Spg033901 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg033901
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProteasome subunit beta
Locationscaffold13: 35919423 .. 35923240 (-)
RNA-Seq ExpressionSpg033901
SyntenySpg033901
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCCCTCCATTCTGATAATTTCCTGCGACGTTGTGTATATTTGCTTGGTCTAGTTCGCGTTTTTGTTCTCCCGTTGCTCTTGATTCTAACTTAAGGTAAAGTTGAACTAGTAAGATTTCGCTCTTTTCTGTTCTTGAGATTCATATGCGGATTCATGATACATTGTATATATGAATCAGTGTTTATCGTTAGGGTTTTAATGTCGACCTATTTTCTTAGTTGAAAATTGCTTGCATTCTGATAATTTTCCGGATCTGTTTTGATTTCTGATAGAAATTGAGGAGAGGTACCAAGATTTCGATTACCTAATTATATTTTTTTATGGTCAAGGAGCTTTAAGGGCCTTTTGATGTCGAATCAATTAGGATGACTATCTGGGTTTGGACGAGTGCTTTGGTAAATTTTTCAGGCTGGGTGTTCTAGATTAAGTTTTCATTAATTTTGTGAAATAGAACTAGCTCAGTCATCGGCATAGTTGGGGGTGTTTCTTGGGATTTTTTTTTTTCGAACTTTTATCTTGTTGTGTGGATAACTTCTTTGTAAGACGAACCCTGTAATTAAGCTTGATATTTGGTCATAACAGGAGTTGAAACATGAAGCTCGATACCAGTGGTCTTCAAATAACTGCTCCGCTGTTTGGGCCAAAAATGGAGATGCTGGAAGGGTTTTCAGCTGCTCCATCCTTTGAGATCCCTAATTCCAGTGACGTAATTTGTAAATCATTTCTCATAATGCTTTCTGGGCTGCATACTATTTTGTAGAATTGCTTTTTCTGACTTTGATTTTCTCCATATTTCAGTTTGATGGCTTTCAGAAAGAAGCCATTCAGATGGTGAAGCCAGCAAAAGGAACAACCACACTGGCTTTCATTTTTAAGGAAGGAGTCATGGTTGCTGCAGATTCGCGTGCCAGCATGGGAGGCTATATATGTATGTTTGCTTTATTTCATGTGTTCTTCGAACAAATTTAAGATTTTATTTCTAGATGAGTTTTGGTTTGGATACATGAACTGCAAAGTGCTATTCTAAGTTACTCTATCCCTTTTAGCGATTAGAGGTTTGTGACCCCCTAGATAGAATGCTCTGTGCAGTATGATGCATTTACTCGCATTTTCCTGTTTAGCTTTATATCGGTTGTCCTCCCTCCCCCCACCCTCCATTGTATTGTAAGTTATTGTGTTGTGTTATTTGAACTAAATATTTGTTTTTAAATCTGTTCAGCATCACAATCTGTGAAAAAAATTATTGAAATCAATCCTTACATGCTTGGCACAATGGCTGGAGGTGCTGCTGATTGCCAGTTTTGGCATAGAAACCTCGGTGTCAAGGTAATTGGCCAATATACAATTACTTGTAGAACTATCAGCTTTTATTGAGAATGGGTTATCCTGTATTACTATCATTGTGATTTCGTTATGTCTAATTTTGAGAGTTTTCCAGTCTCAACAATTCAACTTCTAAATGTGCAATCGGATATTTTCCCATCTTTATTCAATAAATTGTACCTTGCCGAAGTTGATATATTCTCATGTGGTTAGCTGATCATAGTGTTTAACATGATTTAGTGCCGCCGACATGAATTAGCAAATAAGCGCCGGATTTCAGTTGCAGGAGCATCAAAATTACTGGCCAACATTCTGTACTCCTATCGTGGAATGGGTCTCTCTGTTGGGACTATGATTGCTGGATGGGATGAAACAGTAATAACATATTTATCTATTTTTATTTTCTTATTTTAAAATGAAAATTGCAATGACATTTATTTATTTTCATTTTCTTATTTTATTATGATCTATAACTTACCTTCTTCTGTGCATATTAGGGTCCTGGTCTATATTACGTAGACAGTGAGGGAGGGAGGCTGAAAGGAACAAGATTTTCTGTTGGATCTGGTTCACCTTATGCCTATGGTGTCTTGGATAGTGGGTAAGTGTGATTCTTCATTATCAGGCTGTCTTCAATATTTCTCAATCCGGTAATTTTATTGTGCCTTATGCCTATGGTGTCTTGGATAGTGGGTAAGTGTGATTCTTCATTATCAGGCTGTCTTCAATATTTCTCAGTCGAGTAATTTTATTGTGAACTTCTTATCACATGTTAAGCTAAATAATGTTATTTTGTGAACCACCATGATTTGGCATGAGTTGGCCTGGTAATATTGGGGCATGCGGAAAAGAAAAGAGAACTCCATGGTGGCCATAAATTTGTAGAGTCCTAGGATATTTTATTTTAAAAAAAATCAAACGAACTGCCGACATTTTGGAAGAAATGTTTTGTTTTAAAAAGAAATTTTTTTTTATTGTTATTATTTTTATAAGAAACATGACTTTTCATTATCGAAAGAGATATGCAAAAAAGGGGAAGATGAGATATCCCCAGACACTAAAGGATTTCAAAAAAGCCTCCCAACTGGTAAGTAATTGAGCTATTATGGTATAATTACAAAATAAATTAGAATGGAAAGCCCATGTAGAAGCAAAGATTTCACAAGTTCCCAAAAAAAACTCCCTGTTTAAGCTTTGATCATGAAACACCCTCCTATTCCTTTCCAACCAGCAACCACACTATTGACCCGTAAAATTCTAGCTTGACCCTTAAAAGGATGACCATCAAGAAGCTGAAGGAGGACTTCTAAAGCACTGGAAGGAAACCATCAACCCCTATTGAAATAATGTTCAATTGATATGGTGAAAAGTTGTCATTGTCCTGAGGTAGCTAGTACAAAAGAAAGAAACAGGAAAACTTTTAACTCCCTGACTAAACTAATAGCAAATGAATTCGACACCCCTTCTCTGCAACATCTCTCCTTGTAAAAAGAAACTCAGTAGAAAGCTACATCCCAGCTTGTAATTGGGATTATATTTCTTGGTCTTGAAATTGCATTATTCCATCATCGAGGTAATAAACCACTTTGCCTGACCTGATGATGTTGGTTCAGACATTTCTTCAATATTCTTTCCTCTTACCTCTCATTTTGAAATAATTCATGAGTACTCTGTTGTGTTACAAAACAGATATCGTTATGACTTGTCTGTTGAAGAAGCTGCTGAACTGGCTAGACGAGCTATTTATCATGCAACTTTCCGAGATGGAGCTAGCGGTGGAGTCGCTAGCGGTATCCCTCTGTCTACTCTAGATGTTTCTTTGTGCTTGTAAAACATACATTTCTGTGCTTGTGCAAATCCTCTCCTCTCACTCGTAACTTGAATGTCGTTGATCAGTCTACTATGTTGGACCAAATGGATGGAAAAAGCTTTCTGGCGACGATGTAGGAGAACTTCATTACAATTACTATCCAGTGACGCCAACTACAGTGGATCAGGAAATGGCTGAAGTAACCATCGCTTGACAATCCCATATAAGACATGAGCAGGTTTAGACATTCTCTCCCTATTCATTTGTGCTACGGGAGGTACTGATTCTAAAGAGATTCTCTAGATGTGGACTTGTTGAAGTATATGTGTACACTCGCAGATCCTTAAAAAGTTTGATTGGATGTAGGTTTGGAAACTAGTGCTGCAAGGATCTTTCTGTCATGTAATTCGAGTTATGGCTGCTTGCTTTGAGGACATTGTCTATGTTGCCTAAATTTTAAATCCAGTTCTTCATTGGACTTCCTTTGTTATCAATGATTGTGTTTCCAGTTAGTAATATGGTCCTTGTATAACTAGAAAGTTGGTTGTTTAGTTTGTTTTAGTGATCCACTTAGTTTTTGTTAGAACAAATGAAATGAATCTATTATTATTTTTTAAAAAAAAGAAACATAGACTTTTGATGGTTGATGTACGATAATCTTGAATTTTTTGCAAGAGCTGGAC

mRNA sequence

ATGAAGCTCGATACCAGTGGTCTTCAAATAACTGCTCCGCTGTTTGGGCCAAAAATGGAGATGCTGGAAGGGTTTTCAGCTGCTCCATCCTTTGAGATCCCTAATTCCAGTGACTTTGATGGCTTTCAGAAAGAAGCCATTCAGATGGTGAAGCCAGCAAAAGGAACAACCACACTGGCTTTCATTTTTAAGGAAGGAGTCATGGTTGCTGCAGATTCGCGTGCCAGCATGGGAGGCTATATATCATCACAATCTGTGAAAAAAATTATTGAAATCAATCCTTACATGCTTGGCACAATGGCTGGAGGTGCTGCTGATTGCCAGTTTTGGCATAGAAACCTCGGTGTCAAGTGCCGCCGACATGAATTAGCAAATAAGCGCCGGATTTCAGTTGCAGGAGCATCAAAATTACTGGCCAACATTCTGTACTCCTATCGTGGAATGGGTCTCTCTGTTGGGACTATGATTGCTGGATGGGATGAAACAGGTCCTGGTCTATATTACGTAGACAGTGAGGGAGGGAGGCTGAAAGGAACAAGATTTTCTGTTGGATCTGGTTCACCTTATGCCTATGGTGTCTTGGATAGTGGATATCGTTATGACTTGTCTGTTGAAGAAGCTGCTGAACTGGCTAGACGAGCTATTTATCATGCAACTTTCCGAGATGGAGCTAGCGGTGGAGTCGCTAGCGTCTACTATGTTGGACCAAATGGATGGAAAAAGCTTTCTGGCGACGATGTAGGAGAACTTCATTACAATTACTATCCAGTGACGCCAACTACAGTGGATCAGGAAATGGCTGAAGTAACCATCGCTTGA

Coding sequence (CDS)

ATGAAGCTCGATACCAGTGGTCTTCAAATAACTGCTCCGCTGTTTGGGCCAAAAATGGAGATGCTGGAAGGGTTTTCAGCTGCTCCATCCTTTGAGATCCCTAATTCCAGTGACTTTGATGGCTTTCAGAAAGAAGCCATTCAGATGGTGAAGCCAGCAAAAGGAACAACCACACTGGCTTTCATTTTTAAGGAAGGAGTCATGGTTGCTGCAGATTCGCGTGCCAGCATGGGAGGCTATATATCATCACAATCTGTGAAAAAAATTATTGAAATCAATCCTTACATGCTTGGCACAATGGCTGGAGGTGCTGCTGATTGCCAGTTTTGGCATAGAAACCTCGGTGTCAAGTGCCGCCGACATGAATTAGCAAATAAGCGCCGGATTTCAGTTGCAGGAGCATCAAAATTACTGGCCAACATTCTGTACTCCTATCGTGGAATGGGTCTCTCTGTTGGGACTATGATTGCTGGATGGGATGAAACAGGTCCTGGTCTATATTACGTAGACAGTGAGGGAGGGAGGCTGAAAGGAACAAGATTTTCTGTTGGATCTGGTTCACCTTATGCCTATGGTGTCTTGGATAGTGGATATCGTTATGACTTGTCTGTTGAAGAAGCTGCTGAACTGGCTAGACGAGCTATTTATCATGCAACTTTCCGAGATGGAGCTAGCGGTGGAGTCGCTAGCGTCTACTATGTTGGACCAAATGGATGGAAAAAGCTTTCTGGCGACGATGTAGGAGAACTTCATTACAATTACTATCCAGTGACGCCAACTACAGTGGATCAGGAAATGGCTGAAGTAACCATCGCTTGA

Protein sequence

MKLDTSGLQITAPLFGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWKKLSGDDVGELHYNYYPVTPTTVDQEMAEVTIA
Homology
BLAST of Spg033901 vs. NCBI nr
Match: XP_038883961.1 (proteasome subunit beta type-5 isoform X1 [Benincasa hispida])

HSP 1 Score: 539.7 bits (1389), Expect = 1.5e-149
Identity = 266/272 (97.79%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLQITAPLFGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQ TAPLFGPKME LEGF+AAPSFEIPNSSDFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEALEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTIA 273
           KLSGDDVGELHYNYYPVTPTTVDQEMAEVT A
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTAA 272

BLAST of Spg033901 vs. NCBI nr
Match: XP_022961789.1 (proteasome subunit beta type-5-like [Cucurbita moschata] >XP_022997234.1 proteasome subunit beta type-5-like [Cucurbita maxima] >XP_022997235.1 proteasome subunit beta type-5-like [Cucurbita maxima])

HSP 1 Score: 539.3 bits (1388), Expect = 2.0e-149
Identity = 265/272 (97.43%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLQITAPLFGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQ TAPLFGPKME +EGF+AAPSFEIPNSSDFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEFMEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTIA 273
           KLSGDDVGELHYNYYPVTPTTVDQEMAEVT A
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTAA 272

BLAST of Spg033901 vs. NCBI nr
Match: XP_022131817.1 (proteasome subunit beta type-5 [Momordica charantia] >XP_022131818.1 proteasome subunit beta type-5 [Momordica charantia])

HSP 1 Score: 538.5 bits (1386), Expect = 3.3e-149
Identity = 265/270 (98.15%), Postives = 268/270 (99.26%), Query Frame = 0

Query: 1   MKLDTSGLQITAPLFGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQ TAPLFGPKME+LEGFSAAPSFEIPNS+DFDGFQKEAIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVT 271
           KLSGDDVGELHYNYYPVTP+TVDQEM EVT
Sbjct: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVT 270

BLAST of Spg033901 vs. NCBI nr
Match: XP_023002169.1 (proteasome subunit beta type-5-B isoform X1 [Cucurbita maxima])

HSP 1 Score: 536.6 bits (1381), Expect = 1.3e-148
Identity = 263/268 (98.13%), Postives = 267/268 (99.63%), Query Frame = 0

Query: 1   MKLDTSGLQITAPLFGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQ +APLFGPKME+LEGFSAAPSFE+PNSSDFDGFQKEAIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTSAPLFGPKMEILEGFSAAPSFEVPNSSDFDGFQKEAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAE 269
           KLSGDDVGELHYNYYPVTPTTVDQEMAE
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAE 268

BLAST of Spg033901 vs. NCBI nr
Match: XP_023538613.1 (proteasome subunit beta type-5-B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 536.6 bits (1381), Expect = 1.3e-148
Identity = 263/268 (98.13%), Postives = 267/268 (99.63%), Query Frame = 0

Query: 1   MKLDTSGLQITAPLFGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQ +APLFGPKME+LEGFSAAPSFE+PNSSDFDGFQKEAIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTSAPLFGPKMEILEGFSAAPSFEVPNSSDFDGFQKEAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAE 269
           KLSGDDVGELHYNYYPVTPTTVDQEMAE
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAE 268

BLAST of Spg033901 vs. ExPASy Swiss-Prot
Match: Q9LIP2 (Proteasome subunit beta type-5-B OS=Arabidopsis thaliana OX=3702 GN=PBE2 PE=1 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 3.5e-133
Identity = 229/270 (84.81%), Postives = 252/270 (93.33%), Query Frame = 0

Query: 1   MKLDTSGLQITAPL--FGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSGL+ T P+  FG   EML+GFS+APSF++P ++DFDGFQK+A++MVKPAKGTTT
Sbjct: 1   MKLDTSGLETTMPVIGFGSNSEMLDGFSSAPSFDLPRTTDFDGFQKKAVEMVKPAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKC 120
           LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KC
Sbjct: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG 180
           R HELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EGGRLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNG 240
            RFSVGSGSPYAYGVLDSGY++D+SVEEA+ELARR+IYHATFRDGASGGVASVY+VGP G
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPQG 240

Query: 241 WKKLSGDDVGELHYNYYPVTPTTVDQEMAE 269
           W KLSGDDVGELHY+YYPV P T +  M E
Sbjct: 241 WTKLSGDDVGELHYHYYPVAPITAEHVMEE 270

BLAST of Spg033901 vs. ExPASy Swiss-Prot
Match: O24361 (Proteasome subunit beta type-5 OS=Spinacia oleracea OX=3562 PE=2 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 2.9e-132
Identity = 225/269 (83.64%), Postives = 248/269 (92.19%), Query Frame = 0

Query: 1   MKLDTSGLQITAPLFGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGL+ TAP+F     + +G    PSF++PN +DFDGFQKEA+QMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLESTAPIFRRSDFVFDGLQMTPSFDLPNPTDFDGFQKEAVQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFK GVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KCR 
Sbjct: 61  FIFKHGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRL 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISV GASKLLANILY+YRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG R
Sbjct: 121 HELANKRRISVTGASKLLANILYNYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGMR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GY+YD++VEEA+ELARRAIYHAT+RDGASGGV SVY+VGP+GWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYKYDMTVEEASELARRAIYHATYRDGASGGVVSVYHVGPDGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEV 270
           K++GDDVG+LH+ YYPV P TV+QEM EV
Sbjct: 241 KVTGDDVGDLHFQYYPVVPATVEQEMVEV 269

BLAST of Spg033901 vs. ExPASy Swiss-Prot
Match: O23717 (Proteasome subunit beta type-5-A OS=Arabidopsis thaliana OX=3702 GN=PBE1 PE=1 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 7.5e-128
Identity = 222/272 (81.62%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 1   MKLDTSGLQITAPL--FGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSG + + P+  FG   +ML+  S+ PSF++P + +FDGFQK+A  M+K AKGTTT
Sbjct: 1   MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKC 120
           LAFIFK GVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KC
Sbjct: 61  LAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG 180
           R HELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EGGRLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNG 240
            RFSVGSGSPYAYGVLDSGY+YD+SVEEA+ELARR+IYHATFRDGASGGVASVY+VGP G
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPEG 240

Query: 241 WKKLSGDDVGELHYNYYPVTPTTVDQEMAEVT 271
           W KLSGDDVGELHY+YYPV P T +Q M E T
Sbjct: 241 WTKLSGDDVGELHYHYYPVAPATAEQVMEEAT 272

BLAST of Spg033901 vs. ExPASy Swiss-Prot
Match: O55234 (Proteasome subunit beta type-5 OS=Mus musculus OX=10090 GN=Psmb5 PE=1 SV=3)

HSP 1 Score: 291.2 bits (744), Expect = 1.2e-77
Identity = 146/232 (62.93%), Postives = 178/232 (76.72%), Query Frame = 0

Query: 27  AAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSV 86
           AAPS+ +P        ++  I+M+    GTTTLAF F  GV+VAADSRA+ G YI+SQ+V
Sbjct: 42  AAPSWGVP--------EEPRIEML---HGTTTLAFKFLHGVIVAADSRATAGAYIASQTV 101

Query: 87  KKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRRHELANKRRISVAGASKLLANILYSYR 146
           KK+IEINPY+LGTMAGGAADC FW R L  +CR +EL NK RISVA ASKLLAN++Y Y+
Sbjct: 102 KKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYK 161

Query: 147 GMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYRYDLSVEE 206
           GMGLS+GTMI GWD+ GPGLYYVDSEG R+ GT FSVGSGS YAYGV+D GY YDL VEE
Sbjct: 162 GMGLSMGTMICGWDKRGPGLYYVDSEGNRISGTAFSVGSGSVYAYGVMDRGYSYDLKVEE 221

Query: 207 AAELARRAIYHATFRDGASGGVASVYYVGPNGWKKLSGDDVGELHYNYYPVT 259
           A +LARRAIY AT+RD  SGG  ++Y+V  +GW ++S D+V +LH  Y  V+
Sbjct: 222 AYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHDKYSSVS 262

BLAST of Spg033901 vs. ExPASy Swiss-Prot
Match: P28074 (Proteasome subunit beta type-5 OS=Homo sapiens OX=9606 GN=PSMB5 PE=1 SV=3)

HSP 1 Score: 290.4 bits (742), Expect = 2.1e-77
Identity = 144/233 (61.80%), Postives = 177/233 (75.97%), Query Frame = 0

Query: 27  AAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSV 86
           AAP + +P        ++  I+M+    GTTTLAF F+ GV+VAADSRA+ G YI+SQ+V
Sbjct: 42  AAPGWGVP--------EEPGIEML---HGTTTLAFKFRHGVIVAADSRATAGAYIASQTV 101

Query: 87  KKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRRHELANKRRISVAGASKLLANILYSYR 146
           KK+IEINPY+LGTMAGGAADC FW R L  +CR +EL NK RISVA ASKLLAN++Y Y+
Sbjct: 102 KKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYK 161

Query: 147 GMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYRYDLSVEE 206
           GMGLS+GTMI GWD+ GPGLYYVDSEG R+ G  FSVGSGS YAYGV+D GY YDL VE+
Sbjct: 162 GMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQ 221

Query: 207 AAELARRAIYHATFRDGASGGVASVYYVGPNGWKKLSGDDVGELHYNYYPVTP 260
           A +LARRAIY AT+RD  SGG  ++Y+V  +GW ++S D+V +LH  Y   TP
Sbjct: 222 AYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP 263

BLAST of Spg033901 vs. ExPASy TrEMBL
Match: A0A6J1KAW4 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111492198 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 9.5e-150
Identity = 265/272 (97.43%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLQITAPLFGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQ TAPLFGPKME +EGF+AAPSFEIPNSSDFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEFMEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTIA 273
           KLSGDDVGELHYNYYPVTPTTVDQEMAEVT A
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTAA 272

BLAST of Spg033901 vs. ExPASy TrEMBL
Match: A0A6J1HF19 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111462451 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 9.5e-150
Identity = 265/272 (97.43%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLQITAPLFGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQ TAPLFGPKME +EGF+AAPSFEIPNSSDFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEFMEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTIA 273
           KLSGDDVGELHYNYYPVTPTTVDQEMAEVT A
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTAA 272

BLAST of Spg033901 vs. ExPASy TrEMBL
Match: A0A6J1BS30 (Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111004886 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 1.6e-149
Identity = 265/270 (98.15%), Postives = 268/270 (99.26%), Query Frame = 0

Query: 1   MKLDTSGLQITAPLFGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQ TAPLFGPKME+LEGFSAAPSFEIPNS+DFDGFQKEAIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVT 271
           KLSGDDVGELHYNYYPVTP+TVDQEM EVT
Sbjct: 241 KLSGDDVGELHYNYYPVTPSTVDQEMVEVT 270

BLAST of Spg033901 vs. ExPASy TrEMBL
Match: A0A6J1KPP5 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111496110 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 6.1e-149
Identity = 263/268 (98.13%), Postives = 267/268 (99.63%), Query Frame = 0

Query: 1   MKLDTSGLQITAPLFGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQ +APLFGPKME+LEGFSAAPSFE+PNSSDFDGFQKEAIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTSAPLFGPKMEILEGFSAAPSFEVPNSSDFDGFQKEAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAE 269
           KLSGDDVGELHYNYYPVTPTTVDQEMAE
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAE 268

BLAST of Spg033901 vs. ExPASy TrEMBL
Match: A0A5A7VGZ1 (Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00430 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 8.0e-149
Identity = 263/272 (96.69%), Postives = 268/272 (98.53%), Query Frame = 0

Query: 1   MKLDTSGLQITAPLFGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQ TAP+FGP+ME LEGF+AAPSFEIPNSSDFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPMFGPRMEALEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNGWK 240

Query: 241 KLSGDDVGELHYNYYPVTPTTVDQEMAEVTIA 273
           KLSGDDVGELHYNYYPVTPTTVDQEM EVT A
Sbjct: 241 KLSGDDVGELHYNYYPVTPTTVDQEMTEVTAA 272

BLAST of Spg033901 vs. TAIR 10
Match: AT3G26340.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 475.7 bits (1223), Expect = 2.5e-134
Identity = 229/270 (84.81%), Postives = 252/270 (93.33%), Query Frame = 0

Query: 1   MKLDTSGLQITAPL--FGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSGL+ T P+  FG   EML+GFS+APSF++P ++DFDGFQK+A++MVKPAKGTTT
Sbjct: 1   MKLDTSGLETTMPVIGFGSNSEMLDGFSSAPSFDLPRTTDFDGFQKKAVEMVKPAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKC 120
           LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KC
Sbjct: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG 180
           R HELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EGGRLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNG 240
            RFSVGSGSPYAYGVLDSGY++D+SVEEA+ELARR+IYHATFRDGASGGVASVY+VGP G
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPQG 240

Query: 241 WKKLSGDDVGELHYNYYPVTPTTVDQEMAE 269
           W KLSGDDVGELHY+YYPV P T +  M E
Sbjct: 241 WTKLSGDDVGELHYHYYPVAPITAEHVMEE 270

BLAST of Spg033901 vs. TAIR 10
Match: AT1G13060.1 (20S proteasome beta subunit E1 )

HSP 1 Score: 458.0 bits (1177), Expect = 5.3e-129
Identity = 222/272 (81.62%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 1   MKLDTSGLQITAPL--FGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSG + + P+  FG   +ML+  S+ PSF++P + +FDGFQK+A  M+K AKGTTT
Sbjct: 1   MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKC 120
           LAFIFK GVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KC
Sbjct: 61  LAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG 180
           R HELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EGGRLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYYVGPNG 240
            RFSVGSGSPYAYGVLDSGY+YD+SVEEA+ELARR+IYHATFRDGASGGVASVY+VGP G
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPEG 240

Query: 241 WKKLSGDDVGELHYNYYPVTPTTVDQEMAEVT 271
           W KLSGDDVGELHY+YYPV P T +Q M E T
Sbjct: 241 WTKLSGDDVGELHYHYYPVAPATAEQVMEEAT 272

BLAST of Spg033901 vs. TAIR 10
Match: AT1G13060.2 (20S proteasome beta subunit E1 )

HSP 1 Score: 444.5 bits (1142), Expect = 6.1e-125
Identity = 222/296 (75.00%), Postives = 245/296 (82.77%), Query Frame = 0

Query: 1   MKLDTSGLQITAPL--FGPKMEMLEGFSAAPSFEIPNSSDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSG + + P+  FG   +ML+  S+ PSF++P + +FDGFQK+A  M+K AKGTTT
Sbjct: 1   MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYIS------------------------SQSVKKIIEINP 120
           LAFIFK GVMVAADSRASMGGYIS                        SQSVKKIIEINP
Sbjct: 61  LAFIFKGGVMVAADSRASMGGYISVSSLKNSSKNMHPFDIVDFGRNTTSQSVKKIIEINP 120

Query: 121 YMLGTMAGGAADCQFWHRNLGVKCRRHELANKRRISVAGASKLLANILYSYRGMGLSVGT 180
           YMLGTMAGGAADCQFWHRNLG+KCR HELANKRRISV+GASKLLAN+LYSYRGMGLSVGT
Sbjct: 121 YMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGT 180

Query: 181 MIAGWDETGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRA 240
           MIAGWDETGPGLYYVD+EGGRLKG RFSVGSGSPYAYGVLDSGY+YD+SVEEA+ELARR+
Sbjct: 181 MIAGWDETGPGLYYVDNEGGRLKGDRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRS 240

Query: 241 IYHATFRDGASGGVASVYYVGPNGWKKLSGDDVGELHYNYYPVTPTTVDQEMAEVT 271
           IYHATFRDGASGGVASVY+VGP GW KLSGDDVGELHY+YYPV P T +Q M E T
Sbjct: 241 IYHATFRDGASGGVASVYHVGPEGWTKLSGDDVGELHYHYYPVAPATAEQVMEEAT 296

BLAST of Spg033901 vs. TAIR 10
Match: AT4G31300.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 5.3e-20
Identity = 57/185 (30.81%), Postives = 97/185 (52.43%), Query Frame = 0

Query: 55  GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 114
           GTT +   +  GV++ ADSR S G Y+++++  KI ++   +    +G AAD Q     +
Sbjct: 12  GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYV 71

Query: 115 GVKCRRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGG 174
                +H + + +  +V  ++ L+  + Y+ + M L  G ++ GWD+   G  Y    GG
Sbjct: 72  RYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNM-LQTGLIVGGWDKYEGGKIYGIPLGG 131

Query: 175 RLKGTRFSV-GSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYY 234
            +    F++ GSGS Y YG  D  ++ +++ EEA +L  +A+  A  RDGASGGV     
Sbjct: 132 TVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVI 191

Query: 235 VGPNG 239
           +   G
Sbjct: 192 INSEG 195

BLAST of Spg033901 vs. TAIR 10
Match: AT4G31300.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 5.3e-20
Identity = 56/185 (30.27%), Postives = 96/185 (51.89%), Query Frame = 0

Query: 55  GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 114
           GTT +   +  GV++ ADSR S G Y+++++  KI ++   +    +G AAD Q     +
Sbjct: 12  GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYV 71

Query: 115 GVKCRRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGG 174
                +H + + +  +V  ++ L+  + Y+ +   L  G ++ GWD+   G  Y    GG
Sbjct: 72  RYFLHQHTIQHGQPATVKVSANLIRMLAYNNKQNMLQTGLIVGGWDKYEGGKIYGIPLGG 131

Query: 175 RLKGTRFSV-GSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASVYY 234
            +    F++ GSGS Y YG  D  ++ +++ EEA +L  +A+  A  RDGASGGV     
Sbjct: 132 TVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVI 191

Query: 235 VGPNG 239
           +   G
Sbjct: 192 INSEG 196

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038883961.11.5e-14997.79proteasome subunit beta type-5 isoform X1 [Benincasa hispida][more]
XP_022961789.12.0e-14997.43proteasome subunit beta type-5-like [Cucurbita moschata] >XP_022997234.1 proteas... [more]
XP_022131817.13.3e-14998.15proteasome subunit beta type-5 [Momordica charantia] >XP_022131818.1 proteasome ... [more]
XP_023002169.11.3e-14898.13proteasome subunit beta type-5-B isoform X1 [Cucurbita maxima][more]
XP_023538613.11.3e-14898.13proteasome subunit beta type-5-B isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LIP23.5e-13384.81Proteasome subunit beta type-5-B OS=Arabidopsis thaliana OX=3702 GN=PBE2 PE=1 SV... [more]
O243612.9e-13283.64Proteasome subunit beta type-5 OS=Spinacia oleracea OX=3562 PE=2 SV=1[more]
O237177.5e-12881.62Proteasome subunit beta type-5-A OS=Arabidopsis thaliana OX=3702 GN=PBE1 PE=1 SV... [more]
O552341.2e-7762.93Proteasome subunit beta type-5 OS=Mus musculus OX=10090 GN=Psmb5 PE=1 SV=3[more]
P280742.1e-7761.80Proteasome subunit beta type-5 OS=Homo sapiens OX=9606 GN=PSMB5 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1KAW49.5e-15097.43Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111492198 PE=3 SV=1[more]
A0A6J1HF199.5e-15097.43Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111462451 PE=3 SV=1[more]
A0A6J1BS301.6e-14998.15Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111004886 PE=3 SV=1[more]
A0A6J1KPP56.1e-14998.13Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111496110 PE=3 SV=1[more]
A0A5A7VGZ18.0e-14996.69Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
Match NameE-valueIdentityDescription
AT3G26340.12.5e-13484.81N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT1G13060.15.3e-12981.6220S proteasome beta subunit E1 [more]
AT1G13060.26.1e-12575.0020S proteasome beta subunit E1 [more]
AT4G31300.15.3e-2030.81N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G31300.25.3e-2030.27N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000243Peptidase T1A, proteasome beta-subunitPRINTSPR00141PROTEASOMEcoord: 195..206
score: 51.89
coord: 63..78
score: 58.24
coord: 184..195
score: 71.78
coord: 220..231
score: 53.98
IPR001353Proteasome, subunit alpha/betaPFAMPF00227Proteasomecoord: 54..234
e-value: 7.5E-49
score: 165.8
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 56..264
e-value: 1.5E-74
score: 252.5
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 48..254
NoneNo IPR availablePANTHERPTHR11599:SF154PROTEASOME SUBUNIT BETAcoord: 29..269
NoneNo IPR availablePANTHERPTHR11599PROTEASOME SUBUNIT ALPHA/BETAcoord: 29..269
NoneNo IPR availableCDDcd03761proteasome_beta_type_5coord: 56..243
e-value: 7.23566E-132
score: 368.881
IPR016050Proteasome beta-type subunit, conserved sitePROSITEPS00854PROTEASOME_BETA_1coord: 59..106
IPR023333Proteasome B-type subunitPROSITEPS51476PROTEASOME_BETA_2coord: 55..234
score: 55.593914

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg033901.1Spg033901.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010498 proteasomal protein catabolic process
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0019774 proteasome core complex, beta-subunit complex
cellular_component GO:0005839 proteasome core complex
molecular_function GO:0004298 threonine-type endopeptidase activity