Homology
BLAST of Spg034115 vs. NCBI nr
Match:
XP_023546149.1 (probable ubiquitin-like-specific protease 2B isoform X3 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1409.4 bits (3647), Expect = 0.0e+00
Identity = 761/1065 (71.46%), Postives = 808/1065 (75.87%), Query Frame = 0
Query: 1 MKNAAVKGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLEC---------- 60
MKNA+VKGLEVFDFTEEDELPELISEKRLSKFKNPN++TN VLKYEFLEC
Sbjct: 1 MKNASVKGLEVFDFTEEDELPELISEKRLSKFKNPNLDTNAVLKYEFLECDKLTVANGFV 60
Query: 61 --GKEIENPHMDVDLDECNRGCDDGISHIPSGSTKEQFIMKEEKYQLDANTESELNSHSQ 120
GKEI+NPHMDVDL +CNRGCD+GISHIPSG+TKEQFIM+E KYQLDANTESEL SHSQ
Sbjct: 61 VEGKEIKNPHMDVDLGKCNRGCDNGISHIPSGTTKEQFIMEEGKYQLDANTESELKSHSQ 120
Query: 121 DVFMQVDNHVTGSLGSDSELGKIGSSSQSPTLGLNCTLPEFATERKQVDAISDPNGSMNG 180
D+F+QVD HVTGSLG SEL KIGSSSQSP LGLNCTLPEF ER+ +DA+SDPNGSMNG
Sbjct: 121 DMFVQVDGHVTGSLG--SELDKIGSSSQSPILGLNCTLPEFNAEREPIDALSDPNGSMNG 180
Query: 181 SSPMSSPSEIVEDGVLLNGKSSDKCSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFS 240
+S MS PSE VEDGVLLNGK D CSSDNEMDDLNKEVVL PDY+VYGDFYCASP LTFS
Sbjct: 181 NSSMSPPSETVEDGVLLNGKYLDNCSSDNEMDDLNKEVVLYPDYIVYGDFYCASPSLTFS 240
Query: 241 NNGIKINGFAEYGSDEFLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACD 300
N IKINGFA+YGS+EFLNLEWRVDDLIDIECQ FQRVEYVMIKLHVISKDAGQCDN+CD
Sbjct: 241 PNAIKINGFADYGSNEFLNLEWRVDDLIDIECQCFQRVEYVMIKLHVISKDAGQCDNSCD 300
Query: 301 TSGQWTSASIKEVKIALVESHWSEKQQKIRSLDSRYMAIWNMSLFAGIGTDDDDLGGQRH 360
TSG IKEVKI LV+S+WSEKQQKIRSLDSRYMAIWNMSL AGIGTDDDDLGGQRH
Sbjct: 301 TSG------IKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNMSLDAGIGTDDDDLGGQRH 360
Query: 361 YFPYFDEHFEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKH 420
YFP FDE FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLK QIDP++KH
Sbjct: 361 YFPNFDEPFEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPQDKH 420
Query: 421 RFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSL 480
RFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLF+KDYIFIPINF+LHWSL
Sbjct: 421 RFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFEKDYIFIPINFNLHWSL 480
Query: 481 MVICHPGEVARYSDEDLMKSMKVPCILHMDSIKGSHAAGLATLLAMEHQLYSGLTLGLPT 540
MVICHPGEVARYSDEDL KSMKVPCILHMDSIKGSHA GL
Sbjct: 481 MVICHPGEVARYSDEDLTKSMKVPCILHMDSIKGSHA-------------------GLKN 540
Query: 541 LLWPLGIPTYTVFHKAKRRQLKRFGIFKDEEAIKWADLSLDVAPIVLSHEEDSWGWNIHP 600
L+
Sbjct: 541 LI---------------------------------------------------------- 600
Query: 601 SRQFSTKSLLSDMSDSRCLLESSLTKGIWKDNYPKKIKFFLWEISHRAISTKDVLQKRMP 660
Sbjct: 601 ------------------------------------------------------------ 660
Query: 661 YLAISPDENSVPSYKRSYLLEEWKERNKETSEDISSKFKNLRFLPLELNVDWFPPAEAYL 720
+SYLLEEWKERNKET EDIS+KFKNLRFLPLELNVDWFPPAEAYL
Sbjct: 661 ---------------QSYLLEEWKERNKETPEDISTKFKNLRFLPLELNVDWFPPAEAYL 720
Query: 721 KRTLIQRLIFEILENRSREMSTAACSDELLSKFPSNNEDETGVELLPERGSPAVTCNHNL 780
KRTLIQRLIFEILENRSREMSTAACSDELLSKFPSNNEDETGVELL ERGSPAV CNHNL
Sbjct: 721 KRTLIQRLIFEILENRSREMSTAACSDELLSKFPSNNEDETGVELLLERGSPAVACNHNL 780
Query: 781 SSSQAVDGIEITLLSESSSRHNHFLDGSGLVVRELFEPGASNGSLLGHYQSFAQTSSYFD 840
SSSQAVDGIEITLLSESSSR NHF+DGSGLVVRELFEPGASNGSLLG YQSFAQTSSYFD
Sbjct: 781 SSSQAVDGIEITLLSESSSRPNHFIDGSGLVVRELFEPGASNGSLLGDYQSFAQTSSYFD 840
Query: 841 ANGTVLEEDADAETEDRFMYLPPEQDGLQPTDAMTSQACRFPCSSRGLESEAAFDLCMSI 900
N TVLE+DADAET DRFMYLPP QDGLQP DAM SQACRFPCSS GLES+A+FDLCMSI
Sbjct: 841 TNATVLEDDADAETGDRFMYLPPVQDGLQPIDAMASQACRFPCSSIGLESKASFDLCMSI 894
Query: 901 QPEHGGGSASSPSSHADHLADVGIIESCDVREASPGNKEEMSRKRPLS--TENLEQPVAE 960
QPE G G A SS A+ L DVGIIESC VREASPGNKEE +RKRPLS ENLE PVAE
Sbjct: 901 QPERGRGIA---SSSAEDLEDVGIIESCKVREASPGNKEERNRKRPLSHEKENLE-PVAE 894
Query: 961 CPTSPAATMLDVDAIVISQDANAICEDMDNNGSDPP-CKETLVASPRLDEDITTKADIKH 1020
PTS A T+ DVD IVISQD N M +N S+PP C++TL+ASP LDE TK D++H
Sbjct: 961 RPTSAATTVQDVDTIVISQDTN-----MGSNDSEPPLCEDTLIASPCLDE--ATKTDVEH 894
Query: 1021 DDALVVASVVEVDLDEQPAAKKPRHSLYLEEPSNGLKEGLSDDAD 1051
DD+ VV S+ EV+LDE+P AKKPRHS Y EE S ++EGLSD AD
Sbjct: 1021 DDSPVVVSITEVNLDEEPPAKKPRHSPYPEETSKSVEEGLSDGAD 894
BLAST of Spg034115 vs. NCBI nr
Match:
XP_022962129.1 (probable ubiquitin-like-specific protease 2B isoform X3 [Cucurbita moschata])
HSP 1 Score: 1406.3 bits (3639), Expect = 0.0e+00
Identity = 761/1066 (71.39%), Postives = 808/1066 (75.80%), Query Frame = 0
Query: 1 MKNAAVKGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLEC---------- 60
MKNA+VKGLEVFDFTEEDELPELISEKRLSKFKNPN++TN VLKYEFLEC
Sbjct: 1 MKNASVKGLEVFDFTEEDELPELISEKRLSKFKNPNLDTNAVLKYEFLECDKLTVANGFV 60
Query: 61 --GKEIENPHMDVDLDECNRGCDDGISHIPSGSTKEQFIMKEEKYQLDANTESELNSHSQ 120
GKEI+NPHMDVDL +CNRGCD+GISHIPSG+TKEQFIM+E KYQLDANTESELNSHSQ
Sbjct: 61 VEGKEIKNPHMDVDLGKCNRGCDNGISHIPSGTTKEQFIMEEGKYQLDANTESELNSHSQ 120
Query: 121 DVFMQVDNHVTGSLGSDSELGKIGSSSQSPTLGLNCTLPEFATERKQVDAISDPNGSMNG 180
D+F+QVD HVTGSLG SEL KIGSSSQSP LGLNCTLPEF ER+ +DA+SDPNGSMNG
Sbjct: 121 DLFVQVDGHVTGSLG--SELDKIGSSSQSPILGLNCTLPEFNAEREPIDALSDPNGSMNG 180
Query: 181 SSPMSSPSEIVEDGVLLNGKSSDKCSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFS 240
+S MS PSE VEDGVLLNGK D CSSDNEMDDLNKEVVL PDY+VYGDFYCASP LTFS
Sbjct: 181 NSSMSPPSETVEDGVLLNGKYLDNCSSDNEMDDLNKEVVLYPDYIVYGDFYCASPSLTFS 240
Query: 241 NNGIKINGFAEYGSDEFLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACD 300
N IKINGFA+YGS+EFLNLEWRVDDLIDIECQ FQRVEYVMIKLHVISKDAGQCDN+CD
Sbjct: 241 PNAIKINGFADYGSNEFLNLEWRVDDLIDIECQCFQRVEYVMIKLHVISKDAGQCDNSCD 300
Query: 301 TSGQWTSASIKEVKIALVESHWSEKQQKIRSLDSRYMAIWNMSLFAGIGTDDDDLGGQRH 360
TSG IKEVKI LV+S+WSEKQQKIRSLDSRYMAIWNMSL AGIGTDDDDLGGQRH
Sbjct: 301 TSG------IKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNMSLDAGIGTDDDDLGGQRH 360
Query: 361 YFPYFDEHFEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKH 420
YFP FDE FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLK QIDP++KH
Sbjct: 361 YFPNFDEPFEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPQDKH 420
Query: 421 RFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSL 480
RFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLF+KDYIFIPINF+LHWSL
Sbjct: 421 RFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFEKDYIFIPINFNLHWSL 480
Query: 481 MVICHPGEVARYSDEDLMKSMKVPCILHMDSIKGSHAAGLATLLAMEHQLYSGLTLGLPT 540
MVICH GEVARYSDEDL KSMKVPCILHMDSIKGSHA GL
Sbjct: 481 MVICHLGEVARYSDEDLTKSMKVPCILHMDSIKGSHA-------------------GLKN 540
Query: 541 LLWPLGIPTYTVFHKAKRRQLKRFGIFKDEEAIKWADLSLDVAPIVLSHEEDSWGWNIHP 600
L+
Sbjct: 541 LI---------------------------------------------------------- 600
Query: 601 SRQFSTKSLLSDMSDSRCLLESSLTKGIWKDNYPKKIKFFLWEISHRAISTKDVLQKRMP 660
Sbjct: 601 ------------------------------------------------------------ 660
Query: 661 YLAISPDENSVPSYKRSYLLEEWKERNKETSEDISSKFKNLRFLPLELNVDWFPPAEAYL 720
+SYLLEEWKERNKET EDIS+KFKNLRFLPLELNVDWFPPAEAYL
Sbjct: 661 ---------------QSYLLEEWKERNKETPEDISTKFKNLRFLPLELNVDWFPPAEAYL 720
Query: 721 KRTLIQRLIFEILENRSREMSTAACSDELLSKFPSNNEDETGVELLPERGSPAVTCNHNL 780
KRTLIQRLIFEILENRSREMSTAACSDELLSKFPSNNEDETGVELL ERGSPAV CNHNL
Sbjct: 721 KRTLIQRLIFEILENRSREMSTAACSDELLSKFPSNNEDETGVELLLERGSPAVACNHNL 780
Query: 781 SSSQAVDGIEITLLSESSSRHNHFLDGSGLVVRELFEPGASNGSLLGHYQSFAQTSSYFD 840
SSSQAVDGIEITLLSESSSR NHF+DGSGLVVRELFEPGASNGSLLG YQSFAQTSSYFD
Sbjct: 781 SSSQAVDGIEITLLSESSSRPNHFIDGSGLVVRELFEPGASNGSLLGDYQSFAQTSSYFD 840
Query: 841 ANGTVLEEDADAETEDRFMYLPPEQDGLQPTDAMTSQACRFPCSSRGLESEAAFDLCMSI 900
N TVLE+DADAET DRFM+LPP QDGLQP DAM SQACRFPCSS GLES+A+FDLCMSI
Sbjct: 841 TNATVLEDDADAETGDRFMFLPPVQDGLQPIDAMASQACRFPCSSIGLESKASFDLCMSI 895
Query: 901 QPEHGGGSASSPSSHADHLADVGIIESCDVREASPGNKEEMSRKRPLS--TENLEQPVAE 960
QPE G G A SS A+ L DVGIIESCDVREASPGNKE+ +RKRPLS ENLE PV E
Sbjct: 901 QPERGRGIA---SSSAEDLEDVGIIESCDVREASPGNKEDRNRKRPLSHEQENLE-PVTE 895
Query: 961 CPTSPAATMLDVDAIVISQDANAICEDMDNNGSDPPCK-ETLVASPRLDEDITTKADIKH 1020
PTS A T+ DVD IVISQD N M +N S+P C+ +TLVASP LDE TK D++H
Sbjct: 961 RPTSAATTVQDVDTIVISQDTN-----MGSNDSEPLCEDDTLVASPCLDE--ATKTDVEH 895
Query: 1021 DDALVVASVVEVDLDEQPAAKKPRHSLYLEEPSNGLKEGLSDDADL 1052
DD VV S+ EV+LDE+P AKKPRHS Y EE SNG+ EGLSD AD+
Sbjct: 1021 DDLPVVVSITEVNLDEEPPAKKPRHSPYPEETSNGVGEGLSDGADI 895
BLAST of Spg034115 vs. NCBI nr
Match:
XP_022951344.1 (probable ubiquitin-like-specific protease 2B isoform X2 [Cucurbita moschata])
HSP 1 Score: 1400.2 bits (3623), Expect = 0.0e+00
Identity = 739/1051 (70.31%), Postives = 793/1051 (75.45%), Query Frame = 0
Query: 1 MKNAAVKGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLECGKEIENPHMD 60
M +A+VKGLEVFDFTEEDELPELISEK LSKFKNPN+ETN VLKY LE GKE+ENPHMD
Sbjct: 1 MNDASVKGLEVFDFTEEDELPELISEKCLSKFKNPNLETNSVLKYGCLELGKEVENPHMD 60
Query: 61 VDLDECNRGCDDGISHIPSGSTKEQFIMKEEKYQLDANTESELNSHSQDVFMQVDNHVTG 120
VDLDECNRGCD+GISHIP G +KEQ IM+EEKYQLD NTESE N+HSQD+F+QV+NHVTG
Sbjct: 61 VDLDECNRGCDNGISHIPPGISKEQLIMEEEKYQLDVNTESEWNTHSQDMFVQVNNHVTG 120
Query: 121 SLGSDSELGKIGSSSQSPTLGLNCTLPEFATERKQVDAISDPNGSMNGSSPMSSPSEIVE 180
G SELGK+GSSSQS LGLNC+LPEFA +R+QVDA+S PNGSMNGSSPMSSPSE+VE
Sbjct: 121 CFG--SELGKVGSSSQSSILGLNCSLPEFAAKREQVDALSYPNGSMNGSSPMSSPSELVE 180
Query: 181 DGVLLNGKSSDKCSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFSNNGIKINGFAEY 240
D V NGKSSDKCSSDNEMDDLN+EVVLCPDY++YGD YC S LTFS+NG+KINGF++Y
Sbjct: 181 DSVSPNGKSSDKCSSDNEMDDLNQEVVLCPDYIIYGDSYCTSSQLTFSHNGVKINGFSDY 240
Query: 241 GSDEFLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACDTSGQWTSASIKE 300
GS+EFLNLEW VDDLI+IEC WFQRVE+V IKLH ISKD GQ DN CDTSG +KE
Sbjct: 241 GSNEFLNLEWGVDDLINIECHWFQRVEFVTIKLHTISKDVGQRDNGCDTSG------LKE 300
Query: 301 VKIALVESHWSEKQQKIRSLDSRYMAIWNMSLFAGIGTDDDDLGGQRHYFPYFDEHFEEV 360
VKI LV+ WSEKQQKIRSLDSRYMAIWNMSL G DDDLGG R YFP FDEHFEEV
Sbjct: 301 VKIVLVDPCWSEKQQKIRSLDSRYMAIWNMSLDVG----DDDLGGPRQYFPNFDEHFEEV 360
Query: 361 VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKHRFHFFNSFFFRK 420
VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLK QIDPKEKHRFHFFNSFFFRK
Sbjct: 361 VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPKEKHRFHFFNSFFFRK 420
Query: 421 LADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHPGEVARY 480
LADLDKDPSSASDG+AAFLRVRKWTRKV+LFDKDYIFIPINF+LHWSLMVICHPGEVARY
Sbjct: 421 LADLDKDPSSASDGKAAFLRVRKWTRKVNLFDKDYIFIPINFNLHWSLMVICHPGEVARY 480
Query: 481 SDEDLMKSMKVPCILHMDSIKGSHAAGLATLLAMEHQLYSGLTLGLPTLLWPLGIPTYTV 540
SDE+LMKS KVPCILHMDSIKGSHA GL L+
Sbjct: 481 SDENLMKSTKVPCILHMDSIKGSHA-------------------GLKNLI---------- 540
Query: 541 FHKAKRRQLKRFGIFKDEEAIKWADLSLDVAPIVLSHEEDSWGWNIHPSRQFSTKSLLSD 600
Sbjct: 541 ------------------------------------------------------------ 600
Query: 601 MSDSRCLLESSLTKGIWKDNYPKKIKFFLWEISHRAISTKDVLQKRMPYLAISPDENSVP 660
Sbjct: 601 ------------------------------------------------------------ 660
Query: 661 SYKRSYLLEEWKERNKETSEDISSKFKNLRFLPLELNVDWFPPAEAYLKRTLIQRLIFEI 720
+SYLLEEW ERNK+ SEDISSKFKNLRFLPLELNVDWFPPAEAYLKRTLIQRLIFEI
Sbjct: 661 ---QSYLLEEWNERNKDASEDISSKFKNLRFLPLELNVDWFPPAEAYLKRTLIQRLIFEI 720
Query: 721 LENRSREMSTAACSDELLSKFPSNNEDETGVELLPERGSPAVTCNHNLSSSQAVDGIEIT 780
LE RSR+MST ACSD+LLSKFPSNNEDE GVEL+PE G A T NHNLSSSQA DGIEIT
Sbjct: 721 LEKRSRQMSTTACSDDLLSKFPSNNEDEAGVELVPESGRLAETFNHNLSSSQAADGIEIT 780
Query: 781 LLSESSSRHNHFLDGSGLVVRELFEPGASNGSLLGHYQSFAQTSSYFDANGTVLEEDADA 840
LLSESSSRHNHF+DGSGLVVRELFEPG SNGSLLGHYQSFAQTSSYFD N TVLEEDAD
Sbjct: 781 LLSESSSRHNHFMDGSGLVVRELFEPGTSNGSLLGHYQSFAQTSSYFDTNVTVLEEDADT 840
Query: 841 ETEDRFMYLPPEQDGLQPTDAMTSQACRFPCSSRGLESEAAFDLCMSIQPEHGGGSASSP 900
ET DRFMYLP EQDGLQP DAMTSQACRFP SSRGLESE AFDLCMSIQPEHG G ASSP
Sbjct: 841 ETGDRFMYLPSEQDGLQPVDAMTSQACRFPGSSRGLESETAFDLCMSIQPEHGSGIASSP 886
Query: 901 SSHADHLADVGIIESCDVREASPGNKEEMSRKRPLSTENLEQPVAECPTSPAATMLDVDA 960
SSH+D L DVGIIESCDVRE SPGNKEE++RKRPL ENLE P+AECPTS A T D D
Sbjct: 901 SSHSDVLEDVGIIESCDVREPSPGNKEEINRKRPLPIENLE-PIAECPTSAATTTQDTDT 886
Query: 961 IVISQDANAICEDMDNNGSDPPCKETLVASPRLDEDITTKADIKHDDALVVASVVEVDLD 1020
IV+S+D N CEDM+N+GSDP CKET+VASP LDEDITTK DI+HDDA++VASV EVDL
Sbjct: 961 IVVSKDTNDTCEDMENDGSDPHCKETVVASPCLDEDITTKTDIEHDDAVLVASVTEVDLH 886
Query: 1021 EQPAAKKPRHSLYLEEPSNGLKEGLSDDADL 1052
EQP AKK RH Y EE SNGL + L +DADL
Sbjct: 1021 EQPPAKKSRHLPYPEEASNGLGDVLLEDADL 886
BLAST of Spg034115 vs. NCBI nr
Match:
XP_023546148.1 (probable ubiquitin-like-specific protease 2B isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1398.6 bits (3619), Expect = 0.0e+00
Identity = 761/1093 (69.62%), Postives = 808/1093 (73.92%), Query Frame = 0
Query: 1 MKNAAVKGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLECGKEIENPHMD 60
MKNA+VKGLEVFDFTEEDELPELISEKRLSKFKNPN++TN VLKYEFLECGKEI+NPHMD
Sbjct: 1 MKNASVKGLEVFDFTEEDELPELISEKRLSKFKNPNLDTNAVLKYEFLECGKEIKNPHMD 60
Query: 61 VDLDECNRGCDDGISHIPSGSTKEQFIMKEEKYQLDANTESELNSHSQDVFMQVDNHVTG 120
VDL +CNRGCD+GISHIPSG+TKEQFIM+E KYQLDANTESEL SHSQD+F+QVD HVTG
Sbjct: 61 VDLGKCNRGCDNGISHIPSGTTKEQFIMEEGKYQLDANTESELKSHSQDMFVQVDGHVTG 120
Query: 121 SLGSDSELGKIGSSSQSPTLGLNCTLPEFATERKQVDAISDPNGSMNGSSPMSSPSEIVE 180
SLG SEL KIGSSSQSP LGLNCTLPEF ER+ +DA+SDPNGSMNG+S MS PSE VE
Sbjct: 121 SLG--SELDKIGSSSQSPILGLNCTLPEFNAEREPIDALSDPNGSMNGNSSMSPPSETVE 180
Query: 181 DGVLLNGKSSDKCSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFSNNGIKINGFAEY 240
DGVLLNGK D CSSDNEMDDLNKEVVL PDY+VYGDFYCASP LTFS N IKINGFA+Y
Sbjct: 181 DGVLLNGKYLDNCSSDNEMDDLNKEVVLYPDYIVYGDFYCASPSLTFSPNAIKINGFADY 240
Query: 241 GSDEFLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACDTSGQWTSASIKE 300
GS+EFLNLEWRVDDLIDIECQ FQRVEYVMIKLHVISKDAGQCDN+CDTSG IKE
Sbjct: 241 GSNEFLNLEWRVDDLIDIECQCFQRVEYVMIKLHVISKDAGQCDNSCDTSG------IKE 300
Query: 301 VKIALVESHWSEKQQKIRSLDSRYMAIWNMSLFAGIGTDDDDLGGQRHYFPYFDEHFEEV 360
VKI LV+S+WSEKQQKIRSLDSRYMAIWNMSL AGIGTDDDDLGGQRHYFP FDE FEEV
Sbjct: 301 VKIVLVDSYWSEKQQKIRSLDSRYMAIWNMSLDAGIGTDDDDLGGQRHYFPNFDEPFEEV 360
Query: 361 VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKHRFHFFNSFFFRK 420
VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLK QIDP++KHRFHFFNSFFFRK
Sbjct: 361 VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPQDKHRFHFFNSFFFRK 420
Query: 421 LADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHPGEVARY 480
LADLDKDPSSASDGRAAFLRVRKWTRKVDLF+KDYIFIPINF+LHWSLMVICHPGEVARY
Sbjct: 421 LADLDKDPSSASDGRAAFLRVRKWTRKVDLFEKDYIFIPINFNLHWSLMVICHPGEVARY 480
Query: 481 SDEDLMKSMKVPCILHMDSIKGSHAAGLATLLAMEHQLYSGLTLGLPTLLWPLGIPTYTV 540
SDEDL KSMKVPCILHMDSIKGSHA GL L+
Sbjct: 481 SDEDLTKSMKVPCILHMDSIKGSHA-------------------GLKNLI---------- 540
Query: 541 FHKAKRRQLKRFGIFKDEEAIKWADLSLDVAPIVLSHEEDSWGWNIHPSRQFSTKSLLSD 600
Sbjct: 541 ------------------------------------------------------------ 600
Query: 601 MSDSRCLLESSLTKGIWKDNYPKKIKFFLWEISHRAISTKDVLQKRMPYLAISPDENSVP 660
Sbjct: 601 ------------------------------------------------------------ 660
Query: 661 SYKRSYLLEEWKERNKETSEDISSKFKNLRFLPLE------------------------- 720
+SYLLEEWKERNKET EDIS+KFKNLRFLPLE
Sbjct: 661 ---QSYLLEEWKERNKETPEDISTKFKNLRFLPLELPQQENSFDCGLFLLHYLELFLAEA 720
Query: 721 ---------------LNVDWFPPAEAYLKRTLIQRLIFEILENRSREMSTAACSDELLSK 780
LNVDWFPPAEAYLKRTLIQRLIFEILENRSREMSTAACSDELLSK
Sbjct: 721 PLNFSPFKIFKLSKFLNVDWFPPAEAYLKRTLIQRLIFEILENRSREMSTAACSDELLSK 780
Query: 781 FPSNNEDETGVELLPERGSPAVTCNHNLSSSQAVDGIEITLLSESSSRHNHFLDGSGLVV 840
FPSNNEDETGVELL ERGSPAV CNHNLSSSQAVDGIEITLLSESSSR NHF+DGSGLVV
Sbjct: 781 FPSNNEDETGVELLLERGSPAVACNHNLSSSQAVDGIEITLLSESSSRPNHFIDGSGLVV 840
Query: 841 RELFEPGASNGSLLGHYQSFAQTSSYFDANGTVLEEDADAETEDRFMYLPPEQDGLQPTD 900
RELFEPGASNGSLLG YQSFAQTSSYFD N TVLE+DADAET DRFMYLPP QDGLQP D
Sbjct: 841 RELFEPGASNGSLLGDYQSFAQTSSYFDTNATVLEDDADAETGDRFMYLPPVQDGLQPID 900
Query: 901 AMTSQACRFPCSSRGLESEAAFDLCMSIQPEHGGGSASSPSSHADHLADVGIIESCDVRE 960
AM SQACRFPCSS GLES+A+FDLCMSIQPE G G A SS A+ L DVGIIESC VRE
Sbjct: 901 AMASQACRFPCSSIGLESKASFDLCMSIQPERGRGIA---SSSAEDLEDVGIIESCKVRE 922
Query: 961 ASPGNKEEMSRKRPLS--TENLEQPVAECPTSPAATMLDVDAIVISQDANAICEDMDNNG 1020
ASPGNKEE +RKRPLS ENLE PVAE PTS A T+ DVD IVISQD N M +N
Sbjct: 961 ASPGNKEERNRKRPLSHEKENLE-PVAERPTSAATTVQDVDTIVISQDTN-----MGSND 922
Query: 1021 SDPP-CKETLVASPRLDEDITTKADIKHDDALVVASVVEVDLDEQPAAKKPRHSLYLEEP 1051
S+PP C++TL+ASP LDE TK D++HDD+ VV S+ EV+LDE+P AKKPRHS Y EE
Sbjct: 1021 SEPPLCEDTLIASPCLDE--ATKTDVEHDDSPVVVSITEVNLDEEPPAKKPRHSPYPEET 922
BLAST of Spg034115 vs. NCBI nr
Match:
XP_022962128.1 (probable ubiquitin-like-specific protease 2B isoform X2 [Cucurbita moschata])
HSP 1 Score: 1395.6 bits (3611), Expect = 0.0e+00
Identity = 761/1094 (69.56%), Postives = 808/1094 (73.86%), Query Frame = 0
Query: 1 MKNAAVKGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLECGKEIENPHMD 60
MKNA+VKGLEVFDFTEEDELPELISEKRLSKFKNPN++TN VLKYEFLECGKEI+NPHMD
Sbjct: 1 MKNASVKGLEVFDFTEEDELPELISEKRLSKFKNPNLDTNAVLKYEFLECGKEIKNPHMD 60
Query: 61 VDLDECNRGCDDGISHIPSGSTKEQFIMKEEKYQLDANTESELNSHSQDVFMQVDNHVTG 120
VDL +CNRGCD+GISHIPSG+TKEQFIM+E KYQLDANTESELNSHSQD+F+QVD HVTG
Sbjct: 61 VDLGKCNRGCDNGISHIPSGTTKEQFIMEEGKYQLDANTESELNSHSQDLFVQVDGHVTG 120
Query: 121 SLGSDSELGKIGSSSQSPTLGLNCTLPEFATERKQVDAISDPNGSMNGSSPMSSPSEIVE 180
SLG SEL KIGSSSQSP LGLNCTLPEF ER+ +DA+SDPNGSMNG+S MS PSE VE
Sbjct: 121 SLG--SELDKIGSSSQSPILGLNCTLPEFNAEREPIDALSDPNGSMNGNSSMSPPSETVE 180
Query: 181 DGVLLNGKSSDKCSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFSNNGIKINGFAEY 240
DGVLLNGK D CSSDNEMDDLNKEVVL PDY+VYGDFYCASP LTFS N IKINGFA+Y
Sbjct: 181 DGVLLNGKYLDNCSSDNEMDDLNKEVVLYPDYIVYGDFYCASPSLTFSPNAIKINGFADY 240
Query: 241 GSDEFLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACDTSGQWTSASIKE 300
GS+EFLNLEWRVDDLIDIECQ FQRVEYVMIKLHVISKDAGQCDN+CDTSG IKE
Sbjct: 241 GSNEFLNLEWRVDDLIDIECQCFQRVEYVMIKLHVISKDAGQCDNSCDTSG------IKE 300
Query: 301 VKIALVESHWSEKQQKIRSLDSRYMAIWNMSLFAGIGTDDDDLGGQRHYFPYFDEHFEEV 360
VKI LV+S+WSEKQQKIRSLDSRYMAIWNMSL AGIGTDDDDLGGQRHYFP FDE FEEV
Sbjct: 301 VKIVLVDSYWSEKQQKIRSLDSRYMAIWNMSLDAGIGTDDDDLGGQRHYFPNFDEPFEEV 360
Query: 361 VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKHRFHFFNSFFFRK 420
VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLK QIDP++KHRFHFFNSFFFRK
Sbjct: 361 VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPQDKHRFHFFNSFFFRK 420
Query: 421 LADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHPGEVARY 480
LADLDKDPSSASDGRAAFLRVRKWTRKVDLF+KDYIFIPINF+LHWSLMVICH GEVARY
Sbjct: 421 LADLDKDPSSASDGRAAFLRVRKWTRKVDLFEKDYIFIPINFNLHWSLMVICHLGEVARY 480
Query: 481 SDEDLMKSMKVPCILHMDSIKGSHAAGLATLLAMEHQLYSGLTLGLPTLLWPLGIPTYTV 540
SDEDL KSMKVPCILHMDSIKGSHA GL L+
Sbjct: 481 SDEDLTKSMKVPCILHMDSIKGSHA-------------------GLKNLI---------- 540
Query: 541 FHKAKRRQLKRFGIFKDEEAIKWADLSLDVAPIVLSHEEDSWGWNIHPSRQFSTKSLLSD 600
Sbjct: 541 ------------------------------------------------------------ 600
Query: 601 MSDSRCLLESSLTKGIWKDNYPKKIKFFLWEISHRAISTKDVLQKRMPYLAISPDENSVP 660
Sbjct: 601 ------------------------------------------------------------ 660
Query: 661 SYKRSYLLEEWKERNKETSEDISSKFKNLRFLPLE------------------------- 720
+SYLLEEWKERNKET EDIS+KFKNLRFLPLE
Sbjct: 661 ---QSYLLEEWKERNKETPEDISTKFKNLRFLPLELPQQENSFDCGLFLLHYLELFLAEA 720
Query: 721 ---------------LNVDWFPPAEAYLKRTLIQRLIFEILENRSREMSTAACSDELLSK 780
LNVDWFPPAEAYLKRTLIQRLIFEILENRSREMSTAACSDELLSK
Sbjct: 721 PLSFSPFKIFKLSKFLNVDWFPPAEAYLKRTLIQRLIFEILENRSREMSTAACSDELLSK 780
Query: 781 FPSNNEDETGVELLPERGSPAVTCNHNLSSSQAVDGIEITLLSESSSRHNHFLDGSGLVV 840
FPSNNEDETGVELL ERGSPAV CNHNLSSSQAVDGIEITLLSESSSR NHF+DGSGLVV
Sbjct: 781 FPSNNEDETGVELLLERGSPAVACNHNLSSSQAVDGIEITLLSESSSRPNHFIDGSGLVV 840
Query: 841 RELFEPGASNGSLLGHYQSFAQTSSYFDANGTVLEEDADAETEDRFMYLPPEQDGLQPTD 900
RELFEPGASNGSLLG YQSFAQTSSYFD N TVLE+DADAET DRFM+LPP QDGLQP D
Sbjct: 841 RELFEPGASNGSLLGDYQSFAQTSSYFDTNATVLEDDADAETGDRFMFLPPVQDGLQPID 900
Query: 901 AMTSQACRFPCSSRGLESEAAFDLCMSIQPEHGGGSASSPSSHADHLADVGIIESCDVRE 960
AM SQACRFPCSS GLES+A+FDLCMSIQPE G G A SS A+ L DVGIIESCDVRE
Sbjct: 901 AMASQACRFPCSSIGLESKASFDLCMSIQPERGRGIA---SSSAEDLEDVGIIESCDVRE 923
Query: 961 ASPGNKEEMSRKRPLS--TENLEQPVAECPTSPAATMLDVDAIVISQDANAICEDMDNNG 1020
ASPGNKE+ +RKRPLS ENLE PV E PTS A T+ DVD IVISQD N M +N
Sbjct: 961 ASPGNKEDRNRKRPLSHEQENLE-PVTERPTSAATTVQDVDTIVISQDTN-----MGSND 923
Query: 1021 SDPPCK-ETLVASPRLDEDITTKADIKHDDALVVASVVEVDLDEQPAAKKPRHSLYLEEP 1052
S+P C+ +TLVASP LDE TK D++HDD VV S+ EV+LDE+P AKKPRHS Y EE
Sbjct: 1021 SEPLCEDDTLVASPCLDE--ATKTDVEHDDLPVVVSITEVNLDEEPPAKKPRHSPYPEET 923
BLAST of Spg034115 vs. ExPASy Swiss-Prot
Match:
Q8L7S0 (Probable ubiquitin-like-specific protease 2B OS=Arabidopsis thaliana OX=3702 GN=ULP2B PE=1 SV=3)
HSP 1 Score: 487.3 bits (1253), Expect = 4.5e-136
Identity = 364/1114 (32.68%), Postives = 515/1114 (46.23%), Query Frame = 0
Query: 7 KGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLE--CG-KEIENPHMD--- 66
K EVFDF EEDEL E + K L KF NP+ +PVL+ + ++ C K +E M+
Sbjct: 3 KNFEVFDFKEEDELAESAAGKLLEKFTNPSPCNSPVLQRQRIQSFCNEKRVEEEEMEGPS 62
Query: 67 -----VDLDECNRGCDDGISHIPSGSTKEQFI-------------------------MKE 126
++ + C+D + + + +K
Sbjct: 63 CAEPATAVESDDHQCEDDSTLVTEAKESRTILTFGLETTDHLEETDAEHVNQGLMLGLKT 122
Query: 127 EKYQLDANTESE-------LNSHSQDVFMQVDNHVTG---SLGSDSELGKIGSSSQSPTL 186
E + + + + LNS VD+ V LG +S + S SQ
Sbjct: 123 EDLAKETDIDHDNHGLMFGLNSEDDIEETDVDHRVESFSCQLGGNSFYAETSSYSQRQ-- 182
Query: 187 GLNCTLPEFATERKQVDAISDPNGSMNGSSPMSSPSEIVEDGVLLNGKSSDKCSSDNEMD 246
LN + ++ +Q+D +S + S++ S +S S+ +D ++ C +D E
Sbjct: 183 -LNSPFSDSSSSEEQIDMMSAIDESLSDRSALSEASDSEDDE---EDWMTEHCFNDEEKI 242
Query: 247 DLNKEVVLCPDYVVYGDFYCASPLLTFSNNGIKINGFAEYGSDEFLNLEWRVDDLIDIEC 306
DL+ V++ +YV+ D +CA+ L+ FS NGIKI F + + E+ V+D++ I+
Sbjct: 243 DLSTAVIMTSEYVILKDMHCAASLVIFSCNGIKIKSFLANNEEVPFSCEFGVEDIVSIQY 302
Query: 307 QWFQRVEYVMIKLHVISKDAGQCDNACDTSGQWTSASIKEVKIALVESHWSEKQQKIRSL 366
W+Q V +++++ V+ KD ++E+KIA+ E +W KQQKI SL
Sbjct: 303 NWYQNVGLIILRIRVLLKDEN------------CHEDMEELKIAVKEHNWPNKQQKINSL 362
Query: 367 DSRYMAIWNMSLFAGIGTDDDDLGGQRHYFPYFDEHFEEVVYPKGDPDAVSISKRDVDLL 426
+Y A+WN L + +L Q+ YFP FDE FE+VVYPKGDPDAVSI KRDV+LL
Sbjct: 363 HVKYPAVWNTDLEDDVEVSGYNLNQQKRYFPSFDEPFEDVVYPKGDPDAVSICKRDVELL 422
Query: 427 QPETFVNDTIIDFYIQYLKIQIDPKEKHRFHFFNSFFFRKLADLDKDPSSASDGRAAFLR 486
QPETFVNDTIIDFYI YLK QI +EKHRFHFFNSFFFRKLADLDKDPSS +DG+AAFLR
Sbjct: 423 QPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIADGKAAFLR 482
Query: 487 VRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHPGEVARYSDEDLMKSMKVPCILHMDSI 546
VRKWTRKVD+F KDYIF+P+N++LHWSL+VICHPGEVA +D DL S KVPCILHMDSI
Sbjct: 483 VRKWTRKVDMFGKDYIFVPVNYNLHWSLIVICHPGEVANRTDLDLDDSKKVPCILHMDSI 542
Query: 547 KGSHAAGLATLLAMEHQLYSGLTLGLPTLLWPLGIPTYTVFHKAKRRQLKRFGIFKDEEA 606
KGSHA GL L+
Sbjct: 543 KGSHA-------------------GLKNLV------------------------------ 602
Query: 607 IKWADLSLDVAPIVLSHEEDSWGWNIHPSRQFSTKSLLSDMSDSRCLLESSLTKGIWKDN 666
Sbjct: 603 ------------------------------------------------------------ 662
Query: 667 YPKKIKFFLWEISHRAISTKDVLQKRMPYLAISPDENSVPSYKRSYLLEEWKERNKETSE 726
++YL EEWKER+KETS+
Sbjct: 663 -------------------------------------------QTYLCEEWKERHKETSD 722
Query: 727 DISSKFKNLRFLPLE----------------------------------------LNVDW 786
DISS+F NLRF+ LE L ++W
Sbjct: 723 DISSRFMNLRFVSLELPQQENSFDCGLFLLHYLELFLAEAPLNFSPFKIYNASNFLYLNW 782
Query: 787 FPPAEAYLKRTLIQRLIFEILENRSREMSTAACSDELLSKFPSNNEDETGVELLPERGSP 846
FPPAEA LKRTLIQ+LIFE+LENRSRE+S + P D+ G+E+L ER SP
Sbjct: 783 FPPAEASLKRTLIQKLIFELLENRSREVSN---EQNQSCESPVAVNDDMGIEVLSERCSP 842
Query: 847 AVTCNHNLSSSQAVDGIEITLLSESSSRHNHFLDGSGLVVRELFEPGASN-GSLLGHYQS 906
+ CN +++ +Q GIE+TLL SS RH + SG+V+R+LF+ G++N GSLL Q
Sbjct: 843 LIDCNGDMTQTQDDQGIEMTLLERSSMRHIQAANDSGMVLRDLFDSGSNNTGSLLEQLQQ 902
Query: 907 -FAQTSSYFD-ANGTVLEEDADAETEDRFMYLPPEQDGLQPTDAMTSQACRFPCSSRGLE 966
F SS++ +N + E D ET ++FM L + Q T S R
Sbjct: 903 PFEDPSSFYHLSNDSSAREQVDMETGEQFMCLNAGEGNFQCITETT--------SPRASN 920
Query: 967 SEAAFDLCMSIQPEHGGGSASSPSSHADHLADVGIIESCDVREASPGNKEEMSRKRPLST 1026
S ++++L + + + S +S++ + +GIIE + ++EE+ P T
Sbjct: 963 SFSSWNLGIPLVQKEDETDLLSETSNSSN-DSIGIIEDNPIENT---HEEEIDESPPRET 920
Query: 1027 ENLEQPVAECPTSPAATMLDVDAIVISQDANAICEDMDNNGSDPPCKETLVASPRLDEDI 1032
+L+ T+ T + + V + + ++ + P + L + ED+
Sbjct: 1023 VSLKSATVGSNTADHTT--ENEEPVSTHIELVVPSSQNDRDEEKPLEHDLEIGDKTSEDV 920
BLAST of Spg034115 vs. ExPASy Swiss-Prot
Match:
Q0WKV8 (Probable ubiquitin-like-specific protease 2A OS=Arabidopsis thaliana OX=3702 GN=ULP2A PE=2 SV=2)
HSP 1 Score: 299.7 bits (766), Expect = 1.3e-79
Identity = 185/509 (36.35%), Postives = 272/509 (53.44%), Query Frame = 0
Query: 7 KGLEVFDFTEEDELPELISEKRLSKFKNPNIETN--PVLKYEFLECGKEIENPHMDVDLD 66
K ++VFD+++ED+ E S+K L KF +P + + + KYEFL C
Sbjct: 13 KPIDVFDYSDEDDRVEEESKKLLRKFDSPVTKKHHCAIDKYEFLRC-------------- 72
Query: 67 ECNRGCDDGISHIPSGSTKEQFIMKEEKYQLDANTESELNSHSQDVFMQVDNHVTGSLGS 126
+ D +ES++ H + + V+ V S
Sbjct: 73 ----------------------------FAKDTQSESKVLQH---IVIDVEVPVKEE-PS 132
Query: 127 DSELGKIGSSSQSPTLGLNCTLPEFATERKQVDAISDPNGSMNGSSPMSSPSEIVEDGVL 186
EL G+S +D IS+ + G ++S S + E+ +
Sbjct: 133 RCELSGDGNSD-------------------LIDVISNGSHRRIGIDSLTS-SSLSENDEV 192
Query: 187 LNGKSSDKCSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFSNNGIKINGFAEYGSDE 246
G++++ S +E+D N +V++ PD ++YGD YC + LTFS N + + + +
Sbjct: 193 STGEATNPASDPHEVDPENAQVLIIPDVIIYGDIYCTNSKLTFSRNCMNVESSSVNATKG 252
Query: 247 FLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACDTSGQWTSASIKEVKIA 306
+ +W ++D+I IE QW VE + + + S+ D A D SG I +K +
Sbjct: 253 TFSCQWTIEDIIKIESQWCLEVETAFVNVLLKSRKPEGVDIAKDISG------IDLLKFS 312
Query: 307 LVESHWSEKQQKIRSLDSRYMAIW--NMSLFAGIGTDDDDLGGQRHYFPYFDEHFEEVVY 366
+ + WS++ + IRSLDSRY IW ++ I DLG + FE++VY
Sbjct: 313 VYDPKWSKEVETIRSLDSRYKNIWFDTITESEEIAFSGHDLGTS---LTNLADSFEDLVY 372
Query: 367 PKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKHRFHFFNSFFFRKLA 426
P+G+PDAV + K+D++LL+P F+NDTIIDFYI+YLK +I PKE+ RFHFFN FFFRKLA
Sbjct: 373 PQGEPDAVVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKERGRFHFFNCFFFRKLA 432
Query: 427 DLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHPGEVARYSD 486
+LDK S GR A+ RV+KWT+ VDLF+KDYIFIPIN S HWSL++ICHPGE+
Sbjct: 433 NLDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCSFHWSLVIICHPGELVPSHV 443
Query: 487 EDLMKSMKVPCILHMDSIKGSHAAGLATL 512
E+ +VPCILH+DSIKGSH GL +
Sbjct: 493 EN---PQRVPCILHLDSIKGSHKGGLINI 443
BLAST of Spg034115 vs. ExPASy Swiss-Prot
Match:
O13769 (Ubiquitin-like-specific protease 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ulp2 PE=1 SV=2)
HSP 1 Score: 100.5 bits (249), Expect = 1.2e-19
Identity = 55/142 (38.73%), Postives = 81/142 (57.04%), Query Frame = 0
Query: 360 VVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYL--KIQI-DPKEKHRFHFFNSF 419
+VYP ++++I+ D+ L F+NDTI+DFY++YL K+Q +P + H FN+F
Sbjct: 337 LVYPFSGTNSIAITNTDLTRLNEGEFLNDTIVDFYLRYLYCKLQTQNPSLANDTHIFNTF 396
Query: 420 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHPGE 479
F+ +L DKD R VRKWT+KVDLF K YI +PIN + HW L +IC+
Sbjct: 397 FYNRLTSKDKDGK-----RLGHRGVRKWTQKVDLFHKKYIIVPINETFHWYLAIICNIDR 456
Query: 480 V------ARYSDEDLMKSMKVP 493
+ DE +M S++ P
Sbjct: 457 LMPVDTKLEEQDEIVMSSVEQP 473
BLAST of Spg034115 vs. ExPASy Swiss-Prot
Match:
Q6P7W0 (Sentrin-specific protease 6 OS=Mus musculus OX=10090 GN=Senp6 PE=1 SV=3)
HSP 1 Score: 99.4 bits (246), Expect = 2.6e-19
Identity = 50/142 (35.21%), Postives = 85/142 (59.86%), Query Frame = 0
Query: 339 DDDDLGGQRHYFPYFDEHFEEVVYPKGD-PDAVSISKRDVDLLQPETFVNDTIIDFYIQY 398
DDD+ G+ H + + +VYP +S++ D+ L F+ND IIDFY++Y
Sbjct: 655 DDDEEAGESHTI-FIGPVEKLIVYPPPPAKGGISVTNEDLHCLSEGEFLNDVIIDFYLKY 714
Query: 399 LKIQ-IDPKEKHRFHFFNSFFFRKLADLDK---DPSSASDGRAAFLRVRKWTRKVDLFDK 458
L ++ + ++ R H F+SFF+++L ++ + ++ S + RV+ WTR VD+F+K
Sbjct: 715 LVLEKLKKEDADRIHIFSSFFYKRLNQRERRNPETTNLSIQQKRHGRVKTWTRHVDIFEK 774
Query: 459 DYIFIPINFSLHWSLMVICHPG 476
D+IF+P+N + HW L V+C PG
Sbjct: 775 DFIFVPLNEAAHWFLAVVCFPG 795
BLAST of Spg034115 vs. ExPASy Swiss-Prot
Match:
Q2PS26 (Ubiquitin-like-specific protease 1D OS=Arabidopsis thaliana OX=3702 GN=ULP1D PE=1 SV=1)
HSP 1 Score: 98.6 bits (244), Expect = 4.5e-19
Identity = 54/151 (35.76%), Postives = 90/151 (59.60%), Query Frame = 0
Query: 358 EEVVYP-KGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEK--HRFHFFN 417
E++ YP + DP V + +D++ L P ++ +++FY+++L+ QI + HFFN
Sbjct: 330 EDICYPTRDDPHFVQVCLKDLECLAPREYLTSPVMNFYMRFLQQQISSSNQISADCHFFN 389
Query: 418 SFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHP 477
++F++KL+ D +D A F+R R+W + +DLF K YIFIPI+ LHWSL+++C P
Sbjct: 390 TYFYKKLS--DAVTYKGNDKDAFFVRFRRWWKGIDLFRKAYIFIPIHEDLHWSLVIVCIP 449
Query: 478 GEVARYSDEDLMKSMKVPCILHMDSIKGSHA 506
+ DE + ILH+DS+ G H+
Sbjct: 450 DK----KDESGL------TILHLDSL-GLHS 467
BLAST of Spg034115 vs. ExPASy TrEMBL
Match:
A0A6J1HG30 (probable ubiquitin-like-specific protease 2B isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462674 PE=3 SV=1)
HSP 1 Score: 1406.3 bits (3639), Expect = 0.0e+00
Identity = 761/1066 (71.39%), Postives = 808/1066 (75.80%), Query Frame = 0
Query: 1 MKNAAVKGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLEC---------- 60
MKNA+VKGLEVFDFTEEDELPELISEKRLSKFKNPN++TN VLKYEFLEC
Sbjct: 1 MKNASVKGLEVFDFTEEDELPELISEKRLSKFKNPNLDTNAVLKYEFLECDKLTVANGFV 60
Query: 61 --GKEIENPHMDVDLDECNRGCDDGISHIPSGSTKEQFIMKEEKYQLDANTESELNSHSQ 120
GKEI+NPHMDVDL +CNRGCD+GISHIPSG+TKEQFIM+E KYQLDANTESELNSHSQ
Sbjct: 61 VEGKEIKNPHMDVDLGKCNRGCDNGISHIPSGTTKEQFIMEEGKYQLDANTESELNSHSQ 120
Query: 121 DVFMQVDNHVTGSLGSDSELGKIGSSSQSPTLGLNCTLPEFATERKQVDAISDPNGSMNG 180
D+F+QVD HVTGSLG SEL KIGSSSQSP LGLNCTLPEF ER+ +DA+SDPNGSMNG
Sbjct: 121 DLFVQVDGHVTGSLG--SELDKIGSSSQSPILGLNCTLPEFNAEREPIDALSDPNGSMNG 180
Query: 181 SSPMSSPSEIVEDGVLLNGKSSDKCSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFS 240
+S MS PSE VEDGVLLNGK D CSSDNEMDDLNKEVVL PDY+VYGDFYCASP LTFS
Sbjct: 181 NSSMSPPSETVEDGVLLNGKYLDNCSSDNEMDDLNKEVVLYPDYIVYGDFYCASPSLTFS 240
Query: 241 NNGIKINGFAEYGSDEFLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACD 300
N IKINGFA+YGS+EFLNLEWRVDDLIDIECQ FQRVEYVMIKLHVISKDAGQCDN+CD
Sbjct: 241 PNAIKINGFADYGSNEFLNLEWRVDDLIDIECQCFQRVEYVMIKLHVISKDAGQCDNSCD 300
Query: 301 TSGQWTSASIKEVKIALVESHWSEKQQKIRSLDSRYMAIWNMSLFAGIGTDDDDLGGQRH 360
TSG IKEVKI LV+S+WSEKQQKIRSLDSRYMAIWNMSL AGIGTDDDDLGGQRH
Sbjct: 301 TSG------IKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNMSLDAGIGTDDDDLGGQRH 360
Query: 361 YFPYFDEHFEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKH 420
YFP FDE FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLK QIDP++KH
Sbjct: 361 YFPNFDEPFEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPQDKH 420
Query: 421 RFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSL 480
RFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLF+KDYIFIPINF+LHWSL
Sbjct: 421 RFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFEKDYIFIPINFNLHWSL 480
Query: 481 MVICHPGEVARYSDEDLMKSMKVPCILHMDSIKGSHAAGLATLLAMEHQLYSGLTLGLPT 540
MVICH GEVARYSDEDL KSMKVPCILHMDSIKGSHA GL
Sbjct: 481 MVICHLGEVARYSDEDLTKSMKVPCILHMDSIKGSHA-------------------GLKN 540
Query: 541 LLWPLGIPTYTVFHKAKRRQLKRFGIFKDEEAIKWADLSLDVAPIVLSHEEDSWGWNIHP 600
L+
Sbjct: 541 LI---------------------------------------------------------- 600
Query: 601 SRQFSTKSLLSDMSDSRCLLESSLTKGIWKDNYPKKIKFFLWEISHRAISTKDVLQKRMP 660
Sbjct: 601 ------------------------------------------------------------ 660
Query: 661 YLAISPDENSVPSYKRSYLLEEWKERNKETSEDISSKFKNLRFLPLELNVDWFPPAEAYL 720
+SYLLEEWKERNKET EDIS+KFKNLRFLPLELNVDWFPPAEAYL
Sbjct: 661 ---------------QSYLLEEWKERNKETPEDISTKFKNLRFLPLELNVDWFPPAEAYL 720
Query: 721 KRTLIQRLIFEILENRSREMSTAACSDELLSKFPSNNEDETGVELLPERGSPAVTCNHNL 780
KRTLIQRLIFEILENRSREMSTAACSDELLSKFPSNNEDETGVELL ERGSPAV CNHNL
Sbjct: 721 KRTLIQRLIFEILENRSREMSTAACSDELLSKFPSNNEDETGVELLLERGSPAVACNHNL 780
Query: 781 SSSQAVDGIEITLLSESSSRHNHFLDGSGLVVRELFEPGASNGSLLGHYQSFAQTSSYFD 840
SSSQAVDGIEITLLSESSSR NHF+DGSGLVVRELFEPGASNGSLLG YQSFAQTSSYFD
Sbjct: 781 SSSQAVDGIEITLLSESSSRPNHFIDGSGLVVRELFEPGASNGSLLGDYQSFAQTSSYFD 840
Query: 841 ANGTVLEEDADAETEDRFMYLPPEQDGLQPTDAMTSQACRFPCSSRGLESEAAFDLCMSI 900
N TVLE+DADAET DRFM+LPP QDGLQP DAM SQACRFPCSS GLES+A+FDLCMSI
Sbjct: 841 TNATVLEDDADAETGDRFMFLPPVQDGLQPIDAMASQACRFPCSSIGLESKASFDLCMSI 895
Query: 901 QPEHGGGSASSPSSHADHLADVGIIESCDVREASPGNKEEMSRKRPLS--TENLEQPVAE 960
QPE G G A SS A+ L DVGIIESCDVREASPGNKE+ +RKRPLS ENLE PV E
Sbjct: 901 QPERGRGIA---SSSAEDLEDVGIIESCDVREASPGNKEDRNRKRPLSHEQENLE-PVTE 895
Query: 961 CPTSPAATMLDVDAIVISQDANAICEDMDNNGSDPPCK-ETLVASPRLDEDITTKADIKH 1020
PTS A T+ DVD IVISQD N M +N S+P C+ +TLVASP LDE TK D++H
Sbjct: 961 RPTSAATTVQDVDTIVISQDTN-----MGSNDSEPLCEDDTLVASPCLDE--ATKTDVEH 895
Query: 1021 DDALVVASVVEVDLDEQPAAKKPRHSLYLEEPSNGLKEGLSDDADL 1052
DD VV S+ EV+LDE+P AKKPRHS Y EE SNG+ EGLSD AD+
Sbjct: 1021 DDLPVVVSITEVNLDEEPPAKKPRHSPYPEETSNGVGEGLSDGADI 895
BLAST of Spg034115 vs. ExPASy TrEMBL
Match:
A0A6J1GIE5 (probable ubiquitin-like-specific protease 2B isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454203 PE=3 SV=1)
HSP 1 Score: 1400.2 bits (3623), Expect = 0.0e+00
Identity = 739/1051 (70.31%), Postives = 793/1051 (75.45%), Query Frame = 0
Query: 1 MKNAAVKGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLECGKEIENPHMD 60
M +A+VKGLEVFDFTEEDELPELISEK LSKFKNPN+ETN VLKY LE GKE+ENPHMD
Sbjct: 1 MNDASVKGLEVFDFTEEDELPELISEKCLSKFKNPNLETNSVLKYGCLELGKEVENPHMD 60
Query: 61 VDLDECNRGCDDGISHIPSGSTKEQFIMKEEKYQLDANTESELNSHSQDVFMQVDNHVTG 120
VDLDECNRGCD+GISHIP G +KEQ IM+EEKYQLD NTESE N+HSQD+F+QV+NHVTG
Sbjct: 61 VDLDECNRGCDNGISHIPPGISKEQLIMEEEKYQLDVNTESEWNTHSQDMFVQVNNHVTG 120
Query: 121 SLGSDSELGKIGSSSQSPTLGLNCTLPEFATERKQVDAISDPNGSMNGSSPMSSPSEIVE 180
G SELGK+GSSSQS LGLNC+LPEFA +R+QVDA+S PNGSMNGSSPMSSPSE+VE
Sbjct: 121 CFG--SELGKVGSSSQSSILGLNCSLPEFAAKREQVDALSYPNGSMNGSSPMSSPSELVE 180
Query: 181 DGVLLNGKSSDKCSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFSNNGIKINGFAEY 240
D V NGKSSDKCSSDNEMDDLN+EVVLCPDY++YGD YC S LTFS+NG+KINGF++Y
Sbjct: 181 DSVSPNGKSSDKCSSDNEMDDLNQEVVLCPDYIIYGDSYCTSSQLTFSHNGVKINGFSDY 240
Query: 241 GSDEFLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACDTSGQWTSASIKE 300
GS+EFLNLEW VDDLI+IEC WFQRVE+V IKLH ISKD GQ DN CDTSG +KE
Sbjct: 241 GSNEFLNLEWGVDDLINIECHWFQRVEFVTIKLHTISKDVGQRDNGCDTSG------LKE 300
Query: 301 VKIALVESHWSEKQQKIRSLDSRYMAIWNMSLFAGIGTDDDDLGGQRHYFPYFDEHFEEV 360
VKI LV+ WSEKQQKIRSLDSRYMAIWNMSL G DDDLGG R YFP FDEHFEEV
Sbjct: 301 VKIVLVDPCWSEKQQKIRSLDSRYMAIWNMSLDVG----DDDLGGPRQYFPNFDEHFEEV 360
Query: 361 VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKHRFHFFNSFFFRK 420
VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLK QIDPKEKHRFHFFNSFFFRK
Sbjct: 361 VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPKEKHRFHFFNSFFFRK 420
Query: 421 LADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHPGEVARY 480
LADLDKDPSSASDG+AAFLRVRKWTRKV+LFDKDYIFIPINF+LHWSLMVICHPGEVARY
Sbjct: 421 LADLDKDPSSASDGKAAFLRVRKWTRKVNLFDKDYIFIPINFNLHWSLMVICHPGEVARY 480
Query: 481 SDEDLMKSMKVPCILHMDSIKGSHAAGLATLLAMEHQLYSGLTLGLPTLLWPLGIPTYTV 540
SDE+LMKS KVPCILHMDSIKGSHA GL L+
Sbjct: 481 SDENLMKSTKVPCILHMDSIKGSHA-------------------GLKNLI---------- 540
Query: 541 FHKAKRRQLKRFGIFKDEEAIKWADLSLDVAPIVLSHEEDSWGWNIHPSRQFSTKSLLSD 600
Sbjct: 541 ------------------------------------------------------------ 600
Query: 601 MSDSRCLLESSLTKGIWKDNYPKKIKFFLWEISHRAISTKDVLQKRMPYLAISPDENSVP 660
Sbjct: 601 ------------------------------------------------------------ 660
Query: 661 SYKRSYLLEEWKERNKETSEDISSKFKNLRFLPLELNVDWFPPAEAYLKRTLIQRLIFEI 720
+SYLLEEW ERNK+ SEDISSKFKNLRFLPLELNVDWFPPAEAYLKRTLIQRLIFEI
Sbjct: 661 ---QSYLLEEWNERNKDASEDISSKFKNLRFLPLELNVDWFPPAEAYLKRTLIQRLIFEI 720
Query: 721 LENRSREMSTAACSDELLSKFPSNNEDETGVELLPERGSPAVTCNHNLSSSQAVDGIEIT 780
LE RSR+MST ACSD+LLSKFPSNNEDE GVEL+PE G A T NHNLSSSQA DGIEIT
Sbjct: 721 LEKRSRQMSTTACSDDLLSKFPSNNEDEAGVELVPESGRLAETFNHNLSSSQAADGIEIT 780
Query: 781 LLSESSSRHNHFLDGSGLVVRELFEPGASNGSLLGHYQSFAQTSSYFDANGTVLEEDADA 840
LLSESSSRHNHF+DGSGLVVRELFEPG SNGSLLGHYQSFAQTSSYFD N TVLEEDAD
Sbjct: 781 LLSESSSRHNHFMDGSGLVVRELFEPGTSNGSLLGHYQSFAQTSSYFDTNVTVLEEDADT 840
Query: 841 ETEDRFMYLPPEQDGLQPTDAMTSQACRFPCSSRGLESEAAFDLCMSIQPEHGGGSASSP 900
ET DRFMYLP EQDGLQP DAMTSQACRFP SSRGLESE AFDLCMSIQPEHG G ASSP
Sbjct: 841 ETGDRFMYLPSEQDGLQPVDAMTSQACRFPGSSRGLESETAFDLCMSIQPEHGSGIASSP 886
Query: 901 SSHADHLADVGIIESCDVREASPGNKEEMSRKRPLSTENLEQPVAECPTSPAATMLDVDA 960
SSH+D L DVGIIESCDVRE SPGNKEE++RKRPL ENLE P+AECPTS A T D D
Sbjct: 901 SSHSDVLEDVGIIESCDVREPSPGNKEEINRKRPLPIENLE-PIAECPTSAATTTQDTDT 886
Query: 961 IVISQDANAICEDMDNNGSDPPCKETLVASPRLDEDITTKADIKHDDALVVASVVEVDLD 1020
IV+S+D N CEDM+N+GSDP CKET+VASP LDEDITTK DI+HDDA++VASV EVDL
Sbjct: 961 IVVSKDTNDTCEDMENDGSDPHCKETVVASPCLDEDITTKTDIEHDDAVLVASVTEVDLH 886
Query: 1021 EQPAAKKPRHSLYLEEPSNGLKEGLSDDADL 1052
EQP AKK RH Y EE SNGL + L +DADL
Sbjct: 1021 EQPPAKKSRHLPYPEEASNGLGDVLLEDADL 886
BLAST of Spg034115 vs. ExPASy TrEMBL
Match:
A0A6J1HDX0 (probable ubiquitin-like-specific protease 2B isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462674 PE=3 SV=1)
HSP 1 Score: 1395.6 bits (3611), Expect = 0.0e+00
Identity = 761/1094 (69.56%), Postives = 808/1094 (73.86%), Query Frame = 0
Query: 1 MKNAAVKGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLECGKEIENPHMD 60
MKNA+VKGLEVFDFTEEDELPELISEKRLSKFKNPN++TN VLKYEFLECGKEI+NPHMD
Sbjct: 1 MKNASVKGLEVFDFTEEDELPELISEKRLSKFKNPNLDTNAVLKYEFLECGKEIKNPHMD 60
Query: 61 VDLDECNRGCDDGISHIPSGSTKEQFIMKEEKYQLDANTESELNSHSQDVFMQVDNHVTG 120
VDL +CNRGCD+GISHIPSG+TKEQFIM+E KYQLDANTESELNSHSQD+F+QVD HVTG
Sbjct: 61 VDLGKCNRGCDNGISHIPSGTTKEQFIMEEGKYQLDANTESELNSHSQDLFVQVDGHVTG 120
Query: 121 SLGSDSELGKIGSSSQSPTLGLNCTLPEFATERKQVDAISDPNGSMNGSSPMSSPSEIVE 180
SLG SEL KIGSSSQSP LGLNCTLPEF ER+ +DA+SDPNGSMNG+S MS PSE VE
Sbjct: 121 SLG--SELDKIGSSSQSPILGLNCTLPEFNAEREPIDALSDPNGSMNGNSSMSPPSETVE 180
Query: 181 DGVLLNGKSSDKCSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFSNNGIKINGFAEY 240
DGVLLNGK D CSSDNEMDDLNKEVVL PDY+VYGDFYCASP LTFS N IKINGFA+Y
Sbjct: 181 DGVLLNGKYLDNCSSDNEMDDLNKEVVLYPDYIVYGDFYCASPSLTFSPNAIKINGFADY 240
Query: 241 GSDEFLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACDTSGQWTSASIKE 300
GS+EFLNLEWRVDDLIDIECQ FQRVEYVMIKLHVISKDAGQCDN+CDTSG IKE
Sbjct: 241 GSNEFLNLEWRVDDLIDIECQCFQRVEYVMIKLHVISKDAGQCDNSCDTSG------IKE 300
Query: 301 VKIALVESHWSEKQQKIRSLDSRYMAIWNMSLFAGIGTDDDDLGGQRHYFPYFDEHFEEV 360
VKI LV+S+WSEKQQKIRSLDSRYMAIWNMSL AGIGTDDDDLGGQRHYFP FDE FEEV
Sbjct: 301 VKIVLVDSYWSEKQQKIRSLDSRYMAIWNMSLDAGIGTDDDDLGGQRHYFPNFDEPFEEV 360
Query: 361 VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKHRFHFFNSFFFRK 420
VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLK QIDP++KHRFHFFNSFFFRK
Sbjct: 361 VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPQDKHRFHFFNSFFFRK 420
Query: 421 LADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHPGEVARY 480
LADLDKDPSSASDGRAAFLRVRKWTRKVDLF+KDYIFIPINF+LHWSLMVICH GEVARY
Sbjct: 421 LADLDKDPSSASDGRAAFLRVRKWTRKVDLFEKDYIFIPINFNLHWSLMVICHLGEVARY 480
Query: 481 SDEDLMKSMKVPCILHMDSIKGSHAAGLATLLAMEHQLYSGLTLGLPTLLWPLGIPTYTV 540
SDEDL KSMKVPCILHMDSIKGSHA GL L+
Sbjct: 481 SDEDLTKSMKVPCILHMDSIKGSHA-------------------GLKNLI---------- 540
Query: 541 FHKAKRRQLKRFGIFKDEEAIKWADLSLDVAPIVLSHEEDSWGWNIHPSRQFSTKSLLSD 600
Sbjct: 541 ------------------------------------------------------------ 600
Query: 601 MSDSRCLLESSLTKGIWKDNYPKKIKFFLWEISHRAISTKDVLQKRMPYLAISPDENSVP 660
Sbjct: 601 ------------------------------------------------------------ 660
Query: 661 SYKRSYLLEEWKERNKETSEDISSKFKNLRFLPLE------------------------- 720
+SYLLEEWKERNKET EDIS+KFKNLRFLPLE
Sbjct: 661 ---QSYLLEEWKERNKETPEDISTKFKNLRFLPLELPQQENSFDCGLFLLHYLELFLAEA 720
Query: 721 ---------------LNVDWFPPAEAYLKRTLIQRLIFEILENRSREMSTAACSDELLSK 780
LNVDWFPPAEAYLKRTLIQRLIFEILENRSREMSTAACSDELLSK
Sbjct: 721 PLSFSPFKIFKLSKFLNVDWFPPAEAYLKRTLIQRLIFEILENRSREMSTAACSDELLSK 780
Query: 781 FPSNNEDETGVELLPERGSPAVTCNHNLSSSQAVDGIEITLLSESSSRHNHFLDGSGLVV 840
FPSNNEDETGVELL ERGSPAV CNHNLSSSQAVDGIEITLLSESSSR NHF+DGSGLVV
Sbjct: 781 FPSNNEDETGVELLLERGSPAVACNHNLSSSQAVDGIEITLLSESSSRPNHFIDGSGLVV 840
Query: 841 RELFEPGASNGSLLGHYQSFAQTSSYFDANGTVLEEDADAETEDRFMYLPPEQDGLQPTD 900
RELFEPGASNGSLLG YQSFAQTSSYFD N TVLE+DADAET DRFM+LPP QDGLQP D
Sbjct: 841 RELFEPGASNGSLLGDYQSFAQTSSYFDTNATVLEDDADAETGDRFMFLPPVQDGLQPID 900
Query: 901 AMTSQACRFPCSSRGLESEAAFDLCMSIQPEHGGGSASSPSSHADHLADVGIIESCDVRE 960
AM SQACRFPCSS GLES+A+FDLCMSIQPE G G A SS A+ L DVGIIESCDVRE
Sbjct: 901 AMASQACRFPCSSIGLESKASFDLCMSIQPERGRGIA---SSSAEDLEDVGIIESCDVRE 923
Query: 961 ASPGNKEEMSRKRPLS--TENLEQPVAECPTSPAATMLDVDAIVISQDANAICEDMDNNG 1020
ASPGNKE+ +RKRPLS ENLE PV E PTS A T+ DVD IVISQD N M +N
Sbjct: 961 ASPGNKEDRNRKRPLSHEQENLE-PVTERPTSAATTVQDVDTIVISQDTN-----MGSND 923
Query: 1021 SDPPCK-ETLVASPRLDEDITTKADIKHDDALVVASVVEVDLDEQPAAKKPRHSLYLEEP 1052
S+P C+ +TLVASP LDE TK D++HDD VV S+ EV+LDE+P AKKPRHS Y EE
Sbjct: 1021 SEPLCEDDTLVASPCLDE--ATKTDVEHDDLPVVVSITEVNLDEEPPAKKPRHSPYPEET 923
BLAST of Spg034115 vs. ExPASy TrEMBL
Match:
A0A6J1KRR7 (probable ubiquitin-like-specific protease 2B isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495917 PE=3 SV=1)
HSP 1 Score: 1386.7 bits (3588), Expect = 0.0e+00
Identity = 735/1051 (69.93%), Postives = 791/1051 (75.26%), Query Frame = 0
Query: 1 MKNAAVKGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLECGKEIENPHMD 60
M NA+VKGLEVFDFTEEDELPELISE+ LSKFKNPN+E N VLKY LE GKE+ENPHMD
Sbjct: 1 MNNASVKGLEVFDFTEEDELPELISERCLSKFKNPNLEINTVLKYGCLELGKEVENPHMD 60
Query: 61 VDLDECNRGCDDGISHIPSGSTKEQFIMKEEKYQLDANTESELNSHSQDVFMQVDNHVTG 120
VDLDECNRGCD+GISHIPSG +KEQ IM+EEKYQLD NTESE N+HSQD+F+QV+NHVTG
Sbjct: 61 VDLDECNRGCDNGISHIPSGISKEQLIMEEEKYQLDVNTESEWNTHSQDMFVQVNNHVTG 120
Query: 121 SLGSDSELGKIGSSSQSPTLGLNCTLPEFATERKQVDAISDPNGSMNGSSPMSSPSEIVE 180
G SELGK+GSSSQS LGLNCTLPEFA +R+QVDA+S PNGSMNGSSPMSSPSE+VE
Sbjct: 121 CFG--SELGKVGSSSQSSILGLNCTLPEFAAKREQVDALSYPNGSMNGSSPMSSPSELVE 180
Query: 181 DGVLLNGKSSDKCSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFSNNGIKINGFAEY 240
D V NG+SSDKCSSDNEMDDLN+EVVLCPDY++YGD YC S LTFS+NGIKINGF++Y
Sbjct: 181 DSVSPNGRSSDKCSSDNEMDDLNQEVVLCPDYIIYGDSYCTSSQLTFSHNGIKINGFSDY 240
Query: 241 GSDEFLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACDTSGQWTSASIKE 300
GS+EFLNLEWRVDDL++IEC WFQRVE+V IKLH ISKD GQ DNAC TSG +KE
Sbjct: 241 GSNEFLNLEWRVDDLVNIECHWFQRVEFVTIKLHTISKDVGQRDNACGTSG------LKE 300
Query: 301 VKIALVESHWSEKQQKIRSLDSRYMAIWNMSLFAGIGTDDDDLGGQRHYFPYFDEHFEEV 360
VKI LV+ WSEKQQKIRSLDSRYMAIWNMSL AG DDDLGG R YFP FDEHFEEV
Sbjct: 301 VKIVLVDPCWSEKQQKIRSLDSRYMAIWNMSLDAG----DDDLGGPRQYFPNFDEHFEEV 360
Query: 361 VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKHRFHFFNSFFFRK 420
VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLK QIDPKEKHRFHFFNSFFFRK
Sbjct: 361 VYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPKEKHRFHFFNSFFFRK 420
Query: 421 LADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHPGEVARY 480
LADLDKDPSSASDG+AAFLRVRKWTRKV+LFDKDYIFIPINF+LHWSLMVICHPGEVARY
Sbjct: 421 LADLDKDPSSASDGKAAFLRVRKWTRKVNLFDKDYIFIPINFNLHWSLMVICHPGEVARY 480
Query: 481 SDEDLMKSMKVPCILHMDSIKGSHAAGLATLLAMEHQLYSGLTLGLPTLLWPLGIPTYTV 540
SDE+L+KS KVPCILHMDSIKGSHA GL L+
Sbjct: 481 SDENLIKSTKVPCILHMDSIKGSHA-------------------GLKNLI---------- 540
Query: 541 FHKAKRRQLKRFGIFKDEEAIKWADLSLDVAPIVLSHEEDSWGWNIHPSRQFSTKSLLSD 600
Sbjct: 541 ------------------------------------------------------------ 600
Query: 601 MSDSRCLLESSLTKGIWKDNYPKKIKFFLWEISHRAISTKDVLQKRMPYLAISPDENSVP 660
Sbjct: 601 ------------------------------------------------------------ 660
Query: 661 SYKRSYLLEEWKERNKETSEDISSKFKNLRFLPLELNVDWFPPAEAYLKRTLIQRLIFEI 720
+SYLLEEW ERNK+ SEDISSKFKNLRFLPLELNVDWFPPAEAYLKRTLIQRLIFEI
Sbjct: 661 ---QSYLLEEWNERNKDASEDISSKFKNLRFLPLELNVDWFPPAEAYLKRTLIQRLIFEI 720
Query: 721 LENRSREMSTAACSDELLSKFPSNNEDETGVELLPERGSPAVTCNHNLSSSQAVDGIEIT 780
LENRSREMST ACSD+ LSKFPSNNEDE GVEL+PE G A T +HNLSSSQA DGIEIT
Sbjct: 721 LENRSREMSTTACSDD-LSKFPSNNEDEAGVELVPESGRLAETFDHNLSSSQAADGIEIT 780
Query: 781 LLSESSSRHNHFLDGSGLVVRELFEPGASNGSLLGHYQSFAQTSSYFDANGTVLEEDADA 840
LLSESSSRHNHF+DGSGLVVRELFEPG SNGSLLGHYQ FAQTSSYFD N TVLEEDAD
Sbjct: 781 LLSESSSRHNHFIDGSGLVVRELFEPGTSNGSLLGHYQPFAQTSSYFDTNVTVLEEDADT 840
Query: 841 ETEDRFMYLPPEQDGLQPTDAMTSQACRFPCSSRGLESEAAFDLCMSIQPEHGGGSASSP 900
ET DRFMYLP EQDGLQP DAMTSQACRFP SSRGLESE AFDLCMSIQPEHG G ASSP
Sbjct: 841 ETGDRFMYLPSEQDGLQPVDAMTSQACRFPGSSRGLESETAFDLCMSIQPEHGSGIASSP 885
Query: 901 SSHADHLADVGIIESCDVREASPGNKEEMSRKRPLSTENLEQPVAECPTSPAATMLDVDA 960
SSH+D L D+GIIESCDVRE SPGNKEE+ RKRPL ENLE P+AECPTS A T D D
Sbjct: 901 SSHSDVLEDMGIIESCDVREPSPGNKEEIIRKRPLPIENLE-PIAECPTSAATTTQDTDT 885
Query: 961 IVISQDANAICEDMDNNGSDPPCKETLVASPRLDEDITTKADIKHDDALVVASVVEVDLD 1020
IV+S+D N CEDM+N+GSDP KET+ ASP LDEDI+TK D++HDDA+V ASV EVDL
Sbjct: 961 IVVSKDTNDTCEDMENDGSDPHRKETVFASPCLDEDISTKTDVEHDDAVVDASVTEVDLH 885
Query: 1021 EQPAAKKPRHSLYLEEPSNGLKEGLSDDADL 1052
EQP AKKPRH Y EE SNGL + L +DADL
Sbjct: 1021 EQPPAKKPRHFPYPEEASNGLGDVLLEDADL 885
BLAST of Spg034115 vs. ExPASy TrEMBL
Match:
A0A6J1HC75 (probable ubiquitin-like-specific protease 2B isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462674 PE=3 SV=1)
HSP 1 Score: 1386.7 bits (3588), Expect = 0.0e+00
Identity = 761/1106 (68.81%), Postives = 808/1106 (73.06%), Query Frame = 0
Query: 1 MKNAAVKGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLEC---------- 60
MKNA+VKGLEVFDFTEEDELPELISEKRLSKFKNPN++TN VLKYEFLEC
Sbjct: 1 MKNASVKGLEVFDFTEEDELPELISEKRLSKFKNPNLDTNAVLKYEFLECDKLTVANGFV 60
Query: 61 --GKEIENPHMDVDLDECNRGCDDGISHIPSGSTKEQFIMKEEKYQLDANTESELNSHSQ 120
GKEI+NPHMDVDL +CNRGCD+GISHIPSG+TKEQFIM+E KYQLDANTESELNSHSQ
Sbjct: 61 VEGKEIKNPHMDVDLGKCNRGCDNGISHIPSGTTKEQFIMEEGKYQLDANTESELNSHSQ 120
Query: 121 DVFMQVDNHVTGSLGSDSELGKIGSSSQSPTLGLNCTLPEFATERKQVDAISDPNGSMNG 180
D+F+QVD HVTGSLG SEL KIGSSSQSP LGLNCTLPEF ER+ +DA+SDPNGSMNG
Sbjct: 121 DLFVQVDGHVTGSLG--SELDKIGSSSQSPILGLNCTLPEFNAEREPIDALSDPNGSMNG 180
Query: 181 SSPMSSPSEIVEDGVLLNGKSSDKCSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFS 240
+S MS PSE VEDGVLLNGK D CSSDNEMDDLNKEVVL PDY+VYGDFYCASP LTFS
Sbjct: 181 NSSMSPPSETVEDGVLLNGKYLDNCSSDNEMDDLNKEVVLYPDYIVYGDFYCASPSLTFS 240
Query: 241 NNGIKINGFAEYGSDEFLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACD 300
N IKINGFA+YGS+EFLNLEWRVDDLIDIECQ FQRVEYVMIKLHVISKDAGQCDN+CD
Sbjct: 241 PNAIKINGFADYGSNEFLNLEWRVDDLIDIECQCFQRVEYVMIKLHVISKDAGQCDNSCD 300
Query: 301 TSGQWTSASIKEVKIALVESHWSEKQQKIRSLDSRYMAIWNMSLFAGIGTDDDDLGGQRH 360
TSG IKEVKI LV+S+WSEKQQKIRSLDSRYMAIWNMSL AGIGTDDDDLGGQRH
Sbjct: 301 TSG------IKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNMSLDAGIGTDDDDLGGQRH 360
Query: 361 YFPYFDEHFEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKH 420
YFP FDE FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLK QIDP++KH
Sbjct: 361 YFPNFDEPFEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPQDKH 420
Query: 421 RFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSL 480
RFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLF+KDYIFIPINF+LHWSL
Sbjct: 421 RFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFEKDYIFIPINFNLHWSL 480
Query: 481 MVICHPGEVARYSDEDLMKSMKVPCILHMDSIKGSHAAGLATLLAMEHQLYSGLTLGLPT 540
MVICH GEVARYSDEDL KSMKVPCILHMDSIKGSHA GL
Sbjct: 481 MVICHLGEVARYSDEDLTKSMKVPCILHMDSIKGSHA-------------------GLKN 540
Query: 541 LLWPLGIPTYTVFHKAKRRQLKRFGIFKDEEAIKWADLSLDVAPIVLSHEEDSWGWNIHP 600
L+
Sbjct: 541 LI---------------------------------------------------------- 600
Query: 601 SRQFSTKSLLSDMSDSRCLLESSLTKGIWKDNYPKKIKFFLWEISHRAISTKDVLQKRMP 660
Sbjct: 601 ------------------------------------------------------------ 660
Query: 661 YLAISPDENSVPSYKRSYLLEEWKERNKETSEDISSKFKNLRFLPLE------------- 720
+SYLLEEWKERNKET EDIS+KFKNLRFLPLE
Sbjct: 661 ---------------QSYLLEEWKERNKETPEDISTKFKNLRFLPLELPQQENSFDCGLF 720
Query: 721 ---------------------------LNVDWFPPAEAYLKRTLIQRLIFEILENRSREM 780
LNVDWFPPAEAYLKRTLIQRLIFEILENRSREM
Sbjct: 721 LLHYLELFLAEAPLSFSPFKIFKLSKFLNVDWFPPAEAYLKRTLIQRLIFEILENRSREM 780
Query: 781 STAACSDELLSKFPSNNEDETGVELLPERGSPAVTCNHNLSSSQAVDGIEITLLSESSSR 840
STAACSDELLSKFPSNNEDETGVELL ERGSPAV CNHNLSSSQAVDGIEITLLSESSSR
Sbjct: 781 STAACSDELLSKFPSNNEDETGVELLLERGSPAVACNHNLSSSQAVDGIEITLLSESSSR 840
Query: 841 HNHFLDGSGLVVRELFEPGASNGSLLGHYQSFAQTSSYFDANGTVLEEDADAETEDRFMY 900
NHF+DGSGLVVRELFEPGASNGSLLG YQSFAQTSSYFD N TVLE+DADAET DRFM+
Sbjct: 841 PNHFIDGSGLVVRELFEPGASNGSLLGDYQSFAQTSSYFDTNATVLEDDADAETGDRFMF 900
Query: 901 LPPEQDGLQPTDAMTSQACRFPCSSRGLESEAAFDLCMSIQPEHGGGSASSPSSHADHLA 960
LPP QDGLQP DAM SQACRFPCSS GLES+A+FDLCMSIQPE G G A SS A+ L
Sbjct: 901 LPPVQDGLQPIDAMASQACRFPCSSIGLESKASFDLCMSIQPERGRGIA---SSSAEDLE 935
Query: 961 DVGIIESCDVREASPGNKEEMSRKRPLS--TENLEQPVAECPTSPAATMLDVDAIVISQD 1020
DVGIIESCDVREASPGNKE+ +RKRPLS ENLE PV E PTS A T+ DVD IVISQD
Sbjct: 961 DVGIIESCDVREASPGNKEDRNRKRPLSHEQENLE-PVTERPTSAATTVQDVDTIVISQD 935
Query: 1021 ANAICEDMDNNGSDPPCK-ETLVASPRLDEDITTKADIKHDDALVVASVVEVDLDEQPAA 1052
N M +N S+P C+ +TLVASP LDE TK D++HDD VV S+ EV+LDE+P A
Sbjct: 1021 TN-----MGSNDSEPLCEDDTLVASPCLDE--ATKTDVEHDDLPVVVSITEVNLDEEPPA 935
BLAST of Spg034115 vs. TAIR 10
Match:
AT1G09730.2 (Cysteine proteinases superfamily protein )
HSP 1 Score: 487.3 bits (1253), Expect = 3.2e-137
Identity = 364/1114 (32.68%), Postives = 515/1114 (46.23%), Query Frame = 0
Query: 7 KGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLE--CG-KEIENPHMD--- 66
K EVFDF EEDEL E + K L KF NP+ +PVL+ + ++ C K +E M+
Sbjct: 3 KNFEVFDFKEEDELAESAAGKLLEKFTNPSPCNSPVLQRQRIQSFCNEKRVEEEEMEGPS 62
Query: 67 -----VDLDECNRGCDDGISHIPSGSTKEQFI-------------------------MKE 126
++ + C+D + + + +K
Sbjct: 63 CAEPATAVESDDHQCEDDSTLVTEAKESRTILTFGLETTDHLEETDAEHVNQGLMLGLKT 122
Query: 127 EKYQLDANTESE-------LNSHSQDVFMQVDNHVTG---SLGSDSELGKIGSSSQSPTL 186
E + + + + LNS VD+ V LG +S + S SQ
Sbjct: 123 EDLAKETDIDHDNHGLMFGLNSEDDIEETDVDHRVESFSCQLGGNSFYAETSSYSQRQ-- 182
Query: 187 GLNCTLPEFATERKQVDAISDPNGSMNGSSPMSSPSEIVEDGVLLNGKSSDKCSSDNEMD 246
LN + ++ +Q+D +S + S++ S +S S+ +D ++ C +D E
Sbjct: 183 -LNSPFSDSSSSEEQIDMMSAIDESLSDRSALSEASDSEDDE---EDWMTEHCFNDEEKI 242
Query: 247 DLNKEVVLCPDYVVYGDFYCASPLLTFSNNGIKINGFAEYGSDEFLNLEWRVDDLIDIEC 306
DL+ V++ +YV+ D +CA+ L+ FS NGIKI F + + E+ V+D++ I+
Sbjct: 243 DLSTAVIMTSEYVILKDMHCAASLVIFSCNGIKIKSFLANNEEVPFSCEFGVEDIVSIQY 302
Query: 307 QWFQRVEYVMIKLHVISKDAGQCDNACDTSGQWTSASIKEVKIALVESHWSEKQQKIRSL 366
W+Q V +++++ V+ KD ++E+KIA+ E +W KQQKI SL
Sbjct: 303 NWYQNVGLIILRIRVLLKDEN------------CHEDMEELKIAVKEHNWPNKQQKINSL 362
Query: 367 DSRYMAIWNMSLFAGIGTDDDDLGGQRHYFPYFDEHFEEVVYPKGDPDAVSISKRDVDLL 426
+Y A+WN L + +L Q+ YFP FDE FE+VVYPKGDPDAVSI KRDV+LL
Sbjct: 363 HVKYPAVWNTDLEDDVEVSGYNLNQQKRYFPSFDEPFEDVVYPKGDPDAVSICKRDVELL 422
Query: 427 QPETFVNDTIIDFYIQYLKIQIDPKEKHRFHFFNSFFFRKLADLDKDPSSASDGRAAFLR 486
QPETFVNDTIIDFYI YLK QI +EKHRFHFFNSFFFRKLADLDKDPSS +DG+AAFLR
Sbjct: 423 QPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIADGKAAFLR 482
Query: 487 VRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHPGEVARYSDEDLMKSMKVPCILHMDSI 546
VRKWTRKVD+F KDYIF+P+N++LHWSL+VICHPGEVA +D DL S KVPCILHMDSI
Sbjct: 483 VRKWTRKVDMFGKDYIFVPVNYNLHWSLIVICHPGEVANRTDLDLDDSKKVPCILHMDSI 542
Query: 547 KGSHAAGLATLLAMEHQLYSGLTLGLPTLLWPLGIPTYTVFHKAKRRQLKRFGIFKDEEA 606
KGSHA GL L+
Sbjct: 543 KGSHA-------------------GLKNLV------------------------------ 602
Query: 607 IKWADLSLDVAPIVLSHEEDSWGWNIHPSRQFSTKSLLSDMSDSRCLLESSLTKGIWKDN 666
Sbjct: 603 ------------------------------------------------------------ 662
Query: 667 YPKKIKFFLWEISHRAISTKDVLQKRMPYLAISPDENSVPSYKRSYLLEEWKERNKETSE 726
++YL EEWKER+KETS+
Sbjct: 663 -------------------------------------------QTYLCEEWKERHKETSD 722
Query: 727 DISSKFKNLRFLPLE----------------------------------------LNVDW 786
DISS+F NLRF+ LE L ++W
Sbjct: 723 DISSRFMNLRFVSLELPQQENSFDCGLFLLHYLELFLAEAPLNFSPFKIYNASNFLYLNW 782
Query: 787 FPPAEAYLKRTLIQRLIFEILENRSREMSTAACSDELLSKFPSNNEDETGVELLPERGSP 846
FPPAEA LKRTLIQ+LIFE+LENRSRE+S + P D+ G+E+L ER SP
Sbjct: 783 FPPAEASLKRTLIQKLIFELLENRSREVSN---EQNQSCESPVAVNDDMGIEVLSERCSP 842
Query: 847 AVTCNHNLSSSQAVDGIEITLLSESSSRHNHFLDGSGLVVRELFEPGASN-GSLLGHYQS 906
+ CN +++ +Q GIE+TLL SS RH + SG+V+R+LF+ G++N GSLL Q
Sbjct: 843 LIDCNGDMTQTQDDQGIEMTLLERSSMRHIQAANDSGMVLRDLFDSGSNNTGSLLEQLQQ 902
Query: 907 -FAQTSSYFD-ANGTVLEEDADAETEDRFMYLPPEQDGLQPTDAMTSQACRFPCSSRGLE 966
F SS++ +N + E D ET ++FM L + Q T S R
Sbjct: 903 PFEDPSSFYHLSNDSSAREQVDMETGEQFMCLNAGEGNFQCITETT--------SPRASN 920
Query: 967 SEAAFDLCMSIQPEHGGGSASSPSSHADHLADVGIIESCDVREASPGNKEEMSRKRPLST 1026
S ++++L + + + S +S++ + +GIIE + ++EE+ P T
Sbjct: 963 SFSSWNLGIPLVQKEDETDLLSETSNSSN-DSIGIIEDNPIENT---HEEEIDESPPRET 920
Query: 1027 ENLEQPVAECPTSPAATMLDVDAIVISQDANAICEDMDNNGSDPPCKETLVASPRLDEDI 1032
+L+ T+ T + + V + + ++ + P + L + ED+
Sbjct: 1023 VSLKSATVGSNTADHTT--ENEEPVSTHIELVVPSSQNDRDEEKPLEHDLEIGDKTSEDV 920
BLAST of Spg034115 vs. TAIR 10
Match:
AT1G09730.1 (Cysteine proteinases superfamily protein )
HSP 1 Score: 481.9 bits (1239), Expect = 1.3e-135
Identity = 370/1143 (32.37%), Postives = 520/1143 (45.49%), Query Frame = 0
Query: 7 KGLEVFDFTEEDELPELISEKRLSKFKNPNIETNPVLKYEFLE--CG-KEIENPHMD--- 66
K EVFDF EEDEL E + K L KF NP+ +PVL+ + ++ C K +E M+
Sbjct: 3 KNFEVFDFKEEDELAESAAGKLLEKFTNPSPCNSPVLQRQRIQSFCNEKRVEEEEMEGPS 62
Query: 67 -----VDLDECNRGCDDGISHIPSGSTKEQFI-------------------------MKE 126
++ + C+D + + + +K
Sbjct: 63 CAEPATAVESDDHQCEDDSTLVTEAKESRTILTFGLETTDHLEETDAEHVNQGLMLGLKT 122
Query: 127 EKYQLDANTESE-------LNSHSQDVFMQVDNHVTG---SLGSDSELGKIGSSSQSPTL 186
E + + + + LNS VD+ V LG +S + S SQ
Sbjct: 123 EDLAKETDIDHDNHGLMFGLNSEDDIEETDVDHRVESFSCQLGGNSFYAETSSYSQRQ-- 182
Query: 187 GLNCTLPEFATERKQVDAISDPNGSMNGSSPMS--SPSEIVEDGV-----------LLNG 246
LN + ++ +Q+D +S + S++ S +S S SE E+G +L
Sbjct: 183 -LNSPFSDSSSSEEQIDMMSAIDESLSDRSALSEASDSEDDEEGTCYTFSLYFLIDMLGS 242
Query: 247 KSSDK----------------CSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFSNNG 306
SD+ C +D E DL+ V++ +YV+ D +CA+ L+ FS NG
Sbjct: 243 PMSDRVLISMLYALKDWMTEHCFNDEEKIDLSTAVIMTSEYVILKDMHCAASLVIFSCNG 302
Query: 307 IKINGFAEYGSDEFLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACDTSG 366
IKI F + + E+ V+D++ I+ W+Q V +++++ V+ KD
Sbjct: 303 IKIKSFLANNEEVPFSCEFGVEDIVSIQYNWYQNVGLIILRIRVLLKDEN---------- 362
Query: 367 QWTSASIKEVKIALVESHWSEKQQKIRSLDSRYMAIWNMSLFAGIGTDDDDLGGQRHYFP 426
++E+KIA+ E +W KQQKI SL +Y A+WN L + +L Q+ YFP
Sbjct: 363 --CHEDMEELKIAVKEHNWPNKQQKINSLHVKYPAVWNTDLEDDVEVSGYNLNQQKRYFP 422
Query: 427 YFDEHFEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKHRFH 486
FDE FE+VVYPKGDPDAVSI KRDV+LLQPETFVNDTIIDFYI YLK QI +EKHRFH
Sbjct: 423 SFDEPFEDVVYPKGDPDAVSICKRDVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFH 482
Query: 487 FFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVI 546
FFNSFFFRKLADLDKDPSS +DG+AAFLRVRKWTRKVD+F KDYIF+P+N++LHWSL+VI
Sbjct: 483 FFNSFFFRKLADLDKDPSSIADGKAAFLRVRKWTRKVDMFGKDYIFVPVNYNLHWSLIVI 542
Query: 547 CHPGEVARYSDEDLMKSMKVPCILHMDSIKGSHAAGLATLLAMEHQLYSGLTLGLPTLLW 606
CHPGEVA +D DL S KVPCILHMDSIKGSHA GL L+
Sbjct: 543 CHPGEVANRTDLDLDDSKKVPCILHMDSIKGSHA-------------------GLKNLV- 602
Query: 607 PLGIPTYTVFHKAKRRQLKRFGIFKDEEAIKWADLSLDVAPIVLSHEEDSWGWNIHPSRQ 666
Sbjct: 603 ------------------------------------------------------------ 662
Query: 667 FSTKSLLSDMSDSRCLLESSLTKGIWKDNYPKKIKFFLWEISHRAISTKDVLQKRMPYLA 726
Sbjct: 663 ------------------------------------------------------------ 722
Query: 727 ISPDENSVPSYKRSYLLEEWKERNKETSEDISSKFKNLRFLPLE---------------- 786
++YL EEWKER+KETS+DISS+F NLRF+ LE
Sbjct: 723 ------------QTYLCEEWKERHKETSDDISSRFMNLRFVSLELPQQENSFDCGLFLLH 782
Query: 787 ------------------------LNVDWFPPAEAYLKRTLIQRLIFEILENRSREMSTA 846
L ++WFPPAEA LKRTLIQ+LIFE+LENRSRE+S
Sbjct: 783 YLELFLAEAPLNFSPFKIYNASNFLYLNWFPPAEASLKRTLIQKLIFELLENRSREVSN- 842
Query: 847 ACSDELLSKFPSNNEDETGVELLPERGSPAVTCNHNLSSSQAVDGIEITLLSESSSRHNH 906
+ P D+ G+E+L ER SP + CN +++ +Q GIE+TLL SS RH
Sbjct: 843 --EQNQSCESPVAVNDDMGIEVLSERCSPLIDCNGDMTQTQDDQGIEMTLLERSSMRHIQ 902
Query: 907 FLDGSGLVVRELFEPGASN-GSLLGHYQS-FAQTSSYFD-ANGTVLEEDADAETEDRFMY 966
+ SG+V+R+LF+ G++N GSLL Q F SS++ +N + E D ET ++FM
Sbjct: 903 AANDSGMVLRDLFDSGSNNTGSLLEQLQQPFEDPSSFYHLSNDSSAREQVDMETGEQFMC 952
Query: 967 LPPEQDGLQPTDAMTSQACRFPCSSRGLESEAAFDLCMSIQPEHGGGSASSPSSHADHLA 1026
L + Q T S R S ++++L + + + S +S++ +
Sbjct: 963 LNAGEGNFQCITETT--------SPRASNSFSSWNLGIPLVQKEDETDLLSETSNSSN-D 952
Query: 1027 DVGIIESCDVREASPGNKEEMSRKRPLSTENLEQPVAECPTSPAATMLDVDAIVISQDAN 1032
+GIIE + ++EE+ P T +L+ T+ T + + V +
Sbjct: 1023 SIGIIEDNPIENT---HEEEIDESPPRETVSLKSATVGSNTADHTT--ENEEPVSTHIEL 952
BLAST of Spg034115 vs. TAIR 10
Match:
AT4G33620.1 (Cysteine proteinases superfamily protein )
HSP 1 Score: 301.2 bits (770), Expect = 3.2e-81
Identity = 185/515 (35.92%), Postives = 274/515 (53.20%), Query Frame = 0
Query: 7 KGLEVFDFTEEDELPELISEKRLSKFKNPNIETN--PVLKYEFLECGKEIENPHMDVDLD 66
K ++VFD+++ED+ E S+K L KF +P + + + KYEFL C
Sbjct: 13 KPIDVFDYSDEDDRVEEESKKLLRKFDSPVTKKHHCAIDKYEFLRC-------------- 72
Query: 67 ECNRGCDDGISHIPSGSTKEQFIMKEEKYQLDANTESELNSHSQDVFMQVDNHVTGSLGS 126
+ D +ES++ H + + V+ V S
Sbjct: 73 ----------------------------FAKDTQSESKVLQH---IVIDVEVPVKEE-PS 132
Query: 127 DSELGKIGSSSQSPTLGLNCTLPEFATERKQVDAISDPNGSMNGSSPMSSPSEIVEDGVL 186
EL G+S +D IS+ + G ++S S + E+ +
Sbjct: 133 RCELSGDGNSD-------------------LIDVISNGSHRRIGIDSLTS-SSLSENDEV 192
Query: 187 LNGKSSDKCSSDNEMDDLNKEVVLCPDYVVYGDFYCASPLLTFSNNGIKINGFAEYGSDE 246
G++++ S +E+D N +V++ PD ++YGD YC + LTFS N + + + +
Sbjct: 193 STGEATNPASDPHEVDPENAQVLIIPDVIIYGDIYCTNSKLTFSRNCMNVESSSVNATKG 252
Query: 247 FLNLEWRVDDLIDIECQWFQRVEYVMIKLHVISKDAGQCDNACDTSGQWTSASIKEVKIA 306
+ +W ++D+I IE QW VE + + + S+ D A D SG I +K +
Sbjct: 253 TFSCQWTIEDIIKIESQWCLEVETAFVNVLLKSRKPEGVDIAKDISG------IDLLKFS 312
Query: 307 LVESHWSEKQQKIRSLDSRYMAIW--NMSLFAGIGTDDDDLGGQRHYFPYFDEHFEEVVY 366
+ + WS++ + IRSLDSRY IW ++ I DLG + FE++VY
Sbjct: 313 VYDPKWSKEVETIRSLDSRYKNIWFDTITESEEIAFSGHDLGTS---LTNLADSFEDLVY 372
Query: 367 PKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEKHRFHFFNSFFFRKLA 426
P+G+PDAV + K+D++LL+P F+NDTIIDFYI+YLK +I PKE+ RFHFFN FFFRKLA
Sbjct: 373 PQGEPDAVVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKERGRFHFFNCFFFRKLA 432
Query: 427 DLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHPGEVA---- 486
+LDK S GR A+ RV+KWT+ VDLF+KDYIFIPIN S HWSL++ICHPGE+
Sbjct: 433 NLDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCSFHWSLVIICHPGELVPSHV 452
Query: 487 --RYSDEDLMKSMKVPCILHMDSIKGSHAAGLATL 512
D+++ +VPCILH+DSIKGSH GL +
Sbjct: 493 NFHSFDDEVENPQRVPCILHLDSIKGSHKGGLINI 452
BLAST of Spg034115 vs. TAIR 10
Match:
AT1G60220.1 (UB-like protease 1D )
HSP 1 Score: 98.6 bits (244), Expect = 3.2e-20
Identity = 54/151 (35.76%), Postives = 90/151 (59.60%), Query Frame = 0
Query: 358 EEVVYP-KGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEK--HRFHFFN 417
E++ YP + DP V + +D++ L P ++ +++FY+++L+ QI + HFFN
Sbjct: 330 EDICYPTRDDPHFVQVCLKDLECLAPREYLTSPVMNFYMRFLQQQISSSNQISADCHFFN 389
Query: 418 SFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVICHP 477
++F++KL+ D +D A F+R R+W + +DLF K YIFIPI+ LHWSL+++C P
Sbjct: 390 TYFYKKLS--DAVTYKGNDKDAFFVRFRRWWKGIDLFRKAYIFIPIHEDLHWSLVIVCIP 449
Query: 478 GEVARYSDEDLMKSMKVPCILHMDSIKGSHA 506
+ DE + ILH+DS+ G H+
Sbjct: 450 DK----KDESGL------TILHLDSL-GLHS 467
BLAST of Spg034115 vs. TAIR 10
Match:
AT1G10570.1 (Cysteine proteinases superfamily protein )
HSP 1 Score: 96.3 bits (238), Expect = 1.6e-19
Identity = 56/157 (35.67%), Postives = 87/157 (55.41%), Query Frame = 0
Query: 358 EEVVYPKGDP----DAVSISKRDVDLLQPETFVNDTIIDFYIQYLKIQIDPKEK--HRFH 417
E++ YP D D V +S +D+ L P ++ +I+FYI+Y++ + +K H
Sbjct: 315 EDIYYPSSDQSDGRDLVQVSLKDLKCLSPGEYLTSPVINFYIRYVQHHVFSADKTAANCH 374
Query: 418 FFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFSLHWSLMVI 477
FFN+FF++KL + +D A F++ R+W + DLF K YIFIPI+ LHWSL++I
Sbjct: 375 FFNTFFYKKLTEAVS--YKGNDRDAYFVKFRRWWKGFDLFCKSYIFIPIHEDLHWSLVII 434
Query: 478 CHPGEVARYSDEDLMKSMKVPCILHMDSIKGSHAAGL 509
C P + DE + I+H+DS+ G H L
Sbjct: 435 CIPDK----EDESGL------TIIHLDSL-GLHPRNL 458
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_023546149.1 | 0.0e+00 | 71.46 | probable ubiquitin-like-specific protease 2B isoform X3 [Cucurbita pepo subsp. p... | [more] |
XP_022962129.1 | 0.0e+00 | 71.39 | probable ubiquitin-like-specific protease 2B isoform X3 [Cucurbita moschata] | [more] |
XP_022951344.1 | 0.0e+00 | 70.31 | probable ubiquitin-like-specific protease 2B isoform X2 [Cucurbita moschata] | [more] |
XP_023546148.1 | 0.0e+00 | 69.62 | probable ubiquitin-like-specific protease 2B isoform X2 [Cucurbita pepo subsp. p... | [more] |
XP_022962128.1 | 0.0e+00 | 69.56 | probable ubiquitin-like-specific protease 2B isoform X2 [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
Q8L7S0 | 4.5e-136 | 32.68 | Probable ubiquitin-like-specific protease 2B OS=Arabidopsis thaliana OX=3702 GN=... | [more] |
Q0WKV8 | 1.3e-79 | 36.35 | Probable ubiquitin-like-specific protease 2A OS=Arabidopsis thaliana OX=3702 GN=... | [more] |
O13769 | 1.2e-19 | 38.73 | Ubiquitin-like-specific protease 2 OS=Schizosaccharomyces pombe (strain 972 / AT... | [more] |
Q6P7W0 | 2.6e-19 | 35.21 | Sentrin-specific protease 6 OS=Mus musculus OX=10090 GN=Senp6 PE=1 SV=3 | [more] |
Q2PS26 | 4.5e-19 | 35.76 | Ubiquitin-like-specific protease 1D OS=Arabidopsis thaliana OX=3702 GN=ULP1D PE=... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1HG30 | 0.0e+00 | 71.39 | probable ubiquitin-like-specific protease 2B isoform X3 OS=Cucurbita moschata OX... | [more] |
A0A6J1GIE5 | 0.0e+00 | 70.31 | probable ubiquitin-like-specific protease 2B isoform X2 OS=Cucurbita moschata OX... | [more] |
A0A6J1HDX0 | 0.0e+00 | 69.56 | probable ubiquitin-like-specific protease 2B isoform X2 OS=Cucurbita moschata OX... | [more] |
A0A6J1KRR7 | 0.0e+00 | 69.93 | probable ubiquitin-like-specific protease 2B isoform X2 OS=Cucurbita maxima OX=3... | [more] |
A0A6J1HC75 | 0.0e+00 | 68.81 | probable ubiquitin-like-specific protease 2B isoform X1 OS=Cucurbita moschata OX... | [more] |