Spg034557 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg034557
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionGlycine--tRNA ligase
Locationscaffold4: 16245043 .. 16246473 (-)
RNA-Seq ExpressionSpg034557
SyntenySpg034557
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCTTATTTTCTAAGAAGAGAGGTGTATTTGAGGGTGTAAGTGAGAAATTTATAGTCCTGGTTTTGAAGCAATTGTTTCCTAGGTGTGTGTCTAAGGTTTGGGGTTCAGTTTCTTTTTGGTTCTTTGGGTCATTTCGTTGGATCAGAAGCTATTTTTGTCTTTTCCACTGCACCCATGGGGGATGAGGGGCAGCCTTCTAAGTTATTTTTGGCTTCTACGGCTCTCTTGCTGCAATTTTTTCCTTGGGCTATTTCGTTTGGGTTTGCTTGCTCTTTAGTTTTCTCTTGTTATTTGTGGGAGTATCCTTTTAGTTTTTTGAAGTATATTATATAGATGCTTTGTTGAAACAAACTTAATGGTTCTCAAATTAGTATTCAATTAATTTCAATTTTTTAATGTTCATAATCTTAAAAGATTTTCAACATTTCCAGGTCACGGTTGAGAGTCTGTGCTCTAAACAGGTAGAGAAAGAGGTTGAGGTCAGAGGACCCCCTGTTTCAAAAGCTTTTGATGACCAAGGAAATCCTACAAAGGTGAATGTTTTCTCATCATTTAATATTCTGGAACACAAAGAATTTCAATTTTTTTTTTCTATGATGATAGTTATTACTTGAGTAATATTGCAGAATAAGTTAAGCATTGGACTTTGTTACAGTTCTTATCAGTGTAATTTTAAAGTTTTATCTTTTATTGTCTTTTTACTTTCTCTTGCTCAGGTTGACCATGTACACTCGTATATATATATTTTATTTGAATGGAATAGATGATTCTGATTCTCTTAGACCTTTAGCATTAACATGGTATCAGAGTGAAGAGTCTTTTTTAGGGCAATTAGGATTTTGTGTCATTAGAGATTTGTGAAACTTTAGGGTTTGGTTTGGAACACCTTCTTGGAGGGTTTTGTTTTGTATCAATCTAAGAAGCACGGACGCGGACACGACGATACGTCAATTTCTAAAAAACTAGGACACGGACACGTTGAGGATACATTATATATATATTTTTTAAAAAATTTTTAGTGTATATATATATATTTAATTTTTAGTATATATATAAATTTTAAAAATTTTTTTTAGTATATATATATAATTTTTCAATTTCATCATTCCTTTTCTTTTTTTTTTTTTTTTAAATTTAAAAATACCTCTTTTATTGGGTTGGGCCTATTTGTGAGGGTTTGTCTAGGTAAGCTTAAACGAAGGTTTAAAAATAAAAAGAAAATACCGAAGAAGAAAAAGGTGTCGAGGTCAACTTCTGATGCTGACAGTGGCGACTTCTTGCAACAAGCGACGACAATGGCGAAGACGACTGGTGACGTCGCCAGAGGTGACAAAGAGAGAAGAAAAGCGTCAACGGAAATCATGAAGAAGAAGAATCGGAGCTGCTGGTGCTGTTTTCACTTTTCAGAAATCGTGAAGAAGAACTAG

mRNA sequence

ATGATCTTATTTTCTAAGAAGAGAGGTGTATTTGAGGGTGTAAGTGAGAAATTTATAGTCCTGGTTTTGAAGCAATTGTTTCCTAGGTGTGTGTCTAAGGTCACGGTTGAGAGTCTGTGCTCTAAACAGGTAGAGAAAGAGGTTGAGGTCAGAGGACCCCCTGTTTCAAAAGCTTTTGATGACCAAGGAAATCCTACAAAGAATAAGTTAAGCATTGGACTTTGTTACAGTTCTTATCAGTGTAAGCTTAAACGAAGGTTTAAAAATAAAAAGAAAATACCGAAGAAGAAAAAGGTGTCGAGGTCAACTTCTGATGCTGACAGTGGCGACTTCTTGCAACAAGCGACGACAATGGCGAAGACGACTGGTGACGTCGCCAGAGGTGACAAAGAGAGAAGAAAAGCGTCAACGGAAATCATGAAGAAGAAGAATCGGAGCTGCTGGTGCTGTTTTCACTTTTCAGAAATCGTGAAGAAGAACTAG

Coding sequence (CDS)

ATGATCTTATTTTCTAAGAAGAGAGGTGTATTTGAGGGTGTAAGTGAGAAATTTATAGTCCTGGTTTTGAAGCAATTGTTTCCTAGGTGTGTGTCTAAGGTCACGGTTGAGAGTCTGTGCTCTAAACAGGTAGAGAAAGAGGTTGAGGTCAGAGGACCCCCTGTTTCAAAAGCTTTTGATGACCAAGGAAATCCTACAAAGAATAAGTTAAGCATTGGACTTTGTTACAGTTCTTATCAGTGTAAGCTTAAACGAAGGTTTAAAAATAAAAAGAAAATACCGAAGAAGAAAAAGGTGTCGAGGTCAACTTCTGATGCTGACAGTGGCGACTTCTTGCAACAAGCGACGACAATGGCGAAGACGACTGGTGACGTCGCCAGAGGTGACAAAGAGAGAAGAAAAGCGTCAACGGAAATCATGAAGAAGAAGAATCGGAGCTGCTGGTGCTGTTTTCACTTTTCAGAAATCGTGAAGAAGAACTAG

Protein sequence

MILFSKKRGVFEGVSEKFIVLVLKQLFPRCVSKVTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTKNKLSIGLCYSSYQCKLKRRFKNKKKIPKKKKVSRSTSDADSGDFLQQATTMAKTTGDVARGDKERRKASTEIMKKKNRSCWCCFHFSEIVKKN
Homology
BLAST of Spg034557 vs. NCBI nr
Match: XP_022973889.1 (glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-08
Identity = 33/34 (97.06%), Postives = 34/34 (100.00%), Query Frame = 0

Query: 34  VTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTK 68
           VTVESLCSKQVEKE+EVRGPPVSKAFDDQGNPTK
Sbjct: 345 VTVESLCSKQVEKEIEVRGPPVSKAFDDQGNPTK 378

BLAST of Spg034557 vs. NCBI nr
Match: XP_023520987.1 (glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-08
Identity = 33/34 (97.06%), Postives = 34/34 (100.00%), Query Frame = 0

Query: 34  VTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTK 68
           VTVESLCSKQVEKE+EVRGPPVSKAFDDQGNPTK
Sbjct: 438 VTVESLCSKQVEKEIEVRGPPVSKAFDDQGNPTK 471

BLAST of Spg034557 vs. NCBI nr
Match: XP_022973888.1 (glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-08
Identity = 33/34 (97.06%), Postives = 34/34 (100.00%), Query Frame = 0

Query: 34  VTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTK 68
           VTVESLCSKQVEKE+EVRGPPVSKAFDDQGNPTK
Sbjct: 438 VTVESLCSKQVEKEIEVRGPPVSKAFDDQGNPTK 471

BLAST of Spg034557 vs. NCBI nr
Match: XP_023520988.1 (glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-08
Identity = 33/34 (97.06%), Postives = 34/34 (100.00%), Query Frame = 0

Query: 34  VTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTK 68
           VTVESLCSKQVEKE+EVRGPPVSKAFDDQGNPTK
Sbjct: 345 VTVESLCSKQVEKEIEVRGPPVSKAFDDQGNPTK 378

BLAST of Spg034557 vs. NCBI nr
Match: XP_038874261.1 (glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 70.5 bits (171), Expect = 1.5e-08
Identity = 38/57 (66.67%), Postives = 41/57 (71.93%), Query Frame = 0

Query: 34  VTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTKNKLSIGLCYSSYQCKLKRRFKNK 91
           VTVESLCSKQVEKE+EVRGPPVSKAFDDQGNPTK        YS  +  L R+   K
Sbjct: 437 VTVESLCSKQVEKEIEVRGPPVSKAFDDQGNPTKAAEGFCRRYSISRESLYRKIDGK 493

BLAST of Spg034557 vs. ExPASy Swiss-Prot
Match: Q8L785 (Glycine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EDD1 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 3.9e-07
Identity = 30/57 (52.63%), Postives = 37/57 (64.91%), Query Frame = 0

Query: 34  VTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTKNKLSIGLCYSSYQCKLKRRFKNK 91
           V V+++ SKQ+E+EVEVRGPP SKAFDD+GNPTK        Y     KL R+   K
Sbjct: 436 VLVDAMSSKQLEEEVEVRGPPASKAFDDEGNPTKAAEGFSRRYGVPLEKLYRKVSGK 492

BLAST of Spg034557 vs. ExPASy TrEMBL
Match: A0A6J1IFY6 (Glycine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111472479 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 5.6e-09
Identity = 33/34 (97.06%), Postives = 34/34 (100.00%), Query Frame = 0

Query: 34  VTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTK 68
           VTVESLCSKQVEKE+EVRGPPVSKAFDDQGNPTK
Sbjct: 345 VTVESLCSKQVEKEIEVRGPPVSKAFDDQGNPTK 378

BLAST of Spg034557 vs. ExPASy TrEMBL
Match: A0A6J1IEH3 (Glycine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111472479 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 5.6e-09
Identity = 33/34 (97.06%), Postives = 34/34 (100.00%), Query Frame = 0

Query: 34  VTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTK 68
           VTVESLCSKQVEKE+EVRGPPVSKAFDDQGNPTK
Sbjct: 438 VTVESLCSKQVEKEIEVRGPPVSKAFDDQGNPTK 471

BLAST of Spg034557 vs. ExPASy TrEMBL
Match: A0A6J1EYL5 (Glycine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111439724 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.6e-08
Identity = 32/34 (94.12%), Postives = 34/34 (100.00%), Query Frame = 0

Query: 34  VTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTK 68
           VTVESLCSKQVEKE+EVRGPPVSKAFDD+GNPTK
Sbjct: 438 VTVESLCSKQVEKEIEVRGPPVSKAFDDKGNPTK 471

BLAST of Spg034557 vs. ExPASy TrEMBL
Match: A0A6J1F3I3 (Glycine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111439724 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.6e-08
Identity = 32/34 (94.12%), Postives = 34/34 (100.00%), Query Frame = 0

Query: 34  VTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTK 68
           VTVESLCSKQVEKE+EVRGPPVSKAFDD+GNPTK
Sbjct: 345 VTVESLCSKQVEKEIEVRGPPVSKAFDDKGNPTK 378

BLAST of Spg034557 vs. ExPASy TrEMBL
Match: A0A1S4E2Q4 (Glycine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103498820 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.6e-08
Identity = 33/34 (97.06%), Postives = 33/34 (97.06%), Query Frame = 0

Query: 34  VTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTK 68
           VTVESLCSKQVEKE EVRGPPVSKAFDDQGNPTK
Sbjct: 333 VTVESLCSKQVEKEFEVRGPPVSKAFDDQGNPTK 366

BLAST of Spg034557 vs. TAIR 10
Match: AT3G48110.1 (glycine-tRNA ligases )

HSP 1 Score: 56.2 bits (134), Expect = 2.8e-08
Identity = 30/57 (52.63%), Postives = 37/57 (64.91%), Query Frame = 0

Query: 34  VTVESLCSKQVEKEVEVRGPPVSKAFDDQGNPTKNKLSIGLCYSSYQCKLKRRFKNK 91
           V V+++ SKQ+E+EVEVRGPP SKAFDD+GNPTK        Y     KL R+   K
Sbjct: 436 VLVDAMSSKQLEEEVEVRGPPASKAFDDEGNPTKAAEGFSRRYGVPLEKLYRKVSGK 492

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022973889.11.2e-0897.06glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucurbita maxima... [more]
XP_023520987.11.2e-0897.06glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita pepo s... [more]
XP_022973888.11.2e-0897.06glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita maxima... [more]
XP_023520988.11.2e-0897.06glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucurbita pepo s... [more]
XP_038874261.11.5e-0866.67glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Benincasa hispid... [more]
Match NameE-valueIdentityDescription
Q8L7853.9e-0752.63Glycine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1IFY65.6e-0997.06Glycine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111472479 PE=3 SV=1[more]
A0A6J1IEH35.6e-0997.06Glycine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111472479 PE=3 SV=1[more]
A0A6J1EYL51.6e-0894.12Glycine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111439724 PE=3 SV=1[more]
A0A6J1F3I31.6e-0894.12Glycine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111439724 PE=3 SV=1[more]
A0A1S4E2Q41.6e-0897.06Glycine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103498820 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48110.12.8e-0852.63glycine-tRNA ligases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 90..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg034557.1Spg034557.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0006426 glycyl-tRNA aminoacylation
biological_process GO:0045995 regulation of embryonic development
cellular_component GO:0009507 chloroplast
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0004820 glycine-tRNA ligase activity