Spg034062 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg034062
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptiontranscription factor bHLH96
Locationscaffold13: 34142235 .. 34144164 (+)
RNA-Seq ExpressionSpg034062
SyntenySpg034062
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCTCTTCTCCTTCTTTCTTTCTTTCTTTCTTTCAAATTTCCCTCTCTTTCCACGAAACAGAGCAACCCCATCTCTCTTTACTTTCTTCTCTCTGTTTTTATGATCCACAAATCCAAAAGCAGAGAGATTAAACAGAGTGATCGTTCATGATGGCATTGGAAACTGTGGTTTACCCACAAGATACATTCAGTTATGTTTGCAGAGATTTCTGTTCTTACTCTGGAGCTTGGCCCGCCTACGATTACGCCTTCCAATTAGAGCAAGAAAAAACCTTTCTTGGTGACGACCAGAAATTGCCCGCTAATTGGGACGCTTCACCGGACGAATGTTCCATCAATCAGCCGCTCCCCGGAGGTCCTCTGTACCCGCCGGTGGAAGTCGAACCCACGCCGCCACCGCCGCCGCCGACCACCGGCAGGAGAAAGCGGCGGCGGACTCGGAGCACGAAAAACAAGGAGGAAATTGAAAACCAGAGGATGACCCACATCGCCGTCGAGCGCAACCGCCGGAAACAAATGAATGATTATCTGGCCGTCCTCCGATCACTAATGCCGTCTTCTTACGTTCAAAGGGTCAGTCACCTTTCTTTCTCATAACCCCCAAAAAAAAGAAAAAGAAAAAAGAAAAAGATTATAATGATAATGACAAAAGGTACCGCCCAAAAGTCTCTGATCTCAAAAAAGAGTATCATATCAAATCTCTCCCTCAATTTTCATATATTTTTTTTTCTCTTTACTTTTAGAGGGATGGAACTGTTCAATTACTTCAATTTCACGACATAACGATTGCATGTTTAATAATTGGGTAGGGGGGGTTGTGTTATTTTGTTGTATTATGGCACATGCAATGTCAATTTTGAACCCCAGAGGCCTAATTATTTGCATATTATAGCAGGGAGACCAAGCCTCCATTATTGGTGGCGCCATTAACTTTGTCAAGGAATTAGAGCAGCAATTGCAATTCATCAAGGCCCATAAGGAAACAGCTTCTTCCTCCCCAATTGAAAATAACGCTTCTCCTTTCGCCGACTTCTTTTCCTTTCCTCAGTTCTCAACTCGTGCGACTCAGTGCAAGAACTCGGGCCATGACTCGGCCATGCCGGCTCACACCCAATGGGCCGCCGGAGATATTGAAGTCACCATGGTCGACAGCCATGCCAACCTTAAAATACTGTCCCAGAAACGCCCTAGGCAGCTTCTCAAAATGGTGGCTGGGTTTCAAAGCTTGAGACTCACTATTCTTCATCTCAATGTCACCACTTTGGATCAAATGGTTCTTTACTCTGTGAGCATCAAGGTAAAAAGTTTGAAACTTTATGAACTTTTAGAACATGGGTTGCTTTTAATTTTTGGTTTTGTCAAAAGTAGTGATGAGTATTTTTGAATTCTTTTTTGGGCTTTCAGGTTGAGGAAGGATGCCAGTTGAATACAGTGGATGAAATTGCTGCAGCTGTGAATCAAATTCTACTTTCCATACAAGAGGAAGCTGCTTTCAGCTGACACTTTGAAAAAAAAAAACCCAAAATACAAAAAGTAAAAAAAAAAAAAAAGGAAAAAAAAAGTTTTTTTTTTTTTCTTTTGTTCCTATGATTACTTGTGGAAGCGAAGCGATGGATCGATTTAATCAGTTTCTTTGGAATGTATTTTGTTTGTTCAGAATATCTTCGAGTTTCGTATGGTGTCCAACAATTTTCCATGTAAAAGAAGCACATAGCGTTGGCTCCTTATGATCTGTTAAATTTAATTTCATGTAATTGGCAATTACGTGCTACCACCGGCTTTGTTCAAAGATGAAGCTTTTTTGGACGTTTTAAAAAAAATATATATGAAAGCTGAAAACTCAATCGTACACATGAACGATTCCAACTTTATTTAAATAATCTTATGATTTTTGTCTCATCATTCCCTATGAGTTATAAGTAATTGGAG

mRNA sequence

ATGATGGCATTGGAAACTGTGGTTTACCCACAAGATACATTCAGTTATGTTTGCAGAGATTTCTGTTCTTACTCTGGAGCTTGGCCCGCCTACGATTACGCCTTCCAATTAGAGCAAGAAAAAACCTTTCTTGGTGACGACCAGAAATTGCCCGCTAATTGGGACGCTTCACCGGACGAATGTTCCATCAATCAGCCGCTCCCCGGAGGTCCTCTGTACCCGCCGGTGGAAGTCGAACCCACGCCGCCACCGCCGCCGCCGACCACCGGCAGGAGAAAGCGGCGGCGGACTCGGAGCACGAAAAACAAGGAGGAAATTGAAAACCAGAGGATGACCCACATCGCCGTCGAGCGCAACCGCCGGAAACAAATGAATGATTATCTGGCCGTCCTCCGATCACTAATGCCGTCTTCTTACGTTCAAAGGGGAGACCAAGCCTCCATTATTGGTGGCGCCATTAACTTTGTCAAGGAATTAGAGCAGCAATTGCAATTCATCAAGGCCCATAAGGAAACAGCTTCTTCCTCCCCAATTGAAAATAACGCTTCTCCTTTCGCCGACTTCTTTTCCTTTCCTCAGTTCTCAACTCGTGCGACTCAGTGCAAGAACTCGGGCCATGACTCGGCCATGCCGGCTCACACCCAATGGGCCGCCGGAGATATTGAAGTCACCATGGTCGACAGCCATGCCAACCTTAAAATACTGTCCCAGAAACGCCCTAGGCAGCTTCTCAAAATGGTGGCTGGGTTTCAAAGCTTGAGACTCACTATTCTTCATCTCAATGTCACCACTTTGGATCAAATGGTTCTTTACTCTGTGAGCATCAAGGTTGAGGAAGGATGCCAGTTGAATACAGTGGATGAAATTGCTGCAGCTGTGAATCAAATTCTACTTTCCATACAAGAGGAAGCTGCTTTCAGCTGA

Coding sequence (CDS)

ATGATGGCATTGGAAACTGTGGTTTACCCACAAGATACATTCAGTTATGTTTGCAGAGATTTCTGTTCTTACTCTGGAGCTTGGCCCGCCTACGATTACGCCTTCCAATTAGAGCAAGAAAAAACCTTTCTTGGTGACGACCAGAAATTGCCCGCTAATTGGGACGCTTCACCGGACGAATGTTCCATCAATCAGCCGCTCCCCGGAGGTCCTCTGTACCCGCCGGTGGAAGTCGAACCCACGCCGCCACCGCCGCCGCCGACCACCGGCAGGAGAAAGCGGCGGCGGACTCGGAGCACGAAAAACAAGGAGGAAATTGAAAACCAGAGGATGACCCACATCGCCGTCGAGCGCAACCGCCGGAAACAAATGAATGATTATCTGGCCGTCCTCCGATCACTAATGCCGTCTTCTTACGTTCAAAGGGGAGACCAAGCCTCCATTATTGGTGGCGCCATTAACTTTGTCAAGGAATTAGAGCAGCAATTGCAATTCATCAAGGCCCATAAGGAAACAGCTTCTTCCTCCCCAATTGAAAATAACGCTTCTCCTTTCGCCGACTTCTTTTCCTTTCCTCAGTTCTCAACTCGTGCGACTCAGTGCAAGAACTCGGGCCATGACTCGGCCATGCCGGCTCACACCCAATGGGCCGCCGGAGATATTGAAGTCACCATGGTCGACAGCCATGCCAACCTTAAAATACTGTCCCAGAAACGCCCTAGGCAGCTTCTCAAAATGGTGGCTGGGTTTCAAAGCTTGAGACTCACTATTCTTCATCTCAATGTCACCACTTTGGATCAAATGGTTCTTTACTCTGTGAGCATCAAGGTTGAGGAAGGATGCCAGTTGAATACAGTGGATGAAATTGCTGCAGCTGTGAATCAAATTCTACTTTCCATACAAGAGGAAGCTGCTTTCAGCTGA

Protein sequence

MMALETVVYPQDTFSYVCRDFCSYSGAWPAYDYAFQLEQEKTFLGDDQKLPANWDASPDECSINQPLPGGPLYPPVEVEPTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMTHIAVERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKETASSSPIENNASPFADFFSFPQFSTRATQCKNSGHDSAMPAHTQWAAGDIEVTMVDSHANLKILSQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVNQILLSIQEEAAFS
Homology
BLAST of Spg034062 vs. NCBI nr
Match: XP_023536809.1 (transcription factor bHLH96-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 482.3 bits (1240), Expect = 3.2e-132
Identity = 259/312 (83.01%), Postives = 278/312 (89.10%), Query Frame = 0

Query: 1   MMALETVVYPQ--DTFSYVCRDFCSYSGAWPAYDYAFQLEQEKTFLGDDQKLPANWDASP 60
           MMALETVVYPQ  DTF+YVCRDFCSY GAWP YDY FQLEQEK+FL + QKLP NW+ SP
Sbjct: 1   MMALETVVYPQNRDTFTYVCRDFCSYGGAWPGYDYGFQLEQEKSFLENPQKLPTNWEPSP 60

Query: 61  DECSINQPLPGGPLYPPVEVE---PTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMTHIA 120
           +ECSINQPL  G LYPP+EVE   P+PP PPPTTGRRKRRRTR+ KNKEEIENQRMTHIA
Sbjct: 61  EECSINQPLSVGALYPPMEVEQSPPSPPSPPPTTGRRKRRRTRNMKNKEEIENQRMTHIA 120

Query: 121 VERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKETASS 180
           VERNRRKQMN+YLAVLRSLMP SYVQRGDQASIIGGAINFVKELEQQLQFIKAHKE A+S
Sbjct: 121 VERNRRKQMNEYLAVLRSLMPCSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKEPAAS 180

Query: 181 SPIENNASPFADFFSFPQFSTRATQCKNSGHDSAMPAHTQWAAGDIEVTMVDSHANLKIL 240
                  SPFADFFSFPQFST AT   NS HDSA  ++TQWAAGDIEVTMVD+HANLKIL
Sbjct: 181 -------SPFADFFSFPQFSTPAT---NSTHDSA-SSNTQWAAGDIEVTMVDTHANLKIL 240

Query: 241 SQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVNQ 300
           S+KRPRQLLKMVAGFQSLR ++LHLNVTTLDQMVLYSVSIK+EEGC+LNTVDEIAAAVNQ
Sbjct: 241 SKKRPRQLLKMVAGFQSLRFSVLHLNVTTLDQMVLYSVSIKIEEGCELNTVDEIAAAVNQ 300

Query: 301 ILLSIQEEAAFS 308
           ILLSIQEE+AFS
Sbjct: 301 ILLSIQEESAFS 301

BLAST of Spg034062 vs. NCBI nr
Match: XP_038884224.1 (transcription factor bHLH96 [Benincasa hispida])

HSP 1 Score: 481.5 bits (1238), Expect = 5.5e-132
Identity = 262/318 (82.39%), Postives = 277/318 (87.11%), Query Frame = 0

Query: 1   MMALETVVYPQDTFSYVCRDFCSYSGA--WPAYDYAFQLEQEKTFLG------DDQKLPA 60
           MMALETVVYPQDTFSYVCRDFCSY G   W +YDY FQLE++K FLG      +DQKL  
Sbjct: 1   MMALETVVYPQDTFSYVCRDFCSYGGGGPWGSYDYGFQLEEQKAFLGVIQNQTEDQKLAT 60

Query: 61  NWDASPDECSINQPLPGGPLYPPVEVEPTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMT 120
           +W+ASP+ECSINQPL GG LYPPVEVE  PPPP PTTGRRKRRRTRSTKNKEEIENQRMT
Sbjct: 61  SWEASPEECSINQPLSGGALYPPVEVE--PPPPLPTTGRRKRRRTRSTKNKEEIENQRMT 120

Query: 121 HIAVERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKET 180
           HIAVERNRRKQMN+YL+VLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIK HKE 
Sbjct: 121 HIAVERNRRKQMNEYLSVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKVHKE- 180

Query: 181 ASSSPIENNASPFADFFSFPQFSTRAT---QCKNSGHDSAMPAHTQWAAGDIEVTMVDSH 240
                 +   SPFADFFSFPQ+STRAT   +  NS HDS++ AHTQW AGDIEVTMVDSH
Sbjct: 181 ------QTGTSPFADFFSFPQYSTRATTQSKNNNSPHDSSI-AHTQWPAGDIEVTMVDSH 240

Query: 241 ANLKILSQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEI 300
           ANLKILSQKRPRQLLKMVAGFQSLRLTILHLNVTT DQMVLYS+SIKVEEGCQLNTVDEI
Sbjct: 241 ANLKILSQKRPRQLLKMVAGFQSLRLTILHLNVTTSDQMVLYSLSIKVEEGCQLNTVDEI 300

Query: 301 AAAVNQILLSIQEEAAFS 308
           AAAVNQILLSIQEEA FS
Sbjct: 301 AAAVNQILLSIQEEAVFS 308

BLAST of Spg034062 vs. NCBI nr
Match: KAG6585364.1 (Transcription factor basic helix-loop-helix 96, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 480.7 bits (1236), Expect = 9.3e-132
Identity = 258/312 (82.69%), Postives = 277/312 (88.78%), Query Frame = 0

Query: 1   MMALETVVYPQ--DTFSYVCRDFCSYSGAWPAYDYAFQLEQEKTFLGDDQKLPANWDASP 60
           MMALETVVYPQ  DTF+YVCRDFCSY GAWP YDY FQLEQEK+FL + QKLP NW+ SP
Sbjct: 1   MMALETVVYPQNRDTFTYVCRDFCSYGGAWPGYDYGFQLEQEKSFLENPQKLPTNWEPSP 60

Query: 61  DECSINQPLPGGPLYPPVEVE---PTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMTHIA 120
           +ECSINQPL  G LYPP+EVE   P+PP PPPTTGRRKRRRTR+ KNKEEIENQRMTHIA
Sbjct: 61  EECSINQPLSVGALYPPMEVEQSPPSPPSPPPTTGRRKRRRTRNMKNKEEIENQRMTHIA 120

Query: 121 VERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKETASS 180
           VERNRRKQMN+YLAVLRSLMP SYVQRGDQASIIGGAINFVKELEQQLQFIKAHKE  +S
Sbjct: 121 VERNRRKQMNEYLAVLRSLMPCSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKEPTAS 180

Query: 181 SPIENNASPFADFFSFPQFSTRATQCKNSGHDSAMPAHTQWAAGDIEVTMVDSHANLKIL 240
                  SPFADFFSFPQFST AT   NS HDSA  ++TQWAAGDIEVTMVD+HANLKIL
Sbjct: 181 -------SPFADFFSFPQFSTPAT---NSTHDSA-SSNTQWAAGDIEVTMVDTHANLKIL 240

Query: 241 SQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVNQ 300
           S+KRPRQLLKMVAGFQSLR ++LHLNVTTLDQMVLYSVSIK+EEGC+LNTVDEIAAAVNQ
Sbjct: 241 SKKRPRQLLKMVAGFQSLRFSVLHLNVTTLDQMVLYSVSIKIEEGCELNTVDEIAAAVNQ 300

Query: 301 ILLSIQEEAAFS 308
           ILLSIQEE+AFS
Sbjct: 301 ILLSIQEESAFS 301

BLAST of Spg034062 vs. NCBI nr
Match: XP_023002448.1 (transcription factor bHLH96-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 478.4 bits (1230), Expect = 4.6e-131
Identity = 257/312 (82.37%), Postives = 276/312 (88.46%), Query Frame = 0

Query: 1   MMALETVVYPQ--DTFSYVCRDFCSYSGAWPAYDYAFQLEQEKTFLGDDQKLPANWDASP 60
           MMALETVVYPQ  DTF+YVCRDFCSY  AWP YDY FQLEQEK+FL + QKLP NW+ SP
Sbjct: 1   MMALETVVYPQNRDTFTYVCRDFCSYGSAWPGYDYGFQLEQEKSFLENPQKLPTNWEPSP 60

Query: 61  DECSINQPLPGGPLYPPVEVE---PTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMTHIA 120
           +ECSINQPL  G LYPP+EVE   P+PP PPPTTGRRKRRRTR+ KNKEEIENQRMTHIA
Sbjct: 61  EECSINQPLSVGALYPPMEVEQSPPSPPSPPPTTGRRKRRRTRNMKNKEEIENQRMTHIA 120

Query: 121 VERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKETASS 180
           VERNRRKQMN+YLAVLRSLMP SYVQRGDQASIIGGAINFVKELEQQLQFIKAHKE  +S
Sbjct: 121 VERNRRKQMNEYLAVLRSLMPCSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKEPTAS 180

Query: 181 SPIENNASPFADFFSFPQFSTRATQCKNSGHDSAMPAHTQWAAGDIEVTMVDSHANLKIL 240
                  SPFADFFSFPQFST AT   NS HDSA  ++TQWAAGDIEVTMVD+HANLKIL
Sbjct: 181 -------SPFADFFSFPQFSTPAT---NSTHDSA-SSNTQWAAGDIEVTMVDTHANLKIL 240

Query: 241 SQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVNQ 300
           S+KRPRQLLKMVAGFQSLR ++LHLNVTTLDQMVLYSVSIK+EEGC+LNTVDEIAAAVNQ
Sbjct: 241 SKKRPRQLLKMVAGFQSLRFSVLHLNVTTLDQMVLYSVSIKIEEGCELNTVDEIAAAVNQ 300

Query: 301 ILLSIQEEAAFS 308
           ILLSIQEE+AFS
Sbjct: 301 ILLSIQEESAFS 301

BLAST of Spg034062 vs. NCBI nr
Match: KAG7020275.1 (Transcription factor bHLH96, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 478.4 bits (1230), Expect = 4.6e-131
Identity = 257/312 (82.37%), Postives = 276/312 (88.46%), Query Frame = 0

Query: 1   MMALETVVYPQ--DTFSYVCRDFCSYSGAWPAYDYAFQLEQEKTFLGDDQKLPANWDASP 60
           MMALETVVYPQ  DTF+YVCRDFCSY GAWP YDY FQLEQEK+FL + QKLP NW+ SP
Sbjct: 1   MMALETVVYPQNRDTFTYVCRDFCSYGGAWPGYDYGFQLEQEKSFLENPQKLPTNWEPSP 60

Query: 61  DECSINQPLPGGPLYPPVEVE---PTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMTHIA 120
           +ECSI QPL  G LYPP+EVE   P+PP PPPTTGRRKRRRTR+ KNKEEIENQRMTHIA
Sbjct: 61  EECSIKQPLSVGALYPPMEVEQSPPSPPSPPPTTGRRKRRRTRNMKNKEEIENQRMTHIA 120

Query: 121 VERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKETASS 180
           VERNRRKQMN+YLAVLRSLMP SYVQRGDQASIIGGAINFVKELEQQLQFIKAHKE  +S
Sbjct: 121 VERNRRKQMNEYLAVLRSLMPCSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKEPTAS 180

Query: 181 SPIENNASPFADFFSFPQFSTRATQCKNSGHDSAMPAHTQWAAGDIEVTMVDSHANLKIL 240
                  SPFADFFSFPQFST AT   NS HDSA  ++TQWAAGDIEVTMVD+HANLKIL
Sbjct: 181 -------SPFADFFSFPQFSTPAT---NSTHDSA-SSNTQWAAGDIEVTMVDTHANLKIL 240

Query: 241 SQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVNQ 300
           S+KRPRQLLKMVAGFQSLR ++LHLNVTTLDQMVLYSVSIK+EEGC+LNTVDEIAAAVNQ
Sbjct: 241 SKKRPRQLLKMVAGFQSLRFSVLHLNVTTLDQMVLYSVSIKIEEGCELNTVDEIAAAVNQ 300

Query: 301 ILLSIQEEAAFS 308
           ILLSIQEE+AFS
Sbjct: 301 ILLSIQEESAFS 301

BLAST of Spg034062 vs. ExPASy Swiss-Prot
Match: Q9C7T4 (Transcription factor bHLH96 OS=Arabidopsis thaliana OX=3702 GN=BHLH96 PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-73
Identity = 177/341 (51.91%), Postives = 222/341 (65.10%), Query Frame = 0

Query: 2   MALETVVYPQDTFSYV-CRDFCSYSGAWPAYDYAFQLEQEK------------------- 61
           MALE VVYPQD FSY+ C+DF       P YD  FQ E+++                   
Sbjct: 1   MALEAVVYPQDPFSYISCKDF-------PFYDLYFQEEEDQDPQDTKNNIKLGQGQGHGF 60

Query: 62  ---TFLGDDQKLPANWDASPDECSINQPLPGGPLYPPVEVEPTPPPPPPTT--GRRKRRR 121
               + G       +++ + ++    + LP G     V+ E  PPP       GRRKRRR
Sbjct: 61  ASNNYNGRTGDYSDDYNYNEEDLQWPRDLPYG---SAVDTESQPPPSDVAAGGGRRKRRR 120

Query: 122 TRSTKNKEEIENQRMTHIAVERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFV 181
           TRS+KNKEEIENQRMTHIAVERNRRKQMN+YLAVLRSLMP  Y QRGDQASI+GGAIN++
Sbjct: 121 TRSSKNKEEIENQRMTHIAVERNRRKQMNEYLAVLRSLMPPYYAQRGDQASIVGGAINYL 180

Query: 182 KELEQQLQFIKAHKETA----------SSSPIENNASPFADFFSFPQFSTRATQCKNSGH 241
           KELE  LQ ++   +TA          + +   +++ PF+DFF+FPQ+S R T       
Sbjct: 181 KELEHHLQSMEPPVKTATEDTGAGHDQTKTTSASSSGPFSDFFAFPQYSNRPT------- 240

Query: 242 DSAMPAHTQWAAGDIEVTMVDSHANLKILSQKRPRQLLKMVAGFQSLRLTILHLNVTTLD 301
            SA  A       +IEVTMV+SHA+LKIL++KRPRQLLK+V+  QSLRLT+LHLNVTT D
Sbjct: 241 -SAAAAE---GMAEIEVTMVESHASLKILAKKRPRQLLKLVSSIQSLRLTLLHLNVTTRD 300

Query: 302 QMVLYSVSIKVEEGCQLNTVDEIAAAVNQILLSIQEEAAFS 308
             VLYS+S+KVEEG QLNTV++IAAAVNQIL  I+EE++FS
Sbjct: 301 DSVLYSISVKVEEGSQLNTVEDIAAAVNQILRRIEEESSFS 320

BLAST of Spg034062 vs. ExPASy Swiss-Prot
Match: Q9SK91 (Transcription factor bHLH94 OS=Arabidopsis thaliana OX=3702 GN=BHLH94 PE=1 SV=2)

HSP 1 Score: 262.3 bits (669), Expect = 6.8e-69
Identity = 162/318 (50.94%), Postives = 209/318 (65.72%), Query Frame = 0

Query: 2   MALETVVYPQDTFSYV--CRDFCSYSGAWPAYDYAFQ--------LEQEKTFLGDDQKLP 61
           M LE VVYPQD F Y+  C+DF  +   +   ++  Q        L  E++F+   ++  
Sbjct: 1   MPLEAVVYPQDPFGYLSNCKDF-MFHDLYSQEEFVAQDTKNNIDKLGHEQSFVEQGKEDD 60

Query: 62  ANWDASPDECSINQPLPGGPL-YPPVEVEPTPPPPPPTTGRRKRRRTRSTKNKEEIENQR 121
             W     +  +  P  G  L    ++VE  PPP      RRKRRRTR+ KNKEEIENQR
Sbjct: 61  HQW-RDYHQYPLLIPSLGEELGLTAIDVESHPPP----QHRRKRRRTRNCKNKEEIENQR 120

Query: 122 MTHIAVERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFI---- 181
           MTHIAVERNRRKQMN+YLAVLRSLMPSSY QRGDQASI+GGAIN+VKELE  LQ +    
Sbjct: 121 MTHIAVERNRRKQMNEYLAVLRSLMPSSYAQRGDQASIVGGAINYVKELEHILQSMEPKR 180

Query: 182 -KAHKETASSSPIENNASPFADFFSFPQFSTRATQCKNSGHDSAMPAHTQWAAGDIEVTM 241
            + H      +   +   PF DFFSFPQ+ST+++   +    S+ PA       +IEVT+
Sbjct: 181 TRTHDPKGDKTSTSSLVGPFTDFFSFPQYSTKSS--SDVPESSSSPA-------EIEVTV 240

Query: 242 VDSHANLKILSQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNT 301
            +SHAN+KI+++K+PRQLLK++   QSLRLT+LHLNVTTL   +LYS+S++VEEG QLNT
Sbjct: 241 AESHANIKIMTKKKPRQLLKLITSLQSLRLTLLHLNVTTLHNSILYSISVRVEEGSQLNT 300

Query: 302 VDEIAAAVNQILLSIQEE 304
           VD+IA A+NQ +  IQEE
Sbjct: 301 VDDIATALNQTIRRIQEE 303

BLAST of Spg034062 vs. ExPASy Swiss-Prot
Match: Q56XR0 (Transcription factor bHLH71 OS=Arabidopsis thaliana OX=3702 GN=BHLH71 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.4e-45
Identity = 119/254 (46.85%), Postives = 159/254 (62.60%), Query Frame = 0

Query: 79  EPTPPPPPPTTGR---RKRRRTRSTKNKEEIENQRMTHIAVERNRRKQMNDYLAVLRSLM 138
           EP P   PP T R   R+RR+ R  KN+EE ENQRMTHIAVERNRR+QMN +L+VLRSLM
Sbjct: 53  EPPPQRQPPATNRGKKRRRRKPRVCKNEEEAENQRMTHIAVERNRRRQMNQHLSVLRSLM 112

Query: 139 PSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHK------------ETASSSPIENNAS 198
           P  +  +GDQASI+GGAI+F+KELE +L  ++A K             T+  S  E    
Sbjct: 113 PQPFAHKGDQASIVGGAIDFIKELEHKLLSLEAQKHHNAKLNQSVTSSTSQDSNGEQENP 172

Query: 199 PFADFFSFPQF---STRATQCKNSGHDSAMPAHTQWAAGDIEVTMVDSHANLKILSQKR- 258
                 S  QF   S   +Q   +G  S++    +    D+EVT++++HAN++ILS++R 
Sbjct: 173 HQPSSLSLSQFFLHSYDPSQENRNGSTSSVKTPME----DLEVTLIETHANIRILSRRRG 232

Query: 259 ----------PRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEI 304
                     P QL K+VA  QSL L+ILHL+VTTLD   +YS+S KVEE CQL++VD+I
Sbjct: 233 FRWSTLATTKPPQLSKLVASLQSLSLSILHLSVTTLDNYAIYSISAKVEESCQLSSVDDI 292

BLAST of Spg034062 vs. ExPASy Swiss-Prot
Match: Q9M128 (Transcription factor bHLH57 OS=Arabidopsis thaliana OX=3702 GN=BHLH57 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.3e-43
Identity = 101/225 (44.89%), Postives = 145/225 (64.44%), Query Frame = 0

Query: 91  RRKRRRTRSTKNKEEIENQRMTHIAVERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIG 150
           +RKR+RTR+ KNK+E+ENQRMTHIAVERNRR+QMN++L  LRSLMP S++QRGDQASI+G
Sbjct: 95  KRKRKRTRAPKNKDEVENQRMTHIAVERNRRRQMNEHLNSLRSLMPPSFLQRGDQASIVG 154

Query: 151 GAINFVKELEQQLQFIKAHKETASSSPIENNASPFADFFSFPQFSTRATQCKNSGHDSAM 210
           GAI+F+KELEQ LQ ++A K    +      AS           S+ +  C NS   S  
Sbjct: 155 GAIDFIKELEQLLQSLEAEKRKDGTDETPKTAS---------CSSSSSLACTNSSISSVS 214

Query: 211 PAH-----TQWAAGD---IEVTMVDSHANLKILSQKRPRQLLKMVAGFQSLRLTILHLNV 270
                    ++  GD   +E T++ +H +LK+  ++  RQ+LK +   + L+L ILHL +
Sbjct: 215 TTSENGFTARFGGGDTTEVEATVIQNHVSLKVRCKRGKRQILKAIVSIEELKLAILHLTI 274

Query: 271 TTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVNQILLSIQEEAAFS 308
           ++    V+YS ++K+E+GC+L + DEIA AV+QI   I  E  +S
Sbjct: 275 SSSFDFVIYSFNLKMEDGCKLGSADEIATAVHQIFEQINGEVMWS 310

BLAST of Spg034062 vs. ExPASy Swiss-Prot
Match: O81037 (Transcription factor bHLH70 OS=Arabidopsis thaliana OX=3702 GN=BHLH70 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.6e-41
Identity = 101/210 (48.10%), Postives = 138/210 (65.71%), Query Frame = 0

Query: 88  TTGRRKRRRTRSTKNKEEIENQRMTHIAVERNRRKQMNDYLAVLRSLMPSSYVQRGDQAS 147
           T  +RKRRRT+ TKN EEIE+QRMTHIAVERNRR+QMN +L  LRS++PSSY+QRGDQAS
Sbjct: 170 TREKRKRRRTKPTKNIEEIESQRMTHIAVERNRRRQMNVHLNSLRSIIPSSYIQRGDQAS 229

Query: 148 IIGGAINFVKELEQQLQFIKAHKETASSSPIENNASPFADFFSFPQFSTRATQCKNSGHD 207
           I+GGAI+FVK LEQQLQ ++A K +  S   ++N     +  S    S+   +  N    
Sbjct: 230 IVGGAIDFVKILEQQLQSLEAQKRSQQS---DDNKEQIPEDNSLRNISSNKLRASNKEEQ 289

Query: 208 SAMPAHTQWAAGDIEVTMVDSHANLKILSQKRPRQLLKMVAGFQSLRLTILHLNVTT-LD 267
           S+           IE T+++SH NLKI   ++  QLL+ +   + LR T+LHLN+T+  +
Sbjct: 290 SSKL--------KIEATVIESHVNLKIQCTRKQGQLLRSIILLEKLRFTVLHLNITSPTN 349

Query: 268 QMVLYSVSIKVEEGCQLNTVDEIAAAVNQI 297
             V YS ++K+E+ C L + DEI AA+ QI
Sbjct: 350 TSVSYSFNLKMEDECNLGSADEITAAIRQI 368

BLAST of Spg034062 vs. ExPASy TrEMBL
Match: A0A6J1KTK8 (transcription factor bHLH96-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496285 PE=4 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.2e-131
Identity = 257/312 (82.37%), Postives = 276/312 (88.46%), Query Frame = 0

Query: 1   MMALETVVYPQ--DTFSYVCRDFCSYSGAWPAYDYAFQLEQEKTFLGDDQKLPANWDASP 60
           MMALETVVYPQ  DTF+YVCRDFCSY  AWP YDY FQLEQEK+FL + QKLP NW+ SP
Sbjct: 1   MMALETVVYPQNRDTFTYVCRDFCSYGSAWPGYDYGFQLEQEKSFLENPQKLPTNWEPSP 60

Query: 61  DECSINQPLPGGPLYPPVEVE---PTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMTHIA 120
           +ECSINQPL  G LYPP+EVE   P+PP PPPTTGRRKRRRTR+ KNKEEIENQRMTHIA
Sbjct: 61  EECSINQPLSVGALYPPMEVEQSPPSPPSPPPTTGRRKRRRTRNMKNKEEIENQRMTHIA 120

Query: 121 VERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKETASS 180
           VERNRRKQMN+YLAVLRSLMP SYVQRGDQASIIGGAINFVKELEQQLQFIKAHKE  +S
Sbjct: 121 VERNRRKQMNEYLAVLRSLMPCSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKEPTAS 180

Query: 181 SPIENNASPFADFFSFPQFSTRATQCKNSGHDSAMPAHTQWAAGDIEVTMVDSHANLKIL 240
                  SPFADFFSFPQFST AT   NS HDSA  ++TQWAAGDIEVTMVD+HANLKIL
Sbjct: 181 -------SPFADFFSFPQFSTPAT---NSTHDSA-SSNTQWAAGDIEVTMVDTHANLKIL 240

Query: 241 SQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVNQ 300
           S+KRPRQLLKMVAGFQSLR ++LHLNVTTLDQMVLYSVSIK+EEGC+LNTVDEIAAAVNQ
Sbjct: 241 SKKRPRQLLKMVAGFQSLRFSVLHLNVTTLDQMVLYSVSIKIEEGCELNTVDEIAAAVNQ 300

Query: 301 ILLSIQEEAAFS 308
           ILLSIQEE+AFS
Sbjct: 301 ILLSIQEESAFS 301

BLAST of Spg034062 vs. ExPASy TrEMBL
Match: A0A6J1GKG5 (transcription factor bHLH96-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454739 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.1e-130
Identity = 257/312 (82.37%), Postives = 276/312 (88.46%), Query Frame = 0

Query: 1   MMALETVVYPQ--DTFSYVCRDFCSYSGAWPAYDYAFQLEQEKTFLGDDQKLPANWDASP 60
           MMALETVVYPQ  DTF+YV RDFCSY GAWP YDY FQLEQEK+FL + QKLP NW+ SP
Sbjct: 1   MMALETVVYPQNRDTFTYVGRDFCSYGGAWPGYDYGFQLEQEKSFLENPQKLPTNWEPSP 60

Query: 61  DECSINQPLPGGPLYPPVEVE---PTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMTHIA 120
           +ECSINQPL  G LYPP+EVE   P+PP PPPTTGRRKRRRTR+ KNKEEIENQRMTHIA
Sbjct: 61  EECSINQPLSVGALYPPMEVEQSPPSPPSPPPTTGRRKRRRTRNMKNKEEIENQRMTHIA 120

Query: 121 VERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKETASS 180
           VERNRRKQMN+YLAVLRSLMP SYVQRGDQASIIGGAINFVKELEQQLQFIKAHKE  +S
Sbjct: 121 VERNRRKQMNEYLAVLRSLMPCSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKEPTAS 180

Query: 181 SPIENNASPFADFFSFPQFSTRATQCKNSGHDSAMPAHTQWAAGDIEVTMVDSHANLKIL 240
                  SPFADFFSFPQFST AT   NS HDSA  ++TQWAAGDIEVTMVD+HANLKIL
Sbjct: 181 -------SPFADFFSFPQFSTPAT---NSTHDSA-SSNTQWAAGDIEVTMVDTHANLKIL 240

Query: 241 SQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVNQ 300
           S+KRPRQLLKMVAGFQSLR ++LHLNVTTLDQMVLYSVSIK+EEGC+LNTVDEIAAAVNQ
Sbjct: 241 SKKRPRQLLKMVAGFQSLRFSVLHLNVTTLDQMVLYSVSIKIEEGCELNTVDEIAAAVNQ 300

Query: 301 ILLSIQEEAAFS 308
           ILLSIQEE+AFS
Sbjct: 301 ILLSIQEESAFS 301

BLAST of Spg034062 vs. ExPASy TrEMBL
Match: A0A6J1KJJ2 (transcription factor bHLH96-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496285 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 5.5e-130
Identity = 257/313 (82.11%), Postives = 276/313 (88.18%), Query Frame = 0

Query: 1   MMALETVVYPQ--DTFSYVCRDFCSYSGAWPAYDYAFQLEQEKTFLGDDQKLPANWDASP 60
           MMALETVVYPQ  DTF+YVCRDFCSY  AWP YDY FQLEQEK+FL + QKLP NW+ SP
Sbjct: 1   MMALETVVYPQNRDTFTYVCRDFCSYGSAWPGYDYGFQLEQEKSFLENPQKLPTNWEPSP 60

Query: 61  DECSINQPLPGGPLYPPVEVE---PTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMTHIA 120
           +ECSINQPL  G LYPP+EVE   P+PP PPPTTGRRKRRRTR+ KNKEEIENQRMTHIA
Sbjct: 61  EECSINQPLSVGALYPPMEVEQSPPSPPSPPPTTGRRKRRRTRNMKNKEEIENQRMTHIA 120

Query: 121 VERNRRKQMNDYLAVLRSLMPSSYVQR-GDQASIIGGAINFVKELEQQLQFIKAHKETAS 180
           VERNRRKQMN+YLAVLRSLMP SYVQR GDQASIIGGAINFVKELEQQLQFIKAHKE  +
Sbjct: 121 VERNRRKQMNEYLAVLRSLMPCSYVQRQGDQASIIGGAINFVKELEQQLQFIKAHKEPTA 180

Query: 181 SSPIENNASPFADFFSFPQFSTRATQCKNSGHDSAMPAHTQWAAGDIEVTMVDSHANLKI 240
           S       SPFADFFSFPQFST AT   NS HDSA  ++TQWAAGDIEVTMVD+HANLKI
Sbjct: 181 S-------SPFADFFSFPQFSTPAT---NSTHDSA-SSNTQWAAGDIEVTMVDTHANLKI 240

Query: 241 LSQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVN 300
           LS+KRPRQLLKMVAGFQSLR ++LHLNVTTLDQMVLYSVSIK+EEGC+LNTVDEIAAAVN
Sbjct: 241 LSKKRPRQLLKMVAGFQSLRFSVLHLNVTTLDQMVLYSVSIKIEEGCELNTVDEIAAAVN 300

Query: 301 QILLSIQEEAAFS 308
           QILLSIQEE+AFS
Sbjct: 301 QILLSIQEESAFS 302

BLAST of Spg034062 vs. ExPASy TrEMBL
Match: A0A6J1BPY8 (transcription factor bHLH96 OS=Momordica charantia OX=3673 GN=LOC111004582 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 5.5e-130
Identity = 260/313 (83.07%), Postives = 281/313 (89.78%), Query Frame = 0

Query: 1   MMALETVVYPQDTFSYVCRDFCSYSGAWP-AYDYAFQLEQEKTFLG--DDQKLPANWDAS 60
           MMALETVVYPQDTFSYVCRDFCS+ GAW   YDY FQLE+++  LG  +DQKL +NW+AS
Sbjct: 1   MMALETVVYPQDTFSYVCRDFCSFGGAWEGGYDYGFQLEEQRALLGPIEDQKLGSNWEAS 60

Query: 61  PDECSIN-QPLPGGPLYPPVEVEPTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMTHIAV 120
           P+ECS + QPLPG  ++PPVE      PPPPTTGRRKRRRTRSTKNKEEIENQRMTHIAV
Sbjct: 61  PEECSTDQQPLPGA-VFPPVE------PPPPTTGRRKRRRTRSTKNKEEIENQRMTHIAV 120

Query: 121 ERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKETASSS 180
           ERNRRKQMN+YLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHK  A + 
Sbjct: 121 ERNRRKQMNEYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHK--AETQ 180

Query: 181 PIENNASPFADFFSFPQFSTRA-TQCKNSGHDSAMPAHTQW-AAGDIEVTMVDSHANLKI 240
           P  ++ASPFA+FFSFPQFSTRA TQCKNS +DS MP H QW AAGDIEVTMVD+HANLKI
Sbjct: 181 PQIDDASPFAEFFSFPQFSTRATTQCKNSANDSPMP-HQQWAAAGDIEVTMVDTHANLKI 240

Query: 241 LSQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVN 300
           LS+KRPRQLLKMVAGFQ+LRLT+LHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVN
Sbjct: 241 LSRKRPRQLLKMVAGFQTLRLTVLHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVN 300

Query: 301 QILLSIQEEAAFS 308
           QILLSIQEEAAFS
Sbjct: 301 QILLSIQEEAAFS 303

BLAST of Spg034062 vs. ExPASy TrEMBL
Match: A0A0A0LLQ6 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369200 PE=4 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 7.2e-130
Identity = 257/315 (81.59%), Postives = 275/315 (87.30%), Query Frame = 0

Query: 1   MMALETVVYPQDTFSYVCRDFCSYSGA--WPAYDYAFQLEQEKTFLG--DDQKLPANWDA 60
           MMALETVVYPQDTFSYVCRDF SY+G   W  YDYAFQ E++K FLG  ++Q     W+ 
Sbjct: 1   MMALETVVYPQDTFSYVCRDFSSYAGGPPWGTYDYAFQFEEQKAFLGLIENQTQDQKWEP 60

Query: 61  SPDECSINQPLP-GGPLYPPVEVEPTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMTHIA 120
           SPDE SIN PLP GG LYPP+E+EPTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMTHIA
Sbjct: 61  SPDESSINHPLPGGGSLYPPLELEPTPPPPPPTTGRRKRRRTRSTKNKEEIENQRMTHIA 120

Query: 121 VERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHKETASS 180
           VERNRRKQMN+YLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIK HKE    
Sbjct: 121 VERNRRKQMNEYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFIKVHKE---- 180

Query: 181 SPIENNASPFADFFSFPQFSTRAT---QCKNSGHDSAMPAHTQWAAGDIEVTMVDSHANL 240
              + + SPFADFFSFPQ+STRAT   +  NS HDS++ AHTQW AGDIEVTMVD+HANL
Sbjct: 181 ---QTDTSPFADFFSFPQYSTRATTQSKNNNSPHDSSI-AHTQWPAGDIEVTMVDTHANL 240

Query: 241 KILSQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEIAAA 300
           KILSQKRPRQLLKMVAGFQSLRLTILHLNVTT DQMVLYS+SIKVEEGCQLNTVDEIAAA
Sbjct: 241 KILSQKRPRQLLKMVAGFQSLRLTILHLNVTTSDQMVLYSLSIKVEEGCQLNTVDEIAAA 300

Query: 301 VNQILLSIQEEAAFS 308
           VNQILLSIQEEA +S
Sbjct: 301 VNQILLSIQEEAVYS 307

BLAST of Spg034062 vs. TAIR 10
Match: AT1G72210.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 278.1 bits (710), Expect = 8.5e-75
Identity = 177/341 (51.91%), Postives = 222/341 (65.10%), Query Frame = 0

Query: 2   MALETVVYPQDTFSYV-CRDFCSYSGAWPAYDYAFQLEQEK------------------- 61
           MALE VVYPQD FSY+ C+DF       P YD  FQ E+++                   
Sbjct: 1   MALEAVVYPQDPFSYISCKDF-------PFYDLYFQEEEDQDPQDTKNNIKLGQGQGHGF 60

Query: 62  ---TFLGDDQKLPANWDASPDECSINQPLPGGPLYPPVEVEPTPPPPPPTT--GRRKRRR 121
               + G       +++ + ++    + LP G     V+ E  PPP       GRRKRRR
Sbjct: 61  ASNNYNGRTGDYSDDYNYNEEDLQWPRDLPYG---SAVDTESQPPPSDVAAGGGRRKRRR 120

Query: 122 TRSTKNKEEIENQRMTHIAVERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFV 181
           TRS+KNKEEIENQRMTHIAVERNRRKQMN+YLAVLRSLMP  Y QRGDQASI+GGAIN++
Sbjct: 121 TRSSKNKEEIENQRMTHIAVERNRRKQMNEYLAVLRSLMPPYYAQRGDQASIVGGAINYL 180

Query: 182 KELEQQLQFIKAHKETA----------SSSPIENNASPFADFFSFPQFSTRATQCKNSGH 241
           KELE  LQ ++   +TA          + +   +++ PF+DFF+FPQ+S R T       
Sbjct: 181 KELEHHLQSMEPPVKTATEDTGAGHDQTKTTSASSSGPFSDFFAFPQYSNRPT------- 240

Query: 242 DSAMPAHTQWAAGDIEVTMVDSHANLKILSQKRPRQLLKMVAGFQSLRLTILHLNVTTLD 301
            SA  A       +IEVTMV+SHA+LKIL++KRPRQLLK+V+  QSLRLT+LHLNVTT D
Sbjct: 241 -SAAAAE---GMAEIEVTMVESHASLKILAKKRPRQLLKLVSSIQSLRLTLLHLNVTTRD 300

Query: 302 QMVLYSVSIKVEEGCQLNTVDEIAAAVNQILLSIQEEAAFS 308
             VLYS+S+KVEEG QLNTV++IAAAVNQIL  I+EE++FS
Sbjct: 301 DSVLYSISVKVEEGSQLNTVEDIAAAVNQILRRIEEESSFS 320

BLAST of Spg034062 vs. TAIR 10
Match: AT1G22490.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 4.9e-70
Identity = 162/318 (50.94%), Postives = 209/318 (65.72%), Query Frame = 0

Query: 2   MALETVVYPQDTFSYV--CRDFCSYSGAWPAYDYAFQ--------LEQEKTFLGDDQKLP 61
           M LE VVYPQD F Y+  C+DF  +   +   ++  Q        L  E++F+   ++  
Sbjct: 1   MPLEAVVYPQDPFGYLSNCKDF-MFHDLYSQEEFVAQDTKNNIDKLGHEQSFVEQGKEDD 60

Query: 62  ANWDASPDECSINQPLPGGPL-YPPVEVEPTPPPPPPTTGRRKRRRTRSTKNKEEIENQR 121
             W     +  +  P  G  L    ++VE  PPP      RRKRRRTR+ KNKEEIENQR
Sbjct: 61  HQW-RDYHQYPLLIPSLGEELGLTAIDVESHPPP----QHRRKRRRTRNCKNKEEIENQR 120

Query: 122 MTHIAVERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIGGAINFVKELEQQLQFI---- 181
           MTHIAVERNRRKQMN+YLAVLRSLMPSSY QRGDQASI+GGAIN+VKELE  LQ +    
Sbjct: 121 MTHIAVERNRRKQMNEYLAVLRSLMPSSYAQRGDQASIVGGAINYVKELEHILQSMEPKR 180

Query: 182 -KAHKETASSSPIENNASPFADFFSFPQFSTRATQCKNSGHDSAMPAHTQWAAGDIEVTM 241
            + H      +   +   PF DFFSFPQ+ST+++   +    S+ PA       +IEVT+
Sbjct: 181 TRTHDPKGDKTSTSSLVGPFTDFFSFPQYSTKSS--SDVPESSSSPA-------EIEVTV 240

Query: 242 VDSHANLKILSQKRPRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNT 301
            +SHAN+KI+++K+PRQLLK++   QSLRLT+LHLNVTTL   +LYS+S++VEEG QLNT
Sbjct: 241 AESHANIKIMTKKKPRQLLKLITSLQSLRLTLLHLNVTTLHNSILYSISVRVEEGSQLNT 300

Query: 302 VDEIAAAVNQILLSIQEE 304
           VD+IA A+NQ +  IQEE
Sbjct: 301 VDDIATALNQTIRRIQEE 303

BLAST of Spg034062 vs. TAIR 10
Match: AT5G46690.1 (beta HLH protein 71 )

HSP 1 Score: 184.9 bits (468), Expect = 9.8e-47
Identity = 119/254 (46.85%), Postives = 159/254 (62.60%), Query Frame = 0

Query: 79  EPTPPPPPPTTGR---RKRRRTRSTKNKEEIENQRMTHIAVERNRRKQMNDYLAVLRSLM 138
           EP P   PP T R   R+RR+ R  KN+EE ENQRMTHIAVERNRR+QMN +L+VLRSLM
Sbjct: 53  EPPPQRQPPATNRGKKRRRRKPRVCKNEEEAENQRMTHIAVERNRRRQMNQHLSVLRSLM 112

Query: 139 PSSYVQRGDQASIIGGAINFVKELEQQLQFIKAHK------------ETASSSPIENNAS 198
           P  +  +GDQASI+GGAI+F+KELE +L  ++A K             T+  S  E    
Sbjct: 113 PQPFAHKGDQASIVGGAIDFIKELEHKLLSLEAQKHHNAKLNQSVTSSTSQDSNGEQENP 172

Query: 199 PFADFFSFPQF---STRATQCKNSGHDSAMPAHTQWAAGDIEVTMVDSHANLKILSQKR- 258
                 S  QF   S   +Q   +G  S++    +    D+EVT++++HAN++ILS++R 
Sbjct: 173 HQPSSLSLSQFFLHSYDPSQENRNGSTSSVKTPME----DLEVTLIETHANIRILSRRRG 232

Query: 259 ----------PRQLLKMVAGFQSLRLTILHLNVTTLDQMVLYSVSIKVEEGCQLNTVDEI 304
                     P QL K+VA  QSL L+ILHL+VTTLD   +YS+S KVEE CQL++VD+I
Sbjct: 233 FRWSTLATTKPPQLSKLVASLQSLSLSILHLSVTTLDNYAIYSISAKVEESCQLSSVDDI 292

BLAST of Spg034062 vs. TAIR 10
Match: AT4G01460.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 178.3 bits (451), Expect = 9.2e-45
Identity = 101/225 (44.89%), Postives = 145/225 (64.44%), Query Frame = 0

Query: 91  RRKRRRTRSTKNKEEIENQRMTHIAVERNRRKQMNDYLAVLRSLMPSSYVQRGDQASIIG 150
           +RKR+RTR+ KNK+E+ENQRMTHIAVERNRR+QMN++L  LRSLMP S++QRGDQASI+G
Sbjct: 95  KRKRKRTRAPKNKDEVENQRMTHIAVERNRRRQMNEHLNSLRSLMPPSFLQRGDQASIVG 154

Query: 151 GAINFVKELEQQLQFIKAHKETASSSPIENNASPFADFFSFPQFSTRATQCKNSGHDSAM 210
           GAI+F+KELEQ LQ ++A K    +      AS           S+ +  C NS   S  
Sbjct: 155 GAIDFIKELEQLLQSLEAEKRKDGTDETPKTAS---------CSSSSSLACTNSSISSVS 214

Query: 211 PAH-----TQWAAGD---IEVTMVDSHANLKILSQKRPRQLLKMVAGFQSLRLTILHLNV 270
                    ++  GD   +E T++ +H +LK+  ++  RQ+LK +   + L+L ILHL +
Sbjct: 215 TTSENGFTARFGGGDTTEVEATVIQNHVSLKVRCKRGKRQILKAIVSIEELKLAILHLTI 274

Query: 271 TTLDQMVLYSVSIKVEEGCQLNTVDEIAAAVNQILLSIQEEAAFS 308
           ++    V+YS ++K+E+GC+L + DEIA AV+QI   I  E  +S
Sbjct: 275 SSSFDFVIYSFNLKMEDGCKLGSADEIATAVHQIFEQINGEVMWS 310

BLAST of Spg034062 vs. TAIR 10
Match: AT2G46810.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.1e-42
Identity = 101/210 (48.10%), Postives = 138/210 (65.71%), Query Frame = 0

Query: 88  TTGRRKRRRTRSTKNKEEIENQRMTHIAVERNRRKQMNDYLAVLRSLMPSSYVQRGDQAS 147
           T  +RKRRRT+ TKN EEIE+QRMTHIAVERNRR+QMN +L  LRS++PSSY+QRGDQAS
Sbjct: 170 TREKRKRRRTKPTKNIEEIESQRMTHIAVERNRRRQMNVHLNSLRSIIPSSYIQRGDQAS 229

Query: 148 IIGGAINFVKELEQQLQFIKAHKETASSSPIENNASPFADFFSFPQFSTRATQCKNSGHD 207
           I+GGAI+FVK LEQQLQ ++A K +  S   ++N     +  S    S+   +  N    
Sbjct: 230 IVGGAIDFVKILEQQLQSLEAQKRSQQS---DDNKEQIPEDNSLRNISSNKLRASNKEEQ 289

Query: 208 SAMPAHTQWAAGDIEVTMVDSHANLKILSQKRPRQLLKMVAGFQSLRLTILHLNVTT-LD 267
           S+           IE T+++SH NLKI   ++  QLL+ +   + LR T+LHLN+T+  +
Sbjct: 290 SSKL--------KIEATVIESHVNLKIQCTRKQGQLLRSIILLEKLRFTVLHLNITSPTN 349

Query: 268 QMVLYSVSIKVEEGCQLNTVDEIAAAVNQI 297
             V YS ++K+E+ C L + DEI AA+ QI
Sbjct: 350 TSVSYSFNLKMEDECNLGSADEITAAIRQI 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023536809.13.2e-13283.01transcription factor bHLH96-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_038884224.15.5e-13282.39transcription factor bHLH96 [Benincasa hispida][more]
KAG6585364.19.3e-13282.69Transcription factor basic helix-loop-helix 96, partial [Cucurbita argyrosperma ... [more]
XP_023002448.14.6e-13182.37transcription factor bHLH96-like isoform X2 [Cucurbita maxima][more]
KAG7020275.14.6e-13182.37Transcription factor bHLH96, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
Match NameE-valueIdentityDescription
Q9C7T41.2e-7351.91Transcription factor bHLH96 OS=Arabidopsis thaliana OX=3702 GN=BHLH96 PE=1 SV=1[more]
Q9SK916.8e-6950.94Transcription factor bHLH94 OS=Arabidopsis thaliana OX=3702 GN=BHLH94 PE=1 SV=2[more]
Q56XR01.4e-4546.85Transcription factor bHLH71 OS=Arabidopsis thaliana OX=3702 GN=BHLH71 PE=1 SV=1[more]
Q9M1281.3e-4344.89Transcription factor bHLH57 OS=Arabidopsis thaliana OX=3702 GN=BHLH57 PE=1 SV=1[more]
O810371.6e-4148.10Transcription factor bHLH70 OS=Arabidopsis thaliana OX=3702 GN=BHLH70 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KTK82.2e-13182.37transcription factor bHLH96-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1GKG51.1e-13082.37transcription factor bHLH96-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1KJJ25.5e-13082.11transcription factor bHLH96-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1BPY85.5e-13083.07transcription factor bHLH96 OS=Momordica charantia OX=3673 GN=LOC111004582 PE=4 ... [more]
A0A0A0LLQ67.2e-13081.59BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369200 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT1G72210.18.5e-7551.91basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G22490.14.9e-7050.94basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT5G46690.19.8e-4746.85beta HLH protein 71 [more]
AT4G01460.19.2e-4544.89basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G46810.11.1e-4248.10basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 156..176
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 48..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..88
NoneNo IPR availablePANTHERPTHR11969:SF26HELIX-LOOP-HELIX-LIKE PROTEINcoord: 2..305
NoneNo IPR availablePANTHERPTHR11969MAX DIMERIZATION, MADcoord: 2..305
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 114..165
e-value: 5.4E-10
score: 49.2
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 109..160
e-value: 2.1E-10
score: 40.5
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 108..159
score: 15.249706
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 92..174
e-value: 4.8E-12
score: 47.6
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 108..175

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg034062.1Spg034062.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0005634 nucleus
molecular_function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding