Spg034478 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg034478 vs. NCBI nr
Match: XP_038875382.1 (uncharacterized protein At4g00950-like [Benincasa hispida])

HSP 1 Score: 275.8 bits (704), Expect = 2.9e-70
Identity = 143/202 (70.79%), Postives = 164/202 (81.19%), Query Frame = 0

Query: 4   KTEQNSSVARLPLFSIPH-QALDSPERSGMLTPPIYAVASVPFRWEEEPGKPRFST---I 63
           +TEQN SV+ LPL SIPH   +DSPERSG LTPPIYA  S+PFRWEEEPGKPRFS+    
Sbjct: 38  QTEQNPSVSILPLLSIPHLPIMDSPERSGTLTPPIYAAVSIPFRWEEEPGKPRFSSATVA 97

Query: 64  AAAAAASPSLELPPRLLLMERVPRSECSSSFRMMGGECY--DERSGLFMRKRGWFGFGFG 123
           +A+AAASPSLELPPRLLL+++VPRSEC SSFRMMG   +  DE+S LFM+K GW    FG
Sbjct: 98  SASAAASPSLELPPRLLLIDKVPRSECCSSFRMMGSSSFSPDEKSSLFMKKGGW----FG 157

Query: 124 SWRRRGFGLRSKRDDVGIGGLVFPSLELEESFDSKVETSRFRRNGSFSSL-TTQIRPHFW 183
           SWR+RG  LR KR+ +GIGGLVFPS+ELEES  SKVE SR RRNGSFSSL +T IRPHFW
Sbjct: 158 SWRKRGLNLRGKREQIGIGGLVFPSMELEESLSSKVEASRLRRNGSFSSLMSTHIRPHFW 217

Query: 184 ESVCESLKHVVP-WKSKRQKRD 198
           ESVCE LKH+VP W+SKR KR+
Sbjct: 218 ESVCEGLKHMVPSWRSKRLKRE 235

BLAST of Spg034478 vs. NCBI nr
Match: XP_008459763.1 (PREDICTED: uncharacterized protein At4g00950 [Cucumis melo])

HSP 1 Score: 261.5 bits (667), Expect = 5.7e-66
Identity = 137/197 (69.54%), Postives = 159/197 (80.71%), Query Frame = 0

Query: 6   EQNSSVARLPLFSIPHQAL-DSPERSGMLTPPIYAVASVPFRWEEEPGKPRFST-IAAAA 65
           +Q+SS   LPLFSIPH +L DSP+ SGMLTPPIY+  SVPFRWEEEPGKPRFST ++ +A
Sbjct: 12  DQDSSFENLPLFSIPHLSLMDSPQHSGMLTPPIYSAVSVPFRWEEEPGKPRFSTAVSISA 71

Query: 66  AASPSLELPPRLLLMERVPRSECSSSFRMMGGECYDERSGLFMRKRGWFGFGFGSWRRRG 125
           + SPSLELPPRLL    VPRSECSSSFR      +DE+S LFM+KRGW    FGSWR+RG
Sbjct: 72  STSPSLELPPRLL----VPRSECSSSFR------FDEKSSLFMKKRGW----FGSWRKRG 131

Query: 126 FGLRSKRDDVGIGGLVFPSLELEESFDSKVETSRFRRNGSFSSL--TTQIRPHFWESVCE 185
             LR KR+ +GIGGLVFPSLELEES  SKVE SRFRRNGSFSSL  +TQI+PHFWESVCE
Sbjct: 132 LNLRGKREQIGIGGLVFPSLELEESLGSKVEASRFRRNGSFSSLMSSTQIKPHFWESVCE 191

Query: 186 SLKHVVP-WKSKRQKRD 198
            LKH+VP W+S++ KR+
Sbjct: 192 GLKHIVPSWRSRKLKRE 194

BLAST of Spg034478 vs. NCBI nr
Match: XP_004140632.2 (uncharacterized protein At4g00950 [Cucumis sativus] >KGN46492.1 hypothetical protein Csa_005492 [Cucumis sativus])

HSP 1 Score: 259.6 bits (662), Expect = 2.2e-65
Identity = 140/204 (68.63%), Postives = 157/204 (76.96%), Query Frame = 0

Query: 1   MESKTEQ---NSSVARLPLFSIPH-QALDSPERSGMLTPPIYAVASVPFRWEEEPGKPRF 60
           ME K +Q   NSS   LPLFSIPH   +DSP+ SGMLTPPIY   SVPF WEEEPGKPRF
Sbjct: 4   MECKPQQRDHNSSFPNLPLFSIPHLSPMDSPQHSGMLTPPIYTAVSVPFWWEEEPGKPRF 63

Query: 61  STIAAAAAASPSLELPPRLLLMERVPRSECSSSFRMMGGECYDERSGLFMRKRGWFGFGF 120
           ST A + +ASPSLELPPRLL    VPRSECSSSFR      +DE+S LFM+KRGW    F
Sbjct: 64  ST-AVSVSASPSLELPPRLL----VPRSECSSSFR------FDEKSSLFMKKRGW----F 123

Query: 121 GSWRRRGFGLRSKRDDVGIGGLVFPSLELEESFDSKVETSRFRRNGSFSSL--TTQIRPH 180
           GSWR+RG  LR KR+ +G GGLVFPSLELEES  SKVE SRFRRNGSFSSL  +TQI+PH
Sbjct: 124 GSWRKRGLNLRGKREQIGTGGLVFPSLELEESLSSKVEASRFRRNGSFSSLMSSTQIKPH 183

Query: 181 FWESVCESLKHVVP-WKSKRQKRD 198
           FWESVCE LKH+VP W+S+R KR+
Sbjct: 184 FWESVCEGLKHIVPSWRSRRMKRE 192

BLAST of Spg034478 vs. NCBI nr
Match: XP_023005080.1 (uncharacterized protein LOC111498174 [Cucurbita maxima] >XP_023005081.1 uncharacterized protein LOC111498174 [Cucurbita maxima])

HSP 1 Score: 228.4 bits (581), Expect = 5.4e-56
Identity = 132/198 (66.67%), Postives = 144/198 (72.73%), Query Frame = 0

Query: 4   KTEQNSSVARLPLFSIPHQALDSPERSGMLTPPIYAVASVPFRWEEEPGKPRFSTIAAA- 63
           +T+ NSS+ARLPLFSIPHQA+DSPERSG LTPPIYA  SVPFRWEEEPGKPRFS +A   
Sbjct: 28  ETKPNSSLARLPLFSIPHQAMDSPERSGTLTPPIYAAVSVPFRWEEEPGKPRFSNVATTT 87

Query: 64  -AAASPSLELPPRLLLMERVPRSECSSSFRMMGGECYDERSGLFMRKRGWFGFGFGSWRR 123
               SP LELPPRLLLME+V RSE  SSFRMMG    DE S LFM++RGW    FGSWR 
Sbjct: 88  NTVTSPCLELPPRLLLMEKVARSE-GSSFRMMGS---DEMSSLFMKRRGW----FGSWR- 147

Query: 124 RGFGLRSKRDDVGIGGLVFPSLELEESFDSKVETSRFRRNGSFSSL-TTQIRPHFWESVC 183
                RSKRD+      VFPSLE E      VE SR RRNGSFSSL  TQIRP+FW SVC
Sbjct: 148 -----RSKRDE------VFPSLEWE------VEASRLRRNGSFSSLIRTQIRPNFWVSVC 199

Query: 184 ESLKHVVP-WKSKRQKRD 198
           E LKHVVP W+ KR K+D
Sbjct: 208 EGLKHVVPSWRGKRLKKD 199

BLAST of Spg034478 vs. NCBI nr
Match: XP_022960155.1 (uncharacterized protein At4g00950-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 226.1 bits (575), Expect = 2.7e-55
Identity = 131/202 (64.85%), Postives = 143/202 (70.79%), Query Frame = 0

Query: 4   KTEQNSSVARLPLFSIPHQALDSPERSGMLTPPIYAVASVPFRWEEEPGKPRFSTIAAA- 63
           +T+ NSS+ RLPLFSIPH+A+DSPERSG LTPPIYA  SVPFRWEEEPGKPRFS +A   
Sbjct: 7   ETKPNSSLTRLPLFSIPHRAMDSPERSGTLTPPIYAAVSVPFRWEEEPGKPRFSNVATTT 66

Query: 64  -----AAASPSLELPPRLLLMERVPRSECSSSFRMMGGECYDERSGLFMRKRGWFGFGFG 123
                   SP LELPPRLLLME+V RSE  SSFRMMG    DE S LFM++RGW    FG
Sbjct: 67  NTDTDTVTSPCLELPPRLLLMEKVGRSE-GSSFRMMGS---DEMSSLFMKRRGW----FG 126

Query: 124 SWRRRGFGLRSKRDDVGIGGLVFPSLELEESFDSKVETSRFRRNGSFSSL-TTQIRPHFW 183
           SWR      RSKRD+      VFPSLE E      VE SR RRNGSFSSL  TQIRPHFW
Sbjct: 127 SWR------RSKRDE------VFPSLEWE------VEASRLRRNGSFSSLIRTQIRPHFW 182

Query: 184 ESVCESLKHVVP-WKSKRQKRD 198
            SVCE LKHVVP W+ KR K+D
Sbjct: 187 VSVCEGLKHVVPSWRGKRLKKD 182

BLAST of Spg034478 vs. ExPASy Swiss-Prot
Match: Q9M160 (Uncharacterized protein At4g00950 OS=Arabidopsis thaliana OX=3702 GN=At4g00950 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.3e-12
Identity = 84/292 (28.77%), Postives = 118/292 (40.41%), Query Frame = 0

Query: 2   ESKTEQNSSVARLPLFSIPHQALDSPERSGMLTPPIYA--VASVPFRWEEEPGKPRFSTI 61
           E++ E N +V +LP+             S  ++ PI++   ASVPF WEEEPGKP+  + 
Sbjct: 6   ETEQEGNLTVMKLPVLPTKPNT-----HSHSMSSPIHSSISASVPFSWEEEPGKPKQHST 65

Query: 62  AAAAAAS---------------PSLELPPRLLLMER----------------------VP 121
           ++++++S                SLELPPRL L+E+                        
Sbjct: 66  SSSSSSSSSPLTSYSSSPFETHKSLELPPRLHLLEKDGGSVTKLHSPITVFDGPYSMTTS 125

Query: 122 RSECSSSFRMM---GGECYDE-RSGL-----------------------FMRKRGWFGFG 181
           +   S SFRMM     +CY   RS +                        ++KRG  GF 
Sbjct: 126 KRMDSPSFRMMVKGSADCYGSFRSDIDGDLEDLEVGSKQQENLSSGSLAVVKKRGRLGF- 185

Query: 182 FGSWRRRGFGLRSKRDDVGIGGLVFPSL-----------ELEESFDSK------------ 194
           FG  RRR       + + G G  VFPS            E EE  D +            
Sbjct: 186 FGFRRRRAL---KGKTEFGRGSYVFPSSVDRESEYSRKEEEEEKEDKRFGYDDTDGISCS 245

BLAST of Spg034478 vs. ExPASy TrEMBL
Match: A0A1S3CBE3 (uncharacterized protein At4g00950 OS=Cucumis melo OX=3656 GN=LOC103498800 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 2.8e-66
Identity = 137/197 (69.54%), Postives = 159/197 (80.71%), Query Frame = 0

Query: 6   EQNSSVARLPLFSIPHQAL-DSPERSGMLTPPIYAVASVPFRWEEEPGKPRFST-IAAAA 65
           +Q+SS   LPLFSIPH +L DSP+ SGMLTPPIY+  SVPFRWEEEPGKPRFST ++ +A
Sbjct: 12  DQDSSFENLPLFSIPHLSLMDSPQHSGMLTPPIYSAVSVPFRWEEEPGKPRFSTAVSISA 71

Query: 66  AASPSLELPPRLLLMERVPRSECSSSFRMMGGECYDERSGLFMRKRGWFGFGFGSWRRRG 125
           + SPSLELPPRLL    VPRSECSSSFR      +DE+S LFM+KRGW    FGSWR+RG
Sbjct: 72  STSPSLELPPRLL----VPRSECSSSFR------FDEKSSLFMKKRGW----FGSWRKRG 131

Query: 126 FGLRSKRDDVGIGGLVFPSLELEESFDSKVETSRFRRNGSFSSL--TTQIRPHFWESVCE 185
             LR KR+ +GIGGLVFPSLELEES  SKVE SRFRRNGSFSSL  +TQI+PHFWESVCE
Sbjct: 132 LNLRGKREQIGIGGLVFPSLELEESLGSKVEASRFRRNGSFSSLMSSTQIKPHFWESVCE 191

Query: 186 SLKHVVP-WKSKRQKRD 198
            LKH+VP W+S++ KR+
Sbjct: 192 GLKHIVPSWRSRKLKRE 194

BLAST of Spg034478 vs. ExPASy TrEMBL
Match: A0A0A0KDR5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G103510 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.1e-65
Identity = 140/204 (68.63%), Postives = 157/204 (76.96%), Query Frame = 0

Query: 1   MESKTEQ---NSSVARLPLFSIPH-QALDSPERSGMLTPPIYAVASVPFRWEEEPGKPRF 60
           ME K +Q   NSS   LPLFSIPH   +DSP+ SGMLTPPIY   SVPF WEEEPGKPRF
Sbjct: 4   MECKPQQRDHNSSFPNLPLFSIPHLSPMDSPQHSGMLTPPIYTAVSVPFWWEEEPGKPRF 63

Query: 61  STIAAAAAASPSLELPPRLLLMERVPRSECSSSFRMMGGECYDERSGLFMRKRGWFGFGF 120
           ST A + +ASPSLELPPRLL    VPRSECSSSFR      +DE+S LFM+KRGW    F
Sbjct: 64  ST-AVSVSASPSLELPPRLL----VPRSECSSSFR------FDEKSSLFMKKRGW----F 123

Query: 121 GSWRRRGFGLRSKRDDVGIGGLVFPSLELEESFDSKVETSRFRRNGSFSSL--TTQIRPH 180
           GSWR+RG  LR KR+ +G GGLVFPSLELEES  SKVE SRFRRNGSFSSL  +TQI+PH
Sbjct: 124 GSWRKRGLNLRGKREQIGTGGLVFPSLELEESLSSKVEASRFRRNGSFSSLMSSTQIKPH 183

Query: 181 FWESVCESLKHVVP-WKSKRQKRD 198
           FWESVCE LKH+VP W+S+R KR+
Sbjct: 184 FWESVCEGLKHIVPSWRSRRMKRE 192

BLAST of Spg034478 vs. ExPASy TrEMBL
Match: A0A6J1KTY4 (uncharacterized protein LOC111498174 OS=Cucurbita maxima OX=3661 GN=LOC111498174 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 2.6e-56
Identity = 132/198 (66.67%), Postives = 144/198 (72.73%), Query Frame = 0

Query: 4   KTEQNSSVARLPLFSIPHQALDSPERSGMLTPPIYAVASVPFRWEEEPGKPRFSTIAAA- 63
           +T+ NSS+ARLPLFSIPHQA+DSPERSG LTPPIYA  SVPFRWEEEPGKPRFS +A   
Sbjct: 28  ETKPNSSLARLPLFSIPHQAMDSPERSGTLTPPIYAAVSVPFRWEEEPGKPRFSNVATTT 87

Query: 64  -AAASPSLELPPRLLLMERVPRSECSSSFRMMGGECYDERSGLFMRKRGWFGFGFGSWRR 123
               SP LELPPRLLLME+V RSE  SSFRMMG    DE S LFM++RGW    FGSWR 
Sbjct: 88  NTVTSPCLELPPRLLLMEKVARSE-GSSFRMMGS---DEMSSLFMKRRGW----FGSWR- 147

Query: 124 RGFGLRSKRDDVGIGGLVFPSLELEESFDSKVETSRFRRNGSFSSL-TTQIRPHFWESVC 183
                RSKRD+      VFPSLE E      VE SR RRNGSFSSL  TQIRP+FW SVC
Sbjct: 148 -----RSKRDE------VFPSLEWE------VEASRLRRNGSFSSLIRTQIRPNFWVSVC 199

Query: 184 ESLKHVVP-WKSKRQKRD 198
           E LKHVVP W+ KR K+D
Sbjct: 208 EGLKHVVPSWRGKRLKKD 199

BLAST of Spg034478 vs. ExPASy TrEMBL
Match: A0A6J1H8B1 (uncharacterized protein At4g00950-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460982 PE=4 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 1.3e-55
Identity = 131/202 (64.85%), Postives = 143/202 (70.79%), Query Frame = 0

Query: 4   KTEQNSSVARLPLFSIPHQALDSPERSGMLTPPIYAVASVPFRWEEEPGKPRFSTIAAA- 63
           +T+ NSS+ RLPLFSIPH+A+DSPERSG LTPPIYA  SVPFRWEEEPGKPRFS +A   
Sbjct: 7   ETKPNSSLTRLPLFSIPHRAMDSPERSGTLTPPIYAAVSVPFRWEEEPGKPRFSNVATTT 66

Query: 64  -----AAASPSLELPPRLLLMERVPRSECSSSFRMMGGECYDERSGLFMRKRGWFGFGFG 123
                   SP LELPPRLLLME+V RSE  SSFRMMG    DE S LFM++RGW    FG
Sbjct: 67  NTDTDTVTSPCLELPPRLLLMEKVGRSE-GSSFRMMGS---DEMSSLFMKRRGW----FG 126

Query: 124 SWRRRGFGLRSKRDDVGIGGLVFPSLELEESFDSKVETSRFRRNGSFSSL-TTQIRPHFW 183
           SWR      RSKRD+      VFPSLE E      VE SR RRNGSFSSL  TQIRPHFW
Sbjct: 127 SWR------RSKRDE------VFPSLEWE------VEASRLRRNGSFSSLIRTQIRPHFW 182

Query: 184 ESVCESLKHVVP-WKSKRQKRD 198
            SVCE LKHVVP W+ KR K+D
Sbjct: 187 VSVCEGLKHVVPSWRGKRLKKD 182

BLAST of Spg034478 vs. ExPASy TrEMBL
Match: A0A6J1H6U8 (uncharacterized protein At4g00950-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111460982 PE=4 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 4.9e-47
Identity = 116/180 (64.44%), Postives = 126/180 (70.00%), Query Frame = 0

Query: 4   KTEQNSSVARLPLFSIPHQALDSPERSGMLTPPIYAVASVPFRWEEEPGKPRFSTIAAA- 63
           +T+ NSS+ RLPLFSIPH+A+DSPERSG LTPPIYA  SVPFRWEEEPGKPRFS +A   
Sbjct: 7   ETKPNSSLTRLPLFSIPHRAMDSPERSGTLTPPIYAAVSVPFRWEEEPGKPRFSNVATTT 66

Query: 64  -----AAASPSLELPPRLLLMERVPRSECSSSFRMMGGECYDERSGLFMRKRGWFGFGFG 123
                   SP LELPPRLLLME+V RSE  SSFRMMG    DE S LFM++RGW    FG
Sbjct: 67  NTDTDTVTSPCLELPPRLLLMEKVGRSE-GSSFRMMGS---DEMSSLFMKRRGW----FG 126

Query: 124 SWRRRGFGLRSKRDDVGIGGLVFPSLELEESFDSKVETSRFRRNGSFSSL-TTQIRPHFW 177
           SWR      RSKRD+      VFPSLE E      VE SR RRNGSFSSL  TQIRPHFW
Sbjct: 127 SWR------RSKRDE------VFPSLEWE------VEASRLRRNGSFSSLIRTQIRPHFW 160

BLAST of Spg034478 vs. TAIR 10
Match: AT4G00950.1 (Protein of unknown function (DUF688) )

HSP 1 Score: 74.7 bits (182), Expect = 9.3e-14
Identity = 84/292 (28.77%), Postives = 118/292 (40.41%), Query Frame = 0

Query: 2   ESKTEQNSSVARLPLFSIPHQALDSPERSGMLTPPIYA--VASVPFRWEEEPGKPRFSTI 61
           E++ E N +V +LP+             S  ++ PI++   ASVPF WEEEPGKP+  + 
Sbjct: 6   ETEQEGNLTVMKLPVLPTKPNT-----HSHSMSSPIHSSISASVPFSWEEEPGKPKQHST 65

Query: 62  AAAAAAS---------------PSLELPPRLLLMER----------------------VP 121
           ++++++S                SLELPPRL L+E+                        
Sbjct: 66  SSSSSSSSSPLTSYSSSPFETHKSLELPPRLHLLEKDGGSVTKLHSPITVFDGPYSMTTS 125

Query: 122 RSECSSSFRMM---GGECYDE-RSGL-----------------------FMRKRGWFGFG 181
           +   S SFRMM     +CY   RS +                        ++KRG  GF 
Sbjct: 126 KRMDSPSFRMMVKGSADCYGSFRSDIDGDLEDLEVGSKQQENLSSGSLAVVKKRGRLGF- 185

Query: 182 FGSWRRRGFGLRSKRDDVGIGGLVFPSL-----------ELEESFDSK------------ 194
           FG  RRR       + + G G  VFPS            E EE  D +            
Sbjct: 186 FGFRRRRAL---KGKTEFGRGSYVFPSSVDRESEYSRKEEEEEKEDKRFGYDDTDGISCS 245

BLAST of Spg034478 vs. TAIR 10
Match: AT5G53030.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF688 (InterPro:IPR007789); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G27810.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 73.2 bits (178), Expect = 2.7e-13
Identity = 71/229 (31.00%), Postives = 94/229 (41.05%), Query Frame = 0

Query: 8   NSSVARLPLFSIPHQALDSPERSGMLTPPIYAVASVPFRWEEEPGKPRFSTIAA---AAA 67
           +S+  +LPLFS P   +      G+ TPP+    SVPF WEE PGKPR     A      
Sbjct: 13  SSTRKQLPLFSYPMNNIAYETTPGLATPPVNIAGSVPFLWEEAPGKPRRVKKPARLNQKG 72

Query: 68  ASPSLELPPRLLL-------MERVPRSECSSSFRMMGGECYDERSGLFMRK-RG------ 127
              SLELPPRL+L        E  P +     + +        RS   +RK RG      
Sbjct: 73  VVRSLELPPRLVLPGESTTVNEPSPTTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAP 132

Query: 128 ------------WFGFG---------FGSWRRRGFGLRSKRDDVGIGGLVFPSLELEESF 187
                       W  FG         F   R R  G   +RD  G GG+         + 
Sbjct: 133 EKEERLVGGSSRWGSFGNCKEVSEGIFDFSRFRDDGYDCRRDWAGGGGVG------NFAG 192

Query: 188 DSKVETSRFRRNGSFSSLTTQIRPHFW----ESVCESLKHVVPWKSKRQ 195
           D+KV+  R  + GSF +L+   +  FW      V E  K V+PWK K++
Sbjct: 193 DAKVKLYRIIKKGSFFNLSHTTKSDFWLKMQARVYEGFKQVIPWKRKQE 235

BLAST of Spg034478 vs. TAIR 10
Match: AT2G46535.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF688 (InterPro:IPR007789); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF688) (TAIR:AT3G61840.1); Has 48 Blast hits to 48 proteins in 8 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 48; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 65.9 bits (159), Expect = 4.3e-11
Identity = 57/174 (32.76%), Postives = 81/174 (46.55%), Query Frame = 0

Query: 26  SPERSGMLTPPIYAVASVPFRWEEEPGKPRFSTIAAAAAASPSLELPPRLLLMERVPRSE 85
           SP    +   PI+ +ASVPF WE++PGKP+        +    L+LPPRLLL        
Sbjct: 28  SPASPRVFASPIHTLASVPFCWEDQPGKPKHP--LRPLSYPKCLDLPPRLLL-------- 87

Query: 86  CSSSFRMMGGECYDERSGLFMRKRGWFGFGFGSWRRRGFGLRSKRDDVGIGGLVFPSLEL 145
               F  M          L  RK G   F     RR+G G     D V  G  VF S E 
Sbjct: 88  -PGEFTQM---------PLPERKHGLLRF----LRRKGRG-----DVVVRGNYVFLS-EN 147

Query: 146 EESFD----SKVETSRFRRNGSFSSLTTQIRPHFWESVCESLKHVVPWKSKRQK 196
           + + D    + ++  +F R+GS+    +    HFW S+C+ LK  +PWK+K+ +
Sbjct: 148 QRAGDNINENNMKIMKFNRSGSYHGGGSVKGSHFWGSLCKGLKLAMPWKNKKMR 171

BLAST of Spg034478 vs. TAIR 10
Match: AT4G27810.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF688 (InterPro:IPR007789); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G53030.1); Has 73 Blast hits to 66 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 73; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 64.3 bits (155), Expect = 1.3e-10
Identity = 63/208 (30.29%), Postives = 91/208 (43.75%), Query Frame = 0

Query: 6   EQNSSVARLPLFSIP-HQALDSPERSGMLTPPIYAVASVPFRWEEEPGKPRFSTIAAAAA 65
           E+  +  +LPLFSIP ++A D+P   G+ TPP+    SVPF WEE PGKPR S      A
Sbjct: 9   ERFCATPKLPLFSIPFNRACDTP---GLATPPVNIAGSVPFLWEEAPGKPRVSDENKPLA 68

Query: 66  ASPS------------LELPPRLLLMERVPRSECSSSFRMMGGECYDERSGLFMRKR--- 125
           +  +            LELPPRL      P  +  S   ++ G     R  L + +R   
Sbjct: 69  SKQNEREGGGGGVVRCLELPPRLFF----PADDEPSPTTVLDGPYDVPRRSLSVIRRSER 128

Query: 126 ---GWFGFGFGSWRRRGFGLRSKRDDVGIGGLVFPSLELEESFDSKVETSRFRRNGSFSS 185
              G F F   +         + R   G GG             + V+ SR RR GS  +
Sbjct: 129 ASEGRFEFSRST---------NSRCCDGGGG-------------TTVKISRVRRKGSLLN 186

Query: 186 LTTQIRPHFWESVCESLKHVVPWKSKRQ 195
           L +  +  F   V +  K V+PW+ +++
Sbjct: 189 L-SHSKSQFLARVYQGFKQVIPWRRRQE 186

BLAST of Spg034478 vs. TAIR 10
Match: AT5G53030.2 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF688 (InterPro:IPR007789); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G27810.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 61.6 bits (148), Expect = 8.1e-10
Identity = 64/209 (30.62%), Postives = 84/209 (40.19%), Query Frame = 0

Query: 8   NSSVARLPLFSIPHQALDSPERSGMLTPPIYAVASVPFRWEEEPGKPRFSTIAA---AAA 67
           +S+  +LPLFS P   +      G+ TPP+    SVPF WEE PGKPR     A      
Sbjct: 13  SSTRKQLPLFSYPMNNIAYETTPGLATPPVNIAGSVPFLWEEAPGKPRRVKKPARLNQKG 72

Query: 68  ASPSLELPPRLLL-------MERVPRSECSSSFRMMGGECYDERSGLFMRK-RG------ 127
              SLELPPRL+L        E  P +     + +        RS   +RK RG      
Sbjct: 73  VVRSLELPPRLVLPGESTTVNEPSPTTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAP 132

Query: 128 ------------WFGFG---------FGSWRRRGFGLRSKRDDVGIGGLVFPSLELEESF 179
                       W  FG         F   R R  G   +RD  G GG+         + 
Sbjct: 133 EKEERLVGGSSRWGSFGNCKEVSEGIFDFSRFRDDGYDCRRDWAGGGGVG------NFAG 192