 199 KDPKPKYRGKYV 211
           KDPKPKYRGKYV
Sbjct: 469 KDPKPKYRGKYV 480

[Close]

BLAST of Spg034872 vs. TAIR 10
Match: AT3G26410.1 (methyltransferases;nucleic acid binding )

HSP 1 Score: 288.5 bits (737), Expect = 4.3e-78
Identity = 141/192 (73.44%), Postives = 156/192 (81.25%), Query Frame = 0


Query: 19  WEVSMPYIFDAIICDPPYGVRAGGRKSGGRKLLKGTVAPYTVPDDKRTDHIPSTAAYSLV 78
           W   +  IFDAIICDPPYGVRAGGRKSGGRK+L+GTV PYTVPDDKRTDHIPST AYSLV
Sbjct: 288 WRSGLKEIFDAIICDPPYGVRAGGRKSGGRKILRGTVDPYTVPDDKRTDHIPSTGAYSLV 347

Query: 79  ECVHDLLDLAARMLVMGGRLVFFYPVLREEDCVFNQFPEHPCFALISSSEQILSSRYSRV 138
           ECVHDLL LAARMLVM GRLVFF+PVLR+E     +FPEHPCF L++ SEQILSSRYSRV
Sbjct: 348 ECVHDLLHLAARMLVMKGRLVFFFPVLRDETGSEVKFPEHPCFKLVAVSEQILSSRYSRV 407

Query: 139 LLTMVKRSSYTEEIAEAARLKHEEFRENHLKWLEDGNLHSAVFSPATDPDLAVSGNGNVS 198
           LLTMVK   Y+EE+ EAARL H EFRENHLKWLEDGN+HS++F P       +  +    
Sbjct: 408 LLTMVKVEPYSEEVEEAARLMHLEFRENHLKWLEDGNIHSSIFKPIDSSQ--IDTDSKAF 467

Query: 199 KDPKPKYRGKYV 211
           KDPKPKYRGKYV
Sbjct: 468 KDPKPKYRGKYV 477

The following BLAST results are available for this feature:

BLAST of Spg034872 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888658.1	6.7e-97	91.67	tRNA (guanine(10)-N2)-methyltransferase homolog [Benincasa hispida]	[more]
XP_031744574.1	5.6e-96	90.10	tRNA (guanine(10)-N2)-methyltransferase homolog [Cucumis sativus]	[more]
XP_008447182.1	5.6e-96	90.63	PREDICTED: tRNA (guanine(10)-N2)-methyltransferase homolog [Cucumis melo]	[more]
XP_022932935.1	1.6e-95	90.10	tRNA (guanine(10)-N2)-methyltransferase homolog [Cucurbita moschata] >XP_0235116...	[more]
KAG7011460.1	2.8e-95	89.58	tRNA (guanine(10)-N2)-methyltransferase-like protein [Cucurbita argyrosperma sub...	[more]

BLAST of Spg034872 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54QA6	8.1e-21	42.07	tRNA (guanine(10)-N2)-methyltransferase homolog OS=Dictyostelium discoideum OX=4...	[more]
Q6NS23	4.9e-18	39.10	tRNA (guanine(10)-N2)-methyltransferase homolog OS=Xenopus laevis OX=8355 GN=trm...	[more]
Q6YJI5	1.1e-17	39.47	tRNA (guanine(10)-N2)-methyltransferase homolog OS=Gallus gallus OX=9031 GN=TRMT...	[more]
Q5R962	7.1e-17	40.00	tRNA (guanine(10)-N2)-methyltransferase homolog OS=Pongo abelii OX=9601 GN=TRMT1...	[more]
Q7Z4G4	9.2e-17	39.33	tRNA (guanine(10)-N2)-methyltransferase homolog OS=Homo sapiens OX=9606 GN=TRMT1...	[more]

BLAST of Spg034872 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BG98	2.7e-96	90.63	tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1F3J0	7.9e-96	90.10	tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucurbita moschata OX=3662 GN...	[more]
A0A0A0K3X0	3.0e-95	92.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G229890 PE=4 SV=1	[more]
A0A6J1D5Q5	3.0e-95	90.10	tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Momordica charantia OX=3673 G...	[more]
A0A6J1ICG5	3.3e-94	88.54	tRNA (Guanine(10)-N2)-methyltransferase homolog OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of Spg034872 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26410.1	4.3e-78	73.44	methyltransferases;nucleic acid binding	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 7..117 e-value: 8.4E-7 score: 30.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 191..210
None	No IPR available	PANTHER	PTHR13370	RNA METHYLASE-RELATED	coord: 17..192
None	No IPR available	PANTHER	PTHR13370:SF19	SUBFAMILY NOT NAMED	coord: 17..192
IPR002052	DNA methylase, N-6 adenine-specific, conserved site	PROSITE	PS00092	N6_MTASE	coord: 30..36
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 25..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg034872.1	Spg034872.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0032259	methylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004809	tRNA (guanine-N2-)-methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity