Spg034201 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg034201
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	FSH1 domain-containing protein
Location	scaffold13: 33762918 .. 33766757 (-)
RNA-Seq Expression	Spg034201
Synteny	Spg034201

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACCACATACCTCTCTATTATTCATTTATGTATTGTTTTGCCAATTTTTTTTTTCAGAACAAACAAAAACGCGGCGTATTTCAAACCATTACAAAATATACATGTTTACTTCTAGTTCTAGAAACAATTAGCCCATTAAAAAAAATGCCCATATGAGAGTTTAAAAAACAAAACAGAGTCAAAGCGAGCTTAGCTCAGCGGTAATTGGCATGCACCATCGACCATGAGATCAAATGTTCAATCTCCCATCCCAAATATTGATGTACTAAAAAAAATAAATGAAAATAAAAAACAAAACAAAAAATAATAAAATAAGAAAAAACCCAGATGGTATAATTGTAATTTACTTCCTCCCCTAAGCCAACCCGTAGCTTCCCGGTTTCCAGAATTCATTAATTTATCAATACAATGCACTTAATCCCGAAAAGAACCCTCTCAAGTGCACGTCCCTTTCCCCAAAATAAATACACAAATAAACAAATAAATACCCGCGTCAGCACGTTCGTTTTTGACGGTACCCATTTTTAAAACGAGTCAATTTTTTAAGCTTCCCACTGAGGGGGAAGGGCCCAGAAAATGCCTTTAAAATGAGTTCATTCTCCCCTTCCCTCTGCTCTCATCATCAGCTCCTCACACCAATTTCCATACCGAGATCTCCAGAATCCGACTCACAGAGAATTTCAACCATGGGAAGCGACGGAAGAATCCCGAGAAAACCTAGATTTCTTTGCCTCCATGGCTTCCGAACCAGTGGAGAAATCCTCAAGAAGCAGGTTGGGAAATGGCCGGCTTCTGTCCTCGATCAATTCGATCTCGATTTCCTCGACGCTCCATTTCCTGCAGAAGGAAAATCCGATGTTGAAGGAATTTTCGATCCTCCCTATTTCGAGTGGTTTCAATTCAGTGCGGTACGATTGCTTGACCTAATTTCAATTTCTTTTCAAATTCGCAAACTCCTGTTTAATTTATTTATTTTCTCCCTTCTTCCATTACAGGAATTTACAGAATACAGAAATTTCGACGAGTGTCTCTCATTCATTGAAGATTACATGCTTAAGCACGGACCATTTGATGGATTCCTCGGTTTCTCACAGGTAATTATCCTGAATTTCCTCTGTTCTTTTCTTAATTGGCGAAATAAAGCTTAATCAAGTATCAATTTGGAGTTGAATTTCACAATTTCAATCTCTTCGCGAACATTTTCAGGGGGCGATATTATCGGCCGCGCTGCCGGGACTTCAAGCAAAGGTAACCTAATCGGAGCCTGCTGCTCTTACAAATCATTTTGAATTCTACAAAATAAATGTTAATTCGAAATTGAGATTTTGACGCGGCGGTCTTTCGATTAATCGGTTGCAGGGTGTTGCTTTGACGAAAGTACCGAAGATCAAATTCGTGATCATCGTCAGCGGTGCGAAATTCAGATCGCAATCTGTGGCTGAGAAAGCTTACTCGACAGCCATTGGATGCCCGTCCCTCCATTTTCTAGGTACCTTCACATGTTTTTGTTTCTTTTTGGAAAGGCATACACACTGCGTTGTTTTCAAATATTCTGATTCTTTGCTTTTTTTTTTTTTTTTTTTTGGCTAAATTATAAAAAAAATATCACTGAACTATGAGGTTTATTTAAAAAATGTTCCTGAACTTTGAAAAGTTTAAAAAATACTCCTGAACCTTGAAAATGTTTCAAAAATACCCTTGCCATTTAGTTTTGGATGAAAACCGTTAAAGTTTCAGTTACAAAATAAGCTTGAACTTTCAAAAAAATTTAAAAAATACCCTTATTGAAAAATGTTTTCTTTTTTCCTCTTCTCACCGTAAATTCTAACGGTTTTCATCCAAAAATTAACTGCAAGGGTATTTTTGAAATATTTTTTAAAGTTCAGGGGTATTTTTGAAACATTTTTCAAAGTTCAGGGGTATTTTTGAAACTTTTCAAAGTTTAGGAGCATTTTTGAAACAAACCATTTTTTATAATTTAGCCTTTTTTTTCTTTCTTTTTGAAACTTGTTTCTTTGCACTGTTTTTTTTTTTTTTTTAAATATGATTCTTTGCCCTGTTATTTGTAAAAGAACCGACCACTGAATGCATAATTTTAGGTCAGGATTAAGTATATGTCTGTATGATTTTTTAAGTGTTTAAAAATATATTTAAAAATATTTAAAATGTTATTTCAAACGGATAAAATAAGCGTGTGAAAATTTCTATTAAGATATACTCATATACATATAAAATAAAAAAATATATATCTATAAAAGCATTTAATTTCAATTAATGATGAAGTTCTTTTCTTCTGTAAAAATATTATAGATCTTATTATTAGTATTTATATAGAACTAATTGGTGATATTATTAATATTTTATGAGTTCTAACGCGGGATATTAAATATATTTTAAATGGCTACCAAAGCAAGCTCAAATAACATAAAACTTATTCTTGACCAAGAGGTTAGAGGAACAAATTACCTCCTCCAAATGTTGTAAAGAAAAAAAAGATATGTTTTGAATCTTAATCCTGTTGAACTCTTTAGATGTAGCTATGTACGGATATTTTAATCTTTGATTTTAAACGATTTCTTCAAATTTTAATAATAATCTACAATTTTTTTGCAATATTTAGTCTCGAAATAAAACAAAACTCATATCTTAATTAATCGAGCTATATTCGAGTTTATACCTATTATATATGGATAATACTCTCCTACATTAATAGACCAGAATAATAGATAGATAGGTCCAGAATTGTTTATTGTGAAGTAGGTTCTTTATTGTTTAATTTAAGATGCCATCAATGGAAGTGTTGACTAAAGTTATATGAAATGTTTAGTGAATATAATTTTAAAATAAATTATTATTAAAATTTGAAATAATTAATCAACTTTGTGTTATTTAACGGTGGCAGGGGAGGAAGACTTCTTGAAGCCTCACGGATTGGAGCTGCTGGAATCATACGTTGAACCATTAGTAATCAATCATCCTAAAGGCCACACAGTACCTAGACTTGGTAATATTTAATGGAAAATTGCAGTGGGTGGCAAATTGGACCAAATTAATAGCAAATATAGCACCATGTAAAAAAAATTACAAATATAGCAAAATCTATCGGAGTCTATCTGATAGACTTCGATTATCAGAGTTTATCAGCGATAGACTCTGATTAATCAGAATCTATCAGTCTACTGATAGACTCCGATTATTCAGAGTCTATCAGTGTCTATCGTTGATAAACTCTGGTTAATCAAAATCTATCAGTGTCTATCACTGATAGACTTTGATTAATCGAAGTCTATCAGCGATAGACACTGATATACTTTGAATAATCAGAGCCTATCAGTGATAGACACGGATAGAATCCGACAACAGCTTTTTGGTATTGTACAATTAATTTTGCTATATTTGCAATTAGTTTGAAACTGTGCTATATTTGCTATTAATTTGGTCCAATTTGCCATCTATGCAACTGCCCCATATTTAATTTCACTTTTTCATAAAAAAGAAAATACATATATATTTTTTTTAATTTCTGACGCTAATTTAGATTATGTGAACCTTATTTTTTCAGATGATAAAGGTTTGGAGGTTATGGAGAGTTTCATTGAGAGGATTTCAAAGACGATGGATGAGACTAAAGAATAAGCCTGAAATTATGTTGGATTATCAATTATGCAATTTTACTCCTTGAAGAACAAATAGAAAACAATGTTGGAGTTAATTGTAATTGTAATACAATCTTCATTTTCATGATGTAGTGACAATTTTAGTATTCTCTCTACAACCATCATTCTGTTAATTTCTCTAATAATTTGTTGGTACAAGCAATGCTAATTCTTTTAGCAATAG

mRNA sequence

ATGAGTTCATTCTCCCCTTCCCTCTGCTCTCATCATCAGCTCCTCACACCAATTTCCATACCGAGATCTCCAGAATCCGACTCACAGAGAATTTCAACCATGGGAAGCGACGGAAGAATCCCGAGAAAACCTAGATTTCTTTGCCTCCATGGCTTCCGAACCAGTGGAGAAATCCTCAAGAAGCAGGTTGGGAAATGGCCGGCTTCTGTCCTCGATCAATTCGATCTCGATTTCCTCGACGCTCCATTTCCTGCAGAAGGAAAATCCGATGTTGAAGGAATTTTCGATCCTCCCTATTTCGAGTGGTTTCAATTCAGTGCGGAATTTACAGAATACAGAAATTTCGACGAGTGTCTCTCATTCATTGAAGATTACATGCTTAAGCACGGACCATTTGATGGATTCCTCGGTTTCTCACAGGGGGCGATATTATCGGCCGCGCTGCCGGGACTTCAAGCAAAGGGTGTTGCTTTGACGAAAGTACCGAAGATCAAATTCGTGATCATCGTCAGCGGTGCGAAATTCAGATCGCAATCTGTGGCTGAGAAAGCTTACTCGACAGCCATTGGATGCCCGTCCCTCCATTTTCTAGGGGAGGAAGACTTCTTGAAGCCTCACGGATTGGAGCTGCTGGAATCATACGTTGAACCATTAGTAATCAATCATCCTAAAGGCCACACAGTACCTAGACTTGATGATAAAGGTTTGGAGGTTATGGAGAGTTTCATTGAGAGGATTTCAAAGACGATGGATGAGACTAAAGAATAA

Coding sequence (CDS)

ATGAGTTCATTCTCCCCTTCCCTCTGCTCTCATCATCAGCTCCTCACACCAATTTCCATACCGAGATCTCCAGAATCCGACTCACAGAGAATTTCAACCATGGGAAGCGACGGAAGAATCCCGAGAAAACCTAGATTTCTTTGCCTCCATGGCTTCCGAACCAGTGGAGAAATCCTCAAGAAGCAGGTTGGGAAATGGCCGGCTTCTGTCCTCGATCAATTCGATCTCGATTTCCTCGACGCTCCATTTCCTGCAGAAGGAAAATCCGATGTTGAAGGAATTTTCGATCCTCCCTATTTCGAGTGGTTTCAATTCAGTGCGGAATTTACAGAATACAGAAATTTCGACGAGTGTCTCTCATTCATTGAAGATTACATGCTTAAGCACGGACCATTTGATGGATTCCTCGGTTTCTCACAGGGGGCGATATTATCGGCCGCGCTGCCGGGACTTCAAGCAAAGGGTGTTGCTTTGACGAAAGTACCGAAGATCAAATTCGTGATCATCGTCAGCGGTGCGAAATTCAGATCGCAATCTGTGGCTGAGAAAGCTTACTCGACAGCCATTGGATGCCCGTCCCTCCATTTTCTAGGGGAGGAAGACTTCTTGAAGCCTCACGGATTGGAGCTGCTGGAATCATACGTTGAACCATTAGTAATCAATCATCCTAAAGGCCACACAGTACCTAGACTTGATGATAAAGGTTTGGAGGTTATGGAGAGTTTCATTGAGAGGATTTCAAAGACGATGGATGAGACTAAAGAATAA

Protein sequence

MSSFSPSLCSHHQLLTPISIPRSPESDSQRISTMGSDGRIPRKPRFLCLHGFRTSGEILKKQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGIFDPPYFEWFQFSAEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTKVPKIKFVIIVSGAKFRSQSVAEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYVEPLVINHPKGHTVPRLDDKGLEVMESFIERISKTMDETKE

Homology

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BLAST of Spg034201 vs. NCBI nr
Match: XP_023545459.1 (dihydrofolate reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 441.4 bits (1134), Expect = 5.2e-120
Identity = 217/255 (85.10%), Postives = 230/255 (90.20%), Query Frame = 0


Query: 1   MSSFSPSLCSHHQLLTPISIPRSPESDSQRISTMGSDGRIPRKPRFLCLHGFRTSGEILK 60
           MSS SP LCS      PIS  RSPE   QRI  MG+DG I RKPRFLCLHGFRTSG ILK
Sbjct: 1   MSSLSPLLCS------PISKYRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILK 60

Query: 61  KQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGIFDPPYFEWFQFSAEFTEYRNFDECLS 120
           KQVGKWP SVL+Q DLDFLDAPFPAEGKSD+EGIFDPPYFEWFQFS EFTEYRNFDECLS
Sbjct: 61  KQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLS 120

Query: 121 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTKVPKIKFVIIVSGAKFRSQSV 180
           FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKG+ALTKVPKIKFVIIVSGAKFRSQSV
Sbjct: 121 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSV 180

Query: 181 AEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYVEPLVINHPKGHTVPRLDDKGLEVME 240
           AEKAYS+AIGCPSLHFLG+EDFL PHG +LLESY+EP+VINHPKGHT+PRLDDKGLEVME
Sbjct: 181 AEKAYSSAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVME 240

Query: 241 SFIERISKTMDETKE 256
           SFIE+ISK +DE +E
Sbjct: 241 SFIEKISKMLDEAEE 249

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BLAST of Spg034201 vs. NCBI nr
Match: XP_022997250.1 (dihydrofolate reductase-like [Cucurbita maxima])

HSP 1 Score: 440.3 bits (1131), Expect = 1.2e-119
Identity = 216/255 (84.71%), Postives = 230/255 (90.20%), Query Frame = 0


Query: 1   MSSFSPSLCSHHQLLTPISIPRSPESDSQRISTMGSDGRIPRKPRFLCLHGFRTSGEILK 60
           MSS SP LCS      PIS  RSPE   QRI  MG+DG I RKPRFLCLHGFRTSG ILK
Sbjct: 1   MSSLSPLLCS------PISKTRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILK 60

Query: 61  KQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGIFDPPYFEWFQFSAEFTEYRNFDECLS 120
           KQVGKWP SVL+Q DLDFLDAPFPAEGKSD+EGIFDPPYFEWFQFS EFTEYRNFDECLS
Sbjct: 61  KQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLS 120

Query: 121 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTKVPKIKFVIIVSGAKFRSQSV 180
           FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKG+ALTKVPKIKFVIIVSGAKFRSQSV
Sbjct: 121 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGMALTKVPKIKFVIIVSGAKFRSQSV 180

Query: 181 AEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYVEPLVINHPKGHTVPRLDDKGLEVME 240
           AEKAYS+AIGCPSLHFLG+EDFL PHG +LLESY+EP+VINHPKGHT+PRLDD+GLEVME
Sbjct: 181 AEKAYSSAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDRGLEVME 240

Query: 241 SFIERISKTMDETKE 256
           SFIE+ISK +DE +E
Sbjct: 241 SFIEKISKMLDEAEE 249

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BLAST of Spg034201 vs. NCBI nr
Match: XP_022961839.1 (dihydrofolate reductase-like [Cucurbita moschata])

HSP 1 Score: 438.0 bits (1125), Expect = 5.8e-119
Identity = 214/255 (83.92%), Postives = 228/255 (89.41%), Query Frame = 0


Query: 1   MSSFSPSLCSHHQLLTPISIPRSPESDSQRISTMGSDGRIPRKPRFLCLHGFRTSGEILK 60
           MSS SP LCS      PIS  RSPE   QRI  MG+DG I RKPRFLCLHGFRTSG ILK
Sbjct: 1   MSSLSPLLCS------PISKSRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILK 60

Query: 61  KQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGIFDPPYFEWFQFSAEFTEYRNFDECLS 120
           KQVGKWP SVL+Q DLDFLDAPFPAEGKSD+EGIFDPPYFEWFQFS EFTEYRNFDECLS
Sbjct: 61  KQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLS 120

Query: 121 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTKVPKIKFVIIVSGAKFRSQSV 180
           FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKG+ALTKVPKIKFVIIVSGAKFRSQSV
Sbjct: 121 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSV 180

Query: 181 AEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYVEPLVINHPKGHTVPRLDDKGLEVME 240
           AEKAYS  IGCPSLHFLG+EDFL PHG +LLESY+EP++INHPKGHT+PRLDD+GLEVME
Sbjct: 181 AEKAYSPVIGCPSLHFLGKEDFLMPHGQKLLESYIEPVIINHPKGHTIPRLDDEGLEVME 240

Query: 241 SFIERISKTMDETKE 256
           SFIE+ISK +DE +E
Sbjct: 241 SFIEKISKMLDEAEE 249

[Close]

BLAST of Spg034201 vs. NCBI nr
Match: XP_038885360.1 (dihydrofolate reductase-like [Benincasa hispida])

HSP 1 Score: 424.5 bits (1090), Expect = 6.6e-115
Identity = 204/228 (89.47%), Postives = 214/228 (93.86%), Query Frame = 0


Query: 28  SQRISTMGSDGRIPRKPRFLCLHGFRTSGEILKKQVGKWPASVLDQFDLDFLDAPFPAEG 87
           + RI  MG+D +I RKPRFLCLHGFRTS  ILKKQVGKWPASVLDQFDL FLDAPFPAEG
Sbjct: 11  NSRIPIMGTDAKIHRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQFDLHFLDAPFPAEG 70

Query: 88  KSDVEGIFDPPYFEWFQFSAEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAA 147
           KSDVEGIFDPPYFEWFQFS EFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAA
Sbjct: 71  KSDVEGIFDPPYFEWFQFSQEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAA 130

Query: 148 LPGLQAKGVALTKVPKIKFVIIVSGAKFRSQSVAEKAYSTAIGCPSLHFLGEEDFLKPHG 207
           LPG QAKG+ALTKVPKIKFVIIVSGAKFRS+SVA+KAYST IGCPSLHFLGEEDFL PHG
Sbjct: 131 LPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAQKAYSTPIGCPSLHFLGEEDFLMPHG 190

Query: 208 LELLESYVEPLVINHPKGHTVPRLDDKGLEVMESFIERISKTMDETKE 256
            +LLESYVEP VINHPKGHTVPRLDDKGLEVMESFI+RISKT+DET+E
Sbjct: 191 QKLLESYVEPTVINHPKGHTVPRLDDKGLEVMESFIKRISKTLDETEE 238

[Close]

BLAST of Spg034201 vs. NCBI nr
Match: XP_022131300.1 (dihydrofolate reductase-like [Momordica charantia])

HSP 1 Score: 424.5 bits (1090), Expect = 6.6e-115
Identity = 211/256 (82.42%), Postives = 231/256 (90.23%), Query Frame = 0


Query: 1   MSSFSPSLCSHHQLLTPI--SIPRSPESDSQRISTMGSDGRIPRKPRFLCLHGFRTSGEI 60
           MSSFSP L S  QL TPI  S  RSP+   +R S MGS+ +I RKPRFLCLHGFRTSG+I
Sbjct: 1   MSSFSPPLRS-AQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQI 60

Query: 61  LKKQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGIFDPPYFEWFQFSAEFTEYRNFDEC 120
           LKKQVGKWP+SVLD FDL FLDAPFPAEGKSDVEGIFDPPYFEWFQFSA+  EY+NFDEC
Sbjct: 61  LKKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDEC 120

Query: 121 LSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTKVPKIKFVIIVSGAKFRSQ 180
           LSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ KG+ALTKVPKIKFV+IVSGAKFRS 
Sbjct: 121 LSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSP 180

Query: 181 SVAEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYVEPLVINHPKGHTVPRLDDKGLEV 240
           S+AEKAYS +IGCPSLHFLG+EDFL PHGL+LLESY+EPLVINHPKGHTVPRLD+KGL+V
Sbjct: 181 SLAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKV 240

Query: 241 MESFIERISKTMDETK 255
           MESFIERIS+T+ ET+
Sbjct: 241 MESFIERISQTLAETE 255

[Close]

BLAST of Spg034201 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 90.5 bits (223), Expect = 3.0e-17
Identity = 64/204 (31.37%), Postives = 94/204 (46.08%), Query Frame = 0


Query: 45  RFLCLHGFRTSGEILKKQVGKWPASVLDQFDLDFLDAPFPAEGKSD------------VE 104
           + LCLHG+  SG +  K++G     +    +L F   P  A+ ++D            + 
Sbjct: 6   KVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAALG 65

Query: 105 GIFDPPYFEWFQFSAEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ 164
           G  +   F WF+       Y ++DE L  I  YM + GPFDG +GFSQGA + A L  + 
Sbjct: 66  GEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQML 125

Query: 165 AKGV---ALTKVPKIKFVIIVSGAKFRSQSVA-EKAYSTAIGCPSLHFLGEEDFLKP--H 224
             G       + P  KFV+ V G  FR++    +  Y+  +  PSLH  G  D L P   
Sbjct: 126 QPGQPPNPYVQHPPFKFVVFVGG--FRAEKPEFDHFYNPKLTTPSLHIAGTSDTLVPLAR 185

Query: 225 GLELLESYVEPLVINHPKGHTVPR 231
             +L+E      V+ HP  H VP+
Sbjct: 186 SKQLVERCENAHVLLHPGQHIVPQ 207

[Close]

BLAST of Spg034201 vs. ExPASy Swiss-Prot
Match: Q0C7C4 (Esterase AAEL000016 OS=Aedes aegypti OX=7159 GN=AAEL000016 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.6e-15
Identity = 65/217 (29.95%), Postives = 105/217 (48.39%), Query Frame = 0


Query: 43  KPRFLCLHGFRTSGEILKKQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGIFDP-PYFE 102
           K + L LHG+R +G+  K ++G +   +    +L F+ AP  A    D E   +P P   
Sbjct: 24  KLKILALHGYRQNGDGFKSKLGSFRKFIGKHAELVFVTAPHIAPPLPDSEAGTEPDPAQR 83

Query: 103 WFQFSAEFTEYR---------NFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ 162
            + F+ +   ++          F++ L  +E  + K     G LGFSQGA     L  L 
Sbjct: 84  SWWFNKDDGTFKGTNKNGPAIGFEDSLKLVEK-VWKQEQCCGLLGFSQGACFVGLLCDLS 143

Query: 163 AKGVALTKVPKIKFVIIVSGAKFRSQSVAE-KAYSTAIGCPSLHFLGEEDFLKPH--GLE 222
           A+G  +T + K +F ++ SG  FRS S+     Y T +  PSLH  GE D + P    + 
Sbjct: 144 ARG--MTSI-KPEFAVLSSG--FRSGSLVHLNCYETKVQIPSLHIYGEADEIIPKEMSMA 203

Query: 223 LLESYVEPLVINHPKGHTVPRLDDKGLEVMESFIERI 247
           L +++ +P ++ HP GH +P    +    +E F ER+
Sbjct: 204 LADTFTDPQILTHPGGHFLPAQASQKQTYVEFFRERL 234

[Close]

BLAST of Spg034201 vs. ExPASy Swiss-Prot
Match: Q9VDL1 (Esterase CG5412 OS=Drosophila melanogaster OX=7227 GN=CG5412 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.7e-15
Identity = 66/224 (29.46%), Postives = 101/224 (45.09%), Query Frame = 0


Query: 40  IPRKPRFLCLHGFRTSGEILKKQVGKWPASVLDQFDLDFLDAPFPAEG-KSDVEGIFDPP 99
           I  K R LCLHG+R +GE  K ++G +        +  F+ AP  A+  +S  E +  P 
Sbjct: 27  ITEKVRVLCLHGYRQNGEAFKNKLGSFRKFANKYAEFVFITAPHVAKALESAAEPV--PE 86

Query: 100 YFEW--------FQFSAEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPG 159
              W        F+ + +      F E L  +E+     GPF G LGFSQGA     + G
Sbjct: 87  QRSWWANKDDGSFKGTNKGGPAFGFQESLRCVEEAWRTQGPFQGLLGFSQGACFVGLICG 146

Query: 160 LQAKGVALTKVPKIKFVIIVSGAKFRSQSVAE-KAYSTAIGCPSLHFLGEEDFLKPHGL- 219
           L  K   LT + + +F ++ SG  F S S+    AY  AI  P+LH  G+ D + P  + 
Sbjct: 147 LAKK--KLTSI-RPEFAVLASG--FLSGSLVHMSAYEEAISIPTLHIYGQTDEIIPKEMS 206

Query: 220 -ELLESYVEPLVINHPKGHTVPRLDDKGLEVMESFIERISKTMD 252
             L   +    V+ H  GH  P    +    +  F +R+ + ++
Sbjct: 207 ESLAARFKNAEVLEHSGGHYFPATAQQKQTFINFFQDRLQEYLE 243

[Close]

BLAST of Spg034201 vs. ExPASy Swiss-Prot
Match: Q3SZ07 (Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.4e-14
Identity = 63/204 (30.88%), Postives = 90/204 (44.12%), Query Frame = 0


Query: 45  RFLCLHGFRTSGEILKKQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGI---------F 104
           R LCL GFR S    +++ G    ++  + +L  L  P P    +  EG           
Sbjct: 8   RILCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVVDAAGSEGARPDSGPCPPE 67

Query: 105 DPPYFEWFQ------FSA--EFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAA 164
           + P   WF       F A  E T  R  +E L  +   + K GPFDG LGFSQGA L+A 
Sbjct: 68  EQPQGWWFSEQEADVFLALEEPTACRGLEEALETVAQALNKLGPFDGILGFSQGAALAAL 127

Query: 165 LPGLQAKGVALTKVPKIKFVIIVSGAKFRSQSVAEKAYSTAIGCPSLHFLGEEDFLKP-- 224
           +  L   G    + P  +FVI+VSG   R   + E      +  PSLH  G+ D + P  
Sbjct: 128 VCALGQGGD--PRFPLPRFVILVSGFCPRGLGLMEPIMQGPLSLPSLHVFGDTDGVIPSQ 187

Query: 225 HGLELLESYVEPLVINHPKGHTVP 230
             ++L   +   + + H  GH +P
Sbjct: 188 ESMQLCSRFDGAVTLTHSGGHFIP 209

[Close]

BLAST of Spg034201 vs. ExPASy Swiss-Prot
Match: Q94AC1 (Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=STR6 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.8e-14
Identity = 69/240 (28.75%), Postives = 104/240 (43.33%), Query Frame = 0


Query: 20  IPRSPESDSQRISTMGSDGRIPRKPRFLCLHGFRTSGEILKKQVGKWPASVLDQFDLDFL 79
           +P S +  +Q   + G + R  RK R LCLHGFR +    K + G     + +  +L F+
Sbjct: 332 VPLSLDPLNQPSESNGDNTR--RKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFI 391

Query: 80  DAPF----------PAEGKSD------VEGIFD-PPYFEWFQFSAEF------TEYRNFD 139
           DAP           P  G  +      V   FD P    W     +F      T+   FD
Sbjct: 392 DAPHELQFIYQTATPPSGVCNKKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFD 451

Query: 140 ECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTKVPKIKFVIIVSGAKFR 199
           + L++++    + GPFDG LGFSQGA ++AA+ G Q +   L      +F ++ SG  F 
Sbjct: 452 KSLTYLKTAFEEKGPFDGILGFSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FT 511

Query: 200 SQSVAEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYVEPL-------VINHPKGHTVP 230
              + E     +I CPSLH  G +       +    S +  L       ++ H  GH +P
Sbjct: 512 PWPLLEMKEKRSIKCPSLHIFGSQPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564

[Close]

BLAST of Spg034201 vs. ExPASy TrEMBL
Match: A0A6J1K928 (dihydrofolate reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492210 PE=4 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 5.6e-120
Identity = 216/255 (84.71%), Postives = 230/255 (90.20%), Query Frame = 0


Query: 1   MSSFSPSLCSHHQLLTPISIPRSPESDSQRISTMGSDGRIPRKPRFLCLHGFRTSGEILK 60
           MSS SP LCS      PIS  RSPE   QRI  MG+DG I RKPRFLCLHGFRTSG ILK
Sbjct: 1   MSSLSPLLCS------PISKTRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILK 60

Query: 61  KQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGIFDPPYFEWFQFSAEFTEYRNFDECLS 120
           KQVGKWP SVL+Q DLDFLDAPFPAEGKSD+EGIFDPPYFEWFQFS EFTEYRNFDECLS
Sbjct: 61  KQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLS 120

Query: 121 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTKVPKIKFVIIVSGAKFRSQSV 180
           FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKG+ALTKVPKIKFVIIVSGAKFRSQSV
Sbjct: 121 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGMALTKVPKIKFVIIVSGAKFRSQSV 180

Query: 181 AEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYVEPLVINHPKGHTVPRLDDKGLEVME 240
           AEKAYS+AIGCPSLHFLG+EDFL PHG +LLESY+EP+VINHPKGHT+PRLDD+GLEVME
Sbjct: 181 AEKAYSSAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDRGLEVME 240

Query: 241 SFIERISKTMDETKE 256
           SFIE+ISK +DE +E
Sbjct: 241 SFIEKISKMLDEAEE 249

[Close]

BLAST of Spg034201 vs. ExPASy TrEMBL
Match: A0A6J1HF66 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462484 PE=4 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 2.8e-119
Identity = 214/255 (83.92%), Postives = 228/255 (89.41%), Query Frame = 0


Query: 1   MSSFSPSLCSHHQLLTPISIPRSPESDSQRISTMGSDGRIPRKPRFLCLHGFRTSGEILK 60
           MSS SP LCS      PIS  RSPE   QRI  MG+DG I RKPRFLCLHGFRTSG ILK
Sbjct: 1   MSSLSPLLCS------PISKSRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILK 60

Query: 61  KQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGIFDPPYFEWFQFSAEFTEYRNFDECLS 120
           KQVGKWP SVL+Q DLDFLDAPFPAEGKSD+EGIFDPPYFEWFQFS EFTEYRNFDECLS
Sbjct: 61  KQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLS 120

Query: 121 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTKVPKIKFVIIVSGAKFRSQSV 180
           FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKG+ALTKVPKIKFVIIVSGAKFRSQSV
Sbjct: 121 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSV 180

Query: 181 AEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYVEPLVINHPKGHTVPRLDDKGLEVME 240
           AEKAYS  IGCPSLHFLG+EDFL PHG +LLESY+EP++INHPKGHT+PRLDD+GLEVME
Sbjct: 181 AEKAYSPVIGCPSLHFLGKEDFLMPHGQKLLESYIEPVIINHPKGHTIPRLDDEGLEVME 240

Query: 241 SFIERISKTMDETKE 256
           SFIE+ISK +DE +E
Sbjct: 241 SFIEKISKMLDEAEE 249

[Close]

BLAST of Spg034201 vs. ExPASy TrEMBL
Match: A0A6J1BPA7 (dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111004563 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 3.2e-115
Identity = 211/256 (82.42%), Postives = 231/256 (90.23%), Query Frame = 0


Query: 1   MSSFSPSLCSHHQLLTPI--SIPRSPESDSQRISTMGSDGRIPRKPRFLCLHGFRTSGEI 60
           MSSFSP L S  QL TPI  S  RSP+   +R S MGS+ +I RKPRFLCLHGFRTSG+I
Sbjct: 1   MSSFSPPLRS-AQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQI 60

Query: 61  LKKQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGIFDPPYFEWFQFSAEFTEYRNFDEC 120
           LKKQVGKWP+SVLD FDL FLDAPFPAEGKSDVEGIFDPPYFEWFQFSA+  EY+NFDEC
Sbjct: 61  LKKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDEC 120

Query: 121 LSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTKVPKIKFVIIVSGAKFRSQ 180
           LSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ KG+ALTKVPKIKFV+IVSGAKFRS 
Sbjct: 121 LSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSP 180

Query: 181 SVAEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYVEPLVINHPKGHTVPRLDDKGLEV 240
           S+AEKAYS +IGCPSLHFLG+EDFL PHGL+LLESY+EPLVINHPKGHTVPRLD+KGL+V
Sbjct: 181 SLAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKV 240

Query: 241 MESFIERISKTMDETK 255
           MESFIERIS+T+ ET+
Sbjct: 241 MESFIERISQTLAETE 255

[Close]

BLAST of Spg034201 vs. ExPASy TrEMBL
Match: A0A1S3BC97 (dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.3e-111
Identity = 203/255 (79.61%), Postives = 219/255 (85.88%), Query Frame = 0


Query: 1   MSSFSPSLCSHHQLLTPISIPRSPESDSQRISTMGSDGRIPRKPRFLCLHGFRTSGEILK 60
           MSSF PSL SH  L T       P+     +  MGSD +I  KPRFLCLHGFRTS  ILK
Sbjct: 1   MSSFFPSLSSHQFLST------IPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILK 60

Query: 61  KQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGIFDPPYFEWFQFSAEFTEYRNFDECLS 120
           KQVGKWP SVLDQ DL FLDAPFPAEGKSDVEGIFDPPYFEWFQFS EFTEYRNFDECLS
Sbjct: 61  KQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLS 120

Query: 121 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTKVPKIKFVIIVSGAKFRSQSV 180
           FIE+YM++HGPFDGFLGFSQGAILSAALPG QAKG+ALTKVPKIKFVIIVSGAKFRS+SV
Sbjct: 121 FIENYMIEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESV 180

Query: 181 AEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYVEPLVINHPKGHTVPRLDDKGLEVME 240
           AEKAYST IGCPSLHFLGEEDFL PHG +LLESY+EP +I HPKGHT+PRLDDK LEVME
Sbjct: 181 AEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVME 240

Query: 241 SFIERISKTMDETKE 256
           SFI+RISK ++ET+E
Sbjct: 241 SFIQRISKMLNETEE 249

[Close]

BLAST of Spg034201 vs. ExPASy TrEMBL
Match: A0A0A0LNX4 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 4.0e-110
Identity = 201/255 (78.82%), Postives = 214/255 (83.92%), Query Frame = 0


Query: 1   MSSFSPSLCSHHQLLTPISIPRSPESDSQRISTMGSDGRIPRKPRFLCLHGFRTSGEILK 60
           M  F PSL SH  L T       P+     I  MGSD +   KPRFLCLHGFRTS  ILK
Sbjct: 1   MPPFFPSLSSHQSLTT------IPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILK 60

Query: 61  KQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGIFDPPYFEWFQFSAEFTEYRNFDECLS 120
           KQVGKWP SVLDQ DL FLDAPFPAEGKSDVEGIFDPPYFEWFQFS EFTEYRNFDECLS
Sbjct: 61  KQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLS 120

Query: 121 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTKVPKIKFVIIVSGAKFRSQSV 180
           FIE+YM+KHGPFDGFLGFSQGAILSAALPG QAKG+ALTKVPKIKFVIIVSGAKFRS+SV
Sbjct: 121 FIENYMIKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESV 180

Query: 181 AEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYVEPLVINHPKGHTVPRLDDKGLEVME 240
           AEKAYST IGCPSLHFLGEEDFL PHG +LLESY+EP +I HPKGHT+PRLDDK LEVME
Sbjct: 181 AEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVME 240

Query: 241 SFIERISKTMDETKE 256
           SFI RISK ++E +E
Sbjct: 241 SFIHRISKILNENEE 249

[Close]

BLAST of Spg034201 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 320.9 bits (821), Expect = 9.5e-88
Identity = 151/223 (67.71%), Postives = 184/223 (82.51%), Query Frame = 0


Query: 34  MGSDGR-IPRKPRFLCLHGFRTSGEILKKQVGKWPASVLDQFDLDFLDAPFPAEGKSDVE 93
           MGS+GR I RKPRFLCLHGFRTSGEI+K Q+ KWP SV+D+ DL FLDAPFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 94  GIFDPPYFEWFQFSAEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ 153
           GIFDPPY+EWFQF+ EFTEY NF++CL ++ED M+K GPFDG +GFSQGAILS  LPGLQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 154 AKGVALTKVPKIKFVIIVSGAKFRSQSVAEKAYSTAIGCPSLHFLGEEDFLKPHGLELLE 213
           AKG+A  KVPKIKFVII+ GAK +S  +AE AYS+++   SLHFLGE DFLKP+G +L+E
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGETDFLKPYGTQLIE 180

Query: 214 SYVEPLVINHPKGHTVPRLDDKGLEVMESFIERISK-TMDETK 255
           SY  P+V++HPKGHTVPRLD+K LE + +FI+ +    M+E K
Sbjct: 181 SYKNPVVVHHPKGHTVPRLDEKSLEKVTAFIDTLEHLVMEEDK 223

[Close]

BLAST of Spg034201 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 282.7 bits (722), Expect = 2.9e-76
Identity = 130/217 (59.91%), Postives = 165/217 (76.04%), Query Frame = 0


Query: 41  PRKPRFLCLHGFRTSGEILKKQVGKWPASVLDQFDLDFLDAPFPAEGKSDVEGIFDPPYF 100
           P+ PR LCLHGFRTSG IL+  +GKWP ++L   DLDFLDAPFPA GKSDVE  FDPPY+
Sbjct: 27  PKNPRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVERFFDPPYY 86

Query: 101 EWFQFSAEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTK 160
           EW+Q +  F EYRNF+ECL++IEDYM+K+GPFDG LGFSQGA L+AA+PG+Q +G ALTK
Sbjct: 87  EWYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGSALTK 146

Query: 161 VPKIKFVIIVSGAK-----FRSQSVAEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYV 220
           VPK+KF++I+SGAK     F     A  A+S+ + CPSLHF+GE DFLK  G  L+ES+V
Sbjct: 147 VPKVKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFIGERDFLKIEGEVLVESFV 206

Query: 221 EPLVINHPKGHTVPRLDDKGLEVMESFIERISKTMDE 253
           EP+VI+H  GH +P+LD K  E M SF + I + + +
Sbjct: 207 EPVVIHHSGGHIIPKLDTKAEETMLSFFQSIRQMLSD 243

[Close]

BLAST of Spg034201 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 253.8 bits (647), Expect = 1.4e-67
Identity = 119/166 (71.69%), Postives = 140/166 (84.34%), Query Frame = 0


Query: 34  MGSDGR-IPRKPRFLCLHGFRTSGEILKKQVGKWPASVLDQFDLDFLDAPFPAEGKSDVE 93
           MGS+GR I RKPRFLCLHGFRTSGEI+K Q+ KWP SV+D+ DL FLDAPFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 94  GIFDPPYFEWFQFSAEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ 153
           GIFDPPY+EWFQF+ EFTEY NF++CL ++ED M+K GPFDG +GFSQGAILS  LPGLQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 154 AKGVALTKVPKIKFVIIVSGAKFRSQSVAEKAYSTAIGCPSLHFLG 199
           AKG+A  KVPKIKFVII+ GAK +S  +AE AYS+++   SLHFLG
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLG 166

[Close]

BLAST of Spg034201 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-15
Identity = 69/240 (28.75%), Postives = 104/240 (43.33%), Query Frame = 0


Query: 20  IPRSPESDSQRISTMGSDGRIPRKPRFLCLHGFRTSGEILKKQVGKWPASVLDQFDLDFL 79
           +P S +  +Q   + G + R  RK R LCLHGFR +    K + G     + +  +L F+
Sbjct: 332 VPLSLDPLNQPSESNGDNTR--RKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFI 391

Query: 80  DAPF----------PAEGKSD------VEGIFD-PPYFEWFQFSAEF------TEYRNFD 139
           DAP           P  G  +      V   FD P    W     +F      T+   FD
Sbjct: 392 DAPHELQFIYQTATPPSGVCNKKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFD 451

Query: 140 ECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGVALTKVPKIKFVIIVSGAKFR 199
           + L++++    + GPFDG LGFSQGA ++AA+ G Q +   L      +F ++ SG  F 
Sbjct: 452 KSLTYLKTAFEEKGPFDGILGFSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FT 511

Query: 200 SQSVAEKAYSTAIGCPSLHFLGEEDFLKPHGLELLESYVEPL-------VINHPKGHTVP 230
              + E     +I CPSLH  G +       +    S +  L       ++ H  GH +P
Sbjct: 512 PWPLLEMKEKRSIKCPSLHIFGSQPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564

The following BLAST results are available for this feature:

BLAST of Spg034201 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023545459.1	5.2e-120	85.10	dihydrofolate reductase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022997250.1	1.2e-119	84.71	dihydrofolate reductase-like [Cucurbita maxima]	[more]
XP_022961839.1	5.8e-119	83.92	dihydrofolate reductase-like [Cucurbita moschata]	[more]
XP_038885360.1	6.6e-115	89.47	dihydrofolate reductase-like [Benincasa hispida]	[more]
XP_022131300.1	6.6e-115	82.42	dihydrofolate reductase-like [Momordica charantia]	[more]

BLAST of Spg034201 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P36591	3.0e-17	31.37	Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O...	[more]
Q0C7C4	1.6e-15	29.95	Esterase AAEL000016 OS=Aedes aegypti OX=7159 GN=AAEL000016 PE=3 SV=1	[more]
Q9VDL1	4.7e-15	29.46	Esterase CG5412 OS=Drosophila melanogaster OX=7227 GN=CG5412 PE=2 SV=1	[more]
Q3SZ07	1.4e-14	30.88	Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1	[more]
Q94AC1	1.8e-14	28.75	Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=ST...	[more]

BLAST of Spg034201 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K928	5.6e-120	84.71	dihydrofolate reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492210 PE=4 SV...	[more]
A0A6J1HF66	2.8e-119	83.92	dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462484 PE=4 ...	[more]
A0A6J1BPA7	3.2e-115	82.42	dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111004563 PE=4...	[more]
A0A1S3BC97	1.3e-111	79.61	dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1	[more]
A0A0A0LNX4	4.0e-110	78.82	FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 S...	[more]

BLAST of Spg034201 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24380.1	9.5e-88	67.71	INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and deri...	[more]
AT5G65400.1	2.9e-76	59.91	alpha/beta-Hydrolases superfamily protein	[more]
AT4G24380.2	1.4e-67	71.69	CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidop...	[more]
AT1G09280.1	1.3e-15	28.75	CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolas...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005645	Serine hydrolase FSH	PFAM	PF03959	FSH1	coord: 43..233 e-value: 6.5E-49 score: 166.5
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 40..251 e-value: 1.9E-49 score: 170.3
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 41..229
None	No IPR available	PANTHER	PTHR48070	ESTERASE OVCA2	coord: 39..253
None	No IPR available	PANTHER	PTHR48070:SF5	DIHYDROFOLATE REDUCTASE	coord: 39..253

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg034201.1	Spg034201.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004146	dihydrofolate reductase activity