Spg034826 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg034826
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold3: 25647586 .. 25649045 (+)
RNA-Seq Expression	Spg034826
Synteny	Spg034826

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTATCACAACGTCGATCCTCTTCTTCTTCTCTTTGTTCATTCAAACATCAAGTGCAGCCAATTATAATGTGCTTAGTTTTGGTGCCAAATCCGATGGAAAAACCGACTCGACCGAACCATTTCTCAAAGCATGGACATTAGCTTGTAGCTCCTCCACACCATCCACTATCAACGTCCCTAAAGGAAGGTTCTTGCTTACTCCCATCACATTTAGAGGTCCGTGTAAGAACAAAATTACATTTCGCTTAAATGGAACGCTGGTCGCACCTCTAGATTATCGTGCCCTTGGAAATTCTGGGTATTGGATTTTGTTCATCAAAGTTGATGGAGTTTCTGTTATTGGTGGAAACATTGATGCAAAGGGTGCTGGATATTGGGCTTGCAAAAAATTTGGAAGAAATTGCCCAGTTGGAGCTAGGGTGAGTAATTAACATATATACTTAGACTTTATACAATTTCTTTTTGTTTGGTTTGGATCCATTTATATGTATGTCCTAATGGTCTCTCTTCGTATTAATGAAATGCAGTCAATGACATTTAATTGGGCAAACAATATCCTGGTTAGTGGATTGACCTCGGTCAACAGTCAACAGACTCATCTTGTGATCAATGGTTGCAACAATGTATGGGTTAAAAATGTGAGGGCGATGGCTCCAGATCAAAGCCCTAATACAGATGGCATTCACGTTCAATCATCTACTGGTGTTATGATCAGTGGAAGCACCCTCCAAACAGGAGATGATTGCATATCCATTGGAGATGGAACTAAAAATTTGTTTATGAGTAACATCAAATGTGGGCCTGGACATGGTGTAAGGTAATGTGTATATAGAAAGACAGTTTACCATATTTATATATTGGTGTTTAATTTTGAGGTAAAACAATATGATCTAACTTCAATGAATGCATGCAGTATTGGTAGTTTGGGAAAAGAGTTTAATGAAGATGGAGTCGAGAATGTAACTTTGACAAATGCAGTGTTTATTGGATCTGATAATGGTGTGAGGATAAAGACATGGCCGACACCTAGCAAAGGTTTTGTGAGAAACGTTCTCTTTCAAAACATCATTATCAACAATGTTAAGAATCCCATCTTAATCGATCAAAACTATTGTCCAAACAAAGGTTGTCCTTCCAAGGTAATTATTAAAATCTGGTCCTACACTCTCTTTGTGGAATTATATTAAATCTAAATAAACTAAATTGGTTTCTTTCGGGTTTTTTTTTTTTTAATGTTTTAATAAAGAGCTCTGGAGTAAAGATTACTAAAGTGACATATAGAAATATTAAAGGCACGTCAGCAACACCAGAAGCTATGACATTTAATTGTAGTCCCAGCAACCCATGCAGTGACGTACGATTACAAGACATCAAGCTTACATATAAGAACAAGGTAGCAGCATCCTCATGCAAGAACATTGGTGGATCAACTATGGGAGTTGAGAGTTGCCTATGA

mRNA sequence

ATGGCTATCACAACGTCGATCCTCTTCTTCTTCTCTTTGTTCATTCAAACATCAAGTGCAGCCAATTATAATGTGCTTAGTTTTGGTGCCAAATCCGATGGAAAAACCGACTCGACCGAACCATTTCTCAAAGCATGGACATTAGCTTGTAGCTCCTCCACACCATCCACTATCAACGTCCCTAAAGGAAGGTTCTTGCTTACTCCCATCACATTTAGAGGTCCGTGTAAGAACAAAATTACATTTCGCTTAAATGGAACGCTGGTCGCACCTCTAGATTATCGTGCCCTTGGAAATTCTGGGTATTGGATTTTGTTCATCAAAGTTGATGGAGTTTCTGTTATTGGTGGAAACATTGATGCAAAGGGTGCTGGATATTGGGCTTGCAAAAAATTTGGAAGAAATTGCCCAGTTGGAGCTAGGTCAATGACATTTAATTGGGCAAACAATATCCTGGTTAGTGGATTGACCTCGGTCAACAGTCAACAGACTCATCTTGTGATCAATGGTTGCAACAATGTATGGGTTAAAAATGTGAGGGCGATGGCTCCAGATCAAAGCCCTAATACAGATGGCATTCACGTTCAATCATCTACTGGTGTTATGATCAGTGGAAGCACCCTCCAAACAGGAGATGATTGCATATCCATTGGAGATGGAACTAAAAATTTGTTTATGAGTAACATCAAATGTGGGCCTGGACATGGTGTAAGTATTGGTAGTTTGGGAAAAGAGTTTAATGAAGATGGAGTCGAGAATGTAACTTTGACAAATGCAGTGTTTATTGGATCTGATAATGGTGTGAGGATAAAGACATGGCCGACACCTAGCAAAGGCACGTCAGCAACACCAGAAGCTATGACATTTAATTGTAGTCCCAGCAACCCATGCAGTGACGTACGATTACAAGACATCAAGCTTACATATAAGAACAAGGTAGCAGCATCCTCATGCAAGAACATTGGTGGATCAACTATGGGAGTTGAGAGTTGCCTATGA

Coding sequence (CDS)

ATGGCTATCACAACGTCGATCCTCTTCTTCTTCTCTTTGTTCATTCAAACATCAAGTGCAGCCAATTATAATGTGCTTAGTTTTGGTGCCAAATCCGATGGAAAAACCGACTCGACCGAACCATTTCTCAAAGCATGGACATTAGCTTGTAGCTCCTCCACACCATCCACTATCAACGTCCCTAAAGGAAGGTTCTTGCTTACTCCCATCACATTTAGAGGTCCGTGTAAGAACAAAATTACATTTCGCTTAAATGGAACGCTGGTCGCACCTCTAGATTATCGTGCCCTTGGAAATTCTGGGTATTGGATTTTGTTCATCAAAGTTGATGGAGTTTCTGTTATTGGTGGAAACATTGATGCAAAGGGTGCTGGATATTGGGCTTGCAAAAAATTTGGAAGAAATTGCCCAGTTGGAGCTAGGTCAATGACATTTAATTGGGCAAACAATATCCTGGTTAGTGGATTGACCTCGGTCAACAGTCAACAGACTCATCTTGTGATCAATGGTTGCAACAATGTATGGGTTAAAAATGTGAGGGCGATGGCTCCAGATCAAAGCCCTAATACAGATGGCATTCACGTTCAATCATCTACTGGTGTTATGATCAGTGGAAGCACCCTCCAAACAGGAGATGATTGCATATCCATTGGAGATGGAACTAAAAATTTGTTTATGAGTAACATCAAATGTGGGCCTGGACATGGTGTAAGTATTGGTAGTTTGGGAAAAGAGTTTAATGAAGATGGAGTCGAGAATGTAACTTTGACAAATGCAGTGTTTATTGGATCTGATAATGGTGTGAGGATAAAGACATGGCCGACACCTAGCAAAGGCACGTCAGCAACACCAGAAGCTATGACATTTAATTGTAGTCCCAGCAACCCATGCAGTGACGTACGATTACAAGACATCAAGCTTACATATAAGAACAAGGTAGCAGCATCCTCATGCAAGAACATTGGTGGATCAACTATGGGAGTTGAGAGTTGCCTATGA

Protein sequence

MAITTSILFFFSLFIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSKGTSATPEAMTFNCSPSNPCSDVRLQDIKLTYKNKVAASSCKNIGGSTMGVESCL

Homology

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BLAST of Spg034826 vs. NCBI nr
Match: XP_022156860.1 (polygalacturonase-like [Momordica charantia])

HSP 1 Score: 549.7 bits (1415), Expect = 1.8e-152
Identity = 279/378 (73.81%), Postives = 297/378 (78.57%), Query Frame = 0


Query: 9   FFFSLFIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVPKGRFLLT 68
           FFF LF Q SSA +YNVLSFGAK DGKTDST+PFLKAW  AC+S T STI VPKGRFLLT
Sbjct: 11  FFFHLFTQRSSAGSYNVLSFGAKPDGKTDSTQPFLKAWKSACTSPTASTIIVPKGRFLLT 70

Query: 69  PITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDAKGAGYWA 128
             TFRGPC + ITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSV+GGNIDAKGAGYWA
Sbjct: 71  STTFRGPCNSHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVVGGNIDAKGAGYWA 130

Query: 129 CKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRAMAPDQSP 188
           CKK G NCP GARSMTFNWANNI++SGLTS+NSQQTHLVIN CNNV VKNV+ MAPDQSP
Sbjct: 131 CKKSGNNCPAGARSMTFNWANNIVISGLTSINSQQTHLVINSCNNVKVKNVKVMAPDQSP 190

Query: 189 NTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEFNE 248
           NTDGIHVQSST VMI+GSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKE  E
Sbjct: 191 NTDGIHVQSSTNVMITGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKELEE 250

Query: 249 DGVENVTLTNAVFIGSDNGVRIKTWPTPS------------------------------- 308
           +GVENVTLTNAVFIGSDNGVRIKTWP PS                               
Sbjct: 251 NGVENVTLTNAVFIGSDNGVRIKTWPRPSKGFVKNVLFQNIIMNNVQNPIIIDQNYCPNN 310

Query: 309 --------------------KGTSATPEAMTFNCSPSNPCSDVRLQDIKLTYKNKVAASS 333
                               KGTSATPEA+TF+CSP+ PCSD+RL+DIKLTYKNK A SS
Sbjct: 311 QGCPLLSSGLKISGVSYKNIKGTSATPEAVTFDCSPTTPCSDIRLEDIKLTYKNKAATSS 370

[Close]

BLAST of Spg034826 vs. NCBI nr
Match: XP_038888873.1 (polygalacturonase-like [Benincasa hispida] >XP_038888876.1 polygalacturonase-like [Benincasa hispida])

HSP 1 Score: 548.5 bits (1412), Expect = 3.9e-152
Identity = 283/391 (72.38%), Postives = 304/391 (77.75%), Query Frame = 0


Query: 1   MAITTSILFFFSLF-----IQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTP 60
           MA T+SI FFF  F     IQTSSA ++NV+SFGAK DGKTDST+ FLKAWT ACSSSTP
Sbjct: 1   MANTSSITFFFFFFFLYLLIQTSSAGSFNVISFGAKGDGKTDSTKSFLKAWTSACSSSTP 60

Query: 61  STINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVI 120
           STINVPKGRFLLTPITFRGPC N ITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVS  
Sbjct: 61  STINVPKGRFLLTPITFRGPCNNHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSFF 120

Query: 121 GGNIDAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVW 180
           GGNIDAKGAGYWACKK G+ CPVGARS+TFNWANNI VSG+TS+NSQQTHLVIN C NV 
Sbjct: 121 GGNIDAKGAGYWACKKAGKQCPVGARSLTFNWANNIYVSGITSINSQQTHLVINSCKNVM 180

Query: 181 VKNVRAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGH 240
           VKNV+ MAPDQSPNTDGIHVQSS  V I+GSTLQTGDDCISIGDGTK+L MS++KCGPGH
Sbjct: 181 VKNVKVMAPDQSPNTDGIHVQSSFNVTITGSTLQTGDDCISIGDGTKSLLMSHVKCGPGH 240

Query: 241 GVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPS------------------ 300
           GVSIGSLGKEFNEDGVENVTLTNA+FIGSDNGVRIKTWPT S                  
Sbjct: 241 GVSIGSLGKEFNEDGVENVTLTNAIFIGSDNGVRIKTWPTASNGFVKNVLFQNIIMKNVK 300

Query: 301 ---------------------------------KGTSATPEAMTFNCSPSNPCSDVRLQD 333
                                            +GTSAT EAMTF+CSPSNPCSD++LQD
Sbjct: 301 NPILIDQNYCPNNKGCPSQSSGVKISGVTYRNIQGTSATIEAMTFDCSPSNPCSDIQLQD 360

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BLAST of Spg034826 vs. NCBI nr
Match: XP_004142576.2 (polygalacturonase [Cucumis sativus] >XP_031745382.1 polygalacturonase-like [Cucumis sativus] >KGN66774.1 hypothetical protein Csa_007167 [Cucumis sativus])

HSP 1 Score: 523.9 bits (1348), Expect = 1.0e-144
Identity = 268/384 (69.79%), Postives = 297/384 (77.34%), Query Frame = 0


Query: 4   TTSILF--FFSLFIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVP 63
           T S++F  FF LFIQ S+AANYNV+SFGAK DGKTDST+ FLKAWT ACSSST STINVP
Sbjct: 5   TKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVP 64

Query: 64  KGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDA 123
           KGRFLLTPITFRGPCKNKITF LNGTLVAPLDYRALG+SGYWILFIKVDGVS +GG ID 
Sbjct: 65  KGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGTIDG 124

Query: 124 KGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRA 183
           KG  YWACK   +NCP GARS+TFNWANNI++SGLTS+NSQQTH+VIN CNNV VKNV+ 
Sbjct: 125 KGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKI 184

Query: 184 MAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGS 243
           MAPDQSPNTDGIHVQSST V I+GST++TGDDCISIGDGTKNLFMS+IKCGPGHGVSIGS
Sbjct: 185 MAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMSDIKCGPGHGVSIGS 244

Query: 244 LGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPS------------------------ 303
           LGKE NE+GVENVTL NAVF  SDNGVRIKTWPTPS                        
Sbjct: 245 LGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILID 304

Query: 304 ---------------------------KGTSATPEAMTFNCSPSNPCSDVRLQDIKLTYK 332
                                      +GTSAT EA+TF+CS SNPCSD++L+DIKLTYK
Sbjct: 305 QNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSNPCSDIKLEDIKLTYK 364

[Close]

BLAST of Spg034826 vs. NCBI nr
Match: XP_031736043.1 (polygalacturonase-like [Cucumis sativus])

HSP 1 Score: 521.5 bits (1342), Expect = 5.1e-144
Identity = 267/384 (69.53%), Postives = 297/384 (77.34%), Query Frame = 0


Query: 4   TTSILF--FFSLFIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVP 63
           T S++F  FF LFIQ S+AANYNV+SFGAK DGKTDST+ FLKAWT ACSSST STINVP
Sbjct: 5   TKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVP 64

Query: 64  KGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDA 123
           KGRFLLTPITFRGPCKNKITF LNGTLVAPLDYRALG+SGYWILFIKVDGVS +GG ID 
Sbjct: 65  KGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGTIDG 124

Query: 124 KGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRA 183
           KG  YWACK   +NCP GARS+TFNWANNI++SGLTS+NSQQTH+VIN CNNV VKNV+ 
Sbjct: 125 KGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKI 184

Query: 184 MAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGS 243
           MAPDQSPNTDGIHVQSST V I+GST++TGDDCISIGDGTKNLFMS+IKCGPGHGVSIGS
Sbjct: 185 MAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMSDIKCGPGHGVSIGS 244

Query: 244 LGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPS------------------------ 303
           LGKE NE+GVENVTL NAVF  SDNGVRIKTWPTPS                        
Sbjct: 245 LGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILID 304

Query: 304 ---------------------------KGTSATPEAMTFNCSPSNPCSDVRLQDIKLTYK 332
                                      +GTSAT EA+TF+CS S+PCSD++L+DIKLTYK
Sbjct: 305 QNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIKLTYK 364

[Close]

BLAST of Spg034826 vs. NCBI nr
Match: XP_038876456.1 (polygalacturonase-like [Benincasa hispida])

HSP 1 Score: 518.1 bits (1333), Expect = 5.7e-143
Identity = 259/373 (69.44%), Postives = 285/373 (76.41%), Query Frame = 0


Query: 7   ILFFFSLFIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVPKGRFL 66
           ++ FF  FIQ SSA +YNV++ GAK DGKTDSTE F+KAW  ACSS TPS INVPKGRFL
Sbjct: 9   VIIFFHFFIQLSSAGSYNVITLGAKPDGKTDSTESFMKAWKSACSSLTPSIINVPKGRFL 68

Query: 67  LTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDAKGAGY 126
           LTPITFRGPCKNKITFRLNGTLVAPLDYR LGNSGYWILF+KVDGVS IGGNID K   Y
Sbjct: 69  LTPITFRGPCKNKITFRLNGTLVAPLDYRGLGNSGYWILFVKVDGVSFIGGNIDGKATEY 128

Query: 127 WACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRAMAPDQ 186
           W CK  G+NCP GARS+TFNWANNI++SGLTS+NSQQ+H+VIN CNNV VKNV+ MAPDQ
Sbjct: 129 WTCKNSGKNCPPGARSITFNWANNIIISGLTSINSQQSHVVINSCNNVVVKNVKIMAPDQ 188

Query: 187 SPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEF 246
           SPNTDGIHVQSST V I GST++TGDDCISIGDGTKNLFM+NIKCGPGHGVSIGSLGKE 
Sbjct: 189 SPNTDGIHVQSSTNVTIIGSTIKTGDDCISIGDGTKNLFMTNIKCGPGHGVSIGSLGKEV 248

Query: 247 NEDGVENVTLTNAVFIGSDNGVRIKTWPTPS----------------------------- 306
           NE+GVENVTL NAVF  SDNGVRIKTWPTPS                             
Sbjct: 249 NENGVENVTLMNAVFTKSDNGVRIKTWPTPSNGFVKHVLFQNIIMNNVQNPILIDQNYCP 308

Query: 307 ----------------------KGTSATPEAMTFNCSPSNPCSDVRLQDIKLTYKNKVAA 329
                                 KGTSAT +A+TF+CS SNPCSD+RLQDIKLTYKNK A 
Sbjct: 309 NNQGCPLQSSGVKISDVTYKNIKGTSATSKAITFDCSSSNPCSDIRLQDIKLTYKNKAAT 368

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BLAST of Spg034826 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.8e-91
Identity = 180/383 (47.00%), Postives = 229/383 (59.79%), Query Frame = 0


Query: 6   SILFFFSLFIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVPKGRF 65
           S++F F +    +S   YNV S GAK+DGKTDST+ FL AW  AC+S  P  I VP G F
Sbjct: 11  SLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPAGTF 70

Query: 66  LLTPITFRGPCKNK-ITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDAKGA 125
            L  + F GPCKN  ITFR+ GTLVAP DYR +GN+  WI F  V+GV++ GG +D +G 
Sbjct: 71  FLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGILDGQGT 130

Query: 126 GYWACKK-FGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRAMA 185
             WACK   G +CP GA ++ F+ +NNI+VSGL S+NSQ  H+VIN   NV ++ VR   
Sbjct: 131 ALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQGVRVSR 190

Query: 186 PDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLG 245
              SPNTDGIHVQ S+GV I  S + TGDDC+SIG GT NL++  + CGPGHG+SIGSLG
Sbjct: 191 SGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGPGHGISIGSLG 250

Query: 246 KEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPS-------------------------- 305
           KE  E GV+NVT+    F G+ NG+RIK+W  PS                          
Sbjct: 251 KEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVNVENPIVIDQH 310

Query: 306 -------------------------KGTSATPEAMTFNCSPSNPCSDVRLQDIKLTYKNK 333
                                     GTSAT  A+ F+CSP +PC +++L+D+KLTYKN+
Sbjct: 311 YCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVKLTYKNQ 370

[Close]

BLAST of Spg034826 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 4.6e-79
Identity = 159/358 (44.41%), Postives = 215/358 (60.06%), Query Frame = 0


Query: 24  NVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVPKGRFLLTPITFRG-PCKN-KIT 83
           NVLS+GAK DG  DST+ FL AW +AC+S+ P+TI VPKGRFL+  + F G  CK   I+
Sbjct: 34  NVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQAPIS 93

Query: 84  FRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDAKGAGYWACK-KFGRNCPVGA 143
            R+ G++VAP D+R + +S +WI F  V  VS+ GG +DA+G   W CK   G NCP GA
Sbjct: 94  IRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCPTGA 153

Query: 144 RSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRAMAPDQSPNTDGIHVQSSTG 203
           +S+ F+ +NNI +SGLTS+NSQ+ H+VI+  NNV +  V+  A + SPNTDGIHV+SS  
Sbjct: 154 KSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVESSHS 213

Query: 204 VMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEFNEDGVENVTLTNAV 263
           V I+ S + TGDDCISIG G+ N+F+  I+CGPGHG+SIGSLG+   E GV+NVT++N  
Sbjct: 214 VHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAEEEQGVDNVTVSNVD 273

Query: 264 FIGSDNGVRIKTW-----------------------------------PTPSK------- 323
           F+G++NGVRIKTW                                   P P +       
Sbjct: 274 FMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYCLHKPCPKQESGVKVS 333

Query: 324 --------GTSATPEAMTFNCSPSNPCSDVRLQDIKLTYKNKVAASSCKNIGGSTMGV 329
                   GTS T  A+  +CS   PC+ + + D+ L   ++ A +SC N  GS   V
Sbjct: 334 NVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSVHRPAQASCDNANGSANDV 391

[Close]

BLAST of Spg034826 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 6.1e-63
Identity = 147/386 (38.08%), Postives = 202/386 (52.33%), Query Frame = 0


Query: 3   ITTSILFFFSLFIQTSSA---ANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTIN 62
           I  S+     LFI  S     A   V  FGAK+DGKTD ++PFL AW  AC+S TPST+ 
Sbjct: 7   IVPSMFVLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVV 66

Query: 63  VPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNI 122
           +PKG +LL+ +   GPCK  I   + GT+ AP D  A  +   W+ F  V+   + GG I
Sbjct: 67  IPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPN-WVRFYSVENFKMFGGGI 126

Query: 123 -DAKGAGYW---AC--KKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNN 182
            D +G+  +    C  ++F    PV  R   F++  N L+  +TS +S+  H+ +  C N
Sbjct: 127 FDGQGSIAYEKNTCENREFRSKLPVNIR---FDFVTNALIQDITSKDSKLFHINVFACKN 186

Query: 183 VWVKNVRAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGP 242
           + ++ ++  APD+SPNTDGIH+  S GV I  S ++TGDDCISIGDGTKN+ +  I CGP
Sbjct: 187 ITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGP 246

Query: 243 GHGVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPS---------------- 302
           GHG+SIGSLGK  NE+ VE + ++N     + NG RIKTWP                   
Sbjct: 247 GHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNN 306

Query: 303 -----------------------------------KGTSATPEAMTFNCSPSNPCSDVRL 328
                                              +GTSA PEA+ F CS S+PC +V L
Sbjct: 307 VSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVEL 366

[Close]

BLAST of Spg034826 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 7.9e-63
Identity = 148/386 (38.34%), Postives = 202/386 (52.33%), Query Frame = 0


Query: 3   ITTSILFFFSLFIQTS---SAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTIN 62
           I  S+     LFI  S   S A   V  FGAK+DGKTD ++PFL AW  AC+S TPST+ 
Sbjct: 7   IVPSMFVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVV 66

Query: 63  VPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNI 122
           +PKG +LL+ +   GPCK  I   + GT+ AP D  A  +   W+ F  V+   + GG I
Sbjct: 67  IPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPN-WVRFYSVENFKMFGGGI 126

Query: 123 -DAKGAGYW---AC--KKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNN 182
            D +G+  +    C  ++F    PV  R   F++  N L+  +TS +S+  H+ +  C N
Sbjct: 127 FDGQGSIAYEKNTCENREFRSKLPVNIR---FDFLTNALIQDITSKDSKLFHINVFACKN 186

Query: 183 VWVKNVRAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGP 242
           + ++ ++  APD+SPNTDGIH+  S GV I  S ++TGDDCISIGDGTKN+ +  I CGP
Sbjct: 187 ITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGP 246

Query: 243 GHGVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPS---------------- 302
           GHG+SIGSLGK  NE+ VE + ++N     + NG RIKTWP                   
Sbjct: 247 GHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNN 306

Query: 303 -----------------------------------KGTSATPEAMTFNCSPSNPCSDVRL 328
                                              +GTSA PEA+ F CS S+PC +V L
Sbjct: 307 VSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVEL 366

[Close]

BLAST of Spg034826 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-56
Identity = 132/348 (37.93%), Postives = 181/348 (52.01%), Query Frame = 0


Query: 37  DSTEPFLKAWTLACSSSTPSTINVPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRA 96
           DST+    AW  AC+S++PSTI VPKG F +  IT  GPCK+ I  +L GTL AP D   
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 97  LGNSGYWILFIKVDGVSVIGGNI-DAKGAGYWA---CKKFGRNCPVGARSMTFNWANNIL 156
           +   G WI   K+D +++ GG + D +G   W    C K G  C   + ++      N +
Sbjct: 61  IKGLG-WINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSI 120

Query: 157 VSGLTSVNSQQTHLVINGCNNVWVKNVRAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGD 216
           +  +T+++S+  H+ + GC N+  +  +  A + S NTDGIH+  S GV I  + ++TGD
Sbjct: 121 LRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGD 180

Query: 217 DCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKT 276
           DCIS+GDG+KN+ ++NI CGPGHG+S+GSLG+  NE+ V  + + N    GS NGVRIKT
Sbjct: 181 DCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKT 240

Query: 277 WP---------------------------------------TPS------------KGTS 328
           WP                                        PS            KGTS
Sbjct: 241 WPKSEPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTS 300

[Close]

BLAST of Spg034826 vs. ExPASy TrEMBL
Match: A0A6J1DRI6 (polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111023701 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.5e-153
Identity = 279/378 (73.81%), Postives = 297/378 (78.57%), Query Frame = 0


Query: 9   FFFSLFIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVPKGRFLLT 68
           FFF LF Q SSA +YNVLSFGAK DGKTDST+PFLKAW  AC+S T STI VPKGRFLLT
Sbjct: 11  FFFHLFTQRSSAGSYNVLSFGAKPDGKTDSTQPFLKAWKSACTSPTASTIIVPKGRFLLT 70

Query: 69  PITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDAKGAGYWA 128
             TFRGPC + ITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSV+GGNIDAKGAGYWA
Sbjct: 71  STTFRGPCNSHITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVVGGNIDAKGAGYWA 130

Query: 129 CKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRAMAPDQSP 188
           CKK G NCP GARSMTFNWANNI++SGLTS+NSQQTHLVIN CNNV VKNV+ MAPDQSP
Sbjct: 131 CKKSGNNCPAGARSMTFNWANNIVISGLTSINSQQTHLVINSCNNVKVKNVKVMAPDQSP 190

Query: 189 NTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEFNE 248
           NTDGIHVQSST VMI+GSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKE  E
Sbjct: 191 NTDGIHVQSSTNVMITGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKELEE 250

Query: 249 DGVENVTLTNAVFIGSDNGVRIKTWPTPS------------------------------- 308
           +GVENVTLTNAVFIGSDNGVRIKTWP PS                               
Sbjct: 251 NGVENVTLTNAVFIGSDNGVRIKTWPRPSKGFVKNVLFQNIIMNNVQNPIIIDQNYCPNN 310

Query: 309 --------------------KGTSATPEAMTFNCSPSNPCSDVRLQDIKLTYKNKVAASS 333
                               KGTSATPEA+TF+CSP+ PCSD+RL+DIKLTYKNK A SS
Sbjct: 311 QGCPLLSSGLKISGVSYKNIKGTSATPEAVTFDCSPTTPCSDIRLEDIKLTYKNKAATSS 370

[Close]

BLAST of Spg034826 vs. ExPASy TrEMBL
Match: A0A0A0M0F3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G689640 PE=3 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 5.0e-145
Identity = 268/384 (69.79%), Postives = 297/384 (77.34%), Query Frame = 0


Query: 4   TTSILF--FFSLFIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVP 63
           T S++F  FF LFIQ S+AANYNV+SFGAK DGKTDST+ FLKAWT ACSSST STINVP
Sbjct: 5   TKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVP 64

Query: 64  KGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDA 123
           KGRFLLTPITFRGPCKNKITF LNGTLVAPLDYRALG+SGYWILFIKVDGVS +GG ID 
Sbjct: 65  KGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGTIDG 124

Query: 124 KGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRA 183
           KG  YWACK   +NCP GARS+TFNWANNI++SGLTS+NSQQTH+VIN CNNV VKNV+ 
Sbjct: 125 KGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKI 184

Query: 184 MAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGS 243
           MAPDQSPNTDGIHVQSST V I+GST++TGDDCISIGDGTKNLFMS+IKCGPGHGVSIGS
Sbjct: 185 MAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMSDIKCGPGHGVSIGS 244

Query: 244 LGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPS------------------------ 303
           LGKE NE+GVENVTL NAVF  SDNGVRIKTWPTPS                        
Sbjct: 245 LGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILID 304

Query: 304 ---------------------------KGTSATPEAMTFNCSPSNPCSDVRLQDIKLTYK 332
                                      +GTSAT EA+TF+CS SNPCSD++L+DIKLTYK
Sbjct: 305 QNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSNPCSDIKLEDIKLTYK 364

[Close]

BLAST of Spg034826 vs. ExPASy TrEMBL
Match: A0A6J1JKS6 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486681 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.6e-138
Identity = 253/380 (66.58%), Postives = 287/380 (75.53%), Query Frame = 0


Query: 7   ILFFFSLFIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVPKGRFL 66
           I+  F  FIQ SSAA YNV+++GAK DGKTDST PFLKAWT AC+SSTPSTINVPKGRFL
Sbjct: 11  IISSFYFFIQLSSAATYNVITYGAKPDGKTDSTRPFLKAWTSACTSSTPSTINVPKGRFL 70

Query: 67  LTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDAKGAGY 126
           L PITFRGPCKNKITFRLNGTLVAPLD+RALG+SGYWILFIKV+ VS  GGNIDAKGA Y
Sbjct: 71  LKPITFRGPCKNKITFRLNGTLVAPLDFRALGSSGYWILFIKVNDVSFFGGNIDAKGASY 130

Query: 127 WACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRAMAPDQ 186
           WACK  G++CP GARS+TFNWANNI +SGLTS+NS+Q+H+ IN CNNV VKNV+ MAPDQ
Sbjct: 131 WACKDSGKDCPPGARSLTFNWANNIYLSGLTSINSRQSHITINSCNNVLVKNVKIMAPDQ 190

Query: 187 SPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEF 246
           SPNTDGIHVQSST V I+G+T+QTGDDCISIGDGTKNLFM++IKCGPGHGVSIGSLGKEF
Sbjct: 191 SPNTDGIHVQSSTDVTITGTTIQTGDDCISIGDGTKNLFMAHIKCGPGHGVSIGSLGKEF 250

Query: 247 NEDGVENVTLTNAVFIGSDNGVRIKTWPTPSK---------------------------- 306
           +E GV+NVTL NAVF  SDNGVRIKTW  P                              
Sbjct: 251 DEQGVQNVTLMNAVFTKSDNGVRIKTWARPGNGFVKHVVFENIIMNNVKNPIIIDQNYCP 310

Query: 307 -----------------------GTSATPEAMTFNCSPSNPCSDVRLQDIKLTYKNKVAA 333
                                  GTSAT EAMTF+CS +NPC +VRL+DIKLTYKNK + 
Sbjct: 311 DNVGCPGQSAGVKISDVRYKNIGGTSATSEAMTFDCSSTNPCDNVRLEDIKLTYKNKAST 370

[Close]

BLAST of Spg034826 vs. ExPASy TrEMBL
Match: A0A6J1JGH0 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486682 PE=3 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 3.5e-138
Identity = 252/380 (66.32%), Postives = 288/380 (75.79%), Query Frame = 0


Query: 7   ILFFFSLFIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVPKGRFL 66
           I+  F  FIQ SSAA YNV+++GAK DGKTDST PFLKAWT AC+SSTPSTI+VPKGRFL
Sbjct: 11  IISSFYFFIQLSSAATYNVITYGAKPDGKTDSTRPFLKAWTSACTSSTPSTISVPKGRFL 70

Query: 67  LTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDAKGAGY 126
           L PITFRGPCKNKITFRLNGTLVAPLD+RALG+SGYWILFIKV+ VSV GGNIDAKGA Y
Sbjct: 71  LKPITFRGPCKNKITFRLNGTLVAPLDFRALGSSGYWILFIKVNDVSVFGGNIDAKGASY 130

Query: 127 WACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRAMAPDQ 186
           WAC+  G++CP GARS+TFNWANNI +SGLTS+NS+Q+H+ IN CNNV VKNV+ MAPDQ
Sbjct: 131 WACRDSGKDCPPGARSLTFNWANNIYLSGLTSINSRQSHITINSCNNVLVKNVKIMAPDQ 190

Query: 187 SPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGKEF 246
           SPNTDGIHVQSST V I+G+T+QTGDDCISIGDGTKNLFM++IKCGPGHGVSIGSLGKEF
Sbjct: 191 SPNTDGIHVQSSTDVTITGTTIQTGDDCISIGDGTKNLFMTHIKCGPGHGVSIGSLGKEF 250

Query: 247 NEDGVENVTLTNAVFIGSDNGVRIKTWPTPSK---------------------------- 306
           +E GV+NVTL NAVF  SDNGVRIKTW  P                              
Sbjct: 251 DEQGVQNVTLMNAVFTKSDNGVRIKTWARPGNGYVKHVVFENIVMNNVKNPIIIDQNYCP 310

Query: 307 -----------------------GTSATPEAMTFNCSPSNPCSDVRLQDIKLTYKNKVAA 333
                                  GTSAT EAMTF+CS +NPC +VRL+DIKLTYKNK + 
Sbjct: 311 DNIGCPGQSAGVKISDVRYKNIGGTSATSEAMTFDCSSTNPCDNVRLEDIKLTYKNKAST 370

[Close]

BLAST of Spg034826 vs. ExPASy TrEMBL
Match: A0A6J1EMF9 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434691 PE=3 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 3.5e-138
Identity = 254/386 (65.80%), Postives = 290/386 (75.13%), Query Frame = 0


Query: 3   ITTSILFFFSL--FIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINV 62
           I T +L   S+  F+Q SSAA YNV+++GAK +GKTDST PFLKAWT ACSSSTPSTINV
Sbjct: 5   IKTLLLIISSIYFFLQLSSAATYNVITYGAKPNGKTDSTRPFLKAWTSACSSSTPSTINV 64

Query: 63  PKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNID 122
           PKGRFLL PITFRGPC NKITFRLNGTLVAPLDYRALG+SGYWILFIKV+ VS  GGNID
Sbjct: 65  PKGRFLLKPITFRGPCNNKITFRLNGTLVAPLDYRALGSSGYWILFIKVNDVSFFGGNID 124

Query: 123 AKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVR 182
           AKGA YWAC+  G++CP GARS+TFNWANNI++SGLTS+NS+Q+H+ IN CNNV VKNV+
Sbjct: 125 AKGASYWACRDSGKDCPPGARSITFNWANNIILSGLTSLNSRQSHITINSCNNVLVKNVK 184

Query: 183 AMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIG 242
            MAPDQSPNTDGIHVQSST V I+G+T+QTGDDCISIGDGTKNLFM++IKCGPGHGVSIG
Sbjct: 185 IMAPDQSPNTDGIHVQSSTDVTITGTTIQTGDDCISIGDGTKNLFMTHIKCGPGHGVSIG 244

Query: 243 SLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSK---------------------- 302
           SLGKEF+E GV+NVTL NAVF  SDNGVRIKTW  P                        
Sbjct: 245 SLGKEFDEQGVQNVTLMNAVFTKSDNGVRIKTWARPGNGFVKHVVFQNIVMNNVKNPIII 304

Query: 303 -----------------------------GTSATPEAMTFNCSPSNPCSDVRLQDIKLTY 333
                                        GTSAT EA+TF+CSP+NPC D+RL+DIKLTY
Sbjct: 305 DQNYCPDNEGCPGQSAGVKISDVAYKNIGGTSATSEAITFDCSPTNPCGDIRLEDIKLTY 364

[Close]

BLAST of Spg034826 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 426.4 bits (1095), Expect = 2.1e-119
Identity = 210/383 (54.83%), Postives = 266/383 (69.45%), Query Frame = 0


Query: 7   ILFFFSLFI---QTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVPKG 66
           +L FFS F+    +++A+NYNV+SFGAK DG+TDST+ FL AW  AC S+   T+ VP+G
Sbjct: 10  LLMFFSSFLLMKSSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAAAVTVTVPRG 69

Query: 67  RFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDAKG 126
            FLL P+ FRGPC+++ITF++ GT+VAP DYR LGNSGYWILF+KV+ +S+IGG +DA+G
Sbjct: 70  SFLLKPVEFRGPCRSRITFQIYGTIVAPSDYRGLGNSGYWILFVKVNRISIIGGTLDARG 129

Query: 127 AGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRAMA 186
           A +WAC+K G++CPVGARSMTFNWAN+++VSGLTS+NSQ THLVIN CNNV V+ V+ +A
Sbjct: 130 ASFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLVINSCNNVIVRKVKLVA 189

Query: 187 PDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLG 246
           PDQSPNTDG+HVQ S GV ++  T  TGDDCISIG GT+NL+MS + CGPGHG+SIGSLG
Sbjct: 190 PDQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKLNCGPGHGISIGSLG 249

Query: 247 KEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPS-------------------------- 306
           ++ NE GVEN+TL N+VF GSDNGVRIKTW   S                          
Sbjct: 250 RDANEAGVENITLINSVFSGSDNGVRIKTWARQSTGFVRNVLFQNLIMKNVQNPIIVDQN 309

Query: 307 -------------------------KGTSATPEAMTFNCSPSNPCSDVRLQDIKLTYKNK 333
                                    +GTS T +A+TF+CS SNPC  +RL DIKLT+  +
Sbjct: 310 YCPSNQGCPKQGSGVKISQVVYRNIQGTSRTQQALTFDCSRSNPCQAIRLHDIKLTFNGR 369

[Close]

BLAST of Spg034826 vs. TAIR 10
Match: AT1G05650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 375.9 bits (964), Expect = 3.3e-104
Identity = 198/388 (51.03%), Postives = 252/388 (64.95%), Query Frame = 0


Query: 2   AITTSILFFFSLFIQTSSAAN--YNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTIN 61
           AIT  ++F    FI  SS+A+  +NV+SFGAK DG TDST  FLKAW  AC S+  +T+ 
Sbjct: 5   AITFPLIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASATVV 64

Query: 62  VPKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNI 121
           VP G FLL  ITF GPCK+KITF++ GT+VAP DYR  GNSG WILF KV+  S++GG  
Sbjct: 65  VPTGTFLLKVITFGGPCKSKITFQVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLVGGTF 124

Query: 122 DAKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNV 181
           DA+G+G+W+C+K G+NCP G RS++FN A ++++SG+ S+NSQ +H+ +NGC NV V+N+
Sbjct: 125 DARGSGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVSHMTLNGCTNVAVRNI 184

Query: 182 RAMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSI 241
           R +AP  SPNTDG  VQ STGV ++GST+QTGDDC++IG GT+N  +S + CGPGHGVSI
Sbjct: 185 RLVAPGDSPNTDGFTVQFSTGVTLTGSTVQTGDDCVAIGQGTRNFLISKLACGPGHGVSI 244

Query: 242 GSLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPS---------------------- 301
           GSL K+ NEDGVENVT++++VF GS NGVRIK+W  PS                      
Sbjct: 245 GSLAKQLNEDGVENVTVSSSVFTGSQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQNPII 304

Query: 302 -----------------------------KGTSATPEAMTFNCSPSNPCSDVRLQDIKLT 333
                                        +GTSAT EAM   CS SNPC+ + LQDIKLT
Sbjct: 305 IDQNYCPSNQGCPTEHSGVKITQVTYKNIQGTSATQEAMKLVCSKSNPCTGITLQDIKLT 364

[Close]

BLAST of Spg034826 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 373.2 bits (957), Expect = 2.1e-103
Identity = 199/387 (51.42%), Postives = 253/387 (65.37%), Query Frame = 0


Query: 3   ITTSILFFFSLFIQTS-SAAN-YNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINV 62
           I  S+LF    FI  S SA+N +NV+SFGAK DG TDST  FLKAW  AC S++ +T+ V
Sbjct: 6   IRFSLLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASSATVVV 65

Query: 63  PKGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNID 122
           PKG FLL  ITF GPCK+KITF++ GT++AP DYR  GNSG+WILF KV+  S++GG  D
Sbjct: 66  PKGTFLLKVITFGGPCKSKITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVGGTFD 125

Query: 123 AKGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVR 182
           A+  G+W+C+K G+NCP G RS++FN A ++++SG+ S+NSQ TH+ +NGC NV V+NV+
Sbjct: 126 ARANGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVVRNVK 185

Query: 183 AMAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIG 242
            +AP  SPNTDG HVQ STGV  +GST+QTGDDC++IG GT+NL ++ + CGPGHGVSIG
Sbjct: 186 LVAPGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPGHGVSIG 245

Query: 243 SLGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPS----------------------- 302
           SL KE  EDGVENVT++++VF GS NGVRIK+W  PS                       
Sbjct: 246 SLAKELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNVENPIII 305

Query: 303 ----------------------------KGTSATPEAMTFNCSPSNPCSDVRLQDIKLTY 333
                                       +GTSAT EAM   CS S+PC+ + LQDIKLTY
Sbjct: 306 DQNYCPTHEGCPNEYSGVKISQVTYKNIQGTSATQEAMKLVCSKSSPCTGITLQDIKLTY 365

[Close]

BLAST of Spg034826 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 359.4 bits (921), Expect = 3.2e-99
Identity = 184/373 (49.33%), Postives = 237/373 (63.54%), Query Frame = 0


Query: 7   ILFFFSLFIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVPKGRFL 66
           ++ F   FI + SA +YNVLSFGAK DGKTD+T+ F+  W  AC+SS P TI VPKGRFL
Sbjct: 5   LVLFVFFFISSCSAQSYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGRFL 64

Query: 67  LTPITFRG-PCKNK-ITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDAKGA 126
           L  +TF G  CK K +TFR++GTLVAP DYR +GN  YWI F  +DG++V GG +DA+GA
Sbjct: 65  LRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDARGA 124

Query: 127 GYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRAMAP 186
             W CKK G+NCP GA ++ F  ++N++VSGLTS+NSQ  H+VINGCNNV ++ V+ +A 
Sbjct: 125 SLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKVLAA 184

Query: 187 DQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGSLGK 246
             SPNTDGIHVQSS+ V I  + + TGDDC+SIG GT  L++ N+ CGPGHG+SIGSLGK
Sbjct: 185 GNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSLGK 244

Query: 247 EFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPSK-------------------------- 306
           +  E GV+NVT+    F G+DNGVRIK+W  PS                           
Sbjct: 245 DSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIIDQNY 304

Query: 307 ------------------------GTSATPEAMTFNCSPSNPCSDVRLQDIKLTYKNKVA 328
                                   GTSAT   +  +CS   PC+ +RL+D+KLTY+NK A
Sbjct: 305 CPDHDCPRQVSGIKISDVLFVDIHGTSATEVGVKLDCSSKKPCTGIRLEDVKLTYQNKPA 364

[Close]

BLAST of Spg034826 vs. TAIR 10
Match: AT2G43880.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 356.3 bits (913), Expect = 2.7e-98
Identity = 180/378 (47.62%), Postives = 243/378 (64.29%), Query Frame = 0


Query: 6   SILFF----FSLFIQTSSAANYNVLSFGAKSDGKTDSTEPFLKAWTLACSSSTPSTINVP 65
           +I+FF    F L   + +  ++NV  +GA+ DG+ D+T+ FL AW+LAC S   + + VP
Sbjct: 10  AIIFFSINIFFLIKSSHAMPSFNVQRYGARGDGRADATKSFLTAWSLACGSRARAMVYVP 69

Query: 66  KGRFLLTPITFRGPCKNKITFRLNGTLVAPLDYRALGNSGYWILFIKVDGVSVIGGNIDA 125
           +G +L+  + F GPCKN ITF+ +GTLVAP +Y  +GNSGYWILF KV+ +SV GG IDA
Sbjct: 70  RGTYLVKNLVFWGPCKNIITFKNDGTLVAPANYWDIGNSGYWILFAKVNRISVYGGTIDA 129

Query: 126 KGAGYWACKKFGRNCPVGARSMTFNWANNILVSGLTSVNSQQTHLVINGCNNVWVKNVRA 185
           +GAGYW+C+K G +CP GARS++F+W NN+L+SGL+S NSQ  H+ ++  +NV ++NVR 
Sbjct: 130 RGAGYWSCRKKGSHCPQGARSISFSWCNNVLLSGLSSFNSQNMHVTVHHSSNVRIENVRI 189

Query: 186 MAPDQSPNTDGIHVQSSTGVMISGSTLQTGDDCISIGDGTKNLFMSNIKCGPGHGVSIGS 245
            AP  SPNTDGIHVQSS+GV ISG T+ TGDDCI++  G++N+++  + CGPGHG+SIGS
Sbjct: 190 RAPSGSPNTDGIHVQSSSGVTISGGTIATGDDCIALSQGSRNIWIERVNCGPGHGISIGS 249

Query: 246 LGKEFNEDGVENVTLTNAVFIGSDNGVRIKTWPTPS------------------------ 305
           LG   NE+GV+NVT+T++VF  + NGVRIKTW  PS                        
Sbjct: 250 LGDYANEEGVQNVTVTSSVFTKTQNGVRIKTWARPSRGFVNNVVFRNLIMNNVENPVIID 309

Query: 306 ---------------------------KGTSATPEAMTFNCSPSNPCSDVRLQDIKLTYK 329
                                      KGTS TP AM  +CS SN C+ +RLQDIKLTY 
Sbjct: 310 QNYCPNGKGCPRQSSGVKISGVTFANIKGTSTTPIAMKLDCSGSNHCTGLRLQDIKLTYM 369

The following BLAST results are available for this feature:

BLAST of Spg034826 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156860.1	1.8e-152	73.81	polygalacturonase-like [Momordica charantia]	[more]
XP_038888873.1	3.9e-152	72.38	polygalacturonase-like [Benincasa hispida] >XP_038888876.1 polygalacturonase-lik...	[more]
XP_004142576.2	1.0e-144	69.79	polygalacturonase [Cucumis sativus] >XP_031745382.1 polygalacturonase-like [Cucu...	[more]
XP_031736043.1	5.1e-144	69.53	polygalacturonase-like [Cucumis sativus]	[more]
XP_038876456.1	5.7e-143	69.44	polygalacturonase-like [Benincasa hispida]	[more]

BLAST of Spg034826 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P48979	1.8e-91	47.00	Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1	[more]
O22818	4.6e-79	44.41	Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386...	[more]
Q39766	6.1e-63	38.08	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]
Q39786	7.9e-63	38.34	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]
P24548	1.4e-56	37.93	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]

BLAST of Spg034826 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DRI6	8.5e-153	73.81	polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111023701 PE=3 SV=1	[more]
A0A0A0M0F3	5.0e-145	69.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G689640 PE=3 SV=1	[more]
A0A6J1JKS6	1.6e-138	66.58	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486681 PE=3 SV=1	[more]
A0A6J1JGH0	3.5e-138	66.32	polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111486682 PE=3 SV=1	[more]
A0A6J1EMF9	3.5e-138	65.80	polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434691 PE=3 SV=1	[more]

BLAST of Spg034826 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G43890.1	2.1e-119	54.83	Pectin lyase-like superfamily protein	[more]
AT1G05650.1	3.3e-104	51.03	Pectin lyase-like superfamily protein	[more]
AT1G05660.1	2.1e-103	51.42	Pectin lyase-like superfamily protein	[more]
AT2G43870.1	3.2e-99	49.33	Pectin lyase-like superfamily protein	[more]
AT2G43880.1	2.7e-98	47.62	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 251..272 e-value: 16.0 score: 14.4 coord: 171..197 e-value: 86.0 score: 12.0 coord: 198..219 e-value: 200.0 score: 10.8
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 50..274 e-value: 9.0E-61 score: 205.7
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 228..241
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 12..331 e-value: 8.4E-88 score: 297.1
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 278..329
None	No IPR available	PANTHER	PTHR31375:SF31	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 4..279
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 4..279
None	No IPR available	PANTHER	PTHR31375:SF31	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 278..329
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 14..328

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg034826.1	Spg034826.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity